FvH4_7g08330

tRNA (guanine-N(7)-)-methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0006250
Physical Location & Seq
Reverse (-)
8242259 .. 8248485
6227 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g08330.t1

Sequence Viewer

Length: 933 bp
ATGGGTTCCTGCGTCTGCTTACGTCATCACTTCTGCTACAGTGGTGGTGTGAGTACTGGTGCATTTCAAAGAAGGGGACTTTCTACTCTGTTTGGAAAGGGGGTTGTTACGACGCCGTTTTATGAGTATCACAGAAACAGAAGAAGAAGAGTGGAGGCGTCTGTTGCTACTGGGTTTAAGGAGCAAGAGCTGAGGAGCCCGGACCTCGTGGCCTTGGAATATGCTGATCTTAATCTCAGCCACAATGTTACCCAGGAACTGGGTCATGTGAGAATTAGGCAACATGTGAACCCTCTTAGCTCTTCTTTAGCTGTACCTGTGGAAGTACCTGATTGGAATCAAGTATTCAAGGACCCAACATTGCCTCTCATGGTGGACATTGGAAGTGGTAGTGGTAGATTTCTCATGTGGCTGGCGAAAAGAAACTTGGTTTTGAGGAATCATTTGGGACTGGAAATACGGCAGAAACTTGTCAAACGTGCTGATGTTTGGGTTAAAGAGCTCAGTCTTAGTAATATACATTTTATGTATGCGAATGCCACAACTTCTTTTAATCAACTAGTCTCTTCCTACCCTGGACCACTGATGCTTGTTTCAATCCTGTGCCCAGATCCGTATTTTAAGAGAAAGCATCACAAGAGACGGGTTGTACAGAAGCCTTTAGTAGATTCCATTGTGCATAACTTGGCTCCCAGCGCACAGGTGTTTATACAGTCAGATGTACATGAGGTGGCACTTGAGATGAGAAACCAATTTGATTCTGTGCATGTTCTGAAGCACATAGATGAAATTGACTCCAGCGTGTTGAGTGACAGTGAGGGATGGTTGTTGAGCAATCCAATGGGAATAAGGACGGAAAGAGAAATTCATGCTGAATTTGAAGGGGCAAAAATTTACAGGAGAGTGTACCAGAAGCAAGTCAACTGTTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_4

Protein Analysis

311

Amino Acids

35.4

Weight (kDa)

9.28

Isoelectric Point (pI)

52.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_4 PF02390 122 - 299 1e-29 Putative methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 259
AclWI GGATC 1 cut(s) 605
AcsI RAATTY 3 cut(s) 864, 875, 891
AcuI CTGAAG 1 cut(s) 794
AcyI GRCGYC 2 cut(s) 113, 158
AfaI GTAC 6 cut(s) 55, 315, 327, 651, 723, 908
AfiI CCNNNNNNNGG 1 cut(s) 259
AflIII ACRYGT 1 cut(s) 283
AgsI TTSAA 4 cut(s) 68, 349, 597, 881
AhlI ACTAGT 1 cut(s) 559
AjnI CCWGG 2 cut(s) 252, 574
AjuI GAANNNNNNNTTGG 2 cut(s) 410, 442
AluBI AGCT 4 cut(s) 190, 300, 311, 502
AluI AGCT 4 cut(s) 190, 300, 311, 502
Alw21I GWGCWC 1 cut(s) 504
Alw26I GTCTC 2 cut(s) 568, 634
AlwI GGATC 1 cut(s) 605
AlwNI CAGNNNCTG 1 cut(s) 259
AoxI GGCC 1 cut(s) 210
ApoI RAATTY 3 cut(s) 864, 875, 891
AspLEI GCGC 1 cut(s) 698
AspS9I GGNCC 3 cut(s) 202, 352, 578
AsuC2I CCSGG 1 cut(s) 200
AvaII GGWCC 3 cut(s) 202, 352, 578
BaeGI GKGCMC 1 cut(s) 608
BanII GRGCYC 2 cut(s) 200, 504
BauI CACGAG 1 cut(s) 206
Bbv12I GWGCWC 1 cut(s) 504
BbvCI CCTCAGC 1 cut(s) 191
BccI CCATC 1 cut(s) 816
BceAI ACGGC 2 cut(s) 100, 476
BciT130I CCWGG 2 cut(s) 254, 576
BcnI CCSGG 1 cut(s) 200
BcoDI GTCTC 2 cut(s) 568, 634
BcuI ACTAGT 1 cut(s) 559
BfaI CTAG 1 cut(s) 560
BfmI CTRYAG 1 cut(s) 37
BmcAI AGTACT 1 cut(s) 55
Bme1390I CCNGG 3 cut(s) 200, 254, 576
Bme18I GGWCC 3 cut(s) 202, 352, 578
BmgT120I GGNCC 3 cut(s) 202, 352, 578
BmiI GGNNCC 4 cut(s) 7, 197, 354, 690
BmrFI CCNGG 3 cut(s) 200, 254, 576
BmrI ACTGGG 2 cut(s) 180, 269
BmsI GCATC 2 cut(s) 576, 640
BmuI ACTGGG 2 cut(s) 180, 269
BpmI CTGGAG 1 cut(s) 781
Bpu10I CCTNAGC 1 cut(s) 191
BpuEI CTTGAG 1 cut(s) 758
BpuMI CCSGG 1 cut(s) 200
BsaBI GATNNNNATC 2 cut(s) 231, 336
BsaHI GRCGYC 2 cut(s) 113, 158
BsaJI CCNNGG 3 cut(s) 213, 252, 574
Bsc4I CCNNNNNNNGG 1 cut(s) 259
Bse1I ACTGG 4 cut(s) 61, 175, 264, 456
Bse3DI GCAATG 1 cut(s) 359
Bse8I GATNNNNATC 2 cut(s) 231, 336
BseBI CCWGG 2 cut(s) 254, 576
BseDI CCNNGG 3 cut(s) 213, 252, 574
BseGI GGATG 1 cut(s) 827
BseJI GATNNNNATC 2 cut(s) 231, 336
BseLI CCNNNNNNNGG 1 cut(s) 259
BseMI GCAATG 1 cut(s) 359
BseMII CTCAG 3 cut(s) 182, 250, 517
BseNI ACTGG 4 cut(s) 61, 175, 264, 456
BseRI GAGGAG 1 cut(s) 208
BseSI GKGCMC 1 cut(s) 608
BseYI CCCAGC 1 cut(s) 692
BshFI GGCC 1 cut(s) 212
BsiHKAI GWGCWC 1 cut(s) 504
BsiSI CCGG 1 cut(s) 200
BslFI GGGAC 2 cut(s) 90, 462
BslI CCNNNNNNNGG 1 cut(s) 259
BsmAI GTCTC 2 cut(s) 568, 634
BsmBI CGTCTC 1 cut(s) 634
BsmFI GGGAC 2 cut(s) 90, 462
BsmI GAATGC 1 cut(s) 541
BsnI GGCC 1 cut(s) 212
Bsp1286I GDGCHC 3 cut(s) 200, 504, 608
Bsp1407I TGTACA 2 cut(s) 649, 721
Bsp143I GATC 2 cut(s) 226, 610
BspANI GGCC 1 cut(s) 212
BspCNI CTCAG 3 cut(s) 183, 249, 516
BspLI GGNNCC 4 cut(s) 7, 197, 354, 690
BspPI GGATC 1 cut(s) 605
BspQI GCTCTTC 1 cut(s) 307
BsrDI GCAATG 1 cut(s) 359
BsrGI TGTACA 2 cut(s) 649, 721
BsrI ACTGG 4 cut(s) 61, 175, 264, 456
BssECI CCNNGG 3 cut(s) 213, 252, 574
BssMI GATC 2 cut(s) 226, 610
BssNI GRCGYC 2 cut(s) 113, 158
BssSI CACGAG 1 cut(s) 206
BssT1I CCWWGG 1 cut(s) 213
Bst2BI CACGAG 1 cut(s) 206
Bst2UI CCWGG 2 cut(s) 254, 576
Bst4CI ACNGT 4 cut(s) 41, 714, 815, 926
Bst6I CTCTTC 3 cut(s) 142, 307, 571
BstACI GRCGYC 2 cut(s) 113, 158
BstAUI TGTACA 2 cut(s) 649, 721
BstC8I GCNNGC 1 cut(s) 414
BstDEI CTNAG 5 cut(s) 191, 236, 296, 503, 509
BstF5I GGATG 1 cut(s) 827
BstHHI GCGC 1 cut(s) 698
BstKTI GATC 2 cut(s) 229, 613
BstMAI GTCTC 2 cut(s) 568, 634
BstMBI GATC 2 cut(s) 226, 610
BstMWI GCNNNNNNNGC 2 cut(s) 164, 695
BstNI CCWGG 2 cut(s) 254, 576
BstNSI RCATGY 2 cut(s) 287, 770
BstSCI CCNGG 3 cut(s) 198, 252, 574
BstSFI CTRYAG 1 cut(s) 37
BstSLI GKGCMC 1 cut(s) 608
BstX2I RGATCY 1 cut(s) 610
BstYI RGATCY 1 cut(s) 610
BsuRI GGCC 1 cut(s) 212
BtsCI GGATG 1 cut(s) 827
BtsIMutI CAGTG 3 cut(s) 46, 581, 820
Cac8I GCNNGC 1 cut(s) 414
CaiI CAGNNNCTG 1 cut(s) 259
CfoI GCGC 1 cut(s) 698
Cfr13I GGNCC 3 cut(s) 202, 352, 578
CseI GACGC 2 cut(s) 121, 147
Csp6I GTAC 6 cut(s) 54, 314, 326, 650, 722, 907
CviAII CATG 7 cut(s) 266, 284, 370, 406, 725, 767, 869
CviQI GTAC 6 cut(s) 54, 314, 326, 650, 722, 907
DdeI CTNAG 5 cut(s) 191, 236, 296, 503, 509
DpnI GATC 2 cut(s) 228, 612
DpnII GATC 2 cut(s) 226, 610
Eam1104I CTCTTC 3 cut(s) 142, 307, 571
EarI CTCTTC 3 cut(s) 142, 307, 571
Ecl136II GAGCTC 1 cut(s) 502
Eco130I CCWWGG 1 cut(s) 213
Eco24I GRGCYC 2 cut(s) 200, 504
Eco47I GGWCC 3 cut(s) 202, 352, 578
Eco53kI GAGCTC 1 cut(s) 502
Eco57I CTGAAG 1 cut(s) 794
EcoICRI GAGCTC 1 cut(s) 502
EcoO109I RGGNCCY 1 cut(s) 352
EcoRII CCWGG 2 cut(s) 252, 574
EcoT14I CCWWGG 1 cut(s) 213
EcoT38I GRGCYC 2 cut(s) 200, 504
ErhI CCWWGG 1 cut(s) 213
Esp3I CGTCTC 1 cut(s) 634
FaeI CATG 7 cut(s) 269, 287, 373, 409, 728, 770, 872
FaqI GGGAC 2 cut(s) 90, 462
FatI CATG 7 cut(s) 265, 283, 369, 405, 724, 766, 868
FokI GGATG 1 cut(s) 834
FriOI GRGCYC 2 cut(s) 200, 504
FspBI CTAG 1 cut(s) 560
GlaI GCGC 1 cut(s) 697
GsaI CCCAGC 1 cut(s) 696
GsuI CTGGAG 1 cut(s) 781
HaeIII GGCC 1 cut(s) 212
HapII CCGG 1 cut(s) 200
HgaI GACGC 2 cut(s) 121, 147
HhaI GCGC 1 cut(s) 698
Hin1I GRCGYC 2 cut(s) 113, 158
Hin1II CATG 7 cut(s) 269, 287, 373, 409, 728, 770, 872
Hin6I GCGC 1 cut(s) 696
HinP1I GCGC 1 cut(s) 696
HincII GTYRAC 1 cut(s) 922
HindII GTYRAC 1 cut(s) 922
HinfI GANTC 5 cut(s) 337, 439, 668, 758, 794
HpaII CCGG 1 cut(s) 200
Hpy166II GTNNAC 4 cut(s) 289, 376, 907, 922
Hpy188I TCNGA 2 cut(s) 718, 774
Hpy8I GTNNAC 4 cut(s) 289, 376, 907, 922
Hpy99I CGWCG 1 cut(s) 115
HpyAV CCTTC 2 cut(s) 66, 875
HpyCH4III ACNGT 4 cut(s) 41, 714, 815, 926
HpyCH4IV ACGT 2 cut(s) 22, 478
HpyCH4V TGCA 3 cut(s) 62, 679, 766
HpyF10VI GCNNNNNNNGC 2 cut(s) 164, 695
HpyF3I CTNAG 5 cut(s) 191, 236, 296, 503, 509
HpySE526I ACGT 2 cut(s) 22, 478
Hsp92I GRCGYC 2 cut(s) 113, 158
Hsp92II CATG 7 cut(s) 269, 287, 373, 409, 728, 770, 872
HspAI GCGC 1 cut(s) 696
Kzo9I GATC 2 cut(s) 226, 610
LguI GCTCTTC 1 cut(s) 307
LmnI GCTCC 3 cut(s) 181, 195, 694
LweI GCATC 2 cut(s) 576, 640
MaeI CTAG 1 cut(s) 560
MaeII ACGT 2 cut(s) 22, 478
MaeIII GTNAC 3 cut(s) 106, 247, 809
MalI GATC 2 cut(s) 228, 612
MboI GATC 2 cut(s) 226, 610
MboII GAAGA 5 cut(s) 153, 156, 159, 294, 558
MflI RGATCY 1 cut(s) 610
MhlI GDGCHC 3 cut(s) 200, 504, 608
MluCI AATT 6 cut(s) 273, 752, 789, 864, 875, 891
MlyI GAGTC 1 cut(s) 788
MnlI CCTC 8 cut(s) 148, 186, 215, 303, 375, 429, 721, 811
MseI TTAA 5 cut(s) 177, 231, 495, 552, 621
MslI CAYNNNNRTG 1 cut(s) 783
MspI CCGG 1 cut(s) 200
MspR9I CCNGG 3 cut(s) 200, 254, 576
Mva1269I GAATGC 1 cut(s) 541
MvaI CCWGG 2 cut(s) 254, 576
MwoI GCNNNNNNNGC 2 cut(s) 164, 695
NciI CCSGG 1 cut(s) 200
NdeII GATC 2 cut(s) 226, 610
NlaIII CATG 7 cut(s) 269, 287, 373, 409, 728, 770, 872
NlaIV GGNNCC 4 cut(s) 7, 197, 354, 690
NmuCI GTSAC 1 cut(s) 809
NspI RCATGY 2 cut(s) 287, 770
PciI ACATGT 1 cut(s) 283
PciSI GCTCTTC 1 cut(s) 307
PctI GAATGC 1 cut(s) 541
PfeI GAWTC 4 cut(s) 337, 439, 668, 758
PflMI CCANNNNNTGG 1 cut(s) 259
PleI GAGTC 1 cut(s) 788
PpsI GAGTC 1 cut(s) 788
PpuMI RGGWCCY 1 cut(s) 352
PscI ACATGT 1 cut(s) 283
Psp124BI GAGCTC 1 cut(s) 504
Psp5II RGGWCCY 1 cut(s) 352
Psp6I CCWGG 2 cut(s) 252, 574
PspFI CCCAGC 1 cut(s) 692
PspGI CCWGG 2 cut(s) 252, 574
PspN4I GGNNCC 4 cut(s) 7, 197, 354, 690
PspPI GGNCC 3 cut(s) 202, 352, 578
PspPPI RGGWCCY 1 cut(s) 352
PstNI CAGNNNCTG 1 cut(s) 259
PsuI RGATCY 1 cut(s) 610
RsaI GTAC 6 cut(s) 55, 315, 327, 651, 723, 908
RsaNI GTAC 6 cut(s) 54, 314, 326, 650, 722, 907
RseI CAYNNNNRTG 1 cut(s) 783
SacI GAGCTC 1 cut(s) 504
SapI GCTCTTC 1 cut(s) 307
SaqAI TTAA 5 cut(s) 177, 231, 495, 552, 621
Sau3AI GATC 2 cut(s) 226, 610
Sau96I GGNCC 3 cut(s) 202, 352, 578
ScaI AGTACT 1 cut(s) 55
SchI GAGTC 1 cut(s) 788
ScrFI CCNGG 3 cut(s) 200, 254, 576
SduI GDGCHC 3 cut(s) 200, 504, 608
SfaNI GCATC 2 cut(s) 576, 640
SfcI CTRYAG 1 cut(s) 37
SinI GGWCC 3 cut(s) 202, 352, 578
SmiMI CAYNNNNRTG 1 cut(s) 783
SmlI CTYRAG 1 cut(s) 737
SmoI CTYRAG 1 cut(s) 737
SpeI ACTAGT 1 cut(s) 559
Sse9I AATT 6 cut(s) 273, 752, 789, 864, 875, 891
SspMI CTAG 1 cut(s) 560
SstI GAGCTC 1 cut(s) 504
StyD4I CCNGG 3 cut(s) 198, 252, 574
StyI CCWWGG 1 cut(s) 213
TaaI ACNGT 4 cut(s) 41, 714, 815, 926
TaiI ACGT 2 cut(s) 25, 481
TasI AATT 6 cut(s) 273, 752, 789, 864, 875, 891
TatI WGTACW 3 cut(s) 53, 649, 721
TfiI GAWTC 4 cut(s) 337, 439, 668, 758
Tru1I TTAA 5 cut(s) 177, 231, 495, 552, 621
Tru9I TTAA 5 cut(s) 177, 231, 495, 552, 621
TscAI CASTG 3 cut(s) 46, 588, 820
TseFI GTSAC 1 cut(s) 809
Tsp45I GTSAC 1 cut(s) 809
TspDTI ATGAA 2 cut(s) 801, 857
TspGWI ACGGA 2 cut(s) 603, 869
TspRI CASTG 3 cut(s) 46, 588, 820
Van91I CCANNNNNTGG 1 cut(s) 259
VpaK11BI GGWCC 3 cut(s) 202, 352, 578
XapI RAATTY 3 cut(s) 864, 875, 891
XceI RCATGY 2 cut(s) 287, 770
XspI CTAG 1 cut(s) 560
ZrmI AGTACT 1 cut(s) 55
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.