Rroxscaffold_4G00311200

tRNA (guanine-N(7)-)-methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0006250
Physical Location & Seq
Reverse (-)
33504505 .. 33510475
5971 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00311200.1

Sequence Viewer

Length: 834 bp
ATGGTCTGTTTAACGGCGCCGTTTTACTGGAACAGAAGAAGAGTGGAGGCCTGTGTTTCTACTGGGTTTAAGGAACAAGAGCTGAGGAGCCCGGACCTTGTGGCCTTGGAATATGCTGATCTTAATCTCACCCACAATGTTTCCCAGGAACTGGGTCACGTCAGAATTAGGCAACATGTGAATCCTCTTAGCTCTTCTTTATCTGCGCCTGTGGAAGTGCCTGATTGGAATCATGTGTTCAGGGACCCAACATTGCCGCTCATGGTGGACATTGGAAGTGGTAGTGGTAGATTTCTCATGTGGCTGGCGAAAAGAAATTTAGTTGTGAGGAATTATTTGGGACTGGAAATAAGGCAGAAACTTGTCAAACGTGCTGATGTCTGGGTTAAAGAGCTGGGTCTTAGTAATATACATTTCATGTATGCAAATGCCACTACTTCATTTACTCAACTGGTGTCTACCTACCCTGGACCACTGATGCTTGTTTCAATCCTGTGCCCAGATCCGTATTTTAAGAGAAAGCATCACAAGAGACGGGTTGTACAGAAGCCTTTAGTAGATTCCATTGTGAATTATTTAGCTCCCCACGGACAGGTGTTTATACAGTCCGATGTACTTGACGTTGCACTTGAGATGAGAAACCAATTTGATTCTCTGCCCCATGTTCTGAAGCACATAGATGAAATCGAATCCAGCGTGTTGTGTGACAGTGAGGGATGGTTGTTGAGCAATCCAATGGGCAAAAGGACGGAAAGAGAAATTCATGCTGAATTTGAAGGGGCAAAAATTTACAGGAGAGTGTACCAGAAGCGAGTACAGAAGGCTCTTGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_4

Protein Analysis

277

Amino Acids

31.84

Weight (kDa)

9.12

Isoelectric Point (pI)

58.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_4 PF02390 86 - 223 3.2e-31 Putative methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 16
AccB7I CCANNNNNTGG 1 cut(s) 151
AccBSI CCGCTC 1 cut(s) 259
AccI GTMKAC 1 cut(s) 458
AciI CCGC 1 cut(s) 257
AclWI GGATC 1 cut(s) 497
AcsI RAATTY 4 cut(s) 316, 759, 770, 786
AcuI CTGAAG 1 cut(s) 689
AcyI GRCGYC 1 cut(s) 17
AfaI GTAC 4 cut(s) 543, 615, 803, 816
AfiI CCNNNNNNNGG 2 cut(s) 151, 592
AflIII ACRYGT 1 cut(s) 175
AgsI TTSAA 2 cut(s) 489, 776
AjiI CACGTC 1 cut(s) 160
AjnI CCWGG 2 cut(s) 144, 466
AluBI AGCT 4 cut(s) 82, 192, 394, 581
AluI AGCT 4 cut(s) 82, 192, 394, 581
Alw26I GTCTC 1 cut(s) 526
AlwI GGATC 1 cut(s) 497
AlwNI CAGNNNCTG 1 cut(s) 151
AoxI GGCC 2 cut(s) 48, 102
ApoI RAATTY 4 cut(s) 316, 759, 770, 786
AspLEI GCGC 2 cut(s) 19, 208
AspS9I GGNCC 3 cut(s) 94, 244, 470
AsuC2I CCSGG 1 cut(s) 92
AsuHPI GGTGA 1 cut(s) 121
AvaII GGWCC 3 cut(s) 94, 244, 470
BaeGI GKGCMC 1 cut(s) 500
BanI GGYRCC 1 cut(s) 16
BanII GRGCYC 1 cut(s) 92
BbvCI CCTCAGC 1 cut(s) 83
BccI CCATC 1 cut(s) 711
BceAI ACGGC 2 cut(s) 4, 30
BciT130I CCWGG 2 cut(s) 146, 468
BcnI CCSGG 1 cut(s) 92
BcoDI GTCTC 1 cut(s) 526
BfoI RGCGCY 1 cut(s) 20
BisI GCNGC 1 cut(s) 257
BlsI GCNGC 1 cut(s) 258
Bme1390I CCNGG 3 cut(s) 92, 146, 468
Bme18I GGWCC 3 cut(s) 94, 244, 470
BmgBI CACGTC 1 cut(s) 160
BmgT120I GGNCC 3 cut(s) 94, 244, 470
BmiI GGNNCC 4 cut(s) 18, 89, 245, 246
BmrFI CCNGG 3 cut(s) 92, 146, 468
BmrI ACTGGG 2 cut(s) 72, 161
BmsI GCATC 2 cut(s) 468, 532
BmuI ACTGGG 2 cut(s) 72, 161
Bpu10I CCTNAGC 1 cut(s) 83
BpuEI CTTGAG 1 cut(s) 650
BpuMI CCSGG 1 cut(s) 92
BsaBI GATNNNNATC 2 cut(s) 123, 228
BsaHI GRCGYC 1 cut(s) 17
BsaJI CCNNGG 4 cut(s) 105, 144, 466, 586
Bsc4I CCNNNNNNNGG 2 cut(s) 151, 592
Bse1I ACTGG 5 cut(s) 32, 67, 156, 348, 456
Bse3DI GCAATG 1 cut(s) 251
Bse8I GATNNNNATC 2 cut(s) 123, 228
BseBI CCWGG 2 cut(s) 146, 468
BseDI CCNNGG 4 cut(s) 105, 144, 466, 586
BseGI GGATG 1 cut(s) 722
BseJI GATNNNNATC 2 cut(s) 123, 228
BseLI CCNNNNNNNGG 2 cut(s) 151, 592
BseMI GCAATG 1 cut(s) 251
BseMII CTCAG 1 cut(s) 74
BseNI ACTGG 5 cut(s) 32, 67, 156, 348, 456
BseRI GAGGAG 1 cut(s) 100
BseSI GKGCMC 1 cut(s) 500
BseYI CCCAGC 1 cut(s) 394
BshFI GGCC 2 cut(s) 50, 104
BshNI GGYRCC 1 cut(s) 16
BsiSI CCGG 1 cut(s) 92
BslFI GGGAC 2 cut(s) 257, 354
BslI CCNNNNNNNGG 2 cut(s) 151, 592
BsmAI GTCTC 1 cut(s) 526
BsmBI CGTCTC 1 cut(s) 526
BsmFI GGGAC 2 cut(s) 257, 354
BsnI GGCC 2 cut(s) 50, 104
Bsp1286I GDGCHC 2 cut(s) 92, 500
Bsp1407I TGTACA 1 cut(s) 541
Bsp143I GATC 2 cut(s) 118, 502
BspACI CCGC 1 cut(s) 257
BspANI GGCC 2 cut(s) 50, 104
BspCNI CTCAG 1 cut(s) 75
BspLI GGNNCC 4 cut(s) 18, 89, 245, 246
BspPI GGATC 1 cut(s) 497
BspQI GCTCTTC 1 cut(s) 199
BspT107I GGYRCC 1 cut(s) 16
BsrBI CCGCTC 1 cut(s) 259
BsrDI GCAATG 1 cut(s) 251
BsrGI TGTACA 1 cut(s) 541
BsrI ACTGG 5 cut(s) 32, 67, 156, 348, 456
BssECI CCNNGG 4 cut(s) 105, 144, 466, 586
BssMI GATC 2 cut(s) 118, 502
BssNI GRCGYC 1 cut(s) 17
BssT1I CCWWGG 1 cut(s) 105
Bst2UI CCWGG 2 cut(s) 146, 468
Bst4CI ACNGT 2 cut(s) 606, 710
Bst6I CTCTTC 2 cut(s) 34, 199
BstACI GRCGYC 1 cut(s) 17
BstAUI TGTACA 1 cut(s) 541
BstC8I GCNNGC 1 cut(s) 306
BstDEI CTNAG 3 cut(s) 83, 188, 401
BstDSI CCRYGG 1 cut(s) 586
BstF5I GGATG 1 cut(s) 722
BstH2I RGCGCY 1 cut(s) 20
BstHHI GCGC 2 cut(s) 19, 208
BstKTI GATC 2 cut(s) 121, 505
BstMAI GTCTC 1 cut(s) 526
BstMBI GATC 2 cut(s) 118, 502
BstNI CCWGG 2 cut(s) 146, 468
BstNSI RCATGY 1 cut(s) 179
BstSCI CCNGG 3 cut(s) 90, 144, 466
BstSLI GKGCMC 1 cut(s) 500
BstX2I RGATCY 1 cut(s) 502
BstYI RGATCY 1 cut(s) 502
BsuRI GGCC 2 cut(s) 50, 104
BtgI CCRYGG 1 cut(s) 586
BtrI CACGTC 1 cut(s) 160
BtsCI GGATG 1 cut(s) 722
BtsIMutI CAGTG 2 cut(s) 473, 715
Cac8I GCNNGC 1 cut(s) 306
CaiI CAGNNNCTG 1 cut(s) 151
CfoI GCGC 2 cut(s) 19, 208
Cfr13I GGNCC 3 cut(s) 94, 244, 470
Csp6I GTAC 4 cut(s) 542, 614, 802, 815
CviAII CATG 7 cut(s) 176, 233, 262, 298, 418, 662, 764
CviQI GTAC 4 cut(s) 542, 614, 802, 815
DdeI CTNAG 3 cut(s) 83, 188, 401
DinI GGCGCC 1 cut(s) 18
DpnI GATC 2 cut(s) 120, 504
DpnII GATC 2 cut(s) 118, 502
Eam1104I CTCTTC 2 cut(s) 34, 199
EarI CTCTTC 2 cut(s) 34, 199
Eco130I CCWWGG 1 cut(s) 105
Eco147I AGGCCT 1 cut(s) 50
Eco24I GRGCYC 1 cut(s) 92
Eco47I GGWCC 3 cut(s) 94, 244, 470
Eco57I CTGAAG 1 cut(s) 689
EcoO109I RGGNCCY 1 cut(s) 244
EcoRII CCWGG 2 cut(s) 144, 466
EcoT14I CCWWGG 1 cut(s) 105
EcoT38I GRGCYC 1 cut(s) 92
EgeI GGCGCC 1 cut(s) 18
EheI GGCGCC 1 cut(s) 18
ErhI CCWWGG 1 cut(s) 105
Esp3I CGTCTC 1 cut(s) 526
FaeI CATG 7 cut(s) 179, 236, 265, 301, 421, 665, 767
FaqI GGGAC 2 cut(s) 257, 354
FatI CATG 7 cut(s) 175, 232, 261, 297, 417, 661, 763
FblI GTMKAC 1 cut(s) 458
Fnu4HI GCNGC 1 cut(s) 257
FokI GGATG 1 cut(s) 729
FriOI GRGCYC 1 cut(s) 92
Fsp4HI GCNGC 1 cut(s) 257
GlaI GCGC 2 cut(s) 18, 207
GluI GCNGC 1 cut(s) 257
GsaI CCCAGC 1 cut(s) 398
HaeII RGCGCY 1 cut(s) 20
HaeIII GGCC 2 cut(s) 50, 104
HapII CCGG 1 cut(s) 92
HhaI GCGC 2 cut(s) 19, 208
Hin1I GRCGYC 1 cut(s) 17
Hin1II CATG 7 cut(s) 179, 236, 265, 301, 421, 665, 767
Hin6I GCGC 2 cut(s) 17, 206
HinP1I GCGC 2 cut(s) 17, 206
HinfI GANTC 5 cut(s) 181, 229, 560, 650, 689
HpaII CCGG 1 cut(s) 92
HphI GGTGA 1 cut(s) 121
Hpy166II GTNNAC 3 cut(s) 268, 459, 802
Hpy188I TCNGA 3 cut(s) 164, 610, 669
Hpy8I GTNNAC 3 cut(s) 268, 459, 802
HpyAV CCTTC 2 cut(s) 770, 814
HpyCH4III ACNGT 2 cut(s) 606, 710
HpyCH4IV ACGT 3 cut(s) 159, 370, 621
HpyCH4V TGCA 2 cut(s) 425, 626
HpyF3I CTNAG 3 cut(s) 83, 188, 401
HpySE526I ACGT 3 cut(s) 159, 370, 621
Hsp92I GRCGYC 1 cut(s) 17
Hsp92II CATG 7 cut(s) 179, 236, 265, 301, 421, 665, 767
HspAI GCGC 2 cut(s) 17, 206
KasI GGCGCC 1 cut(s) 16
KflI GGGWCCC 1 cut(s) 244
Kzo9I GATC 2 cut(s) 118, 502
LguI GCTCTTC 1 cut(s) 199
LmnI GCTCC 2 cut(s) 87, 586
LweI GCATC 2 cut(s) 468, 532
MaeII ACGT 3 cut(s) 159, 370, 621
MaeIII GTNAC 2 cut(s) 155, 704
MalI GATC 2 cut(s) 120, 504
MbiI CCGCTC 1 cut(s) 259
MboI GATC 2 cut(s) 118, 502
MboII GAAGA 3 cut(s) 48, 51, 186
MflI RGATCY 1 cut(s) 502
MhlI GDGCHC 2 cut(s) 92, 500
MluCI AATT 8 cut(s) 165, 316, 331, 571, 644, 759, 770, 786
Mly113I GGCGCC 1 cut(s) 17
MnlI CCTC 5 cut(s) 40, 78, 195, 321, 706
MseI TTAA 5 cut(s) 11, 69, 123, 387, 513
MslI CAYNNNNRTG 1 cut(s) 678
MspI CCGG 1 cut(s) 92
MspR9I CCNGG 3 cut(s) 92, 146, 468
MvaI CCWGG 2 cut(s) 146, 468
NarI GGCGCC 1 cut(s) 17
NciI CCSGG 1 cut(s) 92
NdeII GATC 2 cut(s) 118, 502
NlaIII CATG 7 cut(s) 179, 236, 265, 301, 421, 665, 767
NlaIV GGNNCC 4 cut(s) 18, 89, 245, 246
NmuCI GTSAC 2 cut(s) 155, 704
NspI RCATGY 1 cut(s) 179
PceI AGGCCT 1 cut(s) 50
PciI ACATGT 1 cut(s) 175
PciSI GCTCTTC 1 cut(s) 199
PcsI WCGNNNNNNNCGW 1 cut(s) 693
PfeI GAWTC 5 cut(s) 181, 229, 560, 650, 689
PflMI CCANNNNNTGG 1 cut(s) 151
PkrI GCNGC 1 cut(s) 258
PluTI GGCGCC 1 cut(s) 20
PpuMI RGGWCCY 1 cut(s) 244
PscI ACATGT 1 cut(s) 175
Psp5II RGGWCCY 1 cut(s) 244
Psp6I CCWGG 2 cut(s) 144, 466
PspFI CCCAGC 1 cut(s) 394
PspGI CCWGG 2 cut(s) 144, 466
PspN4I GGNNCC 4 cut(s) 18, 89, 245, 246
PspPI GGNCC 3 cut(s) 94, 244, 470
PspPPI RGGWCCY 1 cut(s) 244
PstNI CAGNNNCTG 1 cut(s) 151
PsuI RGATCY 1 cut(s) 502
RsaI GTAC 4 cut(s) 543, 615, 803, 816
RsaNI GTAC 4 cut(s) 542, 614, 802, 815
RseI CAYNNNNRTG 1 cut(s) 678
SapI GCTCTTC 1 cut(s) 199
SaqAI TTAA 5 cut(s) 11, 69, 123, 387, 513
SatI GCNGC 1 cut(s) 257
Sau3AI GATC 2 cut(s) 118, 502
Sau96I GGNCC 3 cut(s) 94, 244, 470
ScrFI CCNGG 3 cut(s) 92, 146, 468
SduI GDGCHC 2 cut(s) 92, 500
SfaNI GCATC 2 cut(s) 468, 532
SfoI GGCGCC 1 cut(s) 18
SinI GGWCC 3 cut(s) 94, 244, 470
SmiMI CAYNNNNRTG 1 cut(s) 678
SmlI CTYRAG 1 cut(s) 629
SmoI CTYRAG 1 cut(s) 629
Sse9I AATT 8 cut(s) 165, 316, 331, 571, 644, 759, 770, 786
SseBI AGGCCT 1 cut(s) 50
SsiI CCGC 1 cut(s) 257
SspDI GGCGCC 1 cut(s) 16
StuI AGGCCT 1 cut(s) 50
StyD4I CCNGG 3 cut(s) 90, 144, 466
StyI CCWWGG 1 cut(s) 105
TaaI ACNGT 2 cut(s) 606, 710
TaiI ACGT 3 cut(s) 162, 373, 624
TaqI TCGA 1 cut(s) 687
TasI AATT 8 cut(s) 165, 316, 331, 571, 644, 759, 770, 786
TatI WGTACW 3 cut(s) 541, 613, 814
TauI GCSGC 1 cut(s) 259
TfiI GAWTC 5 cut(s) 181, 229, 560, 650, 689
Tru1I TTAA 5 cut(s) 11, 69, 123, 387, 513
Tru9I TTAA 5 cut(s) 11, 69, 123, 387, 513
TscAI CASTG 2 cut(s) 480, 715
TseFI GTSAC 2 cut(s) 155, 704
Tsp45I GTSAC 2 cut(s) 155, 704
TspDTI ATGAA 4 cut(s) 406, 429, 696, 752
TspGWI ACGGA 3 cut(s) 495, 603, 764
TspRI CASTG 2 cut(s) 480, 715
Van91I CCANNNNNTGG 1 cut(s) 151
VpaK11BI GGWCC 3 cut(s) 94, 244, 470
XapI RAATTY 4 cut(s) 316, 759, 770, 786
XceI RCATGY 1 cut(s) 179
XmiI GTMKAC 1 cut(s) 458
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.