Rorug02G0419000

GPN-loop GTPase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0002226
Physical Location & Seq
Forward (+)
53776053 .. 53785027
8975 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0419000.1

Sequence Viewer

Length: 2976 bp
ATGGCTGAAACACTCGTTAATCTACTCCTGGATCGTTTGGCTTCGGTCACCACTGACAAGATCGGAAGAGAGCTGAAGCTGGTGATGGGTGTTGATGATGAAGTCGCAAATCTGTCCCGCAATTTGAAACTTATCAGAGCTGTGCTTCAGGATGCAGAGGAGAAACATGTCATCGAGGCCAGCATGGGAGAAGTGACAGGGGCCAGCGTGGGACACTGGCTGAATGAACTGAACCAAGTTTCTCATGAGATGGACGACGTGTTGGATGAGTGGAGCACAGAGATTCTTAAGCAGCAATTACTGGCGGATGAACAAGAAGGTTTGAACTCAACGGAGGCCTTGGAGATTCCGGAGGGTTCAAACAAAGGGGAAATTATTGAAACTGCTCCTCGTACTAAGGAGAAGGTATGCCTCCCCCTTCCTTCTTGCTTTTCTTGCTGCCGAGTCAAACAAGTAGTTCAGCGTCCAGTCAATATACTCCATCAAGTAGTTCATCGTCGCGACATTGCGGTCAAAATACAACGTCTAGACGAAAGGTTAACTCAGATTGCTAATGAAAGACAGCGTTATAACTTTCAAGATACAAAAGAAGCACCTAAACCACCTCCACGACAACAAACTTCTTCTCTTGTCATTGACAAATCCTTTGGTCGAGAAACCGAAAAGGACCTTTTAGTACTCAAGTTGCTAGATGAGAGTAGTAGTCATAAGAGGAGGGGTCCTCTTATCATCCCTATAATAGGGTTGGGAGGGATAGGGAAAACAACTCTCTGTCAACTAGTCTATAATGATGACAAGGTCAAGGCCCATTTTGAGAAGAGGATATGGGTCTGTGTCTCAGACCCTTTTGAAGTGATTACGATTGCTAAAGCCATCATAGAAAGTCTTGGTAATGTGAGCAATACTCCAAACTCAAATCAGTTAGACCCTTTAATGGTACGCATAGAAGAATTAATCAAGGGAAAAAAATTTCTCCTCGTCCTAGATGATGTGTGGAACCCAAATTCTAGTCAATGGAATGAATTAATCAAACCCTTGCTTAAGGGTGCCACTGGCAGTAGAATATTGGTAACCACACGAAAAGAGGAGGTTGCTCAAGTAATGGGAGCGACCAATCAAATGACTCACTTGAAGGCATTAAGTGAAGAGTGCACTCGGTTGCTGTTCAATCACATTACCTTCCTTGATAGGGAAGATGAGTTTGAAAAGTTTGAAATGTTTGTTCAAGAGTTTGTAGAGAAGTGTAAAGGTTTACCTCTTGTTGCGAAGACTTTAGGTAGTCTCATGCGCCATAAGAAAACAAAAAAAGAATGGCAAGATGTATTAAAAAGTCCCATATGGGAATTAAAAAGATTTCCCGAGGAGGTTTTTCAACCATTATTGTTAAGTTATTATGATTTGCCTCCAACCATAAGGCGTTGTCTTTTGTATTGCGTTACTTTTCCGAAAGATTATGTGATTGATAAAGATGTTTTGATTGAGTTGTGGATGTCACAAAACTATTTGTACAAGAAGCAAAAGAAAGAAGAAGAGGTTGGTCAAACATATTTTGAGGATTTAGCAACGAGGTCTTTCTTCCAAGATTTTGTGAGAGATGATGATGGGAATGTTACAAAATGCAAAATGCACGATATTATGCATGACCTTTTGCAGTACTTGACCGTGGAAGAGTGTTTTAGTATGGAGGTGAAGGGTGCTGATGAGATGATGGAGCTTCCCAACGGTAACAGTTTTCGTCACTTAACCCTGATGTTTGCATCTCAGGGTTTATCTAGCATCCTTTCTTCTCGAAATTGTGGAAATTTGCGTACCTTCACTACTTTTGCTTCAACAATTTCTAGTATTGAGCCAGAGTTTATAACTTCAGTGAAACGTGTTAGGTCTTTAAATTTGAGTAGTACTGCCCTCAGAGTAGTTCCAAAAGAGGTTGGTGAGTTGCTACATTTGAGATATCTTGATCTATCAAATAATTCCTTTAGGGAATTACCGGACAAAATAGGTGATTTGTACAATCTACAAACCTTGCGGCTTGTTGAGTGCCGTAATCTGGTAAAAGGGTTTGGTATGAGGAAGTTAATCAATTTGAAGCATCTTTACGTTAAGGGTTCTCCGGGTGTAAAGTTAAAAGGTATTAGTAGATTGAAATGGCTGCAAACACTAGATGAGTTTCACGTGAGCGAAGAGACCAGAAAAGAAGGATTGCAGTTGGAGGATCTGGAAAACTTGGAACAGCTTCAAGGGGCTCTTTCCGTGCAACACGTGGCATCGTCATTGCAAACTGTGAGTGGGGCCGTGAGAGCACGTTTGAATAAGAAACGCCTCCTTCACTTGGAACTTAATTTCGACTGTTACGCAGCACCGACGCTCGAAACCGAAGGAGCGATACTGAATGCTTTAAAACCACATCCAGATTTGGAATCATTAAGCATCCTTCACTGTTATGGGGCTACCTCGACTCCCACTTGGCTCATGTCTTTACCAAACTTGAAGAGGCTTACTCTTTTCATCAAGAAATTTGAGGTTTTACCTGCTTTGGGGACACTGTCGTTCCTTGAATCGCTGGATATAAAGATGAATGATAGACTGCAAAAGGTGGGGGTTGTGTTTCTGGGGATAAATCGAATATCATCGTCATCGTCATCGGAGGTTAGTTCATTCCCAAAGTTAAAACACCTCACATTCAGTTTCATCTATAAATGGGAAGAGTGGGAAGGAGTGGATTCTCAGAATATCAGGATAATGCCATGCCTTTCATCCTTAGAAGTGAGGAAATGTGAAAAACTAAAAGCTTTGCCGGAGTTCCTATGGAAGACACCACTGAAGAGTTTGACCATTGTGGATAGTCCAATTCTGCATTCTGTGTACTATGGAGGCAACCATCGTCAGTGGACCACTTATGGTGCGGAACCAGCCCCGATTAATGAGGGGTTTAACAAGATATCTCACATCCCAAACATCAAAATTGGTTATGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ATP_bind_1

Protein Analysis

991

Amino Acids

113.05

Weight (kDa)

6.7

Isoelectric Point (pI)

45.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 6 - 103 4.9e-17 Rx N-terminal domain
NB-ARC PF00931 235 - 394 1.2e-33 NB-ARC domain
AAA_22 PF13401 240 - 357 9.2e-06 AAA domain
WHD_DRP PF23559 481 - 549 5.6e-24 Disease resistance protein Winged helix domain
LRR_14 PF23598 602 - 923 5.5e-29 Leucine-rich repeat region
LRR_8 PF13855 625 - 680 4e-07 Leucine rich repeat
LRR_R13L1-DRL21 PF25019 736 - 857 3.9e-19 R13L1/DRL21 LRRs
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 570, 1859
AasI GACNNNNNNGTC 1 cut(s) 509
Acc36I ACCTGC 1 cut(s) 2536
AccB1I GGYRCC 1 cut(s) 1046
AccII CGCG 1 cut(s) 501
AccIII TCCGGA 1 cut(s) 349
AciI CCGC 5 cut(s) 118, 305, 509, 2026, 2903
AclWI GGATC 2 cut(s) 39, 2220
AcsI RAATTY 5 cut(s) 968, 1003, 1801, 1888, 2513
AcuI CTGAAG 4 cut(s) 95, 131, 1848, 2840
AcvI CACGTG 2 cut(s) 2173, 2260
AfaI GTAC 9 cut(s) 394, 678, 939, 1508, 1655, 1810, 1900, 2009, 2864
AfiI CCNNNNNNNGG 6 cut(s) 740, 934, 1189, 2047, 2329, 2534
AflII CTTAAG 2 cut(s) 287, 1040
AflIII ACRYGT 4 cut(s) 166, 258, 1873, 2257
AhlI ACTAGT 1 cut(s) 778
AjiI CACGTC 1 cut(s) 259
AjnI CCWGG 1 cut(s) 27
AjuI GAANNNNNNNTTGG 4 cut(s) 1518, 1550, 2839, 2871
AluBI AGCT 6 cut(s) 73, 79, 140, 1714, 2233, 2789
AluI AGCT 6 cut(s) 73, 79, 140, 1714, 2233, 2789
Alw21I GWGCWC 3 cut(s) 278, 1154, 2302
Alw26I GTCTC 3 cut(s) 841, 1286, 2177
Alw44I GTGCAC 1 cut(s) 1150
AlwI GGATC 2 cut(s) 39, 2220
Ama87I CYCGRG 1 cut(s) 1358
Aor13HI TCCGGA 1 cut(s) 349
AoxI GGCC 5 cut(s) 177, 201, 336, 804, 2289
ApaLI GTGCAC 1 cut(s) 1150
ApeKI GCWGC 4 cut(s) 292, 438, 2149, 2354
ApoI RAATTY 5 cut(s) 968, 1003, 1801, 1888, 2513
ArsI GACNNNNNNTTYG 2 cut(s) 629, 661
AseI ATTAAT 3 cut(s) 953, 1025, 2919
Asp700I GAANNNNTTC 1 cut(s) 2232
AspLEI GCGC 1 cut(s) 1290
AspS9I GGNCC 6 cut(s) 201, 667, 719, 805, 2289, 2889
AsuC2I CCSGG 1 cut(s) 2112
AsuHPI GGTGA 5 cut(s) 40, 94, 1699, 1943, 2012
AvaI CYCGRG 1 cut(s) 1358
AvaII GGWCC 3 cut(s) 667, 719, 2889
BaeGI GKGCMC 1 cut(s) 1154
BanI GGYRCC 1 cut(s) 1046
BanII GRGCYC 1 cut(s) 2245
BarI GAAGNNNNNNTAC 4 cut(s) 2078, 2110, 2367, 2399
BbrPI CACGTG 2 cut(s) 2173, 2260
BbsI GAAGAC 2 cut(s) 1274, 2816
Bbv12I GWGCWC 3 cut(s) 278, 1154, 2302
BbvI GCAGC 4 cut(s) 304, 425, 2136, 2366
BccI CCATC 7 cut(s) 79, 244, 489, 881, 1595, 1702, 2886
BceAI ACGGC 2 cut(s) 2025, 2276
BciT130I CCWGG 1 cut(s) 29
BcnI CCSGG 1 cut(s) 2112
BcoDI GTCTC 3 cut(s) 841, 1286, 2177
BcuI ACTAGT 1 cut(s) 778
BfaI CTAG 8 cut(s) 527, 689, 779, 983, 1008, 1773, 1839, 2159
BfrI CTTAAG 2 cut(s) 287, 1040
BfuAI ACCTGC 1 cut(s) 2536
BisI GCNGC 5 cut(s) 293, 439, 2027, 2150, 2355
BlsI GCNGC 5 cut(s) 294, 440, 2028, 2151, 2356
BmcAI AGTACT 3 cut(s) 678, 1655, 1900
Bme1390I CCNGG 2 cut(s) 29, 2112
Bme18I GGWCC 3 cut(s) 667, 719, 2889
BmeT110I CYCGRG 1 cut(s) 1358
BmgBI CACGTC 1 cut(s) 259
BmgT120I GGNCC 6 cut(s) 201, 667, 719, 805, 2289, 2889
BmiI GGNNCC 6 cut(s) 202, 720, 998, 1048, 2290, 2907
BmrFI CCNGG 2 cut(s) 29, 2112
BmsI GCATC 6 cut(s) 142, 1766, 1785, 2098, 2273, 2436
BpiI GAAGAC 2 cut(s) 1274, 2816
BplI GAGNNNNNCTC 6 cut(s) 706, 738, 889, 921, 2482, 2514
BpuEI CTTGAG 2 cut(s) 665, 1080
BpuMI CCSGG 1 cut(s) 2112
BsaAI YACGTR 2 cut(s) 2173, 2260
BsaI GGTCTC 1 cut(s) 2177
BsaJI CCNNGG 3 cut(s) 339, 1359, 1662
BsaWI WCCGGW 2 cut(s) 349, 1987
BsaXI ACNNNNNCTCC 2 cut(s) 2440, 2470
Bsc4I CCNNNNNNNGG 6 cut(s) 740, 934, 1189, 2047, 2329, 2534
Bse1I ACTGG 4 cut(s) 221, 306, 467, 1057
Bse3DI GCAATG 2 cut(s) 504, 2270
BseAI TCCGGA 1 cut(s) 349
BseBI CCWGG 1 cut(s) 29
BseDI CCNNGG 3 cut(s) 339, 1359, 1662
BseLI CCNNNNNNNGG 6 cut(s) 740, 934, 1189, 2047, 2329, 2534
BseMI GCAATG 2 cut(s) 504, 2270
BseMII CTCAG 5 cut(s) 557, 852, 1775, 1921, 2738
BseNI ACTGG 4 cut(s) 221, 306, 467, 1057
BseRI GAGGAG 6 cut(s) 173, 378, 727, 965, 1100, 1376
BseSI GKGCMC 1 cut(s) 1154
BseXI GCAGC 4 cut(s) 304, 425, 2136, 2366
Bsh1236I CGCG 1 cut(s) 501
BshFI GGCC 5 cut(s) 179, 203, 338, 806, 2291
BshNI GGYRCC 1 cut(s) 1046
BsiHKAI GWGCWC 3 cut(s) 278, 1154, 2302
BsiHKCI CYCGRG 1 cut(s) 1358
BsiSI CCGG 4 cut(s) 350, 1988, 2111, 2795
BslFI GGGAC 4 cut(s) 100, 225, 1317, 2551
BslI CCNNNNNNNGG 6 cut(s) 740, 934, 1189, 2047, 2329, 2534
BsmAI GTCTC 3 cut(s) 841, 1286, 2177
BsmFI GGGAC 4 cut(s) 100, 225, 1317, 2551
BsmI GAATGC 2 cut(s) 2395, 2854
BsnI GGCC 5 cut(s) 179, 203, 338, 806, 2291
Bso31I GGTCTC 1 cut(s) 2177
BsoBI CYCGRG 1 cut(s) 1358
Bsp1286I GDGCHC 4 cut(s) 278, 1154, 2245, 2302
Bsp13I TCCGGA 1 cut(s) 349
Bsp1407I TGTACA 2 cut(s) 1506, 2007
Bsp143I GATC 4 cut(s) 31, 60, 1957, 2212
Bsp68I TCGCGA 1 cut(s) 501
BspACI CCGC 5 cut(s) 118, 305, 509, 2026, 2903
BspANI GGCC 5 cut(s) 179, 203, 338, 806, 2291
BspCNI CTCAG 5 cut(s) 556, 851, 1774, 1920, 2737
BspEI TCCGGA 1 cut(s) 349
BspFNI CGCG 1 cut(s) 501
BspHI TCATGA 1 cut(s) 244
BspLI GGNNCC 6 cut(s) 202, 720, 998, 1048, 2290, 2907
BspMI ACCTGC 1 cut(s) 2536
BspPI GGATC 2 cut(s) 39, 2220
BspT107I GGYRCC 1 cut(s) 1046
BspTI CTTAAG 2 cut(s) 287, 1040
BspTNI GGTCTC 1 cut(s) 2177
BsrDI GCAATG 2 cut(s) 504, 2270
BsrGI TGTACA 2 cut(s) 1506, 2007
BsrI ACTGG 4 cut(s) 221, 306, 467, 1057
BssECI CCNNGG 3 cut(s) 339, 1359, 1662
BssMI GATC 4 cut(s) 31, 60, 1957, 2212
BssT1I CCWWGG 1 cut(s) 339
Bst2UI CCWGG 1 cut(s) 29
Bst4CI ACNGT 7 cut(s) 1663, 1724, 1730, 2281, 2348, 2438, 2544
Bst6I CTCTTC 9 cut(s) 61, 812, 1140, 1524, 1662, 2175, 2483, 2697, 2816
BstAFI CTTAAG 2 cut(s) 287, 1040
BstAUI TGTACA 2 cut(s) 1506, 2007
BstBAI YACGTR 2 cut(s) 2173, 2260
BstC8I GCNNGC 2 cut(s) 181, 205
BstDEI CTNAG 7 cut(s) 396, 543, 838, 1761, 1907, 2724, 2758
BstDSI CCRYGG 1 cut(s) 1662
BstEII GGTNACC 2 cut(s) 46, 1069
BstENI CCTNNNNNAGG 1 cut(s) 738
BstFNI CGCG 1 cut(s) 501
BstHHI GCGC 1 cut(s) 1290
BstKTI GATC 4 cut(s) 34, 63, 1960, 2215
BstMAI GTCTC 3 cut(s) 841, 1286, 2177
BstMBI GATC 4 cut(s) 31, 60, 1957, 2212
BstMWI GCNNNNNNNGC 3 cut(s) 435, 2297, 2909
BstNI CCWGG 1 cut(s) 29
BstNSI RCATGY 1 cut(s) 170
BstPI GGTNACC 2 cut(s) 46, 1069
BstSCI CCNGG 2 cut(s) 27, 2110
BstSLI GKGCMC 1 cut(s) 1154
BstUI CGCG 1 cut(s) 501
BstV1I GCAGC 4 cut(s) 304, 425, 2136, 2366
BstV2I GAAGAC 2 cut(s) 1274, 2816
BstX2I RGATCY 1 cut(s) 2212
BstYI RGATCY 1 cut(s) 2212
BsuRI GGCC 5 cut(s) 179, 203, 338, 806, 2291
BtgI CCRYGG 1 cut(s) 1662
BtrI CACGTC 1 cut(s) 259
BtsIMutI CAGTG 8 cut(s) 51, 214, 1050, 1872, 2434, 2540, 2816, 2891
BtuMI TCGCGA 1 cut(s) 501
BveI ACCTGC 1 cut(s) 2536
Cac8I GCNNGC 2 cut(s) 181, 205
CciI TCATGA 1 cut(s) 244
CfoI GCGC 1 cut(s) 1290
Cfr13I GGNCC 6 cut(s) 201, 667, 719, 805, 2289, 2889
CseI GACGC 2 cut(s) 452, 2371
Csp6I GTAC 9 cut(s) 393, 677, 938, 1507, 1654, 1809, 1899, 2008, 2863
CviAII CATG 7 cut(s) 167, 184, 245, 1285, 1640, 2470, 2745
CviQI GTAC 9 cut(s) 393, 677, 938, 1507, 1654, 1809, 1899, 2008, 2863
DdeI CTNAG 7 cut(s) 396, 543, 838, 1761, 1907, 2724, 2758
DpnI GATC 4 cut(s) 33, 62, 1959, 2214
DpnII GATC 4 cut(s) 31, 60, 1957, 2212
DraI TTTAAA 2 cut(s) 1887, 2397
DrdI GACNNNNNNGTC 1 cut(s) 509
DseDI GACNNNNNNGTC 1 cut(s) 509
Eam1104I CTCTTC 9 cut(s) 61, 812, 1140, 1524, 1662, 2175, 2483, 2697, 2816
EarI CTCTTC 9 cut(s) 61, 812, 1140, 1524, 1662, 2175, 2483, 2697, 2816
EciI GGCGGA 1 cut(s) 320
Eco130I CCWWGG 1 cut(s) 339
Eco147I AGGCCT 1 cut(s) 338
Eco24I GRGCYC 1 cut(s) 2245
Eco31I GGTCTC 1 cut(s) 2177
Eco32I GATATC 2 cut(s) 1952, 2940
Eco47I GGWCC 3 cut(s) 667, 719, 2889
Eco57I CTGAAG 4 cut(s) 95, 131, 1848, 2840
Eco72I CACGTG 2 cut(s) 2173, 2260
Eco88I CYCGRG 1 cut(s) 1358
Eco91I GGTNACC 2 cut(s) 46, 1069
EcoNI CCTNNNNNAGG 1 cut(s) 738
EcoO109I RGGNCCY 2 cut(s) 667, 719
EcoO65I GGTNACC 2 cut(s) 46, 1069
EcoRII CCWGG 1 cut(s) 27
EcoRV GATATC 2 cut(s) 1952, 2940
EcoT14I CCWWGG 1 cut(s) 339
EcoT22I ATGCAT 1 cut(s) 1641
EcoT38I GRGCYC 1 cut(s) 2245
ErhI CCWWGG 1 cut(s) 339
FaeI CATG 7 cut(s) 170, 187, 248, 1288, 1643, 2473, 2748
FalI AAGNNNNNCTT 2 cut(s) 2229, 2261
FaqI GGGAC 4 cut(s) 100, 225, 1317, 2551
FatI CATG 7 cut(s) 166, 183, 244, 1284, 1639, 2469, 2744
FauI CCCGC 1 cut(s) 125
FauNDI CATATG 1 cut(s) 1337
Fnu4HI GCNGC 5 cut(s) 293, 439, 2027, 2150, 2355
FriOI GRGCYC 1 cut(s) 2245
Fsp4HI GCNGC 5 cut(s) 293, 439, 2027, 2150, 2355
FspBI CTAG 8 cut(s) 527, 689, 779, 983, 1008, 1773, 1839, 2159
GlaI GCGC 1 cut(s) 1289
GluI GCNGC 5 cut(s) 293, 439, 2027, 2150, 2355
HaeIII GGCC 5 cut(s) 179, 203, 338, 806, 2291
HapII CCGG 4 cut(s) 350, 1988, 2111, 2795
HgaI GACGC 2 cut(s) 452, 2371
HhaI GCGC 1 cut(s) 1290
Hin1II CATG 7 cut(s) 170, 187, 248, 1288, 1643, 2473, 2748
Hin6I GCGC 1 cut(s) 1288
HinP1I GCGC 1 cut(s) 1288
HincII GTYRAC 2 cut(s) 540, 776
HindII GTYRAC 2 cut(s) 540, 776
HindIII AAGCTT 1 cut(s) 2787
HinfI GANTC 8 cut(s) 283, 346, 444, 1123, 2417, 2455, 2555, 2720
HpaI GTTAAC 1 cut(s) 540
HpaII CCGG 4 cut(s) 350, 1988, 2111, 2795
HphI GGTGA 5 cut(s) 40, 94, 1699, 1943, 2012
Hpy166II GTNNAC 6 cut(s) 540, 776, 1152, 1253, 2863, 2889
Hpy188I TCNGA 8 cut(s) 65, 137, 546, 841, 1446, 1910, 2644, 2727
Hpy8I GTNNAC 6 cut(s) 540, 776, 1152, 1253, 2863, 2889
Hpy99I CGWCG 3 cut(s) 260, 501, 2365
HpyCH4III ACNGT 7 cut(s) 1663, 1724, 1730, 2281, 2348, 2438, 2544
HpyCH4IV ACGT 7 cut(s) 258, 523, 1873, 2097, 2172, 2259, 2302
HpyF10VI GCNNNNNNNGC 3 cut(s) 435, 2297, 2909
HpyF3I CTNAG 7 cut(s) 396, 543, 838, 1761, 1907, 2724, 2758
HpySE526I ACGT 7 cut(s) 258, 523, 1873, 2097, 2172, 2259, 2302
Hsp92II CATG 7 cut(s) 170, 187, 248, 1288, 1643, 2473, 2748
HspAI GCGC 1 cut(s) 1288
Kpn2I TCCGGA 1 cut(s) 349
KspAI GTTAAC 1 cut(s) 540
Kzo9I GATC 4 cut(s) 31, 60, 1957, 2212
LmnI GCTCC 5 cut(s) 273, 391, 1106, 1711, 2378
Lsp1109I GCAGC 4 cut(s) 304, 425, 2136, 2366
LweI GCATC 6 cut(s) 142, 1766, 1785, 2098, 2273, 2436
MaeI CTAG 8 cut(s) 527, 689, 779, 983, 1008, 1773, 1839, 2159
MaeII ACGT 7 cut(s) 258, 523, 1873, 2097, 2172, 2259, 2302
MaeIII GTNAC 9 cut(s) 46, 193, 1069, 1435, 1491, 1609, 1724, 1736, 2348
MalI GATC 4 cut(s) 33, 62, 1959, 2214
MboI GATC 4 cut(s) 31, 60, 1957, 2212
MflI RGATCY 1 cut(s) 2212
MhlI GDGCHC 4 cut(s) 278, 1154, 2245, 2302
MlyI GAGTC 3 cut(s) 453, 1117, 2449
MmeI TCCRAC 3 cut(s) 243, 1430, 2187
Mph1103I ATGCAT 1 cut(s) 1641
MroI TCCGGA 1 cut(s) 349
MroXI GAANNNNTTC 1 cut(s) 2232
MslI CAYNNNNRTG 1 cut(s) 2439
MspCI CTTAAG 2 cut(s) 287, 1040
MspI CCGG 4 cut(s) 350, 1988, 2111, 2795
MspR9I CCNGG 2 cut(s) 29, 2112
Mva1269I GAATGC 2 cut(s) 2395, 2854
MvaI CCWGG 1 cut(s) 29
MvnI CGCG 1 cut(s) 501
MwoI GCNNNNNNNGC 3 cut(s) 435, 2297, 2909
NciI CCSGG 1 cut(s) 2112
NdeI CATATG 1 cut(s) 1337
NdeII GATC 4 cut(s) 31, 60, 1957, 2212
NlaIII CATG 7 cut(s) 170, 187, 248, 1288, 1643, 2473, 2748
NlaIV GGNNCC 6 cut(s) 202, 720, 998, 1048, 2290, 2907
NmeAIII GCCGAG 1 cut(s) 467
NmuCI GTSAC 4 cut(s) 46, 193, 1491, 1736
NruI TCGCGA 1 cut(s) 501
NsiI ATGCAT 1 cut(s) 1641
NspI RCATGY 1 cut(s) 170
PagI TCATGA 1 cut(s) 244
PceI AGGCCT 1 cut(s) 338
PciI ACATGT 1 cut(s) 166
PctI GAATGC 2 cut(s) 2395, 2854
PdmI GAANNNNTTC 1 cut(s) 2232
PfeI GAWTC 5 cut(s) 283, 346, 2417, 2555, 2720
PflFI GACNNNGTC 2 cut(s) 797, 2542
PfoI TCCNGGA 1 cut(s) 27
PkrI GCNGC 5 cut(s) 294, 440, 2028, 2151, 2356
PleI GAGTC 3 cut(s) 452, 1117, 2449
PmaCI CACGTG 2 cut(s) 2173, 2260
PmlI CACGTG 2 cut(s) 2173, 2260
PpsI GAGTC 3 cut(s) 452, 1117, 2449
Ppu21I YACGTR 2 cut(s) 2173, 2260
PpuMI RGGWCCY 2 cut(s) 667, 719
PscI ACATGT 1 cut(s) 166
PshBI ATTAAT 3 cut(s) 953, 1025, 2919
PsiI TTATAA 2 cut(s) 570, 1859
Psp5II RGGWCCY 2 cut(s) 667, 719
Psp6I CCWGG 1 cut(s) 27
PspCI CACGTG 2 cut(s) 2173, 2260
PspEI GGTNACC 2 cut(s) 46, 1069
PspGI CCWGG 1 cut(s) 27
PspN4I GGNNCC 6 cut(s) 202, 720, 998, 1048, 2290, 2907
PspPI GGNCC 6 cut(s) 201, 667, 719, 805, 2289, 2889
PspPPI RGGWCCY 2 cut(s) 667, 719
PsuI RGATCY 1 cut(s) 2212
PsyI GACNNNGTC 2 cut(s) 797, 2542
RruI TCGCGA 1 cut(s) 501
RsaI GTAC 9 cut(s) 394, 678, 939, 1508, 1655, 1810, 1900, 2009, 2864
RsaNI GTAC 9 cut(s) 393, 677, 938, 1507, 1654, 1809, 1899, 2008, 2863
RseI CAYNNNNRTG 1 cut(s) 2439
SatI GCNGC 5 cut(s) 293, 439, 2027, 2150, 2355
Sau3AI GATC 4 cut(s) 31, 60, 1957, 2212
Sau96I GGNCC 6 cut(s) 201, 667, 719, 805, 2289, 2889
ScaI AGTACT 3 cut(s) 678, 1655, 1900
SchI GAGTC 3 cut(s) 453, 1117, 2449
ScrFI CCNGG 2 cut(s) 29, 2112
SduI GDGCHC 4 cut(s) 278, 1154, 2245, 2302
SfaNI GCATC 6 cut(s) 142, 1766, 1785, 2098, 2273, 2436
SinI GGWCC 3 cut(s) 667, 719, 2889
SmiMI CAYNNNNRTG 1 cut(s) 2439
SmlI CTYRAG 4 cut(s) 287, 680, 1040, 1095
SmoI CTYRAG 4 cut(s) 287, 680, 1040, 1095
SpeI ACTAGT 1 cut(s) 778
SseBI AGGCCT 1 cut(s) 338
SsiI CCGC 5 cut(s) 118, 305, 509, 2026, 2903
SspI AATATT 1 cut(s) 1065
SspMI CTAG 8 cut(s) 527, 689, 779, 983, 1008, 1773, 1839, 2159
StuI AGGCCT 1 cut(s) 338
StyD4I CCNGG 2 cut(s) 27, 2110
StyI CCWWGG 1 cut(s) 339
TaaI ACNGT 7 cut(s) 1663, 1724, 1730, 2281, 2348, 2438, 2544
TaiI ACGT 7 cut(s) 261, 526, 1876, 2100, 2175, 2262, 2305
TaqI TCGA 7 cut(s) 174, 652, 1789, 2343, 2367, 2453, 2620
TaqII GACCGA 1 cut(s) 34
TatI WGTACW 6 cut(s) 676, 1506, 1653, 1898, 2007, 2862
TauI GCSGC 1 cut(s) 2029
TfiI GAWTC 5 cut(s) 283, 346, 2417, 2555, 2720
TscAI CASTG 8 cut(s) 58, 221, 1057, 1872, 2441, 2547, 2823, 2891
TseFI GTSAC 4 cut(s) 46, 193, 1491, 1736
TseI GCWGC 4 cut(s) 292, 438, 2149, 2354
Tsp45I GTSAC 4 cut(s) 46, 193, 1491, 1736
TspGWI ACGGA 2 cut(s) 347, 2239
TspRI CASTG 8 cut(s) 58, 221, 1057, 1872, 2441, 2547, 2823, 2891
Tth111I GACNNNGTC 2 cut(s) 797, 2542
Vha464I CTTAAG 2 cut(s) 287, 1040
VneI GTGCAC 1 cut(s) 1150
VpaK11BI GGWCC 3 cut(s) 667, 719, 2889
VspI ATTAAT 3 cut(s) 953, 1025, 2919
XagI CCTNNNNNAGG 1 cut(s) 738
XapI RAATTY 5 cut(s) 968, 1003, 1801, 1888, 2513
XbaI TCTAGA 1 cut(s) 526
XceI RCATGY 1 cut(s) 170
XmnI GAANNNNTTC 1 cut(s) 2232
XspI CTAG 8 cut(s) 527, 689, 779, 983, 1008, 1773, 1839, 2159
ZrmI AGTACT 3 cut(s) 678, 1655, 1900
Zsp2I ATGCAT 1 cut(s) 1641
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.