FvH4_6g36141

GPN-loop GTPase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0002226
Physical Location & Seq
Reverse (-)
28421436 .. 28424228
2793 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g36141.t1

Sequence Viewer

Length: 612 bp
ATGGAGTTTGTCTCTGTGGGGATCCTTTCCACTCTGACCCAACAAGGCTGGCCTTTGCTGTTGACCCACAACAGCTCTATTGACAGGAGGTCTAGTCTCCATGTTCAGGTGTTTAGAGCAGTTTGGTTGAGGACGCCGCAGTGTACCATTATAGGCAGCATTGACAAGCCAGAAGACAGGGCGTTTTTGAAGCAGCCTAGCAAAAGCAAAGGAAACAAACCCGGATATCCGAAAATGGGTTATGCACAGTTAGTTATTGGCCCTGCTGGCAGTGGCAAGGATTGGTGTTTTCACCGACACTGTTTAGATAATGGAAGGACTCTACATGTTGTTAACTTGGATTCTGCTGCGGAGCATTTCGACTACCCTGTAGCTTTGGATATCAGGGAGCTTATTAACTTGGATGATGTTATGGAGGAACTTGGACTGGGTCCCAATGGTGGACTTATGTACTGCATGGAACACCTTGAAGATAATCTGGATGATTGGCTTGCAGAGGGATTGGAGGAGTACTTGGATGATGACTACTTAGTTTTTGACTGCCCAGGCCAGATAGAACTTTTTTCTCATGTGCCTATGCTTCGGAACTTTGTGGACCATTTAAAGAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ATP_bind_1

Protein Analysis

204

Amino Acids

22.97

Weight (kDa)

4.96

Isoelectric Point (pI)

43.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ATP_bind_1 PF03029 85 - 203 4.4e-41 Conserved hypothetical ATP binding protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 137, 350
AclWI GGATC 2 cut(s) 16, 29
AcyI GRCGYC 1 cut(s) 134
AfaI GTAC 3 cut(s) 145, 452, 512
AfiI CCNNNNNNNGG 3 cut(s) 106, 236, 440
AflIII ACRYGT 1 cut(s) 325
AgsI TTSAA 2 cut(s) 190, 470
AhdI GACNNNNNGTC 1 cut(s) 88
AjnI CCWGG 1 cut(s) 544
AluBI AGCT 4 cut(s) 75, 374, 391, 609
AluI AGCT 4 cut(s) 75, 374, 391, 609
Alw26I GTCTC 2 cut(s) 16, 101
AlwI GGATC 2 cut(s) 16, 29
AoxI GGCC 3 cut(s) 50, 259, 547
ApeKI GCWGC 3 cut(s) 156, 193, 347
AspS9I GGNCC 3 cut(s) 260, 431, 595
AsuC2I CCSGG 1 cut(s) 222
AsuHPI GGTGA 1 cut(s) 284
AvaII GGWCC 2 cut(s) 431, 595
BamHI GGATCC 1 cut(s) 21
BbsI GAAGAC 1 cut(s) 180
BbvI GCAGC 3 cut(s) 168, 205, 334
BciT130I CCWGG 1 cut(s) 546
BcnI CCSGG 1 cut(s) 222
BcoDI GTCTC 2 cut(s) 16, 101
BfaI CTAG 3 cut(s) 93, 198, 610
BfmI CTRYAG 1 cut(s) 369
BglI GCCNNNNNGGC 1 cut(s) 267
BisI GCNGC 4 cut(s) 137, 157, 194, 348
BlsI GCNGC 4 cut(s) 138, 158, 195, 349
BmcAI AGTACT 1 cut(s) 512
Bme1390I CCNGG 2 cut(s) 222, 546
Bme18I GGWCC 2 cut(s) 431, 595
BmeRI GACNNNNNGTC 1 cut(s) 88
BmgT120I GGNCC 3 cut(s) 260, 431, 595
BmiI GGNNCC 3 cut(s) 23, 432, 433
BmrFI CCNGG 2 cut(s) 222, 546
BmrI ACTGGG 1 cut(s) 437
BmuI ACTGGG 1 cut(s) 437
BpiI GAAGAC 1 cut(s) 180
BplI GAGNNNNNCTC 1 cut(s) 28
BpuMI CCSGG 1 cut(s) 222
BsaHI GRCGYC 1 cut(s) 134
BsaJI CCNNGG 1 cut(s) 544
BsaXI ACNNNNNCTCC 2 cut(s) 79, 109
Bsc4I CCNNNNNNNGG 3 cut(s) 106, 236, 440
Bse1I ACTGG 1 cut(s) 432
BseBI CCWGG 1 cut(s) 546
BseDI CCNNGG 1 cut(s) 544
BseGI GGATG 3 cut(s) 409, 487, 523
BseLI CCNNNNNNNGG 3 cut(s) 106, 236, 440
BseNI ACTGG 1 cut(s) 432
BseRI GAGGAG 1 cut(s) 521
BseXI GCAGC 3 cut(s) 168, 205, 334
BshFI GGCC 3 cut(s) 52, 261, 549
BsiSI CCGG 1 cut(s) 222
BslFI GGGAC 1 cut(s) 417
BslI CCNNNNNNNGG 3 cut(s) 106, 236, 440
BsmAI GTCTC 2 cut(s) 16, 101
BsmFI GGGAC 1 cut(s) 417
BsnI GGCC 3 cut(s) 52, 261, 549
Bsp143I GATC 1 cut(s) 21
BspACI CCGC 2 cut(s) 137, 350
BspANI GGCC 3 cut(s) 52, 261, 549
BspLI GGNNCC 3 cut(s) 23, 432, 433
BspPI GGATC 2 cut(s) 16, 29
BsrI ACTGG 1 cut(s) 432
BssECI CCNNGG 1 cut(s) 544
BssMI GATC 1 cut(s) 21
BssNI GRCGYC 1 cut(s) 134
Bst2UI CCWGG 1 cut(s) 546
Bst4CI ACNGT 2 cut(s) 249, 302
BstACI GRCGYC 1 cut(s) 134
BstC8I GCNNGC 3 cut(s) 50, 268, 492
BstDEI CTNAG 1 cut(s) 529
BstF5I GGATG 3 cut(s) 409, 487, 523
BstKTI GATC 1 cut(s) 24
BstMAI GTCTC 2 cut(s) 16, 101
BstMBI GATC 1 cut(s) 21
BstMWI GCNNNNNNNGC 1 cut(s) 267
BstNI CCWGG 1 cut(s) 546
BstNSI RCATGY 1 cut(s) 329
BstSCI CCNGG 2 cut(s) 220, 544
BstSFI CTRYAG 1 cut(s) 369
BstV1I GCAGC 3 cut(s) 168, 205, 334
BstV2I GAAGAC 1 cut(s) 180
BstX2I RGATCY 1 cut(s) 21
BstYI RGATCY 1 cut(s) 21
BsuRI GGCC 3 cut(s) 52, 261, 549
BtsCI GGATG 3 cut(s) 409, 487, 523
BtsI GCAGTG 2 cut(s) 146, 277
BtsIMutI CAGTG 3 cut(s) 146, 277, 298
Cac8I GCNNGC 3 cut(s) 50, 268, 492
Cfr13I GGNCC 3 cut(s) 260, 431, 595
CseI GACGC 1 cut(s) 142
Csp6I GTAC 3 cut(s) 144, 451, 511
CviAII CATG 4 cut(s) 101, 326, 457, 569
CviQI GTAC 3 cut(s) 144, 451, 511
DdeI CTNAG 1 cut(s) 529
DpnI GATC 1 cut(s) 23
DpnII GATC 1 cut(s) 21
DraI TTTAAA 1 cut(s) 603
DriI GACNNNNNGTC 1 cut(s) 88
Eam1105I GACNNNNNGTC 1 cut(s) 88
Eco32I GATATC 2 cut(s) 227, 382
Eco47I GGWCC 2 cut(s) 431, 595
EcoO109I RGGNCCY 1 cut(s) 431
EcoRII CCWGG 1 cut(s) 544
EcoRV GATATC 2 cut(s) 227, 382
FaeI CATG 4 cut(s) 104, 329, 460, 572
FaiI YATR 9 cut(s) 102, 152, 243, 327, 413, 449, 458, 570, 578
FaqI GGGAC 1 cut(s) 417
FatI CATG 4 cut(s) 100, 325, 456, 568
Fnu4HI GCNGC 4 cut(s) 137, 157, 194, 348
FokI GGATG 3 cut(s) 416, 494, 530
Fsp4HI GCNGC 4 cut(s) 137, 157, 194, 348
FspBI CTAG 3 cut(s) 93, 198, 610
GluI GCNGC 4 cut(s) 137, 157, 194, 348
HaeIII GGCC 3 cut(s) 52, 261, 549
HapII CCGG 1 cut(s) 222
HgaI GACGC 1 cut(s) 142
Hin1I GRCGYC 1 cut(s) 134
Hin1II CATG 4 cut(s) 104, 329, 460, 572
HincII GTYRAC 2 cut(s) 63, 334
HindII GTYRAC 2 cut(s) 63, 334
HinfI GANTC 2 cut(s) 319, 341
HpaI GTTAAC 1 cut(s) 334
HpaII CCGG 1 cut(s) 222
HphI GGTGA 1 cut(s) 284
Hpy166II GTNNAC 5 cut(s) 63, 144, 334, 443, 595
Hpy188I TCNGA 3 cut(s) 36, 231, 585
Hpy188III TCNNGA 1 cut(s) 479
Hpy8I GTNNAC 5 cut(s) 63, 144, 334, 443, 595
HpyAV CCTTC 1 cut(s) 309
HpyCH4III ACNGT 2 cut(s) 249, 302
HpyCH4V TGCA 3 cut(s) 245, 456, 494
HpyF10VI GCNNNNNNNGC 1 cut(s) 267
HpyF3I CTNAG 1 cut(s) 529
Hsp92I GRCGYC 1 cut(s) 134
Hsp92II CATG 4 cut(s) 104, 329, 460, 572
KflI GGGWCCC 1 cut(s) 431
KspAI GTTAAC 1 cut(s) 334
Kzo9I GATC 1 cut(s) 21
LmnI GCTCC 2 cut(s) 352, 388
Lsp1109I GCAGC 3 cut(s) 168, 205, 334
MaeI CTAG 3 cut(s) 93, 198, 610
MalI GATC 1 cut(s) 23
MboI GATC 1 cut(s) 21
MboII GAAGA 2 cut(s) 185, 482
MflI RGATCY 1 cut(s) 21
MlyI GAGTC 1 cut(s) 313
MnlI CCTC 5 cut(s) 81, 123, 409, 490, 499
MseI TTAA 3 cut(s) 333, 396, 602
MspI CCGG 1 cut(s) 222
MspR9I CCNGG 2 cut(s) 222, 546
MvaI CCWGG 1 cut(s) 546
MwoI GCNNNNNNNGC 1 cut(s) 267
NciI CCSGG 1 cut(s) 222
NdeII GATC 1 cut(s) 21
NlaIII CATG 4 cut(s) 104, 329, 460, 572
NlaIV GGNNCC 3 cut(s) 23, 432, 433
NspI RCATGY 1 cut(s) 329
PciI ACATGT 1 cut(s) 325
PfeI GAWTC 1 cut(s) 341
PflFI GACNNNGTC 1 cut(s) 429
PkrI GCNGC 4 cut(s) 138, 158, 195, 349
PleI GAGTC 1 cut(s) 313
PpsI GAGTC 1 cut(s) 313
PpuMI RGGWCCY 1 cut(s) 431
PscI ACATGT 1 cut(s) 325
Psp5II RGGWCCY 1 cut(s) 431
Psp6I CCWGG 1 cut(s) 544
PspGI CCWGG 1 cut(s) 544
PspN4I GGNNCC 3 cut(s) 23, 432, 433
PspPI GGNCC 3 cut(s) 260, 431, 595
PspPPI RGGWCCY 1 cut(s) 431
PsuI RGATCY 1 cut(s) 21
PsyI GACNNNGTC 1 cut(s) 429
RsaI GTAC 3 cut(s) 145, 452, 512
RsaNI GTAC 3 cut(s) 144, 451, 511
SaqAI TTAA 3 cut(s) 333, 396, 602
SatI GCNGC 4 cut(s) 137, 157, 194, 348
Sau3AI GATC 1 cut(s) 21
Sau96I GGNCC 3 cut(s) 260, 431, 595
ScaI AGTACT 1 cut(s) 512
SchI GAGTC 1 cut(s) 313
ScrFI CCNGG 2 cut(s) 222, 546
SetI ASST 7 cut(s) 77, 92, 111, 376, 393, 468, 611
SfcI CTRYAG 1 cut(s) 369
SinI GGWCC 2 cut(s) 431, 595
SsiI CCGC 2 cut(s) 137, 350
SspMI CTAG 3 cut(s) 93, 198, 610
StyD4I CCNGG 2 cut(s) 220, 544
TaaI ACNGT 2 cut(s) 249, 302
TaqI TCGA 1 cut(s) 360
TatI WGTACW 2 cut(s) 450, 510
TauI GCSGC 1 cut(s) 139
TfiI GAWTC 1 cut(s) 341
Tru1I TTAA 3 cut(s) 333, 396, 602
Tru9I TTAA 3 cut(s) 333, 396, 602
TscAI CASTG 3 cut(s) 146, 277, 305
TseI GCWGC 3 cut(s) 156, 193, 347
TspRI CASTG 3 cut(s) 146, 277, 305
Tth111I GACNNNGTC 1 cut(s) 429
VpaK11BI GGWCC 2 cut(s) 431, 595
XceI RCATGY 1 cut(s) 329
XspI CTAG 3 cut(s) 93, 198, 610
ZrmI AGTACT 1 cut(s) 512
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.