Rroxscaffold_4G00315920

tRNA (guanine-N(7)-)-methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0006250
Physical Location & Seq
Forward (+)
40510360 .. 40516471
6112 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00315920.1

Sequence Viewer

Length: 849 bp
ATGGGTTCTTGCGTCTGCTTACGTCAGCACTACTGCTACGGTGGTGTGGGCGGTGCATTTCAAAGAAGGGGTCTTTCTAGTCTGTTTAACGGCGCCGTTTTACTGGAACAGAAGAAGAGTGGAGGCCTGTGTTTCTACTGGGTCTCTGTTTTTGTTCTTATGGAACTGGGTCACGTCAGAATTAGGCAACATGTGAATCCTCTTAGCTCTTCTTTATCTGCGCCTGTGGAAGTGCCTGATTGGAATCATGTGTTCAGGGACCCAACATTGCCGCTCATGGTGGACATTGGAAGTGGTAGTGGTAGATTTCTCATGTGGCTGGCGAAAAGAAATTTAGTTGTGAGGAATTATTTGGGACTGGAAATAAGGCAGAAACTTGTCAAACGTGCTGATGTCTGGGTTAAAGAGCTGGGTCTTAGTAATATACATTTCATGTATGCAAATGCCACTACTTCATTTACTCAACTGGTGTCTACCTACCCTGGACCACTGATGCTTGTTTCAATCCTGTGCCCAGATCCGTATTTTAAGAGAAAGCATCACAAGAGACGGGTTGTACAGAAGCCTTTAGTAGATTCCATTGTGAATTATTTAGCTCCCCACGGACAGGTGTTTATACAGTCCGATGTACTTGACGTTGCACTTGAGATGAGAAACCAATTTGATTCTCTGCCCCATGTTCTGAAGCACATAGATGAAATCGAATCCAGCGTGTTGTGTGACAGTGAGGGATGGTTGTTGAGCAATCCAATGGGCAAAAGGACGGAAAGAGAAATTCATGCTGAATTTGAAGGGGCAAAAATTTACAGGAGAGTGTACCAGAAGCGAGTACAGAAGGCTCTTGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_4

Protein Analysis

282

Amino Acids

32.1

Weight (kDa)

9.45

Isoelectric Point (pI)

49.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_4 PF02390 91 - 228 3.3e-31 Putative methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 92
AccBSI CCGCTC 1 cut(s) 274
AccI GTMKAC 1 cut(s) 473
AciI CCGC 2 cut(s) 51, 272
AclWI GGATC 1 cut(s) 512
AcsI RAATTY 4 cut(s) 331, 774, 785, 801
AcuI CTGAAG 1 cut(s) 704
AcyI GRCGYC 1 cut(s) 93
AfaI GTAC 4 cut(s) 558, 630, 818, 831
AfiI CCNNNNNNNGG 1 cut(s) 607
AflIII ACRYGT 1 cut(s) 190
AgsI TTSAA 3 cut(s) 62, 504, 791
AjiI CACGTC 1 cut(s) 175
AjnI CCWGG 1 cut(s) 481
AluBI AGCT 3 cut(s) 207, 409, 596
AluI AGCT 3 cut(s) 207, 409, 596
Alw26I GTCTC 2 cut(s) 148, 541
AlwI GGATC 1 cut(s) 512
AoxI GGCC 1 cut(s) 124
ApoI RAATTY 4 cut(s) 331, 774, 785, 801
ArsI GACNNNNNNTTYG 2 cut(s) 55, 87
AspLEI GCGC 2 cut(s) 95, 223
AspS9I GGNCC 2 cut(s) 259, 485
AvaII GGWCC 2 cut(s) 259, 485
BaeGI GKGCMC 1 cut(s) 515
BanI GGYRCC 1 cut(s) 92
BccI CCATC 1 cut(s) 726
BceAI ACGGC 2 cut(s) 80, 106
BciT130I CCWGG 1 cut(s) 483
BcoDI GTCTC 2 cut(s) 148, 541
BfaI CTAG 1 cut(s) 78
BfoI RGCGCY 1 cut(s) 96
BisI GCNGC 1 cut(s) 272
BlsI GCNGC 1 cut(s) 273
Bme1390I CCNGG 1 cut(s) 483
Bme18I GGWCC 2 cut(s) 259, 485
BmgBI CACGTC 1 cut(s) 175
BmgT120I GGNCC 2 cut(s) 259, 485
BmiI GGNNCC 3 cut(s) 94, 260, 261
BmrFI CCNGG 1 cut(s) 483
BmrI ACTGGG 2 cut(s) 148, 176
BmsI GCATC 2 cut(s) 483, 547
BmuI ACTGGG 2 cut(s) 148, 176
BpuEI CTTGAG 1 cut(s) 665
BsaBI GATNNNNATC 1 cut(s) 243
BsaHI GRCGYC 1 cut(s) 93
BsaI GGTCTC 1 cut(s) 148
BsaJI CCNNGG 2 cut(s) 481, 601
Bsc4I CCNNNNNNNGG 1 cut(s) 607
Bse1I ACTGG 5 cut(s) 108, 143, 171, 363, 471
Bse3DI GCAATG 1 cut(s) 266
Bse8I GATNNNNATC 1 cut(s) 243
BseBI CCWGG 1 cut(s) 483
BseDI CCNNGG 2 cut(s) 481, 601
BseGI GGATG 1 cut(s) 737
BseJI GATNNNNATC 1 cut(s) 243
BseLI CCNNNNNNNGG 1 cut(s) 607
BseMI GCAATG 1 cut(s) 266
BseNI ACTGG 5 cut(s) 108, 143, 171, 363, 471
BseSI GKGCMC 1 cut(s) 515
BseYI CCCAGC 1 cut(s) 409
BshFI GGCC 1 cut(s) 126
BshNI GGYRCC 1 cut(s) 92
BslFI GGGAC 2 cut(s) 272, 369
BslI CCNNNNNNNGG 1 cut(s) 607
BsmAI GTCTC 2 cut(s) 148, 541
BsmBI CGTCTC 1 cut(s) 541
BsmFI GGGAC 2 cut(s) 272, 369
BsnI GGCC 1 cut(s) 126
Bso31I GGTCTC 1 cut(s) 148
Bsp1286I GDGCHC 1 cut(s) 515
Bsp1407I TGTACA 1 cut(s) 556
Bsp143I GATC 1 cut(s) 517
BspACI CCGC 2 cut(s) 51, 272
BspANI GGCC 1 cut(s) 126
BspLI GGNNCC 3 cut(s) 94, 260, 261
BspPI GGATC 1 cut(s) 512
BspQI GCTCTTC 1 cut(s) 214
BspT107I GGYRCC 1 cut(s) 92
BspTNI GGTCTC 1 cut(s) 148
BsrBI CCGCTC 1 cut(s) 274
BsrDI GCAATG 1 cut(s) 266
BsrGI TGTACA 1 cut(s) 556
BsrI ACTGG 5 cut(s) 108, 143, 171, 363, 471
BssECI CCNNGG 2 cut(s) 481, 601
BssMI GATC 1 cut(s) 517
BssNI GRCGYC 1 cut(s) 93
Bst2UI CCWGG 1 cut(s) 483
Bst4CI ACNGT 3 cut(s) 41, 621, 725
Bst6I CTCTTC 2 cut(s) 110, 214
BstACI GRCGYC 1 cut(s) 93
BstAUI TGTACA 1 cut(s) 556
BstC8I GCNNGC 1 cut(s) 321
BstDEI CTNAG 2 cut(s) 203, 416
BstDSI CCRYGG 1 cut(s) 601
BstF5I GGATG 1 cut(s) 737
BstH2I RGCGCY 1 cut(s) 96
BstHHI GCGC 2 cut(s) 95, 223
BstKTI GATC 1 cut(s) 520
BstMAI GTCTC 2 cut(s) 148, 541
BstMBI GATC 1 cut(s) 517
BstNI CCWGG 1 cut(s) 483
BstNSI RCATGY 1 cut(s) 194
BstSCI CCNGG 1 cut(s) 481
BstSLI GKGCMC 1 cut(s) 515
BstX2I RGATCY 1 cut(s) 517
BstYI RGATCY 1 cut(s) 517
BsuRI GGCC 1 cut(s) 126
BtgI CCRYGG 1 cut(s) 601
BtrI CACGTC 1 cut(s) 175
BtsCI GGATG 1 cut(s) 737
BtsIMutI CAGTG 2 cut(s) 488, 730
Cac8I GCNNGC 1 cut(s) 321
CfoI GCGC 2 cut(s) 95, 223
Cfr13I GGNCC 2 cut(s) 259, 485
Csp6I GTAC 4 cut(s) 557, 629, 817, 830
CviAII CATG 7 cut(s) 191, 248, 277, 313, 433, 677, 779
CviJI RGCY 7 cut(s) 126, 207, 319, 409, 565, 596, 839
CviKI_1 RGCY 7 cut(s) 126, 207, 319, 409, 565, 596, 839
CviQI GTAC 4 cut(s) 557, 629, 817, 830
DdeI CTNAG 2 cut(s) 203, 416
DinI GGCGCC 1 cut(s) 94
DpnI GATC 1 cut(s) 519
DpnII GATC 1 cut(s) 517
Eam1104I CTCTTC 2 cut(s) 110, 214
EarI CTCTTC 2 cut(s) 110, 214
Eco147I AGGCCT 1 cut(s) 126
Eco31I GGTCTC 1 cut(s) 148
Eco47I GGWCC 2 cut(s) 259, 485
Eco57I CTGAAG 1 cut(s) 704
EcoO109I RGGNCCY 1 cut(s) 259
EcoRII CCWGG 1 cut(s) 481
EgeI GGCGCC 1 cut(s) 94
EheI GGCGCC 1 cut(s) 94
Esp3I CGTCTC 1 cut(s) 541
FaeI CATG 7 cut(s) 194, 251, 280, 316, 436, 680, 782
FaqI GGGAC 2 cut(s) 272, 369
FatI CATG 7 cut(s) 190, 247, 276, 312, 432, 676, 778
FblI GTMKAC 1 cut(s) 473
Fnu4HI GCNGC 1 cut(s) 272
FokI GGATG 1 cut(s) 744
Fsp4HI GCNGC 1 cut(s) 272
FspBI CTAG 1 cut(s) 78
GlaI GCGC 2 cut(s) 94, 222
GluI GCNGC 1 cut(s) 272
GsaI CCCAGC 1 cut(s) 413
HaeII RGCGCY 1 cut(s) 96
HaeIII GGCC 1 cut(s) 126
HhaI GCGC 2 cut(s) 95, 223
Hin1I GRCGYC 1 cut(s) 93
Hin1II CATG 7 cut(s) 194, 251, 280, 316, 436, 680, 782
Hin6I GCGC 2 cut(s) 93, 221
HinP1I GCGC 2 cut(s) 93, 221
HinfI GANTC 5 cut(s) 196, 244, 575, 665, 704
Hpy166II GTNNAC 3 cut(s) 283, 474, 817
Hpy188I TCNGA 3 cut(s) 179, 625, 684
Hpy8I GTNNAC 3 cut(s) 283, 474, 817
HpyAV CCTTC 3 cut(s) 60, 785, 829
HpyCH4III ACNGT 3 cut(s) 41, 621, 725
HpyCH4IV ACGT 4 cut(s) 22, 174, 385, 636
HpyCH4V TGCA 3 cut(s) 56, 440, 641
HpyF3I CTNAG 2 cut(s) 203, 416
HpySE526I ACGT 4 cut(s) 22, 174, 385, 636
Hsp92I GRCGYC 1 cut(s) 93
Hsp92II CATG 7 cut(s) 194, 251, 280, 316, 436, 680, 782
HspAI GCGC 2 cut(s) 93, 221
KasI GGCGCC 1 cut(s) 92
KflI GGGWCCC 1 cut(s) 259
Kzo9I GATC 1 cut(s) 517
LguI GCTCTTC 1 cut(s) 214
LmnI GCTCC 1 cut(s) 601
LweI GCATC 2 cut(s) 483, 547
MaeI CTAG 1 cut(s) 78
MaeII ACGT 4 cut(s) 22, 174, 385, 636
MaeIII GTNAC 2 cut(s) 170, 719
MalI GATC 1 cut(s) 519
MbiI CCGCTC 1 cut(s) 274
MboI GATC 1 cut(s) 517
MboII GAAGA 3 cut(s) 124, 127, 201
MflI RGATCY 1 cut(s) 517
MhlI GDGCHC 1 cut(s) 515
MluCI AATT 8 cut(s) 180, 331, 346, 586, 659, 774, 785, 801
Mly113I GGCGCC 1 cut(s) 93
MnlI CCTC 4 cut(s) 116, 210, 336, 721
MseI TTAA 3 cut(s) 87, 402, 528
MslI CAYNNNNRTG 1 cut(s) 693
MspR9I CCNGG 1 cut(s) 483
MvaI CCWGG 1 cut(s) 483
NarI GGCGCC 1 cut(s) 93
NdeII GATC 1 cut(s) 517
NlaIII CATG 7 cut(s) 194, 251, 280, 316, 436, 680, 782
NlaIV GGNNCC 3 cut(s) 94, 260, 261
NmuCI GTSAC 2 cut(s) 170, 719
NspI RCATGY 1 cut(s) 194
PceI AGGCCT 1 cut(s) 126
PciI ACATGT 1 cut(s) 190
PciSI GCTCTTC 1 cut(s) 214
PcsI WCGNNNNNNNCGW 1 cut(s) 708
PfeI GAWTC 5 cut(s) 196, 244, 575, 665, 704
PkrI GCNGC 1 cut(s) 273
PluTI GGCGCC 1 cut(s) 96
PpuMI RGGWCCY 1 cut(s) 259
PscI ACATGT 1 cut(s) 190
Psp5II RGGWCCY 1 cut(s) 259
Psp6I CCWGG 1 cut(s) 481
PspFI CCCAGC 1 cut(s) 409
PspGI CCWGG 1 cut(s) 481
PspN4I GGNNCC 3 cut(s) 94, 260, 261
PspPI GGNCC 2 cut(s) 259, 485
PspPPI RGGWCCY 1 cut(s) 259
PsuI RGATCY 1 cut(s) 517
RsaI GTAC 4 cut(s) 558, 630, 818, 831
RsaNI GTAC 4 cut(s) 557, 629, 817, 830
RseI CAYNNNNRTG 1 cut(s) 693
SapI GCTCTTC 1 cut(s) 214
SaqAI TTAA 3 cut(s) 87, 402, 528
SatI GCNGC 1 cut(s) 272
Sau3AI GATC 1 cut(s) 517
Sau96I GGNCC 2 cut(s) 259, 485
ScrFI CCNGG 1 cut(s) 483
SduI GDGCHC 1 cut(s) 515
SetI ASST 9 cut(s) 25, 177, 209, 388, 411, 479, 598, 612, 639
SfaNI GCATC 2 cut(s) 483, 547
SfoI GGCGCC 1 cut(s) 94
SinI GGWCC 2 cut(s) 259, 485
SmiMI CAYNNNNRTG 1 cut(s) 693
SmlI CTYRAG 1 cut(s) 644
SmoI CTYRAG 1 cut(s) 644
Sse9I AATT 8 cut(s) 180, 331, 346, 586, 659, 774, 785, 801
SseBI AGGCCT 1 cut(s) 126
SsiI CCGC 2 cut(s) 51, 272
SspDI GGCGCC 1 cut(s) 92
SspMI CTAG 1 cut(s) 78
StuI AGGCCT 1 cut(s) 126
StyD4I CCNGG 1 cut(s) 481
TaaI ACNGT 3 cut(s) 41, 621, 725
TaiI ACGT 4 cut(s) 25, 177, 388, 639
TaqI TCGA 1 cut(s) 702
TasI AATT 8 cut(s) 180, 331, 346, 586, 659, 774, 785, 801
TatI WGTACW 3 cut(s) 556, 628, 829
TauI GCSGC 1 cut(s) 274
TfiI GAWTC 5 cut(s) 196, 244, 575, 665, 704
Tru1I TTAA 3 cut(s) 87, 402, 528
Tru9I TTAA 3 cut(s) 87, 402, 528
TscAI CASTG 2 cut(s) 495, 730
TseFI GTSAC 2 cut(s) 170, 719
Tsp45I GTSAC 2 cut(s) 170, 719
TspDTI ATGAA 4 cut(s) 421, 444, 711, 767
TspGWI ACGGA 3 cut(s) 510, 618, 779
TspRI CASTG 2 cut(s) 495, 730
VpaK11BI GGWCC 2 cut(s) 259, 485
XapI RAATTY 4 cut(s) 331, 774, 785, 801
XceI RCATGY 1 cut(s) 194
XmiI GTMKAC 1 cut(s) 473
XspI CTAG 1 cut(s) 78
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.