Rw1G014880

tRNA (guanine-N(7)-)-methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0006250
Physical Location & Seq
Forward (+)
33194131 .. 33196421
2291 bp
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UTR
Exon/CDS
Intron
Rw1G014880.1

Sequence Viewer

Length: 312 bp
ATGCTTGTCAAACGTGCTGATGTCTGGGTTAAAGAGCTGGGTCTTAGTAACATACATTTCATGTATGCAAATGCCACTACTTCATTTAATCAACTGGTGTCTGCCTACCCTGGACCACTGATGCTTGTTTCAATCCTGTGCCCAGATCCGTATTTTAAGAGAAAGCATCACAAGAGACGGGTTGTACAGAAGCCGTTTATAGATTCCATTGTGAATAATTTAGCTCCCCGCGGACAGGTGTTTATACAGTCCGATGTACTTGACGTCGCACTTGAGATGAGAAACCAATTTGATTCTCTGCCCCATAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_4

Protein Analysis

103

Amino Acids

11.88

Weight (kDa)

9.73

Isoelectric Point (pI)

46.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_4 PF02390 10 - 100 6.4e-20 Putative methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 267
AccII CGCG 1 cut(s) 231
AciI CCGC 2 cut(s) 229, 231
AclWI GGATC 1 cut(s) 140
AcyI GRCGYC 1 cut(s) 264
AfaI GTAC 2 cut(s) 186, 258
AfiI CCNNNNNNNGG 1 cut(s) 235
AgsI TTSAA 1 cut(s) 132
AjnI CCWGG 1 cut(s) 109
AluBI AGCT 2 cut(s) 37, 224
AluI AGCT 2 cut(s) 37, 224
Alw26I GTCTC 1 cut(s) 169
AlwI GGATC 1 cut(s) 140
AspS9I GGNCC 1 cut(s) 113
AvaII GGWCC 1 cut(s) 113
BaeGI GKGCMC 1 cut(s) 143
BceAI ACGGC 1 cut(s) 178
BciT130I CCWGG 1 cut(s) 111
BcoDI GTCTC 1 cut(s) 169
Bme1390I CCNGG 1 cut(s) 111
Bme18I GGWCC 1 cut(s) 113
BmgT120I GGNCC 1 cut(s) 113
BmrFI CCNGG 1 cut(s) 111
BmsI GCATC 2 cut(s) 111, 175
BpuEI CTTGAG 1 cut(s) 293
BsaHI GRCGYC 1 cut(s) 264
BsaJI CCNNGG 2 cut(s) 109, 229
Bsc4I CCNNNNNNNGG 1 cut(s) 235
Bse1I ACTGG 1 cut(s) 99
BseBI CCWGG 1 cut(s) 111
BseDI CCNNGG 2 cut(s) 109, 229
BseLI CCNNNNNNNGG 1 cut(s) 235
BseNI ACTGG 1 cut(s) 99
BseSI GKGCMC 1 cut(s) 143
BseYI CCCAGC 1 cut(s) 37
Bsh1236I CGCG 1 cut(s) 231
BslI CCNNNNNNNGG 1 cut(s) 235
BsmAI GTCTC 1 cut(s) 169
BsmBI CGTCTC 1 cut(s) 169
Bsp1286I GDGCHC 1 cut(s) 143
Bsp1407I TGTACA 1 cut(s) 184
Bsp143I GATC 1 cut(s) 145
BspACI CCGC 2 cut(s) 229, 231
BspFNI CGCG 1 cut(s) 231
BspPI GGATC 1 cut(s) 140
BsrGI TGTACA 1 cut(s) 184
BsrI ACTGG 1 cut(s) 99
BssECI CCNNGG 2 cut(s) 109, 229
BssMI GATC 1 cut(s) 145
BssNI GRCGYC 1 cut(s) 264
Bst2UI CCWGG 1 cut(s) 111
Bst4CI ACNGT 1 cut(s) 249
BstACI GRCGYC 1 cut(s) 264
BstAUI TGTACA 1 cut(s) 184
BstDEI CTNAG 1 cut(s) 44
BstDSI CCRYGG 1 cut(s) 229
BstFNI CGCG 1 cut(s) 231
BstKTI GATC 1 cut(s) 148
BstMAI GTCTC 1 cut(s) 169
BstMBI GATC 1 cut(s) 145
BstNI CCWGG 1 cut(s) 111
BstSCI CCNGG 1 cut(s) 109
BstSLI GKGCMC 1 cut(s) 143
BstUI CGCG 1 cut(s) 231
BstX2I RGATCY 1 cut(s) 145
BstYI RGATCY 1 cut(s) 145
BtgI CCRYGG 1 cut(s) 229
BtsIMutI CAGTG 1 cut(s) 116
Cfr13I GGNCC 1 cut(s) 113
Cfr42I CCGCGG 1 cut(s) 232
Csp6I GTAC 2 cut(s) 185, 257
CviAII CATG 1 cut(s) 61
CviJI RGCY 3 cut(s) 37, 193, 224
CviKI_1 RGCY 3 cut(s) 37, 193, 224
CviQI GTAC 2 cut(s) 185, 257
DdeI CTNAG 1 cut(s) 44
DpnI GATC 1 cut(s) 147
DpnII GATC 1 cut(s) 145
Eco47I GGWCC 1 cut(s) 113
EcoRII CCWGG 1 cut(s) 109
Esp3I CGTCTC 1 cut(s) 169
FaeI CATG 1 cut(s) 64
FaiI YATR 6 cut(s) 53, 62, 66, 200, 245, 306
FatI CATG 1 cut(s) 60
FauI CCCGC 1 cut(s) 236
GsaI CCCAGC 1 cut(s) 41
Hin1I GRCGYC 1 cut(s) 264
Hin1II CATG 1 cut(s) 64
HinfI GANTC 2 cut(s) 203, 293
Hpy188I TCNGA 1 cut(s) 253
Hpy99I CGWCG 1 cut(s) 269
HpyCH4III ACNGT 1 cut(s) 249
HpyCH4IV ACGT 2 cut(s) 13, 264
HpyCH4V TGCA 1 cut(s) 68
HpyF3I CTNAG 1 cut(s) 44
HpySE526I ACGT 2 cut(s) 13, 264
Hsp92I GRCGYC 1 cut(s) 264
Hsp92II CATG 1 cut(s) 64
KspI CCGCGG 1 cut(s) 232
Kzo9I GATC 1 cut(s) 145
LmnI GCTCC 1 cut(s) 229
LpnPI CCDG 8 cut(s) 10, 23, 80, 96, 123, 149, 156, 221
LweI GCATC 2 cut(s) 111, 175
MaeII ACGT 2 cut(s) 13, 264
MaeIII GTNAC 1 cut(s) 47
MalI GATC 1 cut(s) 147
MboI GATC 1 cut(s) 145
MflI RGATCY 1 cut(s) 145
MhlI GDGCHC 1 cut(s) 143
MluCI AATT 2 cut(s) 217, 287
MseI TTAA 3 cut(s) 30, 87, 156
MspA1I CMGCKG 1 cut(s) 231
MspR9I CCNGG 1 cut(s) 111
MvaI CCWGG 1 cut(s) 111
MvnI CGCG 1 cut(s) 231
NdeII GATC 1 cut(s) 145
NlaIII CATG 1 cut(s) 64
PfeI GAWTC 2 cut(s) 203, 293
Psp6I CCWGG 1 cut(s) 109
PspFI CCCAGC 1 cut(s) 37
PspGI CCWGG 1 cut(s) 109
PspPI GGNCC 1 cut(s) 113
PsuI RGATCY 1 cut(s) 145
RsaI GTAC 2 cut(s) 186, 258
RsaNI GTAC 2 cut(s) 185, 257
SacII CCGCGG 1 cut(s) 232
SaqAI TTAA 3 cut(s) 30, 87, 156
Sau3AI GATC 1 cut(s) 145
Sau96I GGNCC 1 cut(s) 113
ScrFI CCNGG 1 cut(s) 111
SduI GDGCHC 1 cut(s) 143
SetI ASST 5 cut(s) 16, 39, 226, 240, 267
SfaNI GCATC 2 cut(s) 111, 175
Sfr303I CCGCGG 1 cut(s) 232
SgrBI CCGCGG 1 cut(s) 232
SinI GGWCC 1 cut(s) 113
SmlI CTYRAG 1 cut(s) 272
SmoI CTYRAG 1 cut(s) 272
Sse9I AATT 2 cut(s) 217, 287
SsiI CCGC 2 cut(s) 229, 231
StyD4I CCNGG 1 cut(s) 109
TaaI ACNGT 1 cut(s) 249
TaiI ACGT 2 cut(s) 16, 267
TasI AATT 2 cut(s) 217, 287
TatI WGTACW 2 cut(s) 184, 256
TfiI GAWTC 2 cut(s) 203, 293
Tru1I TTAA 3 cut(s) 30, 87, 156
Tru9I TTAA 3 cut(s) 30, 87, 156
TscAI CASTG 1 cut(s) 123
TspDTI ATGAA 2 cut(s) 49, 72
TspGWI ACGGA 1 cut(s) 138
TspRI CASTG 1 cut(s) 123
VpaK11BI GGWCC 1 cut(s) 113
ZraI GACGTC 1 cut(s) 265
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.