Rh3CG295200

Regulator of Vps4 activity in the MVB pathway

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3C
OG0010577
Physical Location & Seq
Reverse (-)
30166564 .. 30167607
1044 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3CG295200.1

Sequence Viewer

Length: 957 bp
ATGTGGGGGTGGGGGTGGGGGTGGGGAAAAGCTTCAAGGTGCAAGAAACTACTCAAACAAGTCCGGTCCCGGCTCGAGTGCTTGAAAGAGGAAAGGTCTACTATTTCTAGAGGGTTAGGGAAAGATGTTGAGGAGCTAGACTACAAGGCACTGAACCTCAGGGTCGAACAGGAACAGCATATCAGAGACGAATCTTCGGTGTACACTTTGCTCGACCATTTCTGCAAGCTCATCCTCATGCACTTCTTTTATATACGAACGCACAGGCAATGTCGTCAAAGAGACCTAGCTGTAGCAGTTTCAAGTCTTGTCTTTGCTTCTTCAAGCTTAGGACGTGGGGTGATTCCTGAGCTTGAGGAGATCAGGGACTTACTCGGAGAGCGTTACGGGGAGAGTTTTGTTGAAACTGCTGTGAAGTTGTTGCCTGGGAATCTTGTAAACCCTAACTTCCAGAAGAATATTCTCGCTCTCTCCCCAGAAACAGTGGTGCCTAATGATATAGTAAAACATGAACTGGTTAAACCCTCTTTTACTACTTCTTCTCCTGATGAGGCACCAGAAAGATCATCAGCAGAGAAAAAGGACGACGAGAATCGGAACAATGAATTACAACTTATCAGCTTACAATCTCCTATGCATGTCCACCCCAAATTGCCTGATTGGGATGATATATTTACTAAGTTCAGTGCTCTCAAGAAGCCTAAGTATTTGCACCCTAAGTTGCCGGAATGTGATGATATTACAGCCAAGTTTAATGCTCTCAAGATCCCCATGAATGAGAACCCTCCTAATAACTACCAGTTGCAATTAGAGTACCATGACGATCATGACAATATTGATGATAGAGCCATATTCGACTATTCCAGGCTCTCGATTGAGAGCAGCTGCAATTTGATAAGCCCGTCCCCAATGTTGTTATTAACGCAATCACCCCATATTGAATACCTAGCTTATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
Ist1

Protein Analysis

318

Amino Acids

36.67

Weight (kDa)

5.91

Isoelectric Point (pI)

61.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ist1 PF03398 15 - 160 2.3e-19 Regulator of Vps4 activity in the MVB pathway
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 487, 553
AccI GTMKAC 1 cut(s) 98
AclWI GGATC 1 cut(s) 760
AfaI GTAC 2 cut(s) 203, 815
AgsI TTSAA 6 cut(s) 36, 85, 303, 324, 404, 941
AjiI CACGTC 1 cut(s) 335
AjnI CCWGG 2 cut(s) 424, 863
AluBI AGCT 9 cut(s) 32, 136, 229, 290, 327, 352, 621, 885, 950
AluI AGCT 9 cut(s) 32, 136, 229, 290, 327, 352, 621, 885, 950
Alw21I GWGCWC 1 cut(s) 691
Alw26I GTCTC 2 cut(s) 180, 276
AlwI GGATC 1 cut(s) 760
Ama87I CYCGRG 1 cut(s) 74
ApeKI GCWGC 2 cut(s) 882, 885
Asp700I GAANNNNTTC 1 cut(s) 31
AspS9I GGNCC 1 cut(s) 66
AsuC2I CCSGG 1 cut(s) 70
AsuHPI GGTGA 2 cut(s) 352, 921
AvaI CYCGRG 1 cut(s) 74
AvaII GGWCC 1 cut(s) 66
AxyI CCTNAGG 1 cut(s) 158
BanI GGYRCC 2 cut(s) 487, 553
Bbv12I GWGCWC 1 cut(s) 691
BbvI GCAGC 2 cut(s) 872, 894
BciT130I CCWGG 2 cut(s) 426, 865
BcnI CCSGG 1 cut(s) 70
BcoDI GTCTC 2 cut(s) 180, 276
BfaI CTAG 4 cut(s) 108, 137, 287, 947
BfmI CTRYAG 1 cut(s) 291
BisI GCNGC 2 cut(s) 883, 886
BlsI GCNGC 2 cut(s) 884, 887
Bme1390I CCNGG 3 cut(s) 70, 426, 865
Bme18I GGWCC 1 cut(s) 66
BmeT110I CYCGRG 1 cut(s) 74
BmgBI CACGTC 1 cut(s) 335
BmgT120I GGNCC 1 cut(s) 66
BmiI GGNNCC 3 cut(s) 68, 489, 555
BmrFI CCNGG 3 cut(s) 70, 426, 865
Bpu10I CCTNAGC 2 cut(s) 328, 348
BpuEI CTTGAG 3 cut(s) 374, 677, 746
BpuMI CCSGG 1 cut(s) 70
BsaI GGTCTC 1 cut(s) 276
BsaJI CCNNGG 1 cut(s) 425
BsaWI WCCGGW 1 cut(s) 63
BsaXI ACNNNNNCTCC 2 cut(s) 526, 556
Bse1I ACTGG 2 cut(s) 519, 799
Bse21I CCTNAGG 1 cut(s) 158
Bse3DI GCAATG 1 cut(s) 275
BseBI CCWGG 2 cut(s) 426, 865
BseDI CCNNGG 1 cut(s) 425
BseGI GGATG 2 cut(s) 231, 670
BseMI GCAATG 1 cut(s) 275
BseMII CTCAG 2 cut(s) 172, 339
BseNI ACTGG 2 cut(s) 519, 799
BseRI GAGGAG 2 cut(s) 146, 371
BseXI GCAGC 2 cut(s) 872, 894
BshNI GGYRCC 2 cut(s) 487, 553
BsiHKAI GWGCWC 1 cut(s) 691
BsiHKCI CYCGRG 1 cut(s) 74
BsiSI CCGG 3 cut(s) 64, 70, 725
BslFI GGGAC 3 cut(s) 52, 380, 889
BsmAI GTCTC 2 cut(s) 180, 276
BsmBI CGTCTC 1 cut(s) 180
BsmFI GGGAC 3 cut(s) 52, 380, 889
Bso31I GGTCTC 1 cut(s) 276
BsoBI CYCGRG 1 cut(s) 74
Bsp1286I GDGCHC 1 cut(s) 691
Bsp1407I TGTACA 1 cut(s) 201
Bsp143I GATC 4 cut(s) 360, 563, 765, 823
BspCNI CTCAG 2 cut(s) 171, 340
BspHI TCATGA 1 cut(s) 826
BspLI GGNNCC 3 cut(s) 68, 489, 555
BspPI GGATC 1 cut(s) 760
BspT107I GGYRCC 2 cut(s) 487, 553
BspTNI GGTCTC 1 cut(s) 276
BsrDI GCAATG 1 cut(s) 275
BsrGI TGTACA 1 cut(s) 201
BsrI ACTGG 2 cut(s) 519, 799
BssECI CCNNGG 1 cut(s) 425
BssMI GATC 4 cut(s) 360, 563, 765, 823
Bst2UI CCWGG 2 cut(s) 426, 865
Bst4CI ACNGT 1 cut(s) 484
BstAUI TGTACA 1 cut(s) 201
BstC8I GCNNGC 1 cut(s) 227
BstDEI CTNAG 6 cut(s) 158, 328, 348, 678, 702, 717
BstF5I GGATG 2 cut(s) 231, 670
BstKTI GATC 4 cut(s) 363, 566, 768, 826
BstMAI GTCTC 2 cut(s) 180, 276
BstMBI GATC 4 cut(s) 360, 563, 765, 823
BstNI CCWGG 2 cut(s) 426, 865
BstNSI RCATGY 1 cut(s) 641
BstSCI CCNGG 3 cut(s) 68, 424, 863
BstSFI CTRYAG 1 cut(s) 291
BstV1I GCAGC 2 cut(s) 872, 894
BstX2I RGATCY 1 cut(s) 765
BstYI RGATCY 1 cut(s) 765
Bsu36I CCTNAGG 1 cut(s) 158
BtrI CACGTC 1 cut(s) 335
BtsCI GGATG 2 cut(s) 231, 670
BtsIMutI CAGTG 3 cut(s) 149, 489, 691
Cac8I GCNNGC 1 cut(s) 227
CciI TCATGA 1 cut(s) 826
Cfr13I GGNCC 1 cut(s) 66
Csp6I GTAC 2 cut(s) 202, 814
CviAII CATG 6 cut(s) 238, 509, 638, 772, 818, 827
CviQI GTAC 2 cut(s) 202, 814
DdeI CTNAG 6 cut(s) 158, 328, 348, 678, 702, 717
DpnI GATC 4 cut(s) 362, 565, 767, 825
DpnII GATC 4 cut(s) 360, 563, 765, 823
Eco31I GGTCTC 1 cut(s) 276
Eco47I GGWCC 1 cut(s) 66
Eco81I CCTNAGG 1 cut(s) 158
Eco88I CYCGRG 1 cut(s) 74
EcoRII CCWGG 2 cut(s) 424, 863
EcoT22I ATGCAT 1 cut(s) 639
Esp3I CGTCTC 1 cut(s) 180
FaeI CATG 6 cut(s) 241, 512, 641, 775, 821, 830
FaqI GGGAC 3 cut(s) 52, 380, 889
FatI CATG 6 cut(s) 237, 508, 637, 771, 817, 826
FblI GTMKAC 1 cut(s) 98
Fnu4HI GCNGC 2 cut(s) 883, 886
FokI GGATG 2 cut(s) 218, 677
Fsp4HI GCNGC 2 cut(s) 883, 886
FspBI CTAG 4 cut(s) 108, 137, 287, 947
GluI GCNGC 2 cut(s) 883, 886
HapII CCGG 3 cut(s) 64, 70, 725
Hin1II CATG 6 cut(s) 241, 512, 641, 775, 821, 830
HindIII AAGCTT 2 cut(s) 30, 325
HinfI GANTC 4 cut(s) 191, 343, 430, 592
HpaII CCGG 3 cut(s) 64, 70, 725
HphI GGTGA 2 cut(s) 352, 921
Hpy166II GTNNAC 5 cut(s) 99, 202, 204, 439, 643
Hpy188I TCNGA 3 cut(s) 185, 377, 597
Hpy188III TCNNGA 8 cut(s) 108, 347, 451, 545, 694, 763, 827, 871
Hpy8I GTNNAC 5 cut(s) 99, 202, 204, 439, 643
Hpy99I CGWCG 1 cut(s) 590
HpyCH4III ACNGT 1 cut(s) 484
HpyCH4IV ACGT 1 cut(s) 334
HpyCH4V TGCA 7 cut(s) 42, 225, 241, 637, 712, 805, 888
HpyF3I CTNAG 6 cut(s) 158, 328, 348, 678, 702, 717
HpySE526I ACGT 1 cut(s) 334
Hsp92II CATG 6 cut(s) 241, 512, 641, 775, 821, 830
Kzo9I GATC 4 cut(s) 360, 563, 765, 823
LmnI GCTCC 1 cut(s) 133
Lsp1109I GCAGC 2 cut(s) 872, 894
MaeI CTAG 4 cut(s) 108, 137, 287, 947
MaeII ACGT 1 cut(s) 334
MaeIII GTNAC 1 cut(s) 383
MalI GATC 4 cut(s) 362, 565, 767, 825
MboI GATC 4 cut(s) 360, 563, 765, 823
MboII GAAGA 4 cut(s) 186, 312, 466, 531
MflI RGATCY 1 cut(s) 765
MhlI GDGCHC 1 cut(s) 691
MluCI AATT 4 cut(s) 605, 650, 806, 889
MnlI CCTC 9 cut(s) 82, 104, 124, 167, 245, 349, 535, 544, 795
Mph1103I ATGCAT 1 cut(s) 639
MroXI GAANNNNTTC 1 cut(s) 31
MseI TTAA 3 cut(s) 519, 753, 920
MslI CAYNNNNRTG 1 cut(s) 236
MspA1I CMGCKG 1 cut(s) 885
MspI CCGG 3 cut(s) 64, 70, 725
MspR9I CCNGG 3 cut(s) 70, 426, 865
MvaI CCWGG 2 cut(s) 426, 865
NciI CCSGG 1 cut(s) 70
NdeII GATC 4 cut(s) 360, 563, 765, 823
NlaIII CATG 6 cut(s) 241, 512, 641, 775, 821, 830
NlaIV GGNNCC 3 cut(s) 68, 489, 555
NsiI ATGCAT 1 cut(s) 639
NspI RCATGY 1 cut(s) 641
PaeR7I CTCGAG 1 cut(s) 74
PagI TCATGA 1 cut(s) 826
PdmI GAANNNNTTC 1 cut(s) 31
PfeI GAWTC 4 cut(s) 191, 343, 430, 592
PkrI GCNGC 2 cut(s) 884, 887
Psp6I CCWGG 2 cut(s) 424, 863
PspGI CCWGG 2 cut(s) 424, 863
PspN4I GGNNCC 3 cut(s) 68, 489, 555
PspPI GGNCC 1 cut(s) 66
PspXI VCTCGAGB 1 cut(s) 74
PsuI RGATCY 1 cut(s) 765
PvuII CAGCTG 1 cut(s) 885
RsaI GTAC 2 cut(s) 203, 815
RsaNI GTAC 2 cut(s) 202, 814
RseI CAYNNNNRTG 1 cut(s) 236
SaqAI TTAA 3 cut(s) 519, 753, 920
SatI GCNGC 2 cut(s) 883, 886
Sau3AI GATC 4 cut(s) 360, 563, 765, 823
Sau96I GGNCC 1 cut(s) 66
ScrFI CCNGG 3 cut(s) 70, 426, 865
SduI GDGCHC 1 cut(s) 691
SfcI CTRYAG 1 cut(s) 291
Sfr274I CTCGAG 1 cut(s) 74
SinI GGWCC 1 cut(s) 66
SlaI CTCGAG 1 cut(s) 74
SmiMI CAYNNNNRTG 1 cut(s) 236
SmlI CTYRAG 4 cut(s) 74, 353, 692, 761
SmoI CTYRAG 4 cut(s) 74, 353, 692, 761
Sse9I AATT 4 cut(s) 605, 650, 806, 889
SspI AATATT 2 cut(s) 460, 835
SspMI CTAG 4 cut(s) 108, 137, 287, 947
StyD4I CCNGG 3 cut(s) 68, 424, 863
TaaI ACNGT 1 cut(s) 484
TaiI ACGT 1 cut(s) 337
TaqI TCGA 5 cut(s) 75, 165, 213, 855, 872
TasI AATT 4 cut(s) 605, 650, 806, 889
TatI WGTACW 1 cut(s) 201
TfiI GAWTC 4 cut(s) 191, 343, 430, 592
Tru1I TTAA 3 cut(s) 519, 753, 920
Tru9I TTAA 3 cut(s) 519, 753, 920
TscAI CASTG 3 cut(s) 156, 489, 691
TseI GCWGC 2 cut(s) 882, 885
TspDTI ATGAA 3 cut(s) 525, 618, 788
TspRI CASTG 3 cut(s) 156, 489, 691
VpaK11BI GGWCC 1 cut(s) 66
XbaI TCTAGA 1 cut(s) 107
XceI RCATGY 1 cut(s) 641
XhoI CTCGAG 1 cut(s) 74
XmiI GTMKAC 1 cut(s) 98
XmnI GAANNNNTTC 1 cut(s) 31
XspI CTAG 4 cut(s) 108, 137, 287, 947
Zsp2I ATGCAT 1 cut(s) 639
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.