Rw3G023390

Regulator of Vps4 activity in the MVB pathway

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0010577
Physical Location & Seq
Forward (+)
29346701 .. 29347669
969 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G023390.1

Sequence Viewer

Length: 969 bp
ATGTTGATGTTGGCCTTATTGTTGGGTTGGAGAAAAGCTTCAAAGTGCAAGAAACTACTCAAACAAGTTCGGTCCCGGCTCGCGTGCTTGAAAAACGAGAGGTCTGCTATTTCTACGCAGTTACGGAAAGAGCTTGAGAAACTAGACGACGAGGCACTGAACCTCAGGATCGAACAAGAACAACACATCAGAGACGAATCTTCGGTTTACAATTTGCTCGACCATTTCTGCAACCTCATCCTTAAGCACTCCTTTTATAATTATATTCGAACGCACAAGCAGTGTCGTCAAAGAAACCTAGCTGAAGCAGTTTCAAGTCTTGTCTTTGCTTTTTCAAGATTAAGATGCGGGGTGATTCCAGAGCTTGAGGAGATCAGGGACCTTTTTGGAGAGCGTTACGGGGAGAGTTTTGTAGAAACTACTGTGAAACTGGAGCCTGGGAATCTTGTAAACCCTACCTTCAAAAAGACTATTCTTGCTCTCTTCCCAGACTCAGTGGTGCCCAATGATAGAGTAAAACAAGAACTGGTAAAACCCTCTTTTACTAGTTCTTCTACTGATGAGGCACCAGAAAGATCATCAACAGCAGAGGAAAAGGATGACAAGGATCGCAACAATAAACTACAACTTATTACCTTCCAATCTTCTAAACATGTCCACCCCAAATTGCCTGACTGGGATGATATATTTACCAAGTTCAGAGCCCTCAAGAAGCCTAAGTACGTCAACCCTAAGTTGCCAGAATATGATGATATTACAGCCAAGGATAATGCTGTCAAGAGCCCTATTATTAATAATGAGAACACTACTGCTGATAATTACCGCTCGCAATTAGAGTACGATGATGAGCATGATAATATTGATGACGGAGCAATGTTCAGTTATTCATGGTTGTCGATAGAAAGCATTTTGGCAATGATAAACCAGCCCCAATGTGCCAGTGTCATTAACACAATCACCCCATATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
Ist1

Protein Analysis

322

Amino Acids

37.2

Weight (kDa)

6.42

Isoelectric Point (pI)

48.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ist1 PF03398 17 - 158 3.6e-21 Regulator of Vps4 activity in the MVB pathway
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 258
AccB1I GGYRCC 2 cut(s) 499, 565
AccBSI CCGCTC 1 cut(s) 825
AccII CGCG 1 cut(s) 83
AciI CCGC 2 cut(s) 348, 823
AclWI GGATC 2 cut(s) 176, 615
AcuI CTGAAG 1 cut(s) 324
AfaI GTAC 2 cut(s) 722, 839
AflII CTTAAG 1 cut(s) 242
AflIII ACRYGT 1 cut(s) 652
AgsI TTSAA 5 cut(s) 42, 91, 315, 336, 463
AhlI ACTAGT 1 cut(s) 545
AjnI CCWGG 1 cut(s) 436
AluBI AGCT 4 cut(s) 38, 133, 302, 364
AluI AGCT 4 cut(s) 38, 133, 302, 364
Alw26I GTCTC 1 cut(s) 186
AlwI GGATC 2 cut(s) 176, 615
AoxI GGCC 1 cut(s) 12
AseI ATTAAT 1 cut(s) 792
Asp700I GAANNNNTTC 1 cut(s) 37
AspS9I GGNCC 2 cut(s) 72, 379
AsuC2I CCSGG 1 cut(s) 76
AsuHPI GGTGA 2 cut(s) 364, 949
AsuII TTCGAA 1 cut(s) 268
AvaII GGWCC 2 cut(s) 72, 379
AxyI CCTNAGG 1 cut(s) 164
BaeGI GKGCMC 1 cut(s) 504
BanI GGYRCC 2 cut(s) 499, 565
BanII GRGCYC 2 cut(s) 706, 785
BarI GAAGNNNNNNTAC 2 cut(s) 704, 736
BcgI CGANNNNNNTGC 2 cut(s) 86, 120
BciT130I CCWGG 1 cut(s) 438
BcnI CCSGG 1 cut(s) 76
BcoDI GTCTC 1 cut(s) 186
BcuI ACTAGT 1 cut(s) 545
BfaI CTAG 3 cut(s) 143, 299, 546
BfrI CTTAAG 1 cut(s) 242
Bme1390I CCNGG 2 cut(s) 76, 438
Bme18I GGWCC 2 cut(s) 72, 379
BmgT120I GGNCC 2 cut(s) 72, 379
BmiI GGNNCC 5 cut(s) 74, 380, 435, 501, 567
BmrFI CCNGG 2 cut(s) 76, 438
BmrI ACTGGG 1 cut(s) 685
BmsI GCATC 1 cut(s) 335
BmuI ACTGGG 1 cut(s) 685
BpmI CTGGAG 1 cut(s) 452
Bpu14I TTCGAA 1 cut(s) 268
BpuEI CTTGAG 3 cut(s) 155, 386, 692
BpuMI CCSGG 1 cut(s) 76
BsaJI CCNNGG 2 cut(s) 437, 762
Bse1I ACTGG 4 cut(s) 435, 531, 680, 939
Bse21I CCTNAGG 1 cut(s) 164
Bse3DI GCAATG 2 cut(s) 879, 921
BseBI CCWGG 1 cut(s) 438
BseDI CCNNGG 2 cut(s) 437, 762
BseGI GGATG 3 cut(s) 237, 604, 685
BseMI GCAATG 2 cut(s) 879, 921
BseMII CTCAG 2 cut(s) 178, 507
BseNI ACTGG 4 cut(s) 435, 531, 680, 939
BseRI GAGGAG 1 cut(s) 383
BseSI GKGCMC 1 cut(s) 504
Bsh1236I CGCG 1 cut(s) 83
BshFI GGCC 1 cut(s) 14
BshNI GGYRCC 2 cut(s) 499, 565
BsiSI CCGG 1 cut(s) 76
BslFI GGGAC 2 cut(s) 58, 392
BsmAI GTCTC 1 cut(s) 186
BsmBI CGTCTC 1 cut(s) 186
BsmFI GGGAC 2 cut(s) 58, 392
BsnI GGCC 1 cut(s) 14
Bsp119I TTCGAA 1 cut(s) 268
Bsp1286I GDGCHC 3 cut(s) 504, 706, 785
Bsp143I GATC 4 cut(s) 168, 372, 575, 607
BspACI CCGC 2 cut(s) 348, 823
BspANI GGCC 1 cut(s) 14
BspCNI CTCAG 2 cut(s) 177, 506
BspFNI CGCG 1 cut(s) 83
BspLI GGNNCC 5 cut(s) 74, 380, 435, 501, 567
BspPI GGATC 2 cut(s) 176, 615
BspT104I TTCGAA 1 cut(s) 268
BspT107I GGYRCC 2 cut(s) 499, 565
BspTI CTTAAG 1 cut(s) 242
BsrBI CCGCTC 1 cut(s) 825
BsrDI GCAATG 2 cut(s) 879, 921
BsrI ACTGG 4 cut(s) 435, 531, 680, 939
BssECI CCNNGG 2 cut(s) 437, 762
BssMI GATC 4 cut(s) 168, 372, 575, 607
BssT1I CCWWGG 1 cut(s) 762
Bst2UI CCWGG 1 cut(s) 438
Bst4CI ACNGT 1 cut(s) 424
Bst6I CTCTTC 1 cut(s) 488
BstAFI CTTAAG 1 cut(s) 242
BstBI TTCGAA 1 cut(s) 268
BstC8I GCNNGC 3 cut(s) 81, 85, 827
BstDEI CTNAG 4 cut(s) 164, 493, 717, 732
BstF5I GGATG 3 cut(s) 237, 604, 685
BstFNI CGCG 1 cut(s) 83
BstKTI GATC 4 cut(s) 171, 375, 578, 610
BstMAI GTCTC 1 cut(s) 186
BstMBI GATC 4 cut(s) 168, 372, 575, 607
BstNI CCWGG 1 cut(s) 438
BstNSI RCATGY 1 cut(s) 656
BstSCI CCNGG 2 cut(s) 74, 436
BstSLI GKGCMC 1 cut(s) 504
BstUI CGCG 1 cut(s) 83
Bsu36I CCTNAGG 1 cut(s) 164
BsuRI GGCC 1 cut(s) 14
BtsCI GGATG 3 cut(s) 237, 604, 685
BtsI GCAGTG 1 cut(s) 287
BtsIMutI CAGTG 4 cut(s) 155, 287, 501, 946
Cac8I GCNNGC 3 cut(s) 81, 85, 827
Cfr13I GGNCC 2 cut(s) 72, 379
Csp6I GTAC 2 cut(s) 721, 838
CviAII CATG 3 cut(s) 653, 851, 888
CviQI GTAC 2 cut(s) 721, 838
DdeI CTNAG 4 cut(s) 164, 493, 717, 732
DpnI GATC 4 cut(s) 170, 374, 577, 609
DpnII GATC 4 cut(s) 168, 372, 575, 607
Eam1104I CTCTTC 1 cut(s) 488
EarI CTCTTC 1 cut(s) 488
Eco130I CCWWGG 1 cut(s) 762
Eco24I GRGCYC 2 cut(s) 706, 785
Eco47I GGWCC 2 cut(s) 72, 379
Eco57I CTGAAG 1 cut(s) 324
Eco81I CCTNAGG 1 cut(s) 164
EcoO109I RGGNCCY 1 cut(s) 379
EcoRII CCWGG 1 cut(s) 436
EcoT14I CCWWGG 1 cut(s) 762
EcoT38I GRGCYC 2 cut(s) 706, 785
ErhI CCWWGG 1 cut(s) 762
Esp3I CGTCTC 1 cut(s) 186
FaeI CATG 3 cut(s) 656, 854, 891
FaiI YATR 8 cut(s) 258, 264, 654, 686, 747, 852, 889, 964
FalI AAGNNNNNCTT 2 cut(s) 236, 268
FaqI GGGAC 2 cut(s) 58, 392
FatI CATG 3 cut(s) 652, 850, 887
FauI CCCGC 1 cut(s) 341
FokI GGATG 3 cut(s) 224, 611, 692
FriOI GRGCYC 2 cut(s) 706, 785
FspBI CTAG 3 cut(s) 143, 299, 546
GsuI CTGGAG 1 cut(s) 452
HaeIII GGCC 1 cut(s) 14
HapII CCGG 1 cut(s) 76
Hin1II CATG 3 cut(s) 656, 854, 891
HincII GTYRAC 1 cut(s) 727
HindII GTYRAC 1 cut(s) 727
HindIII AAGCTT 1 cut(s) 36
HinfI GANTC 4 cut(s) 197, 355, 442, 491
HpaII CCGG 1 cut(s) 76
HphI GGTGA 2 cut(s) 364, 949
Hpy166II GTNNAC 4 cut(s) 208, 451, 658, 727
Hpy188I TCNGA 2 cut(s) 191, 701
Hpy188III TCNNGA 5 cut(s) 166, 336, 359, 709, 778
Hpy8I GTNNAC 4 cut(s) 208, 451, 658, 727
Hpy99I CGWCG 1 cut(s) 152
HpyAV CCTTC 2 cut(s) 469, 646
HpyCH4III ACNGT 1 cut(s) 424
HpyCH4IV ACGT 1 cut(s) 723
HpyCH4V TGCA 2 cut(s) 48, 231
HpyF3I CTNAG 4 cut(s) 164, 493, 717, 732
HpySE526I ACGT 1 cut(s) 723
Hsp92II CATG 3 cut(s) 656, 854, 891
Kzo9I GATC 4 cut(s) 168, 372, 575, 607
LmnI GCTCC 2 cut(s) 433, 869
LweI GCATC 1 cut(s) 335
MaeI CTAG 3 cut(s) 143, 299, 546
MaeII ACGT 1 cut(s) 723
MaeIII GTNAC 2 cut(s) 120, 395
MalI GATC 4 cut(s) 170, 374, 577, 609
MbiI CCGCTC 1 cut(s) 825
MboI GATC 4 cut(s) 168, 372, 575, 607
MboII GAAGA 4 cut(s) 192, 475, 543, 636
MhlI GDGCHC 3 cut(s) 504, 706, 785
MluCI AATT 5 cut(s) 211, 259, 665, 817, 830
MlyI GAGTC 1 cut(s) 485
MmeI TCCRAC 1 cut(s) 8
MnlI CCTC 9 cut(s) 93, 145, 173, 245, 361, 547, 556, 583, 716
MroXI GAANNNNTTC 1 cut(s) 37
MseI TTAA 4 cut(s) 243, 341, 792, 948
MspCI CTTAAG 1 cut(s) 242
MspI CCGG 1 cut(s) 76
MspR9I CCNGG 2 cut(s) 76, 438
MvaI CCWGG 1 cut(s) 438
MvnI CGCG 1 cut(s) 83
NciI CCSGG 1 cut(s) 76
NdeII GATC 4 cut(s) 168, 372, 575, 607
NlaIII CATG 3 cut(s) 656, 854, 891
NlaIV GGNNCC 5 cut(s) 74, 380, 435, 501, 567
NspI RCATGY 1 cut(s) 656
NspV TTCGAA 1 cut(s) 268
PciI ACATGT 1 cut(s) 652
PdmI GAANNNNTTC 1 cut(s) 37
PfeI GAWTC 3 cut(s) 197, 355, 442
PleI GAGTC 1 cut(s) 485
PpsI GAGTC 1 cut(s) 485
PpuMI RGGWCCY 1 cut(s) 379
PscI ACATGT 1 cut(s) 652
PshBI ATTAAT 1 cut(s) 792
PsiI TTATAA 1 cut(s) 258
Psp5II RGGWCCY 1 cut(s) 379
Psp6I CCWGG 1 cut(s) 436
PspGI CCWGG 1 cut(s) 436
PspN4I GGNNCC 5 cut(s) 74, 380, 435, 501, 567
PspPI GGNCC 2 cut(s) 72, 379
PspPPI RGGWCCY 1 cut(s) 379
RsaI GTAC 2 cut(s) 722, 839
RsaNI GTAC 2 cut(s) 721, 838
SaqAI TTAA 4 cut(s) 243, 341, 792, 948
Sau3AI GATC 4 cut(s) 168, 372, 575, 607
Sau96I GGNCC 2 cut(s) 72, 379
SchI GAGTC 1 cut(s) 485
ScrFI CCNGG 2 cut(s) 76, 438
SduI GDGCHC 3 cut(s) 504, 706, 785
SfaNI GCATC 1 cut(s) 335
SfuI TTCGAA 1 cut(s) 268
SinI GGWCC 2 cut(s) 72, 379
SmlI CTYRAG 4 cut(s) 134, 242, 365, 707
SmoI CTYRAG 4 cut(s) 134, 242, 365, 707
SpeI ACTAGT 1 cut(s) 545
Sse9I AATT 5 cut(s) 211, 259, 665, 817, 830
SsiI CCGC 2 cut(s) 348, 823
SspI AATATT 1 cut(s) 859
SspMI CTAG 3 cut(s) 143, 299, 546
StyD4I CCNGG 2 cut(s) 74, 436
StyI CCWWGG 1 cut(s) 762
TaaI ACNGT 1 cut(s) 424
TaiI ACGT 1 cut(s) 726
TaqI TCGA 4 cut(s) 171, 219, 268, 896
TaqII GACCGA 1 cut(s) 60
TasI AATT 5 cut(s) 211, 259, 665, 817, 830
TfiI GAWTC 3 cut(s) 197, 355, 442
Tru1I TTAA 4 cut(s) 243, 341, 792, 948
Tru9I TTAA 4 cut(s) 243, 341, 792, 948
TscAI CASTG 4 cut(s) 162, 287, 501, 946
TspDTI ATGAA 1 cut(s) 876
TspGWI ACGGA 2 cut(s) 139, 882
TspRI CASTG 4 cut(s) 162, 287, 501, 946
Vha464I CTTAAG 1 cut(s) 242
VpaK11BI GGWCC 2 cut(s) 72, 379
VspI ATTAAT 1 cut(s) 792
XceI RCATGY 1 cut(s) 656
XmnI GAANNNNTTC 1 cut(s) 37
XspI CTAG 3 cut(s) 143, 299, 546
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.