Rw3G009600

Belongs to the GRAS family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0004862
Physical Location & Seq
Reverse (-)
9183570 .. 9185424
1855 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G009600.1

Sequence Viewer

Length: 1530 bp
ATGAACATGGATGATAATCAAATGGGTAGCTCAGATTTTTCTTCTTTGATCATCAACTCAGATGAATTTTCTGACTTATCCGACACTTGTTCAGACCCTTTTGCCTCCTTGTTTTCTGGTAATGATCTGATTCAGGTCTCATCACAGGTGGAGGTATTCCCAATGAATTTGGAAGAATTTGACCCTAATTTGATGATAGACTGTTTGTATGGTAGTTTCTTGGAAGGGAGTGAAGGGAGTCTTCCTTCACAAGAAATCTCCTCACAATTTTCTATTGAAGGTGATTGTGTGTCAAATTCATTGGTGACTTCTGAAGTATCATCAATGGACTCGACATCAATCCAAACATCACTAACTTTGCCTGCAGAAGTGGTGGAAATAGATAACGAATCAAGTATTCGTCATCTTCTCAAGGCTCATGCAGAATCCATGGCAAATAGACAGAAAGAGCTAGAGGAGGTAATTTTCAGATGCATCAAGGACAAAGTCAGCCCAATTGGGCAGAGTTTGGATCGTCTTGCATTTCACTTGTGTCGAGAAGTTGTTGATGATCAACAAGGCGATGTTGATTATCTGAAACAACAATCCTACAAGAATTTTGAGCCTGCATTCAAGTTGTTCTACCAAAACTTTCCCTACGGGAAGTTTGCTCACTATGCGGCAAACTCAGCAATCCTAGAGTCTATTCCCGAGGATACAGAGATGGTTCACGTAGTAGATTTTGATATGGGAGAAGGGGTGCAGTTGTCACAGATGATTGAAGCTTTAGCACAAAGACTCAAAACATTGAGAGTGACAGCAATTAAATGGGAAGATGAAGAAAGTGATTGTGTTCTACAAGGAAGATTTGACGAGAGAAAGAAGCAACTTCAGAATCATGCAAGATCTTTTGGCTTAAACTTGAAGGTGGAGGAAGTTGCACTAGAGGATCTGGTGGGAGAGATAAAGAAGACAAAAAAGAGGGGTGTAAAAGAATTCTTAGCCTTCAACTGTATGGTCTCGCTTCCACACATGCGGAGGAGGAGAAGCCGAAGTCTTGCATTGGAATTTCTGAGACTGGCTAAGGATTTGTTGGCCAAATCAGGGAACTACAGGACTGGGAAAACAGGCATTGTGACCTTTGGAGATGGGGAGGCTTGTGGGAGACTTGAAAATCAGTTGGATTTCAGCTCTTTCTTTGATGGGAATCTGGTGCATTACCAAGCCTTAACAGAGTCAATGGAATCAAATTTTCCAAGTCATCTAGCAGAAGCAAGAATGGTGATGGAAAGCATTTTCCTAGCACCATATGTATCGTCTCAGGCTTGGTTCCGGAACTGGACCGAAATGAGGGAAGACTGCCATGTACAACCATGGTTTGGGCTGGAGGGTCGTAGACTGAGCAAAGAGAACTTGTTGGAAGCTAAGGGCATTGTTGGAGACGAAAGCTCCTATGGAATTAGGATTGGAGGAGAGAATGGAAATGAGATGGCCTTGGAATGGAGAGGGAATCCTTTGGTTAGAGTTTCAACCTGGGAAAACCAAAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GRAS

Protein Analysis

509

Amino Acids

57.52

Weight (kDa)

4.75

Isoelectric Point (pI)

43.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRAS PF03514 134 - 506 4.8e-41 GRAS domain family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1358
AccI GTMKAC 1 cut(s) 1375
AccIII TCCGGA 1 cut(s) 1311
AciI CCGC 2 cut(s) 659, 1015
AclWI GGATC 2 cut(s) 519, 936
AcoI YGGCCR 1 cut(s) 1074
AcsI RAATTY 8 cut(s) 65, 166, 176, 295, 595, 974, 1046, 1228
AcuI CTGAAG 2 cut(s) 333, 854
AfaI GTAC 1 cut(s) 1347
AfiI CCNNNNNNNGG 4 cut(s) 1083, 1329, 1358, 1479
AgsI TTSAA 7 cut(s) 278, 613, 761, 904, 988, 1151, 1509
AjnI CCWGG 1 cut(s) 1511
AluBI AGCT 7 cut(s) 30, 451, 764, 1170, 1403, 1428, 1527
AluI AGCT 7 cut(s) 30, 451, 764, 1170, 1403, 1428, 1527
Alw26I GTCTC 6 cut(s) 142, 1003, 1048, 1138, 1302, 1413
AlwI GGATC 2 cut(s) 519, 936
Ama87I CYCGRG 1 cut(s) 689
Aor13HI TCCGGA 1 cut(s) 1311
AoxI GGCC 2 cut(s) 1074, 1470
ApoI RAATTY 8 cut(s) 65, 166, 176, 295, 595, 974, 1046, 1228
AspS9I GGNCC 1 cut(s) 1320
AsuHPI GGTGA 3 cut(s) 293, 316, 1273
AvaI CYCGRG 1 cut(s) 689
AvaII GGWCC 1 cut(s) 1320
BalI TGGCCA 1 cut(s) 1076
BbsI GAAGAC 3 cut(s) 233, 956, 1341
BccI CCATC 5 cut(s) 697, 1121, 1175, 1258, 1462
BciT130I CCWGG 1 cut(s) 1513
BciVI GTATCC 1 cut(s) 688
BclI TGATCA 2 cut(s) 48, 550
BcoDI GTCTC 6 cut(s) 142, 1003, 1048, 1138, 1302, 1413
BfaI CTAG 6 cut(s) 452, 677, 923, 1244, 1280, 1528
BfmI CTRYAG 2 cut(s) 363, 1090
BfuI GTATCC 1 cut(s) 688
BglII AGATCT 1 cut(s) 884
BisI GCNGC 1 cut(s) 660
BlsI GCNGC 1 cut(s) 661
Bme1390I CCNGG 1 cut(s) 1513
Bme18I GGWCC 1 cut(s) 1320
BmeT110I CYCGRG 1 cut(s) 689
BmgT120I GGNCC 1 cut(s) 1320
BmiI GGNNCC 1 cut(s) 1310
BmrFI CCNGG 1 cut(s) 1513
BmrI ACTGGG 1 cut(s) 1107
BmsI GCATC 2 cut(s) 461, 483
BmuI ACTGGG 1 cut(s) 1107
BpiI GAAGAC 3 cut(s) 233, 956, 1341
BpmI CTGGAG 1 cut(s) 1385
Bpu10I CCTNAGC 2 cut(s) 1062, 1404
BpuEI CTTGAG 1 cut(s) 395
BsaAI YACGTR 1 cut(s) 712
BsaBI GATNNNNATC 2 cut(s) 15, 1185
BsaI GGTCTC 2 cut(s) 142, 1003
BsaJI CCNNGG 5 cut(s) 429, 690, 1352, 1473, 1512
BsaWI WCCGGW 1 cut(s) 1311
BsaXI ACNNNNNCTCC 4 cut(s) 723, 753, 1412, 1442
Bsc4I CCNNNNNNNGG 4 cut(s) 1083, 1329, 1358, 1479
Bse1I ACTGG 3 cut(s) 1062, 1102, 1322
Bse8I GATNNNNATC 2 cut(s) 15, 1185
BseAI TCCGGA 1 cut(s) 1311
BseBI CCWGG 1 cut(s) 1513
BseDI CCNNGG 5 cut(s) 429, 690, 1352, 1473, 1512
BseGI GGATG 1 cut(s) 16
BseJI GATNNNNATC 2 cut(s) 15, 1185
BseLI CCNNNNNNNGG 4 cut(s) 1083, 1329, 1358, 1479
BseMII CTCAG 6 cut(s) 45, 72, 681, 1043, 1313, 1370
BseNI ACTGG 3 cut(s) 1062, 1102, 1322
BseRI GAGGAG 5 cut(s) 250, 470, 1033, 1036, 1464
BsgI GTGCAG 1 cut(s) 761
BshFI GGCC 2 cut(s) 1076, 1472
BsiHKCI CYCGRG 1 cut(s) 689
BsiSI CCGG 1 cut(s) 1312
BslI CCNNNNNNNGG 4 cut(s) 1083, 1329, 1358, 1479
BsmAI GTCTC 6 cut(s) 142, 1003, 1048, 1138, 1302, 1413
BsmBI CGTCTC 2 cut(s) 1302, 1413
BsmI GAATGC 1 cut(s) 608
BsnI GGCC 2 cut(s) 1076, 1472
Bso31I GGTCTC 2 cut(s) 142, 1003
BsoBI CYCGRG 1 cut(s) 689
Bsp13I TCCGGA 1 cut(s) 1311
Bsp1407I TGTACA 1 cut(s) 1345
Bsp143I GATC 6 cut(s) 48, 124, 511, 550, 884, 928
Bsp19I CCATGG 2 cut(s) 429, 1352
BspACI CCGC 2 cut(s) 659, 1015
BspANI GGCC 2 cut(s) 1076, 1472
BspCNI CTCAG 6 cut(s) 44, 71, 680, 1044, 1312, 1371
BspEI TCCGGA 1 cut(s) 1311
BspLI GGNNCC 1 cut(s) 1310
BspMAI CTGCAG 1 cut(s) 367
BspPI GGATC 2 cut(s) 519, 936
BspTNI GGTCTC 2 cut(s) 142, 1003
BsrGI TGTACA 1 cut(s) 1345
BsrI ACTGG 3 cut(s) 1062, 1102, 1322
BssECI CCNNGG 5 cut(s) 429, 690, 1352, 1473, 1512
BssMI GATC 6 cut(s) 48, 124, 511, 550, 884, 928
BssT1I CCWWGG 3 cut(s) 429, 1352, 1473
Bst2UI CCWGG 1 cut(s) 1513
Bst4CI ACNGT 2 cut(s) 203, 992
BstAUI TGTACA 1 cut(s) 1345
BstBAI YACGTR 1 cut(s) 712
BstC8I GCNNGC 2 cut(s) 363, 606
BstDEI CTNAG 9 cut(s) 31, 58, 667, 979, 1052, 1062, 1299, 1379, 1404
BstDSI CCRYGG 2 cut(s) 429, 1352
BstF5I GGATG 1 cut(s) 16
BstKTI GATC 6 cut(s) 51, 127, 514, 553, 887, 931
BstMAI GTCTC 6 cut(s) 142, 1003, 1048, 1138, 1302, 1413
BstMBI GATC 6 cut(s) 48, 124, 511, 550, 884, 928
BstMWI GCNNNNNNNGC 2 cut(s) 656, 668
BstNI CCWGG 1 cut(s) 1513
BstNSI RCATGY 1 cut(s) 1015
BstSCI CCNGG 1 cut(s) 1511
BstSFI CTRYAG 2 cut(s) 363, 1090
BstV2I GAAGAC 3 cut(s) 233, 956, 1341
BstX2I RGATCY 2 cut(s) 884, 928
BstYI RGATCY 2 cut(s) 884, 928
BsuI GTATCC 1 cut(s) 688
BsuRI GGCC 2 cut(s) 1076, 1472
BtgI CCRYGG 2 cut(s) 429, 1352
BtgZI GCGATG 1 cut(s) 576
BtsCI GGATG 1 cut(s) 16
Cac8I GCNNGC 2 cut(s) 363, 606
Cfr13I GGNCC 1 cut(s) 1320
Csp6I GTAC 1 cut(s) 1346
CviAII CATG 7 cut(s) 7, 419, 430, 878, 1012, 1343, 1353
CviQI GTAC 1 cut(s) 1346
DdeI CTNAG 9 cut(s) 31, 58, 667, 979, 1052, 1062, 1299, 1379, 1404
DpnI GATC 6 cut(s) 50, 126, 513, 552, 886, 930
DpnII GATC 6 cut(s) 48, 124, 511, 550, 884, 928
EaeI YGGCCR 1 cut(s) 1074
Eco130I CCWWGG 3 cut(s) 429, 1352, 1473
Eco31I GGTCTC 2 cut(s) 142, 1003
Eco47I GGWCC 1 cut(s) 1320
Eco57I CTGAAG 2 cut(s) 333, 854
Eco88I CYCGRG 1 cut(s) 689
EcoRI GAATTC 1 cut(s) 974
EcoRII CCWGG 1 cut(s) 1511
EcoT14I CCWWGG 3 cut(s) 429, 1352, 1473
EcoT22I ATGCAT 1 cut(s) 476
ErhI CCWWGG 3 cut(s) 429, 1352, 1473
Esp3I CGTCTC 2 cut(s) 1302, 1413
FaeI CATG 7 cut(s) 10, 422, 433, 881, 1015, 1346, 1356
FalI AAGNNNNNCTT 2 cut(s) 225, 257
FatI CATG 7 cut(s) 6, 418, 429, 877, 1011, 1342, 1352
FauNDI CATATG 1 cut(s) 1288
FbaI TGATCA 2 cut(s) 48, 550
FblI GTMKAC 1 cut(s) 1375
Fnu4HI GCNGC 1 cut(s) 660
FokI GGATG 1 cut(s) 23
Fsp4HI GCNGC 1 cut(s) 660
FspBI CTAG 6 cut(s) 452, 677, 923, 1244, 1280, 1528
GluI GCNGC 1 cut(s) 660
GsuI CTGGAG 1 cut(s) 1385
HaeIII GGCC 2 cut(s) 1076, 1472
HapII CCGG 1 cut(s) 1312
Hin1II CATG 7 cut(s) 10, 422, 433, 881, 1015, 1346, 1356
HindIII AAGCTT 1 cut(s) 762
HpaII CCGG 1 cut(s) 1312
HphI GGTGA 3 cut(s) 293, 316, 1273
Hpy166II GTNNAC 2 cut(s) 709, 1376
Hpy188III TCNNGA 3 cut(s) 536, 689, 1312
Hpy8I GTNNAC 2 cut(s) 709, 1376
HpyAV CCTTC 7 cut(s) 218, 227, 255, 272, 728, 898, 994
HpyCH4III ACNGT 2 cut(s) 203, 992
HpyCH4IV ACGT 1 cut(s) 711
HpyF10VI GCNNNNNNNGC 2 cut(s) 656, 668
HpyF3I CTNAG 9 cut(s) 31, 58, 667, 979, 1052, 1062, 1299, 1379, 1404
HpySE526I ACGT 1 cut(s) 711
Hsp92II CATG 7 cut(s) 10, 422, 433, 881, 1015, 1346, 1356
Kpn2I TCCGGA 1 cut(s) 1311
Ksp22I TGATCA 2 cut(s) 48, 550
Kzo9I GATC 6 cut(s) 48, 124, 511, 550, 884, 928
LmnI GCTCC 1 cut(s) 1433
LweI GCATC 2 cut(s) 461, 483
MaeI CTAG 6 cut(s) 452, 677, 923, 1244, 1280, 1528
MaeII ACGT 1 cut(s) 711
MaeIII GTNAC 4 cut(s) 304, 747, 793, 1114
MalI GATC 6 cut(s) 50, 126, 513, 552, 886, 930
MboI GATC 6 cut(s) 48, 124, 511, 550, 884, 928
MboII GAAGA 9 cut(s) 33, 185, 233, 398, 824, 830, 855, 961, 1346
MfeI CAATTG 1 cut(s) 495
MflI RGATCY 2 cut(s) 884, 928
MlsI TGGCCA 1 cut(s) 1076
MluNI TGGCCA 1 cut(s) 1076
MlyI GAGTC 5 cut(s) 247, 323, 689, 771, 1223
MmeI TCCRAC 4 cut(s) 105, 1140, 1377, 1396
Mox20I TGGCCA 1 cut(s) 1076
Mph1103I ATGCAT 1 cut(s) 476
MroI TCCGGA 1 cut(s) 1311
MscI TGGCCA 1 cut(s) 1076
MseI TTAA 3 cut(s) 804, 896, 1208
Msp20I TGGCCA 1 cut(s) 1076
MspI CCGG 1 cut(s) 1312
MspR9I CCNGG 1 cut(s) 1513
MunI CAATTG 1 cut(s) 495
Mva1269I GAATGC 1 cut(s) 608
MvaI CCWGG 1 cut(s) 1513
MwoI GCNNNNNNNGC 2 cut(s) 656, 668
NcoI CCATGG 2 cut(s) 429, 1352
NdeI CATATG 1 cut(s) 1288
NdeII GATC 6 cut(s) 48, 124, 511, 550, 884, 928
NlaIII CATG 7 cut(s) 10, 422, 433, 881, 1015, 1346, 1356
NlaIV GGNNCC 1 cut(s) 1310
NmuCI GTSAC 4 cut(s) 304, 747, 793, 1114
NsiI ATGCAT 1 cut(s) 476
NspI RCATGY 1 cut(s) 1015
PctI GAATGC 1 cut(s) 608
PfeI GAWTC 7 cut(s) 130, 389, 425, 874, 1186, 1223, 1489
PflFI GACNNNGTC 1 cut(s) 485
PflMI CCANNNNNTGG 1 cut(s) 1358
PkrI GCNGC 1 cut(s) 661
PleI GAGTC 5 cut(s) 246, 323, 688, 771, 1222
PpsI GAGTC 5 cut(s) 246, 323, 688, 771, 1222
Ppu21I YACGTR 1 cut(s) 712
Psp6I CCWGG 1 cut(s) 1511
PspGI CCWGG 1 cut(s) 1511
PspN4I GGNNCC 1 cut(s) 1310
PspPI GGNCC 1 cut(s) 1320
PstI CTGCAG 1 cut(s) 367
PsuI RGATCY 2 cut(s) 884, 928
PsyI GACNNNGTC 1 cut(s) 485
RsaI GTAC 1 cut(s) 1347
RsaNI GTAC 1 cut(s) 1346
SaqAI TTAA 3 cut(s) 804, 896, 1208
SatI GCNGC 1 cut(s) 660
Sau3AI GATC 6 cut(s) 48, 124, 511, 550, 884, 928
Sau96I GGNCC 1 cut(s) 1320
SchI GAGTC 5 cut(s) 247, 323, 689, 771, 1223
ScrFI CCNGG 1 cut(s) 1513
SfaNI GCATC 2 cut(s) 461, 483
SfcI CTRYAG 2 cut(s) 363, 1090
SinI GGWCC 1 cut(s) 1320
SmlI CTYRAG 1 cut(s) 410
SmoI CTYRAG 1 cut(s) 410
SsiI CCGC 2 cut(s) 659, 1015
SspMI CTAG 6 cut(s) 452, 677, 923, 1244, 1280, 1528
StyD4I CCNGG 1 cut(s) 1511
StyI CCWWGG 3 cut(s) 429, 1352, 1473
TaaI ACNGT 2 cut(s) 203, 992
TaiI ACGT 1 cut(s) 714
TaqI TCGA 2 cut(s) 332, 535
TaqII GACCGA 1 cut(s) 1337
TatI WGTACW 1 cut(s) 1345
TauI GCSGC 1 cut(s) 662
TfiI GAWTC 7 cut(s) 130, 389, 425, 874, 1186, 1223, 1489
Tru1I TTAA 3 cut(s) 804, 896, 1208
Tru9I TTAA 3 cut(s) 804, 896, 1208
TseFI GTSAC 4 cut(s) 304, 747, 793, 1114
Tsp45I GTSAC 4 cut(s) 304, 747, 793, 1114
TspDTI ATGAA 5 cut(s) 17, 78, 179, 288, 831
Tth111I GACNNNGTC 1 cut(s) 485
Van91I CCANNNNNTGG 1 cut(s) 1358
VpaK11BI GGWCC 1 cut(s) 1320
XapI RAATTY 8 cut(s) 65, 166, 176, 295, 595, 974, 1046, 1228
XceI RCATGY 1 cut(s) 1015
XmiI GTMKAC 1 cut(s) 1375
XspI CTAG 6 cut(s) 452, 677, 923, 1244, 1280, 1528
Zsp2I ATGCAT 1 cut(s) 476
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.