Rh1CG164900

tRNA (guanine-N(7)-)-methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1C
OG0006250
Physical Location & Seq
Forward (+)
36379079 .. 36384685
5607 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1CG164900.1

Sequence Viewer

Length: 945 bp
ATGGGTTCTTGCGTCTGCTTACGTCAGCACTGCTGCTACGGTGGTTTGGGCGGTGCATTTCAAAGAAGGGGTCTTTGTAGTCTGTTCCGAAAAGGGGTTGTTGCCCCAGATTTAACGGCGCCGTTTTATCAGTATCACAGGAACAGAAGAAGAGTGGAGGCCTGTGTTTCTACTGGGTTTAAGGAACTGGAGCTGAGGAGCCCGGACCTTGTGGCCTTGGAATATGCTGATCTTAATCTCACCCACAATGTTTCCCAGGAACTGGGTCACGTCAGAATTAGGCAACATGTGAACCCTCTTAGCTCTTCTTTATCTGTGCCTGTGGAAGTGGCTGATTGGAATCAAGTGTTCAGGGACCCAACATTGCCGCTCATGGTGGACATTGGAAGTGGTAGTGGTAGATTTCTCATGTGGCTGGCGAAAAGAAACTTAGTTGTGAGGAATTATTTGGGACTGGAAATAAGGCAGAAACTTGTCAAACGTGCTGATGTCTGGGTTAAAGAGCTGGGTCTTAGTAACATACATTTCATGTATGCAAATGCCACTACTTCATTTAATCAACTGGTGTCTACCTACCCTGGACCACTGATGCTTGTTTCAATCCTGTGCCCAGATCCGTATTTTAAGAGAAAGCATCACAAGAGACGGGTTGTACAGAAGCCTTTAGTAGATTCCATTGTGAATAATTTAGCTCCCCGCGGACAGGTGTTTATACAGTCCGATGTACTTGACGTCGCACTTGAGATGAGAAACCAATTTGATTCTCTGCCCCATGTTCTGAAGCACATAGATGAAATCGAATCCAGCGTGTTGTGTGACAGTGAGGGATGGTTGTTGAGCAATCCAACGGGAAAAAGGACGGAAAGAGAAATTCATGCTGAATTTGAAGGGGCAAAAATTTACAGGAGAGTATACCAGAAGCAAGTACAGAAGGCTCTTGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_4

Protein Analysis

314

Amino Acids

35.74

Weight (kDa)

9.34

Isoelectric Point (pI)

53.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_4 PF02390 123 - 260 3.9e-31 Putative methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 735
AccB1I GGYRCC 1 cut(s) 118
AccB7I CCANNNNNTGG 1 cut(s) 262
AccBSI CCGCTC 1 cut(s) 370
AccI GTMKAC 2 cut(s) 569, 912
AccII CGCG 1 cut(s) 699
AciI CCGC 4 cut(s) 51, 368, 697, 699
AclWI GGATC 1 cut(s) 608
AcsI RAATTY 3 cut(s) 870, 881, 897
AcuI CTGAAG 1 cut(s) 800
AcyI GRCGYC 2 cut(s) 119, 732
AfaI GTAC 3 cut(s) 654, 726, 927
AfiI CCNNNNNNNGG 3 cut(s) 94, 262, 703
AflIII ACRYGT 1 cut(s) 286
AgsI TTSAA 3 cut(s) 62, 600, 887
AjiI CACGTC 1 cut(s) 271
AjnI CCWGG 2 cut(s) 255, 577
AluBI AGCT 4 cut(s) 193, 303, 505, 692
AluI AGCT 4 cut(s) 193, 303, 505, 692
Alw26I GTCTC 1 cut(s) 637
AlwI GGATC 1 cut(s) 608
AlwNI CAGNNNCTG 1 cut(s) 262
AoxI GGCC 2 cut(s) 159, 213
ApeKI GCWGC 1 cut(s) 33
ApoI RAATTY 3 cut(s) 870, 881, 897
ArsI GACNNNNNNTTYG 2 cut(s) 55, 87
AspLEI GCGC 1 cut(s) 121
AspS9I GGNCC 3 cut(s) 205, 355, 581
AsuC2I CCSGG 1 cut(s) 203
AsuHPI GGTGA 1 cut(s) 232
AvaII GGWCC 3 cut(s) 205, 355, 581
BaeGI GKGCMC 1 cut(s) 611
BanI GGYRCC 1 cut(s) 118
BanII GRGCYC 1 cut(s) 203
BbvCI CCTCAGC 1 cut(s) 194
BbvI GCAGC 1 cut(s) 20
BccI CCATC 1 cut(s) 822
BceAI ACGGC 2 cut(s) 106, 132
BciT130I CCWGG 2 cut(s) 257, 579
BcnI CCSGG 1 cut(s) 203
BcoDI GTCTC 1 cut(s) 637
BfoI RGCGCY 1 cut(s) 122
BisI GCNGC 2 cut(s) 34, 368
BlsI GCNGC 2 cut(s) 35, 369
Bme1390I CCNGG 3 cut(s) 203, 257, 579
Bme18I GGWCC 3 cut(s) 205, 355, 581
BmgBI CACGTC 1 cut(s) 271
BmgT120I GGNCC 3 cut(s) 205, 355, 581
BmiI GGNNCC 4 cut(s) 120, 200, 356, 357
BmrFI CCNGG 3 cut(s) 203, 257, 579
BmrI ACTGGG 2 cut(s) 183, 272
BmsI GCATC 2 cut(s) 579, 643
BmuI ACTGGG 2 cut(s) 183, 272
BpmI CTGGAG 1 cut(s) 209
Bpu10I CCTNAGC 1 cut(s) 194
BpuEI CTTGAG 1 cut(s) 761
BpuMI CCSGG 1 cut(s) 203
BsaBI GATNNNNATC 2 cut(s) 234, 339
BsaHI GRCGYC 2 cut(s) 119, 732
BsaJI CCNNGG 4 cut(s) 216, 255, 577, 697
Bsc4I CCNNNNNNNGG 3 cut(s) 94, 262, 703
Bse1I ACTGG 5 cut(s) 178, 192, 267, 459, 567
Bse3DI GCAATG 1 cut(s) 362
Bse8I GATNNNNATC 2 cut(s) 234, 339
BseBI CCWGG 2 cut(s) 257, 579
BseDI CCNNGG 4 cut(s) 216, 255, 577, 697
BseGI GGATG 1 cut(s) 833
BseJI GATNNNNATC 2 cut(s) 234, 339
BseLI CCNNNNNNNGG 3 cut(s) 94, 262, 703
BseMI GCAATG 1 cut(s) 362
BseMII CTCAG 1 cut(s) 185
BseNI ACTGG 5 cut(s) 178, 192, 267, 459, 567
BseRI GAGGAG 1 cut(s) 211
BseSI GKGCMC 1 cut(s) 611
BseXI GCAGC 1 cut(s) 20
BseYI CCCAGC 1 cut(s) 505
Bsh1236I CGCG 1 cut(s) 699
BshFI GGCC 2 cut(s) 161, 215
BshNI GGYRCC 1 cut(s) 118
BsiSI CCGG 1 cut(s) 203
BslFI GGGAC 2 cut(s) 368, 465
BslI CCNNNNNNNGG 3 cut(s) 94, 262, 703
BsmAI GTCTC 1 cut(s) 637
BsmBI CGTCTC 1 cut(s) 637
BsmFI GGGAC 2 cut(s) 368, 465
BsnI GGCC 2 cut(s) 161, 215
Bsp1286I GDGCHC 2 cut(s) 203, 611
Bsp1407I TGTACA 1 cut(s) 652
Bsp143I GATC 2 cut(s) 229, 613
BspACI CCGC 4 cut(s) 51, 368, 697, 699
BspANI GGCC 2 cut(s) 161, 215
BspCNI CTCAG 1 cut(s) 186
BspFNI CGCG 1 cut(s) 699
BspLI GGNNCC 4 cut(s) 120, 200, 356, 357
BspPI GGATC 1 cut(s) 608
BspQI GCTCTTC 1 cut(s) 310
BspT107I GGYRCC 1 cut(s) 118
BsrBI CCGCTC 1 cut(s) 370
BsrDI GCAATG 1 cut(s) 362
BsrGI TGTACA 1 cut(s) 652
BsrI ACTGG 5 cut(s) 178, 192, 267, 459, 567
BssECI CCNNGG 4 cut(s) 216, 255, 577, 697
BssMI GATC 2 cut(s) 229, 613
BssNAI GTATAC 1 cut(s) 913
BssNI GRCGYC 2 cut(s) 119, 732
BssT1I CCWWGG 1 cut(s) 216
Bst1107I GTATAC 1 cut(s) 913
Bst2UI CCWGG 2 cut(s) 257, 579
Bst4CI ACNGT 3 cut(s) 41, 717, 821
Bst6I CTCTTC 2 cut(s) 145, 310
BstACI GRCGYC 2 cut(s) 119, 732
BstAUI TGTACA 1 cut(s) 652
BstC8I GCNNGC 1 cut(s) 417
BstDEI CTNAG 4 cut(s) 194, 299, 430, 512
BstDSI CCRYGG 1 cut(s) 697
BstF5I GGATG 1 cut(s) 833
BstFNI CGCG 1 cut(s) 699
BstH2I RGCGCY 1 cut(s) 122
BstHHI GCGC 1 cut(s) 121
BstKTI GATC 2 cut(s) 232, 616
BstMAI GTCTC 1 cut(s) 637
BstMBI GATC 2 cut(s) 229, 613
BstNI CCWGG 2 cut(s) 257, 579
BstNSI RCATGY 1 cut(s) 290
BstSCI CCNGG 3 cut(s) 201, 255, 577
BstSLI GKGCMC 1 cut(s) 611
BstUI CGCG 1 cut(s) 699
BstV1I GCAGC 1 cut(s) 20
BstX2I RGATCY 1 cut(s) 613
BstYI RGATCY 1 cut(s) 613
BstZ17I GTATAC 1 cut(s) 913
BsuRI GGCC 2 cut(s) 161, 215
BtgI CCRYGG 1 cut(s) 697
BtrI CACGTC 1 cut(s) 271
BtsCI GGATG 1 cut(s) 833
BtsI GCAGTG 1 cut(s) 28
BtsIMutI CAGTG 3 cut(s) 28, 584, 826
Cac8I GCNNGC 1 cut(s) 417
CaiI CAGNNNCTG 1 cut(s) 262
CfoI GCGC 1 cut(s) 121
Cfr13I GGNCC 3 cut(s) 205, 355, 581
Cfr42I CCGCGG 1 cut(s) 700
Csp6I GTAC 3 cut(s) 653, 725, 926
CviAII CATG 6 cut(s) 287, 373, 409, 529, 773, 875
CviQI GTAC 3 cut(s) 653, 725, 926
DdeI CTNAG 4 cut(s) 194, 299, 430, 512
DinI GGCGCC 1 cut(s) 120
DpnI GATC 2 cut(s) 231, 615
DpnII GATC 2 cut(s) 229, 613
Eam1104I CTCTTC 2 cut(s) 145, 310
EarI CTCTTC 2 cut(s) 145, 310
Eco130I CCWWGG 1 cut(s) 216
Eco147I AGGCCT 1 cut(s) 161
Eco24I GRGCYC 1 cut(s) 203
Eco47I GGWCC 3 cut(s) 205, 355, 581
Eco57I CTGAAG 1 cut(s) 800
EcoO109I RGGNCCY 1 cut(s) 355
EcoRII CCWGG 2 cut(s) 255, 577
EcoT14I CCWWGG 1 cut(s) 216
EcoT38I GRGCYC 1 cut(s) 203
EgeI GGCGCC 1 cut(s) 120
EheI GGCGCC 1 cut(s) 120
ErhI CCWWGG 1 cut(s) 216
Esp3I CGTCTC 1 cut(s) 637
FaeI CATG 6 cut(s) 290, 376, 412, 532, 776, 878
FaqI GGGAC 2 cut(s) 368, 465
FatI CATG 6 cut(s) 286, 372, 408, 528, 772, 874
FauI CCCGC 1 cut(s) 704
FblI GTMKAC 2 cut(s) 569, 912
Fnu4HI GCNGC 2 cut(s) 34, 368
FokI GGATG 1 cut(s) 840
FriOI GRGCYC 1 cut(s) 203
Fsp4HI GCNGC 2 cut(s) 34, 368
GlaI GCGC 1 cut(s) 120
GluI GCNGC 2 cut(s) 34, 368
GsaI CCCAGC 1 cut(s) 509
GsuI CTGGAG 1 cut(s) 209
HaeII RGCGCY 1 cut(s) 122
HaeIII GGCC 2 cut(s) 161, 215
HapII CCGG 1 cut(s) 203
HhaI GCGC 1 cut(s) 121
Hin1I GRCGYC 2 cut(s) 119, 732
Hin1II CATG 6 cut(s) 290, 376, 412, 532, 776, 878
Hin6I GCGC 1 cut(s) 119
HinP1I GCGC 1 cut(s) 119
HinfI GANTC 4 cut(s) 340, 671, 761, 800
HpaII CCGG 1 cut(s) 203
HphI GGTGA 1 cut(s) 232
Hpy166II GTNNAC 4 cut(s) 292, 379, 570, 913
Hpy188I TCNGA 4 cut(s) 89, 275, 721, 780
Hpy8I GTNNAC 4 cut(s) 292, 379, 570, 913
Hpy99I CGWCG 1 cut(s) 737
HpyAV CCTTC 3 cut(s) 60, 881, 925
HpyCH4III ACNGT 3 cut(s) 41, 717, 821
HpyCH4IV ACGT 4 cut(s) 22, 270, 481, 732
HpyCH4V TGCA 2 cut(s) 56, 536
HpyF3I CTNAG 4 cut(s) 194, 299, 430, 512
HpySE526I ACGT 4 cut(s) 22, 270, 481, 732
Hsp92I GRCGYC 2 cut(s) 119, 732
Hsp92II CATG 6 cut(s) 290, 376, 412, 532, 776, 878
HspAI GCGC 1 cut(s) 119
KasI GGCGCC 1 cut(s) 118
KflI GGGWCCC 1 cut(s) 355
KspI CCGCGG 1 cut(s) 700
Kzo9I GATC 2 cut(s) 229, 613
LguI GCTCTTC 1 cut(s) 310
LmnI GCTCC 3 cut(s) 190, 198, 697
Lsp1109I GCAGC 1 cut(s) 20
LweI GCATC 2 cut(s) 579, 643
MaeII ACGT 4 cut(s) 22, 270, 481, 732
MaeIII GTNAC 3 cut(s) 266, 515, 815
MalI GATC 2 cut(s) 231, 615
MbiI CCGCTC 1 cut(s) 370
MboI GATC 2 cut(s) 229, 613
MboII GAAGA 3 cut(s) 159, 162, 297
MflI RGATCY 1 cut(s) 613
MhlI GDGCHC 2 cut(s) 203, 611
MluCI AATT 7 cut(s) 276, 442, 685, 755, 870, 881, 897
Mly113I GGCGCC 1 cut(s) 119
MmeI TCCRAC 1 cut(s) 869
MnlI CCTC 5 cut(s) 151, 189, 306, 432, 817
MseI TTAA 6 cut(s) 113, 180, 234, 498, 555, 624
MslI CAYNNNNRTG 1 cut(s) 789
MspA1I CMGCKG 1 cut(s) 699
MspI CCGG 1 cut(s) 203
MspR9I CCNGG 3 cut(s) 203, 257, 579
MvaI CCWGG 2 cut(s) 257, 579
MvnI CGCG 1 cut(s) 699
NarI GGCGCC 1 cut(s) 119
NciI CCSGG 1 cut(s) 203
NdeII GATC 2 cut(s) 229, 613
NlaIII CATG 6 cut(s) 290, 376, 412, 532, 776, 878
NlaIV GGNNCC 4 cut(s) 120, 200, 356, 357
NmuCI GTSAC 2 cut(s) 266, 815
NspI RCATGY 1 cut(s) 290
PceI AGGCCT 1 cut(s) 161
PciI ACATGT 1 cut(s) 286
PciSI GCTCTTC 1 cut(s) 310
PcsI WCGNNNNNNNCGW 1 cut(s) 804
PfeI GAWTC 4 cut(s) 340, 671, 761, 800
PflMI CCANNNNNTGG 1 cut(s) 262
PkrI GCNGC 2 cut(s) 35, 369
PluTI GGCGCC 1 cut(s) 122
PpuMI RGGWCCY 1 cut(s) 355
PscI ACATGT 1 cut(s) 286
Psp5II RGGWCCY 1 cut(s) 355
Psp6I CCWGG 2 cut(s) 255, 577
PspFI CCCAGC 1 cut(s) 505
PspGI CCWGG 2 cut(s) 255, 577
PspN4I GGNNCC 4 cut(s) 120, 200, 356, 357
PspPI GGNCC 3 cut(s) 205, 355, 581
PspPPI RGGWCCY 1 cut(s) 355
PstNI CAGNNNCTG 1 cut(s) 262
PsuI RGATCY 1 cut(s) 613
RsaI GTAC 3 cut(s) 654, 726, 927
RsaNI GTAC 3 cut(s) 653, 725, 926
RseI CAYNNNNRTG 1 cut(s) 789
SacII CCGCGG 1 cut(s) 700
SapI GCTCTTC 1 cut(s) 310
SaqAI TTAA 6 cut(s) 113, 180, 234, 498, 555, 624
SatI GCNGC 2 cut(s) 34, 368
Sau3AI GATC 2 cut(s) 229, 613
Sau96I GGNCC 3 cut(s) 205, 355, 581
ScrFI CCNGG 3 cut(s) 203, 257, 579
SduI GDGCHC 2 cut(s) 203, 611
SfaNI GCATC 2 cut(s) 579, 643
SfoI GGCGCC 1 cut(s) 120
Sfr303I CCGCGG 1 cut(s) 700
SgrBI CCGCGG 1 cut(s) 700
SinI GGWCC 3 cut(s) 205, 355, 581
SmiMI CAYNNNNRTG 1 cut(s) 789
SmlI CTYRAG 1 cut(s) 740
SmoI CTYRAG 1 cut(s) 740
Sse9I AATT 7 cut(s) 276, 442, 685, 755, 870, 881, 897
SseBI AGGCCT 1 cut(s) 161
SsiI CCGC 4 cut(s) 51, 368, 697, 699
SspDI GGCGCC 1 cut(s) 118
StuI AGGCCT 1 cut(s) 161
StyD4I CCNGG 3 cut(s) 201, 255, 577
StyI CCWWGG 1 cut(s) 216
TaaI ACNGT 3 cut(s) 41, 717, 821
TaiI ACGT 4 cut(s) 25, 273, 484, 735
TaqI TCGA 1 cut(s) 798
TasI AATT 7 cut(s) 276, 442, 685, 755, 870, 881, 897
TatI WGTACW 3 cut(s) 652, 724, 925
TauI GCSGC 1 cut(s) 370
TfiI GAWTC 4 cut(s) 340, 671, 761, 800
Tru1I TTAA 6 cut(s) 113, 180, 234, 498, 555, 624
Tru9I TTAA 6 cut(s) 113, 180, 234, 498, 555, 624
TscAI CASTG 3 cut(s) 35, 591, 826
TseFI GTSAC 2 cut(s) 266, 815
TseI GCWGC 1 cut(s) 33
Tsp45I GTSAC 2 cut(s) 266, 815
TspDTI ATGAA 4 cut(s) 517, 540, 807, 863
TspGWI ACGGA 2 cut(s) 606, 875
TspRI CASTG 3 cut(s) 35, 591, 826
Van91I CCANNNNNTGG 1 cut(s) 262
VpaK11BI GGWCC 3 cut(s) 205, 355, 581
XapI RAATTY 3 cut(s) 870, 881, 897
XceI RCATGY 1 cut(s) 290
XmiI GTMKAC 2 cut(s) 569, 912
ZraI GACGTC 1 cut(s) 733
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.