FvH4_7g25420

38.1 kDa protein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0006546
Physical Location & Seq
Forward (+)
19410186 .. 19412896
2711 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g25420.t1

Sequence Viewer

Length: 1005 bp
ATGGGAAACGCATTGAGGCTTCTTTACGGTCATTGCTGCAAGCCCACCACCACTGAATCCGACTCTCTTGGCCCCCACGGCGTTTCCGCCGCCACTGTCGGAGTTTCAGCTCTTGCCCGCGATCTCTTCCACTTTGAGAACACCGCCCAGGTCCCAGTAGGACTGAATCAGCATGTAGTTTCATCCAAGAAAGCTCAAGCTAATTGGTATAGAAAACTAGTTGAGAGCTGGAGGGAAGCGAGACCACCACCCAAGACAGCTGAAGAAGCAGCTAGGCTTGTTATTAAAACCCTCAAGAATCACCAAAAGGCTGATGTTGAGGGATTATTGGCTTTCTATGGTCTGCCACTTCCTCAAACTCTGGTCCAACTTTCGACTGGGGTCCCAACTTCATTGCCTCAAGGAGTACAGTATGAATTCCAGACATTACCGGTTGATGCAAAAGCAGTTGCAGATGGCGATACAGTGACAGTCTATGTGAGTACAGCAGATCCTAGGGAGTCATCTAATGTTCCGACTGAGGTACAAGCAGCAATCATCCAAAGAGAAAAGGCACGCGAGAAGAGGAATTATGCCAAGGCAGATGAACTTCACAAAAAGATCATTGATGCTGGATACCGGGTACTACAGTTTCAAAACGAGGATGTCCTTGCGCGGAAGTATAGGATTCGACTGAGGGGAATAGATGCACCAGAGAGTAAAATGCCTTATGGAAAAGAATCCAAAGATGAGCTTGTTAAGCTTCTTCAGGGCAAGTGTTTGAGGGTCCTTGTCTACGGGACAGATCGTTATGGCCGTTGTGTAGGTGACATATACAGCAATGGCATATTTGCACAGGAAGTAATGCTGAAGAAGGGGTGTGCATGGCATTACACAGCCTATGACCAACGACCGGAACTTGCAAAATGGGAAAGAGAAGCTCAAATGAAGCGAGTTGGCTTATGGGCTTCATCAAACCCTGAGAAGCCATGGGAATGGAGAAAGGATAGACGTGAAGGCAGATGA

Protein Analysis

335

Amino Acids

37.65

Weight (kDa)

9.3

Isoelectric Point (pI)

29.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SNase PF00565 222 - 316 4.8e-25 Staphylococcal nuclease homologue
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 774
AccII CGCG 3 cut(s) 120, 558, 655
AciI CCGC 5 cut(s) 87, 90, 118, 144, 655
AclWI GGATC 1 cut(s) 485
AcoI YGGCCR 1 cut(s) 793
AcsI RAATTY 1 cut(s) 416
AcuI CTGAAG 3 cut(s) 282, 731, 869
AfaI GTAC 4 cut(s) 408, 484, 525, 624
AfiI CCNNNNNNNGG 1 cut(s) 892
AgeI ACCGGT 1 cut(s) 430
AgsI TTSAA 1 cut(s) 635
AhlI ACTAGT 1 cut(s) 217
AjiI CACGTC 1 cut(s) 992
AjnI CCWGG 1 cut(s) 147
AluBI AGCT 9 cut(s) 110, 194, 200, 228, 260, 272, 733, 742, 920
AluI AGCT 9 cut(s) 110, 194, 200, 228, 260, 272, 733, 742, 920
Alw26I GTCTC 1 cut(s) 235
AlwI GGATC 1 cut(s) 485
AoxI GGCC 2 cut(s) 70, 793
ApeKI GCWGC 3 cut(s) 36, 269, 530
ApoI RAATTY 1 cut(s) 416
AsiGI ACCGGT 1 cut(s) 430
AspA2I CCTAGG 1 cut(s) 494
AspLEI GCGC 1 cut(s) 655
AspS9I GGNCC 5 cut(s) 71, 151, 364, 382, 766
AsuC2I CCSGG 1 cut(s) 620
AsuHPI GGTGA 2 cut(s) 293, 818
AvaII GGWCC 4 cut(s) 151, 364, 382, 766
AvrII CCTAGG 1 cut(s) 494
BaeI ACNNNNGTAYC 2 cut(s) 614, 647
BbvI GCAGC 3 cut(s) 23, 281, 542
BccI CCATC 1 cut(s) 449
BceAI ACGGC 2 cut(s) 94, 780
BciT130I CCWGG 1 cut(s) 149
BciVI GTATCC 1 cut(s) 608
BcnI CCSGG 1 cut(s) 620
BcoDI GTCTC 1 cut(s) 235
BcuI ACTAGT 1 cut(s) 217
BfaI CTAG 3 cut(s) 218, 273, 495
BfmI CTRYAG 1 cut(s) 626
BfuI GTATCC 1 cut(s) 608
BglI GCCNNNNNGGC 1 cut(s) 78
BisI GCNGC 4 cut(s) 37, 90, 270, 531
BlnI CCTAGG 1 cut(s) 494
BlsI GCNGC 4 cut(s) 38, 91, 271, 532
Bme1390I CCNGG 2 cut(s) 149, 620
Bme18I GGWCC 4 cut(s) 151, 364, 382, 766
BmgBI CACGTC 1 cut(s) 992
BmgT120I GGNCC 5 cut(s) 71, 151, 364, 382, 766
BmiI GGNNCC 5 cut(s) 73, 153, 383, 384, 767
BmrFI CCNGG 2 cut(s) 149, 620
BmrI ACTGGG 2 cut(s) 149, 387
BmsI GCATC 3 cut(s) 427, 598, 676
BmuI ACTGGG 2 cut(s) 149, 387
BoxI GACNNNNGTC 1 cut(s) 380
BpmI CTGGAG 1 cut(s) 250
BpuEI CTTGAG 3 cut(s) 180, 278, 384
BpuMI CCSGG 1 cut(s) 620
BsaI GGTCTC 1 cut(s) 235
BsaJI CCNNGG 5 cut(s) 76, 147, 494, 576, 968
BsaWI WCCGGW 2 cut(s) 430, 892
Bsc4I CCNNNNNNNGG 1 cut(s) 892
Bse118I RCCGGY 1 cut(s) 430
Bse1I ACTGG 2 cut(s) 155, 382
Bse3DI GCAATG 3 cut(s) 31, 392, 826
BseBI CCWGG 1 cut(s) 149
BseDI CCNNGG 5 cut(s) 76, 147, 494, 576, 968
BseGI GGATG 3 cut(s) 182, 537, 649
BseLI CCNNNNNNNGG 1 cut(s) 892
BseMI GCAATG 3 cut(s) 31, 392, 826
BseMII CTCAG 3 cut(s) 510, 665, 951
BseNI ACTGG 2 cut(s) 155, 382
BseXI GCAGC 3 cut(s) 23, 281, 542
Bsh1236I CGCG 3 cut(s) 120, 558, 655
Bsh1285I CGRYCG 1 cut(s) 893
BshFI GGCC 2 cut(s) 72, 795
BshTI ACCGGT 1 cut(s) 430
BsiEI CGRYCG 1 cut(s) 893
BsiSI CCGG 3 cut(s) 431, 619, 893
BslFI GGGAC 3 cut(s) 137, 368, 793
BslI CCNNNNNNNGG 1 cut(s) 892
BsmAI GTCTC 1 cut(s) 235
BsmFI GGGAC 3 cut(s) 137, 368, 793
BsnI GGCC 2 cut(s) 72, 795
Bso31I GGTCTC 1 cut(s) 235
Bsp143I GATC 4 cut(s) 121, 490, 600, 784
Bsp19I CCATGG 1 cut(s) 968
BspACI CCGC 5 cut(s) 87, 90, 118, 144, 655
BspANI GGCC 2 cut(s) 72, 795
BspCNI CTCAG 3 cut(s) 511, 666, 952
BspFNI CGCG 3 cut(s) 120, 558, 655
BspLI GGNNCC 5 cut(s) 73, 153, 383, 384, 767
BspPI GGATC 1 cut(s) 485
BspTNI GGTCTC 1 cut(s) 235
BsrDI GCAATG 3 cut(s) 31, 392, 826
BsrFI RCCGGY 1 cut(s) 430
BsrI ACTGG 2 cut(s) 155, 382
BssAI RCCGGY 1 cut(s) 430
BssECI CCNNGG 5 cut(s) 76, 147, 494, 576, 968
BssMI GATC 4 cut(s) 121, 490, 600, 784
BssT1I CCWWGG 3 cut(s) 494, 576, 968
Bst2UI CCWGG 1 cut(s) 149
Bst4CI ACNGT 6 cut(s) 29, 97, 411, 466, 472, 630
Bst6I CTCTTC 2 cut(s) 131, 557
BstC8I GCNNGC 3 cut(s) 41, 118, 556
BstDEI CTNAG 3 cut(s) 519, 674, 960
BstDSI CCRYGG 2 cut(s) 76, 968
BstF5I GGATG 3 cut(s) 182, 537, 649
BstFNI CGCG 3 cut(s) 120, 558, 655
BstHHI GCGC 1 cut(s) 655
BstKTI GATC 4 cut(s) 124, 493, 603, 787
BstMAI GTCTC 1 cut(s) 235
BstMBI GATC 4 cut(s) 121, 490, 600, 784
BstMCI CGRYCG 1 cut(s) 893
BstMWI GCNNNNNNNGC 3 cut(s) 78, 266, 739
BstNI CCWGG 1 cut(s) 149
BstNSI RCATGY 1 cut(s) 176
BstPAI GACNNNNGTC 1 cut(s) 380
BstSCI CCNGG 2 cut(s) 147, 618
BstSFI CTRYAG 1 cut(s) 626
BstUI CGCG 3 cut(s) 120, 558, 655
BstV1I GCAGC 3 cut(s) 23, 281, 542
BstX2I RGATCY 1 cut(s) 490
BstXI CCANNNNNNTGG 1 cut(s) 975
BstYI RGATCY 1 cut(s) 490
BsuI GTATCC 1 cut(s) 608
BsuRI GGCC 2 cut(s) 72, 795
BtgI CCRYGG 2 cut(s) 76, 968
BtrI CACGTC 1 cut(s) 992
BtsCI GGATG 3 cut(s) 182, 537, 649
BtsIMutI CAGTG 3 cut(s) 51, 93, 471
Cac8I GCNNGC 3 cut(s) 41, 118, 556
CfoI GCGC 1 cut(s) 655
Cfr10I RCCGGY 1 cut(s) 430
Cfr13I GGNCC 5 cut(s) 71, 151, 364, 382, 766
Csp6I GTAC 4 cut(s) 407, 483, 524, 623
CspAI ACCGGT 1 cut(s) 430
CviAII CATG 3 cut(s) 173, 864, 969
CviQI GTAC 4 cut(s) 407, 483, 524, 623
DdeI CTNAG 3 cut(s) 519, 674, 960
DpnI GATC 4 cut(s) 123, 492, 602, 786
DpnII GATC 4 cut(s) 121, 490, 600, 784
EaeI YGGCCR 1 cut(s) 793
Eam1104I CTCTTC 2 cut(s) 131, 557
EarI CTCTTC 2 cut(s) 131, 557
EciI GGCGGA 1 cut(s) 76
Eco130I CCWWGG 3 cut(s) 494, 576, 968
Eco31I GGTCTC 1 cut(s) 235
Eco47I GGWCC 4 cut(s) 151, 364, 382, 766
Eco57I CTGAAG 3 cut(s) 282, 731, 869
EcoO109I RGGNCCY 3 cut(s) 151, 382, 766
EcoRI GAATTC 1 cut(s) 416
EcoRII CCWGG 1 cut(s) 147
EcoT14I CCWWGG 3 cut(s) 494, 576, 968
ErhI CCWWGG 3 cut(s) 494, 576, 968
FaeI CATG 3 cut(s) 176, 867, 972
FalI AAGNNNNNCTT 2 cut(s) 717, 749
FaqI GGGAC 3 cut(s) 137, 368, 793
FatI CATG 3 cut(s) 172, 863, 968
FauI CCCGC 1 cut(s) 125
FblI GTMKAC 1 cut(s) 774
Fnu4HI GCNGC 4 cut(s) 37, 90, 270, 531
FokI GGATG 3 cut(s) 169, 524, 656
Fsp4HI GCNGC 4 cut(s) 37, 90, 270, 531
FspBI CTAG 3 cut(s) 218, 273, 495
GlaI GCGC 1 cut(s) 654
GluI GCNGC 4 cut(s) 37, 90, 270, 531
GsuI CTGGAG 1 cut(s) 250
HaeIII GGCC 2 cut(s) 72, 795
HapII CCGG 3 cut(s) 431, 619, 893
HhaI GCGC 1 cut(s) 655
Hin1II CATG 3 cut(s) 176, 867, 972
Hin6I GCGC 1 cut(s) 653
HinP1I GCGC 1 cut(s) 653
HindIII AAGCTT 1 cut(s) 740
HinfI GANTC 7 cut(s) 56, 62, 166, 298, 500, 667, 719
HpaII CCGG 3 cut(s) 431, 619, 893
HphI GGTGA 2 cut(s) 293, 818
Hpy166II GTNNAC 1 cut(s) 775
Hpy188I TCNGA 3 cut(s) 61, 101, 516
Hpy188III TCNNGA 2 cut(s) 295, 421
Hpy8I GTNNAC 1 cut(s) 775
HpyAV CCTTC 2 cut(s) 847, 989
HpyCH4III ACNGT 6 cut(s) 29, 97, 411, 466, 472, 630
HpyCH4IV ACGT 1 cut(s) 991
HpyCH4V TGCA 7 cut(s) 39, 440, 452, 689, 833, 863, 902
HpyF10VI GCNNNNNNNGC 3 cut(s) 78, 266, 739
HpyF3I CTNAG 3 cut(s) 519, 674, 960
HpySE526I ACGT 1 cut(s) 991
Hsp92II CATG 3 cut(s) 176, 867, 972
HspAI GCGC 1 cut(s) 653
KflI GGGWCCC 1 cut(s) 382
Kzo9I GATC 4 cut(s) 121, 490, 600, 784
Lsp1109I GCAGC 3 cut(s) 23, 281, 542
LweI GCATC 3 cut(s) 427, 598, 676
MaeI CTAG 3 cut(s) 218, 273, 495
MaeII ACGT 1 cut(s) 991
MaeIII GTNAC 2 cut(s) 466, 806
MalI GATC 4 cut(s) 123, 492, 602, 786
MboI GATC 4 cut(s) 121, 490, 600, 784
MboII GAAGA 5 cut(s) 118, 275, 574, 737, 862
MflI RGATCY 1 cut(s) 490
MluCI AATT 3 cut(s) 202, 416, 568
MlyI GAGTC 2 cut(s) 56, 509
MmeI TCCRAC 4 cut(s) 79, 84, 391, 539
MseI TTAA 2 cut(s) 285, 738
MslI CAYNNNNRTG 1 cut(s) 973
MspA1I CMGCKG 1 cut(s) 260
MspI CCGG 3 cut(s) 431, 619, 893
MspR9I CCNGG 2 cut(s) 149, 620
MvaI CCWGG 1 cut(s) 149
MvnI CGCG 3 cut(s) 120, 558, 655
MwoI GCNNNNNNNGC 3 cut(s) 78, 266, 739
NciI CCSGG 1 cut(s) 620
NcoI CCATGG 1 cut(s) 968
NdeII GATC 4 cut(s) 121, 490, 600, 784
NlaIII CATG 3 cut(s) 176, 867, 972
NlaIV GGNNCC 5 cut(s) 73, 153, 383, 384, 767
NmuCI GTSAC 2 cut(s) 466, 806
NspI RCATGY 1 cut(s) 176
PcsI WCGNNNNNNNCGW 1 cut(s) 793
PfeI GAWTC 5 cut(s) 56, 166, 298, 667, 719
PinAI ACCGGT 1 cut(s) 430
PkrI GCNGC 4 cut(s) 38, 91, 271, 532
PleI GAGTC 2 cut(s) 56, 508
PpsI GAGTC 2 cut(s) 56, 508
PpuMI RGGWCCY 3 cut(s) 151, 382, 766
PshAI GACNNNNGTC 1 cut(s) 380
Psp5II RGGWCCY 3 cut(s) 151, 382, 766
Psp6I CCWGG 1 cut(s) 147
PspGI CCWGG 1 cut(s) 147
PspN4I GGNNCC 5 cut(s) 73, 153, 383, 384, 767
PspPI GGNCC 5 cut(s) 71, 151, 364, 382, 766
PspPPI RGGWCCY 3 cut(s) 151, 382, 766
PsuI RGATCY 1 cut(s) 490
PvuII CAGCTG 1 cut(s) 260
RsaI GTAC 4 cut(s) 408, 484, 525, 624
RsaNI GTAC 4 cut(s) 407, 483, 524, 623
RseI CAYNNNNRTG 1 cut(s) 973
SaqAI TTAA 2 cut(s) 285, 738
SatI GCNGC 4 cut(s) 37, 90, 270, 531
Sau3AI GATC 4 cut(s) 121, 490, 600, 784
Sau96I GGNCC 5 cut(s) 71, 151, 364, 382, 766
SchI GAGTC 2 cut(s) 56, 509
ScrFI CCNGG 2 cut(s) 149, 620
SfaNI GCATC 3 cut(s) 427, 598, 676
SfcI CTRYAG 1 cut(s) 626
SinI GGWCC 4 cut(s) 151, 364, 382, 766
SmiMI CAYNNNNRTG 1 cut(s) 973
SmlI CTYRAG 3 cut(s) 195, 293, 399
SmoI CTYRAG 3 cut(s) 195, 293, 399
SpeI ACTAGT 1 cut(s) 217
Sse9I AATT 3 cut(s) 202, 416, 568
SsiI CCGC 5 cut(s) 87, 90, 118, 144, 655
SspMI CTAG 3 cut(s) 218, 273, 495
StyD4I CCNGG 2 cut(s) 147, 618
StyI CCWWGG 3 cut(s) 494, 576, 968
TaaI ACNGT 6 cut(s) 29, 97, 411, 466, 472, 630
TaiI ACGT 1 cut(s) 994
TaqI TCGA 2 cut(s) 374, 670
TasI AATT 3 cut(s) 202, 416, 568
TatI WGTACW 2 cut(s) 406, 482
TauI GCSGC 1 cut(s) 92
TfiI GAWTC 5 cut(s) 56, 166, 298, 667, 719
Tru1I TTAA 2 cut(s) 285, 738
Tru9I TTAA 2 cut(s) 285, 738
TscAI CASTG 3 cut(s) 58, 100, 471
TseFI GTSAC 2 cut(s) 466, 806
TseI GCWGC 3 cut(s) 36, 269, 530
Tsp45I GTSAC 2 cut(s) 466, 806
TspDTI ATGAA 6 cut(s) 171, 381, 429, 600, 939, 941
TspRI CASTG 3 cut(s) 58, 100, 471
VpaK11BI GGWCC 4 cut(s) 151, 364, 382, 766
XapI RAATTY 1 cut(s) 416
XceI RCATGY 1 cut(s) 176
XcmI CCANNNNNNNNNTGG 1 cut(s) 374
XmaJI CCTAGG 1 cut(s) 494
XmiI GTMKAC 1 cut(s) 774
XspI CTAG 3 cut(s) 218, 273, 495
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.