RLG00000023236

Regulator of Vps4 activity in the MVB pathway

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0010577
Physical Location & Seq
Reverse (-)
20308223 .. 20309182
960 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000023236

Sequence Viewer

Length: 960 bp
ATGTTGATGTTGGCCTTATTGTTGGGTTGGAGAAAAGCTTCAAAGTGCAAGAAACTACTCAAACAAGTTCGGTCCCGGCTCGAGTGCTTGAAAAACGAGAGGTCTGCTATTTCTACGCAGTTACGGAAAGAGCTTGAGAAACTAGACGACGAGGCACTGAACCTCAGGATCGAACAAGAACGACACATCAGAGATGAATCTTCGGTTTACAATTTGCTCGACCATTTCTGCAACCTCATCCTTAAGCACTCCTTTTATAATTATATTCCAACGCACAAGCAGTGTCGTCAAAGAAACCTAGCTGAAGCAGTTTCAAGTCTTGTCTTTGCTTCTTCAAGATTAAGATGCGGGGTGATTCCAGAGCTTGAGGAGATCAGGGACTTATTTGGAGAGCGTTACGGGGAGAGTTTTGTAGAAACTACTGTGAAACTGGTGCCTGGGAATCTTGTAAACCCTACCTTCAAAAAGACTATTCTCGCTCTCTTCCCAGACTCAGTGGTGCCCAATGATAGTATAAAACAAGAACTGGTAAAACCCTCTTTTACTAGTTCTTCTACTGATGAGGCACCAGAAAGATCATCAACAGGAGAGGAAAAGGACGACGAGGATCGTAACAATAAACTACAACTTATTACCTTCCAATCTTCTAAACATGTCCACCCCAAATTGCCTGACTGGGATGATGTATTTACCAAGTTCAGAGCCCTCAAGAAGCCCAAGTACGTCAGCCCTAAGTTGCCAGAATATGATGATATTAGAGCCAAGTATAATGCTGTCAAGAGCCCTAATAATGAGAACACTGCTGATAATTACCGCTCGCAATTAGAATACGATGATGAGCATGATAATATTGATGACGAAGCAATGTTCAGTTATTCATGGTTGTCAATAGAGGGCATTTTGGCAATGATAAACCAGCCCCAATGTGCCAGTGTCATTAACACAATCACCCCATATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
Ist1

Protein Analysis

320

Amino Acids

36.85

Weight (kDa)

6.05

Isoelectric Point (pI)

51.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ist1 PF03398 17 - 158 4.2e-21 Regulator of Vps4 activity in the MVB pathway
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 258
AccB1I GGYRCC 3 cut(s) 433, 499, 565
AccBSI CCGCTC 1 cut(s) 816
AciI CCGC 2 cut(s) 348, 814
AclWI GGATC 2 cut(s) 176, 615
AcuI CTGAAG 1 cut(s) 324
AfaI GTAC 1 cut(s) 722
AflII CTTAAG 1 cut(s) 242
AflIII ACRYGT 1 cut(s) 652
AgsI TTSAA 5 cut(s) 42, 91, 315, 336, 463
AhlI ACTAGT 1 cut(s) 545
AjnI CCWGG 1 cut(s) 436
AluBI AGCT 4 cut(s) 38, 133, 302, 364
AluI AGCT 4 cut(s) 38, 133, 302, 364
AlwI GGATC 2 cut(s) 176, 615
Ama87I CYCGRG 1 cut(s) 80
AoxI GGCC 1 cut(s) 12
Asp700I GAANNNNTTC 1 cut(s) 37
AspS9I GGNCC 1 cut(s) 72
AsuC2I CCSGG 1 cut(s) 76
AsuHPI GGTGA 2 cut(s) 364, 940
AvaI CYCGRG 1 cut(s) 80
AvaII GGWCC 1 cut(s) 72
AxyI CCTNAGG 1 cut(s) 164
BaeGI GKGCMC 1 cut(s) 504
BanI GGYRCC 3 cut(s) 433, 499, 565
BanII GRGCYC 2 cut(s) 706, 785
BarI GAAGNNNNNNTAC 2 cut(s) 704, 736
BcgI CGANNNNNNTGC 2 cut(s) 86, 120
BciT130I CCWGG 1 cut(s) 438
BcnI CCSGG 1 cut(s) 76
BcuI ACTAGT 1 cut(s) 545
BfaI CTAG 3 cut(s) 143, 299, 546
BfrI CTTAAG 1 cut(s) 242
Bme1390I CCNGG 2 cut(s) 76, 438
Bme18I GGWCC 1 cut(s) 72
BmeT110I CYCGRG 1 cut(s) 80
BmgT120I GGNCC 1 cut(s) 72
BmiI GGNNCC 4 cut(s) 74, 435, 501, 567
BmrFI CCNGG 2 cut(s) 76, 438
BmrI ACTGGG 1 cut(s) 685
BmsI GCATC 1 cut(s) 335
BmuI ACTGGG 1 cut(s) 685
BpuEI CTTGAG 3 cut(s) 155, 386, 692
BpuMI CCSGG 1 cut(s) 76
BsaJI CCNNGG 1 cut(s) 437
Bse1I ACTGG 4 cut(s) 435, 531, 680, 930
Bse21I CCTNAGG 1 cut(s) 164
Bse3DI GCAATG 2 cut(s) 870, 912
BseBI CCWGG 1 cut(s) 438
BseDI CCNNGG 1 cut(s) 437
BseGI GGATG 2 cut(s) 237, 685
BseMI GCAATG 2 cut(s) 870, 912
BseMII CTCAG 2 cut(s) 178, 507
BseNI ACTGG 4 cut(s) 435, 531, 680, 930
BseRI GAGGAG 1 cut(s) 383
BseSI GKGCMC 1 cut(s) 504
BshFI GGCC 1 cut(s) 14
BshNI GGYRCC 3 cut(s) 433, 499, 565
BsiHKCI CYCGRG 1 cut(s) 80
BsiSI CCGG 1 cut(s) 76
BslFI GGGAC 2 cut(s) 58, 392
BsmFI GGGAC 2 cut(s) 58, 392
BsnI GGCC 1 cut(s) 14
BsoBI CYCGRG 1 cut(s) 80
Bsp1286I GDGCHC 3 cut(s) 504, 706, 785
Bsp143I GATC 4 cut(s) 168, 372, 575, 607
BspACI CCGC 2 cut(s) 348, 814
BspANI GGCC 1 cut(s) 14
BspCNI CTCAG 2 cut(s) 177, 506
BspLI GGNNCC 4 cut(s) 74, 435, 501, 567
BspPI GGATC 2 cut(s) 176, 615
BspT107I GGYRCC 3 cut(s) 433, 499, 565
BspTI CTTAAG 1 cut(s) 242
BsrBI CCGCTC 1 cut(s) 816
BsrDI GCAATG 2 cut(s) 870, 912
BsrI ACTGG 4 cut(s) 435, 531, 680, 930
BssECI CCNNGG 1 cut(s) 437
BssMI GATC 4 cut(s) 168, 372, 575, 607
Bst2UI CCWGG 1 cut(s) 438
Bst4CI ACNGT 1 cut(s) 424
Bst6I CTCTTC 1 cut(s) 488
BstAFI CTTAAG 1 cut(s) 242
BstC8I GCNNGC 1 cut(s) 818
BstDEI CTNAG 3 cut(s) 164, 493, 732
BstF5I GGATG 2 cut(s) 237, 685
BstKTI GATC 4 cut(s) 171, 375, 578, 610
BstMBI GATC 4 cut(s) 168, 372, 575, 607
BstNI CCWGG 1 cut(s) 438
BstNSI RCATGY 1 cut(s) 656
BstSCI CCNGG 2 cut(s) 74, 436
BstSLI GKGCMC 1 cut(s) 504
Bsu36I CCTNAGG 1 cut(s) 164
BsuRI GGCC 1 cut(s) 14
BtsCI GGATG 2 cut(s) 237, 685
BtsI GCAGTG 2 cut(s) 287, 798
BtsIMutI CAGTG 5 cut(s) 155, 287, 501, 798, 937
Cac8I GCNNGC 1 cut(s) 818
Cfr13I GGNCC 1 cut(s) 72
Csp6I GTAC 1 cut(s) 721
CviAII CATG 3 cut(s) 653, 842, 879
CviQI GTAC 1 cut(s) 721
DdeI CTNAG 3 cut(s) 164, 493, 732
DpnI GATC 4 cut(s) 170, 374, 577, 609
DpnII GATC 4 cut(s) 168, 372, 575, 607
Eam1104I CTCTTC 1 cut(s) 488
EarI CTCTTC 1 cut(s) 488
Eco24I GRGCYC 2 cut(s) 706, 785
Eco47I GGWCC 1 cut(s) 72
Eco57I CTGAAG 1 cut(s) 324
Eco81I CCTNAGG 1 cut(s) 164
Eco88I CYCGRG 1 cut(s) 80
EcoRII CCWGG 1 cut(s) 436
EcoT38I GRGCYC 2 cut(s) 706, 785
FaeI CATG 3 cut(s) 656, 845, 882
FaiI YATR 9 cut(s) 258, 264, 515, 654, 747, 768, 843, 880, 955
FalI AAGNNNNNCTT 2 cut(s) 236, 268
FaqI GGGAC 2 cut(s) 58, 392
FatI CATG 3 cut(s) 652, 841, 878
FauI CCCGC 1 cut(s) 341
FokI GGATG 2 cut(s) 224, 692
FriOI GRGCYC 2 cut(s) 706, 785
FspBI CTAG 3 cut(s) 143, 299, 546
HaeIII GGCC 1 cut(s) 14
HapII CCGG 1 cut(s) 76
Hin1II CATG 3 cut(s) 656, 845, 882
HindIII AAGCTT 1 cut(s) 36
HinfI GANTC 4 cut(s) 197, 355, 442, 491
HpaII CCGG 1 cut(s) 76
HphI GGTGA 2 cut(s) 364, 940
Hpy166II GTNNAC 3 cut(s) 208, 451, 658
Hpy188I TCNGA 2 cut(s) 191, 701
Hpy188III TCNNGA 5 cut(s) 166, 336, 359, 709, 778
Hpy8I GTNNAC 3 cut(s) 208, 451, 658
Hpy99I CGWCG 2 cut(s) 152, 605
HpyAV CCTTC 2 cut(s) 469, 646
HpyCH4III ACNGT 1 cut(s) 424
HpyCH4IV ACGT 1 cut(s) 723
HpyCH4V TGCA 2 cut(s) 48, 231
HpyF3I CTNAG 3 cut(s) 164, 493, 732
HpySE526I ACGT 1 cut(s) 723
Hsp92II CATG 3 cut(s) 656, 845, 882
Kzo9I GATC 4 cut(s) 168, 372, 575, 607
LweI GCATC 1 cut(s) 335
MaeI CTAG 3 cut(s) 143, 299, 546
MaeII ACGT 1 cut(s) 723
MaeIII GTNAC 3 cut(s) 120, 395, 611
MalI GATC 4 cut(s) 170, 374, 577, 609
MbiI CCGCTC 1 cut(s) 816
MboI GATC 4 cut(s) 168, 372, 575, 607
MboII GAAGA 5 cut(s) 192, 324, 475, 543, 636
MhlI GDGCHC 3 cut(s) 504, 706, 785
MluCI AATT 5 cut(s) 211, 259, 665, 808, 821
MlyI GAGTC 1 cut(s) 485
MmeI TCCRAC 2 cut(s) 8, 293
MroXI GAANNNNTTC 1 cut(s) 37
MseI TTAA 3 cut(s) 243, 341, 939
MspCI CTTAAG 1 cut(s) 242
MspI CCGG 1 cut(s) 76
MspR9I CCNGG 2 cut(s) 76, 438
MvaI CCWGG 1 cut(s) 438
NciI CCSGG 1 cut(s) 76
NdeII GATC 4 cut(s) 168, 372, 575, 607
NlaIII CATG 3 cut(s) 656, 845, 882
NlaIV GGNNCC 4 cut(s) 74, 435, 501, 567
NspI RCATGY 1 cut(s) 656
PaeR7I CTCGAG 1 cut(s) 80
PciI ACATGT 1 cut(s) 652
PdmI GAANNNNTTC 1 cut(s) 37
PfeI GAWTC 3 cut(s) 197, 355, 442
PleI GAGTC 1 cut(s) 485
PpsI GAGTC 1 cut(s) 485
PscI ACATGT 1 cut(s) 652
PsiI TTATAA 1 cut(s) 258
Psp6I CCWGG 1 cut(s) 436
PspGI CCWGG 1 cut(s) 436
PspN4I GGNNCC 4 cut(s) 74, 435, 501, 567
PspPI GGNCC 1 cut(s) 72
PspXI VCTCGAGB 1 cut(s) 80
RsaI GTAC 1 cut(s) 722
RsaNI GTAC 1 cut(s) 721
SaqAI TTAA 3 cut(s) 243, 341, 939
Sau3AI GATC 4 cut(s) 168, 372, 575, 607
Sau96I GGNCC 1 cut(s) 72
SchI GAGTC 1 cut(s) 485
ScrFI CCNGG 2 cut(s) 76, 438
SduI GDGCHC 3 cut(s) 504, 706, 785
SfaNI GCATC 1 cut(s) 335
Sfr274I CTCGAG 1 cut(s) 80
SinI GGWCC 1 cut(s) 72
SlaI CTCGAG 1 cut(s) 80
SmlI CTYRAG 5 cut(s) 80, 134, 242, 365, 707
SmoI CTYRAG 5 cut(s) 80, 134, 242, 365, 707
SpeI ACTAGT 1 cut(s) 545
Sse9I AATT 5 cut(s) 211, 259, 665, 808, 821
SsiI CCGC 2 cut(s) 348, 814
SspI AATATT 1 cut(s) 850
SspMI CTAG 3 cut(s) 143, 299, 546
StyD4I CCNGG 2 cut(s) 74, 436
TaaI ACNGT 1 cut(s) 424
TaiI ACGT 1 cut(s) 726
TaqI TCGA 3 cut(s) 81, 171, 219
TaqII GACCGA 1 cut(s) 60
TasI AATT 5 cut(s) 211, 259, 665, 808, 821
TfiI GAWTC 3 cut(s) 197, 355, 442
Tru1I TTAA 3 cut(s) 243, 341, 939
Tru9I TTAA 3 cut(s) 243, 341, 939
TscAI CASTG 5 cut(s) 162, 287, 501, 805, 937
TspDTI ATGAA 2 cut(s) 210, 867
TspGWI ACGGA 1 cut(s) 139
TspRI CASTG 5 cut(s) 162, 287, 501, 805, 937
Vha464I CTTAAG 1 cut(s) 242
VpaK11BI GGWCC 1 cut(s) 72
XceI RCATGY 1 cut(s) 656
XhoI CTCGAG 1 cut(s) 80
XmnI GAANNNNTTC 1 cut(s) 37
XspI CTAG 3 cut(s) 143, 299, 546
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.