RLG00000024876

Belongs to the GRAS family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0004862
Physical Location & Seq
Forward (+)
39992320 .. 39993921
1602 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000024876

Sequence Viewer

Length: 1602 bp
ATGGAATCTGCATTTTTTGAGCCTTGGTTTTTCGAAAATTTTATAGACTCATCTTTTGACCAACCTGATTTCATCAACATGGATGATACTCCAATTGATAGCCCAGACTTCTCTTCTCTGTTCAACTCAAATGAAGTTTTCAAAATTTCAGAAATTGCTTCAGACCCTTTTGCCACCCTGTTTCCTGGTGATGATCTGATTCAAGTGACATCCCAAGTGGAGGAAGTTTCAATGGATTTGGAAGCATATGAGACTAATTTGATGATAGAGGGTTTCTATGGTAATTCATTGGAAGGGAGTTTTCCATCACAGCAAGCTTCTTCACACCAATATTCTACTGAAGGAAATGATGTGTGGAGTCCAAATTCAGTTGTGAATTCTGAAGCATCATCAATGGACTCAACATCAATCCAATCACCACTAAATTTGCCTAGAGAAGAGGAGGGAATCGAGAACCAATTGAGTATTCGTCATCTACTTCAAGCTTTTGCTGAAGCCAAGGAATATGGCCAGAAAGGGCTAGAGGAGGTTATTCTCAGCTGCATCAGTGAGAAAGTCAGCCCAATTGGAGAGTCTTTCGAGCGCCTTGCATTCAACTTGTGCCAAGAAGTTGTTGATGAACATCAACAAGGCGGCGATTATCTCAAACAAGAATCCTTGAAGAATTTTGAGGCTGCATTTAAGGTGTTCATCCAAAACTTCCCCTATGCTCGGTTTGCTCACTATGCAGCAAACTCAGCAATCCTCGAGGCGATTCCAGCGGAAGCTGAGGCAATTCATATAGTGGATTTCGACATGAGAGAAGGGGTCCAGTTGTCTCAACTTATTCAGGCTTTAGCACAAAGATTCAAAACACTAAAAGTGACAGCAATCAAGTGGGAAGAGGAAGAGAGTGATTGTGCTCCTCCACACGGGAGATTTGAGGAGACAAAGAAGCAACTCCAGAACCATGCTAGATCCTTTGGCTTAAACTTGAAGGTGGAAGAAGTAGCAATGGAGGACTTGGTGACTGAGATAAAGAAGGCAAAGAAGAGGGGTGGAGGGAAAGAATTCTTAGCCTTCAACTGTATGGTTTCACTTCCACACATGAGGAGGAGAAGAAGCAGAGGGCATGTCATGGAGTATCTGAGACTGGCTAAGGAGTTGTTAGCCAATTCTGGGAACTCCAAGACTACTAAAAGTGGTATTATAACTTTTGGTGATGGGGATGCTTGTGAGAGACTTGGAAATCACTCGAGTTTCAGCTCATTCTTTAATGAGAATCTAGTGCATTACCAAGCCTTGTTGGAGTCAATGGAGTCGAATTTCCCAAGTCATCTAGCAGAAGCAAGAATGGTGATGGAATGCATCTTCATAGCACCTTTTGTCTCTTCCCAGGCTTGGTTCCAAAAGTGGACAGAAATGAAGGACTGCCATGTTCAACCATGGTTCGGGCTTGAGGGTCGGAGATTGAGCAAAGAAAATTTGATGGAAGCCAAGGAAATTGTGGGAGATGAAAGATCCTATGGAATCAGGATTGAAGGACTTAATGGGAATGAAATGGCATTGGAATGGAGAGGAACCCCCCTGGTTAGAGTATCAACTTGGGAGAACCAAAGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GRAS

Protein Analysis

534

Amino Acids

60.4

Weight (kDa)

4.76

Isoelectric Point (pI)

49.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRAS PF03514 157 - 530 2e-45 GRAS domain family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1190
AbsI CCTCGAGG 1 cut(s) 746
AciI CCGC 2 cut(s) 633, 761
AclWI GGATC 2 cut(s) 951, 1494
AcoI YGGCCR 1 cut(s) 508
AcsI RAATTY 9 cut(s) 37, 144, 364, 376, 424, 664, 1049, 1303, 1462
AcuI CTGAAG 4 cut(s) 144, 360, 402, 513
AfiI CCNNNNNNNGG 6 cut(s) 220, 711, 911, 1158, 1380, 1430
AjnI CCWGG 3 cut(s) 184, 1374, 1566
AjuI GAANNNNNNNTTGG 2 cut(s) 450, 482
AluBI AGCT 6 cut(s) 317, 485, 540, 767, 1245, 1599
AluI AGCT 6 cut(s) 317, 485, 540, 767, 1245, 1599
Alw21I GWGCWC 1 cut(s) 904
Alw26I GTCTC 6 cut(s) 245, 822, 920, 1123, 1213, 1372
AlwI GGATC 2 cut(s) 951, 1494
Ama87I CYCGRG 2 cut(s) 746, 1234
AoxI GGCC 1 cut(s) 508
ApeKI GCWGC 3 cut(s) 540, 674, 728
ApoI RAATTY 9 cut(s) 37, 144, 364, 376, 424, 664, 1049, 1303, 1462
ArsI GACNNNNNNTTYG 2 cut(s) 38, 70
Asp700I GAANNNNTTC 1 cut(s) 1049
AspLEI GCGC 1 cut(s) 585
AspS9I GGNCC 1 cut(s) 808
AsuHPI GGTGA 5 cut(s) 200, 408, 1018, 1211, 1348
AsuII TTCGAA 1 cut(s) 33
AvaI CYCGRG 2 cut(s) 746, 1234
AvaII GGWCC 1 cut(s) 808
BalI TGGCCA 1 cut(s) 510
Bbv12I GWGCWC 1 cut(s) 904
BbvCI CCTCAGC 1 cut(s) 768
BbvI GCAGC 3 cut(s) 527, 661, 740
BccI CCATC 4 cut(s) 313, 1196, 1333, 1462
BcgI CGANNNNNNTGC 2 cut(s) 569, 603
BciT130I CCWGG 3 cut(s) 186, 1376, 1568
BcoDI GTCTC 6 cut(s) 245, 822, 920, 1123, 1213, 1372
BfaI CTAG 5 cut(s) 432, 521, 954, 1265, 1319
BfoI RGCGCY 1 cut(s) 586
BisI GCNGC 4 cut(s) 541, 634, 675, 729
BlsI GCNGC 4 cut(s) 542, 635, 676, 730
Bme1390I CCNGG 3 cut(s) 186, 1376, 1568
Bme18I GGWCC 1 cut(s) 808
BmeT110I CYCGRG 2 cut(s) 746, 1234
BmgT120I GGNCC 1 cut(s) 808
BmiI GGNNCC 3 cut(s) 809, 1385, 1561
BmrFI CCNGG 3 cut(s) 186, 1376, 1568
BmsI GCATC 4 cut(s) 395, 552, 1198, 1356
BpmI CTGGAG 1 cut(s) 926
Bpu10I CCTNAGC 2 cut(s) 768, 1137
Bpu14I TTCGAA 1 cut(s) 33
BpuEI CTTGAG 1 cut(s) 1457
BsaBI GATNNNNATC 1 cut(s) 621
BsaJI CCNNGG 6 cut(s) 23, 498, 1374, 1424, 1476, 1566
Bsc4I CCNNNNNNNGG 6 cut(s) 220, 711, 911, 1158, 1380, 1430
Bse1I ACTGG 2 cut(s) 811, 1137
Bse3DI GCAATG 1 cut(s) 999
Bse8I GATNNNNATC 1 cut(s) 621
BseBI CCWGG 3 cut(s) 186, 1376, 1568
BseDI CCNNGG 6 cut(s) 23, 498, 1374, 1424, 1476, 1566
BseGI GGATG 4 cut(s) 88, 209, 690, 1213
BseJI GATNNNNATC 1 cut(s) 621
BseLI CCNNNNNNNGG 6 cut(s) 220, 711, 911, 1158, 1380, 1430
BseMI GCAATG 1 cut(s) 999
BseMII CTCAG 5 cut(s) 550, 750, 759, 1002, 1118
BseNI ACTGG 2 cut(s) 811, 1137
BseRI GAGGAG 6 cut(s) 455, 539, 894, 938, 1105, 1108
BseXI GCAGC 3 cut(s) 527, 661, 740
BshFI GGCC 1 cut(s) 510
BsiHKAI GWGCWC 1 cut(s) 904
BsiHKCI CYCGRG 2 cut(s) 746, 1234
BslI CCNNNNNNNGG 6 cut(s) 220, 711, 911, 1158, 1380, 1430
BsmAI GTCTC 6 cut(s) 245, 822, 920, 1123, 1213, 1372
BsmI GAATGC 2 cut(s) 590, 1349
BsnI GGCC 1 cut(s) 510
BsoBI CYCGRG 2 cut(s) 746, 1234
Bsp119I TTCGAA 1 cut(s) 33
Bsp1286I GDGCHC 1 cut(s) 904
Bsp143I GATC 3 cut(s) 193, 956, 1499
Bsp19I CCATGG 1 cut(s) 1424
BspACI CCGC 2 cut(s) 633, 761
BspANI GGCC 1 cut(s) 510
BspCNI CTCAG 5 cut(s) 549, 749, 760, 1003, 1119
BspLI GGNNCC 3 cut(s) 809, 1385, 1561
BspPI GGATC 2 cut(s) 951, 1494
BspT104I TTCGAA 1 cut(s) 33
BsrDI GCAATG 1 cut(s) 999
BsrI ACTGG 2 cut(s) 811, 1137
BssECI CCNNGG 6 cut(s) 23, 498, 1374, 1424, 1476, 1566
BssMI GATC 3 cut(s) 193, 956, 1499
BssT1I CCWWGG 4 cut(s) 23, 498, 1424, 1476
Bst2UI CCWGG 3 cut(s) 186, 1376, 1568
Bst4CI ACNGT 1 cut(s) 1067
Bst6I CTCTTC 6 cut(s) 118, 432, 876, 882, 1025, 1375
BstBI TTCGAA 1 cut(s) 33
BstC8I GCNNGC 1 cut(s) 315
BstDEI CTNAG 7 cut(s) 536, 736, 768, 1011, 1054, 1127, 1137
BstDSI CCRYGG 1 cut(s) 1424
BstF5I GGATG 4 cut(s) 88, 209, 690, 1213
BstH2I RGCGCY 1 cut(s) 586
BstHHI GCGC 1 cut(s) 585
BstKTI GATC 3 cut(s) 196, 959, 1502
BstMAI GTCTC 6 cut(s) 245, 822, 920, 1123, 1213, 1372
BstMBI GATC 3 cut(s) 193, 956, 1499
BstMWI GCNNNNNNNGC 4 cut(s) 716, 725, 737, 758
BstNI CCWGG 3 cut(s) 186, 1376, 1568
BstNSI RCATGY 1 cut(s) 1115
BstSCI CCNGG 3 cut(s) 184, 1374, 1566
BstV1I GCAGC 3 cut(s) 527, 661, 740
BstX2I RGATCY 2 cut(s) 956, 1499
BstYI RGATCY 2 cut(s) 956, 1499
BsuRI GGCC 1 cut(s) 510
BtgI CCRYGG 1 cut(s) 1424
BtsCI GGATG 4 cut(s) 88, 209, 690, 1213
BtsIMutI CAGTG 1 cut(s) 553
Cac8I GCNNGC 1 cut(s) 315
CfoI GCGC 1 cut(s) 585
Cfr13I GGNCC 1 cut(s) 808
CspCI CAANNNNNGTGG 2 cut(s) 408, 443
CviAII CATG 8 cut(s) 79, 796, 950, 1087, 1112, 1117, 1415, 1425
DdeI CTNAG 7 cut(s) 536, 736, 768, 1011, 1054, 1127, 1137
DpnI GATC 3 cut(s) 195, 958, 1501
DpnII GATC 3 cut(s) 193, 956, 1499
EaeI YGGCCR 1 cut(s) 508
Eam1104I CTCTTC 6 cut(s) 118, 432, 876, 882, 1025, 1375
EarI CTCTTC 6 cut(s) 118, 432, 876, 882, 1025, 1375
Eco130I CCWWGG 4 cut(s) 23, 498, 1424, 1476
Eco47I GGWCC 1 cut(s) 808
Eco57I CTGAAG 4 cut(s) 144, 360, 402, 513
Eco88I CYCGRG 2 cut(s) 746, 1234
EcoRI GAATTC 2 cut(s) 376, 1049
EcoRII CCWGG 3 cut(s) 184, 1374, 1566
EcoT14I CCWWGG 4 cut(s) 23, 498, 1424, 1476
EcoT22I ATGCAT 1 cut(s) 1349
ErhI CCWWGG 4 cut(s) 23, 498, 1424, 1476
FaeI CATG 8 cut(s) 82, 799, 953, 1090, 1115, 1120, 1418, 1428
FatI CATG 8 cut(s) 78, 795, 949, 1086, 1111, 1116, 1414, 1424
FauNDI CATATG 1 cut(s) 247
Fnu4HI GCNGC 4 cut(s) 541, 634, 675, 729
FokI GGATG 4 cut(s) 95, 196, 677, 1220
Fsp4HI GCNGC 4 cut(s) 541, 634, 675, 729
FspBI CTAG 5 cut(s) 432, 521, 954, 1265, 1319
GlaI GCGC 1 cut(s) 584
GluI GCNGC 4 cut(s) 541, 634, 675, 729
GsuI CTGGAG 1 cut(s) 926
HaeII RGCGCY 1 cut(s) 586
HaeIII GGCC 1 cut(s) 510
HhaI GCGC 1 cut(s) 585
Hin1II CATG 8 cut(s) 82, 799, 953, 1090, 1115, 1120, 1418, 1428
Hin6I GCGC 1 cut(s) 583
HinP1I GCGC 1 cut(s) 583
HindIII AAGCTT 2 cut(s) 315, 483
HphI GGTGA 5 cut(s) 200, 408, 1018, 1211, 1348
Hpy166II GTNNAC 1 cut(s) 1395
Hpy188I TCNGA 6 cut(s) 151, 163, 198, 382, 1128, 1446
Hpy188III TCNNGA 3 cut(s) 451, 943, 1513
Hpy8I GTNNAC 1 cut(s) 1395
HpyAV CCTTC 8 cut(s) 287, 335, 797, 970, 1015, 1069, 1399, 1514
HpyCH4III ACNGT 1 cut(s) 1067
HpyCH4V TGCA 7 cut(s) 11, 543, 590, 677, 728, 1270, 1347
HpyF10VI GCNNNNNNNGC 4 cut(s) 716, 725, 737, 758
HpyF3I CTNAG 7 cut(s) 536, 736, 768, 1011, 1054, 1127, 1137
Hsp92II CATG 8 cut(s) 82, 799, 953, 1090, 1115, 1120, 1418, 1428
HspAI GCGC 1 cut(s) 583
Kzo9I GATC 3 cut(s) 193, 956, 1499
LmnI GCTCC 1 cut(s) 907
Lsp1109I GCAGC 3 cut(s) 527, 661, 740
LweI GCATC 4 cut(s) 395, 552, 1198, 1356
MaeI CTAG 5 cut(s) 432, 521, 954, 1265, 1319
MaeIII GTNAC 3 cut(s) 205, 862, 1006
MalI GATC 3 cut(s) 195, 958, 1501
MboI GATC 3 cut(s) 193, 956, 1499
MfeI CAATTG 3 cut(s) 93, 458, 564
MflI RGATCY 2 cut(s) 956, 1499
MhlI GDGCHC 1 cut(s) 904
MlsI TGGCCA 1 cut(s) 510
MluNI TGGCCA 1 cut(s) 510
MlyI GAGTC 6 cut(s) 41, 367, 392, 581, 1298, 1307
MmeI TCCRAC 2 cut(s) 1266, 1424
Mox20I TGGCCA 1 cut(s) 510
Mph1103I ATGCAT 1 cut(s) 1349
MroXI GAANNNNTTC 1 cut(s) 1049
MscI TGGCCA 1 cut(s) 510
MseI TTAA 4 cut(s) 681, 968, 1254, 1527
MslI CAYNNNNRTG 2 cut(s) 77, 1549
Msp20I TGGCCA 1 cut(s) 510
MspA1I CMGCKG 2 cut(s) 540, 761
MspR9I CCNGG 3 cut(s) 186, 1376, 1568
MunI CAATTG 3 cut(s) 93, 458, 564
Mva1269I GAATGC 2 cut(s) 590, 1349
MvaI CCWGG 3 cut(s) 186, 1376, 1568
MwoI GCNNNNNNNGC 4 cut(s) 716, 725, 737, 758
NcoI CCATGG 1 cut(s) 1424
NdeI CATATG 1 cut(s) 247
NdeII GATC 3 cut(s) 193, 956, 1499
NlaIII CATG 8 cut(s) 82, 799, 953, 1090, 1115, 1120, 1418, 1428
NlaIV GGNNCC 3 cut(s) 809, 1385, 1561
NmuCI GTSAC 3 cut(s) 205, 862, 1006
NsiI ATGCAT 1 cut(s) 1349
NspI RCATGY 1 cut(s) 1115
NspV TTCGAA 1 cut(s) 33
PaeR7I CTCGAG 2 cut(s) 746, 1234
PctI GAATGC 2 cut(s) 590, 1349
PdmI GAANNNNTTC 1 cut(s) 1049
PfeI GAWTC 8 cut(s) 5, 199, 447, 653, 754, 846, 1261, 1509
PkrI GCNGC 4 cut(s) 542, 635, 676, 730
PleI GAGTC 6 cut(s) 41, 366, 392, 580, 1297, 1306
PpsI GAGTC 6 cut(s) 41, 366, 392, 580, 1297, 1306
PsiI TTATAA 1 cut(s) 1190
Psp6I CCWGG 3 cut(s) 184, 1374, 1566
PspGI CCWGG 3 cut(s) 184, 1374, 1566
PspN4I GGNNCC 3 cut(s) 809, 1385, 1561
PspPI GGNCC 1 cut(s) 808
PspXI VCTCGAGB 2 cut(s) 746, 1234
PsuI RGATCY 2 cut(s) 956, 1499
PvuII CAGCTG 1 cut(s) 540
RseI CAYNNNNRTG 2 cut(s) 77, 1549
SaqAI TTAA 4 cut(s) 681, 968, 1254, 1527
SatI GCNGC 4 cut(s) 541, 634, 675, 729
Sau3AI GATC 3 cut(s) 193, 956, 1499
Sau96I GGNCC 1 cut(s) 808
SchI GAGTC 6 cut(s) 41, 367, 392, 581, 1298, 1307
ScrFI CCNGG 3 cut(s) 186, 1376, 1568
SduI GDGCHC 1 cut(s) 904
SfaNI GCATC 4 cut(s) 395, 552, 1198, 1356
Sfr274I CTCGAG 2 cut(s) 746, 1234
SfuI TTCGAA 1 cut(s) 33
SinI GGWCC 1 cut(s) 808
SlaI CTCGAG 2 cut(s) 746, 1234
SmiMI CAYNNNNRTG 2 cut(s) 77, 1549
SmlI CTYRAG 3 cut(s) 746, 1234, 1436
SmoI CTYRAG 3 cut(s) 746, 1234, 1436
SsiI CCGC 2 cut(s) 633, 761
SspI AATATT 1 cut(s) 332
SspMI CTAG 5 cut(s) 432, 521, 954, 1265, 1319
StyD4I CCNGG 3 cut(s) 184, 1374, 1566
StyI CCWWGG 4 cut(s) 23, 498, 1424, 1476
TaaI ACNGT 1 cut(s) 1067
TaqI TCGA 7 cut(s) 33, 450, 579, 747, 792, 1235, 1301
TauI GCSGC 1 cut(s) 636
TfiI GAWTC 8 cut(s) 5, 199, 447, 653, 754, 846, 1261, 1509
Tru1I TTAA 4 cut(s) 681, 968, 1254, 1527
Tru9I TTAA 4 cut(s) 681, 968, 1254, 1527
TscAI CASTG 1 cut(s) 553
TseFI GTSAC 3 cut(s) 205, 862, 1006
TseI GCWGC 3 cut(s) 540, 674, 728
Tsp45I GTSAC 3 cut(s) 205, 862, 1006
TspRI CASTG 1 cut(s) 553
VpaK11BI GGWCC 1 cut(s) 808
XapI RAATTY 9 cut(s) 37, 144, 364, 376, 424, 664, 1049, 1303, 1462
XceI RCATGY 1 cut(s) 1115
XcmI CCANNNNNNNNNTGG 1 cut(s) 1483
XhoI CTCGAG 2 cut(s) 746, 1234
XmnI GAANNNNTTC 1 cut(s) 1049
XspI CTAG 5 cut(s) 432, 521, 954, 1265, 1319
Zsp2I ATGCAT 1 cut(s) 1349
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.