MD02G1232000.v1.1

tRNA (guanine-N(7)-)-methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0006250
Physical Location & Seq
Forward (+)
27784213 .. 27788300
4088 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1232000.v1.1.491

Sequence Viewer

Length: 948 bp
ATGCAGAGCAGCAAAATGCTGGGTTCTTGCGTAGGCGGTGGTACACTCAGTGCTGCATTACCCAGAAGAGCTCTGAAACTGAAACGGCTTATTTCTCCAAATTCACCACTATTACCTTCTCACCAATTTCACCCCATCAGCAGAAGATTGACCGCCTCTGCTTCTGCTGGTGCACAGCAGCAGCAACAGCAACAGCAACACCAGCAGCAGAGCCCCAACCTAGTGGCCTTGGAATATGCCGACCTTAATCTCAACCCCACAATCTCTGAGGAACTGGGTCTGGGTCATGGCCGAATCAGGCAACACGTCAACCCTCTGAAAACCTCTCTCTCTGCACCTGTGGAAGTACCTGATTGGAATCAAGTCTTCAGGGATCCAACATTGCCACTCATGGTGGATATTGGAAGTGGTAGCGGCAGATTTCTCTTATGGCTCGCAAAAAGGAATTTAGTTGTGAGAAATTATCTGGGACTGGAAATACGGAAGAAACTGGTCAAGCGTTCTCAATTCTGGGTTCAAGACCTGGCTCTTAGTAATATACATTTCATGTTTGCAAATGCCACAACTTCCTATCAACAACTAGTGTCTTCATACCCCGGACCATTGATGCTTGTTTCCATCCTGTGCCCAGATCCTTATTTCAAGAAAAGGCATCATAAGAGACGGGTTGTACAGAAGCCTTTAGTAGATTCCATTGTGAACAGTTTAATGCCAAGTGGCCAGGTGTTTATCCAATCCGACGTACTTGATGTGGCACTTGACATGAGAGATCAGTTTGATTCTCATTCCAATATCCTGACACACATCCACGAAATTGACACAAGTGTGCTGTGTGACAGCGAGGGATGGTTGTTAAACAATCCAATGGGAATTCGGACCGAGAGAGAAATTCATGCAGAGTTTGAAGGTGCAAAGATTTACAGGCGGGTGTACCAGAAGCAAACGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_4

Protein Analysis

316

Amino Acids

35.55

Weight (kDa)

9.65

Isoelectric Point (pI)

61.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_4 PF02390 129 - 307 6.4e-31 Putative methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 4 cut(s) 36, 153, 414, 925
AclWI GGATC 3 cut(s) 368, 381, 626
AcoI YGGCCR 2 cut(s) 289, 718
AcsI RAATTY 4 cut(s) 100, 445, 870, 888
AcuI CTGAAG 1 cut(s) 352
AdeI CACNNNGTG 1 cut(s) 50
AfaI GTAC 5 cut(s) 43, 348, 672, 744, 932
AflIII ACRYGT 1 cut(s) 304
AgsI TTSAA 3 cut(s) 518, 643, 905
AhlI ACTAGT 1 cut(s) 580
AjiI CACGTC 1 cut(s) 307
AjnI CCWGG 2 cut(s) 522, 720
AleI CACNNNNGTG 1 cut(s) 824
AluBI AGCT 1 cut(s) 71
AluI AGCT 1 cut(s) 71
Alw21I GWGCWC 2 cut(s) 73, 175
Alw26I GTCTC 1 cut(s) 655
Alw44I GTGCAC 1 cut(s) 171
AlwI GGATC 3 cut(s) 368, 381, 626
AoxI GGCC 3 cut(s) 225, 289, 718
ApaLI GTGCAC 1 cut(s) 171
ApeKI GCWGC 5 cut(s) 9, 53, 178, 181, 205
ApoI RAATTY 4 cut(s) 100, 445, 870, 888
AspS9I GGNCC 2 cut(s) 599, 876
AsuC2I CCSGG 1 cut(s) 597
AsuHPI GGTGA 3 cut(s) 96, 113, 122
AvaII GGWCC 2 cut(s) 599, 876
BaeGI GKGCMC 2 cut(s) 175, 629
BalI TGGCCA 1 cut(s) 720
BamHI GGATCC 1 cut(s) 373
BanII GRGCYC 2 cut(s) 73, 215
BbsI GAAGAC 2 cut(s) 358, 579
Bbv12I GWGCWC 2 cut(s) 73, 175
BbvI GCAGC 5 cut(s) 21, 40, 190, 193, 217
BccI CCATC 3 cut(s) 143, 626, 840
BceAI ACGGC 1 cut(s) 101
BciT130I CCWGG 2 cut(s) 524, 722
BcnI CCSGG 1 cut(s) 597
BcoDI GTCTC 1 cut(s) 655
BcuI ACTAGT 1 cut(s) 580
BfaI CTAG 2 cut(s) 221, 581
BisI GCNGC 6 cut(s) 10, 54, 179, 182, 206, 415
BlsI GCNGC 6 cut(s) 11, 55, 180, 183, 207, 416
Bme1390I CCNGG 3 cut(s) 524, 597, 722
Bme18I GGWCC 2 cut(s) 599, 876
BmgBI CACGTC 1 cut(s) 307
BmgT120I GGNCC 2 cut(s) 599, 876
BmiI GGNNCC 1 cut(s) 375
BmrFI CCNGG 3 cut(s) 524, 597, 722
BmrI ACTGGG 1 cut(s) 284
BmsI GCATC 2 cut(s) 597, 661
BmuI ACTGGG 1 cut(s) 284
BpiI GAAGAC 2 cut(s) 358, 579
BpuMI CCSGG 1 cut(s) 597
BsaBI GATNNNNATC 1 cut(s) 357
BsaJI CCNNGG 2 cut(s) 228, 595
Bse1I ACTGG 3 cut(s) 279, 477, 495
Bse3DI GCAATG 1 cut(s) 380
Bse8I GATNNNNATC 1 cut(s) 357
BseBI CCWGG 2 cut(s) 524, 722
BseDI CCNNGG 2 cut(s) 228, 595
BseGI GGATG 3 cut(s) 618, 804, 851
BseJI GATNNNNATC 1 cut(s) 357
BseMI GCAATG 1 cut(s) 380
BseMII CTCAG 2 cut(s) 61, 258
BseNI ACTGG 3 cut(s) 279, 477, 495
BseSI GKGCMC 2 cut(s) 175, 629
BseXI GCAGC 5 cut(s) 21, 40, 190, 193, 217
BseYI CCCAGC 1 cut(s) 19
BsgI GTGCAG 1 cut(s) 318
BshFI GGCC 3 cut(s) 227, 291, 720
BsiHKAI GWGCWC 2 cut(s) 73, 175
BsiSI CCGG 1 cut(s) 597
BslFI GGGAC 1 cut(s) 483
BsmAI GTCTC 1 cut(s) 655
BsmBI CGTCTC 1 cut(s) 655
BsmFI GGGAC 1 cut(s) 483
BsnI GGCC 3 cut(s) 227, 291, 720
Bsp1286I GDGCHC 4 cut(s) 73, 175, 215, 629
Bsp1407I TGTACA 1 cut(s) 670
Bsp143I GATC 3 cut(s) 373, 631, 769
BspACI CCGC 4 cut(s) 36, 153, 414, 925
BspANI GGCC 3 cut(s) 227, 291, 720
BspCNI CTCAG 2 cut(s) 60, 259
BspLI GGNNCC 1 cut(s) 375
BspPI GGATC 3 cut(s) 368, 381, 626
BspQI GCTCTTC 1 cut(s) 61
BsrDI GCAATG 1 cut(s) 380
BsrGI TGTACA 1 cut(s) 670
BsrI ACTGG 3 cut(s) 279, 477, 495
BssECI CCNNGG 2 cut(s) 228, 595
BssMI GATC 3 cut(s) 373, 631, 769
BssT1I CCWWGG 1 cut(s) 228
Bst2UI CCWGG 2 cut(s) 524, 722
Bst4CI ACNGT 1 cut(s) 704
Bst6I CTCTTC 1 cut(s) 61
BstAUI TGTACA 1 cut(s) 670
BstC8I GCNNGC 1 cut(s) 435
BstDEI CTNAG 3 cut(s) 47, 267, 530
BstF5I GGATG 3 cut(s) 618, 804, 851
BstKTI GATC 3 cut(s) 376, 634, 772
BstMAI GTCTC 1 cut(s) 655
BstMBI GATC 3 cut(s) 373, 631, 769
BstMWI GCNNNNNNNGC 2 cut(s) 187, 202
BstNI CCWGG 2 cut(s) 524, 722
BstSCI CCNGG 3 cut(s) 522, 595, 720
BstSLI GKGCMC 2 cut(s) 175, 629
BstV1I GCAGC 5 cut(s) 21, 40, 190, 193, 217
BstV2I GAAGAC 2 cut(s) 358, 579
BstX2I RGATCY 2 cut(s) 373, 631
BstXI CCANNNNNNTGG 1 cut(s) 223
BstYI RGATCY 2 cut(s) 373, 631
BsuRI GGCC 3 cut(s) 227, 291, 720
BtrI CACGTC 1 cut(s) 307
BtsCI GGATG 3 cut(s) 618, 804, 851
BtsIMutI CAGTG 1 cut(s) 55
Cac8I GCNNGC 1 cut(s) 435
Cfr13I GGNCC 2 cut(s) 599, 876
CpoI CGGWCCG 1 cut(s) 876
Csp6I GTAC 5 cut(s) 42, 347, 671, 743, 931
CspI CGGWCCG 1 cut(s) 876
CviAII CATG 5 cut(s) 287, 391, 547, 763, 893
CviJI RGCY 9 cut(s) 71, 88, 213, 227, 291, 433, 527, 679, 720
CviKI_1 RGCY 9 cut(s) 71, 88, 213, 227, 291, 433, 527, 679, 720
CviQI GTAC 5 cut(s) 42, 347, 671, 743, 931
DdeI CTNAG 3 cut(s) 47, 267, 530
DpnI GATC 3 cut(s) 375, 633, 771
DpnII GATC 3 cut(s) 373, 631, 769
DraIII CACNNNGTG 1 cut(s) 50
EaeI YGGCCR 2 cut(s) 289, 718
Eam1104I CTCTTC 1 cut(s) 61
EarI CTCTTC 1 cut(s) 61
Ecl136II GAGCTC 1 cut(s) 71
Eco130I CCWWGG 1 cut(s) 228
Eco24I GRGCYC 2 cut(s) 73, 215
Eco47I GGWCC 2 cut(s) 599, 876
Eco53kI GAGCTC 1 cut(s) 71
Eco57I CTGAAG 1 cut(s) 352
EcoICRI GAGCTC 1 cut(s) 71
EcoRI GAATTC 1 cut(s) 870
EcoRII CCWGG 2 cut(s) 522, 720
EcoT14I CCWWGG 1 cut(s) 228
EcoT38I GRGCYC 2 cut(s) 73, 215
ErhI CCWWGG 1 cut(s) 228
Esp3I CGTCTC 1 cut(s) 655
FaeI CATG 5 cut(s) 290, 394, 550, 766, 896
FaqI GGGAC 1 cut(s) 483
FatI CATG 5 cut(s) 286, 390, 546, 762, 892
FauI CCCGC 1 cut(s) 918
Fnu4HI GCNGC 6 cut(s) 10, 54, 179, 182, 206, 415
FokI GGATG 3 cut(s) 605, 791, 858
FriOI GRGCYC 2 cut(s) 73, 215
Fsp4HI GCNGC 6 cut(s) 10, 54, 179, 182, 206, 415
FspBI CTAG 2 cut(s) 221, 581
GluI GCNGC 6 cut(s) 10, 54, 179, 182, 206, 415
GsaI CCCAGC 1 cut(s) 23
HaeIII GGCC 3 cut(s) 227, 291, 720
HapII CCGG 1 cut(s) 597
Hin1II CATG 5 cut(s) 290, 394, 550, 766, 896
HincII GTYRAC 1 cut(s) 310
HindII GTYRAC 1 cut(s) 310
HinfI GANTC 4 cut(s) 294, 358, 689, 779
HpaII CCGG 1 cut(s) 597
HphI GGTGA 3 cut(s) 96, 113, 122
Hpy166II GTNNAC 5 cut(s) 44, 173, 310, 700, 931
Hpy188I TCNGA 5 cut(s) 75, 268, 318, 739, 876
Hpy188III TCNNGA 3 cut(s) 518, 643, 796
Hpy8I GTNNAC 5 cut(s) 44, 173, 310, 700, 931
Hpy99I CGWCG 1 cut(s) 743
HpyAV CCTTC 2 cut(s) 126, 899
HpyCH4III ACNGT 1 cut(s) 704
HpyCH4IV ACGT 3 cut(s) 306, 741, 944
HpyCH4V TGCA 7 cut(s) 4, 56, 173, 335, 554, 896, 911
HpyF10VI GCNNNNNNNGC 2 cut(s) 187, 202
HpyF3I CTNAG 3 cut(s) 47, 267, 530
HpySE526I ACGT 3 cut(s) 306, 741, 944
Hsp92II CATG 5 cut(s) 290, 394, 550, 766, 896
Kzo9I GATC 3 cut(s) 373, 631, 769
LguI GCTCTTC 1 cut(s) 61
Lsp1109I GCAGC 5 cut(s) 21, 40, 190, 193, 217
LweI GCATC 2 cut(s) 597, 661
MaeI CTAG 2 cut(s) 221, 581
MaeII ACGT 3 cut(s) 306, 741, 944
MaeIII GTNAC 1 cut(s) 833
MalI GATC 3 cut(s) 375, 633, 771
MboI GATC 3 cut(s) 373, 631, 769
MboII GAAGA 5 cut(s) 78, 156, 358, 496, 579
MflI RGATCY 2 cut(s) 373, 631
MhlI GDGCHC 4 cut(s) 73, 175, 215, 629
MlsI TGGCCA 1 cut(s) 720
MluCI AATT 8 cut(s) 100, 125, 445, 460, 506, 813, 870, 888
MluNI TGGCCA 1 cut(s) 720
MmeI TCCRAC 2 cut(s) 401, 762
MnlI CCTC 5 cut(s) 166, 262, 324, 334, 835
Mox20I TGGCCA 1 cut(s) 720
MscI TGGCCA 1 cut(s) 720
MseI TTAA 3 cut(s) 246, 707, 854
MslI CAYNNNNRTG 1 cut(s) 824
Msp20I TGGCCA 1 cut(s) 720
MspI CCGG 1 cut(s) 597
MspR9I CCNGG 3 cut(s) 524, 597, 722
MvaI CCWGG 2 cut(s) 524, 722
MwoI GCNNNNNNNGC 2 cut(s) 187, 202
NciI CCSGG 1 cut(s) 597
NdeII GATC 3 cut(s) 373, 631, 769
NlaIII CATG 5 cut(s) 290, 394, 550, 766, 896
NlaIV GGNNCC 1 cut(s) 375
NmuCI GTSAC 1 cut(s) 833
OliI CACNNNNGTG 1 cut(s) 824
PciSI GCTCTTC 1 cut(s) 61
PfeI GAWTC 4 cut(s) 294, 358, 689, 779
PkrI GCNGC 6 cut(s) 11, 55, 180, 183, 207, 416
Psp124BI GAGCTC 1 cut(s) 73
Psp6I CCWGG 2 cut(s) 522, 720
PspFI CCCAGC 1 cut(s) 19
PspGI CCWGG 2 cut(s) 522, 720
PspN4I GGNNCC 1 cut(s) 375
PspPI GGNCC 2 cut(s) 599, 876
PsuI RGATCY 2 cut(s) 373, 631
RsaI GTAC 5 cut(s) 43, 348, 672, 744, 932
RsaNI GTAC 5 cut(s) 42, 347, 671, 743, 931
RseI CAYNNNNRTG 1 cut(s) 824
Rsr2I CGGWCCG 1 cut(s) 876
RsrII CGGWCCG 1 cut(s) 876
SacI GAGCTC 1 cut(s) 73
SapI GCTCTTC 1 cut(s) 61
SaqAI TTAA 3 cut(s) 246, 707, 854
SatI GCNGC 6 cut(s) 10, 54, 179, 182, 206, 415
Sau3AI GATC 3 cut(s) 373, 631, 769
Sau96I GGNCC 2 cut(s) 599, 876
ScrFI CCNGG 3 cut(s) 524, 597, 722
SduI GDGCHC 4 cut(s) 73, 175, 215, 629
SfaNI GCATC 2 cut(s) 597, 661
SinI GGWCC 2 cut(s) 599, 876
SmiMI CAYNNNNRTG 1 cut(s) 824
SpeI ACTAGT 1 cut(s) 580
Sse9I AATT 8 cut(s) 100, 125, 445, 460, 506, 813, 870, 888
SsiI CCGC 4 cut(s) 36, 153, 414, 925
SspMI CTAG 2 cut(s) 221, 581
SstI GAGCTC 1 cut(s) 73
StyD4I CCNGG 3 cut(s) 522, 595, 720
StyI CCWWGG 1 cut(s) 228
TaaI ACNGT 1 cut(s) 704
TaiI ACGT 3 cut(s) 309, 744, 947
TaqII GACCGA 1 cut(s) 893
TasI AATT 8 cut(s) 100, 125, 445, 460, 506, 813, 870, 888
TatI WGTACW 1 cut(s) 670
TauI GCSGC 1 cut(s) 417
TfiI GAWTC 4 cut(s) 294, 358, 689, 779
Tru1I TTAA 3 cut(s) 246, 707, 854
Tru9I TTAA 3 cut(s) 246, 707, 854
TscAI CASTG 1 cut(s) 55
TseFI GTSAC 1 cut(s) 833
TseI GCWGC 5 cut(s) 9, 53, 178, 181, 205
Tsp45I GTSAC 1 cut(s) 833
TspDTI ATGAA 3 cut(s) 535, 579, 881
TspGWI ACGGA 1 cut(s) 496
TspRI CASTG 1 cut(s) 55
VneI GTGCAC 1 cut(s) 171
VpaK11BI GGWCC 2 cut(s) 599, 876
XapI RAATTY 4 cut(s) 100, 445, 870, 888
XspI CTAG 2 cut(s) 221, 581
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.