Rh1DG178700

tRNA (guanine-N(7)-)-methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0006250
Physical Location & Seq
Forward (+)
36339307 .. 36350951
11645 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG178700.1

Sequence Viewer

Length: 666 bp
ATGGGTTCTTGCGTCTGCTTACGTCAGCACTGCTGCTACGGTGGTTTGGGCGGTGCATTTCAAAGAAGGGGTCTTTCTAGTCTGTTTCGAAAAGGGGTTGTTGCCCCAGGTTTAACGTCGCCGTTTTATCAGTATCAGAGGAACAGAAGAAGAGTGGAGGCCTGTGTTTCTACTGGGTTTAAGGAACAAGAGCTGAGGAGCCCGGACCTTGTGGCCTTGGAATATGCTGATCTTAATCTCACCCACAATGTTTCCCAGGAACTGGGTCACGTCAGAATTAGGCAACATGTGAACCCTCTTAGCTCTTCTTTATCTGTGCCTGTGGAAGTGCCTGATTGGAATCAAGTGTTCAGGGACCCAACATTGCCGCTCATGGTGGACATTGGAAGTGGCTTCAGTCATATATCTAATATTATGTCTGCGCAGGTGTTTATACAGTCCGATGTACTTGACGTCGCACTTGAGATGAGAAACCAATTTGATTCTCTGCCCCATGTTCTGAAGCACATAGATGAAATCGAATCCAGCGTGCTGTGTGACAGTGAGGGATGGTTGTTGAGCAATCCAATGGGAAAAAGGACGGAAAGAGAAATTCATGCTGAATTTGAAGAGGCAAAAATTTACAGGAGAGTGTACCAGAAGCAAGTACAGAAGGCTCTTGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_4

Protein Analysis

221

Amino Acids

24.99

Weight (kDa)

6.71

Isoelectric Point (pI)

68.12

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 415
AatII GACGTC 1 cut(s) 456
Acc16I TGCGCA 1 cut(s) 423
Acc36I ACCTGC 1 cut(s) 415
AccB7I CCANNNNNTGG 1 cut(s) 262
AccBSI CCGCTC 1 cut(s) 370
AciI CCGC 2 cut(s) 51, 368
AcsI RAATTY 3 cut(s) 591, 602, 618
AcuI CTGAAG 2 cut(s) 379, 521
AcyI GRCGYC 1 cut(s) 453
AfaI GTAC 3 cut(s) 447, 635, 648
AfiI CCNNNNNNNGG 1 cut(s) 262
AflIII ACRYGT 1 cut(s) 286
AgsI TTSAA 2 cut(s) 62, 608
AjiI CACGTC 1 cut(s) 271
AjnI CCWGG 2 cut(s) 106, 255
AluBI AGCT 2 cut(s) 193, 303
AluI AGCT 2 cut(s) 193, 303
AlwNI CAGNNNCTG 1 cut(s) 262
AoxI GGCC 2 cut(s) 159, 213
ApeKI GCWGC 1 cut(s) 33
ApoI RAATTY 3 cut(s) 591, 602, 618
ArsI GACNNNNNNTTYG 2 cut(s) 55, 87
AspLEI GCGC 1 cut(s) 424
AspS9I GGNCC 2 cut(s) 205, 355
AsuC2I CCSGG 1 cut(s) 203
AsuHPI GGTGA 1 cut(s) 232
AsuII TTCGAA 1 cut(s) 88
AvaII GGWCC 2 cut(s) 205, 355
BanII GRGCYC 1 cut(s) 203
BbvCI CCTCAGC 1 cut(s) 194
BbvI GCAGC 1 cut(s) 20
BccI CCATC 1 cut(s) 543
BceAI ACGGC 1 cut(s) 106
BciT130I CCWGG 2 cut(s) 108, 257
BcnI CCSGG 1 cut(s) 203
BfaI CTAG 1 cut(s) 78
BfuAI ACCTGC 1 cut(s) 415
BisI GCNGC 2 cut(s) 34, 368
BlsI GCNGC 2 cut(s) 35, 369
Bme1390I CCNGG 3 cut(s) 108, 203, 257
Bme18I GGWCC 2 cut(s) 205, 355
BmgBI CACGTC 1 cut(s) 271
BmgT120I GGNCC 2 cut(s) 205, 355
BmiI GGNNCC 3 cut(s) 200, 356, 357
BmrFI CCNGG 3 cut(s) 108, 203, 257
BmrI ACTGGG 2 cut(s) 183, 272
BmuI ACTGGG 2 cut(s) 183, 272
Bpu10I CCTNAGC 1 cut(s) 194
Bpu14I TTCGAA 1 cut(s) 88
BpuEI CTTGAG 1 cut(s) 482
BpuMI CCSGG 1 cut(s) 203
BsaBI GATNNNNATC 2 cut(s) 234, 339
BsaHI GRCGYC 1 cut(s) 453
BsaJI CCNNGG 3 cut(s) 106, 216, 255
Bsc4I CCNNNNNNNGG 1 cut(s) 262
Bse1I ACTGG 2 cut(s) 178, 267
Bse3DI GCAATG 1 cut(s) 362
Bse8I GATNNNNATC 2 cut(s) 234, 339
BseBI CCWGG 2 cut(s) 108, 257
BseDI CCNNGG 3 cut(s) 106, 216, 255
BseGI GGATG 1 cut(s) 554
BseJI GATNNNNATC 2 cut(s) 234, 339
BseLI CCNNNNNNNGG 1 cut(s) 262
BseMI GCAATG 1 cut(s) 362
BseMII CTCAG 1 cut(s) 185
BseNI ACTGG 2 cut(s) 178, 267
BseRI GAGGAG 1 cut(s) 211
BseXI GCAGC 1 cut(s) 20
BshFI GGCC 2 cut(s) 161, 215
BsiSI CCGG 1 cut(s) 203
BslFI GGGAC 1 cut(s) 368
BslI CCNNNNNNNGG 1 cut(s) 262
BsmFI GGGAC 1 cut(s) 368
BsnI GGCC 2 cut(s) 161, 215
Bsp119I TTCGAA 1 cut(s) 88
Bsp1286I GDGCHC 1 cut(s) 203
Bsp143I GATC 1 cut(s) 229
BspACI CCGC 2 cut(s) 51, 368
BspANI GGCC 2 cut(s) 161, 215
BspCNI CTCAG 1 cut(s) 186
BspLI GGNNCC 3 cut(s) 200, 356, 357
BspMI ACCTGC 1 cut(s) 415
BspQI GCTCTTC 1 cut(s) 310
BspT104I TTCGAA 1 cut(s) 88
BsrBI CCGCTC 1 cut(s) 370
BsrDI GCAATG 1 cut(s) 362
BsrI ACTGG 2 cut(s) 178, 267
BssECI CCNNGG 3 cut(s) 106, 216, 255
BssMI GATC 1 cut(s) 229
BssNI GRCGYC 1 cut(s) 453
BssT1I CCWWGG 1 cut(s) 216
Bst2UI CCWGG 2 cut(s) 108, 257
Bst4CI ACNGT 3 cut(s) 41, 438, 542
Bst6I CTCTTC 3 cut(s) 145, 310, 603
BstACI GRCGYC 1 cut(s) 453
BstBI TTCGAA 1 cut(s) 88
BstC8I GCNNGC 1 cut(s) 530
BstDEI CTNAG 2 cut(s) 194, 299
BstF5I GGATG 1 cut(s) 554
BstHHI GCGC 1 cut(s) 424
BstKTI GATC 1 cut(s) 232
BstMBI GATC 1 cut(s) 229
BstNI CCWGG 2 cut(s) 108, 257
BstNSI RCATGY 1 cut(s) 290
BstSCI CCNGG 3 cut(s) 106, 201, 255
BstV1I GCAGC 1 cut(s) 20
BsuRI GGCC 2 cut(s) 161, 215
BtrI CACGTC 1 cut(s) 271
BtsCI GGATG 1 cut(s) 554
BtsI GCAGTG 1 cut(s) 28
BtsIMutI CAGTG 2 cut(s) 28, 547
BveI ACCTGC 1 cut(s) 415
Cac8I GCNNGC 1 cut(s) 530
CaiI CAGNNNCTG 1 cut(s) 262
CfoI GCGC 1 cut(s) 424
Cfr13I GGNCC 2 cut(s) 205, 355
Csp6I GTAC 3 cut(s) 446, 634, 647
CviAII CATG 4 cut(s) 287, 373, 494, 596
CviJI RGCY 7 cut(s) 161, 193, 201, 215, 303, 393, 656
CviKI_1 RGCY 7 cut(s) 161, 193, 201, 215, 303, 393, 656
CviQI GTAC 3 cut(s) 446, 634, 647
DdeI CTNAG 2 cut(s) 194, 299
DpnI GATC 1 cut(s) 231
DpnII GATC 1 cut(s) 229
Eam1104I CTCTTC 3 cut(s) 145, 310, 603
EarI CTCTTC 3 cut(s) 145, 310, 603
Eco130I CCWWGG 1 cut(s) 216
Eco147I AGGCCT 1 cut(s) 161
Eco24I GRGCYC 1 cut(s) 203
Eco47I GGWCC 2 cut(s) 205, 355
Eco57I CTGAAG 2 cut(s) 379, 521
EcoO109I RGGNCCY 1 cut(s) 355
EcoRII CCWGG 2 cut(s) 106, 255
EcoT14I CCWWGG 1 cut(s) 216
EcoT38I GRGCYC 1 cut(s) 203
ErhI CCWWGG 1 cut(s) 216
FaeI CATG 4 cut(s) 290, 376, 497, 599
FaqI GGGAC 1 cut(s) 368
FatI CATG 4 cut(s) 286, 372, 493, 595
Fnu4HI GCNGC 2 cut(s) 34, 368
FokI GGATG 1 cut(s) 561
FriOI GRGCYC 1 cut(s) 203
Fsp4HI GCNGC 2 cut(s) 34, 368
FspBI CTAG 1 cut(s) 78
FspI TGCGCA 1 cut(s) 423
GlaI GCGC 1 cut(s) 423
GluI GCNGC 2 cut(s) 34, 368
HaeIII GGCC 2 cut(s) 161, 215
HapII CCGG 1 cut(s) 203
HhaI GCGC 1 cut(s) 424
Hin1I GRCGYC 1 cut(s) 453
Hin1II CATG 4 cut(s) 290, 376, 497, 599
Hin6I GCGC 1 cut(s) 422
HinP1I GCGC 1 cut(s) 422
HinfI GANTC 3 cut(s) 340, 482, 521
HpaII CCGG 1 cut(s) 203
HphI GGTGA 1 cut(s) 232
Hpy166II GTNNAC 3 cut(s) 292, 379, 634
Hpy188I TCNGA 4 cut(s) 138, 275, 442, 501
Hpy8I GTNNAC 3 cut(s) 292, 379, 634
Hpy99I CGWCG 2 cut(s) 121, 458
HpyAV CCTTC 2 cut(s) 60, 646
HpyCH4III ACNGT 3 cut(s) 41, 438, 542
HpyCH4IV ACGT 4 cut(s) 22, 116, 270, 453
HpyCH4V TGCA 1 cut(s) 56
HpyF3I CTNAG 2 cut(s) 194, 299
HpySE526I ACGT 4 cut(s) 22, 116, 270, 453
Hsp92I GRCGYC 1 cut(s) 453
Hsp92II CATG 4 cut(s) 290, 376, 497, 599
HspAI GCGC 1 cut(s) 422
KflI GGGWCCC 1 cut(s) 355
Kzo9I GATC 1 cut(s) 229
LguI GCTCTTC 1 cut(s) 310
LmnI GCTCC 1 cut(s) 198
Lsp1109I GCAGC 1 cut(s) 20
MaeI CTAG 1 cut(s) 78
MaeII ACGT 4 cut(s) 22, 116, 270, 453
MaeIII GTNAC 2 cut(s) 266, 536
MalI GATC 1 cut(s) 231
MbiI CCGCTC 1 cut(s) 370
MboI GATC 1 cut(s) 229
MboII GAAGA 4 cut(s) 159, 162, 297, 620
MhlI GDGCHC 1 cut(s) 203
MluCI AATT 5 cut(s) 276, 476, 591, 602, 618
MnlI CCTC 6 cut(s) 132, 151, 189, 306, 538, 604
MseI TTAA 3 cut(s) 113, 180, 234
MslI CAYNNNNRTG 1 cut(s) 510
MspI CCGG 1 cut(s) 203
MspR9I CCNGG 3 cut(s) 108, 203, 257
MvaI CCWGG 2 cut(s) 108, 257
NciI CCSGG 1 cut(s) 203
NdeII GATC 1 cut(s) 229
NlaIII CATG 4 cut(s) 290, 376, 497, 599
NlaIV GGNNCC 3 cut(s) 200, 356, 357
NmuCI GTSAC 2 cut(s) 266, 536
NsbI TGCGCA 1 cut(s) 423
NspI RCATGY 1 cut(s) 290
NspV TTCGAA 1 cut(s) 88
PaqCI CACCTGC 1 cut(s) 415
PceI AGGCCT 1 cut(s) 161
PciI ACATGT 1 cut(s) 286
PciSI GCTCTTC 1 cut(s) 310
PcsI WCGNNNNNNNCGW 1 cut(s) 525
PfeI GAWTC 3 cut(s) 340, 482, 521
PflMI CCANNNNNTGG 1 cut(s) 262
PkrI GCNGC 2 cut(s) 35, 369
PpuMI RGGWCCY 1 cut(s) 355
PscI ACATGT 1 cut(s) 286
Psp5II RGGWCCY 1 cut(s) 355
Psp6I CCWGG 2 cut(s) 106, 255
PspGI CCWGG 2 cut(s) 106, 255
PspN4I GGNNCC 3 cut(s) 200, 356, 357
PspPI GGNCC 2 cut(s) 205, 355
PspPPI RGGWCCY 1 cut(s) 355
PstNI CAGNNNCTG 1 cut(s) 262
RsaI GTAC 3 cut(s) 447, 635, 648
RsaNI GTAC 3 cut(s) 446, 634, 647
RseI CAYNNNNRTG 1 cut(s) 510
SapI GCTCTTC 1 cut(s) 310
SaqAI TTAA 3 cut(s) 113, 180, 234
SatI GCNGC 2 cut(s) 34, 368
Sau3AI GATC 1 cut(s) 229
Sau96I GGNCC 2 cut(s) 205, 355
ScrFI CCNGG 3 cut(s) 108, 203, 257
SduI GDGCHC 1 cut(s) 203
SetI ASST 9 cut(s) 25, 112, 119, 195, 210, 273, 305, 429, 456
SfuI TTCGAA 1 cut(s) 88
SinI GGWCC 2 cut(s) 205, 355
SmiMI CAYNNNNRTG 1 cut(s) 510
SmlI CTYRAG 1 cut(s) 461
SmoI CTYRAG 1 cut(s) 461
Sse9I AATT 5 cut(s) 276, 476, 591, 602, 618
SseBI AGGCCT 1 cut(s) 161
SsiI CCGC 2 cut(s) 51, 368
SspI AATATT 1 cut(s) 412
SspMI CTAG 1 cut(s) 78
StuI AGGCCT 1 cut(s) 161
StyD4I CCNGG 3 cut(s) 106, 201, 255
StyI CCWWGG 1 cut(s) 216
TaaI ACNGT 3 cut(s) 41, 438, 542
TaiI ACGT 4 cut(s) 25, 119, 273, 456
TaqI TCGA 2 cut(s) 88, 519
TasI AATT 5 cut(s) 276, 476, 591, 602, 618
TatI WGTACW 2 cut(s) 445, 646
TauI GCSGC 1 cut(s) 370
TfiI GAWTC 3 cut(s) 340, 482, 521
Tru1I TTAA 3 cut(s) 113, 180, 234
Tru9I TTAA 3 cut(s) 113, 180, 234
TscAI CASTG 2 cut(s) 35, 547
TseFI GTSAC 2 cut(s) 266, 536
TseI GCWGC 1 cut(s) 33
Tsp45I GTSAC 2 cut(s) 266, 536
TspDTI ATGAA 2 cut(s) 528, 584
TspGWI ACGGA 1 cut(s) 596
TspRI CASTG 2 cut(s) 35, 547
Van91I CCANNNNNTGG 1 cut(s) 262
VpaK11BI GGWCC 2 cut(s) 205, 355
XapI RAATTY 3 cut(s) 591, 602, 618
XceI RCATGY 1 cut(s) 290
XspI CTAG 1 cut(s) 78
ZraI GACGTC 1 cut(s) 454
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.