MD04G1163400.v1.1

Belongs to the GRAS family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0004862
Physical Location & Seq
Forward (+)
25477063 .. 25478646
1584 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1163400.v1.1.491

Sequence Viewer

Length: 1584 bp
ATGATGCAACCTGGGATGTTTCAGACCTTGTGGCCGTACCTGAATGTAACAAATTCACCTTCGGATACAAATGAATACACTGATCAGATGGGGAGCTCTGAATTTTCTTCCATTTTCATGGCTTCGGACGAATTTTCAGAAACTTCTTTTCCATTTTCAACCATGTTTTCTGGTGAATTTGAACAAGTTGCAGATAGTGATCATCTCCTGCAAGTCTATTCGACCGAGGTGGAGGAATTTCCGATGGAGTTGGGAGGATTCGAGCCTAACTATTTAATCGGTGAGATAGAAAATATGTATGCCTCATGTTTGGAGAGCAGTGAGGGTAGTACTCTTCCTTCGCAGCAATTTTCTGTTGAAGGAAATGATGCGTGGAGTCCAAACTCTTTTGTGACATCTGAGACATCCGTCCAACAATCAATAACCCTGCCGATAGACGAGATGGAAATCGACAACAAAGTGAGTATTCACCATCTGCTCAAGGCGTTCGGTGAGGCCATGGAGATGGGGCAGAGGGAGCTGGGGAAGGTGATCATGAGATGCCTTGCGGAGAAAGTAAACCCACTTGGTCAATCCCTTGAGCGCGTTGCGTTCAACTTATGTCAAGGAGTTGTTGATAACCAGCACAGAGACTATTTGAAACAAGAATCCTGCAAGAATTTTGAGGCAGCATTCAACTTGTTCTACCAAATCTTCCCTTTTGGAAGATTTGCTCACTACGCAGCAAACACCGCAATCCTCAAGGCTATCCCAGAAGATGTAGAGACGGTTCATGTAGTGGAATTTGATATCGGTGAAGGGGTTCAATTGTCTCAGCTGATTGAGGCTGTGGCAAAGAGAAACAAAACATTAAAAGTGACAGCAATTAAATGGGATGTGGATGAGAGAGATGAGGTTGCTCCTCCACAGTGGAGATTTGAGGAGACGAAAAGGCAACTCCAGCATCACGCAAGGTCTTTTGGTCTAAACTTGAAGGTGGAGGAGATCACGATCGAGGATTTGGTGAGCGAAATCAAGAAGGTGAACAAAAGAGGTGGAAGAAGAGAATTTATAGCCTTCAACACTATGGTAGGGCTTCCTCACATGAGGAGGAGAAGAAGCAGAGGACTTGTCATGGAATTCCTAAGGCTAGTTAAGAATTTACTAGCCAATTCGGCAAGAGGTATCATAACGTTTGGTGATGGAGATGCTTGCGCGTTAAGGGGAAATGACTCGAGTTTCAGCTCCTTCTTTGACACGAATGTTGTGCATTACAAAGCCTTGTTAGAGTCTTTGGAATCAACCTTCCCGAGCCACCTAGGAGAGGCAAGGATGGTGATGGAGTTGTTGTTTGTGGCACCGTATGTGTCTTCTCAGGCTTGGCTCGATAAGTGGAACGAAGCAAGAGAAGGCAGAAATCTTCAGTCCTGGTTCGGGCTTGAGGGTAGGCGAGTGAGCGGGGAAATGTTGACGGAGGCCAAAGAAATGGTGGGAGCAGATAGCTCGTACGGAGTGAGAATTGGAGGAGAGAATGCGAATGAGATGGCCTTGGAATGGAATGGAATTCCTTTGGTTACAGTGGAAACCTGGACAAACCAAAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GRAS

Protein Analysis

528

Amino Acids

59.59

Weight (kDa)

4.82

Isoelectric Point (pI)

50.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRAS PF03514 157 - 524 7.1e-41 GRAS domain family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1336
AccBSI CCGCTC 1 cut(s) 1437
AccII CGCG 2 cut(s) 585, 1196
AciI CCGC 3 cut(s) 548, 732, 1437
AclI AACGTT 1 cut(s) 1172
AcoI YGGCCR 1 cut(s) 32
AcuI CTGAAG 1 cut(s) 1385
AfaI GTAC 3 cut(s) 38, 331, 1487
AfiI CCNNNNNNNGG 3 cut(s) 1303, 1413, 1533
AgsI TTSAA 9 cut(s) 159, 182, 359, 595, 640, 676, 806, 973, 1060
AjnI CCWGG 3 cut(s) 10, 1406, 1565
AluBI AGCT 6 cut(s) 96, 520, 817, 1224, 1482, 1581
AluI AGCT 6 cut(s) 96, 520, 817, 1224, 1482, 1581
Alw21I GWGCWC 1 cut(s) 98
Alw26I GTCTC 5 cut(s) 395, 624, 758, 816, 917
Ama87I CYCGRG 2 cut(s) 1213, 1288
AoxI GGCC 4 cut(s) 32, 495, 1455, 1524
ApeKI GCWGC 3 cut(s) 343, 668, 722
Asp700I GAANNNNTTC 1 cut(s) 801
AspA2I CCTAGG 1 cut(s) 1297
AspLEI GCGC 2 cut(s) 585, 1196
AvaI CYCGRG 2 cut(s) 1213, 1288
AvrII CCTAGG 1 cut(s) 1297
AxyI CCTNAGG 1 cut(s) 1124
BanI GGYRCC 1 cut(s) 1336
BanII GRGCYC 1 cut(s) 98
BarI GAAGNNNNNNTAC 2 cut(s) 322, 354
BbsI GAAGAC 1 cut(s) 1341
Bbv12I GWGCWC 1 cut(s) 98
BbvI GCAGC 3 cut(s) 355, 680, 734
BccI CCATC 9 cut(s) 82, 238, 436, 480, 499, 1175, 1306, 1312, 1516
BceAI ACGGC 1 cut(s) 19
BcgI CGANNNNNNTGC 4 cut(s) 1228, 1262, 1464, 1498
BciT130I CCWGG 3 cut(s) 12, 1408, 1567
BciVI GTATCC 1 cut(s) 58
BclI TGATCA 3 cut(s) 82, 199, 531
BcoDI GTCTC 5 cut(s) 395, 624, 758, 816, 917
BfaI CTAG 4 cut(s) 1130, 1145, 1298, 1582
BfuI GTATCC 1 cut(s) 58
BglI GCCNNNNNGGC 1 cut(s) 1154
BisI GCNGC 3 cut(s) 344, 669, 723
BlnI CCTAGG 1 cut(s) 1297
BlsI GCNGC 3 cut(s) 345, 670, 724
BmcAI AGTACT 1 cut(s) 331
Bme1390I CCNGG 3 cut(s) 12, 1408, 1567
BmeT110I CYCGRG 2 cut(s) 1213, 1288
BmiI GGNNCC 1 cut(s) 1338
BmrFI CCNGG 3 cut(s) 12, 1408, 1567
BmsI GCATC 4 cut(s) 358, 530, 952, 1177
BoxI GACNNNNGTC 1 cut(s) 407
BpiI GAAGAC 1 cut(s) 1341
BpmI CTGGAG 1 cut(s) 923
BpuEI CTTGAG 4 cut(s) 464, 599, 725, 1439
BsaBI GATNNNNATC 3 cut(s) 198, 446, 989
BsaJI CCNNGG 5 cut(s) 11, 225, 498, 1297, 1527
BsaXI ACNNNNNCTCC 2 cut(s) 1313, 1343
Bsc4I CCNNNNNNNGG 3 cut(s) 1303, 1413, 1533
Bse21I CCTNAGG 1 cut(s) 1124
Bse8I GATNNNNATC 3 cut(s) 198, 446, 989
BseBI CCWGG 3 cut(s) 12, 1408, 1567
BseDI CCNNGG 5 cut(s) 11, 225, 498, 1297, 1527
BseGI GGATG 5 cut(s) 21, 404, 880, 886, 1317
BseJI GATNNNNATC 3 cut(s) 198, 446, 989
BseLI CCNNNNNNNGG 3 cut(s) 1303, 1413, 1533
BseMII CTCAG 3 cut(s) 390, 827, 1367
BseRI GAGGAG 6 cut(s) 891, 935, 995, 1102, 1105, 1518
BseXI GCAGC 3 cut(s) 355, 680, 734
BseYI CCCAGC 1 cut(s) 520
Bsh1236I CGCG 2 cut(s) 585, 1196
Bsh1285I CGRYCG 2 cut(s) 225, 993
BshFI GGCC 4 cut(s) 34, 497, 1457, 1526
BshNI GGYRCC 1 cut(s) 1336
BsiEI CGRYCG 2 cut(s) 225, 993
BsiHKAI GWGCWC 1 cut(s) 98
BsiHKCI CYCGRG 2 cut(s) 1213, 1288
BsiWI CGTACG 1 cut(s) 1485
BslI CCNNNNNNNGG 3 cut(s) 1303, 1413, 1533
BsmAI GTCTC 5 cut(s) 395, 624, 758, 816, 917
BsmBI CGTCTC 2 cut(s) 758, 917
BsmI GAATGC 2 cut(s) 671, 1516
BsnI GGCC 4 cut(s) 34, 497, 1457, 1526
BsoBI CYCGRG 2 cut(s) 1213, 1288
Bsp1286I GDGCHC 1 cut(s) 98
Bsp143I GATC 5 cut(s) 82, 199, 531, 984, 990
Bsp19I CCATGG 1 cut(s) 498
BspACI CCGC 3 cut(s) 548, 732, 1437
BspANI GGCC 4 cut(s) 34, 497, 1457, 1526
BspCNI CTCAG 3 cut(s) 391, 826, 1366
BspFNI CGCG 2 cut(s) 585, 1196
BspHI TCATGA 1 cut(s) 534
BspLI GGNNCC 1 cut(s) 1338
BspT107I GGYRCC 1 cut(s) 1336
BsrBI CCGCTC 1 cut(s) 1437
BssECI CCNNGG 5 cut(s) 11, 225, 498, 1297, 1527
BssMI GATC 5 cut(s) 82, 199, 531, 984, 990
BssT1I CCWWGG 3 cut(s) 498, 1297, 1527
Bst2UI CCWGG 3 cut(s) 12, 1408, 1567
Bst4CI ACNGT 4 cut(s) 769, 909, 1341, 1558
Bst6I CTCTTC 2 cut(s) 339, 1036
BstC8I GCNNGC 1 cut(s) 1192
BstDEI CTNAG 4 cut(s) 399, 813, 1124, 1353
BstDSI CCRYGG 1 cut(s) 498
BstENI CCTNNNNNAGG 1 cut(s) 1301
BstF5I GGATG 5 cut(s) 21, 404, 880, 886, 1317
BstFNI CGCG 2 cut(s) 585, 1196
BstHHI GCGC 2 cut(s) 585, 1196
BstKTI GATC 5 cut(s) 85, 202, 534, 987, 993
BstMAI GTCTC 5 cut(s) 395, 624, 758, 816, 917
BstMBI GATC 5 cut(s) 82, 199, 531, 984, 990
BstMCI CGRYCG 2 cut(s) 225, 993
BstMWI GCNNNNNNNGC 5 cut(s) 517, 719, 731, 940, 1154
BstNI CCWGG 3 cut(s) 12, 1408, 1567
BstPAI GACNNNNGTC 1 cut(s) 407
BstSCI CCNGG 3 cut(s) 10, 1406, 1565
BstUI CGCG 2 cut(s) 585, 1196
BstV1I GCAGC 3 cut(s) 355, 680, 734
BstV2I GAAGAC 1 cut(s) 1341
BstXI CCANNNNNNTGG 3 cut(s) 118, 505, 1465
Bsu36I CCTNAGG 1 cut(s) 1124
BsuI GTATCC 1 cut(s) 58
BsuRI GGCC 4 cut(s) 34, 497, 1457, 1526
BtgI CCRYGG 1 cut(s) 498
BtsCI GGATG 5 cut(s) 21, 404, 880, 886, 1317
BtsI GCAGTG 1 cut(s) 325
BtsIMutI CAGTG 4 cut(s) 78, 325, 914, 1563
Cac8I GCNNGC 1 cut(s) 1192
CciI TCATGA 1 cut(s) 534
CfoI GCGC 2 cut(s) 585, 1196
Csp6I GTAC 3 cut(s) 37, 330, 1486
CspCI CAANNNNNGTGG 2 cut(s) 1015, 1050
CviAII CATG 8 cut(s) 118, 163, 306, 499, 535, 773, 1084, 1114
CviQI GTAC 3 cut(s) 37, 330, 1486
DdeI CTNAG 4 cut(s) 399, 813, 1124, 1353
DpnI GATC 5 cut(s) 84, 201, 533, 986, 992
DpnII GATC 5 cut(s) 82, 199, 531, 984, 990
EaeI YGGCCR 1 cut(s) 32
Eam1104I CTCTTC 2 cut(s) 339, 1036
EarI CTCTTC 2 cut(s) 339, 1036
Ecl136II GAGCTC 1 cut(s) 96
Eco130I CCWWGG 3 cut(s) 498, 1297, 1527
Eco24I GRGCYC 1 cut(s) 98
Eco32I GATATC 1 cut(s) 790
Eco53kI GAGCTC 1 cut(s) 96
Eco57I CTGAAG 1 cut(s) 1385
Eco81I CCTNAGG 1 cut(s) 1124
Eco88I CYCGRG 2 cut(s) 1213, 1288
EcoICRI GAGCTC 1 cut(s) 96
EcoNI CCTNNNNNAGG 1 cut(s) 1301
EcoRI GAATTC 2 cut(s) 1118, 1542
EcoRII CCWGG 3 cut(s) 10, 1406, 1565
EcoRV GATATC 1 cut(s) 790
EcoT14I CCWWGG 3 cut(s) 498, 1297, 1527
EcoT38I GRGCYC 1 cut(s) 98
ErhI CCWWGG 3 cut(s) 498, 1297, 1527
Esp3I CGTCTC 2 cut(s) 758, 917
FaeI CATG 8 cut(s) 121, 166, 309, 502, 538, 776, 1087, 1117
FatI CATG 8 cut(s) 117, 162, 305, 498, 534, 772, 1083, 1113
FauI CCCGC 1 cut(s) 1430
FbaI TGATCA 3 cut(s) 82, 199, 531
Fnu4HI GCNGC 3 cut(s) 344, 669, 723
FokI GGATG 5 cut(s) 28, 391, 887, 893, 1324
FriOI GRGCYC 1 cut(s) 98
Fsp4HI GCNGC 3 cut(s) 344, 669, 723
FspBI CTAG 4 cut(s) 1130, 1145, 1298, 1582
GlaI GCGC 2 cut(s) 584, 1195
GluI GCNGC 3 cut(s) 344, 669, 723
GsaI CCCAGC 1 cut(s) 524
GsuI CTGGAG 1 cut(s) 923
HaeIII GGCC 4 cut(s) 34, 497, 1457, 1526
HhaI GCGC 2 cut(s) 585, 1196
Hin1II CATG 8 cut(s) 121, 166, 309, 502, 538, 776, 1087, 1117
Hin6I GCGC 2 cut(s) 583, 1194
HinP1I GCGC 2 cut(s) 583, 1194
HincII GTYRAC 1 cut(s) 1449
HindII GTYRAC 1 cut(s) 1449
HinfI GANTC 6 cut(s) 258, 376, 647, 1211, 1268, 1277
Hpy166II GTNNAC 3 cut(s) 559, 1024, 1449
Hpy188I TCNGA 8 cut(s) 24, 64, 87, 100, 127, 139, 243, 400
Hpy188III TCNNGA 4 cut(s) 535, 988, 1015, 1288
Hpy8I GTNNAC 3 cut(s) 559, 1024, 1449
HpyCH4III ACNGT 4 cut(s) 769, 909, 1341, 1558
HpyCH4IV ACGT 1 cut(s) 1172
HpyCH4V TGCA 5 cut(s) 7, 191, 211, 654, 1249
HpyF10VI GCNNNNNNNGC 5 cut(s) 517, 719, 731, 940, 1154
HpyF3I CTNAG 4 cut(s) 399, 813, 1124, 1353
HpySE526I ACGT 1 cut(s) 1172
Hsp92II CATG 8 cut(s) 121, 166, 309, 502, 538, 776, 1087, 1117
HspAI GCGC 2 cut(s) 583, 1194
Ksp22I TGATCA 3 cut(s) 82, 199, 531
Kzo9I GATC 5 cut(s) 82, 199, 531, 984, 990
LmnI GCTCC 5 cut(s) 93, 517, 904, 1229, 1472
Lsp1109I GCAGC 3 cut(s) 355, 680, 734
LweI GCATC 4 cut(s) 358, 530, 952, 1177
MaeI CTAG 4 cut(s) 1130, 1145, 1298, 1582
MaeII ACGT 1 cut(s) 1172
MaeIII GTNAC 4 cut(s) 46, 391, 856, 1552
MalI GATC 5 cut(s) 84, 201, 533, 986, 992
MbiI CCGCTC 1 cut(s) 1437
MboI GATC 5 cut(s) 82, 199, 531, 984, 990
MfeI CAATTG 1 cut(s) 806
MhlI GDGCHC 1 cut(s) 98
MlyI GAGTC 3 cut(s) 385, 1205, 1277
MmeI TCCRAC 1 cut(s) 436
MroXI GAANNNNTTC 1 cut(s) 801
MseI TTAA 5 cut(s) 275, 851, 867, 1134, 1199
MslI CAYNNNNRTG 2 cut(s) 116, 503
MspA1I CMGCKG 1 cut(s) 817
MspR9I CCNGG 3 cut(s) 12, 1408, 1567
MunI CAATTG 1 cut(s) 806
Mva1269I GAATGC 2 cut(s) 671, 1516
MvaI CCWGG 3 cut(s) 12, 1408, 1567
MvnI CGCG 2 cut(s) 585, 1196
MwoI GCNNNNNNNGC 5 cut(s) 517, 719, 731, 940, 1154
NcoI CCATGG 1 cut(s) 498
NdeII GATC 5 cut(s) 82, 199, 531, 984, 990
NlaIII CATG 8 cut(s) 121, 166, 309, 502, 538, 776, 1087, 1117
NlaIV GGNNCC 1 cut(s) 1338
NmuCI GTSAC 2 cut(s) 391, 856
PaeR7I CTCGAG 1 cut(s) 1213
PagI TCATGA 1 cut(s) 534
PctI GAATGC 2 cut(s) 671, 1516
PdmI GAANNNNTTC 1 cut(s) 801
PfeI GAWTC 3 cut(s) 258, 647, 1277
Pfl23II CGTACG 1 cut(s) 1485
PkrI GCNGC 3 cut(s) 345, 670, 724
Ple19I CGATCG 1 cut(s) 993
PleI GAGTC 3 cut(s) 384, 1205, 1276
PpsI GAGTC 3 cut(s) 384, 1205, 1276
PshAI GACNNNNGTC 1 cut(s) 407
Psp124BI GAGCTC 1 cut(s) 98
Psp1406I AACGTT 1 cut(s) 1172
Psp6I CCWGG 3 cut(s) 10, 1406, 1565
PspFI CCCAGC 1 cut(s) 520
PspGI CCWGG 3 cut(s) 10, 1406, 1565
PspLI CGTACG 1 cut(s) 1485
PspN4I GGNNCC 1 cut(s) 1338
PspXI VCTCGAGB 1 cut(s) 1213
PsrI GAACNNNNNNTAC 2 cut(s) 753, 785
PvuI CGATCG 1 cut(s) 993
PvuII CAGCTG 1 cut(s) 817
RsaI GTAC 3 cut(s) 38, 331, 1487
RsaNI GTAC 3 cut(s) 37, 330, 1486
RseI CAYNNNNRTG 2 cut(s) 116, 503
SacI GAGCTC 1 cut(s) 98
SaqAI TTAA 5 cut(s) 275, 851, 867, 1134, 1199
SatI GCNGC 3 cut(s) 344, 669, 723
Sau3AI GATC 5 cut(s) 82, 199, 531, 984, 990
ScaI AGTACT 1 cut(s) 331
SchI GAGTC 3 cut(s) 385, 1205, 1277
ScrFI CCNGG 3 cut(s) 12, 1408, 1567
SduI GDGCHC 1 cut(s) 98
SfaNI GCATC 4 cut(s) 358, 530, 952, 1177
Sfr274I CTCGAG 1 cut(s) 1213
SlaI CTCGAG 1 cut(s) 1213
SmiMI CAYNNNNRTG 2 cut(s) 116, 503
SmlI CTYRAG 5 cut(s) 479, 578, 740, 1213, 1418
SmoI CTYRAG 5 cut(s) 479, 578, 740, 1213, 1418
SsiI CCGC 3 cut(s) 548, 732, 1437
SspMI CTAG 4 cut(s) 1130, 1145, 1298, 1582
SstI GAGCTC 1 cut(s) 98
StyD4I CCNGG 3 cut(s) 10, 1406, 1565
StyI CCWWGG 3 cut(s) 498, 1297, 1527
TaaI ACNGT 4 cut(s) 769, 909, 1341, 1558
TaiI ACGT 1 cut(s) 1175
TaqI TCGA 6 cut(s) 221, 261, 450, 993, 1214, 1365
TaqII GACCGA 1 cut(s) 239
TatI WGTACW 1 cut(s) 329
TfiI GAWTC 3 cut(s) 258, 647, 1277
Tru1I TTAA 5 cut(s) 275, 851, 867, 1134, 1199
Tru9I TTAA 5 cut(s) 275, 851, 867, 1134, 1199
TscAI CASTG 4 cut(s) 85, 325, 914, 1563
TseFI GTSAC 2 cut(s) 391, 856
TseI GCWGC 3 cut(s) 343, 668, 722
Tsp45I GTSAC 2 cut(s) 391, 856
TspDTI ATGAA 3 cut(s) 87, 106, 761
TspGWI ACGGA 3 cut(s) 397, 1466, 1503
TspRI CASTG 4 cut(s) 85, 325, 914, 1563
XagI CCTNNNNNAGG 1 cut(s) 1301
XcmI CCANNNNNNNNNTGG 1 cut(s) 1465
XhoI CTCGAG 1 cut(s) 1213
XmaJI CCTAGG 1 cut(s) 1297
XmnI GAANNNNTTC 1 cut(s) 801
XspI CTAG 4 cut(s) 1130, 1145, 1298, 1582
ZrmI AGTACT 1 cut(s) 331
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.