FvH4_6g14570

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0002302
Physical Location & Seq
Forward (+)
8953959 .. 8957842
3884 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g14570.t1

Sequence Viewer

Length: 1299 bp
ATGGAGACGCAGAGGATAATTGAATTTCCTCATCAGAATATGGATAAGCGGCCCAGAAAGAGACCTCGATTAACTTGGGATATGCCTCCTCCTCTTCCTCCTCCCCAGCTGCTCCCAATATACTGCGGGCCGGAGTTTGGAAATGGGCCGATTCCCAATTATACATATACGTCTATGTTTTATGGGGGATTGCCTCGGAATGCCTCCCCTCCGTGGAGGCCTGATGATAAAGATGGTCATTATGTGTTTGGCATTGGAGATAATTTAACCCCTCGATATAGGATTCTCAACAAAATGGGAGAGGGGACTTTTGGGCAAGTGTTGGAGTGTTTGGACAATGAAAAGAAAGAACTTGTGGCTATTAAAATTATCCGCTCCATACATAAGTATCGTGAAGCTGCTATGATTGAAATTGATGTCCTACAAAGGCTTGCAAGGCATGATATTGGTGGCACTCGTTGTGTGCAAATTCGGAATTGGTTTGACTATCGTAATCATATTTGTATTGTATTTGAGAAGCTTGGACCTAGCTTATACGATTTTCTTCGCAAAAACAGCTACCGTTCATTTCCCATTGATCTTGTTCGGGAGCTTGCCAGACAACTTTTGGAGTCTGTAGCATTTATGCATGATTTACGCCTGATTCATACTGATTTGAAGCCGGAAAATATTCTGCTTGTTTCCTCCGACTACATCAAAGTGCCAGACTATAGGTTTCTAGCACGGTCAACTAAAGAGGGTTCTTATTTCAAGAATCTGCCCAAGTCAAGTGCCATCAAGCTCATTGATTTTGGAAGTACAACATTTGAACATCAGGATCATAGCTATGTGGTGTCAACACGCCATTATCGTGCACCTGAGGTTATTTTAGGTCTTGGGTGGAACTATCCTTGTGATATATGGAGTGTGGGCTGCATACTTGTTGAACTTTGCTCTGGTGAGGCCCTTTTTCAAACACATGAGAACTTGGAGCATCTTGCCATGATGGAGAGGGTGTTGGGACCACTGCCGCAGCATATGGTGCTCAGAGCTGACCGTCGTGCTGAGAAATATTTTAGGAGGGGAGCACGACTCGATTGGCCTGATGGGGCCGCATCTAGGGAAAGCATGCGAGCAGTATTCAAATTACCCAGATTACCGAACTTAATAATGCAACATGTGGATCACTCCGCTGGTGATCTTATTGAACTACTGCAAGGGCTCCTAAGATATGAGCCCACAGAGCGGCTCAAGGCAAGGGAAGCACTAAGACATCCCTTTTTCACTAGAGATGTGAGAAGGGGTGGCTATCCCCTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0006355 GO:0008150 GO:0009889 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:1903506 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

433

Amino Acids

50.3

Weight (kDa)

9.09

Isoelectric Point (pI)

46.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 93 - 421 2.6e-48 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 95 - 330 3.8e-19 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 2 cut(s) 375, 1225
AciI CCGC 7 cut(s) 49, 126, 373, 1010, 1092, 1170, 1225
AclWI GGATC 2 cut(s) 825, 1170
AcsI RAATTY 2 cut(s) 23, 468
AfaI GTAC 1 cut(s) 799
AfiI CCNNNNNNNGG 4 cut(s) 137, 213, 1098, 1224
AflIII ACRYGT 1 cut(s) 1156
AgsI TTSAA 9 cut(s) 23, 410, 658, 751, 809, 926, 953, 1123, 1187
AluBI AGCT 9 cut(s) 109, 398, 520, 531, 558, 592, 781, 825, 1031
AluI AGCT 9 cut(s) 109, 398, 520, 531, 558, 592, 781, 825, 1031
Alw21I GWGCWC 3 cut(s) 856, 1026, 1069
Alw26I GTCTC 1 cut(s) 55
Alw44I GTGCAC 1 cut(s) 852
AlwI GGATC 2 cut(s) 825, 1170
AoxI GGCC 7 cut(s) 50, 128, 146, 218, 942, 1079, 1089
ApaLI GTGCAC 1 cut(s) 852
ApeKI GCWGC 4 cut(s) 109, 398, 912, 1012
ApoI RAATTY 2 cut(s) 23, 468
Asp700I GAANNNNTTC 1 cut(s) 669
AspS9I GGNCC 7 cut(s) 51, 128, 146, 524, 943, 1001, 1089
AsuHPI GGTGA 2 cut(s) 950, 1187
AvaII GGWCC 2 cut(s) 524, 1001
AxyI CCTNAGG 1 cut(s) 858
BaeGI GKGCMC 1 cut(s) 856
BaeI ACNNNNGTAYC 2 cut(s) 371, 404
BanII GRGCYC 2 cut(s) 1203, 1218
Bbv12I GWGCWC 3 cut(s) 856, 1026, 1069
BbvI GCAGC 4 cut(s) 96, 385, 899, 1024
BccI CCATC 4 cut(s) 227, 782, 979, 1079
BcoDI GTCTC 1 cut(s) 55
BfaI CTAG 4 cut(s) 528, 719, 1098, 1266
BfmI CTRYAG 2 cut(s) 615, 709
BisI GCNGC 8 cut(s) 50, 110, 399, 913, 1010, 1013, 1092, 1226
BlsI GCNGC 8 cut(s) 51, 111, 400, 914, 1011, 1014, 1093, 1227
Bme18I GGWCC 2 cut(s) 524, 1001
BmgT120I GGNCC 7 cut(s) 51, 128, 146, 524, 943, 1001, 1089
BmiI GGNNCC 3 cut(s) 1002, 1090, 1202
BmsI GCATC 2 cut(s) 982, 1103
BplI GAGNNNNNCTC 2 cut(s) 1056, 1088
BpuEI CTTGAG 1 cut(s) 1214
BsaI GGTCTC 1 cut(s) 55
BsaJI CCNNGG 2 cut(s) 194, 212
BsaXI ACNNNNNCTCC 2 cut(s) 980, 1010
Bsc4I CCNNNNNNNGG 4 cut(s) 137, 213, 1098, 1224
Bse21I CCTNAGG 1 cut(s) 858
BseDI CCNNGG 2 cut(s) 194, 212
BseGI GGATG 1 cut(s) 1252
BseLI CCNNNNNNNGG 4 cut(s) 137, 213, 1098, 1224
BseMII CTCAG 3 cut(s) 849, 1035, 1039
BseRI GAGGAG 3 cut(s) 78, 81, 90
BseSI GKGCMC 1 cut(s) 856
BseXI GCAGC 4 cut(s) 96, 385, 899, 1024
BseYI CCCAGC 1 cut(s) 105
BshFI GGCC 7 cut(s) 52, 130, 148, 220, 944, 1081, 1091
BsiHKAI GWGCWC 3 cut(s) 856, 1026, 1069
BsiSI CCGG 2 cut(s) 131, 662
BslFI GGGAC 2 cut(s) 319, 1014
BslI CCNNNNNNNGG 4 cut(s) 137, 213, 1098, 1224
BsmAI GTCTC 1 cut(s) 55
BsmFI GGGAC 2 cut(s) 319, 1014
BsmI GAATGC 1 cut(s) 205
BsnI GGCC 7 cut(s) 52, 130, 148, 220, 944, 1081, 1091
Bso31I GGTCTC 1 cut(s) 55
Bsp1286I GDGCHC 5 cut(s) 856, 1026, 1069, 1203, 1218
Bsp143I GATC 4 cut(s) 577, 817, 1162, 1177
BspACI CCGC 7 cut(s) 49, 126, 373, 1010, 1092, 1170, 1225
BspANI GGCC 7 cut(s) 52, 130, 148, 220, 944, 1081, 1091
BspCNI CTCAG 3 cut(s) 850, 1036, 1038
BspLI GGNNCC 3 cut(s) 1002, 1090, 1202
BspPI GGATC 2 cut(s) 825, 1170
BspTNI GGTCTC 1 cut(s) 55
BsrBI CCGCTC 2 cut(s) 375, 1225
BssECI CCNNGG 2 cut(s) 194, 212
BssMI GATC 4 cut(s) 577, 817, 1162, 1177
Bst4CI ACNGT 3 cut(s) 563, 726, 1037
Bst6I CTCTTC 1 cut(s) 99
BstAPI GCANNNNNTGC 1 cut(s) 1021
BstC8I GCNNGC 5 cut(s) 128, 432, 594, 1109, 1113
BstDEI CTNAG 5 cut(s) 858, 1025, 1044, 1205, 1247
BstDSI CCRYGG 1 cut(s) 212
BstF5I GGATG 1 cut(s) 1252
BstKTI GATC 4 cut(s) 580, 820, 1165, 1180
BstMAI GTCTC 1 cut(s) 55
BstMBI GATC 4 cut(s) 577, 817, 1162, 1177
BstMWI GCNNNNNNNGC 5 cut(s) 436, 555, 1021, 1222, 1241
BstNSI RCATGY 2 cut(s) 1111, 1160
BstSFI CTRYAG 2 cut(s) 615, 709
BstSLI GKGCMC 1 cut(s) 856
BstV1I GCAGC 4 cut(s) 96, 385, 899, 1024
Bsu36I CCTNAGG 1 cut(s) 858
BsuRI GGCC 7 cut(s) 52, 130, 148, 220, 944, 1081, 1091
BtgI CCRYGG 1 cut(s) 212
BtsCI GGATG 1 cut(s) 1252
BtsI GCAGTG 1 cut(s) 1004
BtsIMutI CAGTG 1 cut(s) 1004
Cac8I GCNNGC 5 cut(s) 128, 432, 594, 1109, 1113
Cfr13I GGNCC 7 cut(s) 51, 128, 146, 524, 943, 1001, 1089
CseI GACGC 1 cut(s) 16
Csp6I GTAC 1 cut(s) 798
CviAII CATG 6 cut(s) 440, 629, 959, 982, 1108, 1157
CviQI GTAC 1 cut(s) 798
DdeI CTNAG 5 cut(s) 858, 1025, 1044, 1205, 1247
DpnI GATC 4 cut(s) 579, 819, 1164, 1179
DpnII GATC 4 cut(s) 577, 817, 1162, 1177
Eam1104I CTCTTC 1 cut(s) 99
EarI CTCTTC 1 cut(s) 99
Eco147I AGGCCT 1 cut(s) 220
Eco24I GRGCYC 2 cut(s) 1203, 1218
Eco31I GGTCTC 1 cut(s) 55
Eco47I GGWCC 2 cut(s) 524, 1001
Eco81I CCTNAGG 1 cut(s) 858
EcoO109I RGGNCCY 1 cut(s) 943
EcoT22I ATGCAT 1 cut(s) 630
EcoT38I GRGCYC 2 cut(s) 1203, 1218
FaeI CATG 6 cut(s) 443, 632, 962, 985, 1111, 1160
FaqI GGGAC 2 cut(s) 319, 1014
FatI CATG 6 cut(s) 439, 628, 958, 981, 1107, 1156
FauI CCCGC 1 cut(s) 119
FauNDI CATATG 1 cut(s) 1017
Fnu4HI GCNGC 8 cut(s) 50, 110, 399, 913, 1010, 1013, 1092, 1226
FokI GGATG 1 cut(s) 1239
FriOI GRGCYC 2 cut(s) 1203, 1218
Fsp4HI GCNGC 8 cut(s) 50, 110, 399, 913, 1010, 1013, 1092, 1226
FspBI CTAG 4 cut(s) 528, 719, 1098, 1266
GluI GCNGC 8 cut(s) 50, 110, 399, 913, 1010, 1013, 1092, 1226
GsaI CCCAGC 1 cut(s) 109
HaeIII GGCC 7 cut(s) 52, 130, 148, 220, 944, 1081, 1091
HapII CCGG 2 cut(s) 131, 662
HgaI GACGC 1 cut(s) 16
Hin1II CATG 6 cut(s) 443, 632, 962, 985, 1111, 1160
HincII GTYRAC 2 cut(s) 729, 837
HindII GTYRAC 2 cut(s) 729, 837
HindIII AAGCTT 1 cut(s) 518
HinfI GANTC 6 cut(s) 151, 283, 611, 643, 754, 1071
HpaII CCGG 2 cut(s) 131, 662
HphI GGTGA 2 cut(s) 950, 1187
Hpy166II GTNNAC 3 cut(s) 729, 837, 854
Hpy188I TCNGA 5 cut(s) 36, 198, 474, 688, 1028
Hpy188III TCNNGA 4 cut(s) 392, 587, 751, 815
Hpy8I GTNNAC 3 cut(s) 729, 837, 854
Hpy99I CGWCG 1 cut(s) 1041
HpyAV CCTTC 1 cut(s) 1272
HpyCH4III ACNGT 3 cut(s) 563, 726, 1037
HpyCH4IV ACGT 1 cut(s) 170
HpyCH4V TGCA 7 cut(s) 434, 466, 628, 854, 915, 1153, 1195
HpyF10VI GCNNNNNNNGC 5 cut(s) 436, 555, 1021, 1222, 1241
HpyF3I CTNAG 5 cut(s) 858, 1025, 1044, 1205, 1247
HpySE526I ACGT 1 cut(s) 170
Hsp92II CATG 6 cut(s) 443, 632, 962, 985, 1111, 1160
Kzo9I GATC 4 cut(s) 577, 817, 1162, 1177
LmnI GCTCC 6 cut(s) 117, 380, 589, 970, 1064, 1206
Lsp1109I GCAGC 4 cut(s) 96, 385, 899, 1024
LweI GCATC 2 cut(s) 982, 1103
MaeI CTAG 4 cut(s) 528, 719, 1098, 1266
MaeII ACGT 1 cut(s) 170
MalI GATC 4 cut(s) 579, 819, 1164, 1179
MbiI CCGCTC 2 cut(s) 375, 1225
MboI GATC 4 cut(s) 577, 817, 1162, 1177
MboII GAAGA 2 cut(s) 86, 536
MhlI GDGCHC 5 cut(s) 856, 1026, 1069, 1203, 1218
MluCI AATT 9 cut(s) 18, 23, 157, 262, 366, 411, 468, 475, 1124
MlyI GAGTC 2 cut(s) 620, 1065
MmeI TCCRAC 2 cut(s) 303, 711
Mph1103I ATGCAT 1 cut(s) 630
MroXI GAANNNNTTC 1 cut(s) 669
MseI TTAA 5 cut(s) 71, 266, 363, 1145, 1297
MslI CAYNNNNRTG 3 cut(s) 698, 825, 849
MspA1I CMGCKG 2 cut(s) 109, 1172
MspI CCGG 2 cut(s) 131, 662
Mva1269I GAATGC 1 cut(s) 205
MwoI GCNNNNNNNGC 5 cut(s) 436, 555, 1021, 1222, 1241
NdeI CATATG 1 cut(s) 1017
NdeII GATC 4 cut(s) 577, 817, 1162, 1177
NlaIII CATG 6 cut(s) 443, 632, 962, 985, 1111, 1160
NlaIV GGNNCC 3 cut(s) 1002, 1090, 1202
NsiI ATGCAT 1 cut(s) 630
NspI RCATGY 2 cut(s) 1111, 1160
PaeI GCATGC 1 cut(s) 1111
PceI AGGCCT 1 cut(s) 220
PciI ACATGT 1 cut(s) 1156
PcsI WCGNNNNNNNCGW 1 cut(s) 847
PctI GAATGC 1 cut(s) 205
PdmI GAANNNNTTC 1 cut(s) 669
PfeI GAWTC 4 cut(s) 151, 283, 643, 754
PkrI GCNGC 8 cut(s) 51, 111, 400, 914, 1011, 1014, 1093, 1227
PleI GAGTC 2 cut(s) 619, 1065
PpsI GAGTC 2 cut(s) 619, 1065
PscI ACATGT 1 cut(s) 1156
PspFI CCCAGC 1 cut(s) 105
PspN4I GGNNCC 3 cut(s) 1002, 1090, 1202
PspPI GGNCC 7 cut(s) 51, 128, 146, 524, 943, 1001, 1089
PvuII CAGCTG 1 cut(s) 109
RsaI GTAC 1 cut(s) 799
RsaNI GTAC 1 cut(s) 798
RseI CAYNNNNRTG 3 cut(s) 698, 825, 849
SaqAI TTAA 5 cut(s) 71, 266, 363, 1145, 1297
SatI GCNGC 8 cut(s) 50, 110, 399, 913, 1010, 1013, 1092, 1226
Sau3AI GATC 4 cut(s) 577, 817, 1162, 1177
Sau96I GGNCC 7 cut(s) 51, 128, 146, 524, 943, 1001, 1089
SchI GAGTC 2 cut(s) 620, 1065
SduI GDGCHC 5 cut(s) 856, 1026, 1069, 1203, 1218
SfaNI GCATC 2 cut(s) 982, 1103
SfcI CTRYAG 2 cut(s) 615, 709
SinI GGWCC 2 cut(s) 524, 1001
SmiMI CAYNNNNRTG 3 cut(s) 698, 825, 849
SmlI CTYRAG 1 cut(s) 1229
SmoI CTYRAG 1 cut(s) 1229
SphI GCATGC 1 cut(s) 1111
Sse9I AATT 9 cut(s) 18, 23, 157, 262, 366, 411, 468, 475, 1124
SseBI AGGCCT 1 cut(s) 220
SsiI CCGC 7 cut(s) 49, 126, 373, 1010, 1092, 1170, 1225
SspI AATATT 2 cut(s) 670, 1052
SspMI CTAG 4 cut(s) 528, 719, 1098, 1266
StuI AGGCCT 1 cut(s) 220
TaaI ACNGT 3 cut(s) 563, 726, 1037
TaiI ACGT 1 cut(s) 173
TaqI TCGA 3 cut(s) 67, 274, 1074
TasI AATT 9 cut(s) 18, 23, 157, 262, 366, 411, 468, 475, 1124
TatI WGTACW 1 cut(s) 797
TauI GCSGC 4 cut(s) 52, 1012, 1094, 1228
TfiI GAWTC 4 cut(s) 151, 283, 643, 754
Tru1I TTAA 5 cut(s) 71, 266, 363, 1145, 1297
Tru9I TTAA 5 cut(s) 71, 266, 363, 1145, 1297
TscAI CASTG 1 cut(s) 1011
TseI GCWGC 4 cut(s) 109, 398, 912, 1012
TspDTI ATGAA 3 cut(s) 354, 555, 635
TspGWI ACGGA 1 cut(s) 201
TspRI CASTG 1 cut(s) 1011
VneI GTGCAC 1 cut(s) 852
VpaK11BI GGWCC 2 cut(s) 524, 1001
XapI RAATTY 2 cut(s) 23, 468
XceI RCATGY 2 cut(s) 1111, 1160
XcmI CCANNNNNNNNNTGG 1 cut(s) 604
XmnI GAANNNNTTC 1 cut(s) 669
XspI CTAG 4 cut(s) 528, 719, 1098, 1266
Zsp2I ATGCAT 1 cut(s) 630
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.