Rh3BG116700

Belongs to the GRAS family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3B
OG0004862
Physical Location & Seq
Reverse (-)
9604653 .. 9606260
1608 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3BG116700.1

Sequence Viewer

Length: 1608 bp
ATGGAATACTCTGCACTTCTTGAACCTTCTTGGCCATTCCACAATTTGGTGAACTCCCCTTTTGATCAATCTGATCCCATGAACATGGATGATACTCAAATGGGTAGCTCAGATTTTTCTTCTTTGATCATCAACTCAGATGAATTTTCTGACTTATCCGACACTTGTTCAGACCCTTTTGCCTCCTTGTTTTCTGGTAATGATCTGATTCAGGTCTCATCACAGGTGGAGGTATTCCCAATGAATTTGGAAGAATTTGACCCTAATTTGATGATAGACTGTTTGTATGGTAGTTTCTTGGAAGGGAGTGAAGGGAGTCTTCCTTCACAAGAAATCTCCTCACAATTTTCTATTGAAGGTGATTGTGTGTCAAATTCATTGGTGACTTCTGAAGTATCATCAATGGACTCGACATCAATCCAAACATCACTAACTTTGCCTGCAGAAGTGGTGGAAATAGATAACGAATCAAGTATTCGTCATCTTCTCAAGGCTCATGCAGAATCCATGGCAAATGGACAGAAAGAGCTAGAGGAGGTAATTTTCAGATGCATCAAGGACAAAGTCAGCCCAATTGGGCAGAGTTTGGATCGTCTTGCATTTCACTTGTGTCGAGAAGTTGTTGATGATCAACAAGGCGATGTTGATTATCTGAAACAACAATCCTACAAGAATTTTGAGCCTGCATTCAAGTTGTTCTACCAAAACTTTCCCTACGGGAAGTTTGCTCACTATGCGGCAAACTCAGCAATCCTAGAGTCTATTCCCGAGGATACAGAGATGGTTCACGTAGTAGATTTTGATATGGGAGAAGGGGTGCAGTTGTCACAGATGATTGAAGCTTTAGCACAAAGACTCAAAACATTGAGAGTGACAGCAATTAAATGGGAAGATGAAGAAAGTGATTGTGTTCTACAAGGAAGATTTGACGAGAGAAAGAAGCAACTTCAGAATCATGCAAGATCTTTTGGCTTAAACTTGAAGGTGGAGGAAGTTGCACTAGAGGATCTGGTGGGAGAGATAAAGAAGACAAAAAAGAGGGGTGTAAAAGAATTCTTAGCCTTCAACTGTATGGTCTCGCTTCCACACATGCGGAGGAGGAGAAGCCGAAGTCTTGCATTGGAATTTCTGAGACTGGCTAAGGATTTGTTGGCCAAATCAGGGAACTACAGGACTGGGAAAACAGGCATTGTGACCTTTGGAGATGGGGAGGCTTGTGGGAGACTTGAAAATCAGTTGGATTTCAGCTCTTTCTTTGATGGGAATCTGGTGCATTACCAAGCCTTAACAGAGTCAATGGAATCAAATTTTCCAAGTCATCTAGCAGAAGCAAGAATGGTGATGGAAAGCATTTTCCTAGCACCATATGTATCATCTCAGGCTTGGTTCCAGAACTGGACCGAAATGAGGGAAGACTGCCATGTACAACCATGGTTTGGGCTGGAGGGTCGTAGACTGAGCAAAGAGAACTTGTTGGAAGCTAAGGGCATTGTTGGAGACGAAAGCTCCTATGGAATTAGGATTGGAGGAGAGAATGGAAATGAGATGGCCTTGGAATGGAGAGGGAATCCTTTGGTCAGAGTTTCAACCTGGGAAAACCAAAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GRAS

Protein Analysis

535

Amino Acids

60.39

Weight (kDa)

4.65

Isoelectric Point (pI)

44.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRAS PF03514 160 - 532 4.6e-41 GRAS domain family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 46, 1436
AccI GTMKAC 1 cut(s) 1453
AciI CCGC 2 cut(s) 737, 1093
AclWI GGATC 3 cut(s) 68, 597, 1014
AcoI YGGCCR 2 cut(s) 32, 1152
AcsI RAATTY 8 cut(s) 143, 244, 254, 373, 673, 1052, 1124, 1306
AcuI CTGAAG 2 cut(s) 411, 932
AfaI GTAC 1 cut(s) 1425
AfiI CCNNNNNNNGG 5 cut(s) 46, 1161, 1407, 1436, 1557
AgsI TTSAA 8 cut(s) 23, 356, 691, 839, 982, 1066, 1229, 1587
AjnI CCWGG 1 cut(s) 1589
AluBI AGCT 7 cut(s) 108, 529, 842, 1248, 1481, 1506, 1605
AluI AGCT 7 cut(s) 108, 529, 842, 1248, 1481, 1506, 1605
Alw26I GTCTC 5 cut(s) 220, 1081, 1126, 1216, 1491
AlwI GGATC 3 cut(s) 68, 597, 1014
Ama87I CYCGRG 1 cut(s) 767
AoxI GGCC 3 cut(s) 32, 1152, 1548
ApoI RAATTY 8 cut(s) 143, 244, 254, 373, 673, 1052, 1124, 1306
AspS9I GGNCC 1 cut(s) 1398
AsuHPI GGTGA 4 cut(s) 61, 371, 394, 1351
AvaI CYCGRG 1 cut(s) 767
AvaII GGWCC 1 cut(s) 1398
BalI TGGCCA 2 cut(s) 34, 1154
BbsI GAAGAC 3 cut(s) 311, 1034, 1419
BccI CCATC 5 cut(s) 775, 1199, 1253, 1336, 1540
BciT130I CCWGG 1 cut(s) 1591
BciVI GTATCC 1 cut(s) 766
BclI TGATCA 3 cut(s) 64, 126, 628
BcoDI GTCTC 5 cut(s) 220, 1081, 1126, 1216, 1491
BfaI CTAG 6 cut(s) 530, 755, 1001, 1322, 1358, 1606
BfmI CTRYAG 2 cut(s) 441, 1168
BfuI GTATCC 1 cut(s) 766
BglII AGATCT 1 cut(s) 962
BisI GCNGC 1 cut(s) 738
BlsI GCNGC 1 cut(s) 739
Bme1390I CCNGG 1 cut(s) 1591
Bme18I GGWCC 1 cut(s) 1398
BmeT110I CYCGRG 1 cut(s) 767
BmgT120I GGNCC 1 cut(s) 1398
BmiI GGNNCC 1 cut(s) 1388
BmrFI CCNGG 1 cut(s) 1591
BmrI ACTGGG 1 cut(s) 1185
BmsI GCATC 2 cut(s) 539, 561
BmuI ACTGGG 1 cut(s) 1185
BpiI GAAGAC 3 cut(s) 311, 1034, 1419
BpmI CTGGAG 1 cut(s) 1463
Bpu10I CCTNAGC 2 cut(s) 1140, 1482
BpuEI CTTGAG 1 cut(s) 473
BsaAI YACGTR 1 cut(s) 790
BsaBI GATNNNNATC 1 cut(s) 1263
BsaI GGTCTC 2 cut(s) 220, 1081
BsaJI CCNNGG 5 cut(s) 507, 768, 1430, 1551, 1590
BsaXI ACNNNNNCTCC 4 cut(s) 801, 831, 1490, 1520
Bsc4I CCNNNNNNNGG 5 cut(s) 46, 1161, 1407, 1436, 1557
Bse1I ACTGG 3 cut(s) 1140, 1180, 1400
Bse8I GATNNNNATC 1 cut(s) 1263
BseBI CCWGG 1 cut(s) 1591
BseDI CCNNGG 5 cut(s) 507, 768, 1430, 1551, 1590
BseGI GGATG 1 cut(s) 94
BseJI GATNNNNATC 1 cut(s) 1263
BseLI CCNNNNNNNGG 5 cut(s) 46, 1161, 1407, 1436, 1557
BseMII CTCAG 6 cut(s) 123, 150, 759, 1121, 1391, 1448
BseNI ACTGG 3 cut(s) 1140, 1180, 1400
BseRI GAGGAG 5 cut(s) 328, 548, 1111, 1114, 1542
BsgI GTGCAG 1 cut(s) 839
BshFI GGCC 3 cut(s) 34, 1154, 1550
BsiHKCI CYCGRG 1 cut(s) 767
BslI CCNNNNNNNGG 5 cut(s) 46, 1161, 1407, 1436, 1557
BsmAI GTCTC 5 cut(s) 220, 1081, 1126, 1216, 1491
BsmBI CGTCTC 1 cut(s) 1491
BsmI GAATGC 1 cut(s) 686
BsnI GGCC 3 cut(s) 34, 1154, 1550
Bso31I GGTCTC 2 cut(s) 220, 1081
BsoBI CYCGRG 1 cut(s) 767
Bsp1407I TGTACA 1 cut(s) 1423
Bsp143I GATC 8 cut(s) 64, 73, 126, 202, 589, 628, 962, 1006
Bsp19I CCATGG 2 cut(s) 507, 1430
BspACI CCGC 2 cut(s) 737, 1093
BspANI GGCC 3 cut(s) 34, 1154, 1550
BspCNI CTCAG 6 cut(s) 122, 149, 758, 1122, 1390, 1449
BspLI GGNNCC 1 cut(s) 1388
BspMAI CTGCAG 1 cut(s) 445
BspPI GGATC 3 cut(s) 68, 597, 1014
BspTNI GGTCTC 2 cut(s) 220, 1081
BsrGI TGTACA 1 cut(s) 1423
BsrI ACTGG 3 cut(s) 1140, 1180, 1400
BssECI CCNNGG 5 cut(s) 507, 768, 1430, 1551, 1590
BssMI GATC 8 cut(s) 64, 73, 126, 202, 589, 628, 962, 1006
BssT1I CCWWGG 3 cut(s) 507, 1430, 1551
Bst2UI CCWGG 1 cut(s) 1591
Bst4CI ACNGT 2 cut(s) 281, 1070
BstAUI TGTACA 1 cut(s) 1423
BstBAI YACGTR 1 cut(s) 790
BstC8I GCNNGC 2 cut(s) 441, 684
BstDEI CTNAG 9 cut(s) 109, 136, 745, 1057, 1130, 1140, 1377, 1457, 1482
BstDSI CCRYGG 2 cut(s) 507, 1430
BstF5I GGATG 1 cut(s) 94
BstKTI GATC 8 cut(s) 67, 76, 129, 205, 592, 631, 965, 1009
BstMAI GTCTC 5 cut(s) 220, 1081, 1126, 1216, 1491
BstMBI GATC 8 cut(s) 64, 73, 126, 202, 589, 628, 962, 1006
BstMWI GCNNNNNNNGC 2 cut(s) 734, 746
BstNI CCWGG 1 cut(s) 1591
BstNSI RCATGY 1 cut(s) 1093
BstSCI CCNGG 1 cut(s) 1589
BstSFI CTRYAG 2 cut(s) 441, 1168
BstV2I GAAGAC 3 cut(s) 311, 1034, 1419
BstX2I RGATCY 2 cut(s) 962, 1006
BstXI CCANNNNNNTGG 1 cut(s) 85
BstYI RGATCY 2 cut(s) 962, 1006
BsuI GTATCC 1 cut(s) 766
BsuRI GGCC 3 cut(s) 34, 1154, 1550
BtgI CCRYGG 2 cut(s) 507, 1430
BtgZI GCGATG 1 cut(s) 654
BtsCI GGATG 1 cut(s) 94
Cac8I GCNNGC 2 cut(s) 441, 684
Cfr13I GGNCC 1 cut(s) 1398
Csp6I GTAC 1 cut(s) 1424
CviAII CATG 8 cut(s) 79, 85, 497, 508, 956, 1090, 1421, 1431
CviQI GTAC 1 cut(s) 1424
DdeI CTNAG 9 cut(s) 109, 136, 745, 1057, 1130, 1140, 1377, 1457, 1482
DpnI GATC 8 cut(s) 66, 75, 128, 204, 591, 630, 964, 1008
DpnII GATC 8 cut(s) 64, 73, 126, 202, 589, 628, 962, 1006
EaeI YGGCCR 2 cut(s) 32, 1152
Eco130I CCWWGG 3 cut(s) 507, 1430, 1551
Eco31I GGTCTC 2 cut(s) 220, 1081
Eco47I GGWCC 1 cut(s) 1398
Eco57I CTGAAG 2 cut(s) 411, 932
Eco88I CYCGRG 1 cut(s) 767
EcoRI GAATTC 1 cut(s) 1052
EcoRII CCWGG 1 cut(s) 1589
EcoT14I CCWWGG 3 cut(s) 507, 1430, 1551
EcoT22I ATGCAT 1 cut(s) 554
ErhI CCWWGG 3 cut(s) 507, 1430, 1551
Esp3I CGTCTC 1 cut(s) 1491
FaeI CATG 8 cut(s) 82, 88, 500, 511, 959, 1093, 1424, 1434
FalI AAGNNNNNCTT 2 cut(s) 303, 335
FatI CATG 8 cut(s) 78, 84, 496, 507, 955, 1089, 1420, 1430
FauNDI CATATG 1 cut(s) 1366
FbaI TGATCA 3 cut(s) 64, 126, 628
FblI GTMKAC 1 cut(s) 1453
Fnu4HI GCNGC 1 cut(s) 738
FokI GGATG 1 cut(s) 101
Fsp4HI GCNGC 1 cut(s) 738
FspBI CTAG 6 cut(s) 530, 755, 1001, 1322, 1358, 1606
GluI GCNGC 1 cut(s) 738
GsuI CTGGAG 1 cut(s) 1463
HaeIII GGCC 3 cut(s) 34, 1154, 1550
Hin1II CATG 8 cut(s) 82, 88, 500, 511, 959, 1093, 1424, 1434
HindIII AAGCTT 1 cut(s) 840
HphI GGTGA 4 cut(s) 61, 371, 394, 1351
Hpy166II GTNNAC 3 cut(s) 52, 787, 1454
Hpy188III TCNNGA 4 cut(s) 20, 614, 767, 1390
Hpy8I GTNNAC 3 cut(s) 52, 787, 1454
HpyAV CCTTC 8 cut(s) 36, 296, 305, 333, 350, 806, 976, 1072
HpyCH4III ACNGT 2 cut(s) 281, 1070
HpyCH4IV ACGT 1 cut(s) 789
HpyF10VI GCNNNNNNNGC 2 cut(s) 734, 746
HpyF3I CTNAG 9 cut(s) 109, 136, 745, 1057, 1130, 1140, 1377, 1457, 1482
HpySE526I ACGT 1 cut(s) 789
Hsp92II CATG 8 cut(s) 82, 88, 500, 511, 959, 1093, 1424, 1434
Ksp22I TGATCA 3 cut(s) 64, 126, 628
Kzo9I GATC 8 cut(s) 64, 73, 126, 202, 589, 628, 962, 1006
LmnI GCTCC 1 cut(s) 1511
LweI GCATC 2 cut(s) 539, 561
MaeI CTAG 6 cut(s) 530, 755, 1001, 1322, 1358, 1606
MaeII ACGT 1 cut(s) 789
MaeIII GTNAC 4 cut(s) 382, 825, 871, 1192
MalI GATC 8 cut(s) 66, 75, 128, 204, 591, 630, 964, 1008
MboI GATC 8 cut(s) 64, 73, 126, 202, 589, 628, 962, 1006
MboII GAAGA 9 cut(s) 111, 263, 311, 476, 902, 908, 933, 1039, 1424
MfeI CAATTG 1 cut(s) 573
MflI RGATCY 2 cut(s) 962, 1006
MlsI TGGCCA 2 cut(s) 34, 1154
MluNI TGGCCA 2 cut(s) 34, 1154
MlyI GAGTC 5 cut(s) 325, 401, 767, 849, 1301
MmeI TCCRAC 4 cut(s) 183, 1218, 1455, 1474
Mox20I TGGCCA 2 cut(s) 34, 1154
Mph1103I ATGCAT 1 cut(s) 554
MscI TGGCCA 2 cut(s) 34, 1154
MseI TTAA 3 cut(s) 882, 974, 1286
MslI CAYNNNNRTG 1 cut(s) 83
Msp20I TGGCCA 2 cut(s) 34, 1154
MspR9I CCNGG 1 cut(s) 1591
MunI CAATTG 1 cut(s) 573
Mva1269I GAATGC 1 cut(s) 686
MvaI CCWGG 1 cut(s) 1591
MwoI GCNNNNNNNGC 2 cut(s) 734, 746
NcoI CCATGG 2 cut(s) 507, 1430
NdeI CATATG 1 cut(s) 1366
NdeII GATC 8 cut(s) 64, 73, 126, 202, 589, 628, 962, 1006
NlaIII CATG 8 cut(s) 82, 88, 500, 511, 959, 1093, 1424, 1434
NlaIV GGNNCC 1 cut(s) 1388
NmuCI GTSAC 4 cut(s) 382, 825, 871, 1192
NsiI ATGCAT 1 cut(s) 554
NspI RCATGY 1 cut(s) 1093
PctI GAATGC 1 cut(s) 686
PfeI GAWTC 7 cut(s) 208, 467, 503, 952, 1264, 1301, 1567
PflFI GACNNNGTC 1 cut(s) 563
PflMI CCANNNNNTGG 2 cut(s) 46, 1436
PkrI GCNGC 1 cut(s) 739
PleI GAGTC 5 cut(s) 324, 401, 766, 849, 1300
PpsI GAGTC 5 cut(s) 324, 401, 766, 849, 1300
Ppu21I YACGTR 1 cut(s) 790
Psp6I CCWGG 1 cut(s) 1589
PspGI CCWGG 1 cut(s) 1589
PspN4I GGNNCC 1 cut(s) 1388
PspPI GGNCC 1 cut(s) 1398
PstI CTGCAG 1 cut(s) 445
PsuI RGATCY 2 cut(s) 962, 1006
PsyI GACNNNGTC 1 cut(s) 563
RsaI GTAC 1 cut(s) 1425
RsaNI GTAC 1 cut(s) 1424
RseI CAYNNNNRTG 1 cut(s) 83
SaqAI TTAA 3 cut(s) 882, 974, 1286
SatI GCNGC 1 cut(s) 738
Sau3AI GATC 8 cut(s) 64, 73, 126, 202, 589, 628, 962, 1006
Sau96I GGNCC 1 cut(s) 1398
SchI GAGTC 5 cut(s) 325, 401, 767, 849, 1301
ScrFI CCNGG 1 cut(s) 1591
SfaNI GCATC 2 cut(s) 539, 561
SfcI CTRYAG 2 cut(s) 441, 1168
SinI GGWCC 1 cut(s) 1398
SmiMI CAYNNNNRTG 1 cut(s) 83
SmlI CTYRAG 1 cut(s) 488
SmoI CTYRAG 1 cut(s) 488
SsiI CCGC 2 cut(s) 737, 1093
SspMI CTAG 6 cut(s) 530, 755, 1001, 1322, 1358, 1606
StyD4I CCNGG 1 cut(s) 1589
StyI CCWWGG 3 cut(s) 507, 1430, 1551
TaaI ACNGT 2 cut(s) 281, 1070
TaiI ACGT 1 cut(s) 792
TaqI TCGA 2 cut(s) 410, 613
TaqII GACCGA 1 cut(s) 1415
TatI WGTACW 1 cut(s) 1423
TauI GCSGC 1 cut(s) 740
TfiI GAWTC 7 cut(s) 208, 467, 503, 952, 1264, 1301, 1567
Tru1I TTAA 3 cut(s) 882, 974, 1286
Tru9I TTAA 3 cut(s) 882, 974, 1286
TseFI GTSAC 4 cut(s) 382, 825, 871, 1192
Tsp45I GTSAC 4 cut(s) 382, 825, 871, 1192
TspDTI ATGAA 5 cut(s) 95, 156, 257, 366, 909
Tth111I GACNNNGTC 1 cut(s) 563
Van91I CCANNNNNTGG 2 cut(s) 46, 1436
VpaK11BI GGWCC 1 cut(s) 1398
XapI RAATTY 8 cut(s) 143, 244, 254, 373, 673, 1052, 1124, 1306
XceI RCATGY 1 cut(s) 1093
XmiI GTMKAC 1 cut(s) 1453
XspI CTAG 6 cut(s) 530, 755, 1001, 1322, 1358, 1606
Zsp2I ATGCAT 1 cut(s) 554
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.