FvH4_7g08221

Phosphopantothenate-cysteine ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0001987
Physical Location & Seq
Reverse (-)
8177355 .. 8186683
9329 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g08221.t2

Sequence Viewer

Length: 1098 bp
ATGAACGAGGATTATAGAAGTTCATTGTGTGGGCAGGTGCAGCGTATCAAGCTAGTGACTAGTTTACATGGGATTATAGGCTCAGATAAAACCTATAAATCTGATTTCCATAAACCCGGGTTGAAAAATACGACCGTTTTCTCAGTTCCTGACAACTTCAGAGTTCACACCATCTTTCTCCCAACCGGACCGGACCGGCGGTTTAAGCGGTTTTGCTGTGATTGGCTTCCTGCACCACTCAAAGAGGCAAATTTTGGAATGGCTCTAACAAATGGGTTGGAAATTCCTAAAGAAGTGCTAGATGCAGAAATCAAATCCTTTTATGACTCAGCTCCTCCTTTAAACAATAGTGATGATGTCAGTGAAAAACTTGAGGCATTTGTTAAAAAAAATTCGCAGCCTTCTGGAAATGGAGGAGCTAGGAGGGTGGTTTGTGTAACCTCTGGTGGTACCACGGTTCCTCTGGAGCAACGATGTGTTCGCTACATTGACAACTTCAGTTCTGGTCACAGAGGAGCAGCTTCCACAGAGTACTTTTTGAAGGCAGGCTATGCTGTTATCTTTCTTTACCGGAGGGGAACTTGCCAACCATATTGCAGATCTCTTCCCAATGATCCATTACTTGAATGTCTGGAAATTGTGAACGAGTCAAACATTCAAGTACATCAGTCCCATTCACAAGCAGTGAGGAGTGCTATCACTGAGACTCATGCTGCGGTGGCAGACGGCCTCTTGCTGAAACTTCCCTTTACAACAATATTTGAGTATCTCCAGGCAGAGCACAAAATTCAATCAGGGTCTGGTCCATTGGATATGCGACTTGTTCAGGCGCCAAAGATGCTCTTAGTGCTGAGGAAAGACTGGGCTCCCATGGCCTTCTGCATATCATTTAAGCTAGAAACAGATTCAAAGATCCTGTTAGAGAAGGCTGATATGGCTTTTAGGAAGTACAAGATGCATATGGTTGTAGCCAATGAGCTCTTGAGCCGAAAAGAAGAGATCGTTGTTGTCACCAGCAATGAAAAGATTTCAGTTTGTCGAAACAAGTCTCTGGGTGTTGATGATGTTGAGAGCGCCCTTATTGAGCTTCTTGTGTAG

Protein Analysis

366

Amino Acids

40.8

Weight (kDa)

8.25

Isoelectric Point (pI)

40.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DFP PF04127 263 - 380 2.6e-09 DNA/pantothenate metabolism flavoprotein C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 25
Acc36I ACCTGC 1 cut(s) 25
Acc65I GGTACC 1 cut(s) 449
AccB1I GGYRCC 2 cut(s) 449, 829
AciI CCGC 3 cut(s) 199, 208, 716
AclWI GGATC 2 cut(s) 608, 907
AcsI RAATTY 4 cut(s) 250, 282, 391, 786
AcuI CTGAAG 2 cut(s) 142, 481
AcyI GRCGYC 1 cut(s) 830
AfaI GTAC 4 cut(s) 451, 533, 663, 950
AgsI TTSAA 6 cut(s) 124, 541, 626, 659, 791, 909
AhlI ACTAGT 1 cut(s) 59
AjnI CCWGG 1 cut(s) 771
AluBI AGCT 7 cut(s) 52, 332, 419, 521, 895, 979, 1087
AluI AGCT 7 cut(s) 52, 332, 419, 521, 895, 979, 1087
Alw21I GWGCWC 2 cut(s) 783, 981
Alw26I GTCTC 2 cut(s) 698, 1053
AlwI GGATC 2 cut(s) 608, 907
AlwNI CAGNNNCTG 2 cut(s) 149, 800
Ama87I CYCGRG 1 cut(s) 116
AoxI GGCC 2 cut(s) 727, 873
ApeKI GCWGC 4 cut(s) 40, 397, 518, 713
ApoI RAATTY 4 cut(s) 250, 282, 391, 786
Asp718I GGTACC 1 cut(s) 449
AspLEI GCGC 2 cut(s) 832, 1076
AspS9I GGNCC 3 cut(s) 188, 193, 803
AsuC2I CCSGG 2 cut(s) 117, 118
AsuHPI GGTGA 1 cut(s) 1003
AvaI CYCGRG 1 cut(s) 116
AvaII GGWCC 3 cut(s) 188, 193, 803
BaeI ACNNNNGTAYC 2 cut(s) 441, 474
BanI GGYRCC 2 cut(s) 449, 829
BanII GRGCYC 2 cut(s) 868, 981
Bbv12I GWGCWC 2 cut(s) 783, 981
BbvCI CCTCAGC 1 cut(s) 851
BbvI GCAGC 4 cut(s) 52, 409, 530, 700
BccI CCATC 1 cut(s) 179
BceAI ACGGC 1 cut(s) 742
BciT130I CCWGG 1 cut(s) 773
BcnI CCSGG 2 cut(s) 117, 118
BcoDI GTCTC 2 cut(s) 698, 1053
BcuI ACTAGT 1 cut(s) 59
BfaI CTAG 5 cut(s) 53, 60, 299, 420, 896
BfoI RGCGCY 2 cut(s) 833, 1077
BfuAI ACCTGC 1 cut(s) 25
BglII AGATCT 1 cut(s) 599
BisI GCNGC 4 cut(s) 41, 398, 519, 714
BlsI GCNGC 4 cut(s) 42, 399, 520, 715
BmcAI AGTACT 1 cut(s) 533
Bme1390I CCNGG 3 cut(s) 117, 118, 773
Bme18I GGWCC 3 cut(s) 188, 193, 803
BmeT110I CYCGRG 1 cut(s) 116
BmgT120I GGNCC 3 cut(s) 188, 193, 803
BmiI GGNNCC 4 cut(s) 451, 459, 831, 867
BmrFI CCNGG 3 cut(s) 117, 118, 773
BmrI ACTGGG 1 cut(s) 871
BmsI GCATC 3 cut(s) 292, 828, 945
BmuI ACTGGG 1 cut(s) 871
BpmI CTGGAG 2 cut(s) 485, 755
Bpu10I CCTNAGC 1 cut(s) 851
BpuEI CTTGAG 2 cut(s) 392, 1003
BpuMI CCSGG 2 cut(s) 117, 118
BsaHI GRCGYC 1 cut(s) 830
BsaJI CCNNGG 3 cut(s) 116, 453, 870
BsaWI WCCGGW 3 cut(s) 185, 190, 570
Bse118I RCCGGY 1 cut(s) 195
Bse1I ACTGG 1 cut(s) 866
Bse3DI GCAATG 1 cut(s) 1024
BseBI CCWGG 1 cut(s) 773
BseDI CCNNGG 3 cut(s) 116, 453, 870
BseMI GCAATG 1 cut(s) 1024
BseMII CTCAG 5 cut(s) 96, 156, 342, 693, 842
BseNI ACTGG 1 cut(s) 866
BseRI GAGGAG 4 cut(s) 324, 429, 528, 703
BseXI GCAGC 4 cut(s) 52, 409, 530, 700
BsgI GTGCAG 2 cut(s) 59, 216
Bsh1285I CGRYCG 1 cut(s) 135
BshFI GGCC 2 cut(s) 729, 875
BshNI GGYRCC 2 cut(s) 449, 829
BsiEI CGRYCG 1 cut(s) 135
BsiHKAI GWGCWC 2 cut(s) 783, 981
BsiHKCI CYCGRG 1 cut(s) 116
BsiSI CCGG 5 cut(s) 117, 186, 191, 196, 571
BslFI GGGAC 1 cut(s) 655
BsmAI GTCTC 2 cut(s) 698, 1053
BsmFI GGGAC 1 cut(s) 655
BsnI GGCC 2 cut(s) 729, 875
BsoBI CYCGRG 1 cut(s) 116
Bsp1286I GDGCHC 3 cut(s) 783, 868, 981
Bsp143I GATC 4 cut(s) 599, 613, 912, 999
Bsp19I CCATGG 1 cut(s) 870
BspACI CCGC 3 cut(s) 199, 208, 716
BspANI GGCC 2 cut(s) 729, 875
BspCNI CTCAG 5 cut(s) 95, 155, 341, 694, 843
BspLI GGNNCC 4 cut(s) 451, 459, 831, 867
BspMI ACCTGC 1 cut(s) 25
BspPI GGATC 2 cut(s) 608, 907
BspT107I GGYRCC 2 cut(s) 449, 829
BsrDI GCAATG 1 cut(s) 1024
BsrFI RCCGGY 1 cut(s) 195
BsrI ACTGG 1 cut(s) 866
BssAI RCCGGY 1 cut(s) 195
BssECI CCNNGG 3 cut(s) 116, 453, 870
BssMI GATC 4 cut(s) 599, 613, 912, 999
BssNI GRCGYC 1 cut(s) 830
BssT1I CCWWGG 1 cut(s) 870
Bst2UI CCWGG 1 cut(s) 773
Bst4CI ACNGT 2 cut(s) 136, 457
Bst6I CTCTTC 2 cut(s) 609, 990
BstACI GRCGYC 1 cut(s) 830
BstAPI GCANNNNNTGC 1 cut(s) 551
BstC8I GCNNGC 1 cut(s) 547
BstDEI CTNAG 6 cut(s) 82, 142, 328, 702, 844, 851
BstDSI CCRYGG 2 cut(s) 453, 870
BstH2I RGCGCY 2 cut(s) 833, 1077
BstHHI GCGC 2 cut(s) 832, 1076
BstKTI GATC 4 cut(s) 602, 616, 915, 1002
BstMAI GTCTC 2 cut(s) 698, 1053
BstMBI GATC 4 cut(s) 599, 613, 912, 999
BstMCI CGRYCG 1 cut(s) 135
BstMWI GCNNNNNNNGC 9 cut(s) 40, 49, 205, 551, 719, 838, 847, 872, 935
BstNI CCWGG 1 cut(s) 773
BstSCI CCNGG 3 cut(s) 115, 116, 771
BstV1I GCAGC 4 cut(s) 52, 409, 530, 700
BstX2I RGATCY 2 cut(s) 599, 912
BstYI RGATCY 2 cut(s) 599, 912
BsuRI GGCC 2 cut(s) 729, 875
BtgI CCRYGG 2 cut(s) 453, 870
BtsI GCAGTG 1 cut(s) 690
BtsIMutI CAGTG 3 cut(s) 367, 690, 699
BveI ACCTGC 1 cut(s) 25
Cac8I GCNNGC 1 cut(s) 547
CaiI CAGNNNCTG 2 cut(s) 149, 800
CfoI GCGC 2 cut(s) 832, 1076
Cfr10I RCCGGY 1 cut(s) 195
Cfr13I GGNCC 3 cut(s) 188, 193, 803
Cfr9I CCCGGG 1 cut(s) 116
CpoI CGGWCCG 2 cut(s) 188, 193
Csp6I GTAC 4 cut(s) 450, 532, 662, 949
CspI CGGWCCG 2 cut(s) 188, 193
CviAII CATG 3 cut(s) 68, 710, 871
CviQI GTAC 4 cut(s) 450, 532, 662, 949
DdeI CTNAG 6 cut(s) 82, 142, 328, 702, 844, 851
DinI GGCGCC 1 cut(s) 831
DpnI GATC 4 cut(s) 601, 615, 914, 1001
DpnII GATC 4 cut(s) 599, 613, 912, 999
DraI TTTAAA 1 cut(s) 342
Eam1104I CTCTTC 2 cut(s) 609, 990
EarI CTCTTC 2 cut(s) 609, 990
Ecl136II GAGCTC 1 cut(s) 979
Eco130I CCWWGG 1 cut(s) 870
Eco24I GRGCYC 2 cut(s) 868, 981
Eco47I GGWCC 3 cut(s) 188, 193, 803
Eco53kI GAGCTC 1 cut(s) 979
Eco57I CTGAAG 2 cut(s) 142, 481
Eco88I CYCGRG 1 cut(s) 116
EcoICRI GAGCTC 1 cut(s) 979
EcoRII CCWGG 1 cut(s) 771
EcoT14I CCWWGG 1 cut(s) 870
EcoT22I ATGCAT 1 cut(s) 960
EcoT38I GRGCYC 2 cut(s) 868, 981
EgeI GGCGCC 1 cut(s) 831
EheI GGCGCC 1 cut(s) 831
ErhI CCWWGG 1 cut(s) 870
FaeI CATG 3 cut(s) 71, 713, 874
FalI AAGNNNNNCTT 2 cut(s) 827, 859
FaqI GGGAC 1 cut(s) 655
FatI CATG 3 cut(s) 67, 709, 870
FauNDI CATATG 1 cut(s) 960
Fnu4HI GCNGC 4 cut(s) 41, 398, 519, 714
FriOI GRGCYC 2 cut(s) 868, 981
Fsp4HI GCNGC 4 cut(s) 41, 398, 519, 714
FspBI CTAG 5 cut(s) 53, 60, 299, 420, 896
GlaI GCGC 2 cut(s) 831, 1075
GluI GCNGC 4 cut(s) 41, 398, 519, 714
GsuI CTGGAG 2 cut(s) 485, 755
HaeII RGCGCY 2 cut(s) 833, 1077
HaeIII GGCC 2 cut(s) 729, 875
HapII CCGG 5 cut(s) 117, 186, 191, 196, 571
HhaI GCGC 2 cut(s) 832, 1076
Hin1I GRCGYC 1 cut(s) 830
Hin1II CATG 3 cut(s) 71, 713, 874
Hin6I GCGC 2 cut(s) 830, 1074
HinP1I GCGC 2 cut(s) 830, 1074
HinfI GANTC 4 cut(s) 326, 647, 706, 905
HpaII CCGG 5 cut(s) 117, 186, 191, 196, 571
HphI GGTGA 1 cut(s) 1003
Hpy166II GTNNAC 3 cut(s) 65, 166, 643
Hpy188I TCNGA 3 cut(s) 85, 103, 161
Hpy188III TCNNGA 5 cut(s) 149, 405, 464, 632, 982
Hpy8I GTNNAC 3 cut(s) 65, 166, 643
HpyAV CCTTC 4 cut(s) 411, 535, 886, 919
HpyCH4III ACNGT 2 cut(s) 136, 457
HpyCH4V TGCA 6 cut(s) 40, 233, 305, 597, 882, 958
HpyF10VI GCNNNNNNNGC 9 cut(s) 40, 49, 205, 551, 719, 838, 847, 872, 935
HpyF3I CTNAG 6 cut(s) 82, 142, 328, 702, 844, 851
Hsp92I GRCGYC 1 cut(s) 830
Hsp92II CATG 3 cut(s) 71, 713, 874
HspAI GCGC 2 cut(s) 830, 1074
KasI GGCGCC 1 cut(s) 829
KpnI GGTACC 1 cut(s) 453
Kzo9I GATC 4 cut(s) 599, 613, 912, 999
LmnI GCTCC 5 cut(s) 337, 416, 466, 515, 871
Lsp1109I GCAGC 4 cut(s) 52, 409, 530, 700
LweI GCATC 3 cut(s) 292, 828, 945
MaeI CTAG 5 cut(s) 53, 60, 299, 420, 896
MaeIII GTNAC 4 cut(s) 55, 436, 506, 1009
MalI GATC 4 cut(s) 601, 615, 914, 1001
MboI GATC 4 cut(s) 599, 613, 912, 999
MboII GAAGA 2 cut(s) 596, 1007
MflI RGATCY 2 cut(s) 599, 912
MhlI GDGCHC 3 cut(s) 783, 868, 981
MluCI AATT 5 cut(s) 250, 282, 391, 636, 786
Mly113I GGCGCC 1 cut(s) 830
MlyI GAGTC 3 cut(s) 320, 656, 700
MmeI TCCRAC 1 cut(s) 258
Mph1103I ATGCAT 1 cut(s) 960
MseI TTAA 4 cut(s) 204, 341, 384, 891
MspI CCGG 5 cut(s) 117, 186, 191, 196, 571
MspR9I CCNGG 3 cut(s) 117, 118, 773
MvaI CCWGG 1 cut(s) 773
MwoI GCNNNNNNNGC 9 cut(s) 40, 49, 205, 551, 719, 838, 847, 872, 935
NarI GGCGCC 1 cut(s) 830
NciI CCSGG 2 cut(s) 117, 118
NcoI CCATGG 1 cut(s) 870
NdeI CATATG 1 cut(s) 960
NdeII GATC 4 cut(s) 599, 613, 912, 999
NlaIII CATG 3 cut(s) 71, 713, 874
NlaIV GGNNCC 4 cut(s) 451, 459, 831, 867
NmuCI GTSAC 3 cut(s) 55, 506, 1009
NsiI ATGCAT 1 cut(s) 960
PaqCI CACCTGC 1 cut(s) 25
PfeI GAWTC 1 cut(s) 905
PkrI GCNGC 4 cut(s) 42, 399, 520, 715
PleI GAGTC 3 cut(s) 320, 655, 700
PluTI GGCGCC 1 cut(s) 833
PpsI GAGTC 3 cut(s) 320, 655, 700
Psp124BI GAGCTC 1 cut(s) 981
Psp6I CCWGG 1 cut(s) 771
PspGI CCWGG 1 cut(s) 771
PspN4I GGNNCC 4 cut(s) 451, 459, 831, 867
PspPI GGNCC 3 cut(s) 188, 193, 803
PstNI CAGNNNCTG 2 cut(s) 149, 800
PsuI RGATCY 2 cut(s) 599, 912
RsaI GTAC 4 cut(s) 451, 533, 663, 950
RsaNI GTAC 4 cut(s) 450, 532, 662, 949
Rsr2I CGGWCCG 2 cut(s) 188, 193
RsrII CGGWCCG 2 cut(s) 188, 193
SacI GAGCTC 1 cut(s) 981
SaqAI TTAA 4 cut(s) 204, 341, 384, 891
SatI GCNGC 4 cut(s) 41, 398, 519, 714
Sau3AI GATC 4 cut(s) 599, 613, 912, 999
Sau96I GGNCC 3 cut(s) 188, 193, 803
ScaI AGTACT 1 cut(s) 533
SchI GAGTC 3 cut(s) 320, 656, 700
ScrFI CCNGG 3 cut(s) 117, 118, 773
SduI GDGCHC 3 cut(s) 783, 868, 981
SfaNI GCATC 3 cut(s) 292, 828, 945
SfoI GGCGCC 1 cut(s) 831
SinI GGWCC 3 cut(s) 188, 193, 803
SmaI CCCGGG 1 cut(s) 118
SmlI CTYRAG 2 cut(s) 371, 982
SmoI CTYRAG 2 cut(s) 371, 982
SpeI ACTAGT 1 cut(s) 59
Sse9I AATT 5 cut(s) 250, 282, 391, 636, 786
SsiI CCGC 3 cut(s) 199, 208, 716
SspDI GGCGCC 1 cut(s) 829
SspI AATATT 1 cut(s) 759
SspMI CTAG 5 cut(s) 53, 60, 299, 420, 896
SstI GAGCTC 1 cut(s) 981
StyD4I CCNGG 3 cut(s) 115, 116, 771
StyI CCWWGG 1 cut(s) 870
TaaI ACNGT 2 cut(s) 136, 457
TaqI TCGA 1 cut(s) 1039
TasI AATT 5 cut(s) 250, 282, 391, 636, 786
TatI WGTACW 3 cut(s) 531, 661, 948
TfiI GAWTC 1 cut(s) 905
Tru1I TTAA 4 cut(s) 204, 341, 384, 891
Tru9I TTAA 4 cut(s) 204, 341, 384, 891
TscAI CASTG 3 cut(s) 367, 690, 706
TseFI GTSAC 3 cut(s) 55, 506, 1009
TseI GCWGC 4 cut(s) 40, 397, 518, 713
Tsp45I GTSAC 3 cut(s) 55, 506, 1009
TspDTI ATGAA 3 cut(s) 12, 17, 1035
TspMI CCCGGG 1 cut(s) 116
TspRI CASTG 3 cut(s) 367, 690, 706
VpaK11BI GGWCC 3 cut(s) 188, 193, 803
XapI RAATTY 4 cut(s) 250, 282, 391, 786
XcmI CCANNNNNNNNNTGG 1 cut(s) 460
XmaI CCCGGG 1 cut(s) 116
XspI CTAG 5 cut(s) 53, 60, 299, 420, 896
ZrmI AGTACT 1 cut(s) 533
Zsp2I ATGCAT 1 cut(s) 960
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.