FvH4_6g10950

Belongs to the GRAS family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0004862
Physical Location & Seq
Reverse (-)
6551600 .. 6553611
2012 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g10950.t1

Sequence Viewer

Length: 1626 bp
ATGGAGTACTCCCCATTTCTTGAGCGTTCTTGGTCATTCCAAAGTTTGATAGACTCATCTTTTGATCAATCAGCTGAATTCATCAACATGGATCATACTCCAATCGATAGCTCAGATTTCTCTTCTCTGTTCATCAACTCAAATGAATTTTCCGAAATTTCGGAAATTACTTCATATCCTTTTGCAAACTTGTTTACTGCTGCTGATGATCTGATTCAAGTGACATCCCAAGGGGAGGAAGTTTCAATGGATTTGGAAGGATGTGAGGCTGATTTGATGATAGAGGGTTTCTTATGTAATTCTTTGGAAGTAAGTGAAGGGAGTTTTCCATCACAGAAAGCTTCTTCACACCAATTTTCTACAGAAGGAAATGATGTATGGAGTCCTGATTCATTAGTGACTTCTGAAGCATCATCAATGGACTCAACATCAATCCAATCACCACTGACTTTTCCTAGAGAAGAAGAGAGAATCGAGAACGAATTGAGTATTCGCCATCTACTTCAAGCTTTTGCTGAAGCCATAGAATATGGCCAGAAAGAGTTGGTGGAGGTAATTCTCAGATGCATCAGTGAGAAAGCCAGCCCAACTGGAGAGGCTTTTGAGCGTCTTGCATTCAACTTGTGCCAAGAAGTTGTTGATGAACAACAACAAGGTGGGGATTATCTCAAACAAGAATCCTTGAAGAATTTTGAGGCTGCACTTAAGGTATTCATCCAAAACTTCCCCTATGCTAGGTTTGCTCACTATGTAGCAAACTCAGCAATCCTCGACGCGATTCCAGTAGAAGCTGAGACGGTTCACATAGTGGATTTCGACATGGGAGATGGGATCCAGTTGTCTCAAATTATTGAGGCCTTAGCACAAAGATTCAAAACACTAAAAGTGACAGCAATTAAGTGGGAAGAGGAAGAGAGTGATTGTGCTCCTGCACACTGGAGATTTGAGGAGACCAAGAAGCAACTCCAGAACCATGCTAGATCCTTTGGCTTAAACTTAAAGCTGGAAGAAGTAGCAATGGAGGACTTGGTGACTGAGATAAAGAAGGCAAAGAAGAGGGGTGGAGGGAAAGAATTCTTAGCCTTCAACTGTATGAATTCACTTCCACATATGAGGAGGAGAAAAAGCAGAAGGCATGTCATGGAGTATCTGAGACTGGCTAAGGAGTTATTAGCCAGTTCTGGGAACTCCAAGACTACTAAGAGTGGTATCATAACTTTCGCTGATGGCAATGCTAGTGAGAATCTTGAAAATCACTCAAGTTTCAGCTCATTCTTTAATGAGAATCTAGTGCATTACCAAGCCTTGTTGGAGTCAATGGAGTTAAATTTCCCAAGTCATCTAGCTGAAGCAAGAATGGTCATGGAATGCATCTTCATAGCACCTTTTGTTTCTTCCCAAGATTGGTTCCAACAGTGGATAGAAATGAAGGACTTCCATGTGCAACCATGGTTTGGGCTTGAGGGTCGAAGATTGAGCAAAGAAAATTTGATGGAAGCCAAGGAAATTGTGGGAGATGAAGGATCGTATGGAATCAGGATTGAAGGACGGAACAGGAATGAGATGGTACTGGAATGGAGAGGTACTCCTCTGGTTAGAGTATCAACTTGGGAAAACCAAAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GRAS

Protein Analysis

542

Amino Acids

61.58

Weight (kDa)

4.72

Isoelectric Point (pI)

53.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRAS PF03514 165 - 538 4.4e-44 GRAS domain family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1454
AccII CGCG 1 cut(s) 776
AclWI GGATC 5 cut(s) 99, 826, 839, 975, 1531
AcoI YGGCCR 1 cut(s) 532
AcsI RAATTY 8 cut(s) 77, 146, 156, 688, 1073, 1096, 1327, 1486
AcuI CTGAAG 3 cut(s) 426, 537, 1368
AdeI CACNNNGTG 1 cut(s) 808
AfaI GTAC 3 cut(s) 8, 1569, 1585
AfiI CCNNNNNNNGG 5 cut(s) 235, 735, 1182, 1404, 1454
AflII CTTAAG 1 cut(s) 704
AgsI TTSAA 9 cut(s) 218, 246, 506, 619, 685, 874, 1087, 1250, 1544
AluBI AGCT 9 cut(s) 74, 111, 341, 509, 791, 1003, 1269, 1346, 1623
AluI AGCT 9 cut(s) 74, 111, 341, 509, 791, 1003, 1269, 1346, 1623
Alw21I GWGCWC 1 cut(s) 928
Alw26I GTCTC 4 cut(s) 788, 846, 944, 1147
AlwI GGATC 5 cut(s) 99, 826, 839, 975, 1531
AoxI GGCC 2 cut(s) 532, 855
ApeKI GCWGC 2 cut(s) 200, 698
ApoI RAATTY 8 cut(s) 77, 146, 156, 688, 1073, 1096, 1327, 1486
ArsI GACNNNNNNTTYG 2 cut(s) 44, 76
Asp700I GAANNNNTTC 2 cut(s) 1073, 1433
AsuHPI GGTGA 2 cut(s) 432, 1042
BalI TGGCCA 1 cut(s) 534
BamHI GGATCC 1 cut(s) 831
Bbv12I GWGCWC 1 cut(s) 928
BbvI GCAGC 2 cut(s) 187, 685
BccI CCATC 6 cut(s) 337, 504, 821, 1220, 1486, 1558
BclI TGATCA 1 cut(s) 64
BcoDI GTCTC 4 cut(s) 788, 846, 944, 1147
BfaI CTAG 7 cut(s) 456, 735, 978, 1236, 1289, 1343, 1624
BfmI CTRYAG 1 cut(s) 360
BfrI CTTAAG 1 cut(s) 704
BisI GCNGC 2 cut(s) 201, 699
BlsI GCNGC 2 cut(s) 202, 700
BmcAI AGTACT 1 cut(s) 8
BmiI GGNNCC 2 cut(s) 833, 1409
BmsI GCATC 4 cut(s) 419, 554, 576, 1380
BplI GAGNNNNNCTC 2 cut(s) 1570, 1602
BpmI CTGGAG 3 cut(s) 612, 950, 958
Bpu10I CCTNAGC 2 cut(s) 859, 1161
BpuEI CTTGAG 3 cut(s) 41, 1243, 1481
Bsa29I ATCGAT 1 cut(s) 105
BsaI GGTCTC 1 cut(s) 944
BsaJI CCNNGG 3 cut(s) 229, 1448, 1500
Bsc4I CCNNNNNNNGG 5 cut(s) 235, 735, 1182, 1404, 1454
Bse1I ACTGG 7 cut(s) 595, 782, 835, 941, 1161, 1176, 1575
Bse3DI GCAATG 2 cut(s) 1023, 1237
BseCI ATCGAT 1 cut(s) 105
BseDI CCNNGG 3 cut(s) 229, 1448, 1500
BseGI GGATG 3 cut(s) 224, 266, 714
BseLI CCNNNNNNNGG 5 cut(s) 235, 735, 1182, 1404, 1454
BseMI GCAATG 2 cut(s) 1023, 1237
BseMII CTCAG 6 cut(s) 126, 574, 774, 783, 1026, 1142
BseNI ACTGG 7 cut(s) 595, 782, 835, 941, 1161, 1176, 1575
BseRI GAGGAG 4 cut(s) 962, 1129, 1132, 1578
BseXI GCAGC 2 cut(s) 187, 685
BsgI GTGCAG 2 cut(s) 684, 915
Bsh1236I CGCG 1 cut(s) 776
BshFI GGCC 2 cut(s) 534, 857
BshVI ATCGAT 1 cut(s) 105
BsiHKAI GWGCWC 1 cut(s) 928
BslI CCNNNNNNNGG 5 cut(s) 235, 735, 1182, 1404, 1454
BsmAI GTCTC 4 cut(s) 788, 846, 944, 1147
BsmBI CGTCTC 1 cut(s) 788
BsmI GAATGC 2 cut(s) 614, 1373
BsnI GGCC 2 cut(s) 534, 857
Bso31I GGTCTC 1 cut(s) 944
Bsp1286I GDGCHC 1 cut(s) 928
Bsp143I GATC 6 cut(s) 64, 91, 208, 831, 980, 1523
Bsp19I CCATGG 1 cut(s) 1448
BspANI GGCC 2 cut(s) 534, 857
BspCNI CTCAG 6 cut(s) 125, 573, 773, 784, 1027, 1143
BspDI ATCGAT 1 cut(s) 105
BspFNI CGCG 1 cut(s) 776
BspLI GGNNCC 2 cut(s) 833, 1409
BspPI GGATC 5 cut(s) 99, 826, 839, 975, 1531
BspTI CTTAAG 1 cut(s) 704
BspTNI GGTCTC 1 cut(s) 944
BsrDI GCAATG 2 cut(s) 1023, 1237
BsrI ACTGG 7 cut(s) 595, 782, 835, 941, 1161, 1176, 1575
BssECI CCNNGG 3 cut(s) 229, 1448, 1500
BssMI GATC 6 cut(s) 64, 91, 208, 831, 980, 1523
BssT1I CCWWGG 3 cut(s) 229, 1448, 1500
Bst4CI ACNGT 3 cut(s) 799, 1091, 1416
Bst6I CTCTTC 5 cut(s) 127, 459, 900, 906, 1049
BstAFI CTTAAG 1 cut(s) 704
BstC8I GCNNGC 1 cut(s) 583
BstDSI CCRYGG 1 cut(s) 1448
BstENI CCTNNNNNAGG 1 cut(s) 733
BstF5I GGATG 3 cut(s) 224, 266, 714
BstFNI CGCG 1 cut(s) 776
BstKTI GATC 6 cut(s) 67, 94, 211, 834, 983, 1526
BstMAI GTCTC 4 cut(s) 788, 846, 944, 1147
BstMBI GATC 6 cut(s) 64, 91, 208, 831, 980, 1523
BstMWI GCNNNNNNNGC 2 cut(s) 740, 761
BstNSI RCATGY 1 cut(s) 1139
BstSFI CTRYAG 1 cut(s) 360
BstUI CGCG 1 cut(s) 776
BstV1I GCAGC 2 cut(s) 187, 685
BstX2I RGATCY 2 cut(s) 831, 980
BstYI RGATCY 2 cut(s) 831, 980
Bsu15I ATCGAT 1 cut(s) 105
BsuRI GGCC 2 cut(s) 534, 857
BsuTUI ATCGAT 1 cut(s) 105
BtgI CCRYGG 1 cut(s) 1448
BtsCI GGATG 3 cut(s) 224, 266, 714
BtsIMutI CAGTG 4 cut(s) 443, 577, 934, 1421
Cac8I GCNNGC 1 cut(s) 583
ClaI ATCGAT 1 cut(s) 105
CseI GACGC 2 cut(s) 596, 782
Csp6I GTAC 3 cut(s) 7, 1568, 1584
CviAII CATG 8 cut(s) 88, 820, 974, 1136, 1141, 1363, 1439, 1449
CviQI GTAC 3 cut(s) 7, 1568, 1584
DpnI GATC 6 cut(s) 66, 93, 210, 833, 982, 1525
DpnII GATC 6 cut(s) 64, 91, 208, 831, 980, 1523
DraIII CACNNNGTG 1 cut(s) 808
EaeI YGGCCR 1 cut(s) 532
Eam1104I CTCTTC 5 cut(s) 127, 459, 900, 906, 1049
EarI CTCTTC 5 cut(s) 127, 459, 900, 906, 1049
Eco130I CCWWGG 3 cut(s) 229, 1448, 1500
Eco147I AGGCCT 1 cut(s) 857
Eco31I GGTCTC 1 cut(s) 944
Eco57I CTGAAG 3 cut(s) 426, 537, 1368
EcoNI CCTNNNNNAGG 1 cut(s) 733
EcoRI GAATTC 3 cut(s) 77, 1073, 1096
EcoT14I CCWWGG 3 cut(s) 229, 1448, 1500
EcoT22I ATGCAT 2 cut(s) 569, 1373
ErhI CCWWGG 3 cut(s) 229, 1448, 1500
Esp3I CGTCTC 1 cut(s) 788
FaeI CATG 8 cut(s) 91, 823, 977, 1139, 1144, 1366, 1442, 1452
FatI CATG 8 cut(s) 87, 819, 973, 1135, 1140, 1362, 1438, 1448
FauNDI CATATG 1 cut(s) 1110
FbaI TGATCA 1 cut(s) 64
Fnu4HI GCNGC 2 cut(s) 201, 699
FokI GGATG 3 cut(s) 211, 273, 701
Fsp4HI GCNGC 2 cut(s) 201, 699
FspBI CTAG 7 cut(s) 456, 735, 978, 1236, 1289, 1343, 1624
GluI GCNGC 2 cut(s) 201, 699
GsuI CTGGAG 3 cut(s) 612, 950, 958
HaeIII GGCC 2 cut(s) 534, 857
HgaI GACGC 2 cut(s) 596, 782
Hin1II CATG 8 cut(s) 91, 823, 977, 1139, 1144, 1366, 1442, 1452
HindIII AAGCTT 2 cut(s) 339, 507
HphI GGTGA 2 cut(s) 432, 1042
Hpy166II GTNNAC 2 cut(s) 195, 802
Hpy188I TCNGA 7 cut(s) 115, 154, 163, 213, 406, 563, 1152
Hpy188III TCNNGA 6 cut(s) 20, 386, 475, 967, 1247, 1537
Hpy8I GTNNAC 2 cut(s) 195, 802
Hpy99I CGWCG 1 cut(s) 776
HpyAV CCTTC 9 cut(s) 251, 311, 359, 1039, 1093, 1125, 1423, 1514, 1538
HpyCH4III ACNGT 3 cut(s) 799, 1091, 1416
HpyCH4V TGCA 8 cut(s) 185, 567, 614, 701, 932, 1294, 1371, 1444
HpyF10VI GCNNNNNNNGC 2 cut(s) 740, 761
Hsp92II CATG 8 cut(s) 91, 823, 977, 1139, 1144, 1366, 1442, 1452
Ksp22I TGATCA 1 cut(s) 64
Kzo9I GATC 6 cut(s) 64, 91, 208, 831, 980, 1523
LmnI GCTCC 1 cut(s) 931
Lsp1109I GCAGC 2 cut(s) 187, 685
LweI GCATC 4 cut(s) 419, 554, 576, 1380
MaeI CTAG 7 cut(s) 456, 735, 978, 1236, 1289, 1343, 1624
MaeIII GTNAC 4 cut(s) 220, 397, 886, 1030
MalI GATC 6 cut(s) 66, 93, 210, 833, 982, 1525
MboI GATC 6 cut(s) 64, 91, 208, 831, 980, 1523
MflI RGATCY 2 cut(s) 831, 980
MhlI GDGCHC 1 cut(s) 928
MlsI TGGCCA 1 cut(s) 534
MluNI TGGCCA 1 cut(s) 534
MlyI GAGTC 4 cut(s) 47, 391, 416, 1322
MmeI TCCRAC 2 cut(s) 1290, 1435
Mox20I TGGCCA 1 cut(s) 534
Mph1103I ATGCAT 2 cut(s) 569, 1373
MroXI GAANNNNTTC 2 cut(s) 1073, 1433
MscI TGGCCA 1 cut(s) 534
MseI TTAA 6 cut(s) 705, 897, 992, 998, 1278, 1325
MslI CAYNNNNRTG 1 cut(s) 86
Msp20I TGGCCA 1 cut(s) 534
MspA1I CMGCKG 1 cut(s) 74
MspCI CTTAAG 1 cut(s) 704
Mva1269I GAATGC 2 cut(s) 614, 1373
MvnI CGCG 1 cut(s) 776
MwoI GCNNNNNNNGC 2 cut(s) 740, 761
NcoI CCATGG 1 cut(s) 1448
NdeI CATATG 1 cut(s) 1110
NdeII GATC 6 cut(s) 64, 91, 208, 831, 980, 1523
NlaIII CATG 8 cut(s) 91, 823, 977, 1139, 1144, 1366, 1442, 1452
NlaIV GGNNCC 2 cut(s) 833, 1409
NmuCI GTSAC 4 cut(s) 220, 397, 886, 1030
NsiI ATGCAT 2 cut(s) 569, 1373
NspI RCATGY 1 cut(s) 1139
PceI AGGCCT 1 cut(s) 857
PctI GAATGC 2 cut(s) 614, 1373
PdmI GAANNNNTTC 2 cut(s) 1073, 1433
PfeI GAWTC 9 cut(s) 214, 389, 471, 677, 778, 870, 1243, 1285, 1533
PflMI CCANNNNNTGG 1 cut(s) 1454
PkrI GCNGC 2 cut(s) 202, 700
PleI GAGTC 4 cut(s) 47, 390, 416, 1321
PpsI GAGTC 4 cut(s) 47, 390, 416, 1321
PspN4I GGNNCC 2 cut(s) 833, 1409
PsuI RGATCY 2 cut(s) 831, 980
PvuII CAGCTG 1 cut(s) 74
RsaI GTAC 3 cut(s) 8, 1569, 1585
RsaNI GTAC 3 cut(s) 7, 1568, 1584
RseI CAYNNNNRTG 1 cut(s) 86
SaqAI TTAA 6 cut(s) 705, 897, 992, 998, 1278, 1325
SatI GCNGC 2 cut(s) 201, 699
Sau3AI GATC 6 cut(s) 64, 91, 208, 831, 980, 1523
ScaI AGTACT 1 cut(s) 8
SchI GAGTC 4 cut(s) 47, 391, 416, 1322
SduI GDGCHC 1 cut(s) 928
SfaNI GCATC 4 cut(s) 419, 554, 576, 1380
SfcI CTRYAG 1 cut(s) 360
SmiMI CAYNNNNRTG 1 cut(s) 86
SmlI CTYRAG 4 cut(s) 20, 704, 1258, 1460
SmoI CTYRAG 4 cut(s) 20, 704, 1258, 1460
SseBI AGGCCT 1 cut(s) 857
SspMI CTAG 7 cut(s) 456, 735, 978, 1236, 1289, 1343, 1624
StuI AGGCCT 1 cut(s) 857
StyI CCWWGG 3 cut(s) 229, 1448, 1500
TaaI ACNGT 3 cut(s) 799, 1091, 1416
TaqI TCGA 5 cut(s) 105, 474, 771, 816, 1468
TatI WGTACW 1 cut(s) 6
TfiI GAWTC 9 cut(s) 214, 389, 471, 677, 778, 870, 1243, 1285, 1533
Tru1I TTAA 6 cut(s) 705, 897, 992, 998, 1278, 1325
Tru9I TTAA 6 cut(s) 705, 897, 992, 998, 1278, 1325
TscAI CASTG 4 cut(s) 450, 577, 941, 1421
TseFI GTSAC 4 cut(s) 220, 397, 886, 1030
TseI GCWGC 2 cut(s) 200, 698
Tsp45I GTSAC 4 cut(s) 220, 397, 886, 1030
TspGWI ACGGA 1 cut(s) 1564
TspRI CASTG 4 cut(s) 450, 577, 941, 1421
Van91I CCANNNNNTGG 1 cut(s) 1454
Vha464I CTTAAG 1 cut(s) 704
XagI CCTNNNNNAGG 1 cut(s) 733
XapI RAATTY 8 cut(s) 77, 146, 156, 688, 1073, 1096, 1327, 1486
XceI RCATGY 1 cut(s) 1139
XcmI CCANNNNNNNNNTGG 1 cut(s) 1507
XmnI GAANNNNTTC 2 cut(s) 1073, 1433
XspI CTAG 7 cut(s) 456, 735, 978, 1236, 1289, 1343, 1624
ZrmI AGTACT 1 cut(s) 8
Zsp2I ATGCAT 2 cut(s) 569, 1373
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.