Rw1G014840

tRNA (guanine-N(7)-)-methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0006250
Physical Location & Seq
Forward (+)
33118298 .. 33127154
8857 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G014840.1

Sequence Viewer

Length: 309 bp
ATGTCATGCCTTACAACGTGCTTATTTCTGGGATGTGTGAAGTGCCGGAGTTGTGTGATGCTTGTCAAACGTGCTGATGTCTGGGTTAAAGAGCTGGGTCTTAGTAACATACATTTCATGTATGCAAATGCCACTACTTCATTTAATCAACTGGTGTCTACCTACCCTGGACCACTGATGCTTGTTTCAATCCTGTGCCCAGATCCGTATTTTAAGAGAAAGCATCACAAGAAACGGGTTGTACAGAAGCCTTTAGTAGATTCCATTGTGAATAATTTAGCTCCCCGCGGACAGGTTTGTCAACTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_4

Protein Analysis

102

Amino Acids

11.51

Weight (kDa)

9.5

Isoelectric Point (pI)

45.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_4 PF02390 23 - 99 1.9e-14 Putative methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 297
AccI GTMKAC 1 cut(s) 158
AccII CGCG 1 cut(s) 288
AciI CCGC 2 cut(s) 286, 288
AclWI GGATC 1 cut(s) 197
AfaI GTAC 1 cut(s) 243
AfiI CCNNNNNNNGG 1 cut(s) 292
AgsI TTSAA 1 cut(s) 189
AjnI CCWGG 1 cut(s) 166
AluBI AGCT 2 cut(s) 94, 281
AluI AGCT 2 cut(s) 94, 281
AlwI GGATC 1 cut(s) 197
AspS9I GGNCC 1 cut(s) 170
AvaII GGWCC 1 cut(s) 170
BaeGI GKGCMC 1 cut(s) 200
BciT130I CCWGG 1 cut(s) 168
BfmI CTRYAG 1 cut(s) 305
Bme1390I CCNGG 1 cut(s) 168
Bme18I GGWCC 1 cut(s) 170
BmgT120I GGNCC 1 cut(s) 170
BmrFI CCNGG 1 cut(s) 168
BmsI GCATC 3 cut(s) 48, 168, 232
BsaJI CCNNGG 2 cut(s) 166, 286
Bsc4I CCNNNNNNNGG 1 cut(s) 292
Bse1I ACTGG 1 cut(s) 156
BseBI CCWGG 1 cut(s) 168
BseDI CCNNGG 2 cut(s) 166, 286
BseGI GGATG 1 cut(s) 38
BseLI CCNNNNNNNGG 1 cut(s) 292
BseNI ACTGG 1 cut(s) 156
BseSI GKGCMC 1 cut(s) 200
BseYI CCCAGC 1 cut(s) 94
Bsh1236I CGCG 1 cut(s) 288
BsiSI CCGG 1 cut(s) 46
BslI CCNNNNNNNGG 1 cut(s) 292
Bsp1286I GDGCHC 1 cut(s) 200
Bsp1407I TGTACA 1 cut(s) 241
Bsp143I GATC 1 cut(s) 202
BspACI CCGC 2 cut(s) 286, 288
BspFNI CGCG 1 cut(s) 288
BspPI GGATC 1 cut(s) 197
BsrGI TGTACA 1 cut(s) 241
BsrI ACTGG 1 cut(s) 156
BssECI CCNNGG 2 cut(s) 166, 286
BssMI GATC 1 cut(s) 202
Bst2UI CCWGG 1 cut(s) 168
BstAUI TGTACA 1 cut(s) 241
BstDEI CTNAG 1 cut(s) 101
BstDSI CCRYGG 1 cut(s) 286
BstF5I GGATG 1 cut(s) 38
BstFNI CGCG 1 cut(s) 288
BstKTI GATC 1 cut(s) 205
BstMBI GATC 1 cut(s) 202
BstNI CCWGG 1 cut(s) 168
BstSCI CCNGG 1 cut(s) 166
BstSFI CTRYAG 1 cut(s) 305
BstSLI GKGCMC 1 cut(s) 200
BstUI CGCG 1 cut(s) 288
BstX2I RGATCY 1 cut(s) 202
BstYI RGATCY 1 cut(s) 202
BtgI CCRYGG 1 cut(s) 286
BtsCI GGATG 1 cut(s) 38
BtsIMutI CAGTG 1 cut(s) 173
Cfr13I GGNCC 1 cut(s) 170
Cfr42I CCGCGG 1 cut(s) 289
Csp6I GTAC 1 cut(s) 242
CviAII CATG 2 cut(s) 6, 118
CviJI RGCY 3 cut(s) 94, 250, 281
CviKI_1 RGCY 3 cut(s) 94, 250, 281
CviQI GTAC 1 cut(s) 242
DdeI CTNAG 1 cut(s) 101
DpnI GATC 1 cut(s) 204
DpnII GATC 1 cut(s) 202
DrdI GACNNNNNNGTC 1 cut(s) 297
DseDI GACNNNNNNGTC 1 cut(s) 297
Eco47I GGWCC 1 cut(s) 170
EcoRII CCWGG 1 cut(s) 166
FaeI CATG 2 cut(s) 9, 121
FaiI YATR 5 cut(s) 7, 110, 119, 123, 307
FatI CATG 2 cut(s) 5, 117
FauI CCCGC 1 cut(s) 293
FblI GTMKAC 1 cut(s) 158
FokI GGATG 1 cut(s) 45
GsaI CCCAGC 1 cut(s) 98
HapII CCGG 1 cut(s) 46
Hin1II CATG 2 cut(s) 9, 121
HincII GTYRAC 1 cut(s) 302
HindII GTYRAC 1 cut(s) 302
HinfI GANTC 1 cut(s) 260
HpaII CCGG 1 cut(s) 46
Hpy166II GTNNAC 2 cut(s) 159, 302
Hpy8I GTNNAC 2 cut(s) 159, 302
HpyCH4IV ACGT 2 cut(s) 17, 70
HpyCH4V TGCA 1 cut(s) 125
HpyF3I CTNAG 1 cut(s) 101
HpySE526I ACGT 2 cut(s) 17, 70
Hsp92II CATG 2 cut(s) 9, 121
KspI CCGCGG 1 cut(s) 289
Kzo9I GATC 1 cut(s) 202
LmnI GCTCC 1 cut(s) 286
LweI GCATC 3 cut(s) 48, 168, 232
MaeII ACGT 2 cut(s) 17, 70
MaeIII GTNAC 1 cut(s) 104
MalI GATC 1 cut(s) 204
MboI GATC 1 cut(s) 202
MflI RGATCY 1 cut(s) 202
MhlI GDGCHC 1 cut(s) 200
MluCI AATT 1 cut(s) 274
MseI TTAA 3 cut(s) 87, 144, 213
MspA1I CMGCKG 1 cut(s) 288
MspI CCGG 1 cut(s) 46
MspR9I CCNGG 1 cut(s) 168
MvaI CCWGG 1 cut(s) 168
MvnI CGCG 1 cut(s) 288
NdeII GATC 1 cut(s) 202
NlaIII CATG 2 cut(s) 9, 121
PfeI GAWTC 1 cut(s) 260
Psp6I CCWGG 1 cut(s) 166
PspFI CCCAGC 1 cut(s) 94
PspGI CCWGG 1 cut(s) 166
PspPI GGNCC 1 cut(s) 170
PsuI RGATCY 1 cut(s) 202
RsaI GTAC 1 cut(s) 243
RsaNI GTAC 1 cut(s) 242
SacII CCGCGG 1 cut(s) 289
SaqAI TTAA 3 cut(s) 87, 144, 213
Sau3AI GATC 1 cut(s) 202
Sau96I GGNCC 1 cut(s) 170
ScrFI CCNGG 1 cut(s) 168
SduI GDGCHC 1 cut(s) 200
SetI ASST 6 cut(s) 20, 73, 96, 164, 283, 297
SfaNI GCATC 3 cut(s) 48, 168, 232
SfcI CTRYAG 1 cut(s) 305
Sfr303I CCGCGG 1 cut(s) 289
SgrBI CCGCGG 1 cut(s) 289
SinI GGWCC 1 cut(s) 170
Sse9I AATT 1 cut(s) 274
SsiI CCGC 2 cut(s) 286, 288
StyD4I CCNGG 1 cut(s) 166
TaiI ACGT 2 cut(s) 20, 73
TasI AATT 1 cut(s) 274
TatI WGTACW 1 cut(s) 241
TfiI GAWTC 1 cut(s) 260
Tru1I TTAA 3 cut(s) 87, 144, 213
Tru9I TTAA 3 cut(s) 87, 144, 213
TscAI CASTG 1 cut(s) 180
TspDTI ATGAA 2 cut(s) 106, 129
TspGWI ACGGA 1 cut(s) 195
TspRI CASTG 1 cut(s) 180
VpaK11BI GGWCC 1 cut(s) 170
XmiI GTMKAC 1 cut(s) 158
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.