MD02G1236300.v1.1

EF hand family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0005854
Physical Location & Seq
Reverse (-)
28251548 .. 28253030
1483 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1236300.v1.1.491

Sequence Viewer

Length: 528 bp
ATGAAGCAACCGGTAATTACATATATGTTTATAAGGTATCTGGTAAGTTTCTGGATTGCAGGTAAGCAATGGAGGCGAAAGCAAATAAAAAGGATTTCAGAGAAGTTGTTTGAACAATTTAGAAATGATAGAAGAACGGATAACTTGAAAATCAACGATCTGTACATCGGTGTACTACTTGTGTACAATGATATCAATAAGCATTTATCCGGCCAGCATTTTGATCCACCCTCGAAAGATCATGTCAGAGAAATGATGAAGGAAAGCGATTTAAATCTTGATGGAGAAATTGACCGCGAAGAGTTTGCAAAGTTCATCGAGCACTTGACAGCGGACAATTTGGTTGTTCTCGATCAACTGATCCTCACACTGGTTGCAGCACCGGCGGTTGCAATGGCGACAAAGAGGGTTACCGAGGGTGTACCGGGGATCGGAAAGGTCGTGCAAAGGTTACCCGATTCAGTTTATGCATCCCTTGTGACTCTTACTGTTGTGCTGCTTCAACAAGCGCACCAGGAGTTCGGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

176

Amino Acids

20.21

Weight (kDa)

7.96

Isoelectric Point (pI)

46.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7874 PF25284 36 - 169 5.7e-06 Family of unknown function (DUF7874)
EF-hand_7 PF13499 72 - 106 4.5e-06 EF-hand domain pair
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 32
Acc36I ACCTGC 1 cut(s) 50
AccII CGCG 1 cut(s) 297
AciI CCGC 3 cut(s) 295, 332, 386
AclWI GGATC 3 cut(s) 218, 355, 437
AcoI YGGCCR 1 cut(s) 211
AfaI GTAC 4 cut(s) 164, 174, 185, 423
AfiI CCNNNNNNNGG 2 cut(s) 370, 431
AgeI ACCGGT 1 cut(s) 10
AgsI TTSAA 3 cut(s) 113, 148, 503
AjnI CCWGG 1 cut(s) 513
Alw21I GWGCWC 1 cut(s) 324
AlwI GGATC 3 cut(s) 218, 355, 437
AoxI GGCC 1 cut(s) 211
ApeKI GCWGC 2 cut(s) 377, 496
AsiGI ACCGGT 1 cut(s) 10
AspLEI GCGC 1 cut(s) 511
AsuC2I CCSGG 1 cut(s) 426
Bbv12I GWGCWC 1 cut(s) 324
BbvI GCAGC 2 cut(s) 389, 483
BccI CCATC 1 cut(s) 275
BcgI CGANNNNNNTGC 2 cut(s) 287, 321
BciT130I CCWGG 1 cut(s) 515
BcnI CCSGG 1 cut(s) 426
BfuAI ACCTGC 1 cut(s) 50
BisI GCNGC 2 cut(s) 378, 497
BlsI GCNGC 2 cut(s) 379, 498
Bme1390I CCNGG 2 cut(s) 426, 515
BmrFI CCNGG 2 cut(s) 426, 515
BmsI GCATC 1 cut(s) 479
BpuMI CCSGG 1 cut(s) 426
BsaBI GATNNNNATC 1 cut(s) 273
BsaJI CCNNGG 2 cut(s) 414, 425
BsaWI WCCGGW 1 cut(s) 10
Bsc4I CCNNNNNNNGG 2 cut(s) 370, 431
Bse118I RCCGGY 2 cut(s) 10, 382
Bse1I ACTGG 1 cut(s) 375
Bse3DI GCAATG 2 cut(s) 74, 399
Bse8I GATNNNNATC 1 cut(s) 273
BseBI CCWGG 1 cut(s) 515
BseDI CCNNGG 2 cut(s) 414, 425
BseGI GGATG 1 cut(s) 470
BseJI GATNNNNATC 1 cut(s) 273
BseLI CCNNNNNNNGG 2 cut(s) 370, 431
BseMI GCAATG 2 cut(s) 74, 399
BseNI ACTGG 1 cut(s) 375
BseXI GCAGC 2 cut(s) 389, 483
Bsh1236I CGCG 1 cut(s) 297
BshFI GGCC 1 cut(s) 213
BshTI ACCGGT 1 cut(s) 10
BsiHKAI GWGCWC 1 cut(s) 324
BsiSI CCGG 4 cut(s) 11, 210, 383, 425
BslI CCNNNNNNNGG 2 cut(s) 370, 431
BsnI GGCC 1 cut(s) 213
Bsp1286I GDGCHC 1 cut(s) 324
Bsp1407I TGTACA 2 cut(s) 162, 183
Bsp143I GATC 6 cut(s) 157, 223, 238, 352, 360, 429
BspACI CCGC 3 cut(s) 295, 332, 386
BspANI GGCC 1 cut(s) 213
BspFNI CGCG 1 cut(s) 297
BspMI ACCTGC 1 cut(s) 50
BspPI GGATC 3 cut(s) 218, 355, 437
BsrDI GCAATG 2 cut(s) 74, 399
BsrFI RCCGGY 2 cut(s) 10, 382
BsrGI TGTACA 2 cut(s) 162, 183
BsrI ACTGG 1 cut(s) 375
BssAI RCCGGY 2 cut(s) 10, 382
BssECI CCNNGG 2 cut(s) 414, 425
BssMI GATC 6 cut(s) 157, 223, 238, 352, 360, 429
Bst2UI CCWGG 1 cut(s) 515
Bst4CI ACNGT 1 cut(s) 490
Bst6I CTCTTC 1 cut(s) 294
BstAUI TGTACA 2 cut(s) 162, 183
BstC8I GCNNGC 1 cut(s) 215
BstEII GGTNACC 2 cut(s) 409, 450
BstF5I GGATG 1 cut(s) 470
BstFNI CGCG 1 cut(s) 297
BstHHI GCGC 1 cut(s) 511
BstKTI GATC 6 cut(s) 160, 226, 241, 355, 363, 432
BstMBI GATC 6 cut(s) 157, 223, 238, 352, 360, 429
BstMWI GCNNNNNNNGC 2 cut(s) 73, 383
BstNI CCWGG 1 cut(s) 515
BstPI GGTNACC 2 cut(s) 409, 450
BstSCI CCNGG 2 cut(s) 424, 513
BstUI CGCG 1 cut(s) 297
BstV1I GCAGC 2 cut(s) 389, 483
BsuRI GGCC 1 cut(s) 213
BtsCI GGATG 1 cut(s) 470
BtsIMutI CAGTG 1 cut(s) 368
BveI ACCTGC 1 cut(s) 50
Cac8I GCNNGC 1 cut(s) 215
CfoI GCGC 1 cut(s) 511
Cfr10I RCCGGY 2 cut(s) 10, 382
Csp6I GTAC 4 cut(s) 163, 173, 184, 422
CspAI ACCGGT 1 cut(s) 10
CviAII CATG 1 cut(s) 242
CviJI RGCY 1 cut(s) 213
CviKI_1 RGCY 1 cut(s) 213
CviQI GTAC 4 cut(s) 163, 173, 184, 422
DpnI GATC 6 cut(s) 159, 225, 240, 354, 362, 431
DpnII GATC 6 cut(s) 157, 223, 238, 352, 360, 429
DraI TTTAAA 1 cut(s) 273
EaeI YGGCCR 1 cut(s) 211
Eam1104I CTCTTC 1 cut(s) 294
EarI CTCTTC 1 cut(s) 294
Eco32I GATATC 1 cut(s) 193
Eco91I GGTNACC 2 cut(s) 409, 450
EcoO65I GGTNACC 2 cut(s) 409, 450
EcoRII CCWGG 1 cut(s) 513
EcoRV GATATC 1 cut(s) 193
EcoT22I ATGCAT 1 cut(s) 472
FaeI CATG 1 cut(s) 245
FaiI YATR 6 cut(s) 22, 24, 26, 32, 243, 468
FatI CATG 1 cut(s) 241
Fnu4HI GCNGC 2 cut(s) 378, 497
FokI GGATG 1 cut(s) 457
Fsp4HI GCNGC 2 cut(s) 378, 497
GlaI GCGC 1 cut(s) 510
GluI GCNGC 2 cut(s) 378, 497
HaeIII GGCC 1 cut(s) 213
HapII CCGG 4 cut(s) 11, 210, 383, 425
HhaI GCGC 1 cut(s) 511
Hin1II CATG 1 cut(s) 245
Hin6I GCGC 1 cut(s) 509
HinP1I GCGC 1 cut(s) 509
HinfI GANTC 2 cut(s) 458, 481
HpaII CCGG 4 cut(s) 11, 210, 383, 425
Hpy166II GTNNAC 3 cut(s) 173, 184, 422
Hpy188I TCNGA 3 cut(s) 100, 248, 434
Hpy188III TCNNGA 3 cut(s) 52, 278, 350
Hpy8I GTNNAC 3 cut(s) 173, 184, 422
HpyAV CCTTC 1 cut(s) 253
HpyCH4III ACNGT 1 cut(s) 490
HpyCH4V TGCA 6 cut(s) 59, 308, 377, 392, 445, 470
HpyF10VI GCNNNNNNNGC 2 cut(s) 73, 383
Hsp92II CATG 1 cut(s) 245
HspAI GCGC 1 cut(s) 509
Kzo9I GATC 6 cut(s) 157, 223, 238, 352, 360, 429
Lsp1109I GCAGC 2 cut(s) 389, 483
LweI GCATC 1 cut(s) 479
MaeIII GTNAC 3 cut(s) 409, 450, 478
MalI GATC 6 cut(s) 159, 225, 240, 354, 362, 431
MboI GATC 6 cut(s) 157, 223, 238, 352, 360, 429
MboII GAAGA 2 cut(s) 144, 311
MhlI GDGCHC 1 cut(s) 324
MluCI AATT 4 cut(s) 15, 116, 288, 337
MlyI GAGTC 1 cut(s) 475
MnlI CCTC 5 cut(s) 66, 241, 374, 399, 409
Mph1103I ATGCAT 1 cut(s) 472
MseI TTAA 1 cut(s) 272
MspA1I CMGCKG 1 cut(s) 332
MspI CCGG 4 cut(s) 11, 210, 383, 425
MspR9I CCNGG 2 cut(s) 426, 515
MvaI CCWGG 1 cut(s) 515
MvnI CGCG 1 cut(s) 297
MwoI GCNNNNNNNGC 2 cut(s) 73, 383
NciI CCSGG 1 cut(s) 426
NdeII GATC 6 cut(s) 157, 223, 238, 352, 360, 429
NlaIII CATG 1 cut(s) 245
NmuCI GTSAC 1 cut(s) 478
NsiI ATGCAT 1 cut(s) 472
PcsI WCGNNNNNNNCGW 1 cut(s) 438
PfeI GAWTC 1 cut(s) 458
PinAI ACCGGT 1 cut(s) 10
PkrI GCNGC 2 cut(s) 379, 498
PleI GAGTC 1 cut(s) 475
PpsI GAGTC 1 cut(s) 475
PsiI TTATAA 1 cut(s) 32
Psp6I CCWGG 1 cut(s) 513
PspEI GGTNACC 2 cut(s) 409, 450
PspGI CCWGG 1 cut(s) 513
RsaI GTAC 4 cut(s) 164, 174, 185, 423
RsaNI GTAC 4 cut(s) 163, 173, 184, 422
SaqAI TTAA 1 cut(s) 272
SatI GCNGC 2 cut(s) 378, 497
Sau3AI GATC 6 cut(s) 157, 223, 238, 352, 360, 429
SchI GAGTC 1 cut(s) 475
ScrFI CCNGG 2 cut(s) 426, 515
SduI GDGCHC 1 cut(s) 324
SetI ASST 4 cut(s) 38, 64, 441, 452
SfaNI GCATC 1 cut(s) 479
SgrAI CRCCGGYG 1 cut(s) 382
SmiI ATTTAAAT 1 cut(s) 273
Sse9I AATT 4 cut(s) 15, 116, 288, 337
SsiI CCGC 3 cut(s) 295, 332, 386
StyD4I CCNGG 2 cut(s) 424, 513
SwaI ATTTAAAT 1 cut(s) 273
TaaI ACNGT 1 cut(s) 490
TaqI TCGA 3 cut(s) 233, 318, 351
TasI AATT 4 cut(s) 15, 116, 288, 337
TatI WGTACW 3 cut(s) 162, 172, 183
TfiI GAWTC 1 cut(s) 458
Tru1I TTAA 1 cut(s) 272
Tru9I TTAA 1 cut(s) 272
TscAI CASTG 1 cut(s) 375
TseFI GTSAC 1 cut(s) 478
TseI GCWGC 2 cut(s) 377, 496
Tsp45I GTSAC 1 cut(s) 478
TspDTI ATGAA 3 cut(s) 17, 272, 304
TspGWI ACGGA 1 cut(s) 152
TspRI CASTG 1 cut(s) 375
Zsp2I ATGCAT 1 cut(s) 472
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.