Rroxscaffold_6G00397600

Regulator of Vps4 activity in the MVB pathway

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0010577
Physical Location & Seq
Forward (+)
19299305 .. 19300626
1322 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00397600.1

Sequence Viewer

Length: 750 bp
ATGTGGGGATGGGGGTGGGGGTGGGGAAAAGCTTCAAGGTGCAAGAAACTACTCAAACAAGTCCGGTCCCGGCTCGAGTGCTTGAAAGAGGAAAGGTCTACTATTTCTACAGAGTTAGGGAAAGATGTTGAGGAGCTAGACTACAAGGCACTGAACCTCAGGGTCGAACAAGAACAGCACATCAGAGACGAATCTTCGGTGTACACTTTGCTCGACCATTTCTGCAAGCTCATCCTCATGCACTTCTTTTATATCCGAACGCACAAGCAATGTCGTCCAAGAGACCTAGCTGTAGCAGTTTCAAGTCTTGTCTTTGCTTCTTCAAGATTAAGACGCGGGGTGATTCCTGAGCTTGGGGAGATCAGGGACTTGCTCGGAGAGCGTTACGGGGAGAGTTTTGTTGAAACTGCTGTGAAGTTGTTGCCTGGGAATCTTGTAAACCCTAACTTCCAGAAGAATATTCTCGCTCTCTCCCCAGAAACAGTGGTGCCTAATGATATAGTAAAACATGAACTGGTTAAATCCTCTTTTACTACTTCTCCTGATGAGGCACCAGAAAGATCATCAGCAGAGAAAAAGGACGACGAGAATCGGAACAATGAATTACAACTTATCAGCTTACAATCTCCTATGCATGTCCACCCCAAATTGCCTGATTGGGATGATATATTTACTAAGTTCAGTGCACTCAAGAAGCCTAGGTCAGATGTTTTGGCTCACAAGACGACTTTAAGCCATCACATATGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
Ist1

Protein Analysis

249

Amino Acids

28.74

Weight (kDa)

8.28

Isoelectric Point (pI)

58.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ist1 PF03398 15 - 161 2.4e-20 Regulator of Vps4 activity in the MVB pathway
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 487, 550
AccI GTMKAC 1 cut(s) 98
AccII CGCG 1 cut(s) 336
AciI CCGC 1 cut(s) 336
AfaI GTAC 1 cut(s) 203
AfiI CCNNNNNNNGG 1 cut(s) 353
AgsI TTSAA 5 cut(s) 36, 85, 303, 324, 404
AjnI CCWGG 1 cut(s) 424
AluBI AGCT 6 cut(s) 32, 136, 229, 290, 352, 618
AluI AGCT 6 cut(s) 32, 136, 229, 290, 352, 618
Alw21I GWGCWC 1 cut(s) 688
Alw26I GTCTC 2 cut(s) 180, 276
Alw44I GTGCAC 1 cut(s) 684
Ama87I CYCGRG 1 cut(s) 74
ApaLI GTGCAC 1 cut(s) 684
Asp700I GAANNNNTTC 1 cut(s) 31
AspA2I CCTAGG 1 cut(s) 698
AspS9I GGNCC 1 cut(s) 66
AsuC2I CCSGG 1 cut(s) 70
AsuHPI GGTGA 1 cut(s) 352
AvaI CYCGRG 1 cut(s) 74
AvaII GGWCC 1 cut(s) 66
AvrII CCTAGG 1 cut(s) 698
AxyI CCTNAGG 1 cut(s) 158
BaeGI GKGCMC 1 cut(s) 688
BanI GGYRCC 2 cut(s) 487, 550
Bbv12I GWGCWC 1 cut(s) 688
BccI CCATC 2 cut(s) 3, 744
BciT130I CCWGG 1 cut(s) 426
BcnI CCSGG 1 cut(s) 70
BcoDI GTCTC 2 cut(s) 180, 276
BfaI CTAG 3 cut(s) 137, 287, 699
BfmI CTRYAG 2 cut(s) 108, 291
BlnI CCTAGG 1 cut(s) 698
Bme1390I CCNGG 2 cut(s) 70, 426
Bme18I GGWCC 1 cut(s) 66
BmeT110I CYCGRG 1 cut(s) 74
BmgT120I GGNCC 1 cut(s) 66
BmiI GGNNCC 3 cut(s) 68, 489, 552
BmrFI CCNGG 2 cut(s) 70, 426
Bpu10I CCTNAGC 1 cut(s) 348
BpuEI CTTGAG 1 cut(s) 674
BpuMI CCSGG 1 cut(s) 70
BsaI GGTCTC 1 cut(s) 276
BsaJI CCNNGG 2 cut(s) 425, 698
BsaWI WCCGGW 1 cut(s) 63
BsaXI ACNNNNNCTCC 2 cut(s) 523, 553
Bsc4I CCNNNNNNNGG 1 cut(s) 353
Bse1I ACTGG 1 cut(s) 519
Bse21I CCTNAGG 1 cut(s) 158
Bse3DI GCAATG 1 cut(s) 275
BseBI CCWGG 1 cut(s) 426
BseDI CCNNGG 2 cut(s) 425, 698
BseGI GGATG 3 cut(s) 14, 231, 667
BseLI CCNNNNNNNGG 1 cut(s) 353
BseMI GCAATG 1 cut(s) 275
BseMII CTCAG 2 cut(s) 172, 339
BseNI ACTGG 1 cut(s) 519
BseRI GAGGAG 1 cut(s) 146
BseSI GKGCMC 1 cut(s) 688
Bsh1236I CGCG 1 cut(s) 336
BshNI GGYRCC 2 cut(s) 487, 550
BsiHKAI GWGCWC 1 cut(s) 688
BsiHKCI CYCGRG 1 cut(s) 74
BsiSI CCGG 2 cut(s) 64, 70
BslFI GGGAC 2 cut(s) 52, 380
BslI CCNNNNNNNGG 1 cut(s) 353
BsmAI GTCTC 2 cut(s) 180, 276
BsmBI CGTCTC 1 cut(s) 180
BsmFI GGGAC 2 cut(s) 52, 380
Bso31I GGTCTC 1 cut(s) 276
BsoBI CYCGRG 1 cut(s) 74
Bsp1286I GDGCHC 1 cut(s) 688
Bsp1407I TGTACA 1 cut(s) 201
Bsp143I GATC 2 cut(s) 360, 560
BspACI CCGC 1 cut(s) 336
BspCNI CTCAG 2 cut(s) 171, 340
BspFNI CGCG 1 cut(s) 336
BspLI GGNNCC 3 cut(s) 68, 489, 552
BspT107I GGYRCC 2 cut(s) 487, 550
BspTNI GGTCTC 1 cut(s) 276
BsrDI GCAATG 1 cut(s) 275
BsrGI TGTACA 1 cut(s) 201
BsrI ACTGG 1 cut(s) 519
BssECI CCNNGG 2 cut(s) 425, 698
BssMI GATC 2 cut(s) 360, 560
BssT1I CCWWGG 1 cut(s) 698
Bst2UI CCWGG 1 cut(s) 426
Bst4CI ACNGT 1 cut(s) 484
BstAUI TGTACA 1 cut(s) 201
BstC8I GCNNGC 1 cut(s) 227
BstDEI CTNAG 3 cut(s) 158, 348, 675
BstF5I GGATG 3 cut(s) 14, 231, 667
BstFNI CGCG 1 cut(s) 336
BstKTI GATC 2 cut(s) 363, 563
BstMAI GTCTC 2 cut(s) 180, 276
BstMBI GATC 2 cut(s) 360, 560
BstMWI GCNNNNNNNGC 1 cut(s) 379
BstNI CCWGG 1 cut(s) 426
BstNSI RCATGY 1 cut(s) 638
BstSCI CCNGG 2 cut(s) 68, 424
BstSFI CTRYAG 2 cut(s) 108, 291
BstSLI GKGCMC 1 cut(s) 688
BstUI CGCG 1 cut(s) 336
Bsu36I CCTNAGG 1 cut(s) 158
BtsCI GGATG 3 cut(s) 14, 231, 667
BtsIMutI CAGTG 3 cut(s) 149, 489, 688
Cac8I GCNNGC 1 cut(s) 227
Cfr13I GGNCC 1 cut(s) 66
CseI GACGC 1 cut(s) 342
Csp6I GTAC 1 cut(s) 202
CviAII CATG 3 cut(s) 238, 509, 635
CviQI GTAC 1 cut(s) 202
DdeI CTNAG 3 cut(s) 158, 348, 675
DpnI GATC 2 cut(s) 362, 562
DpnII GATC 2 cut(s) 360, 560
Eco130I CCWWGG 1 cut(s) 698
Eco31I GGTCTC 1 cut(s) 276
Eco47I GGWCC 1 cut(s) 66
Eco81I CCTNAGG 1 cut(s) 158
Eco88I CYCGRG 1 cut(s) 74
EcoRII CCWGG 1 cut(s) 424
EcoT14I CCWWGG 1 cut(s) 698
EcoT22I ATGCAT 1 cut(s) 636
ErhI CCWWGG 1 cut(s) 698
Esp3I CGTCTC 1 cut(s) 180
FaeI CATG 3 cut(s) 241, 512, 638
FaiI YATR 9 cut(s) 239, 252, 500, 510, 632, 636, 668, 743, 745
FaqI GGGAC 2 cut(s) 52, 380
FatI CATG 3 cut(s) 237, 508, 634
FauI CCCGC 1 cut(s) 329
FauNDI CATATG 1 cut(s) 743
FblI GTMKAC 1 cut(s) 98
FokI GGATG 3 cut(s) 21, 218, 674
FspBI CTAG 3 cut(s) 137, 287, 699
HapII CCGG 2 cut(s) 64, 70
HgaI GACGC 1 cut(s) 342
Hin1II CATG 3 cut(s) 241, 512, 638
HindIII AAGCTT 1 cut(s) 30
HinfI GANTC 4 cut(s) 191, 343, 430, 589
HpaII CCGG 2 cut(s) 64, 70
HphI GGTGA 1 cut(s) 352
Hpy166II GTNNAC 6 cut(s) 99, 202, 204, 439, 640, 686
Hpy188I TCNGA 5 cut(s) 185, 257, 377, 594, 706
Hpy188III TCNNGA 5 cut(s) 324, 347, 451, 542, 691
Hpy8I GTNNAC 6 cut(s) 99, 202, 204, 439, 640, 686
Hpy99I CGWCG 1 cut(s) 587
HpyCH4III ACNGT 1 cut(s) 484
HpyCH4V TGCA 5 cut(s) 42, 225, 241, 634, 686
HpyF10VI GCNNNNNNNGC 1 cut(s) 379
HpyF3I CTNAG 3 cut(s) 158, 348, 675
Hsp92II CATG 3 cut(s) 241, 512, 638
Kzo9I GATC 2 cut(s) 360, 560
LmnI GCTCC 1 cut(s) 133
MaeI CTAG 3 cut(s) 137, 287, 699
MaeIII GTNAC 1 cut(s) 383
MalI GATC 2 cut(s) 362, 562
MboI GATC 2 cut(s) 360, 560
MboII GAAGA 3 cut(s) 186, 312, 466
MhlI GDGCHC 1 cut(s) 688
MluCI AATT 2 cut(s) 602, 647
MnlI CCTC 6 cut(s) 82, 124, 167, 245, 535, 541
Mph1103I ATGCAT 1 cut(s) 636
MroXI GAANNNNTTC 1 cut(s) 31
MseI TTAA 3 cut(s) 329, 519, 731
MslI CAYNNNNRTG 1 cut(s) 236
MspI CCGG 2 cut(s) 64, 70
MspR9I CCNGG 2 cut(s) 70, 426
MvaI CCWGG 1 cut(s) 426
MvnI CGCG 1 cut(s) 336
MwoI GCNNNNNNNGC 1 cut(s) 379
NciI CCSGG 1 cut(s) 70
NdeI CATATG 1 cut(s) 743
NdeII GATC 2 cut(s) 360, 560
NlaIII CATG 3 cut(s) 241, 512, 638
NlaIV GGNNCC 3 cut(s) 68, 489, 552
NsiI ATGCAT 1 cut(s) 636
NspI RCATGY 1 cut(s) 638
PaeR7I CTCGAG 1 cut(s) 74
PdmI GAANNNNTTC 1 cut(s) 31
PfeI GAWTC 4 cut(s) 191, 343, 430, 589
Psp6I CCWGG 1 cut(s) 424
PspGI CCWGG 1 cut(s) 424
PspN4I GGNNCC 3 cut(s) 68, 489, 552
PspPI GGNCC 1 cut(s) 66
PspXI VCTCGAGB 1 cut(s) 74
RsaI GTAC 1 cut(s) 203
RsaNI GTAC 1 cut(s) 202
RseI CAYNNNNRTG 1 cut(s) 236
SaqAI TTAA 3 cut(s) 329, 519, 731
Sau3AI GATC 2 cut(s) 360, 560
Sau96I GGNCC 1 cut(s) 66
ScrFI CCNGG 2 cut(s) 70, 426
SduI GDGCHC 1 cut(s) 688
SfcI CTRYAG 2 cut(s) 108, 291
Sfr274I CTCGAG 1 cut(s) 74
SinI GGWCC 1 cut(s) 66
SlaI CTCGAG 1 cut(s) 74
SmiMI CAYNNNNRTG 1 cut(s) 236
SmlI CTYRAG 2 cut(s) 74, 689
SmoI CTYRAG 2 cut(s) 74, 689
Sse9I AATT 2 cut(s) 602, 647
SsiI CCGC 1 cut(s) 336
SspI AATATT 1 cut(s) 460
SspMI CTAG 3 cut(s) 137, 287, 699
StyD4I CCNGG 2 cut(s) 68, 424
StyI CCWWGG 1 cut(s) 698
TaaI ACNGT 1 cut(s) 484
TaqI TCGA 3 cut(s) 75, 165, 213
TasI AATT 2 cut(s) 602, 647
TatI WGTACW 1 cut(s) 201
TfiI GAWTC 4 cut(s) 191, 343, 430, 589
Tru1I TTAA 3 cut(s) 329, 519, 731
Tru9I TTAA 3 cut(s) 329, 519, 731
TscAI CASTG 3 cut(s) 156, 489, 688
TspDTI ATGAA 2 cut(s) 525, 615
TspRI CASTG 3 cut(s) 156, 489, 688
VneI GTGCAC 1 cut(s) 684
VpaK11BI GGWCC 1 cut(s) 66
XceI RCATGY 1 cut(s) 638
XhoI CTCGAG 1 cut(s) 74
XmaJI CCTAGG 1 cut(s) 698
XmiI GTMKAC 1 cut(s) 98
XmnI GAANNNNTTC 1 cut(s) 31
XspI CTAG 3 cut(s) 137, 287, 699
Zsp2I ATGCAT 1 cut(s) 636
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.