FvH4_6g37261

GPN-loop GTPase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0002226
Physical Location & Seq
Reverse (-)
29362057 .. 29370456
8400 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g37261.t8

Sequence Viewer

Length: 813 bp
ATGGGTTATGCACAGCTAGTTATTGGCCCTGCTGGCAGTGGCAAGTCCACTTACTGCTCCAGTTTGCACCGACACTGTTTGGATAATGGGAGGACTCTACATATTGTTAATTTGGATCCTGCTGCGGAGCATTTTGACTACCCTGTGGCTTTGGATATCAGGGAGCTTATTAACTTGGATGATGTTATGGAGGAACTTGGACTAGGTCCCAATGGTGGACTTATGTACTGCATGGAACACCTTGAAGATAATCTGGATGATTGGCTTGCAGAGGGATTGGAGGATTACTTGGATGATGACTACTTAGTTTTCGACTGCCCAGGCCAGATAGAACTTTTTTCTCATGTGCCTATGCTTCGGAACTTTGTGGACCATTTGAAGAGCAAGAGTTTCAACGTCTGTGCCGTATACTTGCTTGATTCACAGTTCATGACTGATGTGACCAAGTTTATCAGTGGGTGCATGGCATCTCTTTCTGCTATGGTTCAACTTGAATTACCACATGTTAATATTCTCTCCAAAATGGATCTTGTGACGAACAAAAAGGATATTGAAGATTTCTTGAATCCGGAGCCCCGAGTTTTGTTGACAGAGTTGAACAAACGCATGGCTCCTCAGTTTGCAAAGCTTAACAAGGCCTTGATGGAACTGGTTGATGATTATAGCATGGTGAGTTTTGTGCCACTTGACTTGAGGAAGGAGAGCAGCATAAGATATGTTTTGGCTCAAATTGATACCTCGATTCAGTTTGGAGAAGATGCCGATGTGAAGGTTAAGGATTTTGATCCAGAAGCTCCAGAAGATGAAAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ATP_bind_1

Protein Analysis

271

Amino Acids

30.46

Weight (kDa)

4.4

Isoelectric Point (pI)

42.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ATP_bind_1 PF03029 7 - 249 2.8e-85 Conserved hypothetical ATP binding protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 408
AccIII TCCGGA 1 cut(s) 568
AciI CCGC 1 cut(s) 125
AclWI GGATC 4 cut(s) 110, 123, 534, 779
AfaI GTAC 1 cut(s) 227
AfiI CCNNNNNNNGG 1 cut(s) 215
AflIII ACRYGT 1 cut(s) 502
AgsI TTSAA 8 cut(s) 245, 379, 394, 488, 494, 554, 565, 598
AjnI CCWGG 1 cut(s) 319
AluBI AGCT 5 cut(s) 16, 166, 628, 794, 810
AluI AGCT 5 cut(s) 16, 166, 628, 794, 810
AlwI GGATC 4 cut(s) 110, 123, 534, 779
Ama87I CYCGRG 1 cut(s) 576
Aor13HI TCCGGA 1 cut(s) 568
AoxI GGCC 3 cut(s) 25, 322, 636
ApeKI GCWGC 2 cut(s) 122, 705
AspS9I GGNCC 3 cut(s) 26, 206, 370
AsuHPI GGTGA 1 cut(s) 682
AvaI CYCGRG 1 cut(s) 576
AvaII GGWCC 2 cut(s) 206, 370
BamHI GGATCC 1 cut(s) 115
BanII GRGCYC 1 cut(s) 576
BbvI GCAGC 2 cut(s) 109, 717
BccI CCATC 1 cut(s) 637
BceAI ACGGC 1 cut(s) 389
BciT130I CCWGG 1 cut(s) 321
BfaI CTAG 2 cut(s) 17, 203
BglI GCCNNNNNGGC 1 cut(s) 33
BisI GCNGC 2 cut(s) 123, 706
BlsI GCNGC 2 cut(s) 124, 707
Bme1390I CCNGG 1 cut(s) 321
Bme18I GGWCC 2 cut(s) 206, 370
BmeT110I CYCGRG 1 cut(s) 576
BmgT120I GGNCC 3 cut(s) 26, 206, 370
BmiI GGNNCC 4 cut(s) 117, 208, 573, 612
BmrFI CCNGG 1 cut(s) 321
BmsI GCATC 2 cut(s) 476, 748
BpmI CTGGAG 2 cut(s) 43, 780
BpuEI CTTGAG 1 cut(s) 712
BsaBI GATNNNNATC 1 cut(s) 783
BsaJI CCNNGG 1 cut(s) 319
BsaWI WCCGGW 1 cut(s) 568
Bsc4I CCNNNNNNNGG 1 cut(s) 215
Bse1I ACTGG 2 cut(s) 60, 654
Bse8I GATNNNNATC 1 cut(s) 783
BseAI TCCGGA 1 cut(s) 568
BseBI CCWGG 1 cut(s) 321
BseDI CCNNGG 1 cut(s) 319
BseGI GGATG 3 cut(s) 184, 262, 298
BseJI GATNNNNATC 1 cut(s) 783
BseLI CCNNNNNNNGG 1 cut(s) 215
BseMII CTCAG 1 cut(s) 629
BseNI ACTGG 2 cut(s) 60, 654
BseRI GAGGAG 1 cut(s) 603
BseXI GCAGC 2 cut(s) 109, 717
BshFI GGCC 3 cut(s) 27, 324, 638
BsiHKCI CYCGRG 1 cut(s) 576
BsiSI CCGG 1 cut(s) 569
BslFI GGGAC 1 cut(s) 192
BslI CCNNNNNNNGG 1 cut(s) 215
BsmFI GGGAC 1 cut(s) 192
BsnI GGCC 3 cut(s) 27, 324, 638
BsoBI CYCGRG 1 cut(s) 576
Bsp1286I GDGCHC 1 cut(s) 576
Bsp13I TCCGGA 1 cut(s) 568
Bsp143I GATC 3 cut(s) 115, 526, 784
BspACI CCGC 1 cut(s) 125
BspANI GGCC 3 cut(s) 27, 324, 638
BspCNI CTCAG 1 cut(s) 628
BspEI TCCGGA 1 cut(s) 568
BspHI TCATGA 1 cut(s) 429
BspLI GGNNCC 4 cut(s) 117, 208, 573, 612
BspPI GGATC 4 cut(s) 110, 123, 534, 779
BspQI GCTCTTC 1 cut(s) 374
BsrI ACTGG 2 cut(s) 60, 654
BssECI CCNNGG 1 cut(s) 319
BssMI GATC 3 cut(s) 115, 526, 784
BssNAI GTATAC 1 cut(s) 409
Bst1107I GTATAC 1 cut(s) 409
Bst2UI CCWGG 1 cut(s) 321
Bst4CI ACNGT 2 cut(s) 77, 426
Bst6I CTCTTC 1 cut(s) 374
BstC8I GCNNGC 2 cut(s) 34, 267
BstDEI CTNAG 2 cut(s) 304, 615
BstF5I GGATG 3 cut(s) 184, 262, 298
BstKTI GATC 3 cut(s) 118, 529, 787
BstMBI GATC 3 cut(s) 115, 526, 784
BstMWI GCNNNNNNNGC 1 cut(s) 33
BstNI CCWGG 1 cut(s) 321
BstNSI RCATGY 1 cut(s) 506
BstSCI CCNGG 1 cut(s) 319
BstV1I GCAGC 2 cut(s) 109, 717
BstX2I RGATCY 2 cut(s) 115, 526
BstYI RGATCY 2 cut(s) 115, 526
BstZ17I GTATAC 1 cut(s) 409
BsuRI GGCC 3 cut(s) 27, 324, 638
BtsCI GGATG 3 cut(s) 184, 262, 298
BtsI GCAGTG 1 cut(s) 43
BtsIMutI CAGTG 3 cut(s) 43, 73, 460
Cac8I GCNNGC 2 cut(s) 34, 267
CciI TCATGA 1 cut(s) 429
Cfr13I GGNCC 3 cut(s) 26, 206, 370
Csp6I GTAC 1 cut(s) 226
CspCI CAANNNNNGTGG 2 cut(s) 672, 707
CviAII CATG 7 cut(s) 232, 344, 430, 463, 503, 607, 667
CviQI GTAC 1 cut(s) 226
DdeI CTNAG 2 cut(s) 304, 615
DpnI GATC 3 cut(s) 117, 528, 786
DpnII GATC 3 cut(s) 115, 526, 784
Eam1104I CTCTTC 1 cut(s) 374
EarI CTCTTC 1 cut(s) 374
Eco147I AGGCCT 1 cut(s) 638
Eco24I GRGCYC 1 cut(s) 576
Eco32I GATATC 1 cut(s) 157
Eco47I GGWCC 2 cut(s) 206, 370
Eco88I CYCGRG 1 cut(s) 576
EcoO109I RGGNCCY 1 cut(s) 206
EcoRII CCWGG 1 cut(s) 319
EcoRV GATATC 1 cut(s) 157
EcoT38I GRGCYC 1 cut(s) 576
FaeI CATG 7 cut(s) 235, 347, 433, 466, 506, 610, 670
FaqI GGGAC 1 cut(s) 192
FatI CATG 7 cut(s) 231, 343, 429, 462, 502, 606, 666
FblI GTMKAC 1 cut(s) 408
Fnu4HI GCNGC 2 cut(s) 123, 706
FokI GGATG 3 cut(s) 191, 269, 305
FriOI GRGCYC 1 cut(s) 576
Fsp4HI GCNGC 2 cut(s) 123, 706
FspBI CTAG 2 cut(s) 17, 203
GluI GCNGC 2 cut(s) 123, 706
GsuI CTGGAG 2 cut(s) 43, 780
HaeIII GGCC 3 cut(s) 27, 324, 638
HapII CCGG 1 cut(s) 569
Hin1II CATG 7 cut(s) 235, 347, 433, 466, 506, 610, 670
HincII GTYRAC 1 cut(s) 588
HindII GTYRAC 1 cut(s) 588
HindIII AAGCTT 1 cut(s) 626
HinfI GANTC 4 cut(s) 94, 419, 565, 742
HpaII CCGG 1 cut(s) 569
HphI GGTGA 1 cut(s) 682
Hpy166II GTNNAC 5 cut(s) 48, 218, 370, 409, 588
Hpy188I TCNGA 1 cut(s) 360
Hpy188III TCNNGA 6 cut(s) 254, 430, 562, 569, 788, 797
Hpy8I GTNNAC 5 cut(s) 48, 218, 370, 409, 588
HpyAV CCTTC 2 cut(s) 691, 763
HpyCH4III ACNGT 2 cut(s) 77, 426
HpyCH4IV ACGT 1 cut(s) 396
HpyCH4V TGCA 6 cut(s) 11, 67, 231, 269, 462, 623
HpyF10VI GCNNNNNNNGC 1 cut(s) 33
HpyF3I CTNAG 2 cut(s) 304, 615
HpySE526I ACGT 1 cut(s) 396
Hsp92II CATG 7 cut(s) 235, 347, 433, 466, 506, 610, 670
Kpn2I TCCGGA 1 cut(s) 568
Kzo9I GATC 3 cut(s) 115, 526, 784
LguI GCTCTTC 1 cut(s) 374
LmnI GCTCC 6 cut(s) 62, 127, 163, 571, 616, 799
Lsp1109I GCAGC 2 cut(s) 109, 717
LweI GCATC 2 cut(s) 476, 748
MaeI CTAG 2 cut(s) 17, 203
MaeII ACGT 1 cut(s) 396
MaeIII GTNAC 2 cut(s) 439, 532
MalI GATC 3 cut(s) 117, 528, 786
MboI GATC 3 cut(s) 115, 526, 784
MboII GAAGA 5 cut(s) 257, 391, 566, 767, 812
MflI RGATCY 2 cut(s) 115, 526
MhlI GDGCHC 1 cut(s) 576
MluCI AATT 3 cut(s) 109, 494, 729
MlyI GAGTC 1 cut(s) 88
MnlI CCTC 7 cut(s) 84, 184, 265, 274, 624, 687, 748
MroI TCCGGA 1 cut(s) 568
MseI TTAA 5 cut(s) 108, 171, 507, 630, 774
MspI CCGG 1 cut(s) 569
MspR9I CCNGG 1 cut(s) 321
MvaI CCWGG 1 cut(s) 321
MwoI GCNNNNNNNGC 1 cut(s) 33
NdeII GATC 3 cut(s) 115, 526, 784
NlaIII CATG 7 cut(s) 235, 347, 433, 466, 506, 610, 670
NlaIV GGNNCC 4 cut(s) 117, 208, 573, 612
NmuCI GTSAC 2 cut(s) 439, 532
NspI RCATGY 1 cut(s) 506
PagI TCATGA 1 cut(s) 429
PceI AGGCCT 1 cut(s) 638
PciI ACATGT 1 cut(s) 502
PciSI GCTCTTC 1 cut(s) 374
PcsI WCGNNNNNNNCGW 1 cut(s) 402
PfeI GAWTC 3 cut(s) 419, 565, 742
PflFI GACNNNGTC 1 cut(s) 204
PkrI GCNGC 2 cut(s) 124, 707
PleI GAGTC 1 cut(s) 88
PpsI GAGTC 1 cut(s) 88
PpuMI RGGWCCY 1 cut(s) 206
PscI ACATGT 1 cut(s) 502
Psp5II RGGWCCY 1 cut(s) 206
Psp6I CCWGG 1 cut(s) 319
PspGI CCWGG 1 cut(s) 319
PspN4I GGNNCC 4 cut(s) 117, 208, 573, 612
PspPI GGNCC 3 cut(s) 26, 206, 370
PspPPI RGGWCCY 1 cut(s) 206
PsuI RGATCY 2 cut(s) 115, 526
PsyI GACNNNGTC 1 cut(s) 204
RsaI GTAC 1 cut(s) 227
RsaNI GTAC 1 cut(s) 226
SapI GCTCTTC 1 cut(s) 374
SaqAI TTAA 5 cut(s) 108, 171, 507, 630, 774
SatI GCNGC 2 cut(s) 123, 706
Sau3AI GATC 3 cut(s) 115, 526, 784
Sau96I GGNCC 3 cut(s) 26, 206, 370
SchI GAGTC 1 cut(s) 88
ScrFI CCNGG 1 cut(s) 321
SduI GDGCHC 1 cut(s) 576
SfaNI GCATC 2 cut(s) 476, 748
SinI GGWCC 2 cut(s) 206, 370
SmlI CTYRAG 1 cut(s) 691
SmoI CTYRAG 1 cut(s) 691
Sse9I AATT 3 cut(s) 109, 494, 729
SseBI AGGCCT 1 cut(s) 638
SsiI CCGC 1 cut(s) 125
SspI AATATT 1 cut(s) 511
SspMI CTAG 2 cut(s) 17, 203
StuI AGGCCT 1 cut(s) 638
StyD4I CCNGG 1 cut(s) 319
TaaI ACNGT 2 cut(s) 77, 426
TaiI ACGT 1 cut(s) 399
TaqI TCGA 2 cut(s) 312, 740
TasI AATT 3 cut(s) 109, 494, 729
TatI WGTACW 1 cut(s) 225
TfiI GAWTC 3 cut(s) 419, 565, 742
Tru1I TTAA 5 cut(s) 108, 171, 507, 630, 774
Tru9I TTAA 5 cut(s) 108, 171, 507, 630, 774
TscAI CASTG 3 cut(s) 43, 80, 460
TseFI GTSAC 2 cut(s) 439, 532
TseI GCWGC 2 cut(s) 122, 705
Tsp45I GTSAC 2 cut(s) 439, 532
TspDTI ATGAA 1 cut(s) 418
TspRI CASTG 3 cut(s) 43, 80, 460
Tth111I GACNNNGTC 1 cut(s) 204
VpaK11BI GGWCC 2 cut(s) 206, 370
XceI RCATGY 1 cut(s) 506
XmiI GTMKAC 1 cut(s) 408
XspI CTAG 2 cut(s) 17, 203
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.