Rh3BG296000

Regulator of Vps4 activity in the MVB pathway

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3B
OG0010577
Physical Location & Seq
Forward (+)
29713051 .. 29716564
3514 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3BG296000.1

Sequence Viewer

Length: 615 bp
ATGTTGATGTTGGCCTTATTGTTGGGTTGGAGAAAAGCTTCAAAGTGCAAGAAACTACTCAAACAAGTTCGGTCCCGTCTCGAGTGCTTGAAAAACGAGAGGTCTGCTATTTCTACGCAGTTACGGAATGAGCTTGAGAAACTAGACGACGACGCACTGAACCTCAGGATCGAACAAGAACAACACATGAGAGACGAATCTTCTGCTTTCAATTTCCTCGACCATTTCTGCAACCTCATCCTTAAGCACTCCTTTTATAATTATATTCGAACGCACAAGCAGTGTCGTCAAAACCTAGCTGAAGCAGTTTCAAGTCTTGTCTTTGCTTCTTCAAGATTAAGATGCGGGGTGATTCCAGAGCTTGAGGAGATCAGGAACTTTTTTGGAGAGTGTTACGGGGAGAGTTTTGTAGAAACTACTGTGAAACTGGTGCCTGGGAATCTTGTAAACCCTACTTTCAAAAAGACTATTCTCGCTCTCTTCCCAGACTCAGTGGTGCCCAATGATAGAGTAAAACAAGAACTGAGGCCTTGTCTCTCCTTGGCAGATCCTGACATGGCCACCATTGCAAGCTGGCAAAAAACTGGAGAAGTTGCCTTCAAGCTACGGTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
Ist1

Protein Analysis

204

Amino Acids

23.47

Weight (kDa)

8.84

Isoelectric Point (pI)

47.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ist1 PF03398 17 - 157 1e-21 Regulator of Vps4 activity in the MVB pathway
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 258
AccB1I GGYRCC 2 cut(s) 430, 496
AciI CCGC 1 cut(s) 345
AclWI GGATC 2 cut(s) 176, 542
AcoI YGGCCR 1 cut(s) 558
AcuI CTGAAG 1 cut(s) 321
AflII CTTAAG 1 cut(s) 242
AgsI TTSAA 7 cut(s) 42, 91, 211, 312, 333, 460, 601
AjnI CCWGG 1 cut(s) 433
AluBI AGCT 6 cut(s) 38, 133, 299, 361, 573, 604
AluI AGCT 6 cut(s) 38, 133, 299, 361, 573, 604
Alw26I GTCTC 3 cut(s) 83, 186, 539
AlwI GGATC 2 cut(s) 176, 542
AlwNI CAGNNNCTG 1 cut(s) 551
Ama87I CYCGRG 1 cut(s) 80
AoxI GGCC 3 cut(s) 12, 527, 558
Asp700I GAANNNNTTC 1 cut(s) 37
AspS9I GGNCC 1 cut(s) 72
AsuHPI GGTGA 1 cut(s) 361
AsuII TTCGAA 1 cut(s) 268
AvaI CYCGRG 1 cut(s) 80
AvaII GGWCC 1 cut(s) 72
AxyI CCTNAGG 1 cut(s) 164
BaeGI GKGCMC 1 cut(s) 501
BalI TGGCCA 1 cut(s) 560
BanI GGYRCC 2 cut(s) 430, 496
BcgI CGANNNNNNTGC 4 cut(s) 86, 120, 185, 219
BciT130I CCWGG 1 cut(s) 435
BcoDI GTCTC 3 cut(s) 83, 186, 539
BfaI CTAG 2 cut(s) 143, 296
BfrI CTTAAG 1 cut(s) 242
Bme1390I CCNGG 1 cut(s) 435
Bme18I GGWCC 1 cut(s) 72
BmeT110I CYCGRG 1 cut(s) 80
BmgT120I GGNCC 1 cut(s) 72
BmiI GGNNCC 3 cut(s) 74, 432, 498
BmrFI CCNGG 1 cut(s) 435
BmsI GCATC 1 cut(s) 332
BpmI CTGGAG 1 cut(s) 606
Bpu14I TTCGAA 1 cut(s) 268
BpuEI CTTGAG 2 cut(s) 155, 383
BsaJI CCNNGG 2 cut(s) 434, 540
Bse1I ACTGG 2 cut(s) 432, 589
Bse21I CCTNAGG 1 cut(s) 164
Bse3DI GCAATG 1 cut(s) 564
BseBI CCWGG 1 cut(s) 435
BseDI CCNNGG 2 cut(s) 434, 540
BseGI GGATG 1 cut(s) 237
BseMI GCAATG 1 cut(s) 564
BseMII CTCAG 3 cut(s) 178, 504, 515
BseNI ACTGG 2 cut(s) 432, 589
BseRI GAGGAG 1 cut(s) 380
BseSI GKGCMC 1 cut(s) 501
BshFI GGCC 3 cut(s) 14, 529, 560
BshNI GGYRCC 2 cut(s) 430, 496
BsiHKCI CYCGRG 1 cut(s) 80
BslFI GGGAC 1 cut(s) 58
BsmAI GTCTC 3 cut(s) 83, 186, 539
BsmBI CGTCTC 2 cut(s) 83, 186
BsmFI GGGAC 1 cut(s) 58
BsnI GGCC 3 cut(s) 14, 529, 560
BsoBI CYCGRG 1 cut(s) 80
Bsp119I TTCGAA 1 cut(s) 268
Bsp1286I GDGCHC 1 cut(s) 501
Bsp143I GATC 3 cut(s) 168, 369, 547
BspACI CCGC 1 cut(s) 345
BspANI GGCC 3 cut(s) 14, 529, 560
BspCNI CTCAG 3 cut(s) 177, 503, 516
BspLI GGNNCC 3 cut(s) 74, 432, 498
BspPI GGATC 2 cut(s) 176, 542
BspT104I TTCGAA 1 cut(s) 268
BspT107I GGYRCC 2 cut(s) 430, 496
BspTI CTTAAG 1 cut(s) 242
BsrDI GCAATG 1 cut(s) 564
BsrI ACTGG 2 cut(s) 432, 589
BssECI CCNNGG 2 cut(s) 434, 540
BssMI GATC 3 cut(s) 168, 369, 547
BssT1I CCWWGG 1 cut(s) 540
Bst2UI CCWGG 1 cut(s) 435
Bst4CI ACNGT 2 cut(s) 421, 609
Bst6I CTCTTC 1 cut(s) 485
BstAFI CTTAAG 1 cut(s) 242
BstBI TTCGAA 1 cut(s) 268
BstC8I GCNNGC 2 cut(s) 571, 575
BstDEI CTNAG 3 cut(s) 164, 490, 524
BstF5I GGATG 1 cut(s) 237
BstKTI GATC 3 cut(s) 171, 372, 550
BstMAI GTCTC 3 cut(s) 83, 186, 539
BstMBI GATC 3 cut(s) 168, 369, 547
BstMWI GCNNNNNNNGC 1 cut(s) 566
BstNI CCWGG 1 cut(s) 435
BstSCI CCNGG 1 cut(s) 433
BstSLI GKGCMC 1 cut(s) 501
BstX2I RGATCY 1 cut(s) 547
BstYI RGATCY 1 cut(s) 547
Bsu36I CCTNAGG 1 cut(s) 164
BsuRI GGCC 3 cut(s) 14, 529, 560
BtsCI GGATG 1 cut(s) 237
BtsI GCAGTG 1 cut(s) 287
BtsIMutI CAGTG 3 cut(s) 155, 287, 498
Cac8I GCNNGC 2 cut(s) 571, 575
CaiI CAGNNNCTG 1 cut(s) 551
Cfr13I GGNCC 1 cut(s) 72
CseI GACGC 1 cut(s) 161
CviAII CATG 2 cut(s) 187, 556
CviJI RGCY 9 cut(s) 14, 38, 133, 299, 361, 529, 560, 573, 604
CviKI_1 RGCY 9 cut(s) 14, 38, 133, 299, 361, 529, 560, 573, 604
DdeI CTNAG 3 cut(s) 164, 490, 524
DpnI GATC 3 cut(s) 170, 371, 549
DpnII GATC 3 cut(s) 168, 369, 547
EaeI YGGCCR 1 cut(s) 558
Eam1104I CTCTTC 1 cut(s) 485
EarI CTCTTC 1 cut(s) 485
Eco130I CCWWGG 1 cut(s) 540
Eco147I AGGCCT 1 cut(s) 529
Eco47I GGWCC 1 cut(s) 72
Eco57I CTGAAG 1 cut(s) 321
Eco81I CCTNAGG 1 cut(s) 164
Eco88I CYCGRG 1 cut(s) 80
EcoRII CCWGG 1 cut(s) 433
EcoT14I CCWWGG 1 cut(s) 540
ErhI CCWWGG 1 cut(s) 540
Esp3I CGTCTC 2 cut(s) 83, 186
FaeI CATG 2 cut(s) 190, 559
FaiI YATR 4 cut(s) 188, 258, 264, 557
FalI AAGNNNNNCTT 2 cut(s) 236, 268
FaqI GGGAC 1 cut(s) 58
FatI CATG 2 cut(s) 186, 555
FauI CCCGC 1 cut(s) 338
FokI GGATG 1 cut(s) 224
FspBI CTAG 2 cut(s) 143, 296
GsuI CTGGAG 1 cut(s) 606
HaeIII GGCC 3 cut(s) 14, 529, 560
HgaI GACGC 1 cut(s) 161
Hin1II CATG 2 cut(s) 190, 559
HindIII AAGCTT 1 cut(s) 36
HinfI GANTC 4 cut(s) 197, 352, 439, 488
HphI GGTGA 1 cut(s) 361
Hpy166II GTNNAC 1 cut(s) 448
Hpy188III TCNNGA 6 cut(s) 80, 166, 333, 356, 373, 551
Hpy8I GTNNAC 1 cut(s) 448
Hpy99I CGWCG 2 cut(s) 152, 155
HpyAV CCTTC 1 cut(s) 607
HpyCH4III ACNGT 2 cut(s) 421, 609
HpyCH4V TGCA 3 cut(s) 48, 231, 569
HpyF10VI GCNNNNNNNGC 1 cut(s) 566
HpyF3I CTNAG 3 cut(s) 164, 490, 524
Hsp92II CATG 2 cut(s) 190, 559
Kzo9I GATC 3 cut(s) 168, 369, 547
LweI GCATC 1 cut(s) 332
MaeI CTAG 2 cut(s) 143, 296
MaeIII GTNAC 2 cut(s) 120, 392
MalI GATC 3 cut(s) 170, 371, 549
MboI GATC 3 cut(s) 168, 369, 547
MboII GAAGA 3 cut(s) 192, 321, 472
MflI RGATCY 1 cut(s) 547
MhlI GDGCHC 1 cut(s) 501
MlsI TGGCCA 1 cut(s) 560
MluCI AATT 2 cut(s) 211, 259
MluNI TGGCCA 1 cut(s) 560
MlyI GAGTC 1 cut(s) 482
MmeI TCCRAC 1 cut(s) 8
MnlI CCTC 6 cut(s) 93, 173, 227, 245, 358, 519
Mox20I TGGCCA 1 cut(s) 560
MroXI GAANNNNTTC 1 cut(s) 37
MscI TGGCCA 1 cut(s) 560
MseI TTAA 3 cut(s) 243, 338, 613
Msp20I TGGCCA 1 cut(s) 560
MspCI CTTAAG 1 cut(s) 242
MspR9I CCNGG 1 cut(s) 435
MvaI CCWGG 1 cut(s) 435
MwoI GCNNNNNNNGC 1 cut(s) 566
NdeII GATC 3 cut(s) 168, 369, 547
NlaIII CATG 2 cut(s) 190, 559
NlaIV GGNNCC 3 cut(s) 74, 432, 498
NspV TTCGAA 1 cut(s) 268
PaeR7I CTCGAG 1 cut(s) 80
PceI AGGCCT 1 cut(s) 529
PdmI GAANNNNTTC 1 cut(s) 37
PfeI GAWTC 3 cut(s) 197, 352, 439
PleI GAGTC 1 cut(s) 482
PpsI GAGTC 1 cut(s) 482
PsiI TTATAA 1 cut(s) 258
Psp6I CCWGG 1 cut(s) 433
PspGI CCWGG 1 cut(s) 433
PspN4I GGNNCC 3 cut(s) 74, 432, 498
PspPI GGNCC 1 cut(s) 72
PstNI CAGNNNCTG 1 cut(s) 551
PsuI RGATCY 1 cut(s) 547
SaqAI TTAA 3 cut(s) 243, 338, 613
Sau3AI GATC 3 cut(s) 168, 369, 547
Sau96I GGNCC 1 cut(s) 72
SchI GAGTC 1 cut(s) 482
ScrFI CCNGG 1 cut(s) 435
SduI GDGCHC 1 cut(s) 501
SfaNI GCATC 1 cut(s) 332
Sfr274I CTCGAG 1 cut(s) 80
SfuI TTCGAA 1 cut(s) 268
SinI GGWCC 1 cut(s) 72
SlaI CTCGAG 1 cut(s) 80
SmlI CTYRAG 4 cut(s) 80, 134, 242, 362
SmoI CTYRAG 4 cut(s) 80, 134, 242, 362
Sse9I AATT 2 cut(s) 211, 259
SseBI AGGCCT 1 cut(s) 529
SsiI CCGC 1 cut(s) 345
SspMI CTAG 2 cut(s) 143, 296
StuI AGGCCT 1 cut(s) 529
StyD4I CCNGG 1 cut(s) 433
StyI CCWWGG 1 cut(s) 540
TaaI ACNGT 2 cut(s) 421, 609
TaqI TCGA 4 cut(s) 81, 171, 219, 268
TaqII GACCGA 1 cut(s) 60
TasI AATT 2 cut(s) 211, 259
TfiI GAWTC 3 cut(s) 197, 352, 439
Tru1I TTAA 3 cut(s) 243, 338, 613
Tru9I TTAA 3 cut(s) 243, 338, 613
TscAI CASTG 3 cut(s) 162, 287, 498
TspGWI ACGGA 1 cut(s) 139
TspRI CASTG 3 cut(s) 162, 287, 498
Vha464I CTTAAG 1 cut(s) 242
VpaK11BI GGWCC 1 cut(s) 72
XhoI CTCGAG 1 cut(s) 80
XmnI GAANNNNTTC 1 cut(s) 37
XspI CTAG 2 cut(s) 143, 296
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.