Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0006546

Physical Location & Seq

Genomic Coordinates Forward (+)

26631937 .. 26634443

Gene Structure 2507 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1160800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1002 bp

ATGGGGTTGTTGGGAAGCTTCGTAAGGTTCCTCTTGGGCCAATGCAGCAAACCCACCACCACCGGCGGGGACTACTCTGATCCACTTGGCCATCACGGCGTCTCCGCCACCACTGTCGGCGTTTCGGCTCTTGCCCAAGACCTCTACAACTTCGAGATCACCTCTCAGGTCCCAGAAGGGCTCGGTCAGCATGTTGCCTCGTCCAGGAAAGCTCAAGCTAACTGGTATAGAAAACTAGTTGTGACATGGAGGGAAGCAAAACCGCCACCAAGAACACCCGAAGAAGCGGCTAGTCTTGTCATACAAACCCTGAAAGGACATCGAAAGGCGGATGTTGAGGGTGTGTTGGCTTTCTATGGCCTCAATATTTCCTCTGAGGATCCAACATCATTGCCTCAAGGAGTAAAGTTTGAGTTCCAGACGCTACCGGTTGATGCAAAAGCGATTGCAGATGGCGATACAGTGACAGTCTATGTCAGCACAGAGGATCCCAGGGAGTCATCCTGTGTTCCGAGCGATGTGCAAACAGCTGCCATTGAAAGATCAGAAGCACGCGCAGTGAGGAACTATACAAGGGCAGATGCACTTCACAAGAAGATCATTGATGCTGGATACCGGGTACTGAATATTGAAAACGAGGAGGTCCTTGCCCGAAAGTACAGGATCCGACTGAGGGGTATAGATGCGCCGGAGAGTTCAATGCCCTATGGAAAAGAAGCCAAACAGGAGCTGGTTAAGCTTATTCAGGGCAAGTGCTTGAGGGTTCTTATCTACGGGGAAGATCGTTATGGACGCTGTGTGGGCGACATATACAGCAATGGCACATTTGTACAGGAAGTAATGCTCAAGAACGGGTGTGCATGGCATTACGCAGCCTACGACAAACGCCCAGAATTTGCAAGGTGGGAAGAACAGGCTCGGGCGGAGCGAGTTGGGTTGTGGGCTTCATCAAACCCTGAGAAGCCATGGGATTGGAGAAAGGATAAGCGTCAAGGCAAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004518 GO:0004519 GO:0004520 GO:0004521 GO:0004536 GO:0004540 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006139 GO:0006259 GO:0006308 GO:0006401 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0019439 GO:0034641 GO:0034655 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044248 GO:0044260 GO:0044265 GO:0044270 GO:0044424 GO:0044464 GO:0046483 GO:0046700 GO:0071704 GO:0090304 GO:0090305 GO:0090501 GO:0090502 GO:0140097 GO:0140098 GO:1901360 GO:1901361 GO:1901575

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

SNase

Protein Analysis

334

Amino Acids

37.18

Weight (kDa)

8.45

Isoelectric Point (pI)

40.02

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
SNase	PF00565	221 - 315	5.3e-27	Staphylococcal nuclease homologue

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006546)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G40410 AT2G40410
fragaria_vesca	FvH4_7g25420 FvH4_7g25420 FvH4_7g25420
malus_domestica	MD01G1160800.v1.1 MD07G1228600.v1.1
prunus_persica	Prupe.2G258500_v2.0.a1
pyrus_communis	pycom01g17670 pycom07g20700
rosa_chinensis	RchiOBHm_Chr1g0371391 RchiOBHm_Chr3g0455981
rosa_laevigata	RLG00000026967 RLG00000030163
rosa_multiflora	Rmu_co8299661.1_g000001 Rmu_sc0000809.1_g000026 Rmu_sc0027221.1_g000001
rosa_roxburghii	Rroxscaffold_3G00232720 Rroxscaffold_4G00285730
rosa_rugosa	Rorug01G0363600
rosa_samantha	Rh1AG373700 Rh1BG337100 Rh1DG368100 Rh5BG126800
rosa_wichuraiana	Rw1G032870 Rw3G017510

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccII	CGCG	1 cut(s)	555
AciI	CCGC	6 cut(s)	66, 105, 263, 287, 329, 923
AclWI	GGATC	7 cut(s)	74, 374, 387, 482, 495, 658, 671
AcoI	YGGCCR	1 cut(s)	88
AcsI	RAATTY	1 cut(s)	893
AcyI	GRCGYC	1 cut(s)	99
AfaI	GTAC	3 cut(s)	621, 659, 831
AfiI	CCNNNNNNNGG	3 cut(s)	66, 204, 673
AgeI	ACCGGT	1 cut(s)	427
AgsI	TTSAA	3 cut(s)	539, 632, 699
AhlI	ACTAGT	1 cut(s)	235
AjnI	CCWGG	2 cut(s)	203, 491
AluBI	AGCT	6 cut(s)	18, 212, 218, 530, 730, 739
AluI	AGCT	6 cut(s)	18, 212, 218, 530, 730, 739
Alw26I	GTCTC	1 cut(s)	106
AlwI	GGATC	7 cut(s)	74, 374, 387, 482, 495, 658, 671
AlwNI	CAGNNNCTG	1 cut(s)	730
Ama87I	CYCGRG	1 cut(s)	918
AoxI	GGCC	3 cut(s)	37, 88, 358
ApeKI	GCWGC	3 cut(s)	45, 530, 872
ApoI	RAATTY	1 cut(s)	893
AsiGI	ACCGGT	1 cut(s)	427
AspLEI	GCGC	2 cut(s)	557, 688
AspS9I	GGNCC	3 cut(s)	37, 169, 643
AsuC2I	CCSGG	1 cut(s)	617
AsuHPI	GGTGA	1 cut(s)	151
AvaI	CYCGRG	1 cut(s)	918
AvaII	GGWCC	2 cut(s)	169, 643
BalI	TGGCCA	1 cut(s)	90
BamHI	GGATCC	3 cut(s)	379, 487, 663
BanII	GRGCYC	1 cut(s)	183
BbvI	GCAGC	3 cut(s)	57, 517, 884
BccI	CCATC	2 cut(s)	99, 446
BceAI	ACGGC	1 cut(s)	112
BcgI	CGANNNNNNTGC	2 cut(s)	502, 536
BciT130I	CCWGG	2 cut(s)	205, 493
BciVI	GTATCC	1 cut(s)	605
BcnI	CCSGG	1 cut(s)	617
BcoDI	GTCTC	1 cut(s)	106
BcuI	ACTAGT	1 cut(s)	235
BfaI	CTAG	2 cut(s)	236, 291
BfuI	GTATCC	1 cut(s)	605
BglI	GCCNNNNNGGC	1 cut(s)	96
BisI	GCNGC	4 cut(s)	46, 288, 531, 873
BlsI	GCNGC	4 cut(s)	47, 289, 532, 874
Bme1390I	CCNGG	3 cut(s)	205, 493, 617
Bme18I	GGWCC	2 cut(s)	169, 643
BmeT110I	CYCGRG	1 cut(s)	918
BmgT120I	GGNCC	3 cut(s)	37, 169, 643
BmiI	GGNNCC	5 cut(s)	29, 171, 381, 489, 665
BmrFI	CCNGG	3 cut(s)	205, 493, 617
BmsI	GCATC	4 cut(s)	424, 571, 595, 673
BplI	GAGNNNNNCTC	2 cut(s)	146, 178
BpuEI	CTTGAG	4 cut(s)	198, 381, 778, 830
BpuMI	CCSGG	1 cut(s)	617
BsaHI	GRCGYC	1 cut(s)	99
BsaJI	CCNNGG	3 cut(s)	491, 492, 965
BsaWI	WCCGGW	1 cut(s)	427
BsaXI	ACNNNNNCTCC	2 cut(s)	86, 116
Bsc4I	CCNNNNNNNGG	3 cut(s)	66, 204, 673
Bse118I	RCCGGY	2 cut(s)	62, 427
Bse1I	ACTGG	1 cut(s)	227
Bse3DI	GCAATG	2 cut(s)	389, 823
BseBI	CCWGG	2 cut(s)	205, 493
BseDI	CCNNGG	3 cut(s)	491, 492, 965
BseGI	GGATG	2 cut(s)	337, 500
BseLI	CCNNNNNNNGG	3 cut(s)	66, 204, 673
BseMI	GCAATG	2 cut(s)	389, 823
BseMII	CTCAG	4 cut(s)	179, 366, 662, 948
BseNI	ACTGG	1 cut(s)	227
BseRI	GAGGAG	1 cut(s)	653
BseXI	GCAGC	3 cut(s)	57, 517, 884
Bsh1236I	CGCG	1 cut(s)	555
BshFI	GGCC	3 cut(s)	39, 90, 360
BshTI	ACCGGT	1 cut(s)	427
BsiHKCI	CYCGRG	1 cut(s)	918
BsiSI	CCGG	4 cut(s)	63, 428, 616, 689
BslFI	GGGAC	2 cut(s)	83, 155
BslI	CCNNNNNNNGG	3 cut(s)	66, 204, 673
BsmAI	GTCTC	1 cut(s)	106
BsmBI	CGTCTC	1 cut(s)	106
BsmFI	GGGAC	2 cut(s)	83, 155
BsnI	GGCC	3 cut(s)	39, 90, 360
BsoBI	CYCGRG	1 cut(s)	918
Bsp1286I	GDGCHC	1 cut(s)	183
Bsp1407I	TGTACA	1 cut(s)	829
Bsp143I	GATC	8 cut(s)	79, 156, 379, 487, 542, 597, 663, 781
Bsp19I	CCATGG	1 cut(s)	965
BspACI	CCGC	6 cut(s)	66, 105, 263, 287, 329, 923
BspANI	GGCC	3 cut(s)	39, 90, 360
BspCNI	CTCAG	4 cut(s)	178, 367, 663, 949
BspFNI	CGCG	1 cut(s)	555
BspLI	GGNNCC	5 cut(s)	29, 171, 381, 489, 665
BspPI	GGATC	7 cut(s)	74, 374, 387, 482, 495, 658, 671
BsrDI	GCAATG	2 cut(s)	389, 823
BsrFI	RCCGGY	2 cut(s)	62, 427
BsrGI	TGTACA	1 cut(s)	829
BsrI	ACTGG	1 cut(s)	227
BssAI	RCCGGY	2 cut(s)	62, 427
BssECI	CCNNGG	3 cut(s)	491, 492, 965
BssMI	GATC	8 cut(s)	79, 156, 379, 487, 542, 597, 663, 781
BssNI	GRCGYC	1 cut(s)	99
BssT1I	CCWWGG	1 cut(s)	965
Bst2UI	CCWGG	2 cut(s)	205, 493
Bst4CI	ACNGT	3 cut(s)	115, 463, 469
BstACI	GRCGYC	1 cut(s)	99
BstAUI	TGTACA	1 cut(s)	829
BstC8I	GCNNGC	1 cut(s)	553
BstDEI	CTNAG	4 cut(s)	165, 375, 671, 957
BstDSI	CCRYGG	1 cut(s)	965
BstENI	CCTNNNNNAGG	1 cut(s)	202
BstF5I	GGATG	2 cut(s)	337, 500
BstFNI	CGCG	1 cut(s)	555
BstHHI	GCGC	2 cut(s)	557, 688
BstKTI	GATC	8 cut(s)	82, 159, 382, 490, 545, 600, 666, 784
BstMAI	GTCTC	1 cut(s)	106
BstMBI	GATC	8 cut(s)	79, 156, 379, 487, 542, 597, 663, 781
BstMWI	GCNNNNNNNGC	5 cut(s)	45, 96, 187, 736, 801
BstNI	CCWGG	2 cut(s)	205, 493
BstNSI	RCATGY	1 cut(s)	194
BstSCI	CCNGG	3 cut(s)	203, 491, 615
BstUI	CGCG	1 cut(s)	555
BstV1I	GCAGC	3 cut(s)	57, 517, 884
BstX2I	RGATCY	3 cut(s)	379, 487, 663
BstXI	CCANNNNNNTGG	1 cut(s)	972
BstYI	RGATCY	3 cut(s)	379, 487, 663
BsuI	GTATCC	1 cut(s)	605
BsuRI	GGCC	3 cut(s)	39, 90, 360
BtgI	CCRYGG	1 cut(s)	965
BtgZI	GCGATG	1 cut(s)	531
BtsCI	GGATG	2 cut(s)	337, 500
BtsI	GCAGTG	1 cut(s)	564
BtsIMutI	CAGTG	3 cut(s)	111, 468, 564
Cac8I	GCNNGC	1 cut(s)	553
CaiI	CAGNNNCTG	1 cut(s)	730
CfoI	GCGC	2 cut(s)	557, 688
Cfr10I	RCCGGY	2 cut(s)	62, 427
Cfr13I	GGNCC	3 cut(s)	37, 169, 643
CseI	GACGC	4 cut(s)	88, 430, 801, 977
Csp6I	GTAC	3 cut(s)	620, 658, 830
CspAI	ACCGGT	1 cut(s)	427
CviAII	CATG	4 cut(s)	191, 246, 861, 966
CviQI	GTAC	3 cut(s)	620, 658, 830
DdeI	CTNAG	4 cut(s)	165, 375, 671, 957
DpnI	GATC	8 cut(s)	81, 158, 381, 489, 544, 599, 665, 783
DpnII	GATC	8 cut(s)	79, 156, 379, 487, 542, 597, 663, 781
EaeI	YGGCCR	1 cut(s)	88
EciI	GGCGGA	3 cut(s)	94, 344, 938
Eco130I	CCWWGG	1 cut(s)	965
Eco24I	GRGCYC	1 cut(s)	183
Eco47I	GGWCC	2 cut(s)	169, 643
Eco88I	CYCGRG	1 cut(s)	918
EcoNI	CCTNNNNNAGG	1 cut(s)	202
EcoO109I	RGGNCCY	2 cut(s)	169, 643
EcoRII	CCWGG	2 cut(s)	203, 491
EcoT14I	CCWWGG	1 cut(s)	965
EcoT38I	GRGCYC	1 cut(s)	183
ErhI	CCWWGG	1 cut(s)	965
Esp3I	CGTCTC	1 cut(s)	106
FaeI	CATG	4 cut(s)	194, 249, 864, 969
FaqI	GGGAC	2 cut(s)	83, 155
FatI	CATG	4 cut(s)	190, 245, 860, 965
FauI	CCCGC	1 cut(s)	59
Fnu4HI	GCNGC	4 cut(s)	46, 288, 531, 873
FokI	GGATG	2 cut(s)	344, 487
FriOI	GRGCYC	1 cut(s)	183
Fsp4HI	GCNGC	4 cut(s)	46, 288, 531, 873
FspBI	CTAG	2 cut(s)	236, 291
GlaI	GCGC	2 cut(s)	556, 687
GluI	GCNGC	4 cut(s)	46, 288, 531, 873
HaeIII	GGCC	3 cut(s)	39, 90, 360
HapII	CCGG	4 cut(s)	63, 428, 616, 689
HgaI	GACGC	4 cut(s)	88, 430, 801, 977
HhaI	GCGC	2 cut(s)	557, 688
Hin1I	GRCGYC	1 cut(s)	99
Hin1II	CATG	4 cut(s)	194, 249, 864, 969
Hin6I	GCGC	2 cut(s)	555, 686
HinP1I	GCGC	2 cut(s)	555, 686
HindIII	AAGCTT	2 cut(s)	16, 737
HinfI	GANTC	1 cut(s)	497
HpaII	CCGG	4 cut(s)	63, 428, 616, 689
HphI	GGTGA	1 cut(s)	151
Hpy188I	TCNGA	5 cut(s)	79, 376, 513, 547, 668
Hpy188III	TCNNGA	3 cut(s)	154, 418, 847
HpyAV	CCTTC	1 cut(s)	170
HpyCH4III	ACNGT	3 cut(s)	115, 463, 469
HpyCH4V	TGCA	7 cut(s)	45, 437, 449, 523, 584, 860, 899
HpyF10VI	GCNNNNNNNGC	5 cut(s)	45, 96, 187, 736, 801
HpyF3I	CTNAG	4 cut(s)	165, 375, 671, 957
Hsp92I	GRCGYC	1 cut(s)	99
Hsp92II	CATG	4 cut(s)	194, 249, 864, 969
HspAI	GCGC	2 cut(s)	555, 686
Kzo9I	GATC	8 cut(s)	79, 156, 379, 487, 542, 597, 663, 781
LmnI	GCTCC	2 cut(s)	727, 925
Lsp1109I	GCAGC	3 cut(s)	57, 517, 884
LweI	GCATC	4 cut(s)	424, 571, 595, 673
MaeI	CTAG	2 cut(s)	236, 291
MaeIII	GTNAC	2 cut(s)	241, 463
MalI	GATC	8 cut(s)	81, 158, 381, 489, 544, 599, 665, 783
MboI	GATC	8 cut(s)	79, 156, 379, 487, 542, 597, 663, 781
MboII	GAAGA	4 cut(s)	293, 607, 791, 920
MflI	RGATCY	3 cut(s)	379, 487, 663
MhlI	GDGCHC	1 cut(s)	183
MlsI	TGGCCA	1 cut(s)	90
MluCI	AATT	1 cut(s)	893
MluNI	TGGCCA	1 cut(s)	90
MlyI	GAGTC	1 cut(s)	506
MmeI	TCCRAC	2 cut(s)	407, 691
Mox20I	TGGCCA	1 cut(s)	90
MscI	TGGCCA	1 cut(s)	90
MseI	TTAA	1 cut(s)	735
Msp20I	TGGCCA	1 cut(s)	90
MspA1I	CMGCKG	1 cut(s)	530
MspI	CCGG	4 cut(s)	63, 428, 616, 689
MspR9I	CCNGG	3 cut(s)	205, 493, 617
MvaI	CCWGG	2 cut(s)	205, 493
MvnI	CGCG	1 cut(s)	555
MwoI	GCNNNNNNNGC	5 cut(s)	45, 96, 187, 736, 801
NciI	CCSGG	1 cut(s)	617
NcoI	CCATGG	1 cut(s)	965
NdeII	GATC	8 cut(s)	79, 156, 379, 487, 542, 597, 663, 781
NlaIII	CATG	4 cut(s)	194, 249, 864, 969
NlaIV	GGNNCC	5 cut(s)	29, 171, 381, 489, 665
NmuCI	GTSAC	2 cut(s)	241, 463
NspI	RCATGY	1 cut(s)	194
PasI	CCCWGGG	1 cut(s)	492
PcsI	WCGNNNNNNNCGW	2 cut(s)	876, 925
PfoI	TCCNGGA	1 cut(s)	203
PinAI	ACCGGT	1 cut(s)	427
PkrI	GCNGC	4 cut(s)	47, 289, 532, 874
PleI	GAGTC	1 cut(s)	505
PpsI	GAGTC	1 cut(s)	505
PpuMI	RGGWCCY	2 cut(s)	169, 643
Psp5II	RGGWCCY	2 cut(s)	169, 643
Psp6I	CCWGG	2 cut(s)	203, 491
PspGI	CCWGG	2 cut(s)	203, 491
PspN4I	GGNNCC	5 cut(s)	29, 171, 381, 489, 665
PspPI	GGNCC	3 cut(s)	37, 169, 643
PspPPI	RGGWCCY	2 cut(s)	169, 643
PstNI	CAGNNNCTG	1 cut(s)	730
PsuI	RGATCY	3 cut(s)	379, 487, 663
PvuII	CAGCTG	1 cut(s)	530
RsaI	GTAC	3 cut(s)	621, 659, 831
RsaNI	GTAC	3 cut(s)	620, 658, 830
SaqAI	TTAA	1 cut(s)	735
SatI	GCNGC	4 cut(s)	46, 288, 531, 873
Sau3AI	GATC	8 cut(s)	79, 156, 379, 487, 542, 597, 663, 781
Sau96I	GGNCC	3 cut(s)	37, 169, 643
SchI	GAGTC	1 cut(s)	506
ScrFI	CCNGG	3 cut(s)	205, 493, 617
SduI	GDGCHC	1 cut(s)	183
SfaNI	GCATC	4 cut(s)	424, 571, 595, 673
SgrAI	CRCCGGYG	1 cut(s)	62
SinI	GGWCC	2 cut(s)	169, 643
SmlI	CTYRAG	4 cut(s)	213, 396, 757, 845
SmoI	CTYRAG	4 cut(s)	213, 396, 757, 845
SpeI	ACTAGT	1 cut(s)	235
Sse9I	AATT	1 cut(s)	893
SsiI	CCGC	6 cut(s)	66, 105, 263, 287, 329, 923
SspI	AATATT	2 cut(s)	367, 628
SspMI	CTAG	2 cut(s)	236, 291
StyD4I	CCNGG	3 cut(s)	203, 491, 615
StyI	CCWWGG	1 cut(s)	965
TaaI	ACNGT	3 cut(s)	115, 463, 469
TaqI	TCGA	2 cut(s)	153, 322
TaqII	GACCGA	1 cut(s)	173
TasI	AATT	1 cut(s)	893
TatI	WGTACW	2 cut(s)	657, 829
TauI	GCSGC	1 cut(s)	290
Tru1I	TTAA	1 cut(s)	735
Tru9I	TTAA	1 cut(s)	735
TscAI	CASTG	3 cut(s)	118, 468, 564
TseFI	GTSAC	2 cut(s)	241, 463
TseI	GCWGC	3 cut(s)	45, 530, 872
Tsp45I	GTSAC	2 cut(s)	241, 463
TspDTI	ATGAA	1 cut(s)	936
TspRI	CASTG	3 cut(s)	118, 468, 564
VpaK11BI	GGWCC	2 cut(s)	169, 643
XagI	CCTNNNNNAGG	1 cut(s)	202
XapI	RAATTY	1 cut(s)	893
XceI	RCATGY	1 cut(s)	194
XcmI	CCANNNNNNNNNTGG	1 cut(s)	727
XspI	CTAG	2 cut(s)	236, 291

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.