RLG00000023216

Regulator of Vps4 activity in the MVB pathway

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0010577
Physical Location & Seq
Forward (+)
20077051 .. 20078004
954 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000023216

Sequence Viewer

Length: 954 bp
ATGTGGGGGTGGGGGTGGGGGTGGGGAAAAGCTTCAAGGTGCAAGAAACTACTCAAACAAGTCCGGTCCCGGCTCGAGTGCTTGAAAGAGGAAAGGTCTACTATTTCTACAGAGTTAGGGAAAGATGTTGAGGAGCTAGACTACAAGGCACTGAACCTCAGGGTCGAACAAGAACAGCACATCAGAGACGAATCTTCGGTGTACACTTTGCTCGACCATTTCTGCAAGCTCATCCTCATGCACTTCTTTTATATCCGAACGCACAAGCAATGTCGTCCAAGAGACCTAGCTGTAGCAGTTTCAAGTCTTGTCTTTGCTTCTTCAAGATTAAGAGGCGGGGTGATTCCTGAGCTTGAGGAGATCAGGGACTTACTCGGAGAGCGTTACGGGGAGAGTTTTGTTGAAACTGCTGTGAAGTTGTTGCCTGGGAATCTTGTAAACCCTAACTTCCAGAAGAATATTCTCGCTCTCTCCCCAGAAACAGTGGTGCCTAATGATATAGTAAATCATGAACTGGTTAAACCCTCTTTTACTACTTCTTCTCCTGATGAGGCACCAGAAAGATCATCAGAAGAGAAAAAGGACGACGAGAATCGGAACAATGAATTACAACTTATCAGCTTACAATCTCCTATGCATGTCCACCCCAAATTGCCTGATTGGGATGATATATTTACTAAGTTCAGTGCTCTCAAGAAGCCTAAGTATTTGCACCCTAAGTTGCCAAAATATGATGATATTACAGCCAAGTTTAATGCTCTCAAGATCCCCATGAATGAGAACCCTCCTAATAACTACCAGTTGCAATTAGAGTACCATGATGATCATGACAATATTGATGATAGAGCCATATTCGACTATTCACGGCTCTCGATTGAGAGCAGCTGCAATTTGATAAGCCCGTCCCCATTGTTATTAACGCAATCACCCCATATTGAATACCTAGCTTATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
Ist1

Protein Analysis

318

Amino Acids

36.67

Weight (kDa)

5.81

Isoelectric Point (pI)

63.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ist1 PF03398 15 - 160 1.7e-20 Regulator of Vps4 activity in the MVB pathway
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 487, 553
AccI GTMKAC 1 cut(s) 98
AciI CCGC 1 cut(s) 336
AclWI GGATC 1 cut(s) 760
AfaI GTAC 2 cut(s) 203, 815
AgsI TTSAA 6 cut(s) 36, 85, 303, 324, 404, 938
AjnI CCWGG 1 cut(s) 424
AluBI AGCT 8 cut(s) 32, 136, 229, 290, 352, 621, 885, 947
AluI AGCT 8 cut(s) 32, 136, 229, 290, 352, 621, 885, 947
Alw21I GWGCWC 1 cut(s) 691
Alw26I GTCTC 2 cut(s) 180, 276
AlwI GGATC 1 cut(s) 760
Ama87I CYCGRG 1 cut(s) 74
ApeKI GCWGC 2 cut(s) 882, 885
Asp700I GAANNNNTTC 1 cut(s) 31
AspS9I GGNCC 1 cut(s) 66
AsuC2I CCSGG 1 cut(s) 70
AsuHPI GGTGA 2 cut(s) 352, 918
AvaI CYCGRG 1 cut(s) 74
AvaII GGWCC 1 cut(s) 66
AxyI CCTNAGG 1 cut(s) 158
BanI GGYRCC 2 cut(s) 487, 553
Bbv12I GWGCWC 1 cut(s) 691
BbvI GCAGC 2 cut(s) 872, 894
BceAI ACGGC 1 cut(s) 881
BciT130I CCWGG 1 cut(s) 426
BclI TGATCA 1 cut(s) 823
BcnI CCSGG 1 cut(s) 70
BcoDI GTCTC 2 cut(s) 180, 276
BfaI CTAG 3 cut(s) 137, 287, 944
BfmI CTRYAG 2 cut(s) 108, 291
BisI GCNGC 2 cut(s) 883, 886
BlsI GCNGC 2 cut(s) 884, 887
Bme1390I CCNGG 2 cut(s) 70, 426
Bme18I GGWCC 1 cut(s) 66
BmeT110I CYCGRG 1 cut(s) 74
BmgT120I GGNCC 1 cut(s) 66
BmiI GGNNCC 3 cut(s) 68, 489, 555
BmrFI CCNGG 2 cut(s) 70, 426
Bpu10I CCTNAGC 1 cut(s) 348
BpuEI CTTGAG 3 cut(s) 374, 677, 746
BpuMI CCSGG 1 cut(s) 70
BsaI GGTCTC 1 cut(s) 276
BsaJI CCNNGG 1 cut(s) 425
BsaWI WCCGGW 1 cut(s) 63
BsaXI ACNNNNNCTCC 2 cut(s) 526, 556
Bse1I ACTGG 2 cut(s) 519, 799
Bse21I CCTNAGG 1 cut(s) 158
Bse3DI GCAATG 1 cut(s) 275
BseBI CCWGG 1 cut(s) 426
BseDI CCNNGG 1 cut(s) 425
BseGI GGATG 2 cut(s) 231, 670
BseMI GCAATG 1 cut(s) 275
BseMII CTCAG 2 cut(s) 172, 339
BseNI ACTGG 2 cut(s) 519, 799
BseRI GAGGAG 2 cut(s) 146, 371
BseXI GCAGC 2 cut(s) 872, 894
BshNI GGYRCC 2 cut(s) 487, 553
BsiHKAI GWGCWC 1 cut(s) 691
BsiHKCI CYCGRG 1 cut(s) 74
BsiSI CCGG 2 cut(s) 64, 70
BslFI GGGAC 3 cut(s) 52, 380, 889
BsmAI GTCTC 2 cut(s) 180, 276
BsmBI CGTCTC 1 cut(s) 180
BsmFI GGGAC 3 cut(s) 52, 380, 889
Bso31I GGTCTC 1 cut(s) 276
BsoBI CYCGRG 1 cut(s) 74
Bsp1286I GDGCHC 1 cut(s) 691
Bsp1407I TGTACA 1 cut(s) 201
Bsp143I GATC 4 cut(s) 360, 563, 765, 823
BspACI CCGC 1 cut(s) 336
BspCNI CTCAG 2 cut(s) 171, 340
BspHI TCATGA 2 cut(s) 508, 826
BspLI GGNNCC 3 cut(s) 68, 489, 555
BspPI GGATC 1 cut(s) 760
BspT107I GGYRCC 2 cut(s) 487, 553
BspTNI GGTCTC 1 cut(s) 276
BsrDI GCAATG 1 cut(s) 275
BsrGI TGTACA 1 cut(s) 201
BsrI ACTGG 2 cut(s) 519, 799
BssECI CCNNGG 1 cut(s) 425
BssMI GATC 4 cut(s) 360, 563, 765, 823
Bst2UI CCWGG 1 cut(s) 426
Bst4CI ACNGT 1 cut(s) 484
Bst6I CTCTTC 1 cut(s) 567
BstAUI TGTACA 1 cut(s) 201
BstC8I GCNNGC 1 cut(s) 227
BstDEI CTNAG 5 cut(s) 158, 348, 678, 702, 717
BstF5I GGATG 2 cut(s) 231, 670
BstKTI GATC 4 cut(s) 363, 566, 768, 826
BstMAI GTCTC 2 cut(s) 180, 276
BstMBI GATC 4 cut(s) 360, 563, 765, 823
BstNI CCWGG 1 cut(s) 426
BstNSI RCATGY 1 cut(s) 641
BstSCI CCNGG 2 cut(s) 68, 424
BstSFI CTRYAG 2 cut(s) 108, 291
BstV1I GCAGC 2 cut(s) 872, 894
BstX2I RGATCY 1 cut(s) 765
BstYI RGATCY 1 cut(s) 765
Bsu36I CCTNAGG 1 cut(s) 158
BtsCI GGATG 2 cut(s) 231, 670
BtsIMutI CAGTG 3 cut(s) 149, 489, 691
Cac8I GCNNGC 1 cut(s) 227
CciI TCATGA 2 cut(s) 508, 826
Cfr13I GGNCC 1 cut(s) 66
Csp6I GTAC 2 cut(s) 202, 814
CviAII CATG 6 cut(s) 238, 509, 638, 772, 818, 827
CviQI GTAC 2 cut(s) 202, 814
DdeI CTNAG 5 cut(s) 158, 348, 678, 702, 717
DpnI GATC 4 cut(s) 362, 565, 767, 825
DpnII GATC 4 cut(s) 360, 563, 765, 823
Eam1104I CTCTTC 1 cut(s) 567
EarI CTCTTC 1 cut(s) 567
Eco31I GGTCTC 1 cut(s) 276
Eco47I GGWCC 1 cut(s) 66
Eco81I CCTNAGG 1 cut(s) 158
Eco88I CYCGRG 1 cut(s) 74
EcoRII CCWGG 1 cut(s) 424
EcoT22I ATGCAT 1 cut(s) 639
Esp3I CGTCTC 1 cut(s) 180
FaeI CATG 6 cut(s) 241, 512, 641, 775, 821, 830
FaqI GGGAC 3 cut(s) 52, 380, 889
FatI CATG 6 cut(s) 237, 508, 637, 771, 817, 826
FauI CCCGC 1 cut(s) 329
FbaI TGATCA 1 cut(s) 823
FblI GTMKAC 1 cut(s) 98
Fnu4HI GCNGC 2 cut(s) 883, 886
FokI GGATG 2 cut(s) 218, 677
Fsp4HI GCNGC 2 cut(s) 883, 886
FspBI CTAG 3 cut(s) 137, 287, 944
GluI GCNGC 2 cut(s) 883, 886
HapII CCGG 2 cut(s) 64, 70
Hin1II CATG 6 cut(s) 241, 512, 641, 775, 821, 830
HindIII AAGCTT 1 cut(s) 30
HinfI GANTC 4 cut(s) 191, 343, 430, 592
HpaII CCGG 2 cut(s) 64, 70
HphI GGTGA 2 cut(s) 352, 918
Hpy166II GTNNAC 5 cut(s) 99, 202, 204, 439, 643
Hpy188I TCNGA 5 cut(s) 185, 257, 377, 571, 597
Hpy188III TCNNGA 9 cut(s) 324, 347, 451, 509, 545, 694, 763, 827, 871
Hpy8I GTNNAC 5 cut(s) 99, 202, 204, 439, 643
Hpy99I CGWCG 1 cut(s) 590
HpyCH4III ACNGT 1 cut(s) 484
HpyCH4V TGCA 7 cut(s) 42, 225, 241, 637, 712, 805, 888
HpyF3I CTNAG 5 cut(s) 158, 348, 678, 702, 717
Hsp92II CATG 6 cut(s) 241, 512, 641, 775, 821, 830
Ksp22I TGATCA 1 cut(s) 823
Kzo9I GATC 4 cut(s) 360, 563, 765, 823
LmnI GCTCC 1 cut(s) 133
Lsp1109I GCAGC 2 cut(s) 872, 894
MaeI CTAG 3 cut(s) 137, 287, 944
MaeIII GTNAC 1 cut(s) 383
MalI GATC 4 cut(s) 362, 565, 767, 825
MboI GATC 4 cut(s) 360, 563, 765, 823
MboII GAAGA 5 cut(s) 186, 312, 466, 531, 584
MflI RGATCY 1 cut(s) 765
MhlI GDGCHC 1 cut(s) 691
MluCI AATT 4 cut(s) 605, 650, 806, 889
MnlI CCTC 9 cut(s) 82, 124, 167, 245, 326, 349, 535, 544, 795
Mph1103I ATGCAT 1 cut(s) 639
MroXI GAANNNNTTC 1 cut(s) 31
MseI TTAA 4 cut(s) 329, 519, 753, 917
MslI CAYNNNNRTG 1 cut(s) 236
MspA1I CMGCKG 1 cut(s) 885
MspI CCGG 2 cut(s) 64, 70
MspR9I CCNGG 2 cut(s) 70, 426
MvaI CCWGG 1 cut(s) 426
NciI CCSGG 1 cut(s) 70
NdeII GATC 4 cut(s) 360, 563, 765, 823
NlaIII CATG 6 cut(s) 241, 512, 641, 775, 821, 830
NlaIV GGNNCC 3 cut(s) 68, 489, 555
NsiI ATGCAT 1 cut(s) 639
NspI RCATGY 1 cut(s) 641
PaeR7I CTCGAG 1 cut(s) 74
PagI TCATGA 2 cut(s) 508, 826
PdmI GAANNNNTTC 1 cut(s) 31
PfeI GAWTC 4 cut(s) 191, 343, 430, 592
PkrI GCNGC 2 cut(s) 884, 887
Psp6I CCWGG 1 cut(s) 424
PspGI CCWGG 1 cut(s) 424
PspN4I GGNNCC 3 cut(s) 68, 489, 555
PspPI GGNCC 1 cut(s) 66
PspXI VCTCGAGB 1 cut(s) 74
PsuI RGATCY 1 cut(s) 765
PvuII CAGCTG 1 cut(s) 885
RsaI GTAC 2 cut(s) 203, 815
RsaNI GTAC 2 cut(s) 202, 814
RseI CAYNNNNRTG 1 cut(s) 236
SaqAI TTAA 4 cut(s) 329, 519, 753, 917
SatI GCNGC 2 cut(s) 883, 886
Sau3AI GATC 4 cut(s) 360, 563, 765, 823
Sau96I GGNCC 1 cut(s) 66
ScrFI CCNGG 2 cut(s) 70, 426
SduI GDGCHC 1 cut(s) 691
SfcI CTRYAG 2 cut(s) 108, 291
Sfr274I CTCGAG 1 cut(s) 74
SinI GGWCC 1 cut(s) 66
SlaI CTCGAG 1 cut(s) 74
SmiMI CAYNNNNRTG 1 cut(s) 236
SmlI CTYRAG 4 cut(s) 74, 353, 692, 761
SmoI CTYRAG 4 cut(s) 74, 353, 692, 761
Sse9I AATT 4 cut(s) 605, 650, 806, 889
SsiI CCGC 1 cut(s) 336
SspI AATATT 2 cut(s) 460, 835
SspMI CTAG 3 cut(s) 137, 287, 944
StyD4I CCNGG 2 cut(s) 68, 424
TaaI ACNGT 1 cut(s) 484
TaqI TCGA 5 cut(s) 75, 165, 213, 855, 872
TasI AATT 4 cut(s) 605, 650, 806, 889
TatI WGTACW 1 cut(s) 201
TfiI GAWTC 4 cut(s) 191, 343, 430, 592
Tru1I TTAA 4 cut(s) 329, 519, 753, 917
Tru9I TTAA 4 cut(s) 329, 519, 753, 917
TscAI CASTG 3 cut(s) 156, 489, 691
TseI GCWGC 2 cut(s) 882, 885
TspDTI ATGAA 3 cut(s) 525, 618, 788
TspRI CASTG 3 cut(s) 156, 489, 691
VpaK11BI GGWCC 1 cut(s) 66
XceI RCATGY 1 cut(s) 641
XhoI CTCGAG 1 cut(s) 74
XmiI GTMKAC 1 cut(s) 98
XmnI GAANNNNTTC 1 cut(s) 31
XspI CTAG 3 cut(s) 137, 287, 944
Zsp2I ATGCAT 1 cut(s) 639
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.