Rorug02G0418700

GPN-loop GTPase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0002226
Physical Location & Seq
Forward (+)
53746357 .. 53747167
811 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0418700.1

Sequence Viewer

Length: 591 bp
ATGCAGGAGAGTGGGAAGGACAATGCAAGTGGGAAGAGGAAGACCATAGTATTGGGACCATGGGGAGGCAATGGCGGAAACGTTTGGGATGATGGGCTCTATCATGGGGTGAGAGAAATCACTCTCGTCTATGGCCATTGCATCGACTCCATTGTTGTGGTGTATGATAAGAACGGTAAGCCGGTCACATCAGAAAAGCATGGTGGTCTTGGAGGCAATCGAACAGCTGAGATAAAGTTGCAGTACCCCGATGAGTTCCTAGTGAGTGTGAGCGGACACTATTGCCCGGTGGTGTATGGTGGCAGCCCGGTGATCCGGTCACTCAAATTTGAGAGTAATAGAAGAACGTTTGGACCATTTGGAGTTGAAGAAGGCACACCCTTTACTTTCACTGTGGATGGAGGCAAAATTGTTGGGTTAAAGGGAAGAGGTGGTTGGTACCTAGATGCAGTTGGGTTTCACGTATACCATGCTCCCAAAGGAAAGCTCTTTCGGAGAGTTCAGAAAAGTTTCCGAAGGCTTGCCAGCACGGTTTCAAAACCCCTGGCCACCAAAGAAACTGAAAAGACTACTGCTGAGAGTCGAAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ATP_bind_1

Protein Analysis

196

Amino Acids

21.42

Weight (kDa)

9.56

Isoelectric Point (pI)

37.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Jacalin PF01419 18 - 154 7.4e-44 Jacalin-like lectin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 438
AccB1I GGYRCC 1 cut(s) 438
AccBSI CCGCTC 1 cut(s) 273
AccI GTMKAC 1 cut(s) 465
AciI CCGC 2 cut(s) 75, 273
AclI AACGTT 2 cut(s) 81, 347
AclWI GGATC 1 cut(s) 307
AcoI YGGCCR 2 cut(s) 133, 546
AcsI RAATTY 1 cut(s) 326
AfaI GTAC 2 cut(s) 245, 440
AfiI CCNNNNNNNGG 1 cut(s) 65
AgsI TTSAA 2 cut(s) 368, 537
AjnI CCWGG 1 cut(s) 543
AjuI GAANNNNNNNTTGG 2 cut(s) 418, 450
AluBI AGCT 2 cut(s) 227, 487
AluI AGCT 2 cut(s) 227, 487
AlwI GGATC 1 cut(s) 307
AoxI GGCC 2 cut(s) 133, 546
ApeKI GCWGC 1 cut(s) 303
ApoI RAATTY 1 cut(s) 326
Asp700I GAANNNNTTC 1 cut(s) 509
Asp718I GGTACC 1 cut(s) 438
AspS9I GGNCC 2 cut(s) 56, 353
AsuC2I CCSGG 2 cut(s) 287, 308
AsuHPI GGTGA 2 cut(s) 121, 322
AvaII GGWCC 2 cut(s) 56, 353
BalI TGGCCA 2 cut(s) 135, 548
BanI GGYRCC 1 cut(s) 438
BanII GRGCYC 1 cut(s) 99
BbsI GAAGAC 1 cut(s) 47
BbvI GCAGC 1 cut(s) 315
BccI CCATC 2 cut(s) 86, 392
BciT130I CCWGG 1 cut(s) 545
BcnI CCSGG 2 cut(s) 287, 308
BfaI CTAG 2 cut(s) 260, 443
BisI GCNGC 1 cut(s) 304
BlsI GCNGC 1 cut(s) 305
Bme1390I CCNGG 3 cut(s) 287, 308, 545
Bme18I GGWCC 2 cut(s) 56, 353
BmgT120I GGNCC 2 cut(s) 56, 353
BmiI GGNNCC 2 cut(s) 57, 440
BmrFI CCNGG 3 cut(s) 287, 308, 545
BmsI GCATC 2 cut(s) 150, 436
BpiI GAAGAC 1 cut(s) 47
BpuMI CCSGG 2 cut(s) 287, 308
BsaAI YACGTR 1 cut(s) 463
BsaJI CCNNGG 2 cut(s) 59, 543
BsaWI WCCGGW 1 cut(s) 315
Bsc4I CCNNNNNNNGG 1 cut(s) 65
Bse118I RCCGGY 1 cut(s) 181
Bse3DI GCAATG 2 cut(s) 76, 136
BseBI CCWGG 1 cut(s) 545
BseDI CCNNGG 2 cut(s) 59, 543
BseGI GGATG 2 cut(s) 94, 403
BseLI CCNNNNNNNGG 1 cut(s) 65
BseMI GCAATG 2 cut(s) 76, 136
BseMII CTCAG 2 cut(s) 219, 567
BseXI GCAGC 1 cut(s) 315
BshFI GGCC 2 cut(s) 135, 548
BshNI GGYRCC 1 cut(s) 438
BsiSI CCGG 4 cut(s) 182, 287, 308, 316
BslFI GGGAC 1 cut(s) 69
BslI CCNNNNNNNGG 1 cut(s) 65
BsmFI GGGAC 1 cut(s) 69
BsnI GGCC 2 cut(s) 135, 548
Bsp1286I GDGCHC 1 cut(s) 99
Bsp143I GATC 1 cut(s) 312
Bsp19I CCATGG 1 cut(s) 59
BspACI CCGC 2 cut(s) 75, 273
BspANI GGCC 2 cut(s) 135, 548
BspCNI CTCAG 2 cut(s) 220, 568
BspLI GGNNCC 2 cut(s) 57, 440
BspPI GGATC 1 cut(s) 307
BspT107I GGYRCC 1 cut(s) 438
BsrBI CCGCTC 1 cut(s) 273
BsrDI GCAATG 2 cut(s) 76, 136
BsrFI RCCGGY 1 cut(s) 181
BssAI RCCGGY 1 cut(s) 181
BssECI CCNNGG 2 cut(s) 59, 543
BssMI GATC 1 cut(s) 312
BssNAI GTATAC 1 cut(s) 466
BssT1I CCWWGG 1 cut(s) 59
Bst1107I GTATAC 1 cut(s) 466
Bst2UI CCWGG 1 cut(s) 545
Bst4CI ACNGT 3 cut(s) 176, 394, 532
Bst6I CTCTTC 2 cut(s) 29, 421
BstBAI YACGTR 1 cut(s) 463
BstC8I GCNNGC 2 cut(s) 522, 526
BstDEI CTNAG 2 cut(s) 228, 576
BstDSI CCRYGG 1 cut(s) 59
BstF5I GGATG 2 cut(s) 94, 403
BstKTI GATC 1 cut(s) 315
BstMBI GATC 1 cut(s) 312
BstNI CCWGG 1 cut(s) 545
BstSCI CCNGG 3 cut(s) 285, 306, 543
BstV1I GCAGC 1 cut(s) 315
BstV2I GAAGAC 1 cut(s) 47
BstXI CCANNNNNNTGG 2 cut(s) 52, 157
BstZ17I GTATAC 1 cut(s) 466
BsuRI GGCC 2 cut(s) 135, 548
BtgI CCRYGG 1 cut(s) 59
BtsCI GGATG 2 cut(s) 94, 403
BtsIMutI CAGTG 1 cut(s) 390
Cac8I GCNNGC 2 cut(s) 522, 526
Cfr10I RCCGGY 1 cut(s) 181
Cfr13I GGNCC 2 cut(s) 56, 353
Csp6I GTAC 2 cut(s) 244, 439
CspCI CAANNNNNGTGG 2 cut(s) 10, 45
CviAII CATG 4 cut(s) 60, 104, 200, 470
CviJI RGCY 8 cut(s) 97, 135, 181, 227, 306, 487, 520, 548
CviKI_1 RGCY 8 cut(s) 97, 135, 181, 227, 306, 487, 520, 548
CviQI GTAC 2 cut(s) 244, 439
DdeI CTNAG 2 cut(s) 228, 576
DpnI GATC 1 cut(s) 314
DpnII GATC 1 cut(s) 312
EaeI YGGCCR 2 cut(s) 133, 546
Eam1104I CTCTTC 2 cut(s) 29, 421
EarI CTCTTC 2 cut(s) 29, 421
EciI GGCGGA 1 cut(s) 90
Eco130I CCWWGG 1 cut(s) 59
Eco24I GRGCYC 1 cut(s) 99
Eco47I GGWCC 2 cut(s) 56, 353
EcoRII CCWGG 1 cut(s) 543
EcoT14I CCWWGG 1 cut(s) 59
EcoT38I GRGCYC 1 cut(s) 99
ErhI CCWWGG 1 cut(s) 59
FaeI CATG 4 cut(s) 63, 107, 203, 473
FaiI YATR 9 cut(s) 47, 61, 105, 132, 165, 201, 297, 466, 471
FaqI GGGAC 1 cut(s) 69
FatI CATG 4 cut(s) 59, 103, 199, 469
FblI GTMKAC 1 cut(s) 465
Fnu4HI GCNGC 1 cut(s) 304
FokI GGATG 2 cut(s) 101, 410
FriOI GRGCYC 1 cut(s) 99
Fsp4HI GCNGC 1 cut(s) 304
FspBI CTAG 2 cut(s) 260, 443
GluI GCNGC 1 cut(s) 304
HaeIII GGCC 2 cut(s) 135, 548
HapII CCGG 4 cut(s) 182, 287, 308, 316
Hin1II CATG 4 cut(s) 63, 107, 203, 473
HinfI GANTC 2 cut(s) 146, 580
HpaII CCGG 4 cut(s) 182, 287, 308, 316
HphI GGTGA 2 cut(s) 121, 322
Hpy166II GTNNAC 1 cut(s) 466
Hpy188I TCNGA 4 cut(s) 193, 495, 504, 515
Hpy8I GTNNAC 1 cut(s) 466
HpyAV CCTTC 3 cut(s) 10, 365, 510
HpyCH4III ACNGT 3 cut(s) 176, 394, 532
HpyCH4IV ACGT 3 cut(s) 81, 347, 462
HpyCH4V TGCA 5 cut(s) 4, 26, 141, 241, 449
HpyF3I CTNAG 2 cut(s) 228, 576
HpySE526I ACGT 3 cut(s) 81, 347, 462
Hsp92II CATG 4 cut(s) 63, 107, 203, 473
KpnI GGTACC 1 cut(s) 442
Kzo9I GATC 1 cut(s) 312
LmnI GCTCC 1 cut(s) 478
LpnPI CCDG 7 cut(s) 195, 300, 321, 329, 530, 538, 557
Lsp1109I GCAGC 1 cut(s) 315
LweI GCATC 2 cut(s) 150, 436
MaeI CTAG 2 cut(s) 260, 443
MaeII ACGT 3 cut(s) 81, 347, 462
MaeIII GTNAC 2 cut(s) 184, 318
MalI GATC 1 cut(s) 314
MbiI CCGCTC 1 cut(s) 273
MboI GATC 1 cut(s) 312
MboII GAAGA 5 cut(s) 46, 52, 354, 380, 438
MhlI GDGCHC 1 cut(s) 99
MlsI TGGCCA 2 cut(s) 135, 548
MluCI AATT 3 cut(s) 326, 408, 586
MluNI TGGCCA 2 cut(s) 135, 548
MlyI GAGTC 2 cut(s) 140, 589
MnlI CCTC 5 cut(s) 30, 59, 206, 395, 422
Mox20I TGGCCA 2 cut(s) 135, 548
MroXI GAANNNNTTC 1 cut(s) 509
MscI TGGCCA 2 cut(s) 135, 548
MseI TTAA 2 cut(s) 419, 589
MslI CAYNNNNRTG 1 cut(s) 155
Msp20I TGGCCA 2 cut(s) 135, 548
MspA1I CMGCKG 1 cut(s) 227
MspI CCGG 4 cut(s) 182, 287, 308, 316
MspR9I CCNGG 3 cut(s) 287, 308, 545
MvaI CCWGG 1 cut(s) 545
NciI CCSGG 2 cut(s) 287, 308
NcoI CCATGG 1 cut(s) 59
NdeII GATC 1 cut(s) 312
NlaIII CATG 4 cut(s) 63, 107, 203, 473
NlaIV GGNNCC 2 cut(s) 57, 440
NmuCI GTSAC 2 cut(s) 184, 318
PdmI GAANNNNTTC 1 cut(s) 509
PkrI GCNGC 1 cut(s) 305
PleI GAGTC 2 cut(s) 140, 588
PpsI GAGTC 2 cut(s) 140, 588
Ppu21I YACGTR 1 cut(s) 463
Psp1406I AACGTT 2 cut(s) 81, 347
Psp6I CCWGG 1 cut(s) 543
PspGI CCWGG 1 cut(s) 543
PspN4I GGNNCC 2 cut(s) 57, 440
PspPI GGNCC 2 cut(s) 56, 353
PvuII CAGCTG 1 cut(s) 227
RsaI GTAC 2 cut(s) 245, 440
RsaNI GTAC 2 cut(s) 244, 439
RseI CAYNNNNRTG 1 cut(s) 155
SaqAI TTAA 2 cut(s) 419, 589
SatI GCNGC 1 cut(s) 304
Sau3AI GATC 1 cut(s) 312
Sau96I GGNCC 2 cut(s) 56, 353
SchI GAGTC 2 cut(s) 140, 589
ScrFI CCNGG 3 cut(s) 287, 308, 545
SduI GDGCHC 1 cut(s) 99
SetI ASST 7 cut(s) 84, 229, 350, 433, 444, 465, 489
SfaNI GCATC 2 cut(s) 150, 436
SinI GGWCC 2 cut(s) 56, 353
SmiMI CAYNNNNRTG 1 cut(s) 155
Sse9I AATT 3 cut(s) 326, 408, 586
SsiI CCGC 2 cut(s) 75, 273
SspMI CTAG 2 cut(s) 260, 443
StyD4I CCNGG 3 cut(s) 285, 306, 543
StyI CCWWGG 1 cut(s) 59
TaaI ACNGT 3 cut(s) 176, 394, 532
TaiI ACGT 3 cut(s) 84, 350, 465
TaqI TCGA 3 cut(s) 144, 220, 583
TasI AATT 3 cut(s) 326, 408, 586
Tru1I TTAA 2 cut(s) 419, 589
Tru9I TTAA 2 cut(s) 419, 589
TscAI CASTG 1 cut(s) 397
TseFI GTSAC 2 cut(s) 184, 318
TseI GCWGC 1 cut(s) 303
Tsp45I GTSAC 2 cut(s) 184, 318
TspRI CASTG 1 cut(s) 397
VpaK11BI GGWCC 2 cut(s) 56, 353
XapI RAATTY 1 cut(s) 326
XmiI GTMKAC 1 cut(s) 465
XmnI GAANNNNTTC 1 cut(s) 509
XspI CTAG 2 cut(s) 260, 443
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.