MD17G1145200.v1.1

GPN-loop GTPase 3 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0002226
Physical Location & Seq
Forward (+)
13104792 .. 13109283
4492 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1145200.v1.1.491

Sequence Viewer

Length: 804 bp
ATGGGTTACGCGCAGCTGGTTATTGGCCCTGCCGGCAGCGGCAAGTCGACTTATTGCTCTAGCTTGCACGAACACTGTACGGCTATTGGGAGGAATATCAATATTGTTAATCTGGATCCTGCTGCTGAAAATTTTGACTACCCTGTGGCCATGGATATTAGGGAGCTTGTTTCCTTGGAGGATGTTATGGAGGAACTTGGTTTGGGTCCCAATGGTGGACTTTTATACTGCATGGAACACCTTGAGGACAATCTGGATGAATGGCTCACAGAGGAATTAGACAATTACACGGATGATGACTACTTGGTTTTTGACTGCCCAGGCCAGATAGAACTTTTCTCGCATGTGCCTGTGCTTCGGAACTTCGTGGAGCATCTGCAGCGTAAAAATTTCAAAGTCTGCGTCGTGTACTTGCTTGATTCTCAGTTTATTACGGATGTGACAAAGTTTATTAGTGGGTGCATGGCATCTCTTTCTGCCATGATTCAACTTGAATTACCACATGTTAATATTCTCTCCAAAATGGACCTTGCACCGAGAAAAAAGGATATTGAAGACTTCTTGTATCCGGAGCCTCAAGTTTTATTGTCAGAGTTGAATCAACGCATGGCTCCTCAGTTTGCAAAGCTAAATAAATCTTTGATTGAACTAGTCGATGATTATAGCATGGTGAGTTTTGTGCCACTAGACTTGAGGAAGTCAAGCAGCATGAGCTATGTCTTGGCTCAGATCGATACCTGCATTCAGTTCGGAGAAGATGCCGATGTGAAGGTTAAGGAATTTGATCAGGAAGAAGGTGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ATP_bind_1

Protein Analysis

268

Amino Acids

30.18

Weight (kDa)

4.34

Isoelectric Point (pI)

47.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ATP_bind_1 PF03029 7 - 249 2e-84 Conserved hypothetical ATP binding protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 746
AccI GTMKAC 1 cut(s) 47
AccII CGCG 1 cut(s) 11
AccIII TCCGGA 1 cut(s) 568
AciI CCGC 1 cut(s) 39
AclWI GGATC 2 cut(s) 110, 123
AcoI YGGCCR 1 cut(s) 147
AcsI RAATTY 3 cut(s) 130, 388, 779
AfaI GTAC 2 cut(s) 79, 410
AfiI CCNNNNNNNGG 1 cut(s) 215
AflIII ACRYGT 1 cut(s) 502
AgsI TTSAA 6 cut(s) 394, 488, 494, 554, 598, 647
AhlI ACTAGT 1 cut(s) 649
AjnI CCWGG 1 cut(s) 319
AluBI AGCT 5 cut(s) 16, 63, 166, 628, 714
AluI AGCT 5 cut(s) 16, 63, 166, 628, 714
AlwI GGATC 2 cut(s) 110, 123
Aor13HI TCCGGA 1 cut(s) 568
AoxI GGCC 3 cut(s) 25, 147, 322
ApeKI GCWGC 5 cut(s) 13, 36, 122, 379, 705
ApoI RAATTY 3 cut(s) 130, 388, 779
ArsI GACNNNNNNTTYG 2 cut(s) 387, 419
AspLEI GCGC 1 cut(s) 13
AspS9I GGNCC 3 cut(s) 26, 206, 526
AsuHPI GGTGA 1 cut(s) 682
AvaII GGWCC 2 cut(s) 206, 526
BalI TGGCCA 1 cut(s) 149
BamHI GGATCC 1 cut(s) 115
BbsI GAAGAC 1 cut(s) 561
BbvI GCAGC 5 cut(s) 25, 48, 109, 391, 717
BceAI ACGGC 1 cut(s) 96
BcgI CGANNNNNNTGC 2 cut(s) 730, 764
BciT130I CCWGG 1 cut(s) 321
BciVI GTATCC 1 cut(s) 576
BclI TGATCA 1 cut(s) 784
BcuI ACTAGT 1 cut(s) 649
BfaI CTAG 3 cut(s) 60, 650, 686
BfmI CTRYAG 1 cut(s) 377
BfuAI ACCTGC 1 cut(s) 746
BfuI GTATCC 1 cut(s) 576
BglI GCCNNNNNGGC 1 cut(s) 33
BisI GCNGC 6 cut(s) 14, 37, 40, 123, 380, 706
BlsI GCNGC 6 cut(s) 15, 38, 41, 124, 381, 707
Bme1390I CCNGG 1 cut(s) 321
Bme18I GGWCC 2 cut(s) 206, 526
BmgT120I GGNCC 3 cut(s) 26, 206, 526
BmiI GGNNCC 5 cut(s) 117, 207, 208, 573, 612
BmrFI CCNGG 1 cut(s) 321
BmsI GCATC 3 cut(s) 382, 476, 748
BpiI GAAGAC 1 cut(s) 561
BpuEI CTTGAG 3 cut(s) 263, 561, 712
Bsa29I ATCGAT 1 cut(s) 732
BsaJI CCNNGG 3 cut(s) 150, 174, 319
BsaWI WCCGGW 1 cut(s) 568
Bsc4I CCNNNNNNNGG 1 cut(s) 215
Bse118I RCCGGY 1 cut(s) 32
BseAI TCCGGA 1 cut(s) 568
BseBI CCWGG 1 cut(s) 321
BseCI ATCGAT 1 cut(s) 732
BseDI CCNNGG 3 cut(s) 150, 174, 319
BseGI GGATG 4 cut(s) 187, 262, 298, 442
BseLI CCNNNNNNNGG 1 cut(s) 215
BseMII CTCAG 3 cut(s) 437, 629, 740
BseRI GAGGAG 1 cut(s) 603
BseXI GCAGC 5 cut(s) 25, 48, 109, 391, 717
Bsh1236I CGCG 1 cut(s) 11
BshFI GGCC 3 cut(s) 27, 149, 324
BshVI ATCGAT 1 cut(s) 732
BsiSI CCGG 2 cut(s) 33, 569
BslFI GGGAC 1 cut(s) 192
BslI CCNNNNNNNGG 1 cut(s) 215
BsmFI GGGAC 1 cut(s) 192
BsmI GAATGC 1 cut(s) 741
BsnI GGCC 3 cut(s) 27, 149, 324
Bsp13I TCCGGA 1 cut(s) 568
Bsp143I GATC 3 cut(s) 115, 729, 784
Bsp19I CCATGG 1 cut(s) 150
BspACI CCGC 1 cut(s) 39
BspANI GGCC 3 cut(s) 27, 149, 324
BspCNI CTCAG 3 cut(s) 436, 628, 739
BspDI ATCGAT 1 cut(s) 732
BspEI TCCGGA 1 cut(s) 568
BspFNI CGCG 1 cut(s) 11
BspLI GGNNCC 5 cut(s) 117, 207, 208, 573, 612
BspMAI CTGCAG 1 cut(s) 381
BspMI ACCTGC 1 cut(s) 746
BspPI GGATC 2 cut(s) 110, 123
BsrFI RCCGGY 1 cut(s) 32
BssAI RCCGGY 1 cut(s) 32
BssECI CCNNGG 3 cut(s) 150, 174, 319
BssMI GATC 3 cut(s) 115, 729, 784
BssT1I CCWWGG 2 cut(s) 150, 174
Bst2UI CCWGG 1 cut(s) 321
Bst4CI ACNGT 1 cut(s) 77
BstC8I GCNNGC 2 cut(s) 34, 65
BstDEI CTNAG 3 cut(s) 423, 615, 726
BstDSI CCRYGG 1 cut(s) 150
BstF5I GGATG 4 cut(s) 187, 262, 298, 442
BstFNI CGCG 1 cut(s) 11
BstHHI GCGC 1 cut(s) 13
BstKTI GATC 3 cut(s) 118, 732, 787
BstMBI GATC 3 cut(s) 115, 729, 784
BstMWI GCNNNNNNNGC 3 cut(s) 33, 379, 711
BstNI CCWGG 1 cut(s) 321
BstNSI RCATGY 2 cut(s) 347, 506
BstSCI CCNGG 1 cut(s) 319
BstSFI CTRYAG 1 cut(s) 377
BstUI CGCG 1 cut(s) 11
BstV1I GCAGC 5 cut(s) 25, 48, 109, 391, 717
BstV2I GAAGAC 1 cut(s) 561
BstX2I RGATCY 1 cut(s) 115
BstYI RGATCY 1 cut(s) 115
Bsu15I ATCGAT 1 cut(s) 732
BsuI GTATCC 1 cut(s) 576
BsuRI GGCC 3 cut(s) 27, 149, 324
BsuTUI ATCGAT 1 cut(s) 732
BtgI CCRYGG 1 cut(s) 150
BtsCI GGATG 4 cut(s) 187, 262, 298, 442
BtsIMutI CAGTG 1 cut(s) 73
BveI ACCTGC 1 cut(s) 746
Cac8I GCNNGC 2 cut(s) 34, 65
CfoI GCGC 1 cut(s) 13
Cfr10I RCCGGY 1 cut(s) 32
Cfr13I GGNCC 3 cut(s) 26, 206, 526
ClaI ATCGAT 1 cut(s) 732
CseI GACGC 1 cut(s) 391
Csp6I GTAC 2 cut(s) 78, 409
CspCI CAANNNNNGTGG 2 cut(s) 672, 707
CviAII CATG 9 cut(s) 151, 232, 344, 463, 481, 503, 607, 667, 709
CviQI GTAC 2 cut(s) 78, 409
DdeI CTNAG 3 cut(s) 423, 615, 726
DpnI GATC 3 cut(s) 117, 731, 786
DpnII GATC 3 cut(s) 115, 729, 784
EaeI YGGCCR 1 cut(s) 147
Eco130I CCWWGG 2 cut(s) 150, 174
Eco47I GGWCC 2 cut(s) 206, 526
EcoO109I RGGNCCY 1 cut(s) 206
EcoRII CCWGG 1 cut(s) 319
EcoT14I CCWWGG 2 cut(s) 150, 174
ErhI CCWWGG 2 cut(s) 150, 174
FaeI CATG 9 cut(s) 154, 235, 347, 466, 484, 506, 610, 670, 712
FaqI GGGAC 1 cut(s) 192
FatI CATG 9 cut(s) 150, 231, 343, 462, 480, 502, 606, 666, 708
FbaI TGATCA 1 cut(s) 784
FblI GTMKAC 1 cut(s) 47
Fnu4HI GCNGC 6 cut(s) 14, 37, 40, 123, 380, 706
FokI GGATG 4 cut(s) 194, 269, 305, 449
Fsp4HI GCNGC 6 cut(s) 14, 37, 40, 123, 380, 706
FspBI CTAG 3 cut(s) 60, 650, 686
GlaI GCGC 1 cut(s) 12
GluI GCNGC 6 cut(s) 14, 37, 40, 123, 380, 706
HaeIII GGCC 3 cut(s) 27, 149, 324
HapII CCGG 2 cut(s) 33, 569
HgaI GACGC 1 cut(s) 391
HhaI GCGC 1 cut(s) 13
Hin1II CATG 9 cut(s) 154, 235, 347, 466, 484, 506, 610, 670, 712
Hin6I GCGC 1 cut(s) 11
HinP1I GCGC 1 cut(s) 11
HincII GTYRAC 1 cut(s) 48
HindII GTYRAC 1 cut(s) 48
HinfI GANTC 3 cut(s) 419, 484, 598
HpaII CCGG 2 cut(s) 33, 569
HphI GGTGA 1 cut(s) 682
Hpy166II GTNNAC 3 cut(s) 48, 218, 409
Hpy188I TCNGA 4 cut(s) 360, 592, 729, 752
Hpy188III TCNNGA 4 cut(s) 113, 254, 569, 788
Hpy8I GTNNAC 3 cut(s) 48, 218, 409
Hpy99I CGWCG 1 cut(s) 407
HpyAV CCTTC 2 cut(s) 763, 788
HpyCH4III ACNGT 1 cut(s) 77
HpyCH4V TGCA 7 cut(s) 67, 231, 379, 462, 533, 623, 741
HpyF10VI GCNNNNNNNGC 3 cut(s) 33, 379, 711
HpyF3I CTNAG 3 cut(s) 423, 615, 726
Hsp92II CATG 9 cut(s) 154, 235, 347, 466, 484, 506, 610, 670, 712
HspAI GCGC 1 cut(s) 11
KflI GGGWCCC 1 cut(s) 206
Kpn2I TCCGGA 1 cut(s) 568
KroI GCCGGC 1 cut(s) 32
KroNI GCCGGC 1 cut(s) 34
Ksp22I TGATCA 1 cut(s) 784
Kzo9I GATC 3 cut(s) 115, 729, 784
LmnI GCTCC 4 cut(s) 163, 370, 571, 616
Lsp1109I GCAGC 5 cut(s) 25, 48, 109, 391, 717
LweI GCATC 3 cut(s) 382, 476, 748
MaeI CTAG 3 cut(s) 60, 650, 686
MaeIII GTNAC 3 cut(s) 5, 439, 797
MalI GATC 3 cut(s) 117, 731, 786
MboI GATC 3 cut(s) 115, 729, 784
MboII GAAGA 3 cut(s) 566, 767, 803
MflI RGATCY 1 cut(s) 115
MlsI TGGCCA 1 cut(s) 149
MluCI AATT 6 cut(s) 130, 275, 283, 388, 494, 779
MluNI TGGCCA 1 cut(s) 149
MnlI CCTC 8 cut(s) 84, 172, 184, 238, 265, 585, 624, 687
Mox20I TGGCCA 1 cut(s) 149
MroI TCCGGA 1 cut(s) 568
MroNI GCCGGC 1 cut(s) 32
MscI TGGCCA 1 cut(s) 149
MseI TTAA 3 cut(s) 108, 507, 774
Msp20I TGGCCA 1 cut(s) 149
MspA1I CMGCKG 2 cut(s) 16, 39
MspI CCGG 2 cut(s) 33, 569
MspR9I CCNGG 1 cut(s) 321
Mva1269I GAATGC 1 cut(s) 741
MvaI CCWGG 1 cut(s) 321
MvnI CGCG 1 cut(s) 11
MwoI GCNNNNNNNGC 3 cut(s) 33, 379, 711
NaeI GCCGGC 1 cut(s) 34
NcoI CCATGG 1 cut(s) 150
NdeII GATC 3 cut(s) 115, 729, 784
NgoMIV GCCGGC 1 cut(s) 32
NlaIII CATG 9 cut(s) 154, 235, 347, 466, 484, 506, 610, 670, 712
NlaIV GGNNCC 5 cut(s) 117, 207, 208, 573, 612
NmuCI GTSAC 2 cut(s) 439, 797
NspI RCATGY 2 cut(s) 347, 506
PciI ACATGT 1 cut(s) 502
PctI GAATGC 1 cut(s) 741
PdiI GCCGGC 1 cut(s) 34
PfeI GAWTC 3 cut(s) 419, 484, 598
PkrI GCNGC 6 cut(s) 15, 38, 41, 124, 381, 707
PpuMI RGGWCCY 1 cut(s) 206
PscI ACATGT 1 cut(s) 502
Psp5II RGGWCCY 1 cut(s) 206
Psp6I CCWGG 1 cut(s) 319
PspGI CCWGG 1 cut(s) 319
PspN4I GGNNCC 5 cut(s) 117, 207, 208, 573, 612
PspPI GGNCC 3 cut(s) 26, 206, 526
PspPPI RGGWCCY 1 cut(s) 206
PstI CTGCAG 1 cut(s) 381
PsuI RGATCY 1 cut(s) 115
PvuII CAGCTG 1 cut(s) 16
RsaI GTAC 2 cut(s) 79, 410
RsaNI GTAC 2 cut(s) 78, 409
SalI GTCGAC 1 cut(s) 46
SaqAI TTAA 3 cut(s) 108, 507, 774
SatI GCNGC 6 cut(s) 14, 37, 40, 123, 380, 706
Sau3AI GATC 3 cut(s) 115, 729, 784
Sau96I GGNCC 3 cut(s) 26, 206, 526
ScrFI CCNGG 1 cut(s) 321
SfaNI GCATC 3 cut(s) 382, 476, 748
SfcI CTRYAG 1 cut(s) 377
SinI GGWCC 2 cut(s) 206, 526
SmlI CTYRAG 3 cut(s) 242, 576, 691
SmoI CTYRAG 3 cut(s) 242, 576, 691
SpeI ACTAGT 1 cut(s) 649
Sse9I AATT 6 cut(s) 130, 275, 283, 388, 494, 779
SsiI CCGC 1 cut(s) 39
SspI AATATT 2 cut(s) 103, 511
SspMI CTAG 3 cut(s) 60, 650, 686
StyD4I CCNGG 1 cut(s) 319
StyI CCWWGG 2 cut(s) 150, 174
TaaI ACNGT 1 cut(s) 77
TaqI TCGA 3 cut(s) 47, 654, 732
TasI AATT 6 cut(s) 130, 275, 283, 388, 494, 779
TatI WGTACW 1 cut(s) 408
TauI GCSGC 1 cut(s) 42
TfiI GAWTC 3 cut(s) 419, 484, 598
Tru1I TTAA 3 cut(s) 108, 507, 774
Tru9I TTAA 3 cut(s) 108, 507, 774
TscAI CASTG 1 cut(s) 80
TseFI GTSAC 2 cut(s) 439, 797
TseI GCWGC 5 cut(s) 13, 36, 122, 379, 705
Tsp45I GTSAC 2 cut(s) 439, 797
TspDTI ATGAA 1 cut(s) 273
TspGWI ACGGA 2 cut(s) 305, 449
TspRI CASTG 1 cut(s) 80
VpaK11BI GGWCC 2 cut(s) 206, 526
XapI RAATTY 3 cut(s) 130, 388, 779
XceI RCATGY 2 cut(s) 347, 506
XmiI GTMKAC 1 cut(s) 47
XspI CTAG 3 cut(s) 60, 650, 686
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.