Rorug03G0055100

Belongs to the GRAS family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0004862
Physical Location & Seq
Reverse (-)
4244642 .. 4245211
570 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0055100.1

Sequence Viewer

Length: 570 bp
ATGGAACTATTTGATGTGGCATATATGGACGGAGCTTTCTATGGCGTTTTTTCAGACGGGATTTCAATAGTATTGCATGTCTTCACATTTAGAAGACGGAGCTTTCTATCGCGTCTTAAGAAAGCCATTGGACAAGCTGATGATGGTGAACAATGTGATCCATTCGTTATCTCCTCCTCCTCCTCTTCCTTAAGAAAGAAGGAGAATGATGATCTCTACTTAGTTGAGGCTGATGGGGATCTCTTATTAGCAATTTGTTCTATTGATGACACTCCCAGTTATTGTCACATATACCGGTTGGATCCGTCACGTAAAAAATGGAATCGAATTAGGAACTTGGGAAATCGAGTAATGATTTTGTCTTGCGATGGTGTTGGATCGTTTGTAGTTCCTGCAGTGCGAGATGCTAAAGAATTTGCAAATACGGTCTTTATTTATCGAGACTCAAGTAATGCTCATGCTATCTGTGAAAGTACTGAAGCCACTTGGATATTTGACAAAAAAATCCATCATGTAAATCCTTCTGAAGCAGGCACCACTCTCATTGAACCCCCTGACTTATGGCATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GRAS

Protein Analysis

189

Amino Acids

21.33

Weight (kDa)

5.47

Isoelectric Point (pI)

51.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_KIB1-4 PF03478 4 - 134 1.2e-12 KIB1-4 beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 533
AccII CGCG 1 cut(s) 112
AclWI GGATC 5 cut(s) 152, 246, 296, 309, 385
AcsI RAATTY 1 cut(s) 413
AcuI CTGAAG 2 cut(s) 498, 546
AfaI GTAC 1 cut(s) 475
AflII CTTAAG 2 cut(s) 116, 190
AgeI ACCGGT 1 cut(s) 294
AgsI TTSAA 2 cut(s) 66, 548
AluBI AGCT 3 cut(s) 35, 102, 137
AluI AGCT 3 cut(s) 35, 102, 137
Alw26I GTCTC 1 cut(s) 435
AlwI GGATC 5 cut(s) 152, 246, 296, 309, 385
ApoI RAATTY 1 cut(s) 413
AsiGI ACCGGT 1 cut(s) 294
AsuHPI GGTGA 1 cut(s) 158
BamHI GGATCC 1 cut(s) 301
BanI GGYRCC 1 cut(s) 533
BbsI GAAGAC 2 cut(s) 73, 100
BccI CCATC 4 cut(s) 137, 227, 362, 516
BcoDI GTCTC 1 cut(s) 435
BfmI CTRYAG 1 cut(s) 393
BfrI CTTAAG 2 cut(s) 116, 190
BmcAI AGTACT 1 cut(s) 475
BmiI GGNNCC 2 cut(s) 303, 535
BmrI ACTGGG 1 cut(s) 270
BmsI GCATC 1 cut(s) 394
BmuI ACTGGG 1 cut(s) 270
BpiI GAAGAC 2 cut(s) 73, 100
BpuEI CTTGAG 1 cut(s) 430
BsaAI YACGTR 1 cut(s) 311
BsaBI GATNNNNATC 1 cut(s) 237
BsaWI WCCGGW 1 cut(s) 294
Bse118I RCCGGY 1 cut(s) 294
Bse1I ACTGG 1 cut(s) 276
Bse8I GATNNNNATC 1 cut(s) 237
BseJI GATNNNNATC 1 cut(s) 237
BseNI ACTGG 1 cut(s) 276
BseRI GAGGAG 4 cut(s) 163, 166, 169, 172
Bsh1236I CGCG 1 cut(s) 112
BshNI GGYRCC 1 cut(s) 533
BshTI ACCGGT 1 cut(s) 294
BsiSI CCGG 1 cut(s) 295
BsmAI GTCTC 1 cut(s) 435
Bsp143I GATC 5 cut(s) 157, 211, 238, 301, 377
BspFNI CGCG 1 cut(s) 112
BspLI GGNNCC 2 cut(s) 303, 535
BspMAI CTGCAG 1 cut(s) 397
BspPI GGATC 5 cut(s) 152, 246, 296, 309, 385
BspT107I GGYRCC 1 cut(s) 533
BspTI CTTAAG 2 cut(s) 116, 190
BsrFI RCCGGY 1 cut(s) 294
BsrI ACTGG 1 cut(s) 276
BssAI RCCGGY 1 cut(s) 294
BssMI GATC 5 cut(s) 157, 211, 238, 301, 377
Bst4CI ACNGT 1 cut(s) 427
Bst6I CTCTTC 1 cut(s) 190
BstAFI CTTAAG 2 cut(s) 116, 190
BstBAI YACGTR 1 cut(s) 311
BstC8I GCNNGC 1 cut(s) 532
BstDEI CTNAG 1 cut(s) 220
BstFNI CGCG 1 cut(s) 112
BstKTI GATC 5 cut(s) 160, 214, 241, 304, 380
BstMAI GTCTC 1 cut(s) 435
BstMBI GATC 5 cut(s) 157, 211, 238, 301, 377
BstNSI RCATGY 1 cut(s) 80
BstSFI CTRYAG 1 cut(s) 393
BstUI CGCG 1 cut(s) 112
BstV2I GAAGAC 2 cut(s) 73, 100
BstX2I RGATCY 2 cut(s) 238, 301
BstYI RGATCY 2 cut(s) 238, 301
BtgZI GCGATG 1 cut(s) 381
BtsI GCAGTG 1 cut(s) 402
BtsIMutI CAGTG 1 cut(s) 402
Cac8I GCNNGC 1 cut(s) 532
Cfr10I RCCGGY 1 cut(s) 294
CseI GACGC 1 cut(s) 101
Csp6I GTAC 1 cut(s) 474
CspAI ACCGGT 1 cut(s) 294
CspCI CAANNNNNGTGG 2 cut(s) 526, 561
CviAII CATG 3 cut(s) 77, 458, 512
CviJI RGCY 6 cut(s) 35, 102, 125, 137, 230, 482
CviKI_1 RGCY 6 cut(s) 35, 102, 125, 137, 230, 482
CviQI GTAC 1 cut(s) 474
DdeI CTNAG 1 cut(s) 220
DpnI GATC 5 cut(s) 159, 213, 240, 303, 379
DpnII GATC 5 cut(s) 157, 211, 238, 301, 377
Eam1104I CTCTTC 1 cut(s) 190
EarI CTCTTC 1 cut(s) 190
Eco57I CTGAAG 2 cut(s) 498, 546
FaeI CATG 3 cut(s) 80, 461, 515
FatI CATG 3 cut(s) 76, 457, 511
HapII CCGG 1 cut(s) 295
HgaI GACGC 1 cut(s) 101
Hin1II CATG 3 cut(s) 80, 461, 515
HinfI GANTC 2 cut(s) 322, 443
HpaII CCGG 1 cut(s) 295
HphI GGTGA 1 cut(s) 158
Hpy166II GTNNAC 1 cut(s) 149
Hpy188I TCNGA 2 cut(s) 55, 526
Hpy188III TCNNGA 1 cut(s) 440
Hpy8I GTNNAC 1 cut(s) 149
HpyAV CCTTC 2 cut(s) 193, 531
HpyCH4III ACNGT 1 cut(s) 427
HpyCH4IV ACGT 1 cut(s) 310
HpyCH4V TGCA 3 cut(s) 76, 395, 419
HpyF3I CTNAG 1 cut(s) 220
HpySE526I ACGT 1 cut(s) 310
Hsp92II CATG 3 cut(s) 80, 461, 515
Kzo9I GATC 5 cut(s) 157, 211, 238, 301, 377
LmnI GCTCC 2 cut(s) 32, 99
LpnPI CCDG 4 cut(s) 289, 308, 405, 516
LweI GCATC 1 cut(s) 394
MaeII ACGT 1 cut(s) 310
MaeIII GTNAC 2 cut(s) 284, 306
MalI GATC 5 cut(s) 159, 213, 240, 303, 379
MboI GATC 5 cut(s) 157, 211, 238, 301, 377
MboII GAAGA 3 cut(s) 73, 105, 177
MflI RGATCY 2 cut(s) 238, 301
MluCI AATT 3 cut(s) 252, 327, 413
MlyI GAGTC 1 cut(s) 437
MmeI TCCRAC 2 cut(s) 279, 355
MnlI CCTC 5 cut(s) 184, 187, 190, 193, 220
MseI TTAA 2 cut(s) 117, 191
MspCI CTTAAG 2 cut(s) 116, 190
MspI CCGG 1 cut(s) 295
MvnI CGCG 1 cut(s) 112
NdeII GATC 5 cut(s) 157, 211, 238, 301, 377
NlaIII CATG 3 cut(s) 80, 461, 515
NlaIV GGNNCC 2 cut(s) 303, 535
NmuCI GTSAC 2 cut(s) 284, 306
NspI RCATGY 1 cut(s) 80
PfeI GAWTC 1 cut(s) 322
PinAI ACCGGT 1 cut(s) 294
PleI GAGTC 1 cut(s) 437
PpsI GAGTC 1 cut(s) 437
Ppu21I YACGTR 1 cut(s) 311
PspN4I GGNNCC 2 cut(s) 303, 535
PstI CTGCAG 1 cut(s) 397
PsuI RGATCY 2 cut(s) 238, 301
RsaI GTAC 1 cut(s) 475
RsaNI GTAC 1 cut(s) 474
SaqAI TTAA 2 cut(s) 117, 191
Sau3AI GATC 5 cut(s) 157, 211, 238, 301, 377
ScaI AGTACT 1 cut(s) 475
SchI GAGTC 1 cut(s) 437
SetI ASST 4 cut(s) 37, 104, 139, 313
SfaNI GCATC 1 cut(s) 394
SfcI CTRYAG 1 cut(s) 393
SmlI CTYRAG 3 cut(s) 116, 190, 445
SmoI CTYRAG 3 cut(s) 116, 190, 445
Sse9I AATT 3 cut(s) 252, 327, 413
TaaI ACNGT 1 cut(s) 427
TaiI ACGT 1 cut(s) 313
TaqI TCGA 3 cut(s) 325, 346, 439
TasI AATT 3 cut(s) 252, 327, 413
TatI WGTACW 1 cut(s) 473
TfiI GAWTC 1 cut(s) 322
Tru1I TTAA 2 cut(s) 117, 191
Tru9I TTAA 2 cut(s) 117, 191
TscAI CASTG 1 cut(s) 402
TseFI GTSAC 2 cut(s) 284, 306
Tsp45I GTSAC 2 cut(s) 284, 306
TspGWI ACGGA 3 cut(s) 45, 112, 294
TspRI CASTG 1 cut(s) 402
Vha464I CTTAAG 2 cut(s) 116, 190
XapI RAATTY 1 cut(s) 413
XceI RCATGY 1 cut(s) 80
ZrmI AGTACT 1 cut(s) 475
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.