Rh3AG113800

Belongs to the GRAS family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3A
OG0004862
Physical Location & Seq
Reverse (-)
9641485 .. 9643522
2038 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3AG113800.1

Sequence Viewer

Length: 1608 bp
ATGGAATCTGCATTTCTTGAGCCTTGGTTTTTCGAAAATTTTATAGACTCATCTTTTGACCAACCTGATTTCATCAACATGGATGATACTCCAATTGATAGCCCAGACTTCTCTTCTCTGTTCAACTCAAATGAAGTTTTCAAAATTTCGGAAATTGCATCAGACCCTTTTGCCACCCTGTTTCCTTGTGATGATCTTATTCAAGTGACATCCCAAGTGGAGGAAGTTTCAATGGATTTGGAAGCATATGAGACTAATTTGATGATAGAGGGTTTCTATGGTAATTCATTGGAAGGGAGTGAAGGGAGTTTTCCATCACAGCAAGCTTCTTCACACCAATTTTCTGCTGAAGGAAATGATGTGTGGAGTCCAAATTCAATAGTGAATTCTGAAGCATCATCAATGGACTCAACATCAATCCAATCACCACTAAATTTGCCTAGAGAAGAGGAGGGAATCGAGAACCAATTGAGTATTTGTCATCTACTTCAAGCTTTTGCTGAAGCCAAGGAATATGGCCAGAAAGATCTAGAGGAGGTTATTCTCAGCTGCATCAGTGAGAAAGTCAGCCCAATTGGAGAGTCTTTCGAACGCCTTGCATTCAACTTGTGCCAAGAAGTTGTTGATGAACATCAACAAGGCGGCGATTATCTCAAACAAGAATCCTTGAAGAATTTTGAGGCTGCATTTAAGGTGTTCATCCAAAACTTCCCCTATGCTAGGTTTGCTCACTATGTAGCAAACTCAGCAATCCTCGAGGCGATACCAGCGGAAGCTGAGGCAATTCACATTGTGGATTTCGACATGAGAGAAGGGGTCCAGTTGTCTCAACTTATTGAGGCTTTAGCACAAAGATTCAAAACACTAAAAGTGACAGCAATCAAGTGGGAAGAGGAAGAGAGTGATTGTGCTCCTCCACACGGGAGATTTGAGGAGACAAAGAAGCAACTCCAGAACCATGCTAGATCCTTTGGCTTAAACTTAAAGGTGGAAGAAGTAGCAATGGAGGACTTGGTGACTGAGATAAAGAAGGCAAAGAAGAGGGGTGGAGGGAAAGAATTCTTAGCCTTCAACTGTATGGTTTCACTTCCACACATGAGGAGGAGAAGAAGCAGAGGGCATGTCATGGAGTATCTGAGACTGGCTAAGGAGTTAGCCAATTCTGGGAACTCCAAGACTACTAAAAGTGGTATTATAACTTTCGGTGATGGGGATGCTTGTGAGAGACTTGGAAATCACTCGAGTTTCAGCTCATTCTTTAATGAGAATCTAGTGCATTACCAAGCCTTGTTGGAGTCAATGGAGTTGAATTTCCCAAGTCATCTGGCAGAAGCAAGAATGGCGATGGAATGCATCTTCATAGCACCTTTTGTCTCTTCCCAGGCTTGGTTCCAAAAGTGGACAGAAATGAAGGACTGCCATGTTCAACCATGGTTCGGACTTGAGGGTCGGAGATTGAGCAAAGAAAATTTGATGGAAGCCAAGGAAATTGTGGGAGATGAAAGATCCTATGGAATCAGGATTGAAGGACTCAATGGGAATGAAATGGTATTGGAATGGAGAGGAACTCCTCTGGTTAGAGTATCAACTTGGGAAAACCAAAGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GRAS

Protein Analysis

535

Amino Acids

60.6

Weight (kDa)

4.69

Isoelectric Point (pI)

49.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRAS PF03514 161 - 532 1.8e-44 GRAS domain family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1196
AasI GACNNNNNNGTC 1 cut(s) 1446
AbsI CCTCGAGG 1 cut(s) 755
AciI CCGC 2 cut(s) 642, 770
AclWI GGATC 2 cut(s) 960, 1500
AcoI YGGCCR 1 cut(s) 517
AcsI RAATTY 9 cut(s) 37, 144, 373, 385, 433, 673, 1058, 1309, 1468
AcuI CTGAAG 3 cut(s) 369, 411, 522
AdeI CACNNNGTG 1 cut(s) 793
AfiI CCNNNNNNNGG 6 cut(s) 220, 720, 920, 1164, 1386, 1436
AjnI CCWGG 1 cut(s) 1380
AluBI AGCT 6 cut(s) 326, 494, 549, 776, 1251, 1605
AluI AGCT 6 cut(s) 326, 494, 549, 776, 1251, 1605
Alw21I GWGCWC 1 cut(s) 913
Alw26I GTCTC 6 cut(s) 245, 831, 929, 1132, 1219, 1378
AlwI GGATC 2 cut(s) 960, 1500
Ama87I CYCGRG 2 cut(s) 755, 1240
AoxI GGCC 1 cut(s) 517
ApeKI GCWGC 2 cut(s) 549, 683
ApoI RAATTY 9 cut(s) 37, 144, 373, 385, 433, 673, 1058, 1309, 1468
ArsI GACNNNNNNTTYG 2 cut(s) 38, 70
Asp700I GAANNNNTTC 1 cut(s) 1058
AspS9I GGNCC 1 cut(s) 817
AsuHPI GGTGA 3 cut(s) 417, 1027, 1217
AsuII TTCGAA 2 cut(s) 33, 588
AvaI CYCGRG 2 cut(s) 755, 1240
AvaII GGWCC 1 cut(s) 817
BalI TGGCCA 1 cut(s) 519
Bbv12I GWGCWC 1 cut(s) 913
BbvCI CCTCAGC 1 cut(s) 777
BbvI GCAGC 2 cut(s) 536, 670
BccI CCATC 4 cut(s) 322, 1202, 1339, 1468
BcgI CGANNNNNNTGC 2 cut(s) 578, 612
BciT130I CCWGG 1 cut(s) 1382
BcoDI GTCTC 6 cut(s) 245, 831, 929, 1132, 1219, 1378
BfaI CTAG 5 cut(s) 441, 530, 720, 963, 1271
BglII AGATCT 1 cut(s) 526
BisI GCNGC 3 cut(s) 550, 643, 684
BlsI GCNGC 3 cut(s) 551, 644, 685
Bme1390I CCNGG 1 cut(s) 1382
Bme18I GGWCC 1 cut(s) 817
BmeT110I CYCGRG 2 cut(s) 755, 1240
BmgT120I GGNCC 1 cut(s) 817
BmiI GGNNCC 2 cut(s) 818, 1391
BmrFI CCNGG 1 cut(s) 1382
BmsI GCATC 5 cut(s) 167, 404, 561, 1204, 1362
BplI GAGNNNNNCTC 2 cut(s) 1552, 1584
BpmI CTGGAG 1 cut(s) 935
Bpu10I CCTNAGC 2 cut(s) 777, 1146
Bpu14I TTCGAA 2 cut(s) 33, 588
BpuEI CTTGAG 2 cut(s) 38, 1463
BsaBI GATNNNNATC 1 cut(s) 630
BsaJI CCNNGG 5 cut(s) 23, 507, 1380, 1430, 1482
Bsc4I CCNNNNNNNGG 6 cut(s) 220, 720, 920, 1164, 1386, 1436
Bse1I ACTGG 2 cut(s) 820, 1146
Bse3DI GCAATG 1 cut(s) 1008
Bse8I GATNNNNATC 1 cut(s) 630
BseBI CCWGG 1 cut(s) 1382
BseDI CCNNGG 5 cut(s) 23, 507, 1380, 1430, 1482
BseGI GGATG 4 cut(s) 88, 209, 699, 1219
BseJI GATNNNNATC 1 cut(s) 630
BseLI CCNNNNNNNGG 6 cut(s) 220, 720, 920, 1164, 1386, 1436
BseMI GCAATG 1 cut(s) 1008
BseMII CTCAG 5 cut(s) 559, 759, 768, 1011, 1127
BseNI ACTGG 2 cut(s) 820, 1146
BseRI GAGGAG 7 cut(s) 464, 548, 903, 947, 1114, 1117, 1560
BseXI GCAGC 2 cut(s) 536, 670
BshFI GGCC 1 cut(s) 519
BsiHKAI GWGCWC 1 cut(s) 913
BsiHKCI CYCGRG 2 cut(s) 755, 1240
BslI CCNNNNNNNGG 6 cut(s) 220, 720, 920, 1164, 1386, 1436
BsmAI GTCTC 6 cut(s) 245, 831, 929, 1132, 1219, 1378
BsmI GAATGC 2 cut(s) 599, 1355
BsnI GGCC 1 cut(s) 519
BsoBI CYCGRG 2 cut(s) 755, 1240
Bsp119I TTCGAA 2 cut(s) 33, 588
Bsp1286I GDGCHC 1 cut(s) 913
Bsp143I GATC 4 cut(s) 193, 526, 965, 1505
Bsp19I CCATGG 1 cut(s) 1430
BspACI CCGC 2 cut(s) 642, 770
BspANI GGCC 1 cut(s) 519
BspCNI CTCAG 5 cut(s) 558, 758, 769, 1012, 1128
BspLI GGNNCC 2 cut(s) 818, 1391
BspPI GGATC 2 cut(s) 960, 1500
BspT104I TTCGAA 2 cut(s) 33, 588
BsrDI GCAATG 1 cut(s) 1008
BsrI ACTGG 2 cut(s) 820, 1146
BssECI CCNNGG 5 cut(s) 23, 507, 1380, 1430, 1482
BssMI GATC 4 cut(s) 193, 526, 965, 1505
BssT1I CCWWGG 4 cut(s) 23, 507, 1430, 1482
Bst2UI CCWGG 1 cut(s) 1382
Bst4CI ACNGT 1 cut(s) 1076
Bst6I CTCTTC 6 cut(s) 118, 441, 885, 891, 1034, 1381
BstBI TTCGAA 2 cut(s) 33, 588
BstC8I GCNNGC 1 cut(s) 324
BstDEI CTNAG 7 cut(s) 545, 745, 777, 1020, 1063, 1136, 1146
BstDSI CCRYGG 1 cut(s) 1430
BstENI CCTNNNNNAGG 1 cut(s) 718
BstF5I GGATG 4 cut(s) 88, 209, 699, 1219
BstKTI GATC 4 cut(s) 196, 529, 968, 1508
BstMAI GTCTC 6 cut(s) 245, 831, 929, 1132, 1219, 1378
BstMBI GATC 4 cut(s) 193, 526, 965, 1505
BstMWI GCNNNNNNNGC 4 cut(s) 725, 746, 767, 1340
BstNI CCWGG 1 cut(s) 1382
BstNSI RCATGY 1 cut(s) 1124
BstSCI CCNGG 1 cut(s) 1380
BstV1I GCAGC 2 cut(s) 536, 670
BstX2I RGATCY 3 cut(s) 526, 965, 1505
BstYI RGATCY 3 cut(s) 526, 965, 1505
BsuRI GGCC 1 cut(s) 519
BtgI CCRYGG 1 cut(s) 1430
BtgZI GCGATG 1 cut(s) 1358
BtsCI GGATG 4 cut(s) 88, 209, 699, 1219
BtsIMutI CAGTG 1 cut(s) 562
Cac8I GCNNGC 1 cut(s) 324
Cfr13I GGNCC 1 cut(s) 817
CspCI CAANNNNNGTGG 2 cut(s) 417, 452
CviAII CATG 8 cut(s) 79, 805, 959, 1096, 1121, 1126, 1421, 1431
DdeI CTNAG 7 cut(s) 545, 745, 777, 1020, 1063, 1136, 1146
DpnI GATC 4 cut(s) 195, 528, 967, 1507
DpnII GATC 4 cut(s) 193, 526, 965, 1505
DraIII CACNNNGTG 1 cut(s) 793
DrdI GACNNNNNNGTC 1 cut(s) 1446
DseDI GACNNNNNNGTC 1 cut(s) 1446
EaeI YGGCCR 1 cut(s) 517
Eam1104I CTCTTC 6 cut(s) 118, 441, 885, 891, 1034, 1381
EarI CTCTTC 6 cut(s) 118, 441, 885, 891, 1034, 1381
Eco130I CCWWGG 4 cut(s) 23, 507, 1430, 1482
Eco47I GGWCC 1 cut(s) 817
Eco57I CTGAAG 3 cut(s) 369, 411, 522
Eco88I CYCGRG 2 cut(s) 755, 1240
EcoNI CCTNNNNNAGG 1 cut(s) 718
EcoRI GAATTC 2 cut(s) 385, 1058
EcoRII CCWGG 1 cut(s) 1380
EcoT14I CCWWGG 4 cut(s) 23, 507, 1430, 1482
EcoT22I ATGCAT 1 cut(s) 1355
ErhI CCWWGG 4 cut(s) 23, 507, 1430, 1482
FaeI CATG 8 cut(s) 82, 808, 962, 1099, 1124, 1129, 1424, 1434
FatI CATG 8 cut(s) 78, 804, 958, 1095, 1120, 1125, 1420, 1430
FauNDI CATATG 1 cut(s) 247
Fnu4HI GCNGC 3 cut(s) 550, 643, 684
FokI GGATG 4 cut(s) 95, 196, 686, 1226
Fsp4HI GCNGC 3 cut(s) 550, 643, 684
FspBI CTAG 5 cut(s) 441, 530, 720, 963, 1271
GluI GCNGC 3 cut(s) 550, 643, 684
GsuI CTGGAG 1 cut(s) 935
HaeIII GGCC 1 cut(s) 519
Hin1II CATG 8 cut(s) 82, 808, 962, 1099, 1124, 1129, 1424, 1434
HindIII AAGCTT 2 cut(s) 324, 492
HphI GGTGA 3 cut(s) 417, 1027, 1217
Hpy166II GTNNAC 1 cut(s) 1401
Hpy188I TCNGA 6 cut(s) 151, 163, 391, 1137, 1439, 1452
Hpy188III TCNNGA 5 cut(s) 17, 460, 530, 952, 1519
Hpy8I GTNNAC 1 cut(s) 1401
HpyAV CCTTC 8 cut(s) 287, 296, 344, 806, 1024, 1078, 1405, 1520
HpyCH4III ACNGT 1 cut(s) 1076
HpyCH4V TGCA 7 cut(s) 11, 158, 552, 599, 686, 1276, 1353
HpyF10VI GCNNNNNNNGC 4 cut(s) 725, 746, 767, 1340
HpyF3I CTNAG 7 cut(s) 545, 745, 777, 1020, 1063, 1136, 1146
Hsp92II CATG 8 cut(s) 82, 808, 962, 1099, 1124, 1129, 1424, 1434
Kzo9I GATC 4 cut(s) 193, 526, 965, 1505
LmnI GCTCC 1 cut(s) 916
Lsp1109I GCAGC 2 cut(s) 536, 670
LweI GCATC 5 cut(s) 167, 404, 561, 1204, 1362
MaeI CTAG 5 cut(s) 441, 530, 720, 963, 1271
MaeIII GTNAC 3 cut(s) 205, 871, 1015
MalI GATC 4 cut(s) 195, 528, 967, 1507
MboI GATC 4 cut(s) 193, 526, 965, 1505
MfeI CAATTG 3 cut(s) 93, 467, 573
MflI RGATCY 3 cut(s) 526, 965, 1505
MhlI GDGCHC 1 cut(s) 913
MlsI TGGCCA 1 cut(s) 519
MluNI TGGCCA 1 cut(s) 519
MlyI GAGTC 6 cut(s) 41, 376, 401, 590, 1304, 1524
MmeI TCCRAC 2 cut(s) 1272, 1430
Mox20I TGGCCA 1 cut(s) 519
Mph1103I ATGCAT 1 cut(s) 1355
MroXI GAANNNNTTC 1 cut(s) 1058
MscI TGGCCA 1 cut(s) 519
MseI TTAA 4 cut(s) 690, 977, 983, 1260
MslI CAYNNNNRTG 1 cut(s) 77
Msp20I TGGCCA 1 cut(s) 519
MspA1I CMGCKG 2 cut(s) 549, 770
MspR9I CCNGG 1 cut(s) 1382
MunI CAATTG 3 cut(s) 93, 467, 573
Mva1269I GAATGC 2 cut(s) 599, 1355
MvaI CCWGG 1 cut(s) 1382
MwoI GCNNNNNNNGC 4 cut(s) 725, 746, 767, 1340
NcoI CCATGG 1 cut(s) 1430
NdeI CATATG 1 cut(s) 247
NdeII GATC 4 cut(s) 193, 526, 965, 1505
NlaIII CATG 8 cut(s) 82, 808, 962, 1099, 1124, 1129, 1424, 1434
NlaIV GGNNCC 2 cut(s) 818, 1391
NmuCI GTSAC 3 cut(s) 205, 871, 1015
NsiI ATGCAT 1 cut(s) 1355
NspI RCATGY 1 cut(s) 1124
NspV TTCGAA 2 cut(s) 33, 588
PaeR7I CTCGAG 2 cut(s) 755, 1240
PctI GAATGC 2 cut(s) 599, 1355
PdmI GAANNNNTTC 1 cut(s) 1058
PfeI GAWTC 6 cut(s) 5, 456, 662, 855, 1267, 1515
PkrI GCNGC 3 cut(s) 551, 644, 685
PleI GAGTC 6 cut(s) 41, 375, 401, 589, 1303, 1524
PpsI GAGTC 6 cut(s) 41, 375, 401, 589, 1303, 1524
PsiI TTATAA 1 cut(s) 1196
Psp6I CCWGG 1 cut(s) 1380
PspGI CCWGG 1 cut(s) 1380
PspN4I GGNNCC 2 cut(s) 818, 1391
PspPI GGNCC 1 cut(s) 817
PspXI VCTCGAGB 2 cut(s) 755, 1240
PsuI RGATCY 3 cut(s) 526, 965, 1505
PvuII CAGCTG 1 cut(s) 549
RseI CAYNNNNRTG 1 cut(s) 77
SaqAI TTAA 4 cut(s) 690, 977, 983, 1260
SatI GCNGC 3 cut(s) 550, 643, 684
Sau3AI GATC 4 cut(s) 193, 526, 965, 1505
Sau96I GGNCC 1 cut(s) 817
SchI GAGTC 6 cut(s) 41, 376, 401, 590, 1304, 1524
ScrFI CCNGG 1 cut(s) 1382
SduI GDGCHC 1 cut(s) 913
SfaNI GCATC 5 cut(s) 167, 404, 561, 1204, 1362
Sfr274I CTCGAG 2 cut(s) 755, 1240
SfuI TTCGAA 2 cut(s) 33, 588
SinI GGWCC 1 cut(s) 817
SlaI CTCGAG 2 cut(s) 755, 1240
SmiMI CAYNNNNRTG 1 cut(s) 77
SmlI CTYRAG 4 cut(s) 17, 755, 1240, 1442
SmoI CTYRAG 4 cut(s) 17, 755, 1240, 1442
SsiI CCGC 2 cut(s) 642, 770
SspMI CTAG 5 cut(s) 441, 530, 720, 963, 1271
StyD4I CCNGG 1 cut(s) 1380
StyI CCWWGG 4 cut(s) 23, 507, 1430, 1482
TaaI ACNGT 1 cut(s) 1076
TaqI TCGA 6 cut(s) 33, 459, 588, 756, 801, 1241
TauI GCSGC 1 cut(s) 645
TfiI GAWTC 6 cut(s) 5, 456, 662, 855, 1267, 1515
Tru1I TTAA 4 cut(s) 690, 977, 983, 1260
Tru9I TTAA 4 cut(s) 690, 977, 983, 1260
TscAI CASTG 1 cut(s) 562
TseFI GTSAC 3 cut(s) 205, 871, 1015
TseI GCWGC 2 cut(s) 549, 683
Tsp45I GTSAC 3 cut(s) 205, 871, 1015
TspDTI ATGAA 9 cut(s) 61, 147, 276, 642, 688, 1348, 1424, 1515, 1557
TspRI CASTG 1 cut(s) 562
VpaK11BI GGWCC 1 cut(s) 817
XagI CCTNNNNNAGG 1 cut(s) 718
XapI RAATTY 9 cut(s) 37, 144, 373, 385, 433, 673, 1058, 1309, 1468
XbaI TCTAGA 1 cut(s) 529
XceI RCATGY 1 cut(s) 1124
XcmI CCANNNNNNNNNTGG 1 cut(s) 1489
XhoI CTCGAG 2 cut(s) 755, 1240
XmnI GAANNNNTTC 1 cut(s) 1058
XspI CTAG 5 cut(s) 441, 530, 720, 963, 1271
Zsp2I ATGCAT 1 cut(s) 1355
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.