Rh1AG177700

tRNA (guanine-N(7)-)-methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0006250
Physical Location & Seq
Forward (+)
33880210 .. 33886266
6057 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG177700.1

Sequence Viewer

Length: 504 bp
ATGTACTGTTCTTTGTTTTTCCTGATGCAGCTTGTCAAACGTGCTGATGTCTGGGTTAAAGAGCTGGGTCTTAGTAACATACATTTCATGTATGCAAATGCCACTACTTCATTTAATCAACTGGTGTCTACCTACCCTGGACCACTGATGCTTGTTTCAATCCTGTGCCCAGATCCGTATTTTAAGAGAAAGCATCACAAGAGACGGGTTGTACAGAAGCCTTTAGTAGATTCCATTGTGAATAATTTAGCTCCCCGCGGACAGGTGTTTATACAGTCCGATGTACTTGACGTCGCACTTGAGATGAGAAACCAATTTGATTCTCTGCCCCATGTTCTGAAGCACATAGATGAAATCGAATCCAGCGTGTTGTGTGACAGTGAGGGATGGTTGTTGAGCAATCCAACGGGAAAAAGGACGGAAAGAGAAATTCATGCTGAATTTGAAGGGGCAAAAATTTACAGGAGAGTATACCAGAAGCAAGTACAGAAGGCTCTTGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_4

Protein Analysis

167

Amino Acids

19.28

Weight (kDa)

8.99

Isoelectric Point (pI)

50.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_4 PF02390 12 - 113 2.5e-20 Putative methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 294
AccI GTMKAC 2 cut(s) 128, 471
AccII CGCG 1 cut(s) 258
AciI CCGC 2 cut(s) 256, 258
AclWI GGATC 1 cut(s) 167
AcsI RAATTY 3 cut(s) 429, 440, 456
AcuI CTGAAG 1 cut(s) 359
AcyI GRCGYC 1 cut(s) 291
AfaI GTAC 4 cut(s) 5, 213, 285, 486
AfiI CCNNNNNNNGG 1 cut(s) 262
AgsI TTSAA 2 cut(s) 159, 446
AjnI CCWGG 1 cut(s) 136
AluBI AGCT 3 cut(s) 31, 64, 251
AluI AGCT 3 cut(s) 31, 64, 251
Alw26I GTCTC 1 cut(s) 196
AlwI GGATC 1 cut(s) 167
ApeKI GCWGC 1 cut(s) 28
ApoI RAATTY 3 cut(s) 429, 440, 456
AspS9I GGNCC 1 cut(s) 140
AvaII GGWCC 1 cut(s) 140
BaeGI GKGCMC 1 cut(s) 170
BbvI GCAGC 1 cut(s) 40
BccI CCATC 1 cut(s) 381
BciT130I CCWGG 1 cut(s) 138
BcoDI GTCTC 1 cut(s) 196
BisI GCNGC 1 cut(s) 29
BlsI GCNGC 1 cut(s) 30
Bme1390I CCNGG 1 cut(s) 138
Bme18I GGWCC 1 cut(s) 140
BmgT120I GGNCC 1 cut(s) 140
BmrFI CCNGG 1 cut(s) 138
BmsI GCATC 3 cut(s) 15, 138, 202
BpuEI CTTGAG 1 cut(s) 320
BsaHI GRCGYC 1 cut(s) 291
BsaJI CCNNGG 2 cut(s) 136, 256
Bsc4I CCNNNNNNNGG 1 cut(s) 262
Bse1I ACTGG 1 cut(s) 126
BseBI CCWGG 1 cut(s) 138
BseDI CCNNGG 2 cut(s) 136, 256
BseGI GGATG 1 cut(s) 392
BseLI CCNNNNNNNGG 1 cut(s) 262
BseNI ACTGG 1 cut(s) 126
BseSI GKGCMC 1 cut(s) 170
BseXI GCAGC 1 cut(s) 40
BseYI CCCAGC 1 cut(s) 64
Bsh1236I CGCG 1 cut(s) 258
BslI CCNNNNNNNGG 1 cut(s) 262
BsmAI GTCTC 1 cut(s) 196
BsmBI CGTCTC 1 cut(s) 196
Bsp1286I GDGCHC 1 cut(s) 170
Bsp1407I TGTACA 1 cut(s) 211
Bsp143I GATC 1 cut(s) 172
BspACI CCGC 2 cut(s) 256, 258
BspFNI CGCG 1 cut(s) 258
BspPI GGATC 1 cut(s) 167
BsrGI TGTACA 1 cut(s) 211
BsrI ACTGG 1 cut(s) 126
BssECI CCNNGG 2 cut(s) 136, 256
BssMI GATC 1 cut(s) 172
BssNAI GTATAC 1 cut(s) 472
BssNI GRCGYC 1 cut(s) 291
Bst1107I GTATAC 1 cut(s) 472
Bst2UI CCWGG 1 cut(s) 138
Bst4CI ACNGT 3 cut(s) 8, 276, 380
BstACI GRCGYC 1 cut(s) 291
BstAUI TGTACA 1 cut(s) 211
BstDEI CTNAG 1 cut(s) 71
BstDSI CCRYGG 1 cut(s) 256
BstF5I GGATG 1 cut(s) 392
BstFNI CGCG 1 cut(s) 258
BstKTI GATC 1 cut(s) 175
BstMAI GTCTC 1 cut(s) 196
BstMBI GATC 1 cut(s) 172
BstNI CCWGG 1 cut(s) 138
BstSCI CCNGG 1 cut(s) 136
BstSLI GKGCMC 1 cut(s) 170
BstUI CGCG 1 cut(s) 258
BstV1I GCAGC 1 cut(s) 40
BstX2I RGATCY 1 cut(s) 172
BstYI RGATCY 1 cut(s) 172
BstZ17I GTATAC 1 cut(s) 472
BtgI CCRYGG 1 cut(s) 256
BtsCI GGATG 1 cut(s) 392
BtsIMutI CAGTG 2 cut(s) 143, 385
Cfr13I GGNCC 1 cut(s) 140
Cfr42I CCGCGG 1 cut(s) 259
Csp6I GTAC 4 cut(s) 4, 212, 284, 485
CviAII CATG 3 cut(s) 88, 332, 434
CviJI RGCY 5 cut(s) 31, 64, 220, 251, 494
CviKI_1 RGCY 5 cut(s) 31, 64, 220, 251, 494
CviQI GTAC 4 cut(s) 4, 212, 284, 485
DdeI CTNAG 1 cut(s) 71
DpnI GATC 1 cut(s) 174
DpnII GATC 1 cut(s) 172
Eco47I GGWCC 1 cut(s) 140
Eco57I CTGAAG 1 cut(s) 359
EcoRII CCWGG 1 cut(s) 136
Esp3I CGTCTC 1 cut(s) 196
FaeI CATG 3 cut(s) 91, 335, 437
FaiI YATR 8 cut(s) 80, 89, 93, 272, 333, 347, 435, 472
FatI CATG 3 cut(s) 87, 331, 433
FauI CCCGC 1 cut(s) 263
FblI GTMKAC 2 cut(s) 128, 471
Fnu4HI GCNGC 1 cut(s) 29
FokI GGATG 1 cut(s) 399
Fsp4HI GCNGC 1 cut(s) 29
GluI GCNGC 1 cut(s) 29
GsaI CCCAGC 1 cut(s) 68
Hin1I GRCGYC 1 cut(s) 291
Hin1II CATG 3 cut(s) 91, 335, 437
HinfI GANTC 3 cut(s) 230, 320, 359
Hpy166II GTNNAC 2 cut(s) 129, 472
Hpy188I TCNGA 2 cut(s) 280, 339
Hpy188III TCNNGA 1 cut(s) 22
Hpy8I GTNNAC 2 cut(s) 129, 472
Hpy99I CGWCG 1 cut(s) 296
HpyAV CCTTC 2 cut(s) 440, 484
HpyCH4III ACNGT 3 cut(s) 8, 276, 380
HpyCH4IV ACGT 2 cut(s) 40, 291
HpyCH4V TGCA 2 cut(s) 28, 95
HpyF3I CTNAG 1 cut(s) 71
HpySE526I ACGT 2 cut(s) 40, 291
Hsp92I GRCGYC 1 cut(s) 291
Hsp92II CATG 3 cut(s) 91, 335, 437
KspI CCGCGG 1 cut(s) 259
Kzo9I GATC 1 cut(s) 172
LmnI GCTCC 1 cut(s) 256
Lsp1109I GCAGC 1 cut(s) 40
LweI GCATC 3 cut(s) 15, 138, 202
MaeII ACGT 2 cut(s) 40, 291
MaeIII GTNAC 2 cut(s) 74, 374
MalI GATC 1 cut(s) 174
MboI GATC 1 cut(s) 172
MflI RGATCY 1 cut(s) 172
MhlI GDGCHC 1 cut(s) 170
MluCI AATT 5 cut(s) 244, 314, 429, 440, 456
MmeI TCCRAC 1 cut(s) 428
MnlI CCTC 1 cut(s) 376
MseI TTAA 3 cut(s) 57, 114, 183
MslI CAYNNNNRTG 1 cut(s) 348
MspA1I CMGCKG 1 cut(s) 258
MspR9I CCNGG 1 cut(s) 138
MvaI CCWGG 1 cut(s) 138
MvnI CGCG 1 cut(s) 258
NdeII GATC 1 cut(s) 172
NlaIII CATG 3 cut(s) 91, 335, 437
NmuCI GTSAC 1 cut(s) 374
PcsI WCGNNNNNNNCGW 1 cut(s) 363
PfeI GAWTC 3 cut(s) 230, 320, 359
PkrI GCNGC 1 cut(s) 30
Psp6I CCWGG 1 cut(s) 136
PspFI CCCAGC 1 cut(s) 64
PspGI CCWGG 1 cut(s) 136
PspPI GGNCC 1 cut(s) 140
PsuI RGATCY 1 cut(s) 172
RsaI GTAC 4 cut(s) 5, 213, 285, 486
RsaNI GTAC 4 cut(s) 4, 212, 284, 485
RseI CAYNNNNRTG 1 cut(s) 348
SacII CCGCGG 1 cut(s) 259
SaqAI TTAA 3 cut(s) 57, 114, 183
SatI GCNGC 1 cut(s) 29
Sau3AI GATC 1 cut(s) 172
Sau96I GGNCC 1 cut(s) 140
ScrFI CCNGG 1 cut(s) 138
SduI GDGCHC 1 cut(s) 170
SetI ASST 7 cut(s) 33, 43, 66, 134, 253, 267, 294
SfaNI GCATC 3 cut(s) 15, 138, 202
Sfr303I CCGCGG 1 cut(s) 259
SgrBI CCGCGG 1 cut(s) 259
SinI GGWCC 1 cut(s) 140
SmiMI CAYNNNNRTG 1 cut(s) 348
SmlI CTYRAG 1 cut(s) 299
SmoI CTYRAG 1 cut(s) 299
Sse9I AATT 5 cut(s) 244, 314, 429, 440, 456
SsiI CCGC 2 cut(s) 256, 258
StyD4I CCNGG 1 cut(s) 136
TaaI ACNGT 3 cut(s) 8, 276, 380
TaiI ACGT 2 cut(s) 43, 294
TaqI TCGA 1 cut(s) 357
TasI AATT 5 cut(s) 244, 314, 429, 440, 456
TatI WGTACW 4 cut(s) 3, 211, 283, 484
TfiI GAWTC 3 cut(s) 230, 320, 359
Tru1I TTAA 3 cut(s) 57, 114, 183
Tru9I TTAA 3 cut(s) 57, 114, 183
TscAI CASTG 2 cut(s) 150, 385
TseFI GTSAC 1 cut(s) 374
TseI GCWGC 1 cut(s) 28
Tsp45I GTSAC 1 cut(s) 374
TspDTI ATGAA 4 cut(s) 76, 99, 366, 422
TspGWI ACGGA 2 cut(s) 165, 434
TspRI CASTG 2 cut(s) 150, 385
VpaK11BI GGWCC 1 cut(s) 140
XapI RAATTY 3 cut(s) 429, 440, 456
XmiI GTMKAC 2 cut(s) 128, 471
ZraI GACGTC 1 cut(s) 292
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.