Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb6

Orthogroup ID

OG0004780

Physical Location & Seq

Genomic Coordinates Reverse (-)

31206962 .. 31212736

Gene Structure 5775 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_6g39513.t3

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2601 bp

ATGATATTCTCTGATGATCATCAGTTAAAATTAAATAGTCTCACCATGGACATAGACCTTCGGCTACCTTCTGGTGACCATGATAAGGAGGATGAAGAGCCAAATGCAATTGATAACATGCTGGAACATGAAGAAAAGATGCACAATGGAGATATTGAGAGTGCACATATTGTGGATGATAGGGATGAGGTACATGCTGAAGATGGGGGAGATTTGAATTCTCCCACAGCAGATATGGTAATCTTCAATGAGGACACAAACCTTGAGCCGCTTTTTGGGATGGAATTTGAATCACATGGTGAAGCATACTCTTTCTATCAGGAGTATGCTCGCTCTATGGGATTCAACACTGCCATCCAAAACAGCCGCCGCTCAAAGACATCAAGAGAATTCATTGATGCAAAGTTTGCTTGTTCCAGATATGGTACCAAGCGTGAGTATGACAAAACCTATAATAATAAGCCACGGGCCAGACAGAGCAAGCAAGACCCAGAAAATGCAACTGGTCGGAGATCATGTTCAAAGACAGACTGCAAAGCTAGCATGCATGTGAAGAGAAGATCAGATGGGAAATGGGTTATACATAATTTTGTGAAGGAGCATAATCATGGGCTTTTACCAGCTCAAGCAGTCAGTGAACAAACAAGAAAGATGTATGCTGCAATGGCTAGACAATTTGCTGAATACAAGAATGTTGTTGGTCTGAAAAATGATCCAAAAAATCCTTTTGATAAAGGTCGGAATTTGTCATTAGAGGCAGGAGATTTGAAGATGTTGCTTGAATTTTTTACTCACATGCAGAGTATGAATTCCAACTTCTTCTATGCCATAGATCTGGGAGAAGATCAATGTCTCAAAACTTTATTGTGGGTTGACGGCAAAAGTAGGCATGATTACATCAATTTCAATGATGTAGTGTCATTGGATACCACTTATATTAGAAGCAAATATAAGATGCCTCTTGTTTTATTTGTTGGTGTGAATCAACACTATCAGTTTGTGCTGCTGGGATGTGCCTTGGTCTCAGATGAATCTGCAACAACATTCTCTTGGCTAATGCAGACATGGCTGAAAGCTATGGGTGGACAAGCTCCAAAAGTCATTATCTCTGATCATGACCAATCAATAAAATCAGTTGTTTCAGAGGTCTTTCCAAATGCTCATCATTGTTTTTGTTTGTGGAATATTTTGGGGAAGGTTTCAGAAAATCTTGGTCATGTAATTAAACGTCACGAGAATTTTCTGGCGAACTTCGAAGAATGTATTCACAGGCCATCTACAACTGAAGAGTTTGAGAAAAGATGGTGCGACATTGTTGATGAATATGAACTTAAAGATAATGAGTGGACTCAGTCATTGTATGAAGATCGAAAGCATTGGGTACCAACATACATGACAAATGTTTGCTTGGCAGGGATGTCTACAGTTCAGCGATCTGACAGTGTGAACTCCTTTTTTGATAAGTATGTTCATAAGAAGACCACTGTGCAAGAGTTCTTGAAACAGTATGGAGCAATTCTACAAGATAGGTATGAAGAGGAAGCCAAAGCAGATTCTGATATGTGGAACAAACAGCCCACAACAAAATCTCCTTCCCCATTGGAGAAGAGTGTCTCCTTGATATATACACCCTCAGTACTGAAGAAATTTCAAGTGGAGGTTTTGGGTGCAATTGGCTGTAATCCTAAAAGGGATAGACAAGATGAGATGACCACTACCTACAGAGTACATGATTGTGAAAAGAATCAAGATTTCACTGTAGCATGGAATGAAATGAAGTTAGAAGTTTCGTGTTCATGCTGCTTATTTGAATACAGAGGTTATCTTTGTAGACATGCTTTAATTGTTCTCCAAATGTGTCAGATTGGTACAATCCCAGATCAGTATATATTAAAGCGTTGGACAAAAGATGCAAAGAGCAGGCATTTGGTGGTGCAGGAACCTGGCGATGTACCTTCCAGGGTGCAAAAATTCGATGATTTATCTCAGCGCGCTATGAAGTTAATTGGGGAAGGATCTCTGTCACAGGAGAGTTACACTATTGTATGTCATGCACTAGAAGAAGCTTTTGGAAATTGTTTGGCTGTGAATAATTCTAGTAAGAGTCTGGTAGAAGCTGGAACATCAGGCACTCAGGGCCTGCCTTGCATTGAAGATGATATTCAAAATAGGAGTATTGGCAAGGGTAGTAAGAAAAAGAATCCAACCAAGAAAAGAAAGGTGAACTCTGAGCCAGAGGTCATGACTGTTGGAGCACAAGAGAGCTTACAACAAATGGATAAATTGAACTCAAGAGCGATAACCATCGATGGTTATTATGGTGGACAACAGAATGTGCAAGGAATGGTTCAATTGAACTTAATGGCACCTACACGGGACAATTACTACGGGAATCAAGTCAATCAACAGACTATTCAGGGGCTTGGCCAGCTGAATTCTATAGCCCCAAGCCATCCAAGCCATGAAAGTTATTACAGTGCTCAGCAAAGCATGCATGGGCTGGGACAAATGGAATTTTTCCGAACCGGTTTTACTTACATTCGGGATGACCCCAATGTAAGAACAGCTCCATTGCACGATGATGCATCAAGACATGCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003700 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0007154 GO:0007165 GO:0007602 GO:0007623 GO:0008150 GO:0009314 GO:0009416 GO:0009581 GO:0009582 GO:0009583 GO:0009585 GO:0009605 GO:0009628 GO:0009639 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010017 GO:0010018 GO:0010218 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0019219 GO:0019222 GO:0023052 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0042752 GO:0042753 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0045893 GO:0045935 GO:0048511 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0051606 GO:0051716 GO:0060255 GO:0065007 GO:0071214 GO:0071478 GO:0071482 GO:0071489 GO:0071490 GO:0080090 GO:0104004 GO:0140110 GO:1902680 GO:1903506 GO:1903508 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

FAR1 MULE SWIM

Protein Analysis

867

Amino Acids

99.01

Weight (kDa)

5.93

Isoelectric Point (pI)

51.68

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
FAR1	PF03101	89 - 191	4.8e-26	FAR1 DNA-binding domain
ZSWIM1-3_RNaseH-like	PF21056	260 - 373	8.3e-09	Zinc finger SWIM domain-containing protein 1/3, RNaseH-like domain
MULE	PF10551	290 - 382	3.3e-29	MULE transposase domain
SWIM	PF04434	579 - 606	1.7e-07	SWIM zinc finger

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0004780)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G22170 AT3G22170
fragaria_vesca	FvH4_6g39513 FvH4_6g39513 FvH4_6g39513 FvH4_6g39513
malus_domestica	MD09G1139500.v1.1 MD17G1129000.v1.1
prunus_persica	Prupe.3G186200_v2.0.a1 Prupe.3G186200_v2.0.a1 Prupe.3G186200_v2.0.a1 Prupe.3G186200_v2.0.a1
pyrus_communis	pycom07g28200 pycom09g06010 pycom09g06020 pycom09g06050 pycom17g12100 pycom17g12110
rosa_chinensis	RchiOBHm_Chr2g0153571
rosa_laevigata	RLG00000020710
rosa_multiflora	Rmu_ssc0000024.1_g000021
rosa_roxburghii	Rroxscaffold_2G00094910
rosa_rugosa	Rorug02G0440200 Rorug02G0440300 Rorug02G0440400 Rorug02G0440500 Rorug02G0440600
rosa_samantha	Rh2BG514100 Rh2CG489200 Rh2DG525800
rosa_wichuraiana	Rw2G041340

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	2 cut(s)	425, 1381
AccB1I	GGYRCC	3 cut(s)	425, 1381, 2365
AccBSI	CCGCTC	1 cut(s)	372
AccI	GTMKAC	2 cut(s)	1421, 1831
AccII	CGCG	1 cut(s)	1992
AciI	CCGC	3 cut(s)	269, 367, 370
AclWI	GGATC	2 cut(s)	707, 2023
AcoI	YGGCCR	1 cut(s)	2425
AcuI	CTGAAG	3 cut(s)	219, 1305, 1661
AdeI	CACNNNGTG	1 cut(s)	299
AfaI	GTAC	7 cut(s)	192, 427, 1383, 1638, 1728, 1870, 1953
AfiI	CCNNNNNNNGG	2 cut(s)	85, 275
AgeI	ACCGGT	1 cut(s)	2525
AjnI	CCWGG	2 cut(s)	1942, 1958
AjuI	GAANNNNNNNTTGG	2 cut(s)	2052, 2084
AloI	GAACNNNNNNTCC	2 cut(s)	502, 534
AluBI	AGCT	9 cut(s)	539, 623, 1076, 1091, 2066, 2117, 2265, 2431, 2567
AluI	AGCT	9 cut(s)	539, 623, 1076, 1091, 2066, 2117, 2265, 2431, 2567
Alw21I	GWGCWC	3 cut(s)	166, 2257, 2482
Alw26I	GTCTC	4 cut(s)	44, 857, 1027, 1618
Alw44I	GTGCAC	1 cut(s)	162
AlwI	GGATC	2 cut(s)	707, 2023
AlwNI	CAGNNNCTG	2 cut(s)	1556, 2140
AoxI	GGCC	4 cut(s)	468, 1271, 2137, 2425
ApaLI	GTGCAC	1 cut(s)	162
ApeKI	GCWGC	3 cut(s)	659, 1003, 1800
AsiGI	ACCGGT	1 cut(s)	2525
Asp700I	GAANNNNTTC	1 cut(s)	1263
Asp718I	GGTACC	2 cut(s)	425, 1381
AspLEI	GCGC	2 cut(s)	1992, 1994
AspS9I	GGNCC	2 cut(s)	468, 2137
AsuHPI	GGTGA	4 cut(s)	34, 86, 311, 2233
AsuII	TTCGAA	1 cut(s)	1254
AsuNHI	GCTAGC	1 cut(s)	539
BaeGI	GKGCMC	1 cut(s)	166
BalI	TGGCCA	1 cut(s)	2427
BanI	GGYRCC	3 cut(s)	425, 1381, 2365
BauI	CACGAG	1 cut(s)	1232
BbsI	GAAGAC	1 cut(s)	1484
Bbv12I	GWGCWC	3 cut(s)	166, 2257, 2482
BbvI	GCAGC	3 cut(s)	646, 990, 1787
BccI	CCATC	9 cut(s)	197, 274, 362, 560, 1282, 1296, 2303, 2312, 2460
BceAI	ACGGC	1 cut(s)	892
BciT130I	CCWGG	2 cut(s)	1944, 1960
BciVI	GTATCC	1 cut(s)	919
BclI	TGATCA	2 cut(s)	16, 1111
BcoDI	GTCTC	4 cut(s)	44, 857, 1027, 1618
BfaI	CTAG	4 cut(s)	540, 669, 2057, 2097
BfmI	CTRYAG	4 cut(s)	1422, 1720, 1758, 2439
BfuI	GTATCC	1 cut(s)	919
BglII	AGATCT	1 cut(s)	832
BisI	GCNGC	6 cut(s)	269, 367, 370, 660, 1004, 1801
BlpI	GCTNAGC	1 cut(s)	2481
BlsI	GCNGC	6 cut(s)	270, 368, 371, 661, 1005, 1802
BmcAI	AGTACT	1 cut(s)	1638
Bme1390I	CCNGG	2 cut(s)	1944, 1960
BmgT120I	GGNCC	2 cut(s)	468, 2137
BmiI	GGNNCC	4 cut(s)	427, 1383, 1941, 2367
BmrFI	CCNGG	2 cut(s)	1944, 1960
BmsI	GCATC	6 cut(s)	129, 388, 945, 1900, 2572, 2594
BmtI	GCTAGC	1 cut(s)	543
BpiI	GAAGAC	1 cut(s)	1484
Bpu1102I	GCTNAGC	1 cut(s)	2481
Bpu14I	TTCGAA	1 cut(s)	1254
BpuEI	CTTGAG	3 cut(s)	284, 609, 2275
Bsa29I	ATCGAT	1 cut(s)	2307
BsaBI	GATNNNNATC	2 cut(s)	18, 2303
BsaI	GGTCTC	1 cut(s)	1027
BsaJI	CCNNGG	4 cut(s)	45, 464, 1017, 1959
BsaWI	WCCGGW	1 cut(s)	2525
BsaXI	ACNNNNNCTCC	4 cut(s)	502, 532, 1573, 1603
Bsc4I	CCNNNNNNNGG	2 cut(s)	85, 275
Bse118I	RCCGGY	1 cut(s)	2525
Bse1I	ACTGG	1 cut(s)	508
Bse3DI	GCAATG	2 cut(s)	669, 2570
Bse8I	GATNNNNATC	2 cut(s)	18, 2303
BseBI	CCWGG	2 cut(s)	1944, 1960
BseCI	ATCGAT	1 cut(s)	2307
BseDI	CCNNGG	4 cut(s)	45, 464, 1017, 1959
BseGI	GGATG	9 cut(s)	97, 181, 190, 285, 354, 1016, 1422, 2452, 2551
BseJI	GATNNNNATC	2 cut(s)	18, 2303
BseLI	CCNNNNNNNGG	2 cut(s)	85, 275
BseMI	GCAATG	2 cut(s)	669, 2570
BseMII	CTCAG	7 cut(s)	1038, 1364, 1647, 2000, 2147, 2220, 2495
BseNI	ACTGG	1 cut(s)	508
BsePI	GCGCGC	1 cut(s)	1990
BseSI	GKGCMC	1 cut(s)	166
BseXI	GCAGC	3 cut(s)	646, 990, 1787
BseYI	CCCAGC	2 cut(s)	1006, 2500
BsgI	GTGCAG	1 cut(s)	1955
Bsh1236I	CGCG	1 cut(s)	1992
BshFI	GGCC	4 cut(s)	470, 1273, 2139, 2427
BshNI	GGYRCC	3 cut(s)	425, 1381, 2365
BshTI	ACCGGT	1 cut(s)	2525
BshVI	ATCGAT	1 cut(s)	2307
BsiHKAI	GWGCWC	3 cut(s)	166, 2257, 2482
BsiSI	CCGG	1 cut(s)	2526
BslFI	GGGAC	2 cut(s)	2390, 2517
BslI	CCNNNNNNNGG	2 cut(s)	85, 275
BsmAI	GTCTC	4 cut(s)	44, 857, 1027, 1618
BsmFI	GGGAC	2 cut(s)	2390, 2517
BsnI	GGCC	4 cut(s)	470, 1273, 2139, 2427
Bso31I	GGTCTC	1 cut(s)	1027
Bsp119I	TTCGAA	1 cut(s)	1254
Bsp1286I	GDGCHC	3 cut(s)	166, 2257, 2482
Bsp1720I	GCTNAGC	1 cut(s)	2481
Bsp19I	CCATGG	1 cut(s)	45
BspACI	CCGC	3 cut(s)	269, 367, 370
BspANI	GGCC	4 cut(s)	470, 1273, 2139, 2427
BspCNI	CTCAG	7 cut(s)	1037, 1363, 1646, 1999, 2146, 2221, 2494
BspDI	ATCGAT	1 cut(s)	2307
BspFNI	CGCG	1 cut(s)	1992
BspHI	TCATGA	2 cut(s)	1114, 2241
BspLI	GGNNCC	4 cut(s)	427, 1383, 1941, 2367
BspOI	GCTAGC	1 cut(s)	543
BspPI	GGATC	2 cut(s)	707, 2023
BspQI	GCTCTTC	1 cut(s)	90
BspT104I	TTCGAA	1 cut(s)	1254
BspT107I	GGYRCC	3 cut(s)	425, 1381, 2365
BspTNI	GGTCTC	1 cut(s)	1027
BsrBI	CCGCTC	1 cut(s)	372
BsrDI	GCAATG	2 cut(s)	669, 2570
BsrFI	RCCGGY	1 cut(s)	2525
BsrI	ACTGG	1 cut(s)	508
BssAI	RCCGGY	1 cut(s)	2525
BssECI	CCNNGG	4 cut(s)	45, 464, 1017, 1959
BssHII	GCGCGC	1 cut(s)	1990
BssSI	CACGAG	1 cut(s)	1232
BssT1I	CCWWGG	2 cut(s)	45, 1017
Bst2BI	CACGAG	1 cut(s)	1232
Bst2UI	CCWGG	2 cut(s)	1944, 1960
Bst4CI	ACNGT	7 cut(s)	1426, 1442, 1486, 1506, 1759, 2248, 2477
Bst6I	CTCTTC	5 cut(s)	90, 548, 1281, 1530, 1601
BstAPI	GCANNNNNTGC	2 cut(s)	407, 2491
BstBI	TTCGAA	1 cut(s)	1254
BstC8I	GCNNGC	9 cut(s)	331, 482, 541, 545, 1922, 1992, 2141, 2429, 2492
BstDEI	CTNAG	7 cut(s)	1024, 1350, 1633, 1986, 2133, 2229, 2481
BstDSI	CCRYGG	2 cut(s)	45, 464
BstEII	GGTNACC	1 cut(s)	74
BstF5I	GGATG	9 cut(s)	97, 181, 190, 285, 354, 1016, 1422, 2452, 2551
BstFNI	CGCG	1 cut(s)	1992
BstHHI	GCGC	2 cut(s)	1992, 1994
BstMAI	GTCTC	4 cut(s)	44, 857, 1027, 1618
BstMWI	GCNNNNNNNGC	9 cut(s)	407, 540, 665, 1066, 2136, 2145, 2428, 2457, 2491
BstNI	CCWGG	2 cut(s)	1944, 1960
BstNSI	RCATGY	8 cut(s)	121, 197, 547, 551, 799, 1838, 2494, 2597
BstPI	GGTNACC	1 cut(s)	74
BstSCI	CCNGG	2 cut(s)	1942, 1958
BstSFI	CTRYAG	4 cut(s)	1422, 1720, 1758, 2439
BstSLI	GKGCMC	1 cut(s)	166
BstUI	CGCG	1 cut(s)	1992
BstV1I	GCAGC	3 cut(s)	646, 990, 1787
BstV2I	GAAGAC	1 cut(s)	1484
BstX2I	RGATCY	2 cut(s)	832, 2015
BstXI	CCANNNNNNTGG	1 cut(s)	835
BstYI	RGATCY	2 cut(s)	832, 2015
Bsu15I	ATCGAT	1 cut(s)	2307
BsuI	GTATCC	1 cut(s)	919
BsuRI	GGCC	4 cut(s)	470, 1273, 2139, 2427
BsuTUI	ATCGAT	1 cut(s)	2307
BtgI	CCRYGG	2 cut(s)	45, 464
BtgZI	GCGATG	1 cut(s)	1962
BtsCI	GGATG	9 cut(s)	97, 181, 190, 285, 354, 1016, 1422, 2452, 2551
BtsI	GCAGTG	1 cut(s)	348
BtsIMutI	CAGTG	6 cut(s)	348, 640, 1447, 1482, 1755, 2482
Cac8I	GCNNGC	9 cut(s)	331, 482, 541, 545, 1922, 1992, 2141, 2429, 2492
CaiI	CAGNNNCTG	2 cut(s)	1556, 2140
CciI	TCATGA	2 cut(s)	1114, 2241
CfoI	GCGC	2 cut(s)	1992, 1994
Cfr10I	RCCGGY	1 cut(s)	2525
Cfr13I	GGNCC	2 cut(s)	468, 2137
ClaI	ATCGAT	1 cut(s)	2307
Csp6I	GTAC	7 cut(s)	191, 426, 1382, 1637, 1727, 1869, 1952
CspAI	ACCGGT	1 cut(s)	2525
CviQI	GTAC	7 cut(s)	191, 426, 1382, 1637, 1727, 1869, 1952
DdeI	CTNAG	7 cut(s)	1024, 1350, 1633, 1986, 2133, 2229, 2481
DraIII	CACNNNGTG	1 cut(s)	299
EaeI	YGGCCR	1 cut(s)	2425
Eam1104I	CTCTTC	5 cut(s)	90, 548, 1281, 1530, 1601
EarI	CTCTTC	5 cut(s)	90, 548, 1281, 1530, 1601
Eco130I	CCWWGG	2 cut(s)	45, 1017
Eco31I	GGTCTC	1 cut(s)	1027
Eco57I	CTGAAG	3 cut(s)	219, 1305, 1661
Eco91I	GGTNACC	1 cut(s)	74
EcoO109I	RGGNCCY	1 cut(s)	2137
EcoO65I	GGTNACC	1 cut(s)	74
EcoRI	GAATTC	4 cut(s)	217, 389, 808, 2434
EcoRII	CCWGG	2 cut(s)	1942, 1958
EcoT14I	CCWWGG	2 cut(s)	45, 1017
EcoT22I	ATGCAT	3 cut(s)	549, 2496, 2587
ErhI	CCWWGG	2 cut(s)	45, 1017
FalI	AAGNNNNNCTT	1 cut(s)	2581
FaqI	GGGAC	2 cut(s)	2390, 2517
FbaI	TGATCA	2 cut(s)	16, 1111
FblI	GTMKAC	2 cut(s)	1421, 1831
Fnu4HI	GCNGC	6 cut(s)	269, 367, 370, 660, 1004, 1801
FokI	GGATG	9 cut(s)	104, 188, 197, 292, 341, 1023, 1429, 2439, 2558
Fsp4HI	GCNGC	6 cut(s)	269, 367, 370, 660, 1004, 1801
FspBI	CTAG	4 cut(s)	540, 669, 2057, 2097
GlaI	GCGC	2 cut(s)	1991, 1993
GluI	GCNGC	6 cut(s)	269, 367, 370, 660, 1004, 1801
GsaI	CCCAGC	2 cut(s)	1010, 2504
HaeIII	GGCC	4 cut(s)	470, 1273, 2139, 2427
HapII	CCGG	1 cut(s)	2526
HhaI	GCGC	2 cut(s)	1992, 1994
Hin6I	GCGC	2 cut(s)	1990, 1992
HinP1I	GCGC	2 cut(s)	1990, 1992
HincII	GTYRAC	1 cut(s)	874
HindII	GTYRAC	1 cut(s)	874
HindIII	AAGCTT	1 cut(s)	2064
HpaII	CCGG	1 cut(s)	2526
HphI	GGTGA	4 cut(s)	34, 86, 311, 2233
HpyAV	CCTTC	7 cut(s)	68, 78, 589, 1189, 1602, 1965, 2006
HpyCH4III	ACNGT	7 cut(s)	1426, 1442, 1486, 1506, 1759, 2248, 2477
HpyCH4IV	ACGT	1 cut(s)	1228
HpyF10VI	GCNNNNNNNGC	9 cut(s)	407, 540, 665, 1066, 2136, 2145, 2428, 2457, 2491
HpyF3I	CTNAG	7 cut(s)	1024, 1350, 1633, 1986, 2133, 2229, 2481
HpySE526I	ACGT	1 cut(s)	1228
HspAI	GCGC	2 cut(s)	1990, 1992
KpnI	GGTACC	2 cut(s)	429, 1385
Ksp22I	TGATCA	2 cut(s)	16, 1111
LguI	GCTCTTC	1 cut(s)	90
LmnI	GCTCC	5 cut(s)	598, 1096, 1511, 2252, 2572
Lsp1109I	GCAGC	3 cut(s)	646, 990, 1787
LweI	GCATC	6 cut(s)	129, 388, 945, 1900, 2572, 2594
MaeI	CTAG	4 cut(s)	540, 669, 2057, 2097
MaeII	ACGT	1 cut(s)	1228
MaeIII	GTNAC	4 cut(s)	74, 1229, 2022, 2033
MbiI	CCGCTC	1 cut(s)	372
MfeI	CAATTG	3 cut(s)	108, 1671, 2351
MflI	RGATCY	2 cut(s)	832, 2015
MhlI	GDGCHC	3 cut(s)	166, 2257, 2482
MlsI	TGGCCA	1 cut(s)	2427
MluNI	TGGCCA	1 cut(s)	2427
MlyI	GAGTC	2 cut(s)	1342, 2113
MmeI	TCCRAC	6 cut(s)	488, 719, 837, 1880, 2228, 2230
Mox20I	TGGCCA	1 cut(s)	2427
Mph1103I	ATGCAT	3 cut(s)	549, 2496, 2587
MroXI	GAANNNNTTC	1 cut(s)	1263
MscI	TGGCCA	1 cut(s)	2427
MseI	TTAA	8 cut(s)	26, 32, 1224, 1332, 1841, 1892, 2003, 2360
MslI	CAYNNNNRTG	4 cut(s)	548, 606, 1734, 2580
Msp20I	TGGCCA	1 cut(s)	2427
MspA1I	CMGCKG	1 cut(s)	2431
MspI	CCGG	1 cut(s)	2526
MspR9I	CCNGG	2 cut(s)	1944, 1960
MunI	CAATTG	3 cut(s)	108, 1671, 2351
MvaI	CCWGG	2 cut(s)	1944, 1960
MvnI	CGCG	1 cut(s)	1992
MwoI	GCNNNNNNNGC	9 cut(s)	407, 540, 665, 1066, 2136, 2145, 2428, 2457, 2491
NcoI	CCATGG	1 cut(s)	45
NheI	GCTAGC	1 cut(s)	539
NlaIV	GGNNCC	4 cut(s)	427, 1383, 1941, 2367
NmuCI	GTSAC	3 cut(s)	74, 1229, 2022
NsiI	ATGCAT	3 cut(s)	549, 2496, 2587
NspI	RCATGY	8 cut(s)	121, 197, 547, 551, 799, 1838, 2494, 2597
NspV	TTCGAA	1 cut(s)	1254
PaeI	GCATGC	2 cut(s)	547, 2494
PagI	TCATGA	2 cut(s)	1114, 2241
PauI	GCGCGC	1 cut(s)	1990
PciSI	GCTCTTC	1 cut(s)	90
PdmI	GAANNNNTTC	1 cut(s)	1263
PfeI	GAWTC	8 cut(s)	290, 342, 982, 1031, 1553, 1744, 2200, 2392
PflFI	GACNNNGTC	1 cut(s)	1351
PinAI	ACCGGT	1 cut(s)	2525
PkrI	GCNGC	6 cut(s)	270, 368, 371, 661, 1005, 1802
PleI	GAGTC	2 cut(s)	1342, 2112
PpsI	GAGTC	2 cut(s)	1342, 2112
Psp6I	CCWGG	2 cut(s)	1942, 1958
PspEI	GGTNACC	1 cut(s)	74
PspFI	CCCAGC	2 cut(s)	1006, 2500
PspGI	CCWGG	2 cut(s)	1942, 1958
PspN4I	GGNNCC	4 cut(s)	427, 1383, 1941, 2367
PspPI	GGNCC	2 cut(s)	468, 2137
PsrI	GAACNNNNNNTAC	2 cut(s)	2515, 2547
PstNI	CAGNNNCTG	2 cut(s)	1556, 2140
PsuI	RGATCY	2 cut(s)	832, 2015
PsyI	GACNNNGTC	1 cut(s)	1351
PteI	GCGCGC	1 cut(s)	1990
PvuII	CAGCTG	1 cut(s)	2431
RsaI	GTAC	7 cut(s)	192, 427, 1383, 1638, 1728, 1870, 1953
RsaNI	GTAC	7 cut(s)	191, 426, 1382, 1637, 1727, 1869, 1952
RseI	CAYNNNNRTG	4 cut(s)	548, 606, 1734, 2580
SapI	GCTCTTC	1 cut(s)	90
SaqAI	TTAA	8 cut(s)	26, 32, 1224, 1332, 1841, 1892, 2003, 2360
SatI	GCNGC	6 cut(s)	269, 367, 370, 660, 1004, 1801
Sau96I	GGNCC	2 cut(s)	468, 2137
ScaI	AGTACT	1 cut(s)	1638
SchI	GAGTC	2 cut(s)	1342, 2113
ScrFI	CCNGG	2 cut(s)	1944, 1960
SduI	GDGCHC	3 cut(s)	166, 2257, 2482
SfaNI	GCATC	6 cut(s)	129, 388, 945, 1900, 2572, 2594
SfcI	CTRYAG	4 cut(s)	1422, 1720, 1758, 2439
SfuI	TTCGAA	1 cut(s)	1254
SmiMI	CAYNNNNRTG	4 cut(s)	548, 606, 1734, 2580
SmlI	CTYRAG	3 cut(s)	263, 624, 2290
SmoI	CTYRAG	3 cut(s)	263, 624, 2290
SphI	GCATGC	2 cut(s)	547, 2494
SsiI	CCGC	3 cut(s)	269, 367, 370
SspI	AATATT	1 cut(s)	1186
SspMI	CTAG	4 cut(s)	540, 669, 2057, 2097
StyD4I	CCNGG	2 cut(s)	1942, 1958
StyI	CCWWGG	2 cut(s)	45, 1017
TaaI	ACNGT	7 cut(s)	1426, 1442, 1486, 1506, 1759, 2248, 2477
TaiI	ACGT	1 cut(s)	1231
TaqI	TCGA	4 cut(s)	1254, 1369, 1974, 2307
TatI	WGTACW	2 cut(s)	1636, 1726
TauI	GCSGC	3 cut(s)	271, 369, 372
TfiI	GAWTC	8 cut(s)	290, 342, 982, 1031, 1553, 1744, 2200, 2392
Tru1I	TTAA	8 cut(s)	26, 32, 1224, 1332, 1841, 1892, 2003, 2360
Tru9I	TTAA	8 cut(s)	26, 32, 1224, 1332, 1841, 1892, 2003, 2360
TscAI	CASTG	6 cut(s)	355, 640, 1447, 1489, 1762, 2482
TseFI	GTSAC	3 cut(s)	74, 1229, 2022
TseI	GCWGC	3 cut(s)	659, 1003, 1800
Tsp45I	GTSAC	3 cut(s)	74, 1229, 2022
TspRI	CASTG	6 cut(s)	355, 640, 1447, 1489, 1762, 2482
Tth111I	GACNNNGTC	1 cut(s)	1351
VneI	GTGCAC	1 cut(s)	162
XceI	RCATGY	8 cut(s)	121, 197, 547, 551, 799, 1838, 2494, 2597
XcmI	CCANNNNNNNNNTGG	1 cut(s)	232
XmiI	GTMKAC	2 cut(s)	1421, 1831
XmnI	GAANNNNTTC	1 cut(s)	1263
XspI	CTAG	4 cut(s)	540, 669, 2057, 2097
ZrmI	AGTACT	1 cut(s)	1638
Zsp2I	ATGCAT	3 cut(s)	549, 2496, 2587

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.