RchiOBHm_Chr3g0467621

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0002302
Physical Location & Seq
Forward (+)
13729868 .. 13733990
4123 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ43357

Sequence Viewer

Length: 1302 bp
ATGGAGACGCAGAGGATAATTGAATTTCCTCACCAGAATATGGATAAGCGGCCCAGGAAGAGGCCTCGATTAACATGGGATATGCCCCCTCCTCTTCCTCCTCCCGAGCTGCTGCCAGCAATATACTGCGGGCCGGAGTTTGGAAGTGGGCCAATTCCCAATTACACATATACGTCTATGTTTTATGGGGGATTGCCTCGGAATGCCTCCCCTCCGTGGAGGCCTGATGATAAAGATGGTCATTATGTGTTTGGGATTGGAGATAATCTAACCCCTCGATATAGGATTCTCAACAAAATGGGAGAGGGGACTTTTGGGCAAGTGTTGGAGTGTTTTGACAATGAAAAGAAAGAATTTGTGGCAATTAAAATTATCCGCTCCATACATAAGTATCGTGAAGCTGCGATGATTGAAATTGATGTCCTACAAAGGCTTGCAAGGCATGATATTGGTGGCACTCGTTGTGTGCAAATTCGGAATTGGTTTGACTATCGTAATCATATTTGTATTGTATTTGAGAAGCTTGGACCTAGCTTATACGATTTTCTTCGCAAAAACAGCTACCGTTCATTTCCCATTGATCTTGTTCGGGAGCTTGCCAGACAACTTTTGGAGTCTGTAGCATTTATGCATGATTTACGCCTTATTCATACTGATTTGAAGCCGGAAAATATTCTGCTTGTTTCCTCCGACTACATCAAAGTGCCAGACTATAGGTTTCTGGCACGGTCAACTAAAGAGGGTTCTTATTTCAAGAATCTGCCCAAGTCAAGTGCCATAAAGCTCATTGATTTTGGAAGCACAACATTTGAACATCAGGATCATAGCTATGTGGTGTCAACACGCCATTATCGTGCACCGGAGGTTATCTTAGGTCTTGGGTGGAACTATCCTTGTGACATATGGAGTGTGGGCTGCATACTTGTTGAACTATGCTCTGGTGAGGCCCTTTTTCAAACACATGAGAACTTGGAGCATCTTGCCATGATGGAGAGGGTTTTGGGGCCACTGCCGCAGCATATGGTGCAGAGAGCTGACCGTCGTGCTGAGAAATATTTTAGGAGGGGAGCACGACTGGATTGGCCTGATGGGGCCGCATCTAGGGAAAGCATGAGAGCAGTATTCAAATTGCCCCGATTACCGAACTTAATAATGCAACATGTGGATCACTCTGCTGGTGATCTGATTGAACTCCTGCAAGGGCTCCTAAGATACGAGCCCGCAGAGCGGCTCAAGGCAAGGGAAGCACTAAGACACCCCTTTTTCACTAGAGATGTGAGAAGGGGTGGCTATCCCCTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0006355 GO:0008150 GO:0009889 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:1903506 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

433

Amino Acids

50.39

Weight (kDa)

9.0

Isoelectric Point (pI)

46.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 94 - 422 1.6e-48 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 96 - 331 7.7e-19 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 40
AccBSI CCGCTC 2 cut(s) 378, 1228
AciI CCGC 7 cut(s) 49, 129, 376, 1013, 1095, 1221, 1228
AclWI GGATC 2 cut(s) 828, 1173
AcsI RAATTY 3 cut(s) 23, 353, 471
AfiI CCNNNNNNNGG 6 cut(s) 40, 60, 140, 216, 1101, 1227
AflIII ACRYGT 1 cut(s) 1159
AgsI TTSAA 9 cut(s) 23, 413, 661, 754, 812, 929, 956, 1126, 1190
AjnI CCWGG 1 cut(s) 53
AluBI AGCT 9 cut(s) 109, 401, 523, 534, 561, 595, 784, 828, 1034
AluI AGCT 9 cut(s) 109, 401, 523, 534, 561, 595, 784, 828, 1034
Alw21I GWGCWC 2 cut(s) 859, 1072
Alw44I GTGCAC 1 cut(s) 855
AlwI GGATC 2 cut(s) 828, 1173
Ama87I CYCGRG 1 cut(s) 104
AoxI GGCC 9 cut(s) 50, 62, 131, 149, 221, 945, 1004, 1082, 1092
ApaLI GTGCAC 1 cut(s) 855
ApeKI GCWGC 5 cut(s) 109, 112, 401, 915, 1015
ApoI RAATTY 3 cut(s) 23, 353, 471
Asp700I GAANNNNTTC 1 cut(s) 672
AspS9I GGNCC 7 cut(s) 51, 131, 149, 527, 946, 1004, 1092
AsuHPI GGTGA 3 cut(s) 23, 953, 1190
AvaI CYCGRG 1 cut(s) 104
AvaII GGWCC 1 cut(s) 527
BaeGI GKGCMC 1 cut(s) 859
BaeI ACNNNNGTAYC 2 cut(s) 374, 407
BanII GRGCYC 2 cut(s) 1206, 1221
Bbv12I GWGCWC 2 cut(s) 859, 1072
BbvI GCAGC 5 cut(s) 96, 99, 388, 902, 1027
BccI CCATC 3 cut(s) 230, 982, 1082
BciT130I CCWGG 1 cut(s) 55
BfaI CTAG 3 cut(s) 531, 1101, 1269
BfmI CTRYAG 2 cut(s) 618, 712
BisI GCNGC 9 cut(s) 50, 110, 113, 402, 916, 1013, 1016, 1095, 1229
BlsI GCNGC 9 cut(s) 51, 111, 114, 403, 917, 1014, 1017, 1096, 1230
Bme1390I CCNGG 1 cut(s) 55
Bme18I GGWCC 1 cut(s) 527
BmeT110I CYCGRG 1 cut(s) 104
BmgT120I GGNCC 7 cut(s) 51, 131, 149, 527, 946, 1004, 1092
BmiI GGNNCC 3 cut(s) 1005, 1093, 1205
BmrFI CCNGG 1 cut(s) 55
BmsI GCATC 2 cut(s) 985, 1106
BpuEI CTTGAG 1 cut(s) 1217
BsaJI CCNNGG 3 cut(s) 53, 197, 215
BsaWI WCCGGW 1 cut(s) 859
Bsc4I CCNNNNNNNGG 6 cut(s) 40, 60, 140, 216, 1101, 1227
Bse1I ACTGG 1 cut(s) 1080
BseBI CCWGG 1 cut(s) 55
BseDI CCNNGG 3 cut(s) 53, 197, 215
BseLI CCNNNNNNNGG 6 cut(s) 40, 60, 140, 216, 1101, 1227
BseMII CTCAG 1 cut(s) 1038
BseNI ACTGG 1 cut(s) 1080
BseRI GAGGAG 2 cut(s) 81, 90
BseSI GKGCMC 1 cut(s) 859
BseXI GCAGC 5 cut(s) 96, 99, 388, 902, 1027
BsgI GTGCAG 1 cut(s) 1046
BshFI GGCC 9 cut(s) 52, 64, 133, 151, 223, 947, 1006, 1084, 1094
BsiHKAI GWGCWC 2 cut(s) 859, 1072
BsiHKCI CYCGRG 1 cut(s) 104
BsiSI CCGG 3 cut(s) 134, 665, 860
BslFI GGGAC 1 cut(s) 322
BslI CCNNNNNNNGG 6 cut(s) 40, 60, 140, 216, 1101, 1227
BsmFI GGGAC 1 cut(s) 322
BsmI GAATGC 1 cut(s) 208
BsnI GGCC 9 cut(s) 52, 64, 133, 151, 223, 947, 1006, 1084, 1094
BsoBI CYCGRG 1 cut(s) 104
Bsp1286I GDGCHC 4 cut(s) 859, 1072, 1206, 1221
Bsp143I GATC 4 cut(s) 580, 820, 1165, 1180
BspACI CCGC 7 cut(s) 49, 129, 376, 1013, 1095, 1221, 1228
BspANI GGCC 9 cut(s) 52, 64, 133, 151, 223, 947, 1006, 1084, 1094
BspCNI CTCAG 1 cut(s) 1039
BspLI GGNNCC 3 cut(s) 1005, 1093, 1205
BspPI GGATC 2 cut(s) 828, 1173
BsrBI CCGCTC 2 cut(s) 378, 1228
BsrI ACTGG 1 cut(s) 1080
BssECI CCNNGG 3 cut(s) 53, 197, 215
BssMI GATC 4 cut(s) 580, 820, 1165, 1180
Bst2UI CCWGG 1 cut(s) 55
Bst4CI ACNGT 3 cut(s) 566, 729, 1040
Bst6I CTCTTC 2 cut(s) 53, 99
BstAPI GCANNNNNTGC 1 cut(s) 1024
BstC8I GCNNGC 5 cut(s) 117, 131, 435, 597, 1221
BstDEI CTNAG 4 cut(s) 871, 1047, 1208, 1250
BstDSI CCRYGG 1 cut(s) 215
BstKTI GATC 4 cut(s) 583, 823, 1168, 1183
BstMBI GATC 4 cut(s) 580, 820, 1165, 1180
BstMWI GCNNNNNNNGC 6 cut(s) 439, 558, 1012, 1024, 1225, 1244
BstNI CCWGG 1 cut(s) 55
BstNSI RCATGY 1 cut(s) 1163
BstSCI CCNGG 1 cut(s) 53
BstSFI CTRYAG 2 cut(s) 618, 712
BstSLI GKGCMC 1 cut(s) 859
BstV1I GCAGC 5 cut(s) 96, 99, 388, 902, 1027
BsuRI GGCC 9 cut(s) 52, 64, 133, 151, 223, 947, 1006, 1084, 1094
BtgI CCRYGG 1 cut(s) 215
BtgZI GCGATG 1 cut(s) 419
BtsI GCAGTG 1 cut(s) 1007
BtsIMutI CAGTG 1 cut(s) 1007
Cac8I GCNNGC 5 cut(s) 117, 131, 435, 597, 1221
Cfr13I GGNCC 7 cut(s) 51, 131, 149, 527, 946, 1004, 1092
CseI GACGC 1 cut(s) 16
CviAII CATG 7 cut(s) 75, 443, 632, 962, 985, 1111, 1160
DdeI CTNAG 4 cut(s) 871, 1047, 1208, 1250
DpnI GATC 4 cut(s) 582, 822, 1167, 1182
DpnII GATC 4 cut(s) 580, 820, 1165, 1180
Eam1104I CTCTTC 2 cut(s) 53, 99
EarI CTCTTC 2 cut(s) 53, 99
Eco147I AGGCCT 2 cut(s) 64, 223
Eco24I GRGCYC 2 cut(s) 1206, 1221
Eco47I GGWCC 1 cut(s) 527
Eco88I CYCGRG 1 cut(s) 104
EcoO109I RGGNCCY 1 cut(s) 946
EcoRII CCWGG 1 cut(s) 53
EcoT22I ATGCAT 1 cut(s) 633
EcoT38I GRGCYC 2 cut(s) 1206, 1221
FaeI CATG 7 cut(s) 78, 446, 635, 965, 988, 1114, 1163
FaqI GGGAC 1 cut(s) 322
FatI CATG 7 cut(s) 74, 442, 631, 961, 984, 1110, 1159
FauI CCCGC 2 cut(s) 122, 1228
FauNDI CATATG 2 cut(s) 902, 1020
Fnu4HI GCNGC 9 cut(s) 50, 110, 113, 402, 916, 1013, 1016, 1095, 1229
FriOI GRGCYC 2 cut(s) 1206, 1221
Fsp4HI GCNGC 9 cut(s) 50, 110, 113, 402, 916, 1013, 1016, 1095, 1229
FspBI CTAG 3 cut(s) 531, 1101, 1269
GluI GCNGC 9 cut(s) 50, 110, 113, 402, 916, 1013, 1016, 1095, 1229
HaeIII GGCC 9 cut(s) 52, 64, 133, 151, 223, 947, 1006, 1084, 1094
HapII CCGG 3 cut(s) 134, 665, 860
HgaI GACGC 1 cut(s) 16
Hin1II CATG 7 cut(s) 78, 446, 635, 965, 988, 1114, 1163
HincII GTYRAC 2 cut(s) 732, 840
HindII GTYRAC 2 cut(s) 732, 840
HindIII AAGCTT 1 cut(s) 521
HinfI GANTC 3 cut(s) 286, 614, 757
HpaII CCGG 3 cut(s) 134, 665, 860
HphI GGTGA 3 cut(s) 23, 953, 1190
Hpy166II GTNNAC 3 cut(s) 732, 840, 857
Hpy188I TCNGA 4 cut(s) 201, 477, 691, 1185
Hpy188III TCNNGA 5 cut(s) 104, 395, 590, 754, 818
Hpy8I GTNNAC 3 cut(s) 732, 840, 857
Hpy99I CGWCG 1 cut(s) 1044
HpyAV CCTTC 1 cut(s) 1275
HpyCH4III ACNGT 3 cut(s) 566, 729, 1040
HpyCH4IV ACGT 1 cut(s) 173
HpyCH4V TGCA 8 cut(s) 437, 469, 631, 857, 918, 1027, 1156, 1198
HpyF10VI GCNNNNNNNGC 6 cut(s) 439, 558, 1012, 1024, 1225, 1244
HpyF3I CTNAG 4 cut(s) 871, 1047, 1208, 1250
HpySE526I ACGT 1 cut(s) 173
Hsp92II CATG 7 cut(s) 78, 446, 635, 965, 988, 1114, 1163
Kzo9I GATC 4 cut(s) 580, 820, 1165, 1180
LmnI GCTCC 5 cut(s) 383, 592, 973, 1067, 1209
Lsp1109I GCAGC 5 cut(s) 96, 99, 388, 902, 1027
LweI GCATC 2 cut(s) 985, 1106
MaeI CTAG 3 cut(s) 531, 1101, 1269
MaeII ACGT 1 cut(s) 173
MaeIII GTNAC 1 cut(s) 896
MalI GATC 4 cut(s) 582, 822, 1167, 1182
MbiI CCGCTC 2 cut(s) 378, 1228
MboI GATC 4 cut(s) 580, 820, 1165, 1180
MboII GAAGA 3 cut(s) 70, 86, 539
MhlI GDGCHC 4 cut(s) 859, 1072, 1206, 1221
MlyI GAGTC 1 cut(s) 623
MmeI TCCRAC 2 cut(s) 306, 714
Mph1103I ATGCAT 1 cut(s) 633
MroXI GAANNNNTTC 1 cut(s) 672
MseI TTAA 3 cut(s) 71, 366, 1148
MslI CAYNNNNRTG 3 cut(s) 701, 828, 852
MspI CCGG 3 cut(s) 134, 665, 860
MspR9I CCNGG 1 cut(s) 55
Mva1269I GAATGC 1 cut(s) 208
MvaI CCWGG 1 cut(s) 55
MwoI GCNNNNNNNGC 6 cut(s) 439, 558, 1012, 1024, 1225, 1244
NdeI CATATG 2 cut(s) 902, 1020
NdeII GATC 4 cut(s) 580, 820, 1165, 1180
NlaIII CATG 7 cut(s) 78, 446, 635, 965, 988, 1114, 1163
NlaIV GGNNCC 3 cut(s) 1005, 1093, 1205
NmuCI GTSAC 1 cut(s) 896
NsiI ATGCAT 1 cut(s) 633
NspI RCATGY 1 cut(s) 1163
PceI AGGCCT 2 cut(s) 64, 223
PciI ACATGT 1 cut(s) 1159
PcsI WCGNNNNNNNCGW 1 cut(s) 850
PctI GAATGC 1 cut(s) 208
PdmI GAANNNNTTC 1 cut(s) 672
PfeI GAWTC 2 cut(s) 286, 757
PflMI CCANNNNNTGG 1 cut(s) 40
PkrI GCNGC 9 cut(s) 51, 111, 114, 403, 917, 1014, 1017, 1096, 1230
PleI GAGTC 1 cut(s) 622
PpsI GAGTC 1 cut(s) 622
PscI ACATGT 1 cut(s) 1159
Psp6I CCWGG 1 cut(s) 53
PspGI CCWGG 1 cut(s) 53
PspN4I GGNNCC 3 cut(s) 1005, 1093, 1205
PspPI GGNCC 7 cut(s) 51, 131, 149, 527, 946, 1004, 1092
RseI CAYNNNNRTG 3 cut(s) 701, 828, 852
SaqAI TTAA 3 cut(s) 71, 366, 1148
SatI GCNGC 9 cut(s) 50, 110, 113, 402, 916, 1013, 1016, 1095, 1229
Sau3AI GATC 4 cut(s) 580, 820, 1165, 1180
Sau96I GGNCC 7 cut(s) 51, 131, 149, 527, 946, 1004, 1092
SchI GAGTC 1 cut(s) 623
ScrFI CCNGG 1 cut(s) 55
SduI GDGCHC 4 cut(s) 859, 1072, 1206, 1221
SfaNI GCATC 2 cut(s) 985, 1106
SfcI CTRYAG 2 cut(s) 618, 712
SinI GGWCC 1 cut(s) 527
SmiMI CAYNNNNRTG 3 cut(s) 701, 828, 852
SmlI CTYRAG 1 cut(s) 1232
SmoI CTYRAG 1 cut(s) 1232
SseBI AGGCCT 2 cut(s) 64, 223
SsiI CCGC 7 cut(s) 49, 129, 376, 1013, 1095, 1221, 1228
SspI AATATT 2 cut(s) 673, 1055
SspMI CTAG 3 cut(s) 531, 1101, 1269
StuI AGGCCT 2 cut(s) 64, 223
StyD4I CCNGG 1 cut(s) 53
TaaI ACNGT 3 cut(s) 566, 729, 1040
TaiI ACGT 1 cut(s) 176
TaqI TCGA 2 cut(s) 67, 277
TauI GCSGC 4 cut(s) 52, 1015, 1097, 1231
TfiI GAWTC 2 cut(s) 286, 757
Tru1I TTAA 3 cut(s) 71, 366, 1148
Tru9I TTAA 3 cut(s) 71, 366, 1148
TscAI CASTG 1 cut(s) 1014
TseFI GTSAC 1 cut(s) 896
TseI GCWGC 5 cut(s) 109, 112, 401, 915, 1015
Tsp45I GTSAC 1 cut(s) 896
TspDTI ATGAA 3 cut(s) 357, 558, 638
TspGWI ACGGA 1 cut(s) 204
TspRI CASTG 1 cut(s) 1014
Van91I CCANNNNNTGG 1 cut(s) 40
VneI GTGCAC 1 cut(s) 855
VpaK11BI GGWCC 1 cut(s) 527
XapI RAATTY 3 cut(s) 23, 353, 471
XceI RCATGY 1 cut(s) 1163
XcmI CCANNNNNNNNNTGG 1 cut(s) 607
XmnI GAANNNNTTC 1 cut(s) 672
XspI CTAG 3 cut(s) 531, 1101, 1269
Zsp2I ATGCAT 1 cut(s) 633
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.