MD12G1177000.v1.1

Belongs to the GRAS family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0004862
Physical Location & Seq
Forward (+)
25740719 .. 25742332
1614 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1177000.v1.1.491

Sequence Viewer

Length: 1614 bp
ATGATGCAATCAGAGATGAACTTTCAGACCTTCTGGCCATGCCTGAATGAAACAAATTCAACTTTGGATACAAATGAATACACTGAGCAGATGGGGAGCTCTGAATTTTCTTCGATTTTCATGCCATCGGACGAATTTTCAGAAACTTCTAATTCTTTTCCATTTTCAACCATGTTTTCTGGTGAATCTGAACAACTTGCAGATTGTGATCATCTCCTGCAAGCCACATGGATGGCGGAAGAGGAATTTACCATAGAGTTGGGAGGATTCGAGACGAATTATTTGATGGGTGAGATAGAAAATATGTATGCCTCATGTGTGGAAGGCAGTGAGGGAAGCAATACTCTTCCTTCACCGCAATTTTCTGTTGAAGGAAATGGTGTGTGGAGTCCAAACTCTTTTGTGACTTCTGAGGCATCCTCCATGGATGTGACATCCGTCCAACAATCAATAACCCTGGCGAGAGACGAGATGGAAATCAACAGCGAAGTGAGAATTCGCCATATGCTCAAGGCGTTCAACGAGGCCATGGAGATGGGGCAGAGGAAGCTAGAGGAGGTAATCATGAGATTCCTTGCGGAGAAAGTAAACCCACTTGGTCAATCTCTTGAGCGCCTTGCATTCTACTTATGTCTAGGATTTGTTGATAATCAGCAGGGAGACTATTTGAAACAAGAATCCTTCAAGAATTTTGAGGACGCTTTCAACATGTTCTACCAAATCTTCCCTTTTGGTAGATTTGCTCACTACGCAGCAAACACCGCGATCCTCGAGGCTGTCCCGGAGGATGTAGAGACGGTTCATGTAGTGGAATTTGATATCGGTGAAGGGGTTCAATTGCCTCAGCTGATTGAGGCTGTGGCAAAGAGAAACAAAACACTTAAAGTGACAGCAATTAAATGGGATGTGGAAGAGAGTGATGAGGTTGCTCCTCCACAGTGGAGATTTGAGGAGACGAAAAGGCAACTCCAGCATCACGCAAGGTCATTCGGGCTAAACTTGAAGGTGGAGGAGATCCCGATTGAGGATTTGGTGAGCAAAATCAAAAAGGTGAACAAGAGAGGGGGAATGAGAGAATTTCTAGCCTTCAACTCTATGGTGGGGCTTCCTCATATGAGGAGGAGAAGAAGCAGAGGACTTCTCATGGAATTCCTAAGGCTAGCTAAGGATTTGTTAACCAATTCGGCAAGAGGTATCATAACTTTTGGTGACGGAGATGCTTACGCGAGAAGGGGAAATGACTCGAGTTTCATCTCCTTCTTTGACGCGAATGTTGTGCACTACGAAGCCTTGTTAGGGTCTTTGGAATCGAGCTTCCCAAGCCATCTAGGAGAGGCAAGGATGGTGATGGAGTTGATGTTTGTGGCGCCGTATGTGTCTTCTCAGGCTTGGTTCGAGAAGTGGAACGAAGCAAGAGAAGCTGGAAATCTTCATCCCTGGTTCGGGCTGGAGGGTAGGAGAGTGAGCAGGGAAATGTTAATGGAGGCCAAAGAAATTGTGGGAGCAGATAGCTCGTATGGAGTGAGAGTTGGAGGAGAGAATGGGAATGAGATGACATTGGAATGGAATTCAATTCCTTTGATCAGAGTTGAAACCTGGACAAACCGAAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GRAS

Protein Analysis

538

Amino Acids

61.17

Weight (kDa)

4.74

Isoelectric Point (pI)

51.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRAS PF03514 167 - 534 2.2e-39 GRAS domain family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 770
AccB1I GGYRCC 1 cut(s) 1366
AccII CGCG 3 cut(s) 764, 1226, 1268
AciI CCGC 4 cut(s) 236, 356, 578, 762
AclWI GGATC 2 cut(s) 760, 1009
AcoI YGGCCR 1 cut(s) 35
AcyI GRCGYC 1 cut(s) 1367
AfiI CCNNNNNNNGG 4 cut(s) 1024, 1296, 1442, 1443
AflIII ACRYGT 1 cut(s) 708
AjnI CCWGG 3 cut(s) 456, 1436, 1595
AluBI AGCT 8 cut(s) 99, 550, 847, 1163, 1314, 1421, 1512, 1611
AluI AGCT 8 cut(s) 99, 550, 847, 1163, 1314, 1421, 1512, 1611
Alw21I GWGCWC 2 cut(s) 101, 1281
Alw26I GTCTC 5 cut(s) 266, 459, 654, 788, 947
Alw44I GTGCAC 1 cut(s) 1277
AlwI GGATC 2 cut(s) 760, 1009
Ama87I CYCGRG 2 cut(s) 770, 1243
AoxI GGCC 3 cut(s) 35, 525, 1485
ApaLI GTGCAC 1 cut(s) 1277
ApeKI GCWGC 1 cut(s) 752
ArsI GACNNNNNNTTYG 2 cut(s) 265, 297
Asp700I GAANNNNTTC 1 cut(s) 831
AspLEI GCGC 2 cut(s) 615, 1369
AsuC2I CCSGG 1 cut(s) 782
AsuHPI GGTGA 8 cut(s) 194, 302, 345, 836, 1045, 1063, 1220, 1357
AsuNHI GCTAGC 1 cut(s) 1159
AvaI CYCGRG 2 cut(s) 770, 1243
AxyI CCTNAGG 1 cut(s) 1154
BaeGI GKGCMC 1 cut(s) 1281
BalI TGGCCA 1 cut(s) 37
BanI GGYRCC 1 cut(s) 1366
BanII GRGCYC 1 cut(s) 101
BbsI GAAGAC 1 cut(s) 1371
Bbv12I GWGCWC 2 cut(s) 101, 1281
BbvCI CCTCAGC 1 cut(s) 843
BbvI GCAGC 1 cut(s) 764
BccI CCATC 9 cut(s) 85, 133, 226, 280, 466, 529, 1332, 1336, 1342
BceAI ACGGC 1 cut(s) 1354
BcgI CGANNNNNNTGC 6 cut(s) 103, 137, 1258, 1292, 1494, 1528
BciT130I CCWGG 3 cut(s) 458, 1438, 1597
BciVI GTATCC 1 cut(s) 61
BclI TGATCA 2 cut(s) 208, 1581
BcnI CCSGG 1 cut(s) 782
BcoDI GTCTC 5 cut(s) 266, 459, 654, 788, 947
BfaI CTAG 6 cut(s) 551, 635, 1082, 1160, 1328, 1612
BfoI RGCGCY 2 cut(s) 616, 1370
BfuI GTATCC 1 cut(s) 61
BisI GCNGC 1 cut(s) 753
BlsI GCNGC 1 cut(s) 754
Bme1390I CCNGG 4 cut(s) 458, 782, 1438, 1597
BmeT110I CYCGRG 2 cut(s) 770, 1243
BmiI GGNNCC 1 cut(s) 1368
BmrFI CCNGG 4 cut(s) 458, 782, 1438, 1597
BmsI GCATC 3 cut(s) 425, 982, 1207
BmtI GCTAGC 1 cut(s) 1163
BoxI GACNNNNGTC 1 cut(s) 437
BpiI GAAGAC 1 cut(s) 1371
BplI GAGNNNNNCTC 4 cut(s) 404, 436, 1125, 1157
BpmI CTGGAG 2 cut(s) 953, 1469
Bpu10I CCTNAGC 2 cut(s) 843, 1164
BpuEI CTTGAG 2 cut(s) 494, 629
BpuMI CCSGG 1 cut(s) 782
BsaBI GATNNNNATC 2 cut(s) 207, 476
BsaHI GRCGYC 1 cut(s) 1367
BsaJI CCNNGG 4 cut(s) 423, 456, 528, 1436
BsaXI ACNNNNNCTCC 4 cut(s) 1343, 1373, 1512, 1542
Bsc4I CCNNNNNNNGG 4 cut(s) 1024, 1296, 1442, 1443
Bse21I CCTNAGG 1 cut(s) 1154
Bse8I GATNNNNATC 2 cut(s) 207, 476
BseBI CCWGG 3 cut(s) 458, 1438, 1597
BseDI CCNNGG 4 cut(s) 423, 456, 528, 1436
BseGI GGATG 8 cut(s) 237, 416, 433, 434, 793, 910, 1347, 1432
BseJI GATNNNNATC 2 cut(s) 207, 476
BseLI CCNNNNNNNGG 4 cut(s) 1024, 1296, 1442, 1443
BseMII CTCAG 4 cut(s) 75, 402, 857, 1397
BseRI GAGGAG 7 cut(s) 569, 921, 965, 1025, 1132, 1135, 1548
BseSI GKGCMC 1 cut(s) 1281
BseXI GCAGC 1 cut(s) 764
Bsh1236I CGCG 3 cut(s) 764, 1226, 1268
BshFI GGCC 3 cut(s) 37, 527, 1487
BshNI GGYRCC 1 cut(s) 1366
BsiHKAI GWGCWC 2 cut(s) 101, 1281
BsiHKCI CYCGRG 2 cut(s) 770, 1243
BsiSI CCGG 1 cut(s) 782
BslFI GGGAC 1 cut(s) 764
BslI CCNNNNNNNGG 4 cut(s) 1024, 1296, 1442, 1443
BsmAI GTCTC 5 cut(s) 266, 459, 654, 788, 947
BsmBI CGTCTC 4 cut(s) 266, 459, 788, 947
BsmFI GGGAC 1 cut(s) 764
BsmI GAATGC 1 cut(s) 620
BsnI GGCC 3 cut(s) 37, 527, 1487
BsoBI CYCGRG 2 cut(s) 770, 1243
Bsp1286I GDGCHC 2 cut(s) 101, 1281
Bsp143I GATC 4 cut(s) 208, 765, 1014, 1581
Bsp19I CCATGG 2 cut(s) 423, 528
BspACI CCGC 4 cut(s) 236, 356, 578, 762
BspANI GGCC 3 cut(s) 37, 527, 1487
BspCNI CTCAG 4 cut(s) 76, 403, 856, 1396
BspFNI CGCG 3 cut(s) 764, 1226, 1268
BspHI TCATGA 1 cut(s) 564
BspLI GGNNCC 1 cut(s) 1368
BspOI GCTAGC 1 cut(s) 1163
BspPI GGATC 2 cut(s) 760, 1009
BspT107I GGYRCC 1 cut(s) 1366
BssECI CCNNGG 4 cut(s) 423, 456, 528, 1436
BssMI GATC 4 cut(s) 208, 765, 1014, 1581
BssNI GRCGYC 1 cut(s) 1367
BssT1I CCWWGG 2 cut(s) 423, 528
Bst2UI CCWGG 3 cut(s) 458, 1438, 1597
Bst4CI ACNGT 2 cut(s) 799, 939
Bst6I CTCTTC 3 cut(s) 234, 351, 906
BstACI GRCGYC 1 cut(s) 1367
BstC8I GCNNGC 2 cut(s) 222, 1161
BstDEI CTNAG 6 cut(s) 84, 411, 843, 1154, 1164, 1383
BstDSI CCRYGG 2 cut(s) 423, 528
BstF5I GGATG 8 cut(s) 237, 416, 433, 434, 793, 910, 1347, 1432
BstFNI CGCG 3 cut(s) 764, 1226, 1268
BstH2I RGCGCY 2 cut(s) 616, 1370
BstHHI GCGC 2 cut(s) 615, 1369
BstKTI GATC 4 cut(s) 211, 768, 1017, 1584
BstMAI GTCTC 5 cut(s) 266, 459, 654, 788, 947
BstMBI GATC 4 cut(s) 208, 765, 1014, 1581
BstMWI GCNNNNNNNGC 6 cut(s) 547, 749, 761, 970, 1320, 1418
BstNI CCWGG 3 cut(s) 458, 1438, 1597
BstNSI RCATGY 1 cut(s) 712
BstPAI GACNNNNGTC 1 cut(s) 437
BstSCI CCNGG 4 cut(s) 456, 780, 1436, 1595
BstSLI GKGCMC 1 cut(s) 1281
BstUI CGCG 3 cut(s) 764, 1226, 1268
BstV1I GCAGC 1 cut(s) 764
BstV2I GAAGAC 1 cut(s) 1371
BstX2I RGATCY 1 cut(s) 1014
BstXI CCANNNNNNTGG 3 cut(s) 232, 259, 535
BstYI RGATCY 1 cut(s) 1014
Bsu36I CCTNAGG 1 cut(s) 1154
BsuI GTATCC 1 cut(s) 61
BsuRI GGCC 3 cut(s) 37, 527, 1487
BtgI CCRYGG 2 cut(s) 423, 528
BtsCI GGATG 8 cut(s) 237, 416, 433, 434, 793, 910, 1347, 1432
BtsI GCAGTG 1 cut(s) 334
BtsIMutI CAGTG 3 cut(s) 81, 334, 944
Cac8I GCNNGC 2 cut(s) 222, 1161
CciI TCATGA 1 cut(s) 564
CfoI GCGC 2 cut(s) 615, 1369
CseI GACGC 2 cut(s) 707, 1274
DdeI CTNAG 6 cut(s) 84, 411, 843, 1154, 1164, 1383
DinI GGCGCC 1 cut(s) 1368
DpnI GATC 4 cut(s) 210, 767, 1016, 1583
DpnII GATC 4 cut(s) 208, 765, 1014, 1581
EaeI YGGCCR 1 cut(s) 35
Eam1104I CTCTTC 3 cut(s) 234, 351, 906
EarI CTCTTC 3 cut(s) 234, 351, 906
EciI GGCGGA 1 cut(s) 251
Ecl136II GAGCTC 1 cut(s) 99
Eco130I CCWWGG 2 cut(s) 423, 528
Eco24I GRGCYC 1 cut(s) 101
Eco32I GATATC 1 cut(s) 820
Eco53kI GAGCTC 1 cut(s) 99
Eco81I CCTNAGG 1 cut(s) 1154
Eco88I CYCGRG 2 cut(s) 770, 1243
EcoICRI GAGCTC 1 cut(s) 99
EcoRI GAATTC 3 cut(s) 495, 1148, 1567
EcoRII CCWGG 3 cut(s) 456, 1436, 1595
EcoRV GATATC 1 cut(s) 820
EcoT14I CCWWGG 2 cut(s) 423, 528
EcoT38I GRGCYC 1 cut(s) 101
EgeI GGCGCC 1 cut(s) 1368
EheI GGCGCC 1 cut(s) 1368
ErhI CCWWGG 2 cut(s) 423, 528
Esp3I CGTCTC 4 cut(s) 266, 459, 788, 947
FaqI GGGAC 1 cut(s) 764
FauNDI CATATG 2 cut(s) 504, 1113
FbaI TGATCA 2 cut(s) 208, 1581
Fnu4HI GCNGC 1 cut(s) 753
FokI GGATG 8 cut(s) 244, 403, 421, 440, 800, 917, 1354, 1419
FriOI GRGCYC 1 cut(s) 101
Fsp4HI GCNGC 1 cut(s) 753
FspBI CTAG 6 cut(s) 551, 635, 1082, 1160, 1328, 1612
GlaI GCGC 2 cut(s) 614, 1368
GluI GCNGC 1 cut(s) 753
GsuI CTGGAG 2 cut(s) 953, 1469
HaeII RGCGCY 2 cut(s) 616, 1370
HaeIII GGCC 3 cut(s) 37, 527, 1487
HapII CCGG 1 cut(s) 782
HgaI GACGC 2 cut(s) 707, 1274
HhaI GCGC 2 cut(s) 615, 1369
Hin1I GRCGYC 1 cut(s) 1367
Hin6I GCGC 2 cut(s) 613, 1367
HinP1I GCGC 2 cut(s) 613, 1367
HincII GTYRAC 1 cut(s) 1176
HindII GTYRAC 1 cut(s) 1176
HinfI GANTC 7 cut(s) 185, 267, 388, 570, 677, 1241, 1307
HpaI GTTAAC 1 cut(s) 1176
HpaII CCGG 1 cut(s) 782
HphI GGTGA 8 cut(s) 194, 302, 345, 836, 1045, 1063, 1220, 1357
Hpy166II GTNNAC 4 cut(s) 589, 1054, 1176, 1279
Hpy188I TCNGA 8 cut(s) 13, 27, 103, 130, 142, 190, 412, 1586
Hpy188III TCNNGA 6 cut(s) 271, 565, 608, 685, 1018, 1396
Hpy8I GTNNAC 4 cut(s) 589, 1054, 1176, 1279
HpyCH4III ACNGT 2 cut(s) 799, 939
HpyCH4V TGCA 5 cut(s) 7, 200, 220, 620, 1279
HpyF10VI GCNNNNNNNGC 6 cut(s) 547, 749, 761, 970, 1320, 1418
HpyF3I CTNAG 6 cut(s) 84, 411, 843, 1154, 1164, 1383
Hsp92I GRCGYC 1 cut(s) 1367
HspAI GCGC 2 cut(s) 613, 1367
KasI GGCGCC 1 cut(s) 1366
Ksp22I TGATCA 2 cut(s) 208, 1581
KspAI GTTAAC 1 cut(s) 1176
Kzo9I GATC 4 cut(s) 208, 765, 1014, 1581
LmnI GCTCC 3 cut(s) 96, 934, 1502
Lsp1109I GCAGC 1 cut(s) 764
LweI GCATC 3 cut(s) 425, 982, 1207
MaeI CTAG 6 cut(s) 551, 635, 1082, 1160, 1328, 1612
MaeIII GTNAC 4 cut(s) 403, 430, 886, 1208
MalI GATC 4 cut(s) 210, 767, 1016, 1583
MboI GATC 4 cut(s) 208, 765, 1014, 1581
MboII GAAGA 8 cut(s) 102, 251, 338, 715, 923, 1137, 1371, 1421
MfeI CAATTG 1 cut(s) 836
MflI RGATCY 1 cut(s) 1014
MhlI GDGCHC 2 cut(s) 101, 1281
MlsI TGGCCA 1 cut(s) 37
MluNI TGGCCA 1 cut(s) 37
Mly113I GGCGCC 1 cut(s) 1367
MlyI GAGTC 2 cut(s) 397, 1235
MmeI TCCRAC 2 cut(s) 466, 1510
Mox20I TGGCCA 1 cut(s) 37
MroXI GAANNNNTTC 1 cut(s) 831
MscI TGGCCA 1 cut(s) 37
MseI TTAA 4 cut(s) 882, 897, 1175, 1478
MslI CAYNNNNRTG 4 cut(s) 230, 428, 533, 1561
Msp20I TGGCCA 1 cut(s) 37
MspA1I CMGCKG 1 cut(s) 847
MspI CCGG 1 cut(s) 782
MspR9I CCNGG 4 cut(s) 458, 782, 1438, 1597
MunI CAATTG 1 cut(s) 836
Mva1269I GAATGC 1 cut(s) 620
MvaI CCWGG 3 cut(s) 458, 1438, 1597
MvnI CGCG 3 cut(s) 764, 1226, 1268
MwoI GCNNNNNNNGC 6 cut(s) 547, 749, 761, 970, 1320, 1418
NarI GGCGCC 1 cut(s) 1367
NciI CCSGG 1 cut(s) 782
NcoI CCATGG 2 cut(s) 423, 528
NdeI CATATG 2 cut(s) 504, 1113
NdeII GATC 4 cut(s) 208, 765, 1014, 1581
NheI GCTAGC 1 cut(s) 1159
NlaIV GGNNCC 1 cut(s) 1368
NmuCI GTSAC 4 cut(s) 403, 430, 886, 1208
NspI RCATGY 1 cut(s) 712
PaeR7I CTCGAG 2 cut(s) 770, 1243
PagI TCATGA 1 cut(s) 564
PciI ACATGT 1 cut(s) 708
PctI GAATGC 1 cut(s) 620
PdmI GAANNNNTTC 1 cut(s) 831
PfeI GAWTC 5 cut(s) 185, 267, 570, 677, 1307
PfoI TCCNGGA 1 cut(s) 780
PkrI GCNGC 1 cut(s) 754
PleI GAGTC 2 cut(s) 396, 1235
PluTI GGCGCC 1 cut(s) 1370
PpsI GAGTC 2 cut(s) 396, 1235
PscI ACATGT 1 cut(s) 708
PshAI GACNNNNGTC 1 cut(s) 437
Psp124BI GAGCTC 1 cut(s) 101
Psp6I CCWGG 3 cut(s) 456, 1436, 1595
PspGI CCWGG 3 cut(s) 456, 1436, 1595
PspN4I GGNNCC 1 cut(s) 1368
PspXI VCTCGAGB 2 cut(s) 770, 1243
PsrI GAACNNNNNNTAC 2 cut(s) 783, 815
PsuI RGATCY 1 cut(s) 1014
PvuII CAGCTG 1 cut(s) 847
RseI CAYNNNNRTG 4 cut(s) 230, 428, 533, 1561
SacI GAGCTC 1 cut(s) 101
SaqAI TTAA 4 cut(s) 882, 897, 1175, 1478
SatI GCNGC 1 cut(s) 753
Sau3AI GATC 4 cut(s) 208, 765, 1014, 1581
SchI GAGTC 2 cut(s) 397, 1235
ScrFI CCNGG 4 cut(s) 458, 782, 1438, 1597
SduI GDGCHC 2 cut(s) 101, 1281
SfaNI GCATC 3 cut(s) 425, 982, 1207
SfoI GGCGCC 1 cut(s) 1368
Sfr274I CTCGAG 2 cut(s) 770, 1243
SlaI CTCGAG 2 cut(s) 770, 1243
SmiMI CAYNNNNRTG 4 cut(s) 230, 428, 533, 1561
SmlI CTYRAG 4 cut(s) 509, 608, 770, 1243
SmoI CTYRAG 4 cut(s) 509, 608, 770, 1243
SsiI CCGC 4 cut(s) 236, 356, 578, 762
SspDI GGCGCC 1 cut(s) 1366
SspMI CTAG 6 cut(s) 551, 635, 1082, 1160, 1328, 1612
SstI GAGCTC 1 cut(s) 101
StyD4I CCNGG 4 cut(s) 456, 780, 1436, 1595
StyI CCWWGG 2 cut(s) 423, 528
TaaI ACNGT 2 cut(s) 799, 939
TaqI TCGA 6 cut(s) 113, 270, 771, 1244, 1310, 1395
TfiI GAWTC 5 cut(s) 185, 267, 570, 677, 1307
Tru1I TTAA 4 cut(s) 882, 897, 1175, 1478
Tru9I TTAA 4 cut(s) 882, 897, 1175, 1478
TscAI CASTG 3 cut(s) 88, 334, 944
TseFI GTSAC 4 cut(s) 403, 430, 886, 1208
TseI GCWGC 1 cut(s) 752
Tsp45I GTSAC 4 cut(s) 403, 430, 886, 1208
TspDTI ATGAA 7 cut(s) 32, 63, 90, 109, 791, 1240, 1421
TspGWI ACGGA 2 cut(s) 427, 1227
TspRI CASTG 3 cut(s) 88, 334, 944
VneI GTGCAC 1 cut(s) 1277
XceI RCATGY 1 cut(s) 712
XcmI CCANNNNNNNNNTGG 1 cut(s) 1495
XhoI CTCGAG 2 cut(s) 770, 1243
XmnI GAANNNNTTC 1 cut(s) 831
XspI CTAG 6 cut(s) 551, 635, 1082, 1160, 1328, 1612
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.