pycom17g13810

GPN-loop GTPase 3

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0002226
Physical Location & Seq
Forward (+)
11377333 .. 11379062
1730 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom17g13810.1

Sequence Viewer

Length: 339 bp
ATGGCATCTCTTTCTGCCATGATTCAACTTGAATTACCACATGTTAATATTCTCTCCAAAATGGACCTTGCACCGAGAAAAAAGGATATTGAAGACTTCTTGTATCCGGAGCCTCAAGTTTTATTGTCAGAGTTGAATCAACGCATGGCTCCTCAGTTTGCAAAGCTAAATAAATCTTTGATTGAACTAGTCGATGATTATAGCATGGTGAGTTTTGTGCCACTAGACTTGAGGAAGTCAAGCAGCATGAGCTATGTCTTGGCTCAGATTGATACCTGCATTCAGTTCGGAGAAGATGCCGATGTGAAGGTTAAGGAATTTGATCAGGAAGATGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ATP_bind_1

Protein Analysis

113

Amino Acids

12.78

Weight (kDa)

4.44

Isoelectric Point (pI)

53.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ATP_bind_1 PF03029 1 - 95 1.7e-15 Conserved hypothetical ATP binding protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 284
AccIII TCCGGA 1 cut(s) 106
AcsI RAATTY 1 cut(s) 317
AflIII ACRYGT 1 cut(s) 40
AgsI TTSAA 5 cut(s) 26, 32, 92, 136, 185
AhlI ACTAGT 1 cut(s) 187
AluBI AGCT 2 cut(s) 166, 252
AluI AGCT 2 cut(s) 166, 252
Aor13HI TCCGGA 1 cut(s) 106
ApeKI GCWGC 1 cut(s) 243
ApoI RAATTY 1 cut(s) 317
AspS9I GGNCC 1 cut(s) 64
AsuHPI GGTGA 1 cut(s) 220
AvaII GGWCC 1 cut(s) 64
BbsI GAAGAC 1 cut(s) 99
BbvI GCAGC 1 cut(s) 255
BcgI CGANNNNNNTGC 2 cut(s) 268, 302
BciVI GTATCC 1 cut(s) 114
BclI TGATCA 1 cut(s) 322
BcuI ACTAGT 1 cut(s) 187
BfaI CTAG 2 cut(s) 188, 224
BfuAI ACCTGC 1 cut(s) 284
BfuI GTATCC 1 cut(s) 114
BisI GCNGC 1 cut(s) 244
BlsI GCNGC 1 cut(s) 245
Bme18I GGWCC 1 cut(s) 64
BmgT120I GGNCC 1 cut(s) 64
BmiI GGNNCC 2 cut(s) 111, 150
BmsI GCATC 2 cut(s) 14, 286
BpiI GAAGAC 1 cut(s) 99
BpuEI CTTGAG 2 cut(s) 99, 250
BsaWI WCCGGW 1 cut(s) 106
BseAI TCCGGA 1 cut(s) 106
BseMII CTCAG 2 cut(s) 167, 278
BseRI GAGGAG 1 cut(s) 141
BseXI GCAGC 1 cut(s) 255
BsiSI CCGG 1 cut(s) 107
BsmI GAATGC 1 cut(s) 279
Bsp13I TCCGGA 1 cut(s) 106
Bsp143I GATC 1 cut(s) 322
BspCNI CTCAG 2 cut(s) 166, 277
BspEI TCCGGA 1 cut(s) 106
BspLI GGNNCC 2 cut(s) 111, 150
BspMI ACCTGC 1 cut(s) 284
BssMI GATC 1 cut(s) 322
BstDEI CTNAG 2 cut(s) 153, 264
BstKTI GATC 1 cut(s) 325
BstMBI GATC 1 cut(s) 322
BstMWI GCNNNNNNNGC 1 cut(s) 249
BstNSI RCATGY 1 cut(s) 44
BstV1I GCAGC 1 cut(s) 255
BstV2I GAAGAC 1 cut(s) 99
BsuI GTATCC 1 cut(s) 114
BveI ACCTGC 1 cut(s) 284
Cfr13I GGNCC 1 cut(s) 64
CspCI CAANNNNNGTGG 2 cut(s) 210, 245
CviAII CATG 5 cut(s) 19, 41, 145, 205, 247
CviJI RGCY 5 cut(s) 112, 149, 166, 252, 263
CviKI_1 RGCY 5 cut(s) 112, 149, 166, 252, 263
DdeI CTNAG 2 cut(s) 153, 264
DpnI GATC 1 cut(s) 324
DpnII GATC 1 cut(s) 322
Eco47I GGWCC 1 cut(s) 64
FaeI CATG 5 cut(s) 22, 44, 148, 208, 250
FaiI YATR 7 cut(s) 20, 42, 146, 201, 206, 248, 255
FatI CATG 5 cut(s) 18, 40, 144, 204, 246
FbaI TGATCA 1 cut(s) 322
Fnu4HI GCNGC 1 cut(s) 244
Fsp4HI GCNGC 1 cut(s) 244
FspBI CTAG 2 cut(s) 188, 224
GluI GCNGC 1 cut(s) 244
HapII CCGG 1 cut(s) 107
Hin1II CATG 5 cut(s) 22, 44, 148, 208, 250
HinfI GANTC 2 cut(s) 22, 136
HpaII CCGG 1 cut(s) 107
HphI GGTGA 1 cut(s) 220
Hpy188I TCNGA 3 cut(s) 130, 267, 290
Hpy188III TCNNGA 2 cut(s) 107, 326
HpyAV CCTTC 1 cut(s) 301
HpyCH4V TGCA 3 cut(s) 71, 161, 279
HpyF10VI GCNNNNNNNGC 1 cut(s) 249
HpyF3I CTNAG 2 cut(s) 153, 264
Hsp92II CATG 5 cut(s) 22, 44, 148, 208, 250
Kpn2I TCCGGA 1 cut(s) 106
Ksp22I TGATCA 1 cut(s) 322
Kzo9I GATC 1 cut(s) 322
LmnI GCTCC 2 cut(s) 109, 154
LpnPI CCDG 3 cut(s) 120, 289, 311
Lsp1109I GCAGC 1 cut(s) 255
LweI GCATC 2 cut(s) 14, 286
MaeI CTAG 2 cut(s) 188, 224
MalI GATC 1 cut(s) 324
MboI GATC 1 cut(s) 322
MboII GAAGA 2 cut(s) 104, 305
MluCI AATT 2 cut(s) 32, 317
MnlI CCTC 3 cut(s) 123, 162, 225
MroI TCCGGA 1 cut(s) 106
MseI TTAA 2 cut(s) 45, 312
MspI CCGG 1 cut(s) 107
Mva1269I GAATGC 1 cut(s) 279
MwoI GCNNNNNNNGC 1 cut(s) 249
NdeII GATC 1 cut(s) 322
NlaIII CATG 5 cut(s) 22, 44, 148, 208, 250
NlaIV GGNNCC 2 cut(s) 111, 150
NspI RCATGY 1 cut(s) 44
PciI ACATGT 1 cut(s) 40
PctI GAATGC 1 cut(s) 279
PfeI GAWTC 2 cut(s) 22, 136
PkrI GCNGC 1 cut(s) 245
PscI ACATGT 1 cut(s) 40
PspN4I GGNNCC 2 cut(s) 111, 150
PspPI GGNCC 1 cut(s) 64
SaqAI TTAA 2 cut(s) 45, 312
SatI GCNGC 1 cut(s) 244
Sau3AI GATC 1 cut(s) 322
Sau96I GGNCC 1 cut(s) 64
SetI ASST 5 cut(s) 69, 168, 254, 278, 312
SfaNI GCATC 2 cut(s) 14, 286
SinI GGWCC 1 cut(s) 64
SmlI CTYRAG 2 cut(s) 114, 229
SmoI CTYRAG 2 cut(s) 114, 229
SpeI ACTAGT 1 cut(s) 187
Sse9I AATT 2 cut(s) 32, 317
SspI AATATT 1 cut(s) 49
SspMI CTAG 2 cut(s) 188, 224
TaqI TCGA 1 cut(s) 192
TasI AATT 2 cut(s) 32, 317
TfiI GAWTC 2 cut(s) 22, 136
Tru1I TTAA 2 cut(s) 45, 312
Tru9I TTAA 2 cut(s) 45, 312
TseI GCWGC 1 cut(s) 243
VpaK11BI GGWCC 1 cut(s) 64
XapI RAATTY 1 cut(s) 317
XceI RCATGY 1 cut(s) 44
XspI CTAG 2 cut(s) 188, 224
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.