RLG00000029259

EF hand family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0005854
Physical Location & Seq
Reverse (-)
35532589 .. 35536071
3483 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000029259

Sequence Viewer

Length: 654 bp
ATGTCTTTCAGCTTCAGCTTGCCTACTCTACTGTCTATCCATCCCTCACAGACAAAGTCAAACACAAAACCCTCCGATATCCAAGATCAGCTTCTTTTTCTTCGTCATCGGAGCCAGCCACATCACAGAGGCCGTTTTTGTATCAGTCAGTACTCCGCCATGGGACAAGTCCTTGACAAATTACAAGGTAAGCAATGGAGGCGACAGCAAATCAGGAAGATTACGGACAAGGTGTTTGATCATTTCAAAAACAAGGCAGGAAGGGCAAATTTGACATTCGAGGACGTCTACATTGGTGTACTACTTGTGTACAATGATATTAACAAGCGTTTACCTGGCCCACATTTTGATCCACCCTCTAAAGATGCTGTCAGACTACTGATTCAGGAACACGATATCAATCTTGACGGAGAAATTGACCGGGAAGAATTTGCCAAGTTGATGAAGCAGATTACTGCCGACACACTTGTTGTTATCAGTCAGGGAATGATCGTCACATTGGTTGCAGCACCAACAATTGCAATGGCGACAAAGAAGGCTACTGAGGGTGTACCCCATGTTGGGAAGGTCGTGCAACGGTTACCCACTTCAGTTTATGCATCCCTTGTGACTCTTGTTGTCATCATGGTTCAGCATGCATGTCAGGAGCCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

218

Amino Acids

24.49

Weight (kDa)

9.49

Isoelectric Point (pI)

41.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7874 PF25284 80 - 180 2.5e-06 Family of unknown function (DUF7874)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 288
AccI GTMKAC 1 cut(s) 288
AciI CCGC 1 cut(s) 156
AclWI GGATC 1 cut(s) 344
AcsI RAATTY 2 cut(s) 268, 428
AcuI CTGAAG 1 cut(s) 573
AcyI GRCGYC 1 cut(s) 285
AfaI GTAC 4 cut(s) 152, 300, 311, 552
AfiI CCNNNNNNNGG 2 cut(s) 560, 561
AgsI TTSAA 1 cut(s) 247
AjnI CCWGG 1 cut(s) 334
AjuI GAANNNNNNNTTGG 2 cut(s) 75, 107
AluBI AGCT 3 cut(s) 12, 18, 91
AluI AGCT 3 cut(s) 12, 18, 91
AlwI GGATC 1 cut(s) 344
AoxI GGCC 2 cut(s) 130, 337
ApeKI GCWGC 1 cut(s) 506
ApoI RAATTY 2 cut(s) 268, 428
ArsI GACNNNNNNTTYG 2 cut(s) 218, 250
AspS9I GGNCC 1 cut(s) 338
AsuC2I CCSGG 1 cut(s) 422
BanII GRGCYC 1 cut(s) 651
BbvI GCAGC 1 cut(s) 518
BccI CCATC 1 cut(s) 48
BceAI ACGGC 1 cut(s) 117
BciT130I CCWGG 1 cut(s) 336
BclI TGATCA 1 cut(s) 238
BcnI CCSGG 1 cut(s) 422
BisI GCNGC 1 cut(s) 507
BlsI GCNGC 1 cut(s) 508
BmcAI AGTACT 1 cut(s) 152
Bme1390I CCNGG 2 cut(s) 336, 422
BmgT120I GGNCC 1 cut(s) 338
BmiI GGNNCC 2 cut(s) 113, 648
BmrFI CCNGG 2 cut(s) 336, 422
BmsI GCATC 2 cut(s) 355, 608
BpuMI CCSGG 1 cut(s) 422
BsaBI GATNNNNATC 1 cut(s) 399
BsaHI GRCGYC 1 cut(s) 285
BsaJI CCNNGG 1 cut(s) 159
Bsc4I CCNNNNNNNGG 2 cut(s) 560, 561
Bse3DI GCAATG 2 cut(s) 200, 528
Bse8I GATNNNNATC 1 cut(s) 399
BseBI CCWGG 1 cut(s) 336
BseDI CCNNGG 1 cut(s) 159
BseGI GGATG 2 cut(s) 40, 599
BseJI GATNNNNATC 1 cut(s) 399
BseLI CCNNNNNNNGG 2 cut(s) 560, 561
BseMI GCAATG 2 cut(s) 200, 528
BseMII CTCAG 1 cut(s) 534
BseXI GCAGC 1 cut(s) 518
BshFI GGCC 2 cut(s) 132, 339
BsiSI CCGG 1 cut(s) 421
BslFI GGGAC 1 cut(s) 177
BslI CCNNNNNNNGG 2 cut(s) 560, 561
BsmFI GGGAC 1 cut(s) 177
BsnI GGCC 2 cut(s) 132, 339
Bsp1286I GDGCHC 1 cut(s) 651
Bsp1407I TGTACA 1 cut(s) 309
Bsp143I GATC 4 cut(s) 85, 238, 349, 489
Bsp19I CCATGG 1 cut(s) 159
BspACI CCGC 1 cut(s) 156
BspANI GGCC 2 cut(s) 132, 339
BspCNI CTCAG 1 cut(s) 535
BspLI GGNNCC 2 cut(s) 113, 648
BspPI GGATC 1 cut(s) 344
BsrDI GCAATG 2 cut(s) 200, 528
BsrGI TGTACA 1 cut(s) 309
BssECI CCNNGG 1 cut(s) 159
BssMI GATC 4 cut(s) 85, 238, 349, 489
BssNI GRCGYC 1 cut(s) 285
BssT1I CCWWGG 1 cut(s) 159
Bst2UI CCWGG 1 cut(s) 336
Bst4CI ACNGT 2 cut(s) 33, 579
BstACI GRCGYC 1 cut(s) 285
BstAUI TGTACA 1 cut(s) 309
BstC8I GCNNGC 3 cut(s) 20, 116, 636
BstDEI CTNAG 1 cut(s) 543
BstDSI CCRYGG 1 cut(s) 159
BstEII GGTNACC 1 cut(s) 579
BstF5I GGATG 2 cut(s) 40, 599
BstKTI GATC 4 cut(s) 88, 241, 352, 492
BstMBI GATC 4 cut(s) 85, 238, 349, 489
BstMWI GCNNNNNNNGC 2 cut(s) 199, 263
BstNI CCWGG 1 cut(s) 336
BstNSI RCATGY 2 cut(s) 638, 642
BstPI GGTNACC 1 cut(s) 579
BstSCI CCNGG 2 cut(s) 334, 420
BstV1I GCAGC 1 cut(s) 518
BsuRI GGCC 2 cut(s) 132, 339
BtgI CCRYGG 1 cut(s) 159
BtsCI GGATG 2 cut(s) 40, 599
Cac8I GCNNGC 3 cut(s) 20, 116, 636
Cfr13I GGNCC 1 cut(s) 338
Csp6I GTAC 4 cut(s) 151, 299, 310, 551
CviAII CATG 5 cut(s) 160, 557, 625, 635, 639
CviJI RGCY 9 cut(s) 12, 18, 91, 114, 118, 132, 339, 539, 649
CviKI_1 RGCY 9 cut(s) 12, 18, 91, 114, 118, 132, 339, 539, 649
CviQI GTAC 4 cut(s) 151, 299, 310, 551
DdeI CTNAG 1 cut(s) 543
DpnI GATC 4 cut(s) 87, 240, 351, 491
DpnII GATC 4 cut(s) 85, 238, 349, 489
EciI GGCGGA 1 cut(s) 145
Eco130I CCWWGG 1 cut(s) 159
Eco24I GRGCYC 1 cut(s) 651
Eco32I GATATC 2 cut(s) 79, 397
Eco57I CTGAAG 1 cut(s) 573
Eco91I GGTNACC 1 cut(s) 579
EcoO65I GGTNACC 1 cut(s) 579
EcoRII CCWGG 1 cut(s) 334
EcoRV GATATC 2 cut(s) 79, 397
EcoT14I CCWWGG 1 cut(s) 159
EcoT22I ATGCAT 2 cut(s) 601, 640
EcoT38I GRGCYC 1 cut(s) 651
ErhI CCWWGG 1 cut(s) 159
FaeI CATG 5 cut(s) 163, 560, 628, 638, 642
FaiI YATR 6 cut(s) 161, 558, 597, 626, 636, 640
FalI AAGNNNNNCTT 2 cut(s) 75, 107
FaqI GGGAC 1 cut(s) 177
FatI CATG 5 cut(s) 159, 556, 624, 634, 638
FbaI TGATCA 1 cut(s) 238
FblI GTMKAC 1 cut(s) 288
Fnu4HI GCNGC 1 cut(s) 507
FokI GGATG 2 cut(s) 27, 586
FriOI GRGCYC 1 cut(s) 651
Fsp4HI GCNGC 1 cut(s) 507
GluI GCNGC 1 cut(s) 507
HaeIII GGCC 2 cut(s) 132, 339
HapII CCGG 1 cut(s) 421
Hin1I GRCGYC 1 cut(s) 285
Hin1II CATG 5 cut(s) 163, 560, 628, 638, 642
HinfI GANTC 2 cut(s) 382, 610
HpaII CCGG 1 cut(s) 421
Hpy166II GTNNAC 5 cut(s) 289, 299, 310, 332, 551
Hpy188I TCNGA 3 cut(s) 76, 111, 374
Hpy188III TCNNGA 4 cut(s) 214, 386, 404, 644
Hpy8I GTNNAC 5 cut(s) 289, 299, 310, 332, 551
HpyAV CCTTC 3 cut(s) 255, 529, 559
HpyCH4III ACNGT 2 cut(s) 33, 579
HpyCH4IV ACGT 1 cut(s) 285
HpyCH4V TGCA 5 cut(s) 506, 521, 574, 599, 638
HpyF10VI GCNNNNNNNGC 2 cut(s) 199, 263
HpyF3I CTNAG 1 cut(s) 543
HpySE526I ACGT 1 cut(s) 285
Hsp92I GRCGYC 1 cut(s) 285
Hsp92II CATG 5 cut(s) 163, 560, 628, 638, 642
Ksp22I TGATCA 1 cut(s) 238
Kzo9I GATC 4 cut(s) 85, 238, 349, 489
LmnI GCTCC 2 cut(s) 111, 646
LpnPI CCDG 9 cut(s) 128, 199, 243, 321, 348, 371, 434, 467, 629
Lsp1109I GCAGC 1 cut(s) 518
LweI GCATC 2 cut(s) 355, 608
MaeII ACGT 1 cut(s) 285
MaeIII GTNAC 3 cut(s) 493, 579, 607
MalI GATC 4 cut(s) 87, 240, 351, 491
MboI GATC 4 cut(s) 85, 238, 349, 489
MboII GAAGA 3 cut(s) 92, 229, 437
MfeI CAATTG 1 cut(s) 516
MhlI GDGCHC 1 cut(s) 651
MluCI AATT 5 cut(s) 179, 268, 414, 428, 516
MlyI GAGTC 1 cut(s) 604
MnlI CCTC 7 cut(s) 55, 82, 122, 192, 274, 367, 538
Mph1103I ATGCAT 2 cut(s) 601, 640
MseI TTAA 1 cut(s) 321
MspI CCGG 1 cut(s) 421
MspR9I CCNGG 2 cut(s) 336, 422
MunI CAATTG 1 cut(s) 516
MvaI CCWGG 1 cut(s) 336
MwoI GCNNNNNNNGC 2 cut(s) 199, 263
NciI CCSGG 1 cut(s) 422
NcoI CCATGG 1 cut(s) 159
NdeII GATC 4 cut(s) 85, 238, 349, 489
NlaIII CATG 5 cut(s) 163, 560, 628, 638, 642
NlaIV GGNNCC 2 cut(s) 113, 648
NmuCI GTSAC 2 cut(s) 493, 607
NsiI ATGCAT 2 cut(s) 601, 640
NspI RCATGY 2 cut(s) 638, 642
PaeI GCATGC 1 cut(s) 638
PfeI GAWTC 1 cut(s) 382
PflFI GACNNNGTC 1 cut(s) 55
PkrI GCNGC 1 cut(s) 508
PleI GAGTC 1 cut(s) 604
PpsI GAGTC 1 cut(s) 604
Psp6I CCWGG 1 cut(s) 334
PspEI GGTNACC 1 cut(s) 579
PspGI CCWGG 1 cut(s) 334
PspN4I GGNNCC 2 cut(s) 113, 648
PspPI GGNCC 1 cut(s) 338
PsyI GACNNNGTC 1 cut(s) 55
RsaI GTAC 4 cut(s) 152, 300, 311, 552
RsaNI GTAC 4 cut(s) 151, 299, 310, 551
SaqAI TTAA 1 cut(s) 321
SatI GCNGC 1 cut(s) 507
Sau3AI GATC 4 cut(s) 85, 238, 349, 489
Sau96I GGNCC 1 cut(s) 338
ScaI AGTACT 1 cut(s) 152
SchI GAGTC 1 cut(s) 604
ScrFI CCNGG 2 cut(s) 336, 422
SduI GDGCHC 1 cut(s) 651
SetI ASST 8 cut(s) 14, 20, 93, 190, 234, 288, 337, 570
SfaNI GCATC 2 cut(s) 355, 608
SphI GCATGC 1 cut(s) 638
Sse9I AATT 5 cut(s) 179, 268, 414, 428, 516
SsiI CCGC 1 cut(s) 156
StyD4I CCNGG 2 cut(s) 334, 420
StyI CCWWGG 1 cut(s) 159
TaaI ACNGT 2 cut(s) 33, 579
TaiI ACGT 1 cut(s) 288
TaqI TCGA 1 cut(s) 279
TasI AATT 5 cut(s) 179, 268, 414, 428, 516
TatI WGTACW 3 cut(s) 150, 298, 309
TfiI GAWTC 1 cut(s) 382
Tru1I TTAA 1 cut(s) 321
Tru9I TTAA 1 cut(s) 321
TseFI GTSAC 2 cut(s) 493, 607
TseI GCWGC 1 cut(s) 506
Tsp45I GTSAC 2 cut(s) 493, 607
TspDTI ATGAA 1 cut(s) 458
TspGWI ACGGA 2 cut(s) 239, 423
Tth111I GACNNNGTC 1 cut(s) 55
XapI RAATTY 2 cut(s) 268, 428
XceI RCATGY 2 cut(s) 638, 642
XmiI GTMKAC 1 cut(s) 288
ZraI GACGTC 1 cut(s) 286
ZrmI AGTACT 1 cut(s) 152
Zsp2I ATGCAT 2 cut(s) 601, 640
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.