RLG00000020464

GPN-loop GTPase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0002226
Physical Location & Seq
Reverse (-)
65316658 .. 65335574
18917 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000020464

Sequence Viewer

Length: 687 bp
ATGAAATTAGGAATTGTCGGCAAAGAAGAAGCCCTAAAACCCCACGTTTCTAAAGCTCTTGGACCAGAAGAGCTCCGATTTCTGGATTCTGAAAGCCACAGAAGAACAAAACTCCTAGCAAGCAAAGAAAGCAAACCCAGATATCCGAAAATGGGTTATGCACAGCTAGTTATTGGCCTTGCAGGCAGTGGCAAGCTGCAGTATTGCAGATACAACGTACCATTTTTAGTTGTGGTTGCCAGAAGCCTTTTGATTGCTGAAACATTGAATTTACTCTTTTGCCAAATAGAATTTTTTTCTCATGTGCCTATGCTTCGGAACTTTGTGGACCATTTAAAGAGCAAGAGTTTCAACGTTTGTGCCGTATACTTGCTTGATTCACAGTTCATGACTGATGTGACCAAGTTTATCAGTGGGTGCATGGCATCTCTTTCTGCAATGGTTCAACTTGAATTACCGCATGTTAACATTCTCTCCAAAATGGACCTTGTGACGAACAAAAAGGATATTGAAGATTTCTTGAATCCAGAGCCCCAATTTTTGTTGTCAGAGTTGAACAAAAGCATGGCTCCTCAATTTGCAAAGCTTAACAAGTCTTTGATGGAACTGGTTGATGATTATAGCATGGTGAGTTTTGTGCCACTTGACTTGAGGAAGGAGAGCAGCATAAGATATGTATTGGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ATP_bind_1

Protein Analysis

229

Amino Acids

25.83

Weight (kDa)

8.79

Isoelectric Point (pI)

43.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ATP_bind_1 PF03029 95 - 227 1.8e-32 Conserved hypothetical ATP binding protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 366
AciI CCGC 1 cut(s) 458
AclI AACGTT 1 cut(s) 354
AcsI RAATTY 2 cut(s) 268, 290
AfaI GTAC 1 cut(s) 219
AfiI CCNNNNNNNGG 2 cut(s) 82, 152
AgsI TTSAA 7 cut(s) 268, 352, 446, 452, 512, 523, 556
AluBI AGCT 5 cut(s) 56, 73, 166, 196, 586
AluI AGCT 5 cut(s) 56, 73, 166, 196, 586
Alw21I GWGCWC 1 cut(s) 75
AoxI GGCC 1 cut(s) 175
ApeKI GCWGC 2 cut(s) 196, 663
ApoI RAATTY 2 cut(s) 268, 290
AspS9I GGNCC 3 cut(s) 62, 328, 484
AsuHPI GGTGA 1 cut(s) 640
AvaII GGWCC 3 cut(s) 62, 328, 484
BanII GRGCYC 2 cut(s) 75, 534
Bbv12I GWGCWC 1 cut(s) 75
BbvI GCAGC 2 cut(s) 183, 675
BccI CCATC 1 cut(s) 595
BceAI ACGGC 1 cut(s) 347
BfaI CTAG 2 cut(s) 116, 167
BfmI CTRYAG 1 cut(s) 197
BglI GCCNNNNNGGC 1 cut(s) 183
BisI GCNGC 2 cut(s) 197, 664
BlsI GCNGC 2 cut(s) 198, 665
Bme18I GGWCC 3 cut(s) 62, 328, 484
BmgT120I GGNCC 3 cut(s) 62, 328, 484
BmiI GGNNCC 1 cut(s) 570
BmsI GCATC 1 cut(s) 434
BpuEI CTTGAG 1 cut(s) 670
BsaXI ACNNNNNCTCC 2 cut(s) 458, 488
Bsc4I CCNNNNNNNGG 2 cut(s) 82, 152
Bse1I ACTGG 1 cut(s) 612
Bse3DI GCAATG 1 cut(s) 444
BseLI CCNNNNNNNGG 2 cut(s) 82, 152
BseMI GCAATG 1 cut(s) 444
BseNI ACTGG 1 cut(s) 612
BseRI GAGGAG 1 cut(s) 561
BseXI GCAGC 2 cut(s) 183, 675
BshFI GGCC 1 cut(s) 177
BsiHKAI GWGCWC 1 cut(s) 75
BslI CCNNNNNNNGG 2 cut(s) 82, 152
BsnI GGCC 1 cut(s) 177
Bsp1286I GDGCHC 2 cut(s) 75, 534
BspACI CCGC 1 cut(s) 458
BspANI GGCC 1 cut(s) 177
BspHI TCATGA 1 cut(s) 387
BspLI GGNNCC 1 cut(s) 570
BspMAI CTGCAG 1 cut(s) 201
BspQI GCTCTTC 1 cut(s) 63
BsrDI GCAATG 1 cut(s) 444
BsrI ACTGG 1 cut(s) 612
BssNAI GTATAC 1 cut(s) 367
Bst1107I GTATAC 1 cut(s) 367
Bst4CI ACNGT 1 cut(s) 384
Bst6I CTCTTC 1 cut(s) 63
BstC8I GCNNGC 3 cut(s) 121, 184, 194
BstMWI GCNNNNNNNGC 2 cut(s) 129, 183
BstNSI RCATGY 1 cut(s) 464
BstSFI CTRYAG 1 cut(s) 197
BstV1I GCAGC 2 cut(s) 183, 675
BstZ17I GTATAC 1 cut(s) 367
BsuRI GGCC 1 cut(s) 177
BtsI GCAGTG 1 cut(s) 193
BtsIMutI CAGTG 2 cut(s) 193, 418
Cac8I GCNNGC 3 cut(s) 121, 184, 194
CciI TCATGA 1 cut(s) 387
Cfr13I GGNCC 3 cut(s) 62, 328, 484
Csp6I GTAC 1 cut(s) 218
CspCI CAANNNNNGTGG 2 cut(s) 630, 665
CviAII CATG 6 cut(s) 302, 388, 421, 461, 565, 625
CviQI GTAC 1 cut(s) 218
DraI TTTAAA 1 cut(s) 336
Eam1104I CTCTTC 1 cut(s) 63
EarI CTCTTC 1 cut(s) 63
Ecl136II GAGCTC 1 cut(s) 73
Eco24I GRGCYC 2 cut(s) 75, 534
Eco32I GATATC 1 cut(s) 143
Eco47I GGWCC 3 cut(s) 62, 328, 484
Eco53kI GAGCTC 1 cut(s) 73
EcoICRI GAGCTC 1 cut(s) 73
EcoRV GATATC 1 cut(s) 143
EcoT38I GRGCYC 2 cut(s) 75, 534
FaeI CATG 6 cut(s) 305, 391, 424, 464, 568, 628
FatI CATG 6 cut(s) 301, 387, 420, 460, 564, 624
FblI GTMKAC 1 cut(s) 366
Fnu4HI GCNGC 2 cut(s) 197, 664
FriOI GRGCYC 2 cut(s) 75, 534
Fsp4HI GCNGC 2 cut(s) 197, 664
FspBI CTAG 2 cut(s) 116, 167
GluI GCNGC 2 cut(s) 197, 664
HaeIII GGCC 1 cut(s) 177
Hin1II CATG 6 cut(s) 305, 391, 424, 464, 568, 628
HincII GTYRAC 1 cut(s) 466
HindII GTYRAC 1 cut(s) 466
HindIII AAGCTT 1 cut(s) 584
HinfI GANTC 3 cut(s) 86, 377, 523
HpaI GTTAAC 1 cut(s) 466
HphI GGTGA 1 cut(s) 640
Hpy166II GTNNAC 3 cut(s) 328, 367, 466
Hpy188I TCNGA 5 cut(s) 77, 91, 147, 318, 550
Hpy188III TCNNGA 4 cut(s) 83, 388, 520, 527
Hpy8I GTNNAC 3 cut(s) 328, 367, 466
HpyAV CCTTC 1 cut(s) 649
HpyCH4III ACNGT 1 cut(s) 384
HpyCH4IV ACGT 3 cut(s) 45, 216, 354
HpyCH4V TGCA 7 cut(s) 161, 182, 199, 207, 420, 437, 581
HpyF10VI GCNNNNNNNGC 2 cut(s) 129, 183
HpySE526I ACGT 3 cut(s) 45, 216, 354
Hsp92II CATG 6 cut(s) 305, 391, 424, 464, 568, 628
KspAI GTTAAC 1 cut(s) 466
LguI GCTCTTC 1 cut(s) 63
LmnI GCTCC 2 cut(s) 78, 574
LpnPI CCDG 7 cut(s) 68, 78, 151, 168, 253, 540, 593
Lsp1109I GCAGC 2 cut(s) 183, 675
LweI GCATC 1 cut(s) 434
MaeI CTAG 2 cut(s) 116, 167
MaeII ACGT 3 cut(s) 45, 216, 354
MaeIII GTNAC 2 cut(s) 397, 490
MboII GAAGA 4 cut(s) 38, 80, 114, 524
MhlI GDGCHC 2 cut(s) 75, 534
MluCI AATT 7 cut(s) 5, 12, 268, 290, 452, 536, 575
MnlI CCTC 2 cut(s) 582, 645
MseI TTAA 4 cut(s) 335, 465, 588, 685
MwoI GCNNNNNNNGC 2 cut(s) 129, 183
NlaIII CATG 6 cut(s) 305, 391, 424, 464, 568, 628
NlaIV GGNNCC 1 cut(s) 570
NmuCI GTSAC 2 cut(s) 397, 490
NspI RCATGY 1 cut(s) 464
PagI TCATGA 1 cut(s) 387
PciSI GCTCTTC 1 cut(s) 63
PcsI WCGNNNNNNNCGW 1 cut(s) 360
PfeI GAWTC 3 cut(s) 86, 377, 523
PkrI GCNGC 2 cut(s) 198, 665
Psp124BI GAGCTC 1 cut(s) 75
Psp1406I AACGTT 1 cut(s) 354
PspN4I GGNNCC 1 cut(s) 570
PspPI GGNCC 3 cut(s) 62, 328, 484
PstI CTGCAG 1 cut(s) 201
RsaI GTAC 1 cut(s) 219
RsaNI GTAC 1 cut(s) 218
SacI GAGCTC 1 cut(s) 75
SapI GCTCTTC 1 cut(s) 63
SaqAI TTAA 4 cut(s) 335, 465, 588, 685
SatI GCNGC 2 cut(s) 197, 664
Sau96I GGNCC 3 cut(s) 62, 328, 484
SduI GDGCHC 2 cut(s) 75, 534
SetI ASST 9 cut(s) 48, 58, 75, 168, 198, 219, 357, 489, 588
SfaNI GCATC 1 cut(s) 434
SfcI CTRYAG 1 cut(s) 197
SinI GGWCC 3 cut(s) 62, 328, 484
SmlI CTYRAG 1 cut(s) 649
SmoI CTYRAG 1 cut(s) 649
Sse9I AATT 7 cut(s) 5, 12, 268, 290, 452, 536, 575
SsiI CCGC 1 cut(s) 458
SspMI CTAG 2 cut(s) 116, 167
SstI GAGCTC 1 cut(s) 75
TaaI ACNGT 1 cut(s) 384
TaiI ACGT 3 cut(s) 48, 219, 357
TasI AATT 7 cut(s) 5, 12, 268, 290, 452, 536, 575
TfiI GAWTC 3 cut(s) 86, 377, 523
Tru1I TTAA 4 cut(s) 335, 465, 588, 685
Tru9I TTAA 4 cut(s) 335, 465, 588, 685
TscAI CASTG 2 cut(s) 193, 418
TseFI GTSAC 2 cut(s) 397, 490
TseI GCWGC 2 cut(s) 196, 663
Tsp45I GTSAC 2 cut(s) 397, 490
TspDTI ATGAA 2 cut(s) 17, 376
TspRI CASTG 2 cut(s) 193, 418
VpaK11BI GGWCC 3 cut(s) 62, 328, 484
XapI RAATTY 2 cut(s) 268, 290
XceI RCATGY 1 cut(s) 464
XmiI GTMKAC 1 cut(s) 366
XspI CTAG 2 cut(s) 116, 167
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.