MD12G1176900.v1.1

Belongs to the GRAS family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0004862
Physical Location & Seq
Forward (+)
25734748 .. 25736358
1611 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1176900.v1.1.491

Sequence Viewer

Length: 1611 bp
ATGATGCAATCTGAGATGAACTTTCAGACCTTGTGGCCATACCTGAATGTAACAAATTCACCATTGGATACAAATGAATACACTGAGCAGATGGGGAGCTTTGAATTTTCTTCAATTTTCATGGCATCGGACAAGTTTTCAGAAACTACTTCTTTTCCATTTTCAACCATGTTTTCTGGTGAATTTGAACAACTCGCAGATTGTGATAATCTCCTGCAAGTCACATCGATGGCGGAAGAGGAATTTCCCATGGAGTTGGGAGGATTCGAGACGAATTATTTGATCCGTGAGATAGAAAATATGTATGCCTCATGTTTGGAAGGCAGTGAGGGAAGCAATACTCTTCCTTCGCAGCAATTTTCTGTTGAAGGAAATGATGTGTGGAGTCCAAACTCGTTTGTGACATCTGAGGCATCCTCCATGGATGGGACATCCATCCAACAATCACTAATCCTGCCAAGAGACGAGATGGAAATCGACAACGAAGTGAGTATTCTCCATCTGGTCAAGGCGTTTGGCGAGGCCATGGAGATGGGGCAGAGGGAGTTGGAGGTGGTGATCTTGAGATGCCTTGTGGAGAAAGTAAACCCACTTGGTCAATCTCTTGAGCGCCTTGCATTCAACTTGTGCCAAGAAGTTGTTGATCATCAGCAGGGAGACCATTTGAAGCAAGAATGTAGCAAGAATTTCGAGGTTGCATTCAACTTGTTCTACCAAATATTCCCTTTTGGGAGATTTGCTCACTATGCAGCAAACACCGCAATCCTTGAAGCTGTCCCCGAGGATGTGGAGATGGTTCATGTAGTTGAATTCGATATGGGGGAAGGGGTTCAATTGTCCCAGTTGATTGAGGCTTTGGCAAAGAGAAACAAAGCACTTAGAGTGACAGCAATTAAATGGGATGTGGAAGAGAGTGATGAGGTTGCTCCTCCACAGTGGAGATTTGAGGAGACGAAAAGGCAACTCCAGCATCACGCAAGGTCATTCGGGCTAAACTTGAAGGTGGAGGAGATCCCGATTGAGGATTTGGTGAGCAAAATCAAAAAGGTGAACAAGAGAGGGGGAATGAGAGAATTTCTAGCCTTCAACTCTATGGTGGGGCTTCCTCATATGAGGAGGAGAAGAAGCAGAGGACTTCTCATGGAATTCCTAAGGCTAGCTAAGGATTTGTTAGCCAATTCGGCAAGAGGTATCATAACTTTTGGTGACGGAGATGCATGCGCGAAAAGGGAAAATGACTCGAGTTTCAGCTCCTTGTTTGACTCGAATGTTGTACACTACAAAGCCTTGTTAGAGTCTTTGGAATCGAGCTTCCCGATCCATCTAGGAGAGGCAAGGATGGTGATGGAGTTGATGTTTGTGGCGCCATATGTGTCTTCTCAAGCTTGGTTCGAGAAGTGGAACGAATCGAGAGAAGGTGGAAATCTTCAGCCCTGGTTCGGGCTGGAGGGTAGGAGAGTGAGCAGGGAAATGTTAATGGAGGCCAAAGAAATTGTGGGAGCAGATAGCTCGTATGGAGTGAGAGTTGGAGGAGAGGACGGGAATGAGATGGCTTTGGAATGGAATTCAATTCCTTTGATTAGAGTTGAAACCTGGACAAACCGAAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GRAS

Protein Analysis

537

Amino Acids

60.96

Weight (kDa)

4.7

Isoelectric Point (pI)

54.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRAS PF03514 170 - 533 5.4e-39 GRAS domain family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1363
AccII CGCG 1 cut(s) 1223
AciI CCGC 2 cut(s) 233, 759
AclWI GGATC 3 cut(s) 277, 1006, 1312
AcoI YGGCCR 1 cut(s) 35
AcsI RAATTY 9 cut(s) 55, 104, 182, 242, 685, 809, 1073, 1145, 1564
AcuI CTGAAG 1 cut(s) 1412
AcyI GRCGYC 1 cut(s) 1364
AfaI GTAC 1 cut(s) 1275
AfiI CCNNNNNNNGG 4 cut(s) 426, 1021, 1439, 1440
AjnI CCWGG 2 cut(s) 1433, 1592
AluBI AGCT 8 cut(s) 99, 773, 1160, 1251, 1311, 1385, 1509, 1608
AluI AGCT 8 cut(s) 99, 773, 1160, 1251, 1311, 1385, 1509, 1608
Alw26I GTCTC 4 cut(s) 263, 456, 651, 944
AlwI GGATC 3 cut(s) 277, 1006, 1312
Ama87I CYCGRG 2 cut(s) 779, 1240
AoxI GGCC 3 cut(s) 35, 522, 1482
ApeKI GCWGC 2 cut(s) 352, 749
ApoI RAATTY 9 cut(s) 55, 104, 182, 242, 685, 809, 1073, 1145, 1564
ArsI GACNNNNNNTTYG 2 cut(s) 262, 294
Asp700I GAANNNNTTC 1 cut(s) 828
AspLEI GCGC 3 cut(s) 612, 1223, 1366
AsuHPI GGTGA 7 cut(s) 51, 191, 568, 1042, 1060, 1217, 1354
AsuNHI GCTAGC 1 cut(s) 1156
AvaI CYCGRG 2 cut(s) 779, 1240
AxyI CCTNAGG 1 cut(s) 1151
BalI TGGCCA 1 cut(s) 37
BanI GGYRCC 1 cut(s) 1363
BbsI GAAGAC 1 cut(s) 1368
BbvI GCAGC 2 cut(s) 364, 761
BcgI CGANNNNNNTGC 4 cut(s) 670, 704, 1491, 1525
BciT130I CCWGG 2 cut(s) 1435, 1594
BciVI GTATCC 1 cut(s) 61
BclI TGATCA 1 cut(s) 643
BcoDI GTCTC 4 cut(s) 263, 456, 651, 944
BfaI CTAG 4 cut(s) 1079, 1157, 1325, 1609
BfoI RGCGCY 2 cut(s) 613, 1367
BfuI GTATCC 1 cut(s) 61
BglI GCCNNNNNGGC 1 cut(s) 1181
BisI GCNGC 2 cut(s) 353, 750
BlsI GCNGC 2 cut(s) 354, 751
Bme1390I CCNGG 2 cut(s) 1435, 1594
BmeT110I CYCGRG 2 cut(s) 779, 1240
BmiI GGNNCC 1 cut(s) 1365
BmrFI CCNGG 2 cut(s) 1435, 1594
BmrI ACTGGG 1 cut(s) 835
BmsI GCATC 5 cut(s) 134, 422, 557, 979, 1204
BmtI GCTAGC 1 cut(s) 1160
BmuI ACTGGG 1 cut(s) 835
BpiI GAAGAC 1 cut(s) 1368
BplI GAGNNNNNCTC 6 cut(s) 401, 433, 724, 756, 1122, 1154
BpmI CTGGAG 2 cut(s) 950, 1466
Bpu10I CCTNAGC 1 cut(s) 1161
BpuEI CTTGAG 3 cut(s) 583, 626, 1365
Bsa29I ATCGAT 1 cut(s) 227
BsaBI GATNNNNATC 1 cut(s) 473
BsaHI GRCGYC 1 cut(s) 1364
BsaI GGTCTC 1 cut(s) 651
BsaJI CCNNGG 5 cut(s) 249, 420, 525, 780, 1433
BsaXI ACNNNNNCTCC 4 cut(s) 1340, 1370, 1509, 1539
Bsc4I CCNNNNNNNGG 4 cut(s) 426, 1021, 1439, 1440
Bse1I ACTGG 1 cut(s) 841
Bse21I CCTNAGG 1 cut(s) 1151
Bse8I GATNNNNATC 1 cut(s) 473
BseBI CCWGG 2 cut(s) 1435, 1594
BseCI ATCGAT 1 cut(s) 227
BseDI CCNNGG 5 cut(s) 249, 420, 525, 780, 1433
BseGI GGATG 7 cut(s) 413, 430, 431, 435, 790, 907, 1344
BseJI GATNNNNATC 1 cut(s) 473
BseLI CCNNNNNNNGG 4 cut(s) 426, 1021, 1439, 1440
BseMII CTCAG 2 cut(s) 75, 399
BseNI ACTGG 1 cut(s) 841
BseRI GAGGAG 6 cut(s) 918, 962, 1022, 1129, 1132, 1545
BseXI GCAGC 2 cut(s) 364, 761
Bsh1236I CGCG 1 cut(s) 1223
BshFI GGCC 3 cut(s) 37, 524, 1484
BshNI GGYRCC 1 cut(s) 1363
BshVI ATCGAT 1 cut(s) 227
BsiHKCI CYCGRG 2 cut(s) 779, 1240
BslFI GGGAC 3 cut(s) 442, 761, 823
BslI CCNNNNNNNGG 4 cut(s) 426, 1021, 1439, 1440
BsmAI GTCTC 4 cut(s) 263, 456, 651, 944
BsmBI CGTCTC 3 cut(s) 263, 456, 944
BsmFI GGGAC 3 cut(s) 442, 761, 823
BsmI GAATGC 2 cut(s) 617, 698
BsnI GGCC 3 cut(s) 37, 524, 1484
Bso31I GGTCTC 1 cut(s) 651
BsoBI CYCGRG 2 cut(s) 779, 1240
Bsp1407I TGTACA 1 cut(s) 1273
Bsp143I GATC 5 cut(s) 282, 558, 643, 1011, 1317
Bsp19I CCATGG 3 cut(s) 249, 420, 525
BspACI CCGC 2 cut(s) 233, 759
BspANI GGCC 3 cut(s) 37, 524, 1484
BspCNI CTCAG 3 cut(s) 4, 76, 400
BspDI ATCGAT 1 cut(s) 227
BspFNI CGCG 1 cut(s) 1223
BspLI GGNNCC 1 cut(s) 1365
BspOI GCTAGC 1 cut(s) 1160
BspPI GGATC 3 cut(s) 277, 1006, 1312
BspT107I GGYRCC 1 cut(s) 1363
BspTNI GGTCTC 1 cut(s) 651
BsrGI TGTACA 1 cut(s) 1273
BsrI ACTGG 1 cut(s) 841
BssECI CCNNGG 5 cut(s) 249, 420, 525, 780, 1433
BssMI GATC 5 cut(s) 282, 558, 643, 1011, 1317
BssNI GRCGYC 1 cut(s) 1364
BssT1I CCWWGG 3 cut(s) 249, 420, 525
Bst2UI CCWGG 2 cut(s) 1435, 1594
Bst4CI ACNGT 1 cut(s) 936
Bst6I CTCTTC 3 cut(s) 231, 348, 903
BstACI GRCGYC 1 cut(s) 1364
BstAUI TGTACA 1 cut(s) 1273
BstC8I GCNNGC 2 cut(s) 1158, 1219
BstDEI CTNAG 6 cut(s) 12, 84, 408, 878, 1151, 1161
BstDSI CCRYGG 3 cut(s) 249, 420, 525
BstF5I GGATG 7 cut(s) 413, 430, 431, 435, 790, 907, 1344
BstFNI CGCG 1 cut(s) 1223
BstH2I RGCGCY 2 cut(s) 613, 1367
BstHHI GCGC 3 cut(s) 612, 1223, 1366
BstKTI GATC 5 cut(s) 285, 561, 646, 1014, 1320
BstMAI GTCTC 4 cut(s) 263, 456, 651, 944
BstMBI GATC 5 cut(s) 282, 558, 643, 1011, 1317
BstMWI GCNNNNNNNGC 4 cut(s) 746, 758, 967, 1181
BstNI CCWGG 2 cut(s) 1435, 1594
BstNSI RCATGY 1 cut(s) 1221
BstSCI CCNGG 2 cut(s) 1433, 1592
BstUI CGCG 1 cut(s) 1223
BstV1I GCAGC 2 cut(s) 364, 761
BstV2I GAAGAC 1 cut(s) 1368
BstX2I RGATCY 1 cut(s) 1011
BstXI CCANNNNNNTGG 2 cut(s) 256, 532
BstYI RGATCY 1 cut(s) 1011
Bsu15I ATCGAT 1 cut(s) 227
Bsu36I CCTNAGG 1 cut(s) 1151
BsuI GTATCC 1 cut(s) 61
BsuRI GGCC 3 cut(s) 37, 524, 1484
BsuTUI ATCGAT 1 cut(s) 227
BtgI CCRYGG 3 cut(s) 249, 420, 525
BtsCI GGATG 7 cut(s) 413, 430, 431, 435, 790, 907, 1344
BtsI GCAGTG 1 cut(s) 331
BtsIMutI CAGTG 3 cut(s) 81, 331, 941
Cac8I GCNNGC 2 cut(s) 1158, 1219
CfoI GCGC 3 cut(s) 612, 1223, 1366
ClaI ATCGAT 1 cut(s) 227
Csp6I GTAC 1 cut(s) 1274
CviAII CATG 9 cut(s) 121, 169, 250, 312, 421, 526, 800, 1141, 1218
CviQI GTAC 1 cut(s) 1274
DdeI CTNAG 6 cut(s) 12, 84, 408, 878, 1151, 1161
DinI GGCGCC 1 cut(s) 1365
DpnI GATC 5 cut(s) 284, 560, 645, 1013, 1319
DpnII GATC 5 cut(s) 282, 558, 643, 1011, 1317
EaeI YGGCCR 1 cut(s) 35
Eam1104I CTCTTC 3 cut(s) 231, 348, 903
EarI CTCTTC 3 cut(s) 231, 348, 903
EciI GGCGGA 1 cut(s) 248
Eco130I CCWWGG 3 cut(s) 249, 420, 525
Eco31I GGTCTC 1 cut(s) 651
Eco57I CTGAAG 1 cut(s) 1412
Eco81I CCTNAGG 1 cut(s) 1151
Eco88I CYCGRG 2 cut(s) 779, 1240
EcoRI GAATTC 3 cut(s) 809, 1145, 1564
EcoRII CCWGG 2 cut(s) 1433, 1592
EcoT14I CCWWGG 3 cut(s) 249, 420, 525
EcoT22I ATGCAT 1 cut(s) 1219
EgeI GGCGCC 1 cut(s) 1365
EheI GGCGCC 1 cut(s) 1365
ErhI CCWWGG 3 cut(s) 249, 420, 525
Esp3I CGTCTC 3 cut(s) 263, 456, 944
FaeI CATG 9 cut(s) 124, 172, 253, 315, 424, 529, 803, 1144, 1221
FaqI GGGAC 3 cut(s) 442, 761, 823
FatI CATG 9 cut(s) 120, 168, 249, 311, 420, 525, 799, 1140, 1217
FauNDI CATATG 2 cut(s) 1110, 1369
FbaI TGATCA 1 cut(s) 643
Fnu4HI GCNGC 2 cut(s) 353, 750
FokI GGATG 7 cut(s) 400, 418, 422, 437, 797, 914, 1351
Fsp4HI GCNGC 2 cut(s) 353, 750
FspBI CTAG 4 cut(s) 1079, 1157, 1325, 1609
GlaI GCGC 3 cut(s) 611, 1222, 1365
GluI GCNGC 2 cut(s) 353, 750
GsuI CTGGAG 2 cut(s) 950, 1466
HaeII RGCGCY 2 cut(s) 613, 1367
HaeIII GGCC 3 cut(s) 37, 524, 1484
HhaI GCGC 3 cut(s) 612, 1223, 1366
Hin1I GRCGYC 1 cut(s) 1364
Hin1II CATG 9 cut(s) 124, 172, 253, 315, 424, 529, 803, 1144, 1221
Hin6I GCGC 3 cut(s) 610, 1221, 1364
HinP1I GCGC 3 cut(s) 610, 1221, 1364
HindIII AAGCTT 1 cut(s) 1383
HinfI GANTC 7 cut(s) 264, 385, 1238, 1262, 1295, 1304, 1406
HphI GGTGA 7 cut(s) 51, 191, 568, 1042, 1060, 1217, 1354
Hpy166II GTNNAC 3 cut(s) 586, 1051, 1276
Hpy188I TCNGA 5 cut(s) 13, 27, 130, 142, 409
Hpy188III TCNNGA 7 cut(s) 268, 562, 605, 1015, 1315, 1393, 1410
Hpy8I GTNNAC 3 cut(s) 586, 1051, 1276
HpyAV CCTTC 7 cut(s) 314, 357, 362, 818, 994, 1093, 1409
HpyCH4III ACNGT 1 cut(s) 936
HpyCH4V TGCA 6 cut(s) 7, 217, 617, 698, 749, 1217
HpyF10VI GCNNNNNNNGC 4 cut(s) 746, 758, 967, 1181
HpyF3I CTNAG 6 cut(s) 12, 84, 408, 878, 1151, 1161
Hsp92I GRCGYC 1 cut(s) 1364
Hsp92II CATG 9 cut(s) 124, 172, 253, 315, 424, 529, 803, 1144, 1221
HspAI GCGC 3 cut(s) 610, 1221, 1364
KasI GGCGCC 1 cut(s) 1363
Ksp22I TGATCA 1 cut(s) 643
Kzo9I GATC 5 cut(s) 282, 558, 643, 1011, 1317
LmnI GCTCC 4 cut(s) 96, 931, 1256, 1499
Lsp1109I GCAGC 2 cut(s) 364, 761
LweI GCATC 5 cut(s) 134, 422, 557, 979, 1204
MaeI CTAG 4 cut(s) 1079, 1157, 1325, 1609
MaeIII GTNAC 5 cut(s) 49, 220, 400, 883, 1205
MalI GATC 5 cut(s) 284, 560, 645, 1013, 1319
MboI GATC 5 cut(s) 282, 558, 643, 1011, 1317
MboII GAAGA 7 cut(s) 102, 248, 335, 920, 1134, 1368, 1418
MfeI CAATTG 1 cut(s) 833
MflI RGATCY 1 cut(s) 1011
MlsI TGGCCA 1 cut(s) 37
MluNI TGGCCA 1 cut(s) 37
Mly113I GGCGCC 1 cut(s) 1364
MlyI GAGTC 4 cut(s) 394, 1232, 1256, 1304
MmeI TCCRAC 3 cut(s) 463, 528, 1507
Mox20I TGGCCA 1 cut(s) 37
Mph1103I ATGCAT 1 cut(s) 1219
MroXI GAANNNNTTC 1 cut(s) 828
MscI TGGCCA 1 cut(s) 37
MseI TTAA 2 cut(s) 894, 1475
MslI CAYNNNNRTG 2 cut(s) 227, 530
Msp20I TGGCCA 1 cut(s) 37
MspR9I CCNGG 2 cut(s) 1435, 1594
MunI CAATTG 1 cut(s) 833
Mva1269I GAATGC 2 cut(s) 617, 698
MvaI CCWGG 2 cut(s) 1435, 1594
MvnI CGCG 1 cut(s) 1223
MwoI GCNNNNNNNGC 4 cut(s) 746, 758, 967, 1181
NarI GGCGCC 1 cut(s) 1364
NcoI CCATGG 3 cut(s) 249, 420, 525
NdeI CATATG 2 cut(s) 1110, 1369
NdeII GATC 5 cut(s) 282, 558, 643, 1011, 1317
NheI GCTAGC 1 cut(s) 1156
NlaIII CATG 9 cut(s) 124, 172, 253, 315, 424, 529, 803, 1144, 1221
NlaIV GGNNCC 1 cut(s) 1365
NmuCI GTSAC 4 cut(s) 220, 400, 883, 1205
NsiI ATGCAT 1 cut(s) 1219
NspI RCATGY 1 cut(s) 1221
PaeI GCATGC 1 cut(s) 1221
PaeR7I CTCGAG 1 cut(s) 1240
PcsI WCGNNNNNNNCGW 1 cut(s) 1313
PctI GAATGC 2 cut(s) 617, 698
PdmI GAANNNNTTC 1 cut(s) 828
PfeI GAWTC 3 cut(s) 264, 1304, 1406
PkrI GCNGC 2 cut(s) 354, 751
PleI GAGTC 4 cut(s) 393, 1232, 1256, 1303
PluTI GGCGCC 1 cut(s) 1367
PpsI GAGTC 4 cut(s) 393, 1232, 1256, 1303
Psp6I CCWGG 2 cut(s) 1433, 1592
PspGI CCWGG 2 cut(s) 1433, 1592
PspN4I GGNNCC 1 cut(s) 1365
PspXI VCTCGAGB 1 cut(s) 1240
PsuI RGATCY 1 cut(s) 1011
RsaI GTAC 1 cut(s) 1275
RsaNI GTAC 1 cut(s) 1274
RseI CAYNNNNRTG 2 cut(s) 227, 530
SaqAI TTAA 2 cut(s) 894, 1475
SatI GCNGC 2 cut(s) 353, 750
Sau3AI GATC 5 cut(s) 282, 558, 643, 1011, 1317
SchI GAGTC 4 cut(s) 394, 1232, 1256, 1304
ScrFI CCNGG 2 cut(s) 1435, 1594
SfaNI GCATC 5 cut(s) 134, 422, 557, 979, 1204
SfoI GGCGCC 1 cut(s) 1365
Sfr274I CTCGAG 1 cut(s) 1240
SlaI CTCGAG 1 cut(s) 1240
SmiMI CAYNNNNRTG 2 cut(s) 227, 530
SmlI CTYRAG 4 cut(s) 562, 605, 1240, 1380
SmoI CTYRAG 4 cut(s) 562, 605, 1240, 1380
SphI GCATGC 1 cut(s) 1221
SsiI CCGC 2 cut(s) 233, 759
SspDI GGCGCC 1 cut(s) 1363
SspI AATATT 1 cut(s) 720
SspMI CTAG 4 cut(s) 1079, 1157, 1325, 1609
StyD4I CCNGG 2 cut(s) 1433, 1592
StyI CCWWGG 3 cut(s) 249, 420, 525
TaaI ACNGT 1 cut(s) 936
TatI WGTACW 1 cut(s) 1273
TfiI GAWTC 3 cut(s) 264, 1304, 1406
Tru1I TTAA 2 cut(s) 894, 1475
Tru9I TTAA 2 cut(s) 894, 1475
TscAI CASTG 3 cut(s) 88, 331, 941
TseFI GTSAC 4 cut(s) 220, 400, 883, 1205
TseI GCWGC 2 cut(s) 352, 749
Tsp45I GTSAC 4 cut(s) 220, 400, 883, 1205
TspDTI ATGAA 4 cut(s) 32, 90, 109, 788
TspGWI ACGGA 2 cut(s) 275, 1224
TspRI CASTG 3 cut(s) 88, 331, 941
XapI RAATTY 9 cut(s) 55, 104, 182, 242, 685, 809, 1073, 1145, 1564
XceI RCATGY 1 cut(s) 1221
XcmI CCANNNNNNNNNTGG 1 cut(s) 1492
XhoI CTCGAG 1 cut(s) 1240
XmnI GAANNNNTTC 1 cut(s) 828
XspI CTAG 4 cut(s) 1079, 1157, 1325, 1609
Zsp2I ATGCAT 1 cut(s) 1219
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.