FvH4_7g34250

QLQ

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0003697
Physical Location & Seq
Reverse (-)
24169853 .. 24173196
3344 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g34250.t1

Sequence Viewer

Length: 1005 bp
ATGAATAGTACTGGAGGTGGGGGGGGGGTTGGGGTTGGGGGTAACAGTACTGGGAATCTGATGGGAATGGGGATGGGGATGAGGTCACCTTTTACAGTGTCTCAGTGGCAAGAGCTGGAGCATCAGGCCCTGATCTTCAAGTATATGATGGCAGGCATGCCTGTGCCTCCTGATCTCGTGCTTCCTATTCAGAAGAGTTTTGACTCCATCTCTCAGAGATTCTTCCATCCCTCCATGGGTTATTGTTCCTTCTATGGGAAGAAGGTGGACCCGGAGCCAGGACGCTGCAGGAGGACTGATGGCAAGAAATGGAGGTGCTCAAAGGATGCATACCCCGACTCCAAGTACTGTGAGCGCCATATGCACCGTGGCCGCAACCGTTCAAGAAAGCCTGTGGAATCACAAACAATGACACAGTCATCGTCAACTGTGACATCCTTGATTGTCTCCGGAACCAGCAGTGGAACTGTGAGCTTCCAGACCCTTCCATTGAATTCCTTTGGGAATACGCAAGGCGCCAGTTCCGGAACCAATCAGTCTCATTATCAAATAGACTCCATTCCCTTTGGAATCCCAAACAAAGATTATAGGTATCTTCCAGGACTTAAACCTGATATGGGTGAGCATAGTTTTCTCTCTGATACTTCAGGAAGCAACAGGGGTCTCCACATGGACTCTCCATTGGACGGTACATGGCCTGTGATGCCATCCAGAGTTACCTCATTTCCTACATCAACATCGGGTGATGGCTCTGTATTGCAGAATGATTATCGACAGAATTCATTTTTCAATAATGATTTCCCCTCGGGGGAACCTTTGAAACATGAAGGTCAGCCTCTCCGACCTTTCTTTGATGAGTGGCCTAAAGGCCGAGATGCCTGGTCTGGTCTTGAGGATGAGAGATCCAACCAAACCTCATTCTCCACAACCCAGCTCTCAATATCAATTCCAATGACTTCTTCCGACTTCTCTGCAACAAGTTCTCAATCTCCACATGATAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
QLQ WRC

Protein Analysis

335

Amino Acids

36.74

Weight (kDa)

7.12

Isoelectric Point (pI)

46.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
QLQ PF08880 30 - 64 4.4e-16 QLQ
WRC PF08879 91 - 133 3.9e-21 WRC
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 515
AccIII TCCGGA 2 cut(s) 449, 524
AciI CCGC 1 cut(s) 373
AclWI GGATC 1 cut(s) 897
AcoI YGGCCR 1 cut(s) 370
AcsI RAATTY 2 cut(s) 493, 778
AcuI CTGAAG 1 cut(s) 630
AcyI GRCGYC 1 cut(s) 516
AfaI GTAC 4 cut(s) 10, 49, 347, 691
AfiI CCNNNNNNNGG 6 cut(s) 236, 255, 278, 617, 686, 808
AgsI TTSAA 5 cut(s) 139, 384, 493, 790, 820
AjnI CCWGG 3 cut(s) 277, 598, 878
AjuI GAANNNNNNNTTGG 2 cut(s) 943, 975
AluBI AGCT 3 cut(s) 115, 474, 934
AluI AGCT 3 cut(s) 115, 474, 934
Alw21I GWGCWC 1 cut(s) 320
Alw26I GTCTC 4 cut(s) 105, 451, 543, 668
AlwI GGATC 1 cut(s) 897
AlwNI CAGNNNCTG 1 cut(s) 130
Ama87I CYCGRG 1 cut(s) 805
Aor13HI TCCGGA 2 cut(s) 449, 524
AoxI GGCC 5 cut(s) 126, 370, 695, 860, 868
ApeKI GCWGC 1 cut(s) 285
ApoI RAATTY 2 cut(s) 493, 778
AspLEI GCGC 2 cut(s) 357, 518
AspS9I GGNCC 2 cut(s) 127, 268
AsuC2I CCSGG 1 cut(s) 272
AsuHPI GGTGA 3 cut(s) 78, 632, 755
AvaI CYCGRG 1 cut(s) 805
AvaII GGWCC 1 cut(s) 268
BanI GGYRCC 1 cut(s) 515
BauI CACGAG 1 cut(s) 176
Bbv12I GWGCWC 1 cut(s) 320
BbvI GCAGC 1 cut(s) 272
BccI CCATC 8 cut(s) 55, 67, 142, 215, 234, 293, 715, 740
BcgI CGANNNNNNTGC 2 cut(s) 953, 987
BciT130I CCWGG 3 cut(s) 279, 600, 880
BcnI CCSGG 1 cut(s) 272
BcoDI GTCTC 4 cut(s) 105, 451, 543, 668
BfmI CTRYAG 1 cut(s) 286
BfoI RGCGCY 2 cut(s) 358, 519
BisI GCNGC 2 cut(s) 286, 373
BlsI GCNGC 2 cut(s) 287, 374
BmcAI AGTACT 3 cut(s) 10, 49, 347
Bme1390I CCNGG 4 cut(s) 272, 279, 600, 880
Bme18I GGWCC 1 cut(s) 268
BmeT110I CYCGRG 1 cut(s) 805
BmgT120I GGNCC 2 cut(s) 127, 268
BmiI GGNNCC 6 cut(s) 270, 276, 454, 517, 529, 813
BmrFI CCNGG 4 cut(s) 272, 279, 600, 880
BmrI ACTGGG 1 cut(s) 60
BmsI GCATC 4 cut(s) 130, 316, 693, 865
BmuI ACTGGG 1 cut(s) 60
BpmI CTGGAG 2 cut(s) 33, 137
BpuEI CTTGAG 1 cut(s) 911
BpuMI CCSGG 1 cut(s) 272
BsaHI GRCGYC 1 cut(s) 516
BsaI GGTCTC 1 cut(s) 668
BsaJI CCNNGG 3 cut(s) 234, 367, 804
BsaWI WCCGGW 2 cut(s) 449, 524
BsaXI ACNNNNNCTCC 2 cut(s) 323, 353
Bsc4I CCNNNNNNNGG 6 cut(s) 236, 255, 278, 617, 686, 808
Bse1I ACTGG 3 cut(s) 16, 55, 519
BseAI TCCGGA 2 cut(s) 449, 524
BseBI CCWGG 3 cut(s) 279, 600, 880
BseDI CCNNGG 3 cut(s) 234, 367, 804
BseGI GGATG 7 cut(s) 78, 84, 226, 331, 434, 707, 901
BseLI CCNNNNNNNGG 6 cut(s) 236, 255, 278, 617, 686, 808
BseMII CTCAG 2 cut(s) 116, 227
BseNI ACTGG 3 cut(s) 16, 55, 519
BseXI GCAGC 1 cut(s) 272
BseYI CCCAGC 1 cut(s) 930
BshFI GGCC 5 cut(s) 128, 372, 697, 862, 870
BshNI GGYRCC 1 cut(s) 515
BsiHKAI GWGCWC 1 cut(s) 320
BsiHKCI CYCGRG 1 cut(s) 805
BsiSI CCGG 3 cut(s) 272, 450, 525
BslI CCNNNNNNNGG 6 cut(s) 236, 255, 278, 617, 686, 808
BsmAI GTCTC 4 cut(s) 105, 451, 543, 668
BsnI GGCC 5 cut(s) 128, 372, 697, 862, 870
Bso31I GGTCTC 1 cut(s) 668
BsoBI CYCGRG 1 cut(s) 805
Bsp1286I GDGCHC 1 cut(s) 320
Bsp13I TCCGGA 2 cut(s) 449, 524
Bsp143I GATC 3 cut(s) 132, 172, 902
Bsp19I CCATGG 1 cut(s) 234
BspACI CCGC 1 cut(s) 373
BspANI GGCC 5 cut(s) 128, 372, 697, 862, 870
BspCNI CTCAG 2 cut(s) 115, 226
BspEI TCCGGA 2 cut(s) 449, 524
BspLI GGNNCC 6 cut(s) 270, 276, 454, 517, 529, 813
BspMAI CTGCAG 1 cut(s) 290
BspPI GGATC 1 cut(s) 897
BspT107I GGYRCC 1 cut(s) 515
BspTNI GGTCTC 1 cut(s) 668
BsrI ACTGG 3 cut(s) 16, 55, 519
BssECI CCNNGG 3 cut(s) 234, 367, 804
BssMI GATC 3 cut(s) 132, 172, 902
BssNI GRCGYC 1 cut(s) 516
BssSI CACGAG 1 cut(s) 176
BssT1I CCWWGG 1 cut(s) 234
Bst2BI CACGAG 1 cut(s) 176
Bst2UI CCWGG 3 cut(s) 279, 600, 880
Bst4CI ACNGT 9 cut(s) 47, 97, 350, 368, 380, 417, 430, 469, 689
Bst6I CTCTTC 1 cut(s) 188
BstACI GRCGYC 1 cut(s) 516
BstC8I GCNNGC 2 cut(s) 154, 158
BstDEI CTNAG 2 cut(s) 102, 213
BstDSI CCRYGG 2 cut(s) 234, 367
BstEII GGTNACC 1 cut(s) 84
BstF5I GGATG 7 cut(s) 78, 84, 226, 331, 434, 707, 901
BstH2I RGCGCY 2 cut(s) 358, 519
BstHHI GCGC 2 cut(s) 357, 518
BstKTI GATC 3 cut(s) 135, 175, 905
BstMAI GTCTC 4 cut(s) 105, 451, 543, 668
BstMBI GATC 3 cut(s) 132, 172, 902
BstMWI GCNNNNNNNGC 2 cut(s) 361, 703
BstNI CCWGG 3 cut(s) 279, 600, 880
BstNSI RCATGY 1 cut(s) 160
BstPI GGTNACC 1 cut(s) 84
BstSCI CCNGG 4 cut(s) 270, 277, 598, 878
BstSFI CTRYAG 1 cut(s) 286
BstV1I GCAGC 1 cut(s) 272
BstX2I RGATCY 1 cut(s) 902
BstYI RGATCY 1 cut(s) 902
BsuRI GGCC 5 cut(s) 128, 372, 697, 862, 870
BtgI CCRYGG 2 cut(s) 234, 367
BtsCI GGATG 7 cut(s) 78, 84, 226, 331, 434, 707, 901
BtsI GCAGTG 1 cut(s) 466
BtsIMutI CAGTG 3 cut(s) 102, 110, 466
Cac8I GCNNGC 2 cut(s) 154, 158
CaiI CAGNNNCTG 1 cut(s) 130
CfoI GCGC 2 cut(s) 357, 518
Cfr13I GGNCC 2 cut(s) 127, 268
CseI GACGC 1 cut(s) 291
Csp6I GTAC 4 cut(s) 9, 48, 346, 690
CviAII CATG 6 cut(s) 157, 235, 670, 693, 824, 995
CviQI GTAC 4 cut(s) 9, 48, 346, 690
DdeI CTNAG 2 cut(s) 102, 213
DinI GGCGCC 1 cut(s) 517
DpnI GATC 3 cut(s) 134, 174, 904
DpnII GATC 3 cut(s) 132, 172, 902
EaeI YGGCCR 1 cut(s) 370
Eam1104I CTCTTC 1 cut(s) 188
EarI CTCTTC 1 cut(s) 188
Eco130I CCWWGG 1 cut(s) 234
Eco31I GGTCTC 1 cut(s) 668
Eco47I GGWCC 1 cut(s) 268
Eco57I CTGAAG 1 cut(s) 630
Eco88I CYCGRG 1 cut(s) 805
Eco91I GGTNACC 1 cut(s) 84
EcoO109I RGGNCCY 1 cut(s) 127
EcoO65I GGTNACC 1 cut(s) 84
EcoRI GAATTC 2 cut(s) 493, 778
EcoRII CCWGG 3 cut(s) 277, 598, 878
EcoT14I CCWWGG 1 cut(s) 234
EcoT22I ATGCAT 1 cut(s) 331
EgeI GGCGCC 1 cut(s) 517
EheI GGCGCC 1 cut(s) 517
ErhI CCWWGG 1 cut(s) 234
FaeI CATG 6 cut(s) 160, 238, 673, 696, 827, 998
FatI CATG 6 cut(s) 156, 234, 669, 692, 823, 994
FauNDI CATATG 1 cut(s) 360
Fnu4HI GCNGC 2 cut(s) 286, 373
FokI GGATG 7 cut(s) 85, 91, 213, 338, 421, 694, 908
Fsp4HI GCNGC 2 cut(s) 286, 373
GlaI GCGC 2 cut(s) 356, 517
GluI GCNGC 2 cut(s) 286, 373
GsaI CCCAGC 1 cut(s) 934
GsuI CTGGAG 2 cut(s) 33, 137
HaeII RGCGCY 2 cut(s) 358, 519
HaeIII GGCC 5 cut(s) 128, 372, 697, 862, 870
HapII CCGG 3 cut(s) 272, 450, 525
HgaI GACGC 1 cut(s) 291
HhaI GCGC 2 cut(s) 357, 518
Hin1I GRCGYC 1 cut(s) 516
Hin1II CATG 6 cut(s) 160, 238, 673, 696, 827, 998
Hin6I GCGC 2 cut(s) 355, 516
HinP1I GCGC 2 cut(s) 355, 516
HincII GTYRAC 1 cut(s) 426
HindII GTYRAC 1 cut(s) 426
HinfI GANTC 8 cut(s) 55, 203, 219, 338, 398, 554, 570, 674
HpaII CCGG 3 cut(s) 272, 450, 525
HphI GGTGA 3 cut(s) 78, 632, 755
Hpy166II GTNNAC 2 cut(s) 268, 426
Hpy188I TCNGA 6 cut(s) 60, 192, 216, 640, 842, 964
Hpy188III TCNNGA 8 cut(s) 170, 384, 450, 478, 525, 648, 711, 890
Hpy8I GTNNAC 2 cut(s) 268, 426
HpyAV CCTTC 4 cut(s) 256, 259, 494, 821
HpyCH4III ACNGT 9 cut(s) 47, 97, 350, 368, 380, 417, 430, 469, 689
HpyCH4V TGCA 5 cut(s) 288, 329, 364, 760, 974
HpyF10VI GCNNNNNNNGC 2 cut(s) 361, 703
HpyF3I CTNAG 2 cut(s) 102, 213
Hsp92I GRCGYC 1 cut(s) 516
Hsp92II CATG 6 cut(s) 160, 238, 673, 696, 827, 998
HspAI GCGC 2 cut(s) 355, 516
KasI GGCGCC 1 cut(s) 515
Kpn2I TCCGGA 2 cut(s) 449, 524
Kzo9I GATC 3 cut(s) 132, 172, 902
LmnI GCTCC 2 cut(s) 118, 274
Lsp1109I GCAGC 1 cut(s) 272
LweI GCATC 4 cut(s) 130, 316, 693, 865
MaeIII GTNAC 4 cut(s) 41, 84, 430, 715
MalI GATC 3 cut(s) 134, 174, 904
MboI GATC 3 cut(s) 132, 172, 902
MboII GAAGA 6 cut(s) 127, 205, 214, 271, 587, 951
MflI RGATCY 1 cut(s) 902
MhlI GDGCHC 1 cut(s) 320
MluCI AATT 4 cut(s) 493, 778, 945, 1000
Mly113I GGCGCC 1 cut(s) 516
MlyI GAGTC 4 cut(s) 197, 332, 548, 668
MmeI TCCRAC 3 cut(s) 865, 930, 987
Mph1103I ATGCAT 1 cut(s) 331
MroI TCCGGA 2 cut(s) 449, 524
MseI TTAA 1 cut(s) 606
MslI CAYNNNNRTG 1 cut(s) 161
MspI CCGG 3 cut(s) 272, 450, 525
MspR9I CCNGG 4 cut(s) 272, 279, 600, 880
MvaI CCWGG 3 cut(s) 279, 600, 880
MwoI GCNNNNNNNGC 2 cut(s) 361, 703
NarI GGCGCC 1 cut(s) 516
NciI CCSGG 1 cut(s) 272
NcoI CCATGG 1 cut(s) 234
NdeI CATATG 1 cut(s) 360
NdeII GATC 3 cut(s) 132, 172, 902
NlaIII CATG 6 cut(s) 160, 238, 673, 696, 827, 998
NlaIV GGNNCC 6 cut(s) 270, 276, 454, 517, 529, 813
NmeAIII GCCGAG 1 cut(s) 896
NmuCI GTSAC 2 cut(s) 84, 430
NsiI ATGCAT 1 cut(s) 331
NspI RCATGY 1 cut(s) 160
PaeI GCATGC 1 cut(s) 160
PfeI GAWTC 4 cut(s) 55, 219, 398, 570
PflFI GACNNNGTC 1 cut(s) 415
PfoI TCCNGGA 1 cut(s) 598
PkrI GCNGC 2 cut(s) 287, 374
PleI GAGTC 4 cut(s) 197, 332, 548, 668
PluTI GGCGCC 1 cut(s) 519
PpsI GAGTC 4 cut(s) 197, 332, 548, 668
Psp6I CCWGG 3 cut(s) 277, 598, 878
PspEI GGTNACC 1 cut(s) 84
PspFI CCCAGC 1 cut(s) 930
PspGI CCWGG 3 cut(s) 277, 598, 878
PspN4I GGNNCC 6 cut(s) 270, 276, 454, 517, 529, 813
PspPI GGNCC 2 cut(s) 127, 268
PstI CTGCAG 1 cut(s) 290
PstNI CAGNNNCTG 1 cut(s) 130
PsuI RGATCY 1 cut(s) 902
PsyI GACNNNGTC 1 cut(s) 415
RsaI GTAC 4 cut(s) 10, 49, 347, 691
RsaNI GTAC 4 cut(s) 9, 48, 346, 690
RseI CAYNNNNRTG 1 cut(s) 161
SaqAI TTAA 1 cut(s) 606
SatI GCNGC 2 cut(s) 286, 373
Sau3AI GATC 3 cut(s) 132, 172, 902
Sau96I GGNCC 2 cut(s) 127, 268
ScaI AGTACT 3 cut(s) 10, 49, 347
SchI GAGTC 4 cut(s) 197, 332, 548, 668
ScrFI CCNGG 4 cut(s) 272, 279, 600, 880
SduI GDGCHC 1 cut(s) 320
SfaNI GCATC 4 cut(s) 130, 316, 693, 865
SfcI CTRYAG 1 cut(s) 286
SfoI GGCGCC 1 cut(s) 517
SinI GGWCC 1 cut(s) 268
SmiMI CAYNNNNRTG 1 cut(s) 161
SmlI CTYRAG 1 cut(s) 890
SmoI CTYRAG 1 cut(s) 890
SphI GCATGC 1 cut(s) 160
Sse9I AATT 4 cut(s) 493, 778, 945, 1000
SsiI CCGC 1 cut(s) 373
SspDI GGCGCC 1 cut(s) 515
StyD4I CCNGG 4 cut(s) 270, 277, 598, 878
StyI CCWWGG 1 cut(s) 234
TaaI ACNGT 9 cut(s) 47, 97, 350, 368, 380, 417, 430, 469, 689
TaqI TCGA 1 cut(s) 772
TasI AATT 4 cut(s) 493, 778, 945, 1000
TatI WGTACW 3 cut(s) 8, 47, 345
TauI GCSGC 1 cut(s) 375
TfiI GAWTC 4 cut(s) 55, 219, 398, 570
Tru1I TTAA 1 cut(s) 606
Tru9I TTAA 1 cut(s) 606
TscAI CASTG 3 cut(s) 102, 110, 466
TseFI GTSAC 2 cut(s) 84, 430
TseI GCWGC 1 cut(s) 285
Tsp45I GTSAC 2 cut(s) 84, 430
TspDTI ATGAA 3 cut(s) 17, 771, 840
TspRI CASTG 3 cut(s) 102, 110, 466
Tth111I GACNNNGTC 1 cut(s) 415
VpaK11BI GGWCC 1 cut(s) 268
XapI RAATTY 2 cut(s) 493, 778
XceI RCATGY 1 cut(s) 160
XcmI CCANNNNNNNNNTGG 1 cut(s) 365
ZrmI AGTACT 3 cut(s) 10, 49, 347
Zsp2I ATGCAT 1 cut(s) 331
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.