Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0002302

Physical Location & Seq

Genomic Coordinates Reverse (-)

21311367 .. 21314752

Gene Structure 3386 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1136400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1302 bp

ATGGAAACGCAGAGGATAATGGAATTTCCTCACAAGAATATGGATAAGCGGCCGAGAAAGAGACCTCGATTAGCATGGGATATGCCCCCTCCTCTTCCTCCTCCCGAGCTGCTCCCGGCATTTTATTGCGGGCAGGAGTTTGGGAATGGGCCGATCCCGAATTACACATATACGTCTATGTTTTATGGGGGAGTGCCTCGAAATGCTTCCCCTCCATGGAGACCTGATGATAAAGATGGTCATTACGTGTTTGGCATCGGAGAAAATCTAACCCCTCGATATAGAATTCTCAGCAAAATGGGAGAGGGGACTTTTGGGCAAGTGTTAGAGTGTATTGACAACGAAAAGAAAGAACTTGTGGCAATCAAAATTGTCCGCTCCATACATAAGTATCGTGAAGCTGCGATGATTGAAATTGATGTCCTACAAAGGCTTGCAAGGCATGATATTGGTGGCACTCGTTGTGTGCAAATACGGAATTGGTTTGACTATCGTAATCATATTTGTATTGTGTTTGAGAAGCTTGGACCTAGCTTATACGATTTTCTTCGCAAAAACAGCTATCGTTCATTTCCCATTGATCTTGTTCGGGAGCTTGGCAGACAACTTTTGGAGTCTGTAGCATTTATGCATGATTTACGCCTTATTCATACTGATTTGAAGCCAGAGAATATTCTGCTTGTGTCCTCCGACTACATTAAAGTGCCAGACTATAAGTTTCTATCACGTTGGACTAAAGAGGGTTCCTATTTCAAGAATCTGCCCAAGTCAAGTGCCATTAAGCTCATTGATTTTGGGAGTACAACATTTGAACATCAGGATCACAGCTATGTGGTGTCAACACGCCATTATCGTGCACCAGAGGTCATTTTGGGACTTGGATGGAATTATCCTTGTGATATATGGAGCATTGGCTGCATACTTGTTGAACTTTGCTCGGGTGAGGCCCTTTTTCAAACACATGAAAACTTGGAGCATCTTGCCATGATGGAGAGGGTTTTGGGCCCACTACCACAACATATGGTGGTCAGAGCCGACCGTCGTGCTGACAAATATTTTAGAAGGGGAGCACGACTAGATTGGCCTGACGGTGCAACTTCTAGAGAAAGCATGAGAGCAGTTTGCAAGTTGCCCCGGCTTCCGAACCTAATAATGCAGCATGTGGACCACTCTGCTGGTGATTTGATCGAGCTCTTGCAAGGGCTCCTAAGATACGACCCGGCAGAGCGCCTCAAGGCTAGGGAAGCTCTAAGACATCCCTTCTTCACTAGAGATATGAGAAGGGGTGGCTACCCCTTGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0006355 GO:0008150 GO:0009889 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pkinase

Protein Analysis

434

Amino Acids

50.41

Weight (kDa)

9.03

Isoelectric Point (pI)

46.92

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	94 - 422	2.6e-48	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	96 - 331	1e-18	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002302)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G53570 AT3G53570 AT3G53570 AT3G53570 AT3G53570
fragaria_vesca	FvH4_4g25400 FvH4_4g25400 FvH4_4g25400 FvH4_6g14570 FvH4_6g14570 FvH4_6g14570 FvH4_6g14570
malus_domestica	MD12G1136400.v1.1 MD16G1103500.v1.1
prunus_persica	Prupe.1G242500_v2.0.a1 Prupe.1G242500_v2.0.a1 Prupe.6G248800_v2.0.a1 Prupe.6G248800_v2.0.a1 Prupe.6G248800_v2.0.a1
pyrus_communis	pycom13g09030
rosa_chinensis	RchiOBHm_Chr3g0467621 RchiOBHm_Chr4g0432711
rosa_laevigata	RLG00000006818 RLG00000024484
rosa_multiflora	Rmu_co8522421.1_g000003 Rmu_sc0012180.1_g000012
rosa_roxburghii	Rroxscaffold_5G00374170 Rroxscaffold_6G00413540
rosa_rugosa	Rorug03G0088600 Rorug04G0261900 Rorug04G0262000 Rorug04G0262000
rosa_samantha	Rh3AG136900 Rh3BG158700 Rh3CG158300 Rh3DG159200 Rh4AG316300 Rh4BG324300 Rh4CG339300 Rh4DG319600
rosa_wichuraiana	Rw3G012970 Rw4G027510

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccBSI	CCGCTC	1 cut(s)	378
AciI	CCGC	3 cut(s)	49, 129, 376
AclWI	GGATC	2 cut(s)	148, 828
AcoI	YGGCCR	1 cut(s)	50
AcsI	RAATTY	2 cut(s)	23, 285
AfaI	GTAC	1 cut(s)	802
AfiI	CCNNNNNNNGG	1 cut(s)	216
AflIII	ACRYGT	1 cut(s)	246
AgsI	TTSAA	6 cut(s)	413, 661, 754, 812, 929, 956
Alw21I	GWGCWC	3 cut(s)	859, 1072, 1194
Alw26I	GTCTC	2 cut(s)	55, 214
Alw44I	GTGCAC	1 cut(s)	855
AlwI	GGATC	2 cut(s)	148, 828
Ama87I	CYCGRG	2 cut(s)	104, 937
AoxI	GGCC	5 cut(s)	50, 149, 945, 1003, 1082
ApaI	GGGCCC	1 cut(s)	1007
ApaLI	GTGCAC	1 cut(s)	855
ApeKI	GCWGC	4 cut(s)	109, 401, 915, 1156
ApoI	RAATTY	2 cut(s)	23, 285
Asp700I	GAANNNNTTC	1 cut(s)	205
AspLEI	GCGC	1 cut(s)	1230
AspS9I	GGNCC	6 cut(s)	149, 527, 946, 1003, 1004, 1165
AsuC2I	CCSGG	3 cut(s)	116, 1135, 1220
AsuHPI	GGTGA	2 cut(s)	953, 1190
AvaI	CYCGRG	2 cut(s)	104, 937
AvaII	GGWCC	2 cut(s)	527, 1165
BaeGI	GKGCMC	2 cut(s)	859, 1007
BaeI	ACNNNNGTAYC	2 cut(s)	374, 407
BanII	GRGCYC	3 cut(s)	1007, 1194, 1206
Bbv12I	GWGCWC	3 cut(s)	859, 1072, 1194
BbvI	GCAGC	4 cut(s)	96, 388, 902, 1168
BccI	CCATC	3 cut(s)	230, 876, 982
BcgI	CGANNNNNNTGC	2 cut(s)	1025, 1059
BcnI	CCSGG	3 cut(s)	116, 1135, 1220
BcoDI	GTCTC	2 cut(s)	55, 214
BfaI	CTAG	5 cut(s)	531, 1076, 1101, 1239, 1269
BfmI	CTRYAG	1 cut(s)	618
BfoI	RGCGCY	1 cut(s)	1231
BisI	GCNGC	5 cut(s)	50, 110, 402, 916, 1157
BlsI	GCNGC	5 cut(s)	51, 111, 403, 917, 1158
Bme1390I	CCNGG	3 cut(s)	116, 1135, 1220
Bme18I	GGWCC	2 cut(s)	527, 1165
BmeT110I	CYCGRG	2 cut(s)	104, 937
BmgT120I	GGNCC	6 cut(s)	149, 527, 946, 1003, 1004, 1165
BmiI	GGNNCC	3 cut(s)	745, 1005, 1205
BmrFI	CCNGG	3 cut(s)	116, 1135, 1220
BmsI	GCATC	2 cut(s)	264, 985
BpuEI	CTTGAG	1 cut(s)	1217
BpuMI	CCSGG	3 cut(s)	116, 1135, 1220
BsaAI	YACGTR	1 cut(s)	247
BsaI	GGTCTC	2 cut(s)	55, 214
BsaJI	CCNNGG	2 cut(s)	215, 1133
Bsc4I	CCNNNNNNNGG	1 cut(s)	216
BseDI	CCNNGG	2 cut(s)	215, 1133
BseGI	GGATG	2 cut(s)	887, 1255
BseLI	CCNNNNNNNGG	1 cut(s)	216
BseMII	CTCAG	1 cut(s)	304
BseRI	GAGGAG	2 cut(s)	81, 90
BseSI	GKGCMC	2 cut(s)	859, 1007
BseX3I	CGGCCG	1 cut(s)	50
BseXI	GCAGC	4 cut(s)	96, 388, 902, 1168
Bsh1285I	CGRYCG	2 cut(s)	53, 1039
BshFI	GGCC	5 cut(s)	52, 151, 947, 1005, 1084
BsiEI	CGRYCG	2 cut(s)	53, 1039
BsiHKAI	GWGCWC	3 cut(s)	859, 1072, 1194
BsiHKCI	CYCGRG	2 cut(s)	104, 937
BsiSI	CCGG	3 cut(s)	116, 1135, 1220
BslFI	GGGAC	2 cut(s)	322, 888
BslI	CCNNNNNNNGG	1 cut(s)	216
BsmAI	GTCTC	2 cut(s)	55, 214
BsmFI	GGGAC	2 cut(s)	322, 888
BsnI	GGCC	5 cut(s)	52, 151, 947, 1005, 1084
Bso31I	GGTCTC	2 cut(s)	55, 214
BsoBI	CYCGRG	2 cut(s)	104, 937
Bsp120I	GGGCCC	1 cut(s)	1003
Bsp1286I	GDGCHC	5 cut(s)	859, 1007, 1072, 1194, 1206
Bsp143I	GATC	4 cut(s)	153, 580, 820, 1185
Bsp19I	CCATGG	1 cut(s)	215
BspACI	CCGC	3 cut(s)	49, 129, 376
BspANI	GGCC	5 cut(s)	52, 151, 947, 1005, 1084
BspCNI	CTCAG	1 cut(s)	303
BspLI	GGNNCC	3 cut(s)	745, 1005, 1205
BspPI	GGATC	2 cut(s)	148, 828
BspTNI	GGTCTC	2 cut(s)	55, 214
BsrBI	CCGCTC	1 cut(s)	378
BssECI	CCNNGG	2 cut(s)	215, 1133
BssMI	GATC	4 cut(s)	153, 580, 820, 1185
BssT1I	CCWWGG	1 cut(s)	215
Bst4CI	ACNGT	2 cut(s)	1040, 1091
Bst6I	CTCTTC	1 cut(s)	99
BstAPI	GCANNNNNTGC	1 cut(s)	915
BstBAI	YACGTR	1 cut(s)	247
BstC8I	GCNNGC	2 cut(s)	131, 435
BstDEI	CTNAG	3 cut(s)	290, 1208, 1250
BstDSI	CCRYGG	1 cut(s)	215
BstF5I	GGATG	2 cut(s)	887, 1255
BstH2I	RGCGCY	1 cut(s)	1231
BstHHI	GCGC	1 cut(s)	1230
BstKTI	GATC	4 cut(s)	156, 583, 823, 1188
BstMAI	GTCTC	2 cut(s)	55, 214
BstMBI	GATC	4 cut(s)	153, 580, 820, 1185
BstMCI	CGRYCG	2 cut(s)	53, 1039
BstMWI	GCNNNNNNNGC	4 cut(s)	439, 558, 915, 1244
BstNSI	RCATGY	1 cut(s)	1163
BstSCI	CCNGG	3 cut(s)	114, 1133, 1218
BstSFI	CTRYAG	1 cut(s)	618
BstSLI	GKGCMC	2 cut(s)	859, 1007
BstV1I	GCAGC	4 cut(s)	96, 388, 902, 1168
BstXI	CCANNNNNNTGG	1 cut(s)	1175
BstZI	CGGCCG	1 cut(s)	50
BsuRI	GGCC	5 cut(s)	52, 151, 947, 1005, 1084
BtgI	CCRYGG	1 cut(s)	215
BtgZI	GCGATG	1 cut(s)	419
BtsCI	GGATG	2 cut(s)	887, 1255
Cac8I	GCNNGC	2 cut(s)	131, 435
CfoI	GCGC	1 cut(s)	1230
Cfr13I	GGNCC	6 cut(s)	149, 527, 946, 1003, 1004, 1165
Csp6I	GTAC	1 cut(s)	801
CviAII	CATG	8 cut(s)	75, 216, 443, 632, 962, 985, 1111, 1160
CviQI	GTAC	1 cut(s)	801
DdeI	CTNAG	3 cut(s)	290, 1208, 1250
DpnI	GATC	4 cut(s)	155, 582, 822, 1187
DpnII	GATC	4 cut(s)	153, 580, 820, 1185
EaeI	YGGCCR	1 cut(s)	50
EagI	CGGCCG	1 cut(s)	50
Eam1104I	CTCTTC	1 cut(s)	99
EarI	CTCTTC	1 cut(s)	99
Ecl136II	GAGCTC	1 cut(s)	1192
EclXI	CGGCCG	1 cut(s)	50
Eco130I	CCWWGG	1 cut(s)	215
Eco24I	GRGCYC	3 cut(s)	1007, 1194, 1206
Eco31I	GGTCTC	2 cut(s)	55, 214
Eco47I	GGWCC	2 cut(s)	527, 1165
Eco52I	CGGCCG	1 cut(s)	50
Eco53kI	GAGCTC	1 cut(s)	1192
Eco88I	CYCGRG	2 cut(s)	104, 937
EcoICRI	GAGCTC	1 cut(s)	1192
EcoO109I	RGGNCCY	1 cut(s)	946
EcoRI	GAATTC	1 cut(s)	285
EcoT14I	CCWWGG	1 cut(s)	215
EcoT22I	ATGCAT	1 cut(s)	633
EcoT38I	GRGCYC	3 cut(s)	1007, 1194, 1206
ErhI	CCWWGG	1 cut(s)	215
FaeI	CATG	8 cut(s)	78, 219, 446, 635, 965, 988, 1114, 1163
FaqI	GGGAC	2 cut(s)	322, 888
FatI	CATG	8 cut(s)	74, 215, 442, 631, 961, 984, 1110, 1159
FauI	CCCGC	1 cut(s)	122
FauNDI	CATATG	1 cut(s)	1020
Fnu4HI	GCNGC	5 cut(s)	50, 110, 402, 916, 1157
FokI	GGATG	2 cut(s)	894, 1242
FriOI	GRGCYC	3 cut(s)	1007, 1194, 1206
Fsp4HI	GCNGC	5 cut(s)	50, 110, 402, 916, 1157
FspBI	CTAG	5 cut(s)	531, 1076, 1101, 1239, 1269
GlaI	GCGC	1 cut(s)	1229
GluI	GCNGC	5 cut(s)	50, 110, 402, 916, 1157
HaeII	RGCGCY	1 cut(s)	1231
HaeIII	GGCC	5 cut(s)	52, 151, 947, 1005, 1084
HapII	CCGG	3 cut(s)	116, 1135, 1220
HhaI	GCGC	1 cut(s)	1230
Hin1II	CATG	8 cut(s)	78, 219, 446, 635, 965, 988, 1114, 1163
Hin6I	GCGC	1 cut(s)	1228
HinP1I	GCGC	1 cut(s)	1228
HincII	GTYRAC	1 cut(s)	840
HindII	GTYRAC	1 cut(s)	840
HindIII	AAGCTT	1 cut(s)	521
HinfI	GANTC	2 cut(s)	614, 757
HpaII	CCGG	3 cut(s)	116, 1135, 1220
HphI	GGTGA	2 cut(s)	953, 1190
Hpy166II	GTNNAC	3 cut(s)	840, 857, 1165
Hpy188I	TCNGA	4 cut(s)	260, 691, 1031, 1143
Hpy188III	TCNNGA	7 cut(s)	104, 157, 395, 590, 754, 818, 1101
Hpy8I	GTNNAC	3 cut(s)	840, 857, 1165
Hpy99I	CGWCG	1 cut(s)	1044
HpyAV	CCTTC	3 cut(s)	1056, 1270, 1275
HpyCH4III	ACNGT	2 cut(s)	1040, 1091
HpyCH4IV	ACGT	3 cut(s)	173, 246, 727
HpyCH4V	TGCA	9 cut(s)	437, 469, 631, 857, 918, 1094, 1125, 1156, 1198
HpyF10VI	GCNNNNNNNGC	4 cut(s)	439, 558, 915, 1244
HpyF3I	CTNAG	3 cut(s)	290, 1208, 1250
HpySE526I	ACGT	3 cut(s)	173, 246, 727
Hsp92II	CATG	8 cut(s)	78, 219, 446, 635, 965, 988, 1114, 1163
HspAI	GCGC	1 cut(s)	1228
Kzo9I	GATC	4 cut(s)	153, 580, 820, 1185
LmnI	GCTCC	7 cut(s)	117, 383, 592, 906, 973, 1067, 1209
Lsp1109I	GCAGC	4 cut(s)	96, 388, 902, 1168
LweI	GCATC	2 cut(s)	264, 985
MaeI	CTAG	5 cut(s)	531, 1076, 1101, 1239, 1269
MaeII	ACGT	3 cut(s)	173, 246, 727
MalI	GATC	4 cut(s)	155, 582, 822, 1187
MbiI	CCGCTC	1 cut(s)	378
MboI	GATC	4 cut(s)	153, 580, 820, 1185
MboII	GAAGA	3 cut(s)	86, 539, 1255
MhlI	GDGCHC	5 cut(s)	859, 1007, 1072, 1194, 1206
MluCI	AATT	7 cut(s)	23, 160, 285, 369, 414, 478, 886
MlyI	GAGTC	1 cut(s)	623
MmeI	TCCRAC	2 cut(s)	710, 714
Mph1103I	ATGCAT	1 cut(s)	633
MroXI	GAANNNNTTC	1 cut(s)	205
MseI	TTAA	2 cut(s)	699, 780
MslI	CAYNNNNRTG	3 cut(s)	701, 828, 852
MspI	CCGG	3 cut(s)	116, 1135, 1220
MspR9I	CCNGG	3 cut(s)	116, 1135, 1220
MwoI	GCNNNNNNNGC	4 cut(s)	439, 558, 915, 1244
NciI	CCSGG	3 cut(s)	116, 1135, 1220
NcoI	CCATGG	1 cut(s)	215
NdeI	CATATG	1 cut(s)	1020
NdeII	GATC	4 cut(s)	153, 580, 820, 1185
NlaIII	CATG	8 cut(s)	78, 219, 446, 635, 965, 988, 1114, 1163
NlaIV	GGNNCC	3 cut(s)	745, 1005, 1205
NmeAIII	GCCGAG	1 cut(s)	78
NsiI	ATGCAT	1 cut(s)	633
NspI	RCATGY	1 cut(s)	1163
PcsI	WCGNNNNNNNCGW	1 cut(s)	850
PdmI	GAANNNNTTC	1 cut(s)	205
PfeI	GAWTC	1 cut(s)	757
PkrI	GCNGC	5 cut(s)	51, 111, 403, 917, 1158
PleI	GAGTC	1 cut(s)	622
PpsI	GAGTC	1 cut(s)	622
Ppu21I	YACGTR	1 cut(s)	247
Psp124BI	GAGCTC	1 cut(s)	1194
PspN4I	GGNNCC	3 cut(s)	745, 1005, 1205
PspOMI	GGGCCC	1 cut(s)	1003
PspPI	GGNCC	6 cut(s)	149, 527, 946, 1003, 1004, 1165
RsaI	GTAC	1 cut(s)	802
RsaNI	GTAC	1 cut(s)	801
RseI	CAYNNNNRTG	3 cut(s)	701, 828, 852
SacI	GAGCTC	1 cut(s)	1194
SaqAI	TTAA	2 cut(s)	699, 780
SatI	GCNGC	5 cut(s)	50, 110, 402, 916, 1157
Sau3AI	GATC	4 cut(s)	153, 580, 820, 1185
Sau96I	GGNCC	6 cut(s)	149, 527, 946, 1003, 1004, 1165
SchI	GAGTC	1 cut(s)	623
ScrFI	CCNGG	3 cut(s)	116, 1135, 1220
SduI	GDGCHC	5 cut(s)	859, 1007, 1072, 1194, 1206
SfaNI	GCATC	2 cut(s)	264, 985
SfcI	CTRYAG	1 cut(s)	618
SinI	GGWCC	2 cut(s)	527, 1165
SmiMI	CAYNNNNRTG	3 cut(s)	701, 828, 852
SmlI	CTYRAG	1 cut(s)	1232
SmoI	CTYRAG	1 cut(s)	1232
Sse9I	AATT	7 cut(s)	23, 160, 285, 369, 414, 478, 886
SsiI	CCGC	3 cut(s)	49, 129, 376
SspI	AATATT	2 cut(s)	673, 1055
SspMI	CTAG	5 cut(s)	531, 1076, 1101, 1239, 1269
SstI	GAGCTC	1 cut(s)	1194
StyD4I	CCNGG	3 cut(s)	114, 1133, 1218
StyI	CCWWGG	1 cut(s)	215
TaaI	ACNGT	2 cut(s)	1040, 1091
TaiI	ACGT	3 cut(s)	176, 249, 730
TaqI	TCGA	4 cut(s)	67, 199, 277, 1188
TasI	AATT	7 cut(s)	23, 160, 285, 369, 414, 478, 886
TatI	WGTACW	1 cut(s)	800
TauI	GCSGC	1 cut(s)	52
TfiI	GAWTC	1 cut(s)	757
Tru1I	TTAA	2 cut(s)	699, 780
Tru9I	TTAA	2 cut(s)	699, 780
TseI	GCWGC	4 cut(s)	109, 401, 915, 1156
TspDTI	ATGAA	3 cut(s)	558, 638, 978
TspGWI	ACGGA	1 cut(s)	490
VneI	GTGCAC	1 cut(s)	855
VpaK11BI	GGWCC	2 cut(s)	527, 1165
XapI	RAATTY	2 cut(s)	23, 285
XbaI	TCTAGA	1 cut(s)	1100
XceI	RCATGY	1 cut(s)	1163
XmnI	GAANNNNTTC	1 cut(s)	205
XspI	CTAG	5 cut(s)	531, 1076, 1101, 1239, 1269
Zsp2I	ATGCAT	1 cut(s)	633

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.