FvH4_6g10940

Belongs to the GRAS family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0004862
Physical Location & Seq
Reverse (-)
6548671 .. 6550230
1560 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g10940.t1

Sequence Viewer

Length: 1560 bp
ATGGTAAACTCATCTTTTGATCAATCTGATCTCATGAACATTGTTGATTCTCAAATAGGCAGCTCAGATTTTTCTTCCTTGGTCCTCAACTCAGATGTATTTTCTGACTTATCCGAAACTTGTTCATACCCTCTTGCCACCGTGTTTTGTGGTGATGATCTGATCCAGGTCTCATCACAGCTGGAGGAATTTCCAATGAATTTGGAAGAAATTGACCATGATTTGATGATAGAATGTTTGTATGGTAATTTCTTGGAAAACAATGGAGGGACTTTACTTTCACAAGAAGTTTCCACACGATTTTCTATTGAAGGAGATTGTTTGTCAGATTCATTGGTGACTTGTGAAGTATCATCAATGGACTCAACATCAATCCTAACATCACAGACTGCGCCACCAGAATTGGTGGACATAGATAACGAATCAGGCCTTCGTCATCTTCTCAAGGCTTATGCAGAATCCATGGAGAATGGACAGAAGGAGCTAGAGGAGGTAATTTTCAGATGCATCAAGGACAAAGCCAGCCCAATTGGACAGACTTTGGATCGTCTTGCATTTAACTTGTGCCGAGAAGCTGTTGATGATCAACAAGGCGATGTTGATTATCTGAAACAACAATCCTACAAGAATTTTGAGCCTGCATTTGAGTTGTTTTACCAAAACTTTCCCTATGGGAAGTTTGCTCACTATGCGGCAAACTCAGCAATCCTAGAGGCTATTCCTGAGGATACAGAGATGGTTCACATAGTAGATTTCGATATGGGAGAAGGGCTGCAGTTGTCACAGATGATTGAAGCTTTAGAACAAAGACTCAAAACACTTAAGGTGACAGCAATTAAATGGGAAGATGAAGAGAGTGGTTGTGCTCTACAGGGCACATTTGGCGAGAGGAAGAAGCAACTTCAGAAACATGCAAGATCTTTTGGTCTAAACTTGAAGGTGGAGGAAGTGGCTCTAGAAGATCTGGTGGCAGAGATAAAGAAGACAAAAAAGAGGGCAGTAAGAGAATTCTTAGCCTTTAATTGTATGGTTTCGCTTCCACACATGCGGAGGAGGAGAAGTAGAAGTCTCGTCATAGAGTTTCTAAGACTGGCTAAGGATTTGTTAGCCACATCTGGGAACTACAAGACTGGAAAAACAGGAATTGTGACCTTTGGTGATGGGGAGGCATGTGAGAGACTCGGGAATCAGTTGGATTTCAGCTCGTTCTTTGATGGGAATCTGGTGCATTACCAAGCCTTATCAGAGTCAATAGAGTCAAATTTCCCGAGTCATCTTGCAGAAGCAAGAATGGTGCTGGAAAACATCTTCCTAGCACCATACGTCTCTCCTCAGACTTGGTTCCAAAACTGGTCCGAAATGAAGGAAAACAGCCATGTTCAACCATGGCTTGGGCTGGAGGGTCGTAAATTGAGCACAGAGAACTTGTTGGAAGCCAAGGAAATAGTTGGAGACGAAAGCTCCTATGGAATTAAGATTGGAGGAAAGAATGGAAATGAAATGACCTTAGAATGGAGAGAGAATCCTTTGGTCCGAGTTTCTACCTGGGAAAAACAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GRAS

Protein Analysis

520

Amino Acids

58.54

Weight (kDa)

4.64

Isoelectric Point (pI)

48.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRAS PF03514 144 - 517 5.4e-40 GRAS domain family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1391
AciI CCGC 2 cut(s) 692, 1048
AclWI GGATC 2 cut(s) 157, 552
AcsI RAATTY 5 cut(s) 188, 199, 628, 1007, 1261
AcuI CTGAAG 1 cut(s) 887
AfiI CCNNNNNNNGG 3 cut(s) 1116, 1391, 1512
AflII CTTAAG 1 cut(s) 821
AgsI TTSAA 4 cut(s) 311, 794, 937, 1382
AjnI CCWGG 2 cut(s) 165, 1544
AluBI AGCT 7 cut(s) 63, 181, 484, 575, 797, 1203, 1461
AluI AGCT 7 cut(s) 63, 181, 484, 575, 797, 1203, 1461
Alw21I GWGCWC 2 cut(s) 868, 1418
Alw26I GTCTC 5 cut(s) 175, 1073, 1171, 1330, 1446
AlwI GGATC 2 cut(s) 157, 552
Ama87I CYCGRG 2 cut(s) 1181, 1267
AoxI GGCC 1 cut(s) 427
ApeKI GCWGC 2 cut(s) 60, 772
ApoI RAATTY 5 cut(s) 188, 199, 628, 1007, 1261
AspLEI GCGC 1 cut(s) 394
AspS9I GGNCC 3 cut(s) 82, 1353, 1531
AsuHPI GGTGA 4 cut(s) 164, 349, 838, 1169
AvaI CYCGRG 2 cut(s) 1181, 1267
AvaII GGWCC 3 cut(s) 82, 1353, 1531
AxyI CCTNAGG 1 cut(s) 723
BaeGI GKGCMC 1 cut(s) 878
BbsI GAAGAC 1 cut(s) 989
Bbv12I GWGCWC 2 cut(s) 868, 1418
BbvI GCAGC 2 cut(s) 72, 759
BccI CCATC 3 cut(s) 730, 1154, 1208
BciT130I CCWGG 2 cut(s) 167, 1546
BciVI GTATCC 1 cut(s) 721
BclI TGATCA 2 cut(s) 19, 583
BcoDI GTCTC 5 cut(s) 175, 1073, 1171, 1330, 1446
BfaI CTAG 4 cut(s) 485, 710, 956, 1313
BfmI CTRYAG 2 cut(s) 773, 869
BfrI CTTAAG 1 cut(s) 821
BfuI GTATCC 1 cut(s) 721
BglII AGATCT 2 cut(s) 917, 961
BisI GCNGC 3 cut(s) 61, 693, 773
BlsI GCNGC 3 cut(s) 62, 694, 774
Bme1390I CCNGG 2 cut(s) 167, 1546
Bme18I GGWCC 3 cut(s) 82, 1353, 1531
BmeT110I CYCGRG 2 cut(s) 1181, 1267
BmgT120I GGNCC 3 cut(s) 82, 1353, 1531
BmiI GGNNCC 1 cut(s) 1343
BmrFI CCNGG 2 cut(s) 167, 1546
BmsI GCATC 2 cut(s) 494, 516
BpiI GAAGAC 1 cut(s) 989
BpmI CTGGAG 2 cut(s) 203, 1418
Bpu10I CCTNAGC 1 cut(s) 1095
BpuEI CTTGAG 1 cut(s) 428
BsaBI GATNNNNATC 1 cut(s) 1218
BsaI GGTCTC 1 cut(s) 175
BsaJI CCNNGG 5 cut(s) 78, 462, 1385, 1437, 1545
BsaXI ACNNNNNCTCC 4 cut(s) 1045, 1075, 1445, 1475
Bsc4I CCNNNNNNNGG 3 cut(s) 1116, 1391, 1512
Bse1I ACTGG 3 cut(s) 1095, 1135, 1355
Bse21I CCTNAGG 1 cut(s) 723
Bse8I GATNNNNATC 1 cut(s) 1218
BseBI CCWGG 2 cut(s) 167, 1546
BseDI CCNNGG 5 cut(s) 78, 462, 1385, 1437, 1545
BseJI GATNNNNATC 1 cut(s) 1218
BseLI CCNNNNNNNGG 3 cut(s) 1116, 1391, 1512
BseMII CTCAG 5 cut(s) 78, 105, 714, 714, 1346
BseNI ACTGG 3 cut(s) 1095, 1135, 1355
BseRI GAGGAG 4 cut(s) 503, 1066, 1069, 1320
BseSI GKGCMC 1 cut(s) 878
BseXI GCAGC 2 cut(s) 72, 759
BshFI GGCC 1 cut(s) 429
BsiHKAI GWGCWC 2 cut(s) 868, 1418
BsiHKCI CYCGRG 2 cut(s) 1181, 1267
BslFI GGGAC 1 cut(s) 283
BslI CCNNNNNNNGG 3 cut(s) 1116, 1391, 1512
BsmAI GTCTC 5 cut(s) 175, 1073, 1171, 1330, 1446
BsmBI CGTCTC 2 cut(s) 1330, 1446
BsmFI GGGAC 1 cut(s) 283
BsnI GGCC 1 cut(s) 429
Bso31I GGTCTC 1 cut(s) 175
BsoBI CYCGRG 2 cut(s) 1181, 1267
Bsp1286I GDGCHC 3 cut(s) 868, 878, 1418
Bsp143I GATC 8 cut(s) 19, 28, 157, 162, 544, 583, 917, 961
Bsp19I CCATGG 2 cut(s) 462, 1385
BspACI CCGC 2 cut(s) 692, 1048
BspANI GGCC 1 cut(s) 429
BspCNI CTCAG 5 cut(s) 77, 104, 713, 715, 1345
BspHI TCATGA 1 cut(s) 33
BspLI GGNNCC 1 cut(s) 1343
BspMAI CTGCAG 1 cut(s) 777
BspPI GGATC 2 cut(s) 157, 552
BspTI CTTAAG 1 cut(s) 821
BspTNI GGTCTC 1 cut(s) 175
BsrI ACTGG 3 cut(s) 1095, 1135, 1355
BssECI CCNNGG 5 cut(s) 78, 462, 1385, 1437, 1545
BssMI GATC 8 cut(s) 19, 28, 157, 162, 544, 583, 917, 961
BssT1I CCWWGG 4 cut(s) 78, 462, 1385, 1437
Bst2UI CCWGG 2 cut(s) 167, 1546
Bst4CI ACNGT 1 cut(s) 142
Bst6I CTCTTC 1 cut(s) 846
BstAFI CTTAAG 1 cut(s) 821
BstC8I GCNNGC 2 cut(s) 523, 639
BstDEI CTNAG 9 cut(s) 64, 91, 700, 723, 1012, 1085, 1095, 1332, 1507
BstDSI CCRYGG 2 cut(s) 462, 1385
BstHHI GCGC 1 cut(s) 394
BstKTI GATC 8 cut(s) 22, 31, 160, 165, 547, 586, 920, 964
BstMAI GTCTC 5 cut(s) 175, 1073, 1171, 1330, 1446
BstMBI GATC 8 cut(s) 19, 28, 157, 162, 544, 583, 917, 961
BstMWI GCNNNNNNNGC 3 cut(s) 689, 701, 882
BstNI CCWGG 2 cut(s) 167, 1546
BstNSI RCATGY 3 cut(s) 914, 1048, 1173
BstSCI CCNGG 2 cut(s) 165, 1544
BstSFI CTRYAG 2 cut(s) 773, 869
BstSLI GKGCMC 1 cut(s) 878
BstV1I GCAGC 2 cut(s) 72, 759
BstV2I GAAGAC 1 cut(s) 989
BstX2I RGATCY 2 cut(s) 917, 961
BstYI RGATCY 2 cut(s) 917, 961
Bsu36I CCTNAGG 1 cut(s) 723
BsuI GTATCC 1 cut(s) 721
BsuRI GGCC 1 cut(s) 429
BtgI CCRYGG 2 cut(s) 462, 1385
BtgZI GCGATG 1 cut(s) 609
Cac8I GCNNGC 2 cut(s) 523, 639
CciI TCATGA 1 cut(s) 33
CfoI GCGC 1 cut(s) 394
Cfr13I GGNCC 3 cut(s) 82, 1353, 1531
CspCI CAANNNNNGTGG 2 cut(s) 384, 419
CviAII CATG 8 cut(s) 34, 218, 463, 911, 1045, 1170, 1376, 1386
DdeI CTNAG 9 cut(s) 64, 91, 700, 723, 1012, 1085, 1095, 1332, 1507
DpnI GATC 8 cut(s) 21, 30, 159, 164, 546, 585, 919, 963
DpnII GATC 8 cut(s) 19, 28, 157, 162, 544, 583, 917, 961
Eam1104I CTCTTC 1 cut(s) 846
EarI CTCTTC 1 cut(s) 846
Eco130I CCWWGG 4 cut(s) 78, 462, 1385, 1437
Eco147I AGGCCT 1 cut(s) 429
Eco31I GGTCTC 1 cut(s) 175
Eco47I GGWCC 3 cut(s) 82, 1353, 1531
Eco57I CTGAAG 1 cut(s) 887
Eco81I CCTNAGG 1 cut(s) 723
Eco88I CYCGRG 2 cut(s) 1181, 1267
EcoRI GAATTC 1 cut(s) 1007
EcoRII CCWGG 2 cut(s) 165, 1544
EcoT14I CCWWGG 4 cut(s) 78, 462, 1385, 1437
EcoT22I ATGCAT 1 cut(s) 509
ErhI CCWWGG 4 cut(s) 78, 462, 1385, 1437
Esp3I CGTCTC 2 cut(s) 1330, 1446
FaeI CATG 8 cut(s) 37, 221, 466, 914, 1048, 1173, 1379, 1389
FaqI GGGAC 1 cut(s) 283
FatI CATG 8 cut(s) 33, 217, 462, 910, 1044, 1169, 1375, 1385
FbaI TGATCA 2 cut(s) 19, 583
Fnu4HI GCNGC 3 cut(s) 61, 693, 773
Fsp4HI GCNGC 3 cut(s) 61, 693, 773
FspBI CTAG 4 cut(s) 485, 710, 956, 1313
GlaI GCGC 1 cut(s) 393
GluI GCNGC 3 cut(s) 61, 693, 773
GsuI CTGGAG 2 cut(s) 203, 1418
HaeIII GGCC 1 cut(s) 429
HhaI GCGC 1 cut(s) 394
Hin1II CATG 8 cut(s) 37, 221, 466, 914, 1048, 1173, 1379, 1389
Hin6I GCGC 1 cut(s) 392
HinP1I GCGC 1 cut(s) 392
HindIII AAGCTT 1 cut(s) 795
HphI GGTGA 4 cut(s) 164, 349, 838, 1169
Hpy166II GTNNAC 3 cut(s) 7, 409, 742
Hpy188III TCNNGA 5 cut(s) 34, 722, 956, 1183, 1267
Hpy8I GTNNAC 3 cut(s) 7, 409, 742
HpyAV CCTTC 6 cut(s) 305, 440, 472, 761, 931, 1357
HpyCH4III ACNGT 1 cut(s) 142
HpyCH4IV ACGT 1 cut(s) 1323
HpyCH4V TGCA 8 cut(s) 455, 507, 554, 641, 775, 914, 1228, 1280
HpyF10VI GCNNNNNNNGC 3 cut(s) 689, 701, 882
HpyF3I CTNAG 9 cut(s) 64, 91, 700, 723, 1012, 1085, 1095, 1332, 1507
HpySE526I ACGT 1 cut(s) 1323
Hsp92II CATG 8 cut(s) 37, 221, 466, 914, 1048, 1173, 1379, 1389
HspAI GCGC 1 cut(s) 392
Ksp22I TGATCA 2 cut(s) 19, 583
Kzo9I GATC 8 cut(s) 19, 28, 157, 162, 544, 583, 917, 961
LmnI GCTCC 2 cut(s) 481, 1466
Lsp1109I GCAGC 2 cut(s) 72, 759
LweI GCATC 2 cut(s) 494, 516
MaeI CTAG 4 cut(s) 485, 710, 956, 1313
MaeII ACGT 1 cut(s) 1323
MaeIII GTNAC 4 cut(s) 337, 780, 826, 1147
MalI GATC 8 cut(s) 21, 30, 159, 164, 546, 585, 919, 963
MboI GATC 8 cut(s) 19, 28, 157, 162, 544, 583, 917, 961
MboII GAAGA 9 cut(s) 66, 218, 431, 857, 863, 904, 971, 994, 1300
MfeI CAATTG 1 cut(s) 528
MflI RGATCY 2 cut(s) 917, 961
MhlI GDGCHC 3 cut(s) 868, 878, 1418
MlyI GAGTC 6 cut(s) 356, 804, 1173, 1256, 1265, 1279
MmeI TCCRAC 3 cut(s) 1173, 1410, 1429
Mph1103I ATGCAT 1 cut(s) 509
MseI TTAA 5 cut(s) 558, 822, 837, 1020, 1473
MspA1I CMGCKG 1 cut(s) 181
MspCI CTTAAG 1 cut(s) 821
MspR9I CCNGG 2 cut(s) 167, 1546
MunI CAATTG 1 cut(s) 528
MvaI CCWGG 2 cut(s) 167, 1546
MwoI GCNNNNNNNGC 3 cut(s) 689, 701, 882
NcoI CCATGG 2 cut(s) 462, 1385
NdeII GATC 8 cut(s) 19, 28, 157, 162, 544, 583, 917, 961
NlaIII CATG 8 cut(s) 37, 221, 466, 914, 1048, 1173, 1379, 1389
NlaIV GGNNCC 1 cut(s) 1343
NmeAIII GCCGAG 1 cut(s) 593
NmuCI GTSAC 4 cut(s) 337, 780, 826, 1147
NsiI ATGCAT 1 cut(s) 509
NspI RCATGY 3 cut(s) 914, 1048, 1173
PagI TCATGA 1 cut(s) 33
PceI AGGCCT 1 cut(s) 429
PfeI GAWTC 7 cut(s) 47, 329, 422, 458, 1186, 1219, 1522
PflMI CCANNNNNTGG 1 cut(s) 1391
PkrI GCNGC 3 cut(s) 62, 694, 774
PleI GAGTC 6 cut(s) 356, 804, 1173, 1255, 1264, 1278
PpsI GAGTC 6 cut(s) 356, 804, 1173, 1255, 1264, 1278
Psp6I CCWGG 2 cut(s) 165, 1544
PspGI CCWGG 2 cut(s) 165, 1544
PspN4I GGNNCC 1 cut(s) 1343
PspPI GGNCC 3 cut(s) 82, 1353, 1531
PstI CTGCAG 1 cut(s) 777
PsuI RGATCY 2 cut(s) 917, 961
PvuII CAGCTG 1 cut(s) 181
SaqAI TTAA 5 cut(s) 558, 822, 837, 1020, 1473
SatI GCNGC 3 cut(s) 61, 693, 773
Sau3AI GATC 8 cut(s) 19, 28, 157, 162, 544, 583, 917, 961
Sau96I GGNCC 3 cut(s) 82, 1353, 1531
SchI GAGTC 6 cut(s) 356, 804, 1173, 1256, 1265, 1279
ScrFI CCNGG 2 cut(s) 167, 1546
SduI GDGCHC 3 cut(s) 868, 878, 1418
SfaNI GCATC 2 cut(s) 494, 516
SfcI CTRYAG 2 cut(s) 773, 869
SinI GGWCC 3 cut(s) 82, 1353, 1531
SmlI CTYRAG 2 cut(s) 443, 821
SmoI CTYRAG 2 cut(s) 443, 821
SseBI AGGCCT 1 cut(s) 429
SsiI CCGC 2 cut(s) 692, 1048
SspMI CTAG 4 cut(s) 485, 710, 956, 1313
StuI AGGCCT 1 cut(s) 429
StyD4I CCNGG 2 cut(s) 165, 1544
StyI CCWWGG 4 cut(s) 78, 462, 1385, 1437
TaaI ACNGT 1 cut(s) 142
TaiI ACGT 1 cut(s) 1326
TaqI TCGA 1 cut(s) 756
TauI GCSGC 1 cut(s) 695
TfiI GAWTC 7 cut(s) 47, 329, 422, 458, 1186, 1219, 1522
Tru1I TTAA 5 cut(s) 558, 822, 837, 1020, 1473
Tru9I TTAA 5 cut(s) 558, 822, 837, 1020, 1473
TseFI GTSAC 4 cut(s) 337, 780, 826, 1147
TseI GCWGC 2 cut(s) 60, 772
Tsp45I GTSAC 4 cut(s) 337, 780, 826, 1147
TspDTI ATGAA 7 cut(s) 50, 114, 212, 321, 864, 1376, 1512
Van91I CCANNNNNTGG 1 cut(s) 1391
Vha464I CTTAAG 1 cut(s) 821
VpaK11BI GGWCC 3 cut(s) 82, 1353, 1531
XapI RAATTY 5 cut(s) 188, 199, 628, 1007, 1261
XbaI TCTAGA 1 cut(s) 955
XceI RCATGY 3 cut(s) 914, 1048, 1173
XspI CTAG 4 cut(s) 485, 710, 956, 1313
Zsp2I ATGCAT 1 cut(s) 509
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.