Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr1D

Orthogroup ID

OG0001987

Physical Location & Seq

Genomic Coordinates Reverse (-)

35976531 .. 35982868

Gene Structure 6338 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh1DG175600.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1167 bp

ATGGGGCATGGGCACATCTGCCCCAATGGCAGGTCCAAGATAACAAAATATAGACTTCTCTACATGGGATTATACGCTCTAATAACCTATAAATCTGATTTTCTAAACCCGTTGGGTTGGGACCGTGTTCACCGGTGCTGTGGTTGGCATCCTCTGCAACTCAAAAAGGCAAATTACAAAATGGCTCTAACAAATGGGTTGGAAATTCCTAAAGAAGTGCTGGATGCAGAAATCAAATCCTTTTTTGACTCAGCTCCTCCTTTAAAGAACAGAGATGATGTCAGTGAAAAACTTGAGGCATTTGTTAAAAAGAATTCGCAGCCTTCTGGAAATGGAGGAGCTAGGAGGGTGGTTTGTGTAACCTCTGGTGGTACCACGGTTCCTCTGGAGCAACGATGTGTTCGCTACATTGACAACTTCAGCTCAGGTTACAGAGGAGCAGCTTCCACAGAGTACTTTTTGAAGGCAGGCTGTGCTGTTATCTTTCTTTATCGGAGGGGAACTTGCCAACCATATTGCAGATCTCTTCCTGATGATCTGATGTCAGATTTTGGAAGGATGGTGGAGTGTTATGATCCGAAAGTACACCAATCCCATTCACAAGCAGTGAGGAGTGCTATCACTAAGAGTCATGCTGCAGTAGCAGACGGCCTCTTGCTGAAACTTCCCTTTACAACAATATTTGAGTATCTCCAGATGTTGCAGATGGTTGCGTTTACAATGCGAAGTATTGGACCACATGCCATGCTATATCTTGCAGCAGCAGTATCTGACTTTTACGTCCCTTGGAAGAGCATGGCAGAGCACAAAATTCAGTCAGGGTCTGGTCCATTGGATATGCGACTTGTTCAGGTGCCAAAGATGCTCTTAGTGCTGAGGAAAGACTGGGCTCCCCTGGCCTTCTGCATATCATTCAAGCTAGAAACAGATTCAAAGATCCTGTTAGAGAAGGCTGATATGGCTCGTAGGAAGTACAAGATGCATATGGTTGTAGCCAATGAGCTTTTGAGCCGAAAAGAAGAGGTTGTAGTTGTCACCAGCAATGAAAAGATTCTAGTTCGCCGAAACAAGTCTCTGGGTGTTGATGATGTGGAGAGCGCCCTTATTGAGCTTCTTGTGGAGAGGCATTCAGCTTATGTTGATCAGATCAAGACTGATTTTATATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004632 GO:0006139 GO:0006163 GO:0006164 GO:0006725 GO:0006732 GO:0006753 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009108 GO:0009117 GO:0009150 GO:0009152 GO:0009165 GO:0009259 GO:0009260 GO:0009987 GO:0015936 GO:0015937 GO:0016874 GO:0016879 GO:0016881 GO:0018130 GO:0019438 GO:0019637 GO:0019693 GO:0033865 GO:0033866 GO:0033875 GO:0034030 GO:0034032 GO:0034033 GO:0034641 GO:0034654 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044281 GO:0046390 GO:0046483 GO:0051186 GO:0051188 GO:0055086 GO:0071704 GO:0072521 GO:0072522 GO:0090407 GO:1901135 GO:1901137 GO:1901293 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00770 ko01100 map00770 map01100

Pfam Domains

Protein Families

DFP

Protein Analysis

388

Amino Acids

43.85

Weight (kDa)

9.18

Isoelectric Point (pI)

43.0

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DFP	PF04127	235 - 357	2.9e-10	DNA/pantothenate metabolism flavoprotein C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001987)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G12350 AT1G12350 AT5G02080 AT5G02080 AT5G02080 AT5G02080 AT5G02080 AT5G02080 AT5G02080
fragaria_vesca	FvH4_5g38402 FvH4_5g38402 FvH4_5g38420 FvH4_5g38420 FvH4_7g08221 FvH4_7g08221
malus_domestica	MD08G1174000.v1.1 MD15G1360400.v1.1
prunus_persica	Prupe.1G507900_v2.0.a1 Prupe.1G507900_v2.0.a1 Prupe.1G507900_v2.0.a1 Prupe.1G507900_v2.0.a1
pyrus_communis	pycom08g14920 pycom15g32230
rosa_chinensis	RchiOBHm_Chr1g0340891 RchiOBHm_Chr1g0340911 RchiOBHm_Chr7g0240421
rosa_laevigata	RLG00000000678 RLG00000029049
rosa_multiflora	Rmu_sc0001211.1_g000112 Rmu_sc0002676.1_g000001
rosa_roxburghii	Rroxscaffold_3G00220100
rosa_rugosa	Rorug01G0160900.1 Rorug07G0335900
rosa_samantha	Rh1AG175900 Rh1BG143900 Rh1CG163800 Rh1DG175600 Rh7AG490200 Rh7BG463100 Rh7CG506600 Rh7DG474200
rosa_wichuraiana	Rw0G004950 Rw1G014660 Rw7G041000 Rw7G041050

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	779
Acc36I	ACCTGC	1 cut(s)	21
Acc65I	GGTACC	1 cut(s)	371
AccB1I	GGYRCC	2 cut(s)	371, 853
AclWI	GGATC	2 cut(s)	569, 931
AcsI	RAATTY	3 cut(s)	204, 313, 810
AcuI	CTGAAG	1 cut(s)	403
AfaI	GTAC	4 cut(s)	373, 455, 585, 974
AfiI	CCNNNNNNNGG	1 cut(s)	30
AgeI	ACCGGT	1 cut(s)	132
AgsI	TTSAA	3 cut(s)	463, 916, 933
AjnI	CCWGG	1 cut(s)	894
AluBI	AGCT	8 cut(s)	254, 341, 423, 443, 919, 1003, 1111, 1133
AluI	AGCT	8 cut(s)	254, 341, 423, 443, 919, 1003, 1111, 1133
Alw21I	GWGCWC	1 cut(s)	807
Alw26I	GTCTC	1 cut(s)	1077
AlwI	GGATC	2 cut(s)	569, 931
AlwNI	CAGNNNCTG	2 cut(s)	770, 824
AoxI	GGCC	2 cut(s)	649, 897
ApeKI	GCWGC	5 cut(s)	319, 440, 635, 758, 761
ApoI	RAATTY	3 cut(s)	204, 313, 810
AsiGI	ACCGGT	1 cut(s)	132
Asp700I	GAANNNNTTC	1 cut(s)	1050
Asp718I	GGTACC	1 cut(s)	371
AspLEI	GCGC	1 cut(s)	1100
AspS9I	GGNCC	4 cut(s)	33, 121, 734, 827
AsuHPI	GGTGA	2 cut(s)	122, 1027
AvaII	GGWCC	4 cut(s)	33, 121, 734, 827
BaeGI	GKGCMC	1 cut(s)	15
BaeI	ACNNNNGTAYC	2 cut(s)	363, 396
BanI	GGYRCC	2 cut(s)	371, 853
BanII	GRGCYC	1 cut(s)	892
Bbv12I	GWGCWC	1 cut(s)	807
BbvCI	CCTCAGC	1 cut(s)	875
BbvI	GCAGC	5 cut(s)	331, 452, 622, 770, 773
BccI	CCATC	2 cut(s)	553, 700
BceAI	ACGGC	1 cut(s)	664
BciT130I	CCWGG	1 cut(s)	896
BclI	TGATCA	1 cut(s)	1141
BcoDI	GTCTC	1 cut(s)	1077
BfaI	CTAG	3 cut(s)	342, 920, 1055
BfmI	CTRYAG	1 cut(s)	636
BfoI	RGCGCY	1 cut(s)	1101
BfuAI	ACCTGC	1 cut(s)	21
BglI	GCCNNNNNGGC	1 cut(s)	27
BglII	AGATCT	1 cut(s)	521
BisI	GCNGC	5 cut(s)	320, 441, 636, 759, 762
BlsI	GCNGC	5 cut(s)	321, 442, 637, 760, 763
BmcAI	AGTACT	1 cut(s)	455
Bme1390I	CCNGG	1 cut(s)	896
Bme18I	GGWCC	4 cut(s)	33, 121, 734, 827
BmgT120I	GGNCC	4 cut(s)	33, 121, 734, 827
BmiI	GGNNCC	5 cut(s)	122, 373, 381, 855, 891
BmrFI	CCNGG	1 cut(s)	896
BmrI	ACTGGG	1 cut(s)	895
BmsI	GCATC	4 cut(s)	157, 214, 852, 969
BmuI	ACTGGG	1 cut(s)	895
BpmI	CTGGAG	2 cut(s)	407, 677
Bpu10I	CCTNAGC	2 cut(s)	424, 875
BpuEI	CTTGAG	1 cut(s)	314
BsaJI	CCNNGG	3 cut(s)	375, 785, 894
BsaWI	WCCGGW	1 cut(s)	132
Bsc4I	CCNNNNNNNGG	1 cut(s)	30
Bse118I	RCCGGY	1 cut(s)	132
Bse1I	ACTGG	1 cut(s)	890
Bse3DI	GCAATG	1 cut(s)	1048
BseBI	CCWGG	1 cut(s)	896
BseDI	CCNNGG	3 cut(s)	375, 785, 894
BseGI	GGATG	3 cut(s)	148, 229, 564
BseLI	CCNNNNNNNGG	1 cut(s)	30
BseMI	GCAATG	1 cut(s)	1048
BseMII	CTCAG	3 cut(s)	264, 438, 866
BseNI	ACTGG	1 cut(s)	890
BseRI	GAGGAG	4 cut(s)	246, 351, 450, 625
BseSI	GKGCMC	1 cut(s)	15
BseXI	GCAGC	5 cut(s)	331, 452, 622, 770, 773
BshFI	GGCC	2 cut(s)	651, 899
BshNI	GGYRCC	2 cut(s)	371, 853
BshTI	ACCGGT	1 cut(s)	132
BsiHKAI	GWGCWC	1 cut(s)	807
BsiSI	CCGG	1 cut(s)	133
BslFI	GGGAC	2 cut(s)	134, 767
BslI	CCNNNNNNNGG	1 cut(s)	30
BsmAI	GTCTC	1 cut(s)	1077
BsmFI	GGGAC	2 cut(s)	134, 767
BsmI	GAATGC	1 cut(s)	1126
BsnI	GGCC	2 cut(s)	651, 899
Bsp1286I	GDGCHC	3 cut(s)	15, 807, 892
Bsp143I	GATC	6 cut(s)	521, 535, 574, 936, 1141, 1146
BspANI	GGCC	2 cut(s)	651, 899
BspCNI	CTCAG	3 cut(s)	263, 437, 867
BspLI	GGNNCC	5 cut(s)	122, 373, 381, 855, 891
BspMAI	CTGCAG	1 cut(s)	640
BspMI	ACCTGC	1 cut(s)	21
BspPI	GGATC	2 cut(s)	569, 931
BspQI	GCTCTTC	1 cut(s)	785
BspT107I	GGYRCC	2 cut(s)	371, 853
BsrDI	GCAATG	1 cut(s)	1048
BsrFI	RCCGGY	1 cut(s)	132
BsrI	ACTGG	1 cut(s)	890
BssAI	RCCGGY	1 cut(s)	132
BssECI	CCNNGG	3 cut(s)	375, 785, 894
BssMI	GATC	6 cut(s)	521, 535, 574, 936, 1141, 1146
BssT1I	CCWWGG	1 cut(s)	785
Bst2UI	CCWGG	1 cut(s)	896
Bst4CI	ACNGT	2 cut(s)	125, 379
Bst6I	CTCTTC	3 cut(s)	531, 785, 1014
BstAPI	GCANNNNNTGC	2 cut(s)	154, 473
BstC8I	GCNNGC	1 cut(s)	469
BstDEI	CTNAG	5 cut(s)	250, 424, 624, 868, 875
BstDSI	CCRYGG	1 cut(s)	375
BstF5I	GGATG	3 cut(s)	148, 229, 564
BstH2I	RGCGCY	1 cut(s)	1101
BstHHI	GCGC	1 cut(s)	1100
BstKTI	GATC	6 cut(s)	524, 538, 577, 939, 1144, 1149
BstMAI	GTCTC	1 cut(s)	1077
BstMBI	GATC	6 cut(s)	521, 535, 574, 936, 1141, 1146
BstMWI	GCNNNNNNNGC	8 cut(s)	27, 154, 473, 641, 862, 871, 896, 959
BstNI	CCWGG	1 cut(s)	896
BstNSI	RCATGY	1 cut(s)	743
BstSCI	CCNGG	1 cut(s)	894
BstSFI	CTRYAG	1 cut(s)	636
BstSLI	GKGCMC	1 cut(s)	15
BstV1I	GCAGC	5 cut(s)	331, 452, 622, 770, 773
BstX2I	RGATCY	2 cut(s)	521, 936
BstYI	RGATCY	2 cut(s)	521, 936
BsuRI	GGCC	2 cut(s)	651, 899
BtgI	CCRYGG	1 cut(s)	375
BtsCI	GGATG	3 cut(s)	148, 229, 564
BtsI	GCAGTG	1 cut(s)	612
BtsIMutI	CAGTG	2 cut(s)	289, 612
BveI	ACCTGC	1 cut(s)	21
Cac8I	GCNNGC	1 cut(s)	469
CaiI	CAGNNNCTG	2 cut(s)	770, 824
CfoI	GCGC	1 cut(s)	1100
Cfr10I	RCCGGY	1 cut(s)	132
Cfr13I	GGNCC	4 cut(s)	33, 121, 734, 827
Csp6I	GTAC	4 cut(s)	372, 454, 584, 973
CspAI	ACCGGT	1 cut(s)	132
CviAII	CATG	6 cut(s)	8, 64, 632, 740, 745, 796
CviQI	GTAC	4 cut(s)	372, 454, 584, 973
DdeI	CTNAG	5 cut(s)	250, 424, 624, 868, 875
DpnI	GATC	6 cut(s)	523, 537, 576, 938, 1143, 1148
DpnII	GATC	6 cut(s)	521, 535, 574, 936, 1141, 1146
DraI	TTTAAA	1 cut(s)	264
DrdI	GACNNNNNNGTC	1 cut(s)	779
DseDI	GACNNNNNNGTC	1 cut(s)	779
Eam1104I	CTCTTC	3 cut(s)	531, 785, 1014
EarI	CTCTTC	3 cut(s)	531, 785, 1014
Eco130I	CCWWGG	1 cut(s)	785
Eco24I	GRGCYC	1 cut(s)	892
Eco47I	GGWCC	4 cut(s)	33, 121, 734, 827
Eco57I	CTGAAG	1 cut(s)	403
EcoRI	GAATTC	1 cut(s)	313
EcoRII	CCWGG	1 cut(s)	894
EcoT14I	CCWWGG	1 cut(s)	785
EcoT22I	ATGCAT	1 cut(s)	984
EcoT38I	GRGCYC	1 cut(s)	892
ErhI	CCWWGG	1 cut(s)	785
FaeI	CATG	6 cut(s)	11, 67, 635, 743, 748, 799
FalI	AAGNNNNNCTT	2 cut(s)	851, 883
FaqI	GGGAC	2 cut(s)	134, 767
FatI	CATG	6 cut(s)	7, 63, 631, 739, 744, 795
FauNDI	CATATG	1 cut(s)	984
FbaI	TGATCA	1 cut(s)	1141
Fnu4HI	GCNGC	5 cut(s)	320, 441, 636, 759, 762
FokI	GGATG	3 cut(s)	135, 236, 571
FriOI	GRGCYC	1 cut(s)	892
Fsp4HI	GCNGC	5 cut(s)	320, 441, 636, 759, 762
FspBI	CTAG	3 cut(s)	342, 920, 1055
GlaI	GCGC	1 cut(s)	1099
GluI	GCNGC	5 cut(s)	320, 441, 636, 759, 762
GsuI	CTGGAG	2 cut(s)	407, 677
HaeII	RGCGCY	1 cut(s)	1101
HaeIII	GGCC	2 cut(s)	651, 899
HapII	CCGG	1 cut(s)	133
HhaI	GCGC	1 cut(s)	1100
Hin1II	CATG	6 cut(s)	11, 67, 635, 743, 748, 799
Hin6I	GCGC	1 cut(s)	1098
HinP1I	GCGC	1 cut(s)	1098
HinfI	GANTC	4 cut(s)	248, 628, 929, 1051
HpaII	CCGG	1 cut(s)	133
HphI	GGTGA	2 cut(s)	122, 1027
Hpy166II	GTNNAC	3 cut(s)	130, 586, 717
Hpy188I	TCNGA	7 cut(s)	97, 495, 540, 547, 579, 772, 1146
Hpy188III	TCNNGA	5 cut(s)	327, 386, 530, 694, 1150
Hpy8I	GTNNAC	3 cut(s)	130, 586, 717
HpyAV	CCTTC	5 cut(s)	333, 457, 549, 910, 943
HpyCH4III	ACNGT	2 cut(s)	125, 379
HpyCH4IV	ACGT	1 cut(s)	780
HpyCH4V	TGCA	8 cut(s)	157, 227, 519, 638, 703, 758, 906, 982
HpyF10VI	GCNNNNNNNGC	8 cut(s)	27, 154, 473, 641, 862, 871, 896, 959
HpyF3I	CTNAG	5 cut(s)	250, 424, 624, 868, 875
HpySE526I	ACGT	1 cut(s)	780
Hsp92II	CATG	6 cut(s)	11, 67, 635, 743, 748, 799
HspAI	GCGC	1 cut(s)	1098
KpnI	GGTACC	1 cut(s)	375
Ksp22I	TGATCA	1 cut(s)	1141
Kzo9I	GATC	6 cut(s)	521, 535, 574, 936, 1141, 1146
LguI	GCTCTTC	1 cut(s)	785
LmnI	GCTCC	5 cut(s)	259, 338, 388, 437, 895
Lsp1109I	GCAGC	5 cut(s)	331, 452, 622, 770, 773
LweI	GCATC	4 cut(s)	157, 214, 852, 969
MaeI	CTAG	3 cut(s)	342, 920, 1055
MaeII	ACGT	1 cut(s)	780
MaeIII	GTNAC	3 cut(s)	358, 428, 1033
MalI	GATC	6 cut(s)	523, 537, 576, 938, 1143, 1148
MboI	GATC	6 cut(s)	521, 535, 574, 936, 1141, 1146
MboII	GAAGA	3 cut(s)	518, 802, 1031
MflI	RGATCY	2 cut(s)	521, 936
MhlI	GDGCHC	3 cut(s)	15, 807, 892
MluCI	AATT	4 cut(s)	172, 204, 313, 810
MlyI	GAGTC	2 cut(s)	242, 637
MmeI	TCCRAC	1 cut(s)	180
Mph1103I	ATGCAT	1 cut(s)	984
MroXI	GAANNNNTTC	1 cut(s)	1050
MseI	TTAA	2 cut(s)	263, 306
MspI	CCGG	1 cut(s)	133
MspR9I	CCNGG	1 cut(s)	896
Mva1269I	GAATGC	1 cut(s)	1126
MvaI	CCWGG	1 cut(s)	896
MwoI	GCNNNNNNNGC	8 cut(s)	27, 154, 473, 641, 862, 871, 896, 959
NdeI	CATATG	1 cut(s)	984
NdeII	GATC	6 cut(s)	521, 535, 574, 936, 1141, 1146
NlaIII	CATG	6 cut(s)	11, 67, 635, 743, 748, 799
NlaIV	GGNNCC	5 cut(s)	122, 373, 381, 855, 891
NmuCI	GTSAC	1 cut(s)	1033
NsiI	ATGCAT	1 cut(s)	984
NspI	RCATGY	1 cut(s)	743
PciSI	GCTCTTC	1 cut(s)	785
PctI	GAATGC	1 cut(s)	1126
PdmI	GAANNNNTTC	1 cut(s)	1050
PfeI	GAWTC	2 cut(s)	929, 1051
PinAI	ACCGGT	1 cut(s)	132
PkrI	GCNGC	5 cut(s)	321, 442, 637, 760, 763
PleI	GAGTC	2 cut(s)	242, 636
PpsI	GAGTC	2 cut(s)	242, 636
Psp6I	CCWGG	1 cut(s)	894
PspGI	CCWGG	1 cut(s)	894
PspN4I	GGNNCC	5 cut(s)	122, 373, 381, 855, 891
PspPI	GGNCC	4 cut(s)	33, 121, 734, 827
PstI	CTGCAG	1 cut(s)	640
PstNI	CAGNNNCTG	2 cut(s)	770, 824
PsuI	RGATCY	2 cut(s)	521, 936
RsaI	GTAC	4 cut(s)	373, 455, 585, 974
RsaNI	GTAC	4 cut(s)	372, 454, 584, 973
SapI	GCTCTTC	1 cut(s)	785
SaqAI	TTAA	2 cut(s)	263, 306
SatI	GCNGC	5 cut(s)	320, 441, 636, 759, 762
Sau3AI	GATC	6 cut(s)	521, 535, 574, 936, 1141, 1146
Sau96I	GGNCC	4 cut(s)	33, 121, 734, 827
ScaI	AGTACT	1 cut(s)	455
SchI	GAGTC	2 cut(s)	242, 637
ScrFI	CCNGG	1 cut(s)	896
SduI	GDGCHC	3 cut(s)	15, 807, 892
SfaNI	GCATC	4 cut(s)	157, 214, 852, 969
SfcI	CTRYAG	1 cut(s)	636
SgrAI	CRCCGGYG	1 cut(s)	132
SinI	GGWCC	4 cut(s)	33, 121, 734, 827
SmlI	CTYRAG	1 cut(s)	293
SmoI	CTYRAG	1 cut(s)	293
Sse9I	AATT	4 cut(s)	172, 204, 313, 810
SspI	AATATT	1 cut(s)	681
SspMI	CTAG	3 cut(s)	342, 920, 1055
StyD4I	CCNGG	1 cut(s)	894
StyI	CCWWGG	1 cut(s)	785
TaaI	ACNGT	2 cut(s)	125, 379
TaiI	ACGT	1 cut(s)	783
TasI	AATT	4 cut(s)	172, 204, 313, 810
TatI	WGTACW	3 cut(s)	453, 583, 972
TfiI	GAWTC	2 cut(s)	929, 1051
Tru1I	TTAA	2 cut(s)	263, 306
Tru9I	TTAA	2 cut(s)	263, 306
TscAI	CASTG	2 cut(s)	289, 612
TseFI	GTSAC	1 cut(s)	1033
TseI	GCWGC	5 cut(s)	319, 440, 635, 758, 761
Tsp45I	GTSAC	1 cut(s)	1033
TspDTI	ATGAA	1 cut(s)	1059
TspRI	CASTG	2 cut(s)	289, 612
VpaK11BI	GGWCC	4 cut(s)	33, 121, 734, 827
XapI	RAATTY	3 cut(s)	204, 313, 810
XceI	RCATGY	1 cut(s)	743
XcmI	CCANNNNNNNNNTGG	1 cut(s)	382
XmnI	GAANNNNTTC	1 cut(s)	1050
XspI	CTAG	3 cut(s)	342, 920, 1055
ZrmI	AGTACT	1 cut(s)	455
Zsp2I	ATGCAT	1 cut(s)	984

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.