FvH4_7g07320

EF hand family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0005854
Physical Location & Seq
Reverse (-)
7285484 .. 7288806
3323 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g07320.t1

Sequence Viewer

Length: 699 bp
ATGAGTGCAACTATCTCTAATCTCTCTCTGTTGCTCCCAGACAAAGTCAAACACAAAACCCTCCGATATCCAAGATCACACTCTTCACTTGTTCTTCTTCTTCATCACCGAAGCCACCCAAATCGCAGACCCTGTTTCTGTGTCGGTCACCAAAGCCACCCAAATCCCAGACCCTATTTTTGTATCAGTACTCAGCAGTCCGCGATGGGACAAGTCCTCGACAGATTACAAGGTAAGGAATGGAGGCGAAACCAAATCAGGAAGATTACCGATAAGGTGTTTGATCATTTCAAGAATGAGAATGGAACGGCAAATTTGACTTTCGAGGATGTCTACATTGGTGTACTACTTGTATACAATGATATCAACAAGCATTTGCCTGGCCCGCATTTCAATCCGCCCTCTAAAGATTCTATCAGGCTTCTGATTCAGGAACATGATATCAATCTTGATGGAGAAATTAACCGGGAAGAGTTTGCCAAATTGATCAAGCAGATGACTGCCGACACATTTTGTGTTGTCAGTCGGGGACTGATTGTCACATTGGTTGCAGCACCAACAGTTGCAATGGCGACAAAGAGGGCTACTGAGGGTGTACCCCATGTTGGGAAGGTCGTGCAACGGATACCCACTTCAGTTTATGCATCCCTTGTGACTCTTATTGTCGTGTTGGTTCAGCACACACGTAAGGAGTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

233

Amino Acids

26.3

Weight (kDa)

9.72

Isoelectric Point (pI)

33.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7874 PF25284 93 - 195 2.5e-06 Family of unknown function (DUF7874)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 333, 354
AccII CGCG 1 cut(s) 203
AciI CCGC 3 cut(s) 201, 386, 398
AcsI RAATTY 1 cut(s) 313
AcuI CTGAAG 1 cut(s) 618
AfaI GTAC 3 cut(s) 190, 345, 597
AfiI CCNNNNNNNGG 2 cut(s) 605, 606
AflIII ACRYGT 1 cut(s) 683
AgsI TTSAA 2 cut(s) 292, 394
AhdI GACNNNNNGTC 1 cut(s) 536
AjnI CCWGG 1 cut(s) 379
AlwNI CAGNNNCTG 1 cut(s) 132
AoxI GGCC 1 cut(s) 382
ApeKI GCWGC 1 cut(s) 551
ApoI RAATTY 1 cut(s) 313
ArsI GACNNNNNNTTYG 2 cut(s) 162, 194
AspS9I GGNCC 1 cut(s) 383
AsuC2I CCSGG 1 cut(s) 467
AsuHPI GGTGA 2 cut(s) 98, 140
BbvI GCAGC 1 cut(s) 563
BccI CCATC 2 cut(s) 199, 446
BceAI ACGGC 1 cut(s) 324
BciT130I CCWGG 1 cut(s) 381
BciVI GTATCC 1 cut(s) 618
BclI TGATCA 2 cut(s) 283, 486
BcnI CCSGG 1 cut(s) 467
BfuI GTATCC 1 cut(s) 618
BisI GCNGC 1 cut(s) 552
BlsI GCNGC 1 cut(s) 553
BmcAI AGTACT 1 cut(s) 190
Bme1390I CCNGG 2 cut(s) 381, 467
BmeRI GACNNNNNGTC 1 cut(s) 536
BmgT120I GGNCC 1 cut(s) 383
BmrFI CCNGG 2 cut(s) 381, 467
BmsI GCATC 1 cut(s) 653
BpuMI CCSGG 1 cut(s) 467
BsaAI YACGTR 1 cut(s) 686
BsaBI GATNNNNATC 1 cut(s) 444
Bsc4I CCNNNNNNNGG 2 cut(s) 605, 606
Bse3DI GCAATG 1 cut(s) 573
Bse8I GATNNNNATC 1 cut(s) 444
BseBI CCWGG 1 cut(s) 381
BseGI GGATG 2 cut(s) 334, 644
BseJI GATNNNNATC 1 cut(s) 444
BseLI CCNNNNNNNGG 2 cut(s) 605, 606
BseMI GCAATG 1 cut(s) 573
BseMII CTCAG 2 cut(s) 206, 579
BseXI GCAGC 1 cut(s) 563
Bsh1236I CGCG 1 cut(s) 203
BshFI GGCC 1 cut(s) 384
BsiSI CCGG 1 cut(s) 466
BslFI GGGAC 2 cut(s) 222, 543
BslI CCNNNNNNNGG 2 cut(s) 605, 606
BsmFI GGGAC 2 cut(s) 222, 543
BsnI GGCC 1 cut(s) 384
Bsp143I GATC 3 cut(s) 74, 283, 486
BspACI CCGC 3 cut(s) 201, 386, 398
BspANI GGCC 1 cut(s) 384
BspCNI CTCAG 2 cut(s) 205, 580
BspFNI CGCG 1 cut(s) 203
BsrDI GCAATG 1 cut(s) 573
BssMI GATC 3 cut(s) 74, 283, 486
BssNAI GTATAC 1 cut(s) 355
Bst1107I GTATAC 1 cut(s) 355
Bst2UI CCWGG 1 cut(s) 381
Bst4CI ACNGT 1 cut(s) 562
Bst6I CTCTTC 2 cut(s) 88, 465
BstBAI YACGTR 1 cut(s) 686
BstC8I GCNNGC 1 cut(s) 386
BstDEI CTNAG 2 cut(s) 192, 588
BstEII GGTNACC 1 cut(s) 146
BstF5I GGATG 2 cut(s) 334, 644
BstFNI CGCG 1 cut(s) 203
BstKTI GATC 3 cut(s) 77, 286, 489
BstMBI GATC 3 cut(s) 74, 283, 486
BstMWI GCNNNNNNNGC 1 cut(s) 385
BstNI CCWGG 1 cut(s) 381
BstPI GGTNACC 1 cut(s) 146
BstSCI CCNGG 2 cut(s) 379, 465
BstUI CGCG 1 cut(s) 203
BstV1I GCAGC 1 cut(s) 563
BstZ17I GTATAC 1 cut(s) 355
BsuI GTATCC 1 cut(s) 618
BsuRI GGCC 1 cut(s) 384
BtgZI GCGATG 1 cut(s) 218
BtsCI GGATG 2 cut(s) 334, 644
Cac8I GCNNGC 1 cut(s) 386
CaiI CAGNNNCTG 1 cut(s) 132
Cfr13I GGNCC 1 cut(s) 383
Csp6I GTAC 3 cut(s) 189, 344, 596
CviAII CATG 2 cut(s) 437, 602
CviJI RGCY 5 cut(s) 114, 156, 384, 421, 584
CviKI_1 RGCY 5 cut(s) 114, 156, 384, 421, 584
CviQI GTAC 3 cut(s) 189, 344, 596
DdeI CTNAG 2 cut(s) 192, 588
DpnI GATC 3 cut(s) 76, 285, 488
DpnII GATC 3 cut(s) 74, 283, 486
DriI GACNNNNNGTC 1 cut(s) 536
Eam1104I CTCTTC 2 cut(s) 88, 465
Eam1105I GACNNNNNGTC 1 cut(s) 536
EarI CTCTTC 2 cut(s) 88, 465
EciI GGCGGA 1 cut(s) 387
Eco32I GATATC 3 cut(s) 68, 364, 442
Eco57I CTGAAG 1 cut(s) 618
Eco91I GGTNACC 1 cut(s) 146
EcoO65I GGTNACC 1 cut(s) 146
EcoRII CCWGG 1 cut(s) 379
EcoRV GATATC 3 cut(s) 68, 364, 442
EcoT22I ATGCAT 1 cut(s) 646
FaeI CATG 2 cut(s) 440, 605
FaiI YATR 4 cut(s) 355, 438, 603, 642
FaqI GGGAC 2 cut(s) 222, 543
FatI CATG 2 cut(s) 436, 601
FauI CCCGC 1 cut(s) 393
FbaI TGATCA 2 cut(s) 283, 486
FblI GTMKAC 2 cut(s) 333, 354
Fnu4HI GCNGC 1 cut(s) 552
FokI GGATG 2 cut(s) 341, 631
Fsp4HI GCNGC 1 cut(s) 552
GluI GCNGC 1 cut(s) 552
HaeIII GGCC 1 cut(s) 384
HapII CCGG 1 cut(s) 466
Hin1II CATG 2 cut(s) 440, 605
HinfI GANTC 3 cut(s) 410, 427, 655
HpaII CCGG 1 cut(s) 466
HphI GGTGA 2 cut(s) 98, 140
Hpy166II GTNNAC 4 cut(s) 334, 344, 355, 596
Hpy188I TCNGA 2 cut(s) 65, 426
Hpy188III TCNNGA 4 cut(s) 259, 292, 431, 449
Hpy8I GTNNAC 4 cut(s) 334, 344, 355, 596
HpyAV CCTTC 1 cut(s) 604
HpyCH4III ACNGT 1 cut(s) 562
HpyCH4IV ACGT 1 cut(s) 685
HpyCH4V TGCA 5 cut(s) 8, 551, 566, 619, 644
HpyF10VI GCNNNNNNNGC 1 cut(s) 385
HpyF3I CTNAG 2 cut(s) 192, 588
HpySE526I ACGT 1 cut(s) 685
Hsp92II CATG 2 cut(s) 440, 605
Ksp22I TGATCA 2 cut(s) 283, 486
Kzo9I GATC 3 cut(s) 74, 283, 486
LmnI GCTCC 1 cut(s) 39
LpnPI CCDG 9 cut(s) 51, 145, 181, 244, 366, 393, 403, 416, 479
Lsp1109I GCAGC 1 cut(s) 563
LweI GCATC 1 cut(s) 653
MaeII ACGT 1 cut(s) 685
MaeIII GTNAC 3 cut(s) 146, 538, 652
MalI GATC 3 cut(s) 76, 285, 488
MboI GATC 3 cut(s) 74, 283, 486
MboII GAAGA 6 cut(s) 75, 86, 89, 92, 274, 482
MluCI AATT 3 cut(s) 313, 459, 482
MlyI GAGTC 1 cut(s) 649
MnlI CCTC 7 cut(s) 71, 227, 237, 319, 412, 573, 583
Mph1103I ATGCAT 1 cut(s) 646
MseI TTAA 1 cut(s) 462
MspI CCGG 1 cut(s) 466
MspR9I CCNGG 2 cut(s) 381, 467
MvaI CCWGG 1 cut(s) 381
MvnI CGCG 1 cut(s) 203
MwoI GCNNNNNNNGC 1 cut(s) 385
NciI CCSGG 1 cut(s) 467
NdeII GATC 3 cut(s) 74, 283, 486
NlaIII CATG 2 cut(s) 440, 605
NmuCI GTSAC 3 cut(s) 146, 538, 652
NsiI ATGCAT 1 cut(s) 646
PfeI GAWTC 2 cut(s) 410, 427
PflFI GACNNNGTC 1 cut(s) 44
PkrI GCNGC 1 cut(s) 553
PleI GAGTC 1 cut(s) 649
PpsI GAGTC 1 cut(s) 649
Ppu21I YACGTR 1 cut(s) 686
Psp6I CCWGG 1 cut(s) 379
PspEI GGTNACC 1 cut(s) 146
PspGI CCWGG 1 cut(s) 379
PspPI GGNCC 1 cut(s) 383
PstNI CAGNNNCTG 1 cut(s) 132
PsyI GACNNNGTC 1 cut(s) 44
RsaI GTAC 3 cut(s) 190, 345, 597
RsaNI GTAC 3 cut(s) 189, 344, 596
SaqAI TTAA 1 cut(s) 462
SatI GCNGC 1 cut(s) 552
Sau3AI GATC 3 cut(s) 74, 283, 486
Sau96I GGNCC 1 cut(s) 383
ScaI AGTACT 1 cut(s) 190
SchI GAGTC 1 cut(s) 649
ScrFI CCNGG 2 cut(s) 381, 467
SetI ASST 4 cut(s) 235, 279, 615, 688
SfaNI GCATC 1 cut(s) 653
Sse9I AATT 3 cut(s) 313, 459, 482
SsiI CCGC 3 cut(s) 201, 386, 398
StyD4I CCNGG 2 cut(s) 379, 465
TaaI ACNGT 1 cut(s) 562
TaiI ACGT 1 cut(s) 688
TaqI TCGA 2 cut(s) 219, 324
TaqII GACCGA 1 cut(s) 134
TasI AATT 3 cut(s) 313, 459, 482
TatI WGTACW 2 cut(s) 188, 343
TfiI GAWTC 2 cut(s) 410, 427
Tru1I TTAA 1 cut(s) 462
Tru9I TTAA 1 cut(s) 462
TseFI GTSAC 3 cut(s) 146, 538, 652
TseI GCWGC 1 cut(s) 551
Tsp45I GTSAC 3 cut(s) 146, 538, 652
TspDTI ATGAA 1 cut(s) 92
TspGWI ACGGA 1 cut(s) 637
Tth111I GACNNNGTC 1 cut(s) 44
XapI RAATTY 1 cut(s) 313
XmiI GTMKAC 2 cut(s) 333, 354
ZrmI AGTACT 1 cut(s) 190
Zsp2I ATGCAT 1 cut(s) 646
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.