RchiOBHm_Chr1g0340671

tRNA (guanine-N(7)-)-methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0006250
Physical Location & Seq
Reverse (-)
32202153 .. 32206988
4836 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ56743

Sequence Viewer

Length: 261 bp
ATGCTTGTCAAACGTGCTGATGTCTGGGTTAAAGAGCTGGGTCTTAGTAACATACATTTCATGTATGCAAATGCCACTACTTCATTTAATCAACTGGTGTCTACCAACCCTGGACCACTGATGCTTGTTTCAATCCTGTGCCCAGATCCGTATTTTAAGAGAAAGCATCACAAGAGACGGGTTGTACAGAAGCCTTTAGTAGATTCCATTGTGAATAATTTAGCTCCCCGCGGACGTGTTTATACAGTCCGATGTACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_4

Protein Analysis

86

Amino Acids

9.85

Weight (kDa)

10.29

Isoelectric Point (pI)

38.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_4 PF02390 10 - 82 1.1e-15 Putative methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 101
AccII CGCG 1 cut(s) 231
AciI CCGC 2 cut(s) 229, 231
AclWI GGATC 1 cut(s) 140
AfaI GTAC 2 cut(s) 186, 256
AflIII ACRYGT 1 cut(s) 235
AgsI TTSAA 1 cut(s) 132
AjiI CACGTC 1 cut(s) 236
AjnI CCWGG 1 cut(s) 109
AluBI AGCT 2 cut(s) 37, 224
AluI AGCT 2 cut(s) 37, 224
Alw26I GTCTC 1 cut(s) 169
AlwI GGATC 1 cut(s) 140
AspS9I GGNCC 1 cut(s) 113
AvaII GGWCC 1 cut(s) 113
BaeGI GKGCMC 1 cut(s) 143
BciT130I CCWGG 1 cut(s) 111
BcoDI GTCTC 1 cut(s) 169
Bme1390I CCNGG 1 cut(s) 111
Bme18I GGWCC 1 cut(s) 113
BmgBI CACGTC 1 cut(s) 236
BmgT120I GGNCC 1 cut(s) 113
BmrFI CCNGG 1 cut(s) 111
BmsI GCATC 2 cut(s) 111, 175
BsaJI CCNNGG 2 cut(s) 109, 229
Bse1I ACTGG 1 cut(s) 99
BseBI CCWGG 1 cut(s) 111
BseDI CCNNGG 2 cut(s) 109, 229
BseNI ACTGG 1 cut(s) 99
BseSI GKGCMC 1 cut(s) 143
BseYI CCCAGC 1 cut(s) 37
Bsh1236I CGCG 1 cut(s) 231
BsmAI GTCTC 1 cut(s) 169
BsmBI CGTCTC 1 cut(s) 169
Bsp1286I GDGCHC 1 cut(s) 143
Bsp1407I TGTACA 1 cut(s) 184
Bsp143I GATC 1 cut(s) 145
BspACI CCGC 2 cut(s) 229, 231
BspFNI CGCG 1 cut(s) 231
BspPI GGATC 1 cut(s) 140
BsrGI TGTACA 1 cut(s) 184
BsrI ACTGG 1 cut(s) 99
BssECI CCNNGG 2 cut(s) 109, 229
BssMI GATC 1 cut(s) 145
Bst2UI CCWGG 1 cut(s) 111
Bst4CI ACNGT 1 cut(s) 247
BstAUI TGTACA 1 cut(s) 184
BstDEI CTNAG 1 cut(s) 44
BstDSI CCRYGG 1 cut(s) 229
BstFNI CGCG 1 cut(s) 231
BstKTI GATC 1 cut(s) 148
BstMAI GTCTC 1 cut(s) 169
BstMBI GATC 1 cut(s) 145
BstNI CCWGG 1 cut(s) 111
BstSCI CCNGG 1 cut(s) 109
BstSLI GKGCMC 1 cut(s) 143
BstUI CGCG 1 cut(s) 231
BstX2I RGATCY 1 cut(s) 145
BstYI RGATCY 1 cut(s) 145
BtgI CCRYGG 1 cut(s) 229
BtrI CACGTC 1 cut(s) 236
BtsIMutI CAGTG 1 cut(s) 116
Cfr13I GGNCC 1 cut(s) 113
Cfr42I CCGCGG 1 cut(s) 232
Csp6I GTAC 2 cut(s) 185, 255
CviAII CATG 1 cut(s) 61
CviJI RGCY 3 cut(s) 37, 193, 224
CviKI_1 RGCY 3 cut(s) 37, 193, 224
CviQI GTAC 2 cut(s) 185, 255
DdeI CTNAG 1 cut(s) 44
DpnI GATC 1 cut(s) 147
DpnII GATC 1 cut(s) 145
Eco47I GGWCC 1 cut(s) 113
EcoRII CCWGG 1 cut(s) 109
Esp3I CGTCTC 1 cut(s) 169
FaeI CATG 1 cut(s) 64
FaiI YATR 4 cut(s) 53, 62, 66, 243
FatI CATG 1 cut(s) 60
FauI CCCGC 1 cut(s) 236
FblI GTMKAC 1 cut(s) 101
GsaI CCCAGC 1 cut(s) 41
Hin1II CATG 1 cut(s) 64
HinfI GANTC 1 cut(s) 203
Hpy166II GTNNAC 1 cut(s) 102
Hpy188I TCNGA 1 cut(s) 251
Hpy8I GTNNAC 1 cut(s) 102
HpyCH4III ACNGT 1 cut(s) 247
HpyCH4IV ACGT 2 cut(s) 13, 235
HpyCH4V TGCA 1 cut(s) 68
HpyF3I CTNAG 1 cut(s) 44
HpySE526I ACGT 2 cut(s) 13, 235
Hsp92II CATG 1 cut(s) 64
KspI CCGCGG 1 cut(s) 232
Kzo9I GATC 1 cut(s) 145
LmnI GCTCC 1 cut(s) 229
LpnPI CCDG 7 cut(s) 10, 23, 80, 96, 123, 149, 156
LweI GCATC 2 cut(s) 111, 175
MaeII ACGT 2 cut(s) 13, 235
MaeIII GTNAC 1 cut(s) 47
MalI GATC 1 cut(s) 147
MboI GATC 1 cut(s) 145
MflI RGATCY 1 cut(s) 145
MhlI GDGCHC 1 cut(s) 143
MluCI AATT 1 cut(s) 217
MseI TTAA 3 cut(s) 30, 87, 156
MspA1I CMGCKG 1 cut(s) 231
MspR9I CCNGG 1 cut(s) 111
MvaI CCWGG 1 cut(s) 111
MvnI CGCG 1 cut(s) 231
NdeII GATC 1 cut(s) 145
NlaIII CATG 1 cut(s) 64
PfeI GAWTC 1 cut(s) 203
Psp6I CCWGG 1 cut(s) 109
PspFI CCCAGC 1 cut(s) 37
PspGI CCWGG 1 cut(s) 109
PspPI GGNCC 1 cut(s) 113
PsuI RGATCY 1 cut(s) 145
RsaI GTAC 2 cut(s) 186, 256
RsaNI GTAC 2 cut(s) 185, 255
SacII CCGCGG 1 cut(s) 232
SaqAI TTAA 3 cut(s) 30, 87, 156
Sau3AI GATC 1 cut(s) 145
Sau96I GGNCC 1 cut(s) 113
ScrFI CCNGG 1 cut(s) 111
SduI GDGCHC 1 cut(s) 143
SetI ASST 4 cut(s) 16, 39, 226, 238
SfaNI GCATC 2 cut(s) 111, 175
Sfr303I CCGCGG 1 cut(s) 232
SgrBI CCGCGG 1 cut(s) 232
SinI GGWCC 1 cut(s) 113
Sse9I AATT 1 cut(s) 217
SsiI CCGC 2 cut(s) 229, 231
StyD4I CCNGG 1 cut(s) 109
TaaI ACNGT 1 cut(s) 247
TaiI ACGT 2 cut(s) 16, 238
TasI AATT 1 cut(s) 217
TatI WGTACW 2 cut(s) 184, 254
TfiI GAWTC 1 cut(s) 203
Tru1I TTAA 3 cut(s) 30, 87, 156
Tru9I TTAA 3 cut(s) 30, 87, 156
TscAI CASTG 1 cut(s) 123
TspDTI ATGAA 2 cut(s) 49, 72
TspGWI ACGGA 1 cut(s) 138
TspRI CASTG 1 cut(s) 123
VpaK11BI GGWCC 1 cut(s) 113
XmiI GTMKAC 1 cut(s) 101
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.