Rh1BG146600

tRNA (guanine-N(7)-)-methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1B
OG0006250
Physical Location & Seq
Forward (+)
24171971 .. 24184425
12455 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1BG146600.1

Sequence Viewer

Length: 930 bp
ATGGGTTCTTGCGTCTGCTTACGTCAGCACTGCTGCTACGGTGGTTTGGGCGGTGCATTTCAAAGAAGGGGTCTTTCTAGTCTGTTTCGAAAAGGGGTTGTTGCCCCAGGTTTAACGTCGCCGTTTTATCAGTATCAGAGGAACAGAAGAAGAGTGGAGGCCTGTGTTTCTACTGGGTTTAAGGAACAAGAGCTGAGGAGCCCGGACCTTGTGGCCTTGGAATATGCTGATCTTAATCTCACCCACAATGTTTCCCAGGAACTGGGTCACGTCAGAATTAGGCAACATGTGAACCCTCTTAGCTCTTCTTTATCTGTGCCTGTGGAAGTGCCTGATTGGAATCAAGTGTTCAGGGACCCAACATTGCCGCTCATGGTGGACATTGGAAGTGGTAGTGGTAGATTTCTCATGTGGCTGGCGAAAAGAAACTTAGTTGTGAGGAATTATTTGGGACTGGAAATACGGCAGAAACTTGTCAAACGTGCTGATGTCTGGGTTAAGGAGCTGGGTCTTAGTGATATACATTTCATGTATGCAAATGCCACTACTTCATTTAATCAACTGGTGTCTACCTACCCTGGACCACTGATGCTTGTTTCAATCCTGTGCCCAGATCCGTATTTTAAGAGAAAGCATCACAAGAGACGGGTTGTACAGATGCCTTTAGTAGATTCCATTGTGAATAATTTAGCACCCCGCGGACAGGTGTTTATACAGTCCGATGTACTTGACGTCGCACTTGAGATGAGAAACCAATTTGATTCTCTGCCCCATGTTCTGAAGCACATAGATGAAATCGAATCCAGCGTGCTGTGTGACAGAGAGTGTACCAGAAGCAAGTACAGAAGGCTCTTGGATGAAGTAACGCGGAATCAAGTGAATGCAGTATTCAAACTCAAAGAAGTACATCATAATCAGACTCAAAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_4

Protein Analysis

309

Amino Acids

35.35

Weight (kDa)

9.38

Isoelectric Point (pI)

56.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_4 PF02390 123 - 260 5.3e-29 Putative methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 735
AccB7I CCANNNNNTGG 1 cut(s) 262
AccBSI CCGCTC 1 cut(s) 370
AccI GTMKAC 1 cut(s) 569
AccII CGCG 2 cut(s) 699, 868
AciI CCGC 5 cut(s) 51, 368, 697, 699, 868
AclWI GGATC 1 cut(s) 608
AcuI CTGAAG 1 cut(s) 800
AcyI GRCGYC 1 cut(s) 732
AfaI GTAC 5 cut(s) 654, 726, 829, 842, 906
AfiI CCNNNNNNNGG 2 cut(s) 262, 703
AflIII ACRYGT 1 cut(s) 286
AgsI TTSAA 3 cut(s) 62, 600, 892
AjiI CACGTC 1 cut(s) 271
AjnI CCWGG 3 cut(s) 106, 255, 577
AluBI AGCT 3 cut(s) 193, 303, 505
AluI AGCT 3 cut(s) 193, 303, 505
Alw26I GTCTC 1 cut(s) 637
AlwI GGATC 1 cut(s) 608
AlwNI CAGNNNCTG 1 cut(s) 262
AoxI GGCC 2 cut(s) 159, 213
ApeKI GCWGC 1 cut(s) 33
ArsI GACNNNNNNTTYG 2 cut(s) 55, 87
AspS9I GGNCC 3 cut(s) 205, 355, 581
AsuC2I CCSGG 1 cut(s) 203
AsuHPI GGTGA 1 cut(s) 232
AsuII TTCGAA 1 cut(s) 88
AvaII GGWCC 3 cut(s) 205, 355, 581
BaeGI GKGCMC 1 cut(s) 611
BanII GRGCYC 1 cut(s) 203
BbvCI CCTCAGC 1 cut(s) 194
BbvI GCAGC 1 cut(s) 20
BceAI ACGGC 2 cut(s) 106, 479
BciT130I CCWGG 3 cut(s) 108, 257, 579
BcnI CCSGG 1 cut(s) 203
BcoDI GTCTC 1 cut(s) 637
BfaI CTAG 2 cut(s) 78, 928
BisI GCNGC 2 cut(s) 34, 368
BlsI GCNGC 2 cut(s) 35, 369
Bme1390I CCNGG 4 cut(s) 108, 203, 257, 579
Bme18I GGWCC 3 cut(s) 205, 355, 581
BmgBI CACGTC 1 cut(s) 271
BmgT120I GGNCC 3 cut(s) 205, 355, 581
BmiI GGNNCC 3 cut(s) 200, 356, 357
BmrFI CCNGG 4 cut(s) 108, 203, 257, 579
BmrI ACTGGG 2 cut(s) 183, 272
BmsI GCATC 3 cut(s) 579, 643, 648
BmuI ACTGGG 2 cut(s) 183, 272
Bpu10I CCTNAGC 1 cut(s) 194
Bpu14I TTCGAA 1 cut(s) 88
BpuEI CTTGAG 1 cut(s) 761
BpuMI CCSGG 1 cut(s) 203
BsaBI GATNNNNATC 2 cut(s) 234, 339
BsaHI GRCGYC 1 cut(s) 732
BsaJI CCNNGG 5 cut(s) 106, 216, 255, 577, 697
Bsc4I CCNNNNNNNGG 2 cut(s) 262, 703
Bse1I ACTGG 4 cut(s) 178, 267, 459, 567
Bse3DI GCAATG 1 cut(s) 362
Bse8I GATNNNNATC 2 cut(s) 234, 339
BseBI CCWGG 3 cut(s) 108, 257, 579
BseDI CCNNGG 5 cut(s) 106, 216, 255, 577, 697
BseGI GGATG 1 cut(s) 862
BseJI GATNNNNATC 2 cut(s) 234, 339
BseLI CCNNNNNNNGG 2 cut(s) 262, 703
BseMI GCAATG 1 cut(s) 362
BseMII CTCAG 1 cut(s) 185
BseNI ACTGG 4 cut(s) 178, 267, 459, 567
BseRI GAGGAG 1 cut(s) 211
BseSI GKGCMC 1 cut(s) 611
BseXI GCAGC 1 cut(s) 20
BseYI CCCAGC 1 cut(s) 505
Bsh1236I CGCG 2 cut(s) 699, 868
BshFI GGCC 2 cut(s) 161, 215
BsiSI CCGG 1 cut(s) 203
BslFI GGGAC 2 cut(s) 368, 465
BslI CCNNNNNNNGG 2 cut(s) 262, 703
BsmAI GTCTC 1 cut(s) 637
BsmBI CGTCTC 1 cut(s) 637
BsmFI GGGAC 2 cut(s) 368, 465
BsmI GAATGC 1 cut(s) 886
BsnI GGCC 2 cut(s) 161, 215
Bsp119I TTCGAA 1 cut(s) 88
Bsp1286I GDGCHC 2 cut(s) 203, 611
Bsp1407I TGTACA 1 cut(s) 652
Bsp143I GATC 2 cut(s) 229, 613
BspACI CCGC 5 cut(s) 51, 368, 697, 699, 868
BspANI GGCC 2 cut(s) 161, 215
BspCNI CTCAG 1 cut(s) 186
BspFNI CGCG 2 cut(s) 699, 868
BspLI GGNNCC 3 cut(s) 200, 356, 357
BspPI GGATC 1 cut(s) 608
BspQI GCTCTTC 1 cut(s) 310
BspT104I TTCGAA 1 cut(s) 88
BsrBI CCGCTC 1 cut(s) 370
BsrDI GCAATG 1 cut(s) 362
BsrGI TGTACA 1 cut(s) 652
BsrI ACTGG 4 cut(s) 178, 267, 459, 567
BssECI CCNNGG 5 cut(s) 106, 216, 255, 577, 697
BssMI GATC 2 cut(s) 229, 613
BssNI GRCGYC 1 cut(s) 732
BssT1I CCWWGG 1 cut(s) 216
Bst2UI CCWGG 3 cut(s) 108, 257, 579
Bst4CI ACNGT 2 cut(s) 41, 717
Bst6I CTCTTC 2 cut(s) 145, 310
BstACI GRCGYC 1 cut(s) 732
BstAUI TGTACA 1 cut(s) 652
BstBI TTCGAA 1 cut(s) 88
BstC8I GCNNGC 2 cut(s) 417, 809
BstDEI CTNAG 4 cut(s) 194, 299, 430, 512
BstDSI CCRYGG 1 cut(s) 697
BstF5I GGATG 1 cut(s) 862
BstFNI CGCG 2 cut(s) 699, 868
BstKTI GATC 2 cut(s) 232, 616
BstMAI GTCTC 1 cut(s) 637
BstMBI GATC 2 cut(s) 229, 613
BstNI CCWGG 3 cut(s) 108, 257, 579
BstNSI RCATGY 1 cut(s) 290
BstSCI CCNGG 4 cut(s) 106, 201, 255, 577
BstSLI GKGCMC 1 cut(s) 611
BstUI CGCG 2 cut(s) 699, 868
BstV1I GCAGC 1 cut(s) 20
BstX2I RGATCY 1 cut(s) 613
BstYI RGATCY 1 cut(s) 613
BsuRI GGCC 2 cut(s) 161, 215
BtgI CCRYGG 1 cut(s) 697
BtrI CACGTC 1 cut(s) 271
BtsCI GGATG 1 cut(s) 862
BtsI GCAGTG 1 cut(s) 28
BtsIMutI CAGTG 2 cut(s) 28, 584
Cac8I GCNNGC 2 cut(s) 417, 809
CaiI CAGNNNCTG 1 cut(s) 262
Cfr13I GGNCC 3 cut(s) 205, 355, 581
Cfr42I CCGCGG 1 cut(s) 700
Csp6I GTAC 5 cut(s) 653, 725, 828, 841, 905
CviAII CATG 5 cut(s) 287, 373, 409, 529, 773
CviJI RGCY 8 cut(s) 161, 193, 201, 215, 303, 415, 505, 850
CviKI_1 RGCY 8 cut(s) 161, 193, 201, 215, 303, 415, 505, 850
CviQI GTAC 5 cut(s) 653, 725, 828, 841, 905
DdeI CTNAG 4 cut(s) 194, 299, 430, 512
DpnI GATC 2 cut(s) 231, 615
DpnII GATC 2 cut(s) 229, 613
Eam1104I CTCTTC 2 cut(s) 145, 310
EarI CTCTTC 2 cut(s) 145, 310
Eco130I CCWWGG 1 cut(s) 216
Eco147I AGGCCT 1 cut(s) 161
Eco24I GRGCYC 1 cut(s) 203
Eco47I GGWCC 3 cut(s) 205, 355, 581
Eco57I CTGAAG 1 cut(s) 800
EcoO109I RGGNCCY 1 cut(s) 355
EcoRII CCWGG 3 cut(s) 106, 255, 577
EcoT14I CCWWGG 1 cut(s) 216
EcoT38I GRGCYC 1 cut(s) 203
ErhI CCWWGG 1 cut(s) 216
Esp3I CGTCTC 1 cut(s) 637
FaeI CATG 5 cut(s) 290, 376, 412, 532, 776
FaqI GGGAC 2 cut(s) 368, 465
FatI CATG 5 cut(s) 286, 372, 408, 528, 772
FauI CCCGC 1 cut(s) 704
FblI GTMKAC 1 cut(s) 569
Fnu4HI GCNGC 2 cut(s) 34, 368
FokI GGATG 1 cut(s) 869
FriOI GRGCYC 1 cut(s) 203
Fsp4HI GCNGC 2 cut(s) 34, 368
FspBI CTAG 2 cut(s) 78, 928
GluI GCNGC 2 cut(s) 34, 368
GsaI CCCAGC 1 cut(s) 509
HaeIII GGCC 2 cut(s) 161, 215
HapII CCGG 1 cut(s) 203
Hin1I GRCGYC 1 cut(s) 732
Hin1II CATG 5 cut(s) 290, 376, 412, 532, 776
HinfI GANTC 6 cut(s) 340, 671, 761, 800, 871, 919
HpaII CCGG 1 cut(s) 203
HphI GGTGA 1 cut(s) 232
Hpy166II GTNNAC 4 cut(s) 292, 379, 570, 828
Hpy188I TCNGA 5 cut(s) 138, 275, 721, 780, 918
Hpy8I GTNNAC 4 cut(s) 292, 379, 570, 828
Hpy99I CGWCG 2 cut(s) 121, 737
HpyAV CCTTC 2 cut(s) 60, 840
HpyCH4III ACNGT 2 cut(s) 41, 717
HpyCH4IV ACGT 5 cut(s) 22, 116, 270, 481, 732
HpyCH4V TGCA 3 cut(s) 56, 536, 884
HpyF3I CTNAG 4 cut(s) 194, 299, 430, 512
HpySE526I ACGT 5 cut(s) 22, 116, 270, 481, 732
Hsp92I GRCGYC 1 cut(s) 732
Hsp92II CATG 5 cut(s) 290, 376, 412, 532, 776
KflI GGGWCCC 1 cut(s) 355
KspI CCGCGG 1 cut(s) 700
Kzo9I GATC 2 cut(s) 229, 613
LguI GCTCTTC 1 cut(s) 310
LmnI GCTCC 2 cut(s) 198, 502
Lsp1109I GCAGC 1 cut(s) 20
LweI GCATC 3 cut(s) 579, 643, 648
MaeI CTAG 2 cut(s) 78, 928
MaeII ACGT 5 cut(s) 22, 116, 270, 481, 732
MaeIII GTNAC 3 cut(s) 266, 815, 862
MalI GATC 2 cut(s) 231, 615
MbiI CCGCTC 1 cut(s) 370
MboI GATC 2 cut(s) 229, 613
MboII GAAGA 3 cut(s) 159, 162, 297
MflI RGATCY 1 cut(s) 613
MhlI GDGCHC 2 cut(s) 203, 611
MluCI AATT 4 cut(s) 276, 442, 685, 755
MlyI GAGTC 1 cut(s) 913
MnlI CCTC 5 cut(s) 132, 151, 189, 306, 432
MseI TTAA 6 cut(s) 113, 180, 234, 498, 555, 624
MslI CAYNNNNRTG 1 cut(s) 789
MspA1I CMGCKG 1 cut(s) 699
MspI CCGG 1 cut(s) 203
MspR9I CCNGG 4 cut(s) 108, 203, 257, 579
Mva1269I GAATGC 1 cut(s) 886
MvaI CCWGG 3 cut(s) 108, 257, 579
MvnI CGCG 2 cut(s) 699, 868
NciI CCSGG 1 cut(s) 203
NdeII GATC 2 cut(s) 229, 613
NlaIII CATG 5 cut(s) 290, 376, 412, 532, 776
NlaIV GGNNCC 3 cut(s) 200, 356, 357
NmuCI GTSAC 2 cut(s) 266, 815
NspI RCATGY 1 cut(s) 290
NspV TTCGAA 1 cut(s) 88
PceI AGGCCT 1 cut(s) 161
PciI ACATGT 1 cut(s) 286
PciSI GCTCTTC 1 cut(s) 310
PcsI WCGNNNNNNNCGW 1 cut(s) 804
PctI GAATGC 1 cut(s) 886
PfeI GAWTC 5 cut(s) 340, 671, 761, 800, 871
PflMI CCANNNNNTGG 1 cut(s) 262
PkrI GCNGC 2 cut(s) 35, 369
PleI GAGTC 1 cut(s) 913
PpsI GAGTC 1 cut(s) 913
PpuMI RGGWCCY 1 cut(s) 355
PscI ACATGT 1 cut(s) 286
Psp5II RGGWCCY 1 cut(s) 355
Psp6I CCWGG 3 cut(s) 106, 255, 577
PspFI CCCAGC 1 cut(s) 505
PspGI CCWGG 3 cut(s) 106, 255, 577
PspN4I GGNNCC 3 cut(s) 200, 356, 357
PspPI GGNCC 3 cut(s) 205, 355, 581
PspPPI RGGWCCY 1 cut(s) 355
PstNI CAGNNNCTG 1 cut(s) 262
PsuI RGATCY 1 cut(s) 613
RsaI GTAC 5 cut(s) 654, 726, 829, 842, 906
RsaNI GTAC 5 cut(s) 653, 725, 828, 841, 905
RseI CAYNNNNRTG 1 cut(s) 789
SacII CCGCGG 1 cut(s) 700
SapI GCTCTTC 1 cut(s) 310
SaqAI TTAA 6 cut(s) 113, 180, 234, 498, 555, 624
SatI GCNGC 2 cut(s) 34, 368
Sau3AI GATC 2 cut(s) 229, 613
Sau96I GGNCC 3 cut(s) 205, 355, 581
SchI GAGTC 1 cut(s) 913
ScrFI CCNGG 4 cut(s) 108, 203, 257, 579
SduI GDGCHC 2 cut(s) 203, 611
SfaNI GCATC 3 cut(s) 579, 643, 648
Sfr303I CCGCGG 1 cut(s) 700
SfuI TTCGAA 1 cut(s) 88
SgrBI CCGCGG 1 cut(s) 700
SinI GGWCC 3 cut(s) 205, 355, 581
SmiMI CAYNNNNRTG 1 cut(s) 789
SmlI CTYRAG 1 cut(s) 740
SmoI CTYRAG 1 cut(s) 740
Sse9I AATT 4 cut(s) 276, 442, 685, 755
SseBI AGGCCT 1 cut(s) 161
SsiI CCGC 5 cut(s) 51, 368, 697, 699, 868
SspMI CTAG 2 cut(s) 78, 928
StuI AGGCCT 1 cut(s) 161
StyD4I CCNGG 4 cut(s) 106, 201, 255, 577
StyI CCWWGG 1 cut(s) 216
TaaI ACNGT 2 cut(s) 41, 717
TaiI ACGT 5 cut(s) 25, 119, 273, 484, 735
TaqI TCGA 2 cut(s) 88, 798
TasI AATT 4 cut(s) 276, 442, 685, 755
TatI WGTACW 4 cut(s) 652, 724, 840, 904
TauI GCSGC 1 cut(s) 370
TfiI GAWTC 5 cut(s) 340, 671, 761, 800, 871
Tru1I TTAA 6 cut(s) 113, 180, 234, 498, 555, 624
Tru9I TTAA 6 cut(s) 113, 180, 234, 498, 555, 624
TscAI CASTG 2 cut(s) 35, 591
TseFI GTSAC 2 cut(s) 266, 815
TseI GCWGC 1 cut(s) 33
Tsp45I GTSAC 2 cut(s) 266, 815
TspDTI ATGAA 4 cut(s) 517, 540, 807, 873
TspGWI ACGGA 1 cut(s) 606
TspRI CASTG 2 cut(s) 35, 591
Van91I CCANNNNNTGG 1 cut(s) 262
VpaK11BI GGWCC 3 cut(s) 205, 355, 581
XceI RCATGY 1 cut(s) 290
XmiI GTMKAC 1 cut(s) 569
XspI CTAG 2 cut(s) 78, 928
ZraI GACGTC 1 cut(s) 733
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.