Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr17

Orthogroup ID

OG0004780

Physical Location & Seq

Genomic Coordinates Forward (+)

11339296 .. 11345111

Gene Structure 5816 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD17G1129000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2592 bp

ATGATGTTTTCCGATAGCAACCAGTTAAAATTAAATAACCTCACCATGGACATTGATCTACGGCTACCTTCTGGTGAGCATGACAAAGAAGATGAAGAGCCTCATGAAATTGATAACATGTTGGAACATGAAGAAAAAGTGCACAACGGAGATATAGAGAATGGAAACATTGAGGATGTTGGAGATGAAGTACTTGCTGAAGATGGCGGGGATTTGAATTCTCCTACACCAGATATTGTAGTGTTTAAAGAGGATACAAATCTTGAGCCGCTTTTTGGTATGGAGTTTGAATCACATGGGGAAGCATACTCTTTTTATCAGGAGTATGCTCGGTCTATGGGGTTCAACACCGCAATCCAAAACAGCCGCCGTTCAAAGACATCGAGAGAATTTATTGATGCGAAATTTGCTTGTTCTAGATATGGAACCAAGCGCGAGTATGACAAGTCGTATAATAGACCACGTGCCAGACAGAACAAGCAAGACCCAGAAAATCCGACTGGTCGGCGTTCATGTTCAAAGACAGACTGCAAAGCTTCGATGCATGTGAAGAGACGACCAGATGGTAAATGGGTTATACATAATTTTGTGAAGGAGCATAACCACGAGCTTTTACCAGCCCAAGCAGTCAGTGAACAGACAAGAAAAATGTACGCTGCAATGGCTAGACAATTTGCTGAATACAAAAATGTGGTTGGTCTAAAAAGTGATTCCAAAAATCCTTTTGATAAGGGTAGGAATTTGGCATTAGAGGCAGGAGATTTGAAGATTTTGCTCGAGTTTTTCACACAGATGCAGAATATGAATTCCAACTTCTTCTATTCTATAGATCTGGGAGAAGACCAACGGCTGAAGAGTTTGTTTTGGGTTGATGCTAAAAGTAGGCATGACTACATCAATTTCAGTGATATAGTGTCTTTTGATACTACCTACATTAGGAACAAATATAAAATGCCTCTTGCTTTATTTGTTGGAGTGAATCAACACTATCAGTTCGTGCTGCTAGGATGTGCTTTGGTATCAGATGAAAGTACAGCAACTTTTTCTTGGCTAATGCAGACGTGGTTGAAAGCTATGGGTGGACAAGCTCCAAAAGTAATAATCACTGACCATGACAAGTCGATAAAATCAGTTATCGCAGAGGTCTTTCCAAGTGCTTATCATTGTTTTTCTTTGTGGCATATTTTGGGGAAGGTTTCTGAAAATCTTGGCCATGTGATTAAACAGCATGAAAATTTTATGGCGAAATTTGAAAAGTGCATCCACAGGTCATCTACAAATGAAGAGTTTGAAAAAAGATGGTGGAAAATTGTTGAGAAATTTGAACTGAAAGAGGATGAATGGACTCAGTTGTTGTATGAAGATCGAAAACAGTGGGTACCAATGTACATGAGGGAAATTTGTTTGGCTGGGATGTCTGCAGTTCAGCGATCTGAGAGTGTGAACTCCTTTTTTGACAAGTATGTGCATAAGAAGACCACTGTTCAAGAATTCCTGAAACAGTACGAAGCAATTCTACAAGATAGGTATGATGAGGAAGCCAAAGCAGATTATGATACATGGAACAAACCTCCAACATTGAGATCTCCTTCGCCCTTGGAGAAGAGCGTCTCAGGGCTGTACACACATGCAGTATTCAAGAAAATTCAAGGTGAGGTTTTAGGTGCAGTTGCTTGTCACCCTAAACGGGAAAGACAAGATGAGACAAGCATTATTTTCAACGTGGTAGATTTTGAAAAGAATCAAGATTTCATCGTAACATGGAATGAAATGAAGACGGAGGTTTCTTGTGTATGCTGCTTATTTGAATATAAAGGTTATCTTTGTAGACATGCATTGATTGTTCTCCAAATATGTGGCCTATCTGCAATCCCATCACAATATGTATTAAAACGTTGGACAAAAGATGCAAAGAGCCAGCATTTTGTGGGAGAAGAATCCGACATTGTACTATCCAGGGTGCAAAAGTTTAATGATTTATGTCAACGGGCAATGAAAGTGATTGAGGAAGGAACTCTATCTCAGGAGAGTTACAGTGTTGCATGCCGTGCACTAGAAGAAGCTTTTGGAAATTGTGTGAGTGTAAATAATTCTAGTAAGAGTCTGTTAGAAGCTAGCACATCAGTCACTCATGGCCTGCTTTGCATTGAAGATGATAACCAAAATAGAAGTATGAGCAGCAAGACAAACAAGAAAAAGAATCCGACGAAGAAAAGAAAGGTGAACTGTGAGCCGGAGGTTATGACTGTTGGTGCACAAGACGGCTTGCAACAAATGGAGAAATTGACCTCAAGAACAGTAACCCTTGATGGCTATTATGGTGCCCAACCGAATGTGCAAGGAATGGTACAATTGAATCTAATGGCGCCTACACGGGATAACTACTATGGGAATCAACAGACCATTCAGGGGCTTGGACAACTGAACTCTATAGCCCCAAGTCATGACGGTTATTACAGTGCTCAGCAAAGCATGCATGGGCTGGGACAGATGGATTTTTTCAGAACTCCAACTGGTTTTGCTTATGGAATTCGGGATGACCCCAATGTAAGAACCACTCCATTGCATGAGGATGCATCCAGACATGCTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003700 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0007154 GO:0007165 GO:0007602 GO:0007623 GO:0008150 GO:0009314 GO:0009416 GO:0009581 GO:0009582 GO:0009583 GO:0009585 GO:0009605 GO:0009628 GO:0009639 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010017 GO:0010018 GO:0010218 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0019219 GO:0019222 GO:0023052 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0042752 GO:0042753 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0045893 GO:0045935 GO:0048511 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0051606 GO:0051716 GO:0060255 GO:0065007 GO:0071214 GO:0071478 GO:0071482 GO:0071489 GO:0071490 GO:0080090 GO:0104004 GO:0140110 GO:1902680 GO:1903506 GO:1903508 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

FAR1 MULE SWIM

Protein Analysis

864

Amino Acids

99.11

Weight (kDa)

6.02

Isoelectric Point (pI)

48.62

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
FAR1	PF03101	104 - 206	1.6e-26	FAR1 DNA-binding domain
ZSWIM1-3_RNaseH-like	PF21056	270 - 386	1.1e-09	Zinc finger SWIM domain-containing protein 1/3, RNaseH-like domain
MULE	PF10551	305 - 396	2.3e-29	MULE transposase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004780)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G22170 AT3G22170
fragaria_vesca	FvH4_6g39513 FvH4_6g39513 FvH4_6g39513 FvH4_6g39513
malus_domestica	MD09G1139500.v1.1 MD17G1129000.v1.1
prunus_persica	Prupe.3G186200_v2.0.a1 Prupe.3G186200_v2.0.a1 Prupe.3G186200_v2.0.a1 Prupe.3G186200_v2.0.a1
pyrus_communis	pycom07g28200 pycom09g06010 pycom09g06020 pycom09g06050 pycom17g12100 pycom17g12110
rosa_chinensis	RchiOBHm_Chr2g0153571
rosa_laevigata	RLG00000020710
rosa_multiflora	Rmu_ssc0000024.1_g000021
rosa_roxburghii	Rroxscaffold_2G00094910
rosa_rugosa	Rorug02G0440200 Rorug02G0440300 Rorug02G0440400 Rorug02G0440500 Rorug02G0440600
rosa_samantha	Rh2BG514100 Rh2CG489200 Rh2DG525800
rosa_wichuraiana	Rw2G041340

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	1378
AccB1I	GGYRCC	3 cut(s)	1378, 2321, 2365
AccI	GTMKAC	1 cut(s)	1828
AccII	CGCG	1 cut(s)	435
AciI	CCGC	4 cut(s)	207, 269, 351, 367
AclI	AACGTT	1 cut(s)	1894
AcoI	YGGCCR	1 cut(s)	1210
AcuI	CTGAAG	2 cut(s)	219, 872
AcvI	CACGTG	1 cut(s)	464
AcyI	GRCGYC	1 cut(s)	2366
AfaI	GTAC	9 cut(s)	192, 653, 1033, 1380, 1388, 1505, 1622, 1950, 2349
AfiI	CCNNNNNNNGG	5 cut(s)	46, 275, 936, 1687, 2409
AflIII	ACRYGT	1 cut(s)	117
AjiI	CACGTC	1 cut(s)	1062
AjnI	CCWGG	1 cut(s)	1955
AjuI	GAANNNNNNNTTGG	2 cut(s)	2049, 2081
AluBI	AGCT	6 cut(s)	536, 610, 1073, 1088, 2063, 2114
AluI	AGCT	6 cut(s)	536, 610, 1073, 1088, 2063, 2114
Alw21I	GWGCWC	4 cut(s)	144, 2053, 2257, 2464
Alw26I	GTCTC	3 cut(s)	547, 1615, 1697
Alw44I	GTGCAC	3 cut(s)	140, 2049, 2253
Ama87I	CYCGRG	1 cut(s)	776
AoxI	GGCC	3 cut(s)	1210, 1858, 2134
ApaLI	GTGCAC	3 cut(s)	140, 2049, 2253
ApeKI	GCWGC	4 cut(s)	656, 1000, 1797, 2178
Asp700I	GAANNNNTTC	1 cut(s)	1512
Asp718I	GGTACC	1 cut(s)	1378
AspLEI	GCGC	2 cut(s)	435, 2368
AsuHPI	GGTGA	5 cut(s)	34, 86, 1664, 1670, 2233
AsuNHI	GCTAGC	1 cut(s)	2114
AvaI	CYCGRG	1 cut(s)	776
BaeGI	GKGCMC	4 cut(s)	144, 2053, 2257, 2326
BaeI	ACNNNNGTAYC	2 cut(s)	1370, 1403
BalI	TGGCCA	1 cut(s)	1212
BanI	GGYRCC	3 cut(s)	1378, 2321, 2365
BarI	GAAGNNNNNNTAC	2 cut(s)	1500, 1532
BauI	CACGAG	1 cut(s)	605
BbrPI	CACGTG	1 cut(s)	464
BbsI	GAAGAC	3 cut(s)	846, 1481, 1781
Bbv12I	GWGCWC	4 cut(s)	144, 2053, 2257, 2464
BbvI	GCAGC	4 cut(s)	643, 987, 1784, 2190
BccI	CCATC	6 cut(s)	197, 557, 1293, 1882, 2303, 2485
BceAI	ACGGC	5 cut(s)	77, 354, 863, 2031, 2278
BciT130I	CCWGG	1 cut(s)	1957
BciVI	GTATCC	1 cut(s)	247
BcoDI	GTCTC	3 cut(s)	547, 1615, 1697
BfaI	CTAG	6 cut(s)	417, 666, 1004, 2054, 2094, 2115
BfmI	CTRYAG	3 cut(s)	825, 1419, 2430
BfoI	RGCGCY	1 cut(s)	2369
BfuI	GTATCC	1 cut(s)	247
BglII	AGATCT	2 cut(s)	829, 1583
BisI	GCNGC	6 cut(s)	269, 367, 657, 1001, 1798, 2179
BlpI	GCTNAGC	1 cut(s)	2463
BlsI	GCNGC	6 cut(s)	270, 368, 658, 1002, 1799, 2180
BmcAI	AGTACT	1 cut(s)	192
Bme1390I	CCNGG	1 cut(s)	1957
BmeT110I	CYCGRG	1 cut(s)	776
BmgBI	CACGTC	1 cut(s)	1062
BmiI	GGNNCC	4 cut(s)	427, 1380, 2323, 2367
BmrFI	CCNGG	1 cut(s)	1957
BmsI	GCATC	8 cut(s)	388, 531, 783, 862, 1269, 1897, 2563, 2585
BmtI	GCTAGC	1 cut(s)	2118
BpiI	GAAGAC	3 cut(s)	846, 1481, 1781
Bpu1102I	GCTNAGC	1 cut(s)	2463
BpuEI	CTTGAG	2 cut(s)	284, 2275
BsaAI	YACGTR	1 cut(s)	464
BsaBI	GATNNNNATC	1 cut(s)	258
BsaHI	GRCGYC	1 cut(s)	2366
BsaJI	CCNNGG	3 cut(s)	45, 1596, 1956
BsaXI	ACNNNNNCTCC	2 cut(s)	174, 204
Bsc4I	CCNNNNNNNGG	5 cut(s)	46, 275, 936, 1687, 2409
Bse1I	ACTGG	3 cut(s)	22, 505, 2518
Bse3DI	GCAATG	3 cut(s)	666, 1998, 2561
Bse8I	GATNNNNATC	1 cut(s)	258
BseBI	CCWGG	1 cut(s)	1957
BseDI	CCNNGG	3 cut(s)	45, 1596, 1956
BseGI	GGATG	8 cut(s)	181, 1013, 1260, 1342, 1419, 2542, 2576, 2578
BseJI	GATNNNNATC	1 cut(s)	258
BseLI	CCNNNNNNNGG	5 cut(s)	46, 275, 936, 1687, 2409
BseMI	GCAATG	3 cut(s)	666, 1998, 2561
BseMII	CTCAG	5 cut(s)	1361, 1425, 1626, 2036, 2477
BseNI	ACTGG	3 cut(s)	22, 505, 2518
BseSI	GKGCMC	4 cut(s)	144, 2053, 2257, 2326
BseXI	GCAGC	4 cut(s)	643, 987, 1784, 2190
BseYI	CCCAGC	2 cut(s)	1409, 2482
BsgI	GTGCAG	1 cut(s)	1686
Bsh1236I	CGCG	1 cut(s)	435
BshFI	GGCC	3 cut(s)	1212, 1860, 2136
BshNI	GGYRCC	3 cut(s)	1378, 2321, 2365
BsiHKAI	GWGCWC	4 cut(s)	144, 2053, 2257, 2464
BsiHKCI	CYCGRG	1 cut(s)	776
BsiSI	CCGG	1 cut(s)	2234
BslFI	GGGAC	1 cut(s)	2499
BslI	CCNNNNNNNGG	5 cut(s)	46, 275, 936, 1687, 2409
BsmAI	GTCTC	3 cut(s)	547, 1615, 1697
BsmBI	CGTCTC	2 cut(s)	547, 1615
BsmFI	GGGAC	1 cut(s)	2499
BsnI	GGCC	3 cut(s)	1212, 1860, 2136
BsoBI	CYCGRG	1 cut(s)	776
Bsp1286I	GDGCHC	5 cut(s)	144, 2053, 2257, 2326, 2464
Bsp1407I	TGTACA	2 cut(s)	1386, 1620
Bsp143I	GATC	5 cut(s)	55, 829, 1363, 1430, 1583
Bsp1720I	GCTNAGC	1 cut(s)	2463
Bsp19I	CCATGG	1 cut(s)	45
BspACI	CCGC	4 cut(s)	207, 269, 351, 367
BspANI	GGCC	3 cut(s)	1212, 1860, 2136
BspCNI	CTCAG	5 cut(s)	1360, 1426, 1625, 2035, 2476
BspFNI	CGCG	1 cut(s)	435
BspHI	TCATGA	2 cut(s)	103, 2443
BspLI	GGNNCC	4 cut(s)	427, 1380, 2323, 2367
BspMAI	CTGCAG	1 cut(s)	1423
BspOI	GCTAGC	1 cut(s)	2118
BspQI	GCTCTTC	2 cut(s)	90, 1598
BspT107I	GGYRCC	3 cut(s)	1378, 2321, 2365
BsrDI	GCAATG	3 cut(s)	666, 1998, 2561
BsrGI	TGTACA	2 cut(s)	1386, 1620
BsrI	ACTGG	3 cut(s)	22, 505, 2518
BssECI	CCNNGG	3 cut(s)	45, 1596, 1956
BssMI	GATC	5 cut(s)	55, 829, 1363, 1430, 1583
BssNI	GRCGYC	1 cut(s)	2366
BssSI	CACGAG	1 cut(s)	605
BssT1I	CCWWGG	2 cut(s)	45, 1596
Bst2BI	CACGAG	1 cut(s)	605
Bst2UI	CCWGG	1 cut(s)	1957
Bst4CI	ACNGT	9 cut(s)	1374, 1483, 1503, 2036, 2228, 2248, 2299, 2450, 2459
Bst6I	CTCTTC	5 cut(s)	90, 545, 848, 1278, 1598
BstACI	GRCGYC	1 cut(s)	2366
BstAPI	GCANNNNNTGC	2 cut(s)	2048, 2473
BstAUI	TGTACA	2 cut(s)	1386, 1620
BstBAI	YACGTR	1 cut(s)	464
BstC8I	GCNNGC	6 cut(s)	1919, 2044, 2116, 2138, 2267, 2474
BstDEI	CTNAG	5 cut(s)	1347, 1434, 1612, 2022, 2463
BstDSI	CCRYGG	1 cut(s)	45
BstENI	CCTNNNNNAGG	1 cut(s)	934
BstF5I	GGATG	8 cut(s)	181, 1013, 1260, 1342, 1419, 2542, 2576, 2578
BstFNI	CGCG	1 cut(s)	435
BstH2I	RGCGCY	1 cut(s)	2369
BstHHI	GCGC	2 cut(s)	435, 2368
BstKTI	GATC	5 cut(s)	58, 832, 1366, 1433, 1586
BstMAI	GTCTC	3 cut(s)	547, 1615, 1697
BstMBI	GATC	5 cut(s)	55, 829, 1363, 1430, 1583
BstMWI	GCNNNNNNNGC	6 cut(s)	407, 662, 752, 2048, 2142, 2473
BstNI	CCWGG	1 cut(s)	1957
BstNSI	RCATGY	7 cut(s)	121, 548, 1631, 1835, 2046, 2476, 2588
BstSCI	CCNGG	1 cut(s)	1955
BstSFI	CTRYAG	3 cut(s)	825, 1419, 2430
BstSLI	GKGCMC	4 cut(s)	144, 2053, 2257, 2326
BstUI	CGCG	1 cut(s)	435
BstV1I	GCAGC	4 cut(s)	643, 987, 1784, 2190
BstV2I	GAAGAC	3 cut(s)	846, 1481, 1781
BstX2I	RGATCY	2 cut(s)	829, 1583
BstXI	CCANNNNNNTGG	1 cut(s)	1856
BstYI	RGATCY	2 cut(s)	829, 1583
BsuI	GTATCC	1 cut(s)	247
BsuRI	GGCC	3 cut(s)	1212, 1860, 2136
BtgI	CCRYGG	1 cut(s)	45
BtrI	CACGTC	1 cut(s)	1062
BtsCI	GGATG	8 cut(s)	181, 1013, 1260, 1342, 1419, 2542, 2576, 2578
BtsIMutI	CAGTG	7 cut(s)	637, 910, 1104, 1379, 1479, 2041, 2464
Cac8I	GCNNGC	6 cut(s)	1919, 2044, 2116, 2138, 2267, 2474
CciI	TCATGA	2 cut(s)	103, 2443
CfoI	GCGC	2 cut(s)	435, 2368
CseI	GACGC	1 cut(s)	1597
Csp6I	GTAC	9 cut(s)	191, 652, 1032, 1379, 1387, 1504, 1621, 1949, 2348
CviQI	GTAC	9 cut(s)	191, 652, 1032, 1379, 1387, 1504, 1621, 1949, 2348
DdeI	CTNAG	5 cut(s)	1347, 1434, 1612, 2022, 2463
DinI	GGCGCC	1 cut(s)	2367
DpnI	GATC	5 cut(s)	57, 831, 1365, 1432, 1585
DpnII	GATC	5 cut(s)	55, 829, 1363, 1430, 1583
DraI	TTTAAA	1 cut(s)	247
EaeI	YGGCCR	1 cut(s)	1210
Eam1104I	CTCTTC	5 cut(s)	90, 545, 848, 1278, 1598
EarI	CTCTTC	5 cut(s)	90, 545, 848, 1278, 1598
Eco130I	CCWWGG	2 cut(s)	45, 1596
Eco57I	CTGAAG	2 cut(s)	219, 872
Eco72I	CACGTG	1 cut(s)	464
Eco88I	CYCGRG	1 cut(s)	776
EcoNI	CCTNNNNNAGG	1 cut(s)	934
EcoRI	GAATTC	4 cut(s)	217, 805, 1490, 2529
EcoRII	CCWGG	1 cut(s)	1955
EcoT14I	CCWWGG	2 cut(s)	45, 1596
EcoT22I	ATGCAT	4 cut(s)	546, 1837, 2478, 2578
EgeI	GGCGCC	1 cut(s)	2367
EheI	GGCGCC	1 cut(s)	2367
ErhI	CCWWGG	2 cut(s)	45, 1596
Esp3I	CGTCTC	2 cut(s)	547, 1615
FalI	AAGNNNNNCTT	2 cut(s)	1806, 1838
FaqI	GGGAC	1 cut(s)	2499
FauI	CCCGC	1 cut(s)	200
FblI	GTMKAC	1 cut(s)	1828
Fnu4HI	GCNGC	6 cut(s)	269, 367, 657, 1001, 1798, 2179
FokI	GGATG	8 cut(s)	188, 1020, 1247, 1349, 1426, 2549, 2563, 2585
Fsp4HI	GCNGC	6 cut(s)	269, 367, 657, 1001, 1798, 2179
FspBI	CTAG	6 cut(s)	417, 666, 1004, 2054, 2094, 2115
GlaI	GCGC	2 cut(s)	434, 2367
GluI	GCNGC	6 cut(s)	269, 367, 657, 1001, 1798, 2179
GsaI	CCCAGC	2 cut(s)	1413, 2486
HaeII	RGCGCY	1 cut(s)	2369
HaeIII	GGCC	3 cut(s)	1212, 1860, 2136
HapII	CCGG	1 cut(s)	2234
HgaI	GACGC	1 cut(s)	1597
HhaI	GCGC	2 cut(s)	435, 2368
Hin1I	GRCGYC	1 cut(s)	2366
Hin6I	GCGC	2 cut(s)	433, 2366
HinP1I	GCGC	2 cut(s)	433, 2366
HincII	GTYRAC	1 cut(s)	1985
HindII	GTYRAC	1 cut(s)	1985
HindIII	AAGCTT	2 cut(s)	534, 2061
HpaII	CCGG	1 cut(s)	2234
HphI	GGTGA	5 cut(s)	34, 86, 1664, 1670, 2233
Hpy188I	TCNGA	8 cut(s)	13, 498, 1024, 1201, 1435, 1942, 2205, 2504
Hpy99I	CGWCG	1 cut(s)	2209
HpyAV	CCTTC	5 cut(s)	78, 586, 1186, 1599, 2003
HpyCH4III	ACNGT	9 cut(s)	1374, 1483, 1503, 2036, 2228, 2248, 2299, 2450, 2459
HpyCH4IV	ACGT	4 cut(s)	463, 1061, 1722, 1894
HpyF10VI	GCNNNNNNNGC	6 cut(s)	407, 662, 752, 2048, 2142, 2473
HpyF3I	CTNAG	5 cut(s)	1347, 1434, 1612, 2022, 2463
HpySE526I	ACGT	4 cut(s)	463, 1061, 1722, 1894
Hsp92I	GRCGYC	1 cut(s)	2366
HspAI	GCGC	2 cut(s)	433, 2366
KasI	GGCGCC	1 cut(s)	2365
KpnI	GGTACC	1 cut(s)	1382
Kzo9I	GATC	5 cut(s)	55, 829, 1363, 1430, 1583
LguI	GCTCTTC	2 cut(s)	90, 1598
LmnI	GCTCC	2 cut(s)	595, 1093
Lsp1109I	GCAGC	4 cut(s)	643, 987, 1784, 2190
LweI	GCATC	8 cut(s)	388, 531, 783, 862, 1269, 1897, 2563, 2585
MaeI	CTAG	6 cut(s)	417, 666, 1004, 2054, 2094, 2115
MaeII	ACGT	4 cut(s)	463, 1061, 1722, 1894
MaeIII	GTNAC	5 cut(s)	1676, 1756, 2030, 2125, 2299
MalI	GATC	5 cut(s)	57, 831, 1365, 1432, 1585
MboI	GATC	5 cut(s)	55, 829, 1363, 1430, 1583
MfeI	CAATTG	1 cut(s)	2351
MflI	RGATCY	2 cut(s)	829, 1583
MhlI	GDGCHC	5 cut(s)	144, 2053, 2257, 2326, 2464
MlsI	TGGCCA	1 cut(s)	1212
MluNI	TGGCCA	1 cut(s)	1212
Mly113I	GGCGCC	1 cut(s)	2366
MlyI	GAGTC	2 cut(s)	1339, 2110
Mox20I	TGGCCA	1 cut(s)	1212
Mph1103I	ATGCAT	4 cut(s)	546, 1837, 2478, 2578
MroXI	GAANNNNTTC	1 cut(s)	1512
MscI	TGGCCA	1 cut(s)	1212
MseI	TTAA	7 cut(s)	26, 32, 246, 1221, 1889, 1971, 2590
MslI	CAYNNNNRTG	2 cut(s)	791, 2571
Msp20I	TGGCCA	1 cut(s)	1212
MspI	CCGG	1 cut(s)	2234
MspR9I	CCNGG	1 cut(s)	1957
MunI	CAATTG	1 cut(s)	2351
MvaI	CCWGG	1 cut(s)	1957
MvnI	CGCG	1 cut(s)	435
MwoI	GCNNNNNNNGC	6 cut(s)	407, 662, 752, 2048, 2142, 2473
NarI	GGCGCC	1 cut(s)	2366
NcoI	CCATGG	1 cut(s)	45
NdeII	GATC	5 cut(s)	55, 829, 1363, 1430, 1583
NheI	GCTAGC	1 cut(s)	2114
NlaIV	GGNNCC	4 cut(s)	427, 1380, 2323, 2367
NmuCI	GTSAC	2 cut(s)	1676, 2125
NsiI	ATGCAT	4 cut(s)	546, 1837, 2478, 2578
NspI	RCATGY	7 cut(s)	121, 548, 1631, 1835, 2046, 2476, 2588
PaeI	GCATGC	2 cut(s)	2046, 2476
PaeR7I	CTCGAG	1 cut(s)	776
PagI	TCATGA	2 cut(s)	103, 2443
PciI	ACATGT	1 cut(s)	117
PciSI	GCTCTTC	2 cut(s)	90, 1598
PdmI	GAANNNNTTC	1 cut(s)	1512
PfeI	GAWTC	8 cut(s)	290, 710, 979, 1741, 1937, 2200, 2356, 2392
PkrI	GCNGC	6 cut(s)	270, 368, 658, 1002, 1799, 2180
PleI	GAGTC	2 cut(s)	1339, 2109
PluTI	GGCGCC	1 cut(s)	2369
PmaCI	CACGTG	1 cut(s)	464
PmlI	CACGTG	1 cut(s)	464
PpsI	GAGTC	2 cut(s)	1339, 2109
Ppu21I	YACGTR	1 cut(s)	464
PscI	ACATGT	1 cut(s)	117
Psp1406I	AACGTT	1 cut(s)	1894
Psp6I	CCWGG	1 cut(s)	1955
PspCI	CACGTG	1 cut(s)	464
PspFI	CCCAGC	2 cut(s)	1409, 2482
PspGI	CCWGG	1 cut(s)	1955
PspN4I	GGNNCC	4 cut(s)	427, 1380, 2323, 2367
PspXI	VCTCGAGB	1 cut(s)	776
PstI	CTGCAG	1 cut(s)	1423
PsuI	RGATCY	2 cut(s)	829, 1583
RsaI	GTAC	9 cut(s)	192, 653, 1033, 1380, 1388, 1505, 1622, 1950, 2349
RsaNI	GTAC	9 cut(s)	191, 652, 1032, 1379, 1387, 1504, 1621, 1949, 2348
RseI	CAYNNNNRTG	2 cut(s)	791, 2571
SapI	GCTCTTC	2 cut(s)	90, 1598
SaqAI	TTAA	7 cut(s)	26, 32, 246, 1221, 1889, 1971, 2590
SatI	GCNGC	6 cut(s)	269, 367, 657, 1001, 1798, 2179
Sau3AI	GATC	5 cut(s)	55, 829, 1363, 1430, 1583
ScaI	AGTACT	1 cut(s)	192
SchI	GAGTC	2 cut(s)	1339, 2110
ScrFI	CCNGG	1 cut(s)	1957
SduI	GDGCHC	5 cut(s)	144, 2053, 2257, 2326, 2464
SfaNI	GCATC	8 cut(s)	388, 531, 783, 862, 1269, 1897, 2563, 2585
SfcI	CTRYAG	3 cut(s)	825, 1419, 2430
SfoI	GGCGCC	1 cut(s)	2367
Sfr274I	CTCGAG	1 cut(s)	776
SlaI	CTCGAG	1 cut(s)	776
SmiMI	CAYNNNNRTG	2 cut(s)	791, 2571
SmlI	CTYRAG	3 cut(s)	263, 776, 2290
SmoI	CTYRAG	3 cut(s)	263, 776, 2290
SphI	GCATGC	2 cut(s)	2046, 2476
SsiI	CCGC	4 cut(s)	207, 269, 351, 367
SspDI	GGCGCC	1 cut(s)	2365
SspMI	CTAG	6 cut(s)	417, 666, 1004, 2054, 2094, 2115
StyD4I	CCNGG	1 cut(s)	1955
StyI	CCWWGG	2 cut(s)	45, 1596
TaaI	ACNGT	9 cut(s)	1374, 1483, 1503, 2036, 2228, 2248, 2299, 2450, 2459
TaiI	ACGT	4 cut(s)	466, 1064, 1725, 1897
TaqI	TCGA	5 cut(s)	383, 539, 777, 1121, 1366
TaqII	GACCGA	1 cut(s)	321
TatI	WGTACW	5 cut(s)	190, 1031, 1386, 1620, 1948
TauI	GCSGC	2 cut(s)	271, 369
TfiI	GAWTC	8 cut(s)	290, 710, 979, 1741, 1937, 2200, 2356, 2392
Tru1I	TTAA	7 cut(s)	26, 32, 246, 1221, 1889, 1971, 2590
Tru9I	TTAA	7 cut(s)	26, 32, 246, 1221, 1889, 1971, 2590
TscAI	CASTG	7 cut(s)	637, 910, 1111, 1379, 1486, 2041, 2464
TseFI	GTSAC	2 cut(s)	1676, 2125
TseI	GCWGC	4 cut(s)	656, 1000, 1797, 2178
Tsp45I	GTSAC	2 cut(s)	1676, 2125
TspGWI	ACGGA	2 cut(s)	162, 1793
TspRI	CASTG	7 cut(s)	637, 910, 1111, 1379, 1486, 2041, 2464
VneI	GTGCAC	3 cut(s)	140, 2049, 2253
XagI	CCTNNNNNAGG	1 cut(s)	934
XbaI	TCTAGA	1 cut(s)	416
XceI	RCATGY	7 cut(s)	121, 548, 1631, 1835, 2046, 2476, 2588
XcmI	CCANNNNNNNNNTGG	1 cut(s)	567
XhoI	CTCGAG	1 cut(s)	776
XmiI	GTMKAC	1 cut(s)	1828
XmnI	GAANNNNTTC	1 cut(s)	1512
XspI	CTAG	6 cut(s)	417, 666, 1004, 2054, 2094, 2115
ZrmI	AGTACT	1 cut(s)	192
Zsp2I	ATGCAT	4 cut(s)	546, 1837, 2478, 2578

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.