MD04G1163300.v1.1

Belongs to the GRAS family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0004862
Physical Location & Seq
Forward (+)
25473582 .. 25475192
1611 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1163300.v1.1.491

Sequence Viewer

Length: 1611 bp
ATGATGCAGTCTGAGATGAACTTTCAATCCTTGTGGCCCTACCTGAATGTCACAAATTCACCTATGGATACAAACGAATACAATGATCAGATGGCCAGCACTGAATTTTCTTCGATTTTCATGGCGTCTGACGAATTTTCAGAATCTTCTCCTTTTCCATTTTCAACCATTTTTTCTGGTGAATGTGAACAATTTGCGGACTGTGATAATCTCCTGCAAGCCACATCGATGGCGGCAGAGGAATTTCCGATGGAGTTGGGAGGATTCGAGCCGAATTTTTTTATCGATCAGTATGAAAATATGTATGCCTCATGTTTAGAAGGCAGTGAGGGAAGCAATACTCTTCCTTCACAGCAATTTTCTGTTGAAGGAAATGATGTGTGGAGTCCAAACTCCTTTGTGACATCAGAGGCATCCTCCATTGATGTGACCTCGGTTCAACAATCACTAACCCTACCAAGAGACGACATGGAAATTGACAACGAAGTGAGTATTCACCATCTGCTCAAGGCGTTCAGCGAGTCCATGGAGATGGGGCAGAGGGAGCTGGAGTTGGTTATAATGAGATGCCTTGCAGAGAAAGTAAACCCACTTGGTCAATCTCTTGAGCGCCTTGCATTCAACTTATGTCAAGGATTTGTTGATAATCAGCACGGAAACTATTTGAAACAAGAATCCTTCAATAATTTCGAGGCCGCATTCAACTTGTTCTACCAAATCTTCCCTTTTGGGAGGCTTGCTCACTACGCAGCAAACACTGCGATCCTCGAGTCTATCCCAGAAGATGTAGAGACGGTTCATGTAGTGGAATTTGATATTGGTGAAGGGGTTCAATTGTCACATCTGATTGAGGCTGTGGCAAAGAGAAACAAAACATTAAAAGTGACAGCAATTAAATGGGATGTGGAAGAGAGTGATGAGGTTGCTCCTCCACAGTGGAGATTCGAGGAGACGAAAAAGCAACTCCAGCATCATGCAAGGTCTTTCGGCCTAAACTTGAAGGTGGAGGAGATCGCGATCGAGGATTTGGTGAGCGAAATCAAGAAGGCGAACAAGAGAGGTGGAATGAGAGAATTTATAGCCTTCAACTCTATGGTAGGGCTTCCTCACATGAGGAGGAGAAGAAGCAGAGGACTTGTAATGGAATTCCTAAGGCTAGCTAAGGATTTATTAGCCAATTCAGCAAGAGGTATCGTAACCTTTGGTGATGGATATGCTTGCACGAAAAGGGGAAATGACTTGAGTTTCAGGTCCTTCTTTGACGTGAATGTTGTGCATTACAAAGCATTGTTAGAGTCTTTGGAATCGACCTTTCCGAGCCACCTAGGAGAGGCAAGGATGGTGATGGAGTTGATGTTTGTGGCGCCGTATGTGTCTTCTCAGGCTTGGCTCGATAAGTGGAACGAAGAACGAGAATGTGGAAATCTTCAGCCCTTGTTCGGGCTGGAGAGTAGGAGAGTGAGTAGGGAAATGTTAATGGAGGCCAAAGAAATTGTGGGGGCAGATAGCTCGTATGGAGTGAGAGTTGGAGGAGAAAATGGGAATGAGATGGCTTTGGAATGGAATTCAATTCCTTTGGTCAGAGTTGAAACCTGGACAAACGGAAGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GRAS

Protein Analysis

537

Amino Acids

60.73

Weight (kDa)

4.67

Isoelectric Point (pI)

53.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRAS PF03514 166 - 533 3.4e-36 GRAS domain family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 560
AccB1I GGYRCC 1 cut(s) 1363
AccII CGCG 1 cut(s) 1016
AciI CCGC 3 cut(s) 197, 233, 696
AclWI GGATC 1 cut(s) 757
AcoI YGGCCR 1 cut(s) 93
AcsI RAATTY 9 cut(s) 55, 104, 134, 242, 274, 809, 1073, 1145, 1564
AcuI CTGAAG 1 cut(s) 1412
AcyI GRCGYC 2 cut(s) 125, 1364
AfiI CCNNNNNNNGG 3 cut(s) 1330, 1439, 1440
AjiI CACGTC 1 cut(s) 1264
AjnI CCWGG 1 cut(s) 1592
AjuI GAANNNNNNNTTGG 2 cut(s) 801, 833
AloI GAACNNNNNNTCC 2 cut(s) 11, 43
AluBI AGCT 4 cut(s) 547, 1160, 1509, 1608
AluI AGCT 4 cut(s) 547, 1160, 1509, 1608
Alw26I GTCTC 3 cut(s) 456, 785, 944
AlwI GGATC 1 cut(s) 757
Ama87I CYCGRG 1 cut(s) 767
AoxI GGCC 5 cut(s) 35, 93, 693, 988, 1482
ApeKI GCWGC 1 cut(s) 749
ApoI RAATTY 9 cut(s) 55, 104, 134, 242, 274, 809, 1073, 1145, 1564
Asp700I GAANNNNTTC 1 cut(s) 828
AspA2I CCTAGG 1 cut(s) 1324
AspLEI GCGC 2 cut(s) 612, 1366
AspS9I GGNCC 2 cut(s) 36, 1251
AsuHPI GGTGA 7 cut(s) 51, 191, 488, 833, 1042, 1217, 1354
AsuNHI GCTAGC 1 cut(s) 1156
AvaI CYCGRG 1 cut(s) 767
AvaII GGWCC 1 cut(s) 1251
AvrII CCTAGG 1 cut(s) 1324
AxyI CCTNAGG 1 cut(s) 1151
BalI TGGCCA 1 cut(s) 95
BanI GGYRCC 1 cut(s) 1363
BbsI GAAGAC 1 cut(s) 1368
BbvI GCAGC 1 cut(s) 761
BccI CCATC 9 cut(s) 85, 223, 244, 507, 526, 1202, 1333, 1339, 1543
BceAI ACGGC 1 cut(s) 1351
BcgI CGANNNNNNTGC 2 cut(s) 1491, 1525
BciT130I CCWGG 1 cut(s) 1594
BciVI GTATCC 1 cut(s) 61
BclI TGATCA 1 cut(s) 85
BcoDI GTCTC 3 cut(s) 456, 785, 944
BfaI CTAG 2 cut(s) 1157, 1325
BfoI RGCGCY 2 cut(s) 613, 1367
BfuI GTATCC 1 cut(s) 61
BisI GCNGC 3 cut(s) 234, 696, 750
BlnI CCTAGG 1 cut(s) 1324
BlsI GCNGC 3 cut(s) 235, 697, 751
Bme1390I CCNGG 1 cut(s) 1594
Bme18I GGWCC 1 cut(s) 1251
BmeT110I CYCGRG 1 cut(s) 767
BmgBI CACGTC 1 cut(s) 1264
BmgT120I GGNCC 2 cut(s) 36, 1251
BmiI GGNNCC 1 cut(s) 1365
BmrFI CCNGG 1 cut(s) 1594
BmsI GCATC 3 cut(s) 422, 557, 979
BmtI GCTAGC 1 cut(s) 1160
BpiI GAAGAC 1 cut(s) 1368
BplI GAGNNNNNCTC 4 cut(s) 401, 433, 724, 756
BpmI CTGGAG 3 cut(s) 569, 950, 1466
Bpu10I CCTNAGC 1 cut(s) 1161
BpuEI CTTGAG 3 cut(s) 491, 626, 1261
Bsa29I ATCGAT 2 cut(s) 227, 285
BsaBI GATNNNNATC 1 cut(s) 1016
BsaHI GRCGYC 2 cut(s) 125, 1364
BsaJI CCNNGG 3 cut(s) 432, 525, 1324
BsaXI ACNNNNNCTCC 8 cut(s) 536, 566, 1340, 1370, 1447, 1477, 1509, 1539
Bsc4I CCNNNNNNNGG 3 cut(s) 1330, 1439, 1440
Bse21I CCTNAGG 1 cut(s) 1151
Bse8I GATNNNNATC 1 cut(s) 1016
BseBI CCWGG 1 cut(s) 1594
BseCI ATCGAT 2 cut(s) 227, 285
BseDI CCNNGG 3 cut(s) 432, 525, 1324
BseGI GGATG 3 cut(s) 413, 907, 1344
BseJI GATNNNNATC 1 cut(s) 1016
BseLI CCNNNNNNNGG 3 cut(s) 1330, 1439, 1440
BseMII CTCAG 1 cut(s) 1394
BseRI GAGGAG 6 cut(s) 918, 962, 1022, 1129, 1132, 1545
BseXI GCAGC 1 cut(s) 761
Bsh1236I CGCG 1 cut(s) 1016
Bsh1285I CGRYCG 1 cut(s) 1020
BshFI GGCC 5 cut(s) 37, 95, 695, 990, 1484
BshNI GGYRCC 1 cut(s) 1363
BshVI ATCGAT 2 cut(s) 227, 285
BsiEI CGRYCG 1 cut(s) 1020
BsiHKCI CYCGRG 1 cut(s) 767
BslI CCNNNNNNNGG 3 cut(s) 1330, 1439, 1440
BsmAI GTCTC 3 cut(s) 456, 785, 944
BsmBI CGTCTC 3 cut(s) 456, 785, 944
BsmI GAATGC 2 cut(s) 617, 698
BsnI GGCC 5 cut(s) 37, 95, 695, 990, 1484
BsoBI CYCGRG 1 cut(s) 767
Bsp143I GATC 5 cut(s) 85, 286, 762, 1011, 1017
Bsp19I CCATGG 1 cut(s) 525
Bsp68I TCGCGA 1 cut(s) 1016
BspACI CCGC 3 cut(s) 197, 233, 696
BspANI GGCC 5 cut(s) 37, 95, 695, 990, 1484
BspCNI CTCAG 2 cut(s) 4, 1393
BspDI ATCGAT 2 cut(s) 227, 285
BspFNI CGCG 1 cut(s) 1016
BspLI GGNNCC 1 cut(s) 1365
BspOI GCTAGC 1 cut(s) 1160
BspPI GGATC 1 cut(s) 757
BspT107I GGYRCC 1 cut(s) 1363
BssECI CCNNGG 3 cut(s) 432, 525, 1324
BssMI GATC 5 cut(s) 85, 286, 762, 1011, 1017
BssNI GRCGYC 2 cut(s) 125, 1364
BssT1I CCWWGG 2 cut(s) 525, 1324
Bst2UI CCWGG 1 cut(s) 1594
Bst4CI ACNGT 3 cut(s) 203, 796, 936
Bst6I CTCTTC 2 cut(s) 348, 903
BstACI GRCGYC 2 cut(s) 125, 1364
BstAPI GCANNNNNTGC 1 cut(s) 758
BstC8I GCNNGC 5 cut(s) 97, 219, 738, 1158, 1219
BstDEI CTNAG 4 cut(s) 12, 1151, 1161, 1380
BstDSI CCRYGG 1 cut(s) 525
BstENI CCTNNNNNAGG 1 cut(s) 1328
BstF5I GGATG 3 cut(s) 413, 907, 1344
BstFNI CGCG 1 cut(s) 1016
BstH2I RGCGCY 2 cut(s) 613, 1367
BstHHI GCGC 2 cut(s) 612, 1366
BstKTI GATC 5 cut(s) 88, 289, 765, 1014, 1020
BstMAI GTCTC 3 cut(s) 456, 785, 944
BstMBI GATC 5 cut(s) 85, 286, 762, 1011, 1017
BstMCI CGRYCG 1 cut(s) 1020
BstMWI GCNNNNNNNGC 5 cut(s) 544, 746, 758, 967, 1181
BstNI CCWGG 1 cut(s) 1594
BstSCI CCNGG 1 cut(s) 1592
BstUI CGCG 1 cut(s) 1016
BstV1I GCAGC 1 cut(s) 761
BstV2I GAAGAC 1 cut(s) 1368
BstXI CCANNNNNNTGG 2 cut(s) 229, 532
Bsu15I ATCGAT 2 cut(s) 227, 285
Bsu36I CCTNAGG 1 cut(s) 1151
BsuI GTATCC 1 cut(s) 61
BsuRI GGCC 5 cut(s) 37, 95, 695, 990, 1484
BsuTUI ATCGAT 2 cut(s) 227, 285
BtgI CCRYGG 1 cut(s) 525
BtrI CACGTC 1 cut(s) 1264
BtsCI GGATG 3 cut(s) 413, 907, 1344
BtsI GCAGTG 2 cut(s) 331, 756
BtsIMutI CAGTG 4 cut(s) 99, 331, 756, 941
BtuMI TCGCGA 1 cut(s) 1016
Cac8I GCNNGC 5 cut(s) 97, 219, 738, 1158, 1219
CfoI GCGC 2 cut(s) 612, 1366
Cfr13I GGNCC 2 cut(s) 36, 1251
ClaI ATCGAT 2 cut(s) 227, 285
CseI GACGC 1 cut(s) 114
CspCI CAANNNNNGTGG 4 cut(s) 14, 49, 1042, 1077
CviAII CATG 7 cut(s) 121, 312, 469, 526, 800, 974, 1111
DdeI CTNAG 4 cut(s) 12, 1151, 1161, 1380
DinI GGCGCC 1 cut(s) 1365
DpnI GATC 5 cut(s) 87, 288, 764, 1013, 1019
DpnII GATC 5 cut(s) 85, 286, 762, 1011, 1017
EaeI YGGCCR 1 cut(s) 93
Eam1104I CTCTTC 2 cut(s) 348, 903
EarI CTCTTC 2 cut(s) 348, 903
Eco130I CCWWGG 2 cut(s) 525, 1324
Eco47I GGWCC 1 cut(s) 1251
Eco57I CTGAAG 1 cut(s) 1412
Eco81I CCTNAGG 1 cut(s) 1151
Eco88I CYCGRG 1 cut(s) 767
EcoNI CCTNNNNNAGG 1 cut(s) 1328
EcoO109I RGGNCCY 1 cut(s) 1251
EcoRI GAATTC 2 cut(s) 1145, 1564
EcoRII CCWGG 1 cut(s) 1592
EcoT14I CCWWGG 2 cut(s) 525, 1324
EgeI GGCGCC 1 cut(s) 1365
EheI GGCGCC 1 cut(s) 1365
ErhI CCWWGG 2 cut(s) 525, 1324
Esp3I CGTCTC 3 cut(s) 456, 785, 944
FaeI CATG 7 cut(s) 124, 315, 472, 529, 803, 977, 1114
FatI CATG 7 cut(s) 120, 311, 468, 525, 799, 973, 1110
FbaI TGATCA 1 cut(s) 85
Fnu4HI GCNGC 3 cut(s) 234, 696, 750
FokI GGATG 3 cut(s) 400, 914, 1351
Fsp4HI GCNGC 3 cut(s) 234, 696, 750
FspBI CTAG 2 cut(s) 1157, 1325
GlaI GCGC 2 cut(s) 611, 1365
GluI GCNGC 3 cut(s) 234, 696, 750
GsuI CTGGAG 3 cut(s) 569, 950, 1466
HaeII RGCGCY 2 cut(s) 613, 1367
HaeIII GGCC 5 cut(s) 37, 95, 695, 990, 1484
HgaI GACGC 1 cut(s) 114
HhaI GCGC 2 cut(s) 612, 1366
Hin1I GRCGYC 2 cut(s) 125, 1364
Hin1II CATG 7 cut(s) 124, 315, 472, 529, 803, 977, 1114
Hin6I GCGC 2 cut(s) 610, 1364
HinP1I GCGC 2 cut(s) 610, 1364
HinfI GANTC 9 cut(s) 143, 264, 385, 521, 674, 770, 942, 1295, 1304
HphI GGTGA 7 cut(s) 51, 191, 488, 833, 1042, 1217, 1354
Hpy166II GTNNAC 2 cut(s) 188, 586
Hpy188I TCNGA 9 cut(s) 13, 90, 130, 142, 249, 409, 846, 1317, 1583
Hpy188III TCNNGA 3 cut(s) 605, 1015, 1042
Hpy8I GTNNAC 2 cut(s) 188, 586
HpyAV CCTTC 9 cut(s) 314, 357, 362, 688, 818, 994, 1039, 1093, 1264
HpyCH4III ACNGT 3 cut(s) 203, 796, 936
HpyCH4IV ACGT 1 cut(s) 1263
HpyCH4V TGCA 7 cut(s) 7, 217, 575, 617, 977, 1221, 1276
HpyF10VI GCNNNNNNNGC 5 cut(s) 544, 746, 758, 967, 1181
HpyF3I CTNAG 4 cut(s) 12, 1151, 1161, 1380
HpySE526I ACGT 1 cut(s) 1263
Hsp92I GRCGYC 2 cut(s) 125, 1364
Hsp92II CATG 7 cut(s) 124, 315, 472, 529, 803, 977, 1114
HspAI GCGC 2 cut(s) 610, 1364
KasI GGCGCC 1 cut(s) 1363
Ksp22I TGATCA 1 cut(s) 85
Kzo9I GATC 5 cut(s) 85, 286, 762, 1011, 1017
LmnI GCTCC 2 cut(s) 544, 931
Lsp1109I GCAGC 1 cut(s) 761
LweI GCATC 3 cut(s) 422, 557, 979
MaeI CTAG 2 cut(s) 1157, 1325
MaeII ACGT 1 cut(s) 1263
MaeIII GTNAC 6 cut(s) 49, 400, 427, 837, 883, 1195
MalI GATC 5 cut(s) 87, 288, 764, 1013, 1019
MboI GATC 5 cut(s) 85, 286, 762, 1011, 1017
MfeI CAATTG 1 cut(s) 833
MlsI TGGCCA 1 cut(s) 95
MluNI TGGCCA 1 cut(s) 95
Mly113I GGCGCC 1 cut(s) 1364
MlyI GAGTC 4 cut(s) 394, 530, 779, 1304
MmeI TCCRAC 1 cut(s) 1507
Mox20I TGGCCA 1 cut(s) 95
MroXI GAANNNNTTC 1 cut(s) 828
MscI TGGCCA 1 cut(s) 95
MseI TTAA 3 cut(s) 878, 894, 1475
MslI CAYNNNNRTG 3 cut(s) 227, 425, 530
Msp20I TGGCCA 1 cut(s) 95
MspR9I CCNGG 1 cut(s) 1594
MunI CAATTG 1 cut(s) 833
Mva1269I GAATGC 2 cut(s) 617, 698
MvaI CCWGG 1 cut(s) 1594
MvnI CGCG 1 cut(s) 1016
MwoI GCNNNNNNNGC 5 cut(s) 544, 746, 758, 967, 1181
NarI GGCGCC 1 cut(s) 1364
NcoI CCATGG 1 cut(s) 525
NdeII GATC 5 cut(s) 85, 286, 762, 1011, 1017
NheI GCTAGC 1 cut(s) 1156
NlaIII CATG 7 cut(s) 124, 315, 472, 529, 803, 977, 1114
NlaIV GGNNCC 1 cut(s) 1365
NmuCI GTSAC 5 cut(s) 49, 400, 427, 837, 883
NruI TCGCGA 1 cut(s) 1016
PaeR7I CTCGAG 1 cut(s) 767
PcsI WCGNNNNNNNCGW 1 cut(s) 1313
PctI GAATGC 2 cut(s) 617, 698
PdmI GAANNNNTTC 1 cut(s) 828
PfeI GAWTC 5 cut(s) 143, 264, 674, 942, 1304
PkrI GCNGC 3 cut(s) 235, 697, 751
Ple19I CGATCG 1 cut(s) 1020
PleI GAGTC 4 cut(s) 393, 529, 778, 1303
PluTI GGCGCC 1 cut(s) 1367
PpsI GAGTC 4 cut(s) 393, 529, 778, 1303
PpuMI RGGWCCY 1 cut(s) 1251
PsiI TTATAA 1 cut(s) 560
Psp5II RGGWCCY 1 cut(s) 1251
Psp6I CCWGG 1 cut(s) 1592
PspGI CCWGG 1 cut(s) 1592
PspN4I GGNNCC 1 cut(s) 1365
PspPI GGNCC 2 cut(s) 36, 1251
PspPPI RGGWCCY 1 cut(s) 1251
PspXI VCTCGAGB 1 cut(s) 767
PsrI GAACNNNNNNTAC 2 cut(s) 780, 812
PvuI CGATCG 1 cut(s) 1020
RruI TCGCGA 1 cut(s) 1016
RseI CAYNNNNRTG 3 cut(s) 227, 425, 530
SaqAI TTAA 3 cut(s) 878, 894, 1475
SatI GCNGC 3 cut(s) 234, 696, 750
Sau3AI GATC 5 cut(s) 85, 286, 762, 1011, 1017
Sau96I GGNCC 2 cut(s) 36, 1251
SchI GAGTC 4 cut(s) 394, 530, 779, 1304
ScrFI CCNGG 1 cut(s) 1594
SfaNI GCATC 3 cut(s) 422, 557, 979
SfoI GGCGCC 1 cut(s) 1365
Sfr274I CTCGAG 1 cut(s) 767
SinI GGWCC 1 cut(s) 1251
SlaI CTCGAG 1 cut(s) 767
SmiMI CAYNNNNRTG 3 cut(s) 227, 425, 530
SmlI CTYRAG 4 cut(s) 506, 605, 767, 1240
SmoI CTYRAG 4 cut(s) 506, 605, 767, 1240
SsiI CCGC 3 cut(s) 197, 233, 696
SspDI GGCGCC 1 cut(s) 1363
SspMI CTAG 2 cut(s) 1157, 1325
StyD4I CCNGG 1 cut(s) 1592
StyI CCWWGG 2 cut(s) 525, 1324
TaaI ACNGT 3 cut(s) 203, 796, 936
TaiI ACGT 1 cut(s) 1266
TauI GCSGC 2 cut(s) 236, 698
TfiI GAWTC 5 cut(s) 143, 264, 674, 942, 1304
Tru1I TTAA 3 cut(s) 878, 894, 1475
Tru9I TTAA 3 cut(s) 878, 894, 1475
TscAI CASTG 4 cut(s) 106, 331, 763, 941
TseFI GTSAC 5 cut(s) 49, 400, 427, 837, 883
TseI GCWGC 1 cut(s) 749
Tsp45I GTSAC 5 cut(s) 49, 400, 427, 837, 883
TspDTI ATGAA 4 cut(s) 32, 109, 309, 788
TspGWI ACGGA 1 cut(s) 669
TspRI CASTG 4 cut(s) 106, 331, 763, 941
VpaK11BI GGWCC 1 cut(s) 1251
XagI CCTNNNNNAGG 1 cut(s) 1328
XapI RAATTY 9 cut(s) 55, 104, 134, 242, 274, 809, 1073, 1145, 1564
XcmI CCANNNNNNNNNTGG 1 cut(s) 1492
XhoI CTCGAG 1 cut(s) 767
XmaJI CCTAGG 1 cut(s) 1324
XmnI GAANNNNTTC 1 cut(s) 828
XspI CTAG 2 cut(s) 1157, 1325
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.