Rorug02G0418800

GPN-loop GTPase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0002226
Physical Location & Seq
Reverse (-)
53765783 .. 53766310
528 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0418800.1

Sequence Viewer

Length: 429 bp
ATGTTGTGTTGGCGAAAACCTTCTGTTGGCCAATTCAAATTAAATGTGGATGGATCCCGAACCAATAATGGTTTAATTGGTGCTGGAAGGGTCATAAGAGACAGCTCAGGAATTTGGCATCATGGCTTTATGCACAACATTGGTAGTGGTGAAGTTCTTCAAGCTGAAGCTTGGGGTTTGTTTACAGGTTTGCAGATTGCCATTGAGCTTAAGATCAAGCATATTACTGTGGAAACCGATTCTGCTACCCTTTTGGACCTAATGATGAGCACTGACATTGATCTCCATCCCTTAGCCATGAGAAGTATTGATCAGGAGTTAGGGGTCTGCAGATGTCCTTCCATTCCTCCTTTTGTCTATCAAGCTGTGATGGATGATACTGATGGCATTTCTAGGCCGAGAACCTTTATGTCTTATAAGATAGACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ATP_bind_1

Protein Analysis

142

Amino Acids

15.78

Weight (kDa)

5.91

Isoelectric Point (pI)

42.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_3 PF13456 15 - 91 2.3e-14 Reverse transcriptase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 417
AclWI GGATC 2 cut(s) 48, 61
AcoI YGGCCR 1 cut(s) 28
AcsI RAATTY 1 cut(s) 111
AcuI CTGAAG 1 cut(s) 186
AfiI CCNNNNNNNGG 1 cut(s) 26
AflII CTTAAG 1 cut(s) 209
AgsI TTSAA 2 cut(s) 37, 161
AluBI AGCT 5 cut(s) 105, 164, 170, 208, 365
AluI AGCT 5 cut(s) 105, 164, 170, 208, 365
Alw21I GWGCWC 1 cut(s) 272
Alw26I GTCTC 1 cut(s) 93
AlwI GGATC 2 cut(s) 48, 61
AoxI GGCC 2 cut(s) 28, 395
ApoI RAATTY 1 cut(s) 111
Asp700I GAANNNNTTC 2 cut(s) 19, 156
AspS9I GGNCC 1 cut(s) 256
AsuHPI GGTGA 1 cut(s) 161
AvaII GGWCC 1 cut(s) 256
BalI TGGCCA 1 cut(s) 30
BamHI GGATCC 1 cut(s) 53
Bbv12I GWGCWC 1 cut(s) 272
BccI CCATC 4 cut(s) 44, 294, 364, 377
BclI TGATCA 1 cut(s) 310
BcoDI GTCTC 1 cut(s) 93
BfaI CTAG 2 cut(s) 393, 427
BfmI CTRYAG 1 cut(s) 328
BfrI CTTAAG 1 cut(s) 209
Bme18I GGWCC 1 cut(s) 256
BmgT120I GGNCC 1 cut(s) 256
BmiI GGNNCC 1 cut(s) 55
BmsI GCATC 1 cut(s) 127
Bpu10I CCTNAGC 2 cut(s) 106, 292
BsaBI GATNNNNATC 1 cut(s) 285
Bsc4I CCNNNNNNNGG 1 cut(s) 26
Bse8I GATNNNNATC 1 cut(s) 285
BseGI GGATG 3 cut(s) 55, 286, 379
BseJI GATNNNNATC 1 cut(s) 285
BseLI CCNNNNNNNGG 1 cut(s) 26
BseMII CTCAG 1 cut(s) 120
BshFI GGCC 2 cut(s) 30, 397
BsiHKAI GWGCWC 1 cut(s) 272
BslI CCNNNNNNNGG 1 cut(s) 26
BsmAI GTCTC 1 cut(s) 93
BsnI GGCC 2 cut(s) 30, 397
Bsp1286I GDGCHC 1 cut(s) 272
Bsp143I GATC 4 cut(s) 53, 213, 280, 310
BspANI GGCC 2 cut(s) 30, 397
BspCNI CTCAG 1 cut(s) 119
BspLI GGNNCC 1 cut(s) 55
BspMAI CTGCAG 1 cut(s) 332
BspPI GGATC 2 cut(s) 48, 61
BspTI CTTAAG 1 cut(s) 209
BssMI GATC 4 cut(s) 53, 213, 280, 310
Bst4CI ACNGT 1 cut(s) 229
BstAFI CTTAAG 1 cut(s) 209
BstDEI CTNAG 2 cut(s) 106, 292
BstF5I GGATG 3 cut(s) 55, 286, 379
BstKTI GATC 4 cut(s) 56, 216, 283, 313
BstMAI GTCTC 1 cut(s) 93
BstMBI GATC 4 cut(s) 53, 213, 280, 310
BstSFI CTRYAG 1 cut(s) 328
BstX2I RGATCY 1 cut(s) 53
BstYI RGATCY 1 cut(s) 53
BsuRI GGCC 2 cut(s) 30, 397
BtsCI GGATG 3 cut(s) 55, 286, 379
BtsIMutI CAGTG 1 cut(s) 270
Cfr13I GGNCC 1 cut(s) 256
CviAII CATG 2 cut(s) 122, 298
CviJI RGCY 9 cut(s) 30, 105, 126, 164, 170, 208, 296, 365, 397
CviKI_1 RGCY 9 cut(s) 30, 105, 126, 164, 170, 208, 296, 365, 397
DdeI CTNAG 2 cut(s) 106, 292
DpnI GATC 4 cut(s) 55, 215, 282, 312
DpnII GATC 4 cut(s) 53, 213, 280, 310
EaeI YGGCCR 1 cut(s) 28
Eco47I GGWCC 1 cut(s) 256
Eco57I CTGAAG 1 cut(s) 186
FaeI CATG 2 cut(s) 125, 301
FaiI YATR 7 cut(s) 95, 123, 131, 222, 299, 410, 417
FatI CATG 2 cut(s) 121, 297
FbaI TGATCA 1 cut(s) 310
FokI GGATG 3 cut(s) 62, 273, 386
FspBI CTAG 2 cut(s) 393, 427
HaeIII GGCC 2 cut(s) 30, 397
Hin1II CATG 2 cut(s) 125, 301
HindIII AAGCTT 1 cut(s) 168
HinfI GANTC 1 cut(s) 239
HphI GGTGA 1 cut(s) 161
Hpy166II GTNNAC 1 cut(s) 183
Hpy188III TCNNGA 3 cut(s) 57, 108, 314
Hpy8I GTNNAC 1 cut(s) 183
HpyAV CCTTC 3 cut(s) 30, 81, 348
HpyCH4III ACNGT 1 cut(s) 229
HpyCH4V TGCA 3 cut(s) 133, 193, 330
HpyF3I CTNAG 2 cut(s) 106, 292
Hsp92II CATG 2 cut(s) 125, 301
Ksp22I TGATCA 1 cut(s) 310
Kzo9I GATC 4 cut(s) 53, 213, 280, 310
LpnPI CCDG 4 cut(s) 69, 93, 171, 299
LweI GCATC 1 cut(s) 127
MaeI CTAG 2 cut(s) 393, 427
MalI GATC 4 cut(s) 55, 215, 282, 312
MboI GATC 4 cut(s) 53, 213, 280, 310
MboII GAAGA 1 cut(s) 149
MflI RGATCY 1 cut(s) 53
MhlI GDGCHC 1 cut(s) 272
MlsI TGGCCA 1 cut(s) 30
MluCI AATT 4 cut(s) 32, 38, 75, 111
MluNI TGGCCA 1 cut(s) 30
MnlI CCTC 1 cut(s) 357
Mox20I TGGCCA 1 cut(s) 30
MroXI GAANNNNTTC 2 cut(s) 19, 156
MscI TGGCCA 1 cut(s) 30
MseI TTAA 3 cut(s) 41, 74, 210
Msp20I TGGCCA 1 cut(s) 30
MspCI CTTAAG 1 cut(s) 209
NdeII GATC 4 cut(s) 53, 213, 280, 310
NlaIII CATG 2 cut(s) 125, 301
NlaIV GGNNCC 1 cut(s) 55
NmeAIII GCCGAG 1 cut(s) 423
PdmI GAANNNNTTC 2 cut(s) 19, 156
PfeI GAWTC 1 cut(s) 239
PsiI TTATAA 1 cut(s) 417
PspN4I GGNNCC 1 cut(s) 55
PspPI GGNCC 1 cut(s) 256
PstI CTGCAG 1 cut(s) 332
PsuI RGATCY 1 cut(s) 53
SaqAI TTAA 3 cut(s) 41, 74, 210
Sau3AI GATC 4 cut(s) 53, 213, 280, 310
Sau96I GGNCC 1 cut(s) 256
SduI GDGCHC 1 cut(s) 272
SetI ASST 9 cut(s) 22, 107, 166, 172, 190, 210, 261, 367, 407
SfaNI GCATC 1 cut(s) 127
SfcI CTRYAG 1 cut(s) 328
SinI GGWCC 1 cut(s) 256
SmlI CTYRAG 1 cut(s) 209
SmoI CTYRAG 1 cut(s) 209
Sse9I AATT 4 cut(s) 32, 38, 75, 111
SspMI CTAG 2 cut(s) 393, 427
TaaI ACNGT 1 cut(s) 229
TasI AATT 4 cut(s) 32, 38, 75, 111
TfiI GAWTC 1 cut(s) 239
Tru1I TTAA 3 cut(s) 41, 74, 210
Tru9I TTAA 3 cut(s) 41, 74, 210
TscAI CASTG 1 cut(s) 277
TspRI CASTG 1 cut(s) 277
Vha464I CTTAAG 1 cut(s) 209
VpaK11BI GGWCC 1 cut(s) 256
XapI RAATTY 1 cut(s) 111
XmnI GAANNNNTTC 2 cut(s) 19, 156
XspI CTAG 2 cut(s) 393, 427
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.