MD12G1177200.v1.1

Belongs to the GRAS family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0004862
Physical Location & Seq
Forward (+)
25763298 .. 25764440
1143 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1177200.v1.1.491

Sequence Viewer

Length: 1143 bp
ATGGAAATCGACAACGAAGTGAGTATTCGCCATCTGCTCAAGGCGTTCGGCGAGGCAATGGAGATGGGGCAGAGGGAGCTGGAGGAGGTGATCATGAGATGCCTTGCGGAGAAAGTAAACCCACTTGGTCAATCTCTTGAGCGCCTTGCATTCTACTTATGTCAAGGAGTGGTTGATAATCAGCAGGGAGACTATTTGAAACAAGAATCCTGCAAGAATTTTGAGGCCGCTTTCAACTTGTTCTACCAAATCTTCCCTTTTGGGAGATTTGCTCACTACGCAGCAAACACCGCAATCCTCGAGGCTGTCCCGGAGGATGTCGAGACGGTTCATGTAGTGGAATTTGATATCGGTGAAGGGGTTCAATTGTCTCAGCTGATTGAGTCTGTGGCAAAGAGAAATAAAACACTTGAAGTGACAGCAATTAAATGGGATGTGGAAGAGAGTGATGAGGCTGCTCCTCCACAGTGGAGATTCAAGGAGACAAAAAGGCAACTCCAACATCATGCAAGGTCTTTCGGGGTAAACTTGAAGGTGGAGGAGATCGCGATTGAGGATTTGGTGAGCGAAATCAAGAAGGCGAACAAGAGAGGTGGAAGGAGAGAATTTCTAGCCTTCAATTCTATGGTAGGGCTTCCTCATATGAGGAGGAGAAGAAGCAGAGGACTTCTCGTGGAATTCTTAAGGCTAGCTAAGGATTTGTTAGCCAATTCGGCAAGAGGTATCATAACTTTCGGTGACGGAGATGCTTGTGTGAAAAGGGGAAATGACTCGAGTTTCAACTCCTCCTTCGACGCTAATGTTGTGCACTACAAAGCCTTGTTAGAGTCTCTTGAATCGAGCTTCCCGACCCATCTAGGAGAGGCCAGGATTGTGATGGAGTTGATGTTTGTTGCACCGTATGTATCTTCTCACGCATGGTTCGAGAAGTGGAACGAAGCAAGAGAAGGTGGAAATCTTCAGCCCTGGTTCGGGCTGGAGGGTAGGAGAGTGAGCAGGGAAATGTTAATGGAGGCCAAAGAAATTGTGGGAGCAGATAGCTCGTATGGAGTGAGAGTAGGAGGAGAGAATGGGAATGAGATGACTTTGGAATGGAATTCAACTCCGTTGGTTAGAGTTGAAACCTGGACAAACAGAAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GRAS

Protein Analysis

381

Amino Acids

43.17

Weight (kDa)

5.48

Isoelectric Point (pI)

52.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRAS PF03514 10 - 377 2.7e-42 GRAS domain family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 299
AccII CGCG 1 cut(s) 548
AciI CCGC 3 cut(s) 107, 228, 291
AcsI RAATTY 5 cut(s) 217, 341, 605, 677, 1096
AcuI CTGAAG 1 cut(s) 944
AfiI CCNNNNNNNGG 2 cut(s) 971, 972
AflII CTTAAG 1 cut(s) 682
AjnI CCWGG 3 cut(s) 866, 965, 1124
AluBI AGCT 6 cut(s) 79, 376, 692, 843, 1041, 1140
AluI AGCT 6 cut(s) 79, 376, 692, 843, 1041, 1140
Alw21I GWGCWC 1 cut(s) 810
Alw26I GTCTC 5 cut(s) 183, 317, 375, 476, 834
Alw44I GTGCAC 1 cut(s) 806
Ama87I CYCGRG 2 cut(s) 299, 772
AoxI GGCC 3 cut(s) 225, 864, 1014
ApaLI GTGCAC 1 cut(s) 806
ApeKI GCWGC 2 cut(s) 281, 455
ApoI RAATTY 5 cut(s) 217, 341, 605, 677, 1096
Asp700I GAANNNNTTC 1 cut(s) 360
AspLEI GCGC 1 cut(s) 144
AsuC2I CCSGG 1 cut(s) 311
AsuHPI GGTGA 4 cut(s) 100, 365, 574, 749
AsuNHI GCTAGC 1 cut(s) 688
AvaI CYCGRG 2 cut(s) 299, 772
BaeGI GKGCMC 1 cut(s) 810
BauI CACGAG 1 cut(s) 671
Bbv12I GWGCWC 1 cut(s) 810
BbvI GCAGC 2 cut(s) 293, 442
BccI CCATC 4 cut(s) 39, 58, 861, 871
BcgI CGANNNNNNTGC 2 cut(s) 1023, 1057
BciT130I CCWGG 3 cut(s) 868, 967, 1126
BclI TGATCA 1 cut(s) 90
BcnI CCSGG 1 cut(s) 311
BcoDI GTCTC 5 cut(s) 183, 317, 375, 476, 834
BfaI CTAG 4 cut(s) 611, 689, 857, 1141
BfoI RGCGCY 1 cut(s) 145
BfrI CTTAAG 1 cut(s) 682
BglI GCCNNNNNGGC 1 cut(s) 713
BisI GCNGC 3 cut(s) 228, 282, 456
BlsI GCNGC 3 cut(s) 229, 283, 457
Bme1390I CCNGG 4 cut(s) 311, 868, 967, 1126
BmeT110I CYCGRG 2 cut(s) 299, 772
BmrFI CCNGG 4 cut(s) 311, 868, 967, 1126
BmsI GCATC 2 cut(s) 89, 736
BmtI GCTAGC 1 cut(s) 692
BplI GAGNNNNNCTC 4 cut(s) 256, 288, 654, 686
BpmI CTGGAG 2 cut(s) 101, 998
Bpu10I CCTNAGC 1 cut(s) 693
BpuEI CTTGAG 2 cut(s) 23, 158
BpuMI CCSGG 1 cut(s) 311
BsaJI CCNNGG 1 cut(s) 965
BsaXI ACNNNNNCTCC 4 cut(s) 872, 902, 1041, 1071
Bsc4I CCNNNNNNNGG 2 cut(s) 971, 972
Bse3DI GCAATG 1 cut(s) 63
BseBI CCWGG 3 cut(s) 868, 967, 1126
BseDI CCNNGG 1 cut(s) 965
BseGI GGATG 2 cut(s) 322, 439
BseLI CCNNNNNNNGG 2 cut(s) 971, 972
BseMI GCAATG 1 cut(s) 63
BseMII CTCAG 1 cut(s) 386
BseRI GAGGAG 7 cut(s) 98, 450, 554, 661, 664, 775, 1077
BseSI GKGCMC 1 cut(s) 810
BseXI GCAGC 2 cut(s) 293, 442
Bsh1236I CGCG 1 cut(s) 548
BshFI GGCC 3 cut(s) 227, 866, 1016
BsiHKAI GWGCWC 1 cut(s) 810
BsiHKCI CYCGRG 2 cut(s) 299, 772
BsiSI CCGG 1 cut(s) 311
BslFI GGGAC 1 cut(s) 293
BslI CCNNNNNNNGG 2 cut(s) 971, 972
BsmAI GTCTC 5 cut(s) 183, 317, 375, 476, 834
BsmBI CGTCTC 1 cut(s) 317
BsmFI GGGAC 1 cut(s) 293
BsmI GAATGC 1 cut(s) 149
BsnI GGCC 3 cut(s) 227, 866, 1016
BsoBI CYCGRG 2 cut(s) 299, 772
Bsp1286I GDGCHC 1 cut(s) 810
Bsp143I GATC 2 cut(s) 90, 543
Bsp68I TCGCGA 1 cut(s) 548
BspACI CCGC 3 cut(s) 107, 228, 291
BspANI GGCC 3 cut(s) 227, 866, 1016
BspCNI CTCAG 1 cut(s) 385
BspFNI CGCG 1 cut(s) 548
BspHI TCATGA 1 cut(s) 93
BspOI GCTAGC 1 cut(s) 692
BspTI CTTAAG 1 cut(s) 682
BsrDI GCAATG 1 cut(s) 63
BssECI CCNNGG 1 cut(s) 965
BssMI GATC 2 cut(s) 90, 543
BssSI CACGAG 1 cut(s) 671
Bst2BI CACGAG 1 cut(s) 671
Bst2UI CCWGG 3 cut(s) 868, 967, 1126
Bst4CI ACNGT 3 cut(s) 328, 468, 900
Bst6I CTCTTC 1 cut(s) 435
BstAFI CTTAAG 1 cut(s) 682
BstC8I GCNNGC 1 cut(s) 690
BstDEI CTNAG 2 cut(s) 372, 693
BstF5I GGATG 2 cut(s) 322, 439
BstFNI CGCG 1 cut(s) 548
BstH2I RGCGCY 1 cut(s) 145
BstHHI GCGC 1 cut(s) 144
BstKTI GATC 2 cut(s) 93, 546
BstMAI GTCTC 5 cut(s) 183, 317, 375, 476, 834
BstMBI GATC 2 cut(s) 90, 543
BstMWI GCNNNNNNNGC 4 cut(s) 76, 278, 290, 713
BstNI CCWGG 3 cut(s) 868, 967, 1126
BstSCI CCNGG 4 cut(s) 309, 866, 965, 1124
BstSLI GKGCMC 1 cut(s) 810
BstUI CGCG 1 cut(s) 548
BstV1I GCAGC 2 cut(s) 293, 442
BsuRI GGCC 3 cut(s) 227, 866, 1016
BtsCI GGATG 2 cut(s) 322, 439
BtsIMutI CAGTG 1 cut(s) 473
BtuMI TCGCGA 1 cut(s) 548
Cac8I GCNNGC 1 cut(s) 690
CciI TCATGA 1 cut(s) 93
CfoI GCGC 1 cut(s) 144
CseI GACGC 1 cut(s) 803
CspCI CAANNNNNGTGG 2 cut(s) 574, 609
CviAII CATG 4 cut(s) 94, 332, 506, 918
DdeI CTNAG 2 cut(s) 372, 693
DpnI GATC 2 cut(s) 92, 545
DpnII GATC 2 cut(s) 90, 543
Eam1104I CTCTTC 1 cut(s) 435
EarI CTCTTC 1 cut(s) 435
Eco32I GATATC 1 cut(s) 349
Eco57I CTGAAG 1 cut(s) 944
Eco88I CYCGRG 2 cut(s) 299, 772
EcoRI GAATTC 2 cut(s) 677, 1096
EcoRII CCWGG 3 cut(s) 866, 965, 1124
EcoRV GATATC 1 cut(s) 349
Esp3I CGTCTC 1 cut(s) 317
FaeI CATG 4 cut(s) 97, 335, 509, 921
FaqI GGGAC 1 cut(s) 293
FatI CATG 4 cut(s) 93, 331, 505, 917
FauNDI CATATG 1 cut(s) 642
FbaI TGATCA 1 cut(s) 90
Fnu4HI GCNGC 3 cut(s) 228, 282, 456
FokI GGATG 2 cut(s) 329, 446
Fsp4HI GCNGC 3 cut(s) 228, 282, 456
FspBI CTAG 4 cut(s) 611, 689, 857, 1141
GlaI GCGC 1 cut(s) 143
GluI GCNGC 3 cut(s) 228, 282, 456
GsuI CTGGAG 2 cut(s) 101, 998
HaeII RGCGCY 1 cut(s) 145
HaeIII GGCC 3 cut(s) 227, 866, 1016
HapII CCGG 1 cut(s) 311
HgaI GACGC 1 cut(s) 803
HhaI GCGC 1 cut(s) 144
Hin1II CATG 4 cut(s) 97, 335, 509, 921
Hin6I GCGC 1 cut(s) 142
HinP1I GCGC 1 cut(s) 142
HinfI GANTC 6 cut(s) 206, 383, 474, 770, 827, 836
HpaII CCGG 1 cut(s) 311
HphI GGTGA 4 cut(s) 100, 365, 574, 749
Hpy166II GTNNAC 3 cut(s) 118, 526, 808
Hpy188III TCNNGA 8 cut(s) 94, 137, 322, 547, 574, 833, 847, 925
Hpy8I GTNNAC 3 cut(s) 118, 526, 808
Hpy99I CGWCG 1 cut(s) 797
HpyAV CCTTC 7 cut(s) 350, 526, 571, 591, 625, 799, 941
HpyCH4III ACNGT 3 cut(s) 328, 468, 900
HpyCH4V TGCA 5 cut(s) 149, 213, 509, 808, 896
HpyF10VI GCNNNNNNNGC 4 cut(s) 76, 278, 290, 713
HpyF3I CTNAG 2 cut(s) 372, 693
Hsp92II CATG 4 cut(s) 97, 335, 509, 921
HspAI GCGC 1 cut(s) 142
Ksp22I TGATCA 1 cut(s) 90
Kzo9I GATC 2 cut(s) 90, 543
LmnI GCTCC 3 cut(s) 76, 463, 1031
Lsp1109I GCAGC 2 cut(s) 293, 442
LweI GCATC 2 cut(s) 89, 736
MaeI CTAG 4 cut(s) 611, 689, 857, 1141
MaeIII GTNAC 2 cut(s) 415, 737
MalI GATC 2 cut(s) 92, 545
MboI GATC 2 cut(s) 90, 543
MboII GAAGA 5 cut(s) 244, 452, 666, 900, 950
MfeI CAATTG 1 cut(s) 365
MhlI GDGCHC 1 cut(s) 810
MlyI GAGTC 3 cut(s) 392, 764, 836
MmeI TCCRAC 1 cut(s) 523
MroXI GAANNNNTTC 1 cut(s) 360
MseI TTAA 3 cut(s) 426, 683, 1007
MspA1I CMGCKG 1 cut(s) 376
MspCI CTTAAG 1 cut(s) 682
MspI CCGG 1 cut(s) 311
MspR9I CCNGG 4 cut(s) 311, 868, 967, 1126
MunI CAATTG 1 cut(s) 365
Mva1269I GAATGC 1 cut(s) 149
MvaI CCWGG 3 cut(s) 868, 967, 1126
MvnI CGCG 1 cut(s) 548
MwoI GCNNNNNNNGC 4 cut(s) 76, 278, 290, 713
NciI CCSGG 1 cut(s) 311
NdeI CATATG 1 cut(s) 642
NdeII GATC 2 cut(s) 90, 543
NheI GCTAGC 1 cut(s) 688
NlaIII CATG 4 cut(s) 97, 335, 509, 921
NmuCI GTSAC 2 cut(s) 415, 737
NruI TCGCGA 1 cut(s) 548
PaeR7I CTCGAG 2 cut(s) 299, 772
PagI TCATGA 1 cut(s) 93
PcsI WCGNNNNNNNCGW 2 cut(s) 845, 921
PctI GAATGC 1 cut(s) 149
PdmI GAANNNNTTC 1 cut(s) 360
PfeI GAWTC 3 cut(s) 206, 474, 836
PfoI TCCNGGA 1 cut(s) 309
PkrI GCNGC 3 cut(s) 229, 283, 457
PleI GAGTC 3 cut(s) 391, 764, 835
PpsI GAGTC 3 cut(s) 391, 764, 835
Psp6I CCWGG 3 cut(s) 866, 965, 1124
PspGI CCWGG 3 cut(s) 866, 965, 1124
PspXI VCTCGAGB 2 cut(s) 299, 772
PvuII CAGCTG 1 cut(s) 376
RruI TCGCGA 1 cut(s) 548
SaqAI TTAA 3 cut(s) 426, 683, 1007
SatI GCNGC 3 cut(s) 228, 282, 456
Sau3AI GATC 2 cut(s) 90, 543
SchI GAGTC 3 cut(s) 392, 764, 836
ScrFI CCNGG 4 cut(s) 311, 868, 967, 1126
SduI GDGCHC 1 cut(s) 810
SfaNI GCATC 2 cut(s) 89, 736
Sfr274I CTCGAG 2 cut(s) 299, 772
SlaI CTCGAG 2 cut(s) 299, 772
SmlI CTYRAG 5 cut(s) 38, 137, 299, 682, 772
SmoI CTYRAG 5 cut(s) 38, 137, 299, 682, 772
SsiI CCGC 3 cut(s) 107, 228, 291
SspMI CTAG 4 cut(s) 611, 689, 857, 1141
StyD4I CCNGG 4 cut(s) 309, 866, 965, 1124
TaaI ACNGT 3 cut(s) 328, 468, 900
TaqI TCGA 7 cut(s) 9, 300, 321, 773, 792, 839, 924
TauI GCSGC 1 cut(s) 230
TfiI GAWTC 3 cut(s) 206, 474, 836
Tru1I TTAA 3 cut(s) 426, 683, 1007
Tru9I TTAA 3 cut(s) 426, 683, 1007
TscAI CASTG 1 cut(s) 473
TseFI GTSAC 2 cut(s) 415, 737
TseI GCWGC 2 cut(s) 281, 455
Tsp45I GTSAC 2 cut(s) 415, 737
TspDTI ATGAA 1 cut(s) 320
TspGWI ACGGA 2 cut(s) 756, 1095
TspRI CASTG 1 cut(s) 473
Vha464I CTTAAG 1 cut(s) 682
VneI GTGCAC 1 cut(s) 806
XapI RAATTY 5 cut(s) 217, 341, 605, 677, 1096
XcmI CCANNNNNNNNNTGG 2 cut(s) 874, 1024
XhoI CTCGAG 2 cut(s) 299, 772
XmnI GAANNNNTTC 1 cut(s) 360
XspI CTAG 4 cut(s) 611, 689, 857, 1141
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.